E4F1 cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following E4F1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the E4F1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu27574 NM_004424.4
Latest version!
Homo sapiens E4F transcription factor 1 (E4F1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
OHu27640 NM_001288776.1
Latest version!
Homo sapiens E4F transcription factor 1 (E4F1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu06414 NM_001288778.1
Latest version!
Homo sapiens E4F transcription factor 1 (E4F1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu48775 XM_005255155.1
Latest version!
Homo sapiens E4F transcription factor 1 (E4F1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu48776 XM_005255156.1
Latest version!
Homo sapiens E4F transcription factor 1 (E4F1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu48778 XM_006720858.1
Latest version!
Homo sapiens E4F transcription factor 1 (E4F1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu57414 XM_011522402.1
Latest version!
Homo sapiens E4F transcription factor 1 (E4F1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu57415 XM_011522403.1
Latest version!
Homo sapiens E4F transcription factor 1 (E4F1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu107368 NM_004424.5
Latest version!
Homo sapiens E4F transcription factor 1 (E4F1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu85890 XM_017023010.1
Latest version!
Homo sapiens E4F transcription factor 1 (E4F1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu85891 XM_017023011.1
Latest version!
Homo sapiens E4F transcription factor 1 (E4F1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu27574
    Clone ID Related Accession (Same CDS sequence) NM_004424.4
    Accession Version NM_004424.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2355bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-02
    Organism Homo sapiens(human)
    Product transcription factor E4F1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY006534.1 and BC080524.1. On Jan 4, 2014 this sequence version replaced NM_004424.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC080524.1, U87269.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC
    GGCTTCCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGATGT GCACAGATGC
    GGCCGCTGCC AGGCAGAGTT CACCGCCTTG GAGGATTTTG TTCAGCACAA GATTCAGAAG
    GCCTGCCAGC GGGCCCCTCC GGAGGCCCTG CCTGCCACCC CTGCCACCAC AGCGTTGCTG
    GGCCAGGAGG TGGTGCCGGC AGCACCAGGC CCAGAGGAGC CCATCACTGT GGCCCACATC
    GTGGTGGAGG CGGCCTCTCT GGCAGCAGAC ATCAGCCACG CATCTGACCT TGTTGGTGGT
    GGGCACATCA AAGAGGTCAT CGTGGCTGCT GAGGCGGAGC TGGGAGACGG TGAGATGGCC
    GAGGCCCCGG GCAGCCCCCA CCAGCAGGGG CTGGGGCTCG CAGGGGAGGG TGAGCAGGCC
    CAGGTGAAGC TACTGGTGAA CAAGGATGGC CGCTATGTGT GTGCGCTGTG CCACAAGACC
    TTCAAGACGG GCAGCATCCT CAAGGCCCAC ATGGTCACTC ACAGCAGCCG CAAGGACCAC
