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CYP27B1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol CYP27B1
Entrez Gene ID 1594
Full Name cytochrome P450, family 27, subfamily B, polypeptide 1
Synonyms CP2B, CYP1, CYP1alpha, CYP27B, P450c1, PDDR, VDD1, VDDR, VDDRI, VDR
General protein information
Preferred Names
25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
Names
25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
1alpha(OH)ase
VD3 1A hydroxylase
cytochrome p450 27B1
cytochrome P450C1 alpha
cytochrome P450VD1-alpha
calcidiol 1-monooxygenase
25-OHD-1 alpha-hydroxylase
25 hydroxyvitamin D3-1-alpha hydroxylase
cytochrome P450 subfamily XXVIIB polypeptide 1
NP_000776.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

12

12q14.1

Summary This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

609506

Disorder Html: Vitamin D-dependent rickets, type I, 264700 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_000785 NP_000776 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial



Homo sapiens (human) CYP27B1 NP_000776.1
Pan troglodytes (chimpanzee) CYP27B1 XP_509175.2
Macaca mulatta (Rhesus monkey) CYP27B1 XP_001116450.1
Canis lupus familiaris (dog) CYP27B1 XP_538254.3
Bos taurus (cattle) CYP27B1 NP_001179213.1
Mus musculus (house mouse) Cyp27b1 NP_034139.2
Rattus norvegicus (Norway rat) Cyp27b1 NP_446215.1
Danio rerio (zebrafish) LOC793309 XP_003199448.2
Xenopus (Silurana) tropicalis (western clawed frog) cyp27b1 NP_001006907.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CYP27B1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CYP27B1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu27370
NM_000785 Homo sapiens cytochrome P450, family 27, subfamily B, polypeptide 1 (CYP27B1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$219.50-$307.30
$439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu27370
Clone ID Related Accession (Same CDS sequence) NM_000785
Accession Version NM_000785.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1527bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP296031.1, AB005038.1 and AW449645.1. This sequence is a reference standard in the RefSeqGene project. On Sep 2, 2005 this sequence version replaced gi:13904864. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AB005038.1, AF020192.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGACCCAGA CCCTCAAGTA CGCCTCCAGA GTGTTCCATC GCGTCCGCTG GGCGCCCGAG 
TTGGGCGCCT CCCTAGGCTA CCGAGAGTAC CACTCAGCAC GCCGGAGCTT GGCAGACATC
CCAGGCCCCT CTACGCCCAG CTTTCTGGCC GAACTTTTCT GCAAGGGGGG GCTGTCGAGG
CTACACGAGC TGCAGGTGCA GGGCGCCGCG CACTTCGGGC CGGTGTGGCT AGCCAGCTTT
GGGACAGTGC GCACCGTGTA CGTGGCTGCC CCTGCACTCG TCGAGGAGCT GCTGCGACAG
GAGGGACCCC GGCCCGAGCG CTGCAGCTTC TCGCCCTGGA CGGAGCACCG CCGCTGCCGC
CAGCGGGCTT GCGGACTGCT CACTGCGGAA GGCGAAGAAT GGCAAAGGCT CCGCAGTCTC
CTGGCCCCGC TCCTCCTCCG GCCTCAAGCG GCCGCCCGCT ACGCCGGAAC CCTGAACAAC
GTAGTCTGCG ACCTTGTGCG GCGTCTGAGG CGCCAGCGGG GACGTGGCAC GGGGCCGCCC
GCCCTGGTTC GGGACGTGGC GGGGGAATTT TACAAGTTCG GACTGGAAGG CATCGCCGCG
GTTCTGCTCG GCTCGCGCTT GGGCTGCCTG GAGGCTCAAG TGCCACCCGA CACGGAGACC
TTCATCCGCG CTGTGGGCTC GGTGTTTGTG TCCACGCTGT TGACCATGGC GATGCCCCAC
TGGCTGCGCC ACCTTGTGCC TGGGCCCTGG GGCCGCCTCT GCCGAGACTG GGACCAGATG
TTTGCATTTG CTCAGAGGCA CGTGGAGCGG CGAGAGGCAG AGGCAGCCAT GAGGAACGGA
GGACAGCCCG AGAAGGACCT GGAGTCTGGG GCGCACCTGA CCCACTTCCT GTTCCGGGAA
GAGTTGCCTG CCCAGTCCAT CCTGGGAAAT GTGACAGAGT TGCTATTGGC GGGAGTGGAC
ACGGTGTCCA ACACGCTCTC TTGGGCTCTG TATGAGCTCT CCCGGCACCC CGAAGTCCAG
ACAGCACTCC ACTCAGAGAT CACAGCTGCC CTGAGCCCTG GCTCCAGTGC CTACCCCTCA
GCCACTGTTC TGTCCCAGCT GCCCCTGCTG AAGGCGGTGG TCAAGGAAGT GCTAAGACTG
TACCCTGTGG TACCTGGAAA TTCTCGTGTC CCAGACAAAG ACATTCATGT GGGTGACTAT
ATTATCCCCA AAAATACGCT GGTCACTCTG TGTCACTATG CCACTTCAAG GGACCCTGCC
CAGTTCCCAG AGCCAAATTC TTTTCGTCCA GCTCGCTGGC TGGGGGAGGG TCCCACCCCC
CACCCATTTG CATCTCTTCC CTTTGGCTTT GGCAAGCGCA GCTGTATGGG GAGACGCCTG
GCAGAGCTTG AATTGCAAAT GGCTTTGGCC CAGATCCTAA CACATTTTGA GGTGCAGCCT
GAGCCAGGTG CGGCCCCAGT TAGACCCAAG ACCCGGACTG TCCTGGTACC TGAAAGGAGC
ATCAACCTAC AGTTTTTGGA CAGATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000776.1
CDS153..1679
Misc Feature(1)105..107(+)
Misc Feature(2)273..1667(+)
Misc Feature(3)1515..1517(+)
Exon (1)1..347
Gene:CYP27B1
Gene Synonym:
Exon (2)348..538
Gene:CYP27B1
Gene Synonym:
Exon (3)539..741
Gene:CYP27B1
Gene Synonym:
Exon (4)742..942
Gene:CYP27B1
Gene Synonym:
Exon (5)943..1115
Gene:CYP27B1
Gene Synonym:
Exon (6)1116..1288
Gene:CYP27B1
Gene Synonym:
Exon (7)1289..1367
Gene:CYP27B1
Gene Synonym:
Exon (8)1368..1565
Gene:CYP27B1
Gene Synonym:
Exon (9)1566..2487
Gene:CYP27B1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000785
Organism Homo sapiens (human)
Definition Homo sapiens cytochrome P450, family 27, subfamily B, polypeptide 1 (CYP27B1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000785

