×

AGT cDNA ORF clone, Homo sapiens (human)

Gene Symbol AGT
Entrez Gene ID 183
Full Name angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
Synonyms ANHU, SERPINA8
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1q42.2

Summary The protein encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

106150

Disorder Html: {Hypertension, essential, susceptibility to}, 145500 (3);

mRNA and Protein(s)

mRNA Protein Name
NM_000029 NP_000020 angiotensinogen preproprotein



Homo sapiens (human) AGT NP_000020.1
Pan troglodytes (chimpanzee) AGT NP_001009032.1
Macaca mulatta (Rhesus monkey) AGT XP_001107374.2
Canis lupus familiaris (dog) AGT XP_005618890.1
Mus musculus (house mouse) Agt NP_031454.3
Rattus norvegicus (Norway rat) Agt NP_602308.1
Gallus gallus (chicken) AGT XP_004935548.1
Danio rerio (zebrafish) agt NP_932329.1
Xenopus (Silurana) tropicalis (western clawed frog) agt XP_002931552.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AGT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AGT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Free ORF Clone
for Every Scientist
More Detail >>

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu26781
NM_000029 Homo sapiens angiotensinogen (serpin peptidase inhibitor, clade A, member 8) (AGT), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu26781
Clone ID Related Accession (Same CDS sequence) NM_000029
Accession Version NM_000029.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1458bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product angiotensinogen preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC412932.1, AK312291.1, K02215.1, T97863.1 and AW128887.1. This sequence is a reference standard in the RefSeqGene project. On May 21, 2008 this sequence version replaced gi:73622269. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: K02215.1, AK222815.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGCGGAAGC GAGCACCCCA GTCTGAGATG GCTCCTGCCG GTGTGAGCCT GAGGGCCACC 
ATCCTCTGCC TCCTGGCCTG GGCTGGCCTG GCTGCAGGTG ACCGGGTGTA CATACACCCC
TTCCACCTCG TCATCCACAA TGAGAGTACC TGTGAGCAGC TGGCAAAGGC CAATGCCGGG
AAGCCCAAAG ACCCCACCTT CATACCTGCT CCAATTCAGG CCAAGACATC CCCTGTGGAT
GAAAAGGCCC TACAGGACCA GCTGGTGCTA GTCGCTGCAA AACTTGACAC CGAAGACAAG
TTGAGGGCCG CAATGGTCGG GATGCTGGCC AACTTCTTGG GCTTCCGTAT ATATGGCATG
CACAGTGAGC TATGGGGCGT GGTCCATGGG GCCACCGTCC TCTCCCCAAC GGCTGTCTTT
GGCACCCTGG CCTCTCTCTA TCTGGGAGCC TTGGACCACA CAGCTGACAG GCTACAGGCA
ATCCTGGGTG TTCCTTGGAA GGACAAGAAC TGCACCTCCC GGCTGGATGC GCACAAGGTC
CTGTCTGCCC TGCAGGCTGT ACAGGGCCTG CTAGTGGCCC AGGGCAGGGC TGATAGCCAG
GCCCAGCTGC TGCTGTCCAC GGTGGTGGGC GTGTTCACAG CCCCAGGCCT GCACCTGAAG
CAGCCGTTTG TGCAGGGCCT GGCTCTCTAT ACCCCTGTGG TCCTCCCACG CTCTCTGGAC
TTCACAGAAC TGGATGTTGC TGCTGAGAAG ATTGACAGGT TCATGCAGGC TGTGACAGGA
TGGAAGACTG GCTGCTCCCT GATGGGAGCC AGTGTGGACA GCACCCTGGC TTTCAACACC
TACGTCCACT TCCAAGGGAA GATGAAGGGC TTCTCCCTGC TGGCCGAGCC CCAGGAGTTC
TGGGTGGACA ACAGCACCTC AGTGTCTGTT CCCATGCTCT CTGGCATGGG CACCTTCCAG
CACTGGAGTG ACATCCAGGA CAACTTCTCG GTGACTCAAG TGCCCTTCAC TGAGAGCGCC
TGCCTGCTGC TGATCCAGCC TCACTATGCC TCTGACCTGG ACAAGGTGGA GGGTCTCACT
TTCCAGCAAA ACTCCCTCAA CTGGATGAAG AAACTATCTC CCCGGACCAT CCACCTGACC
ATGCCCCAAC TGGTGCTGCA AGGATCTTAT GACCTGCAGG ACCTGCTCGC CCAGGCTGAG
CTGCCCGCCA TTCTGCACAC CGAGCTGAAC CTGCAAAAAT TGAGCAATGA CCGCATCAGG
GTGGGGGAGG TGCTGAACAG CATTTTTTTT GAGCTTGAAG CGGATGAGAG AGAGCCCACA
GAGTCTACCC AACAGCTTAA CAAGCCTGAG GTCTTGGAGG TGACCCTGAA CCGCCCATTC
CTGTTTGCTG TGTATGATCA AAGCGCCACT GCCCTGCACT TCCTGGGCCG CGTGGCCAAC
CCGCTGAGCA CAGCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000020.1
CDS509..1966
Misc Feature(1)455..457(+)
Misc Feature(2)626..628(+)
Misc Feature(3)626..628(+)
Misc Feature(4)629..631(+)
Misc Feature(5)629..631(+)
Misc Feature(6)632..634(+)
Misc Feature(7)647..649(+)
Misc Feature(8)821..1951(+)
Misc Feature(9)1016..1018(+)
Misc Feature(10)1418..1420(+)
Misc Feature(11)1490..1492(+)
Exon (1)1..505
Gene:AGT
Gene Synonym:
Exon (2)506..1364
Gene:AGT
Gene Synonym:
Exon (3)1365..1632
Gene:AGT
Gene Synonym:
Exon (4)1633..1777
Gene:AGT
Gene Synonym:
Exon (5)1778..2584
Gene:AGT
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000029
Organism Homo sapiens (human)
Definition Homo sapiens angiotensinogen (serpin peptidase inhibitor, clade A, member 8) (AGT), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000029