    GAGTGCAAGC TCTGTGGGGC CTCCTTCCGC ACCAAGGGCT CACTCATCCG GCACCACCGG
    CGGCACACGG ATGAGCGCCC CTACAAGTGC TCCAAGTGTG GAAAGAGCTT CCGGGAGTCG
    GGTGCACTGA CCCGGCACCT CAAGTCTCTC ACCCCCTGCA CAGAGAAAAT CCGCTTCAGT
    GTGAGCAAGG ACGTGGTTGT CAGCAAAGAG GACGCACGTG CAGGTTCTGG AGCTGGAGCT
    GCCGGCTTGG GGACAGCCAC ATCATCGGTG ACAGGCGAGC CTATAGAGAC TTCACCCGTG
    ATTCACCTGG TGACAGATGC CAAGGGCACC GTCATCCACG AAGTCCACGT CCAGATGCAG
    GAGCTGTCCC TGGGCATGAA AGCCCTGGCC CCAGAGCCCC CCGTCTCCCA GGAGCTCCCC
    TGCTCCAGCG AGGGCAGCCG TGAGAACCTG CTGCACCAGG CCATGCAGAA CTCCGGCATC
    GTCCTTGAGC GCGCTGCTGG GGAGGAGGGT GCCCTGGAGC CAGCTCCTGC TGCCGGGTCC
    AGTCCCCAGC CCCTGGCAGT GGCAGCCCCG CAGCTGCCGG TACTGGAAGT GCAGCCGCTG
    GAGACACAGG TGGCCAGCGA GGCCTCAGCG GTGCCCAGGA CCCACCCATG TCCTCAGTGC
    AGTGAGACCT TCCCGACAGC AGCCACCCTG GAGGCCCACA AGAGGGGCCA CACCGGGCCG
    AGGCCGTTCG CCTGCGCGCA GTGTGGCAAG GCCTTCCCCA AGGCCTACCT GCTCAAGAAG
    CACCAGGAGG TGCACGTGCG TGAGCGCCGC TTCCGCTGTG GCGACTGCGG GAAGCTCTAC
    AAGACCATTG CCCATGTGCG TGGCCACCGG CGCGTCCACT CAGACGAGCG GCCCTACCCT
    TGTCCCAAGT GTGGCAAGCG CTACAAGACT AAGAACGCAC AGCAGGTGCA CTTCAGGACA
    CACCTGGAGG AGAAGCCGCA CGTGTGCCAG TTCTGCAGCC GTGGCTTCCG AGAGAAGGGC
    TCACTGGTGC GGCACGTGCG ACACCACACA GGCGAGAAGC CGTTCAAGTG CTACAAGTGC
    GGCCGTGGCT TCGCCGAGCA CGGCACGCTG AACCGGCACC TGCGCACCAA AGGGGGCTGC
    CTGCTGGAGG TGGAGGAGTT GCTGGTGTCT GAGGACAGCC CCGCGGCAGC CACCACCGTC
    CTCACGGAAG ACCCGCACAC AGTGTTGGTG GAGTTCTCGT CCGTGGTAGC TGACACCCAG
    GAGTATATCA TCGAGGCCAC TGCGGACGAT GCGGAGACCA GTGAGGCCAC GGAGATCATC
    GAGGGCACCC AGACAGAGGT GGACAGCCAC ATCATGAAGG TGGTGCAGCA GATCGTGCAC
    CAGGCTAGCG CCGGCCACCA GATCATCGTG CAGAACGTCA CCATGGACGA GGAGACGGCG
    CTGGGCCCAG AGGCGGCTGC CGCCGACACC ATCACCATCG CCACCCCCGA GAGCCTGACA
    GAGCAGGTGG CCATGACGCT GGCCTCGGCC ATCAGCGAGG GCACTGTGCT TGCCGCCCGG
    GCAGGGACAA GTGGCACTGA ACAGGCCACT GTGACCATGG TGTCATCAGA GGACATCGAG
    ATCCTGGAGC ATGCAGGCGA GCTGGTCATC GCCTCGCCGG AGGGCCAGCT GGAGGTGCAG
    ACGGTCATCG TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_004415.3
    CDS127..2481
    Translation

    Target ORF information:

    RefSeq Version NM_004424.4
    Organism Homo sapiens(human)
    Definition Homo sapiens E4F transcription factor 1 (E4F1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_004424.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC
    GGCTTCCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGATGT GCACAGATGC
    GGCCGCTGCC AGGCAGAGTT CACCGCCTTG GAGGATTTTG TTCAGCACAA GATTCAGAAG
    GCCTGCCAGC GGGCCCCTCC GGAGGCCCTG CCTGCCACCC CTGCCACCAC AGCGTTGCTG
    GGCCAGGAGG TGGTGCCGGC AGCACCAGGC CCAGAGGAGC CCATCACTGT GGCCCACATC
    GTGGTGGAGG CGGCCTCTCT GGCAGCAGAC ATCAGCCACG CATCTGACCT TGTTGGTGGT
    GGGCACATCA AAGAGGTCAT CGTGGCTGCT GAGGCGGAGC TGGGAGACGG TGAGATGGCC
    GAGGCCCCGG GCAGCCCCCA CCAGCAGGGG CTGGGGCTCG CAGGGGAGGG TGAGCAGGCC