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGACCCAGA CCCTCAAGTA CGCCTCCAGA GTGTTCCATC GCGTCCGCTG GGCGCCCGAG 
TTGGGCGCCT CCCTAGGCTA CCGAGAGTAC CACTCAGCAC GCCGGAGCTT GGCAGACATC
CCAGGCCCCT CTACGCCCAG CTTTCTGGCC GAACTTTTCT GCAAGGGGGG GCTGTCGAGG
CTACACGAGC TGCAGGTGCA GGGCGCCGCG CACTTCGGGC CGGTGTGGCT AGCCAGCTTT
GGGACAGTGC GCACCGTGTA CGTGGCTGCC CCTGCACTCG TCGAGGAGCT GCTGCGACAG
GAGGGACCCC GGCCCGAGCG CTGCAGCTTC TCGCCCTGGA CGGAGCACCG CCGCTGCCGC
CAGCGGGCTT GCGGACTGCT CACTGCGGAA GGCGAAGAAT GGCAAAGGCT CCGCAGTCTC
CTGGCCCCGC TCCTCCTCCG GCCTCAAGCG GCCGCCCGCT ACGCCGGAAC CCTGAACAAC
GTAGTCTGCG ACCTTGTGCG GCGTCTGAGG CGCCAGCGGG GACGTGGCAC GGGGCCGCCC
GCCCTGGTTC GGGACGTGGC GGGGGAATTT TACAAGTTCG GACTGGAAGG CATCGCCGCG
GTTCTGCTCG GCTCGCGCTT GGGCTGCCTG GAGGCTCAAG TGCCACCCGA CACGGAGACC
TTCATCCGCG CTGTGGGCTC GGTGTTTGTG TCCACGCTGT TGACCATGGC GATGCCCCAC
TGGCTGCGCC ACCTTGTGCC TGGGCCCTGG GGCCGCCTCT GCCGAGACTG GGACCAGATG
TTTGCATTTG CTCAGAGGCA CGTGGAGCGG CGAGAGGCAG AGGCAGCCAT GAGGAACGGA
GGACAGCCCG AGAAGGACCT GGAGTCTGGG GCGCACCTGA CCCACTTCCT GTTCCGGGAA
GAGTTGCCTG CCCAGTCCAT CCTGGGAAAT GTGACAGAGT TGCTATTGGC GGGAGTGGAC
ACGGTGTCCA ACACGCTCTC TTGGGCTCTG TATGAGCTCT CCCGGCACCC CGAAGTCCAG
ACAGCACTCC ACTCAGAGAT CACAGCTGCC CTGAGCCCTG GCTCCAGTGC CTACCCCTCA
GCCACTGTTC TGTCCCAGCT GCCCCTGCTG AAGGCGGTGG TCAAGGAAGT GCTAAGACTG
TACCCTGTGG TACCTGGAAA TTCTCGTGTC CCAGACAAAG ACATTCATGT GGGTGACTAT
ATTATCCCCA AAAATACGCT GGTCACTCTG TGTCACTATG CCACTTCAAG GGACCCTGCC
CAGTTCCCAG AGCCAAATTC TTTTCGTCCA GCTCGCTGGC TGGGGGAGGG TCCCACCCCC
CACCCATTTG CATCTCTTCC CTTTGGCTTT GGCAAGCGCA GCTGTATGGG GAGACGCCTG
GCAGAGCTTG AATTGCAAAT GGCTTTGGCC CAGATCCTAA CACATTTTGA GGTGCAGCCT
GAGCCAGGTG CGGCCCCAGT TAGACCCAAG ACCCGGACTG TCCTGGTACC TGAAAGGAGC
ATCAACCTAC AGTTTTTGGA CAGATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Characterization of vitamin D production by human ocular barrier cells
Invest. Ophthalmol. Vis. Sci. 55 (4), 2140-2147 (2014)
Alsalem JA, Patel D, Susarla R, Coca-Prados M, Bland R, Walker EA, Rauz S and Wallace GR.