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGCGGAAGC GAGCACCCCA GTCTGAGATG GCTCCTGCCG GTGTGAGCCT GAGGGCCACC 
ATCCTCTGCC TCCTGGCCTG GGCTGGCCTG GCTGCAGGTG ACCGGGTGTA CATACACCCC
TTCCACCTCG TCATCCACAA TGAGAGTACC TGTGAGCAGC TGGCAAAGGC CAATGCCGGG
AAGCCCAAAG ACCCCACCTT CATACCTGCT CCAATTCAGG CCAAGACATC CCCTGTGGAT
GAAAAGGCCC TACAGGACCA GCTGGTGCTA GTCGCTGCAA AACTTGACAC CGAAGACAAG
TTGAGGGCCG CAATGGTCGG GATGCTGGCC AACTTCTTGG GCTTCCGTAT ATATGGCATG
CACAGTGAGC TATGGGGCGT GGTCCATGGG GCCACCGTCC TCTCCCCAAC GGCTGTCTTT
GGCACCCTGG CCTCTCTCTA TCTGGGAGCC TTGGACCACA CAGCTGACAG GCTACAGGCA
ATCCTGGGTG TTCCTTGGAA GGACAAGAAC TGCACCTCCC GGCTGGATGC GCACAAGGTC
CTGTCTGCCC TGCAGGCTGT ACAGGGCCTG CTAGTGGCCC AGGGCAGGGC TGATAGCCAG
GCCCAGCTGC TGCTGTCCAC GGTGGTGGGC GTGTTCACAG CCCCAGGCCT GCACCTGAAG
CAGCCGTTTG TGCAGGGCCT GGCTCTCTAT ACCCCTGTGG TCCTCCCACG CTCTCTGGAC
TTCACAGAAC TGGATGTTGC TGCTGAGAAG ATTGACAGGT TCATGCAGGC TGTGACAGGA
TGGAAGACTG GCTGCTCCCT GATGGGAGCC AGTGTGGACA GCACCCTGGC TTTCAACACC
TACGTCCACT TCCAAGGGAA GATGAAGGGC TTCTCCCTGC TGGCCGAGCC CCAGGAGTTC
TGGGTGGACA ACAGCACCTC AGTGTCTGTT CCCATGCTCT CTGGCATGGG CACCTTCCAG
CACTGGAGTG ACATCCAGGA CAACTTCTCG GTGACTCAAG TGCCCTTCAC TGAGAGCGCC
TGCCTGCTGC TGATCCAGCC TCACTATGCC TCTGACCTGG ACAAGGTGGA GGGTCTCACT
TTCCAGCAAA ACTCCCTCAA CTGGATGAAG AAACTATCTC CCCGGACCAT CCACCTGACC
ATGCCCCAAC TGGTGCTGCA AGGATCTTAT GACCTGCAGG ACCTGCTCGC CCAGGCTGAG
CTGCCCGCCA TTCTGCACAC CGAGCTGAAC CTGCAAAAAT TGAGCAATGA CCGCATCAGG
GTGGGGGAGG TGCTGAACAG CATTTTTTTT GAGCTTGAAG CGGATGAGAG AGAGCCCACA
GAGTCTACCC AACAGCTTAA CAAGCCTGAG GTCTTGGAGG TGACCCTGAA CCGCCCATTC
CTGTTTGCTG TGTATGATCA AAGCGCCACT GCCCTGCACT TCCTGGGCCG CGTGGCCAAC
CCGCTGAGCA CAGCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Revising angiotensinogen from phylogenetic and genetic variants perspectives
Biochem. Biophys. Res. Commun. 446 (2), 504-518 (2014)
Kumar A, Sarde SJ and Bhandari A.