    CAGGTGAAGC TACTGGTGAA CAAGGATGGC CGCTATGTGT GTGCGCTGTG CCACAAGACC
    TTCAAGACGG GCAGCATCCT CAAGGCCCAC ATGGTCACTC ACAGCAGCCG CAAGGACCAC
    GAGTGCAAGC TCTGTGGGGC CTCCTTCCGC ACCAAGGGCT CACTCATCCG GCACCACCGG
    CGGCACACGG ATGAGCGCCC CTACAAGTGC TCCAAGTGTG GAAAGAGCTT CCGGGAGTCG
    GGTGCACTGA CCCGGCACCT CAAGTCTCTC ACCCCCTGCA CAGAGAAAAT CCGCTTCAGT
    GTGAGCAAGG ACGTGGTTGT CAGCAAAGAG GACGCACGTG CAGGTTCTGG AGCTGGAGCT
    GCCGGCTTGG GGACAGCCAC ATCATCGGTG ACAGGCGAGC CTATAGAGAC TTCACCCGTG
    ATTCACCTGG TGACAGATGC CAAGGGCACC GTCATCCACG AAGTCCACGT CCAGATGCAG
    GAGCTGTCCC TGGGCATGAA AGCCCTGGCC CCAGAGCCCC CCGTCTCCCA GGAGCTCCCC
    TGCTCCAGCG AGGGCAGCCG TGAGAACCTG CTGCACCAGG CCATGCAGAA CTCCGGCATC
    GTCCTTGAGC GCGCTGCTGG GGAGGAGGGT GCCCTGGAGC CAGCTCCTGC TGCCGGGTCC
    AGTCCCCAGC CCCTGGCAGT GGCAGCCCCG CAGCTGCCGG TACTGGAAGT GCAGCCGCTG
    GAGACACAGG TGGCCAGCGA GGCCTCAGCG GTGCCCAGGA CCCACCCATG TCCTCAGTGC
    AGTGAGACCT TCCCGACAGC AGCCACCCTG GAGGCCCACA AGAGGGGCCA CACCGGGCCG
    AGGCCGTTCG CCTGCGCGCA GTGTGGCAAG GCCTTCCCCA AGGCCTACCT GCTCAAGAAG
    CACCAGGAGG TGCACGTGCG TGAGCGCCGC TTCCGCTGTG GCGACTGCGG GAAGCTCTAC
    AAGACCATTG CCCATGTGCG TGGCCACCGG CGCGTCCACT CAGACGAGCG GCCCTACCCT
    TGTCCCAAGT GTGGCAAGCG CTACAAGACT AAGAACGCAC AGCAGGTGCA CTTCAGGACA
    CACCTGGAGG AGAAGCCGCA CGTGTGCCAG TTCTGCAGCC GTGGCTTCCG AGAGAAGGGC
    TCACTGGTGC GGCACGTGCG ACACCACACA GGCGAGAAGC CGTTCAAGTG CTACAAGTGC
    GGCCGTGGCT TCGCCGAGCA CGGCACGCTG AACCGGCACC TGCGCACCAA AGGGGGCTGC
    CTGCTGGAGG TGGAGGAGTT GCTGGTGTCT GAGGACAGCC CCGCGGCAGC CACCACCGTC
    CTCACGGAAG ACCCGCACAC AGTGTTGGTG GAGTTCTCGT CCGTGGTAGC TGACACCCAG
    GAGTATATCA TCGAGGCCAC TGCGGACGAT GCGGAGACCA GTGAGGCCAC GGAGATCATC
    GAGGGCACCC AGACAGAGGT GGACAGCCAC ATCATGAAGG TGGTGCAGCA GATCGTGCAC
    CAGGCTAGCG CCGGCCACCA GATCATCGTG CAGAACGTCA CCATGGACGA GGAGACGGCG
    CTGGGCCCAG AGGCGGCTGC CGCCGACACC ATCACCATCG CCACCCCCGA GAGCCTGACA
    GAGCAGGTGG CCATGACGCT GGCCTCGGCC ATCAGCGAGG GCACTGTGCT TGCCGCCCGG
    GCAGGGACAA GTGGCACTGA ACAGGCCACT GTGACCATGG TGTCATCAGA GGACATCGAG
    ATCCTGGAGC ATGCAGGCGA GCTGGTCATC GCCTCGCCGG AGGGCCAGCT GGAGGTGCAG
    ACGGTCATCG TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27640
    Clone ID Related Accession (Same CDS sequence) NM_001288776.1
    Accession Version NM_001288776.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1965bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-24
    Organism Homo sapiens(human)
    Product transcription factor E4F1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY006534.1, BC014068.2 and BC080524.1. Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region that results in a frameshift compared to variant 1. The encoded protein (isoform 2) has a shorter and distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC014068.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2155628, SAMN01820687 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC
    GGCTTCCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGATGT GCACAGATGC
    GGCCGCTGCC AGGCAGAGTT CACCGCCTTG GAGGATTTTG TTCAGCACAA GATTCAGAAG
    GCCTGCCAGC GGGCCCCTCC GGAGGCCCTG CCTGCCACCC CTGCCACCAC AGCGTTGCTG
    GGCCAGGAGG TGGTGCCGGC AGCACCAGGC CCAGAGGAGC CCATCACTGT GGCCCACATC
    GTGGTGGAGG CGGCCTCTCT GGCAGCAGAC ATCAGCCACG CATCTGACCT TGTTGGTGGT
    GGGCACATCA AAGAGGTCAT CGTGGCTGCT GAGGCGGAGC TGGGAGACGG TGAGATGGCC
    GAGGCCCCGG GCAGCCCCCA CCAGCAGGGG CTGGGGCTCG CAGGGGAGGG TGAGCAGGCC
    CAGGTGAAGC TACTGGTGAA CAAGGATGGC CGCTATGTGT GTGCGCTGTG CCACAAGACC
    TTCAAGACGG GCAGCATCCT CAAGGCCCAC ATGGTCACTC ACAGCAGCCG CAAGGACCAC
    GAGTGCAAGC TCTGTGGGGC CTCCTTCCGC ACCAAGGGCT CACTCATCCG GCACCACCGG
    CGGCACACGG ATGAGCGCCC CTACAAGTGC TCCAAGTGTG GAAAGAGCTT CCGGGAGTCG
    GGTGCACTGA CCCGGCACCT CAAGTCTCTC ACCCCCTGCA CAGAGAAAAT CCGCTTCAGT
    GTGAGCAAGG ACGTGGTTGT CAGCAAAGAG GACGCACGTG CAGGTTCTGG AGCTGGAGCT
    GCCGGCTTGG GGACAGCCAC ATCATCGGTG ACAGGCGAGC CTATAGAGAC TTCACCCGTG
    ATTCACCTGG TGACAGATGC CAAGGGCACC GTCATCCACG AAGTCCACGT CCAGATGCAG
    GAGCTGTCCC TGGGCATGAA AGCCCTGGCC CCAGAGCCCC CCGTCTCCCA GGAGCTCCCC
    TGCTCCAGCG AGGGCAGCCG TGAGAACCTG CTGCACCAGG CCATGCAGAA CTCCGGCATC
    GTCCTTGAGC GCGCTGCTGG GGAGGAGGGT GCCCTGGAGC CAGCTCCTGC TGCCGGGTCC
    AGTCCCCAGC CCCTGGCAGT GGCAGCCCCG CAGCTGCCGG TACTGGAAGT GCAGCCGCTG
    GAGACACAGG TGGCCAGCGA GGCCTCAGCG GTGCCCAGGA CCCACCCATG TCCTCAGTGC
    AGTGAGACCT TCCCGACAGC AGCCACCCTG GAGGCCCACA AGAGGGGCCA CACCGGGCCG
    AGGCCGTTCG CCTGCGCGCA GTGTGGCAAG GCCTTCCCCA AGGCCTACCT GCTCAAGAAG
    CACCAGGAGG TGCACGTGCG TGAGCGCCGC TTCCGCTGTG GCGACTGCGG GAAGCTCTAC
    AAGACCATTG CCCATGTGCG TGGCCACCGG CGCGTCCACT CAGACGAGCG GCCCTACCCT
    TGTCCCAAGT GTGGCAAGCG CTACAAGACT AAGAACGCAC AGCAGGTGCA CTTCAGGACA
    CACCTGGAGG AGAAGCCGCA CGTGTGCCAG TTCTGCAGCC GTGGCTTCCG AGAGAAGGGC
    TCACTGGTGC GGCACGTGCG ACACCACACA GGCGAGAAGC CGTTCAAGTG CTACAAGTGC
    GGCCGTGGCT TCGCCGAGCA CGGCACGCTG AACCGGCACC TGCGCACCAA AGGGGGCTGC
    CTGCTGGAGG TGGAGGAGTT GCTGGTGTCT GAGGACAGCC CCGCGGCAGC CACCACCGTC
    CTCACGGAAG ACCCGCACAC AGTGCCACTG CGGACGATGC GGAGACCAGT GAGGCCACGG
    AGATCATCGA GGGCACCCAG ACAGAGGTGG ACAGCCACAT CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001275705.1
    CDS127..2091
    Translation

    Target ORF information:

    RefSeq Version NM_001288776.1
    Organism Homo sapiens(human)
    Definition Homo sapiens E4F transcription factor 1 (E4F1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001288776.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC
    GGCTTCCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGATGT GCACAGATGC
    GGCCGCTGCC AGGCAGAGTT CACCGCCTTG GAGGATTTTG TTCAGCACAA GATTCAGAAG
    GCCTGCCAGC GGGCCCCTCC GGAGGCCCTG CCTGCCACCC CTGCCACCAC AGCGTTGCTG