book

Analysis of CYP27B1 in multiple sclerosis
J. Neuroimmunol. 266 (1-2), 64-66 (2014)
Ross JP, Bernales CQ, Lee JD, Sadovnick AD, Traboulsee AL and Vilarino-Guell C.


book

[Vitamin D in the etiopathogenesis of autoimmune thyroiditis]
Pol. Merkur. Lekarski 34 (203), 281-285 (2013)
Lacka K and Maciejewski A.


book

Association between polymorphism of the vitamin D metabolism gene CYP27B1 and HLA-B27-associated uveitis. Is a state of relative immunodeficiency pathogenic in HLA B27-positive uveitis?
PLoS ONE 8 (4), E62244 (2013)
Steinwender G, Lindner E, Weger M, Plainer S, Renner W, Ardjomand N and El-Shabrawi Y.


book

No evidence for a role of rare CYP27B1 functional variations in multiple sclerosis
Ann. Neurol. 73 (3), 433-437 (2013)
Barizzone N, Pauwels I, Luciano B, Franckaert D, Guerini FR, Cosemans L, Hilven K, Salviati A, Dooley J, Danso-Abeam D, di Sapio A, Cavalla P, Decallonne B, Mathieu C, Liston A, Leone M, Dubois B, D'Alfonso S and Goris A.


book

25-Hydroxyvitamin D3 1alpha-hydroxylase and vitamin D synthesis
Science 277 (5333), 1827-1830 (1997)
Takeyama K, Kitanaka S, Sato T, Kobori M, Yanagisawa J and Kato S.


book

HIV-1 p17 and IFN-gamma both induce fructose 1,6-bisphosphatase
J. Interferon Cytokine Res. 17 (8), 461-467 (1997)
Besancon F, Just J, Bourgeade MF, Van Weyenbergh J, Solomon D, Guillozo H, Wietzerbin J and Cayre YE.


book

The Sp1 transcription factor gene (SP1) and the 1,25-dihydroxyvitamin D3 receptor gene (VDR) are colocalized on human chromosome arm 12q and rat chromosome 7
Genomics 11 (1), 168-173 (1991)
Szpirer J, Szpirer C, Riviere M, Levan G, Marynen P, Cassiman JJ, Wiese R and DeLuca HF.


book

Mapping autosomal recessive vitamin D dependency type I to chromosome 12q14 by linkage analysis
Am. J. Hum. Genet. 47 (1), 28-36 (1990)
Labuda M, Morgan K and Glorieux FH.


book

Tumor necrosis factor-alpha induces vitamin D-1-hydroxylase activity in normal human alveolar macrophages
J. Cell. Physiol. 142 (3), 652-656 (1990)
Pryke AM, Duggan C, White CP, Posen S and Mason RS.


 
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