book

Dynamic CCAAT/enhancer binding protein-associated changes of DNA methylation in the angiotensinogen gene
Hypertension 63 (2), 281-288 (2014)
Wang F, Demura M, Cheng Y, Zhu A, Karashima S, Yoneda T, Demura Y, Maeda Y, Namiki M, Ono K, Nakamura Y, Sasano H, Akagi T, Yamagishi M, Saijoh K and Takeda Y.


book

An introduction of genetics in otosclerosis: a systematic review
Otolaryngol Head Neck Surg 150 (1), 34-39 (2014)
Bittermann AJ, Wegner I, Noordman BJ, Vincent R, van der Heijden GJ and Grolman W.


book

Correlation analysis of angiotensin-converting enzyme, angiotensinogen, and endothelial nitric oxide synthase gene polymorphisms and the progression of immunoglobulin A nephropathy/membranous nephropathy
Hum. Pathol. 44 (12), 2806-2813 (2013)
Zhu X, Kong D, Zhang L, Sun Y, Na S, Han C and Jin X.


book

Update of the human and mouse SERPIN gene superfamily
Hum. Genomics 7, 22 (2013)
Heit C, Jackson BC, McAndrews M, Wright MW, Thompson DC, Silverman GA, Nebert DW and Vasiliou V.


book

Molecular basis of human hypertension: role of angiotensinogen
Cell 71 (1), 169-180 (1992)
Jeunemaitre X, Soubrier F, Kotelevtsev YV, Lifton RP, Williams CS, Charru A, Hunt SC, Hopkins PN, Williams RR and Lalouel JM.


book

Cloning, expression, and characterization of a gene encoding the human angiotensin II type 1A receptor
Biochem. Biophys. Res. Commun. 186 (1), 277-284 (1992)
Mauzy CA, Hwang O, Egloff AM, Wu LH and Chung FZ.


book

Purification and properties of prolylcarboxypeptidase (angiotensinase C) from human kidney
J. Biol. Chem. 253 (17), 5927-5931 (1978)
Odya,C.E., Marinkovic,D.V., Hammon,K.J., Stewart,T.A. and Erdos,E.G.


book

Post-proline cleaving enzyme. Purification of this endopeptidase by affinity chromatography
J. Biol. Chem. 251 (23), 7593-7599 (1976)
Koida,M. and Walter,R.


book

Angiotensin III: (DES-Aspartic Acid-1)-Angiotensin II. Evidence and speculation for its role as an important agonist in the renin - angiotensin system
Circ. Res. 36 (6 SUPPL 1), 38-48 (1975)
Goodfriend,T.L. and Peach,M.J.


 
*
*
*
*