    GGCCAGGAGG TGGTGCCGGC AGCACCAGGC CCAGAGGAGC CCATCACTGT GGCCCACATC
    GTGGTGGAGG CGGCCTCTCT GGCAGCAGAC ATCAGCCACG CATCTGACCT TGTTGGTGGT
    GGGCACATCA AAGAGGTCAT CGTGGCTGCT GAGGCGGAGC TGGGAGACGG TGAGATGGCC
    GAGGCCCCGG GCAGCCCCCA CCAGCAGGGG CTGGGGCTCG CAGGGGAGGG TGAGCAGGCC
    CAGGTGAAGC TACTGGTGAA CAAGGATGGC CGCTATGTGT GTGCGCTGTG CCACAAGACC
    TTCAAGACGG GCAGCATCCT CAAGGCCCAC ATGGTCACTC ACAGCAGCCG CAAGGACCAC
    GAGTGCAAGC TCTGTGGGGC CTCCTTCCGC ACCAAGGGCT CACTCATCCG GCACCACCGG
    CGGCACACGG ATGAGCGCCC CTACAAGTGC TCCAAGTGTG GAAAGAGCTT CCGGGAGTCG
    GGTGCACTGA CCCGGCACCT CAAGTCTCTC ACCCCCTGCA CAGAGAAAAT CCGCTTCAGT
    GTGAGCAAGG ACGTGGTTGT CAGCAAAGAG GACGCACGTG CAGGTTCTGG AGCTGGAGCT
    GCCGGCTTGG GGACAGCCAC ATCATCGGTG ACAGGCGAGC CTATAGAGAC TTCACCCGTG
    ATTCACCTGG TGACAGATGC CAAGGGCACC GTCATCCACG AAGTCCACGT CCAGATGCAG
    GAGCTGTCCC TGGGCATGAA AGCCCTGGCC CCAGAGCCCC CCGTCTCCCA GGAGCTCCCC
    TGCTCCAGCG AGGGCAGCCG TGAGAACCTG CTGCACCAGG CCATGCAGAA CTCCGGCATC
    GTCCTTGAGC GCGCTGCTGG GGAGGAGGGT GCCCTGGAGC CAGCTCCTGC TGCCGGGTCC
    AGTCCCCAGC CCCTGGCAGT GGCAGCCCCG CAGCTGCCGG TACTGGAAGT GCAGCCGCTG
    GAGACACAGG TGGCCAGCGA GGCCTCAGCG GTGCCCAGGA CCCACCCATG TCCTCAGTGC
    AGTGAGACCT TCCCGACAGC AGCCACCCTG GAGGCCCACA AGAGGGGCCA CACCGGGCCG
    AGGCCGTTCG CCTGCGCGCA GTGTGGCAAG GCCTTCCCCA AGGCCTACCT GCTCAAGAAG
    CACCAGGAGG TGCACGTGCG TGAGCGCCGC TTCCGCTGTG GCGACTGCGG GAAGCTCTAC
    AAGACCATTG CCCATGTGCG TGGCCACCGG CGCGTCCACT CAGACGAGCG GCCCTACCCT
    TGTCCCAAGT GTGGCAAGCG CTACAAGACT AAGAACGCAC AGCAGGTGCA CTTCAGGACA
    CACCTGGAGG AGAAGCCGCA CGTGTGCCAG TTCTGCAGCC GTGGCTTCCG AGAGAAGGGC
    TCACTGGTGC GGCACGTGCG ACACCACACA GGCGAGAAGC CGTTCAAGTG CTACAAGTGC
    GGCCGTGGCT TCGCCGAGCA CGGCACGCTG AACCGGCACC TGCGCACCAA AGGGGGCTGC
    CTGCTGGAGG TGGAGGAGTT GCTGGTGTCT GAGGACAGCC CCGCGGCAGC CACCACCGTC
    CTCACGGAAG ACCCGCACAC AGTGCCACTG CGGACGATGC GGAGACCAGT GAGGCCACGG
    AGATCATCGA GGGCACCCAG ACAGAGGTGG ACAGCCACAT CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu06414
    Clone ID Related Accession (Same CDS sequence) NM_001288778.1
    Accession Version NM_001288778.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1824bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-24
    Organism Homo sapiens(human)
    Product transcription factor E4F1 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY006534.1, AK225132.1, DN998026.1 and BC080524.1. Transcript Variant: This variant (3) lacks two alternate in-frame exons and has two alternate in-frame splice sites in the 3' coding region, compared to variant 1. The encoded protein (isoform 3) is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK225132.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC
    GGCTTCCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGATGT GCACAGATGC
    GGCCGCTGCC AGGCAGAGTT CACCGCCTTG GAGGATTTTG TTCAGCACAA GATTCAGAAG
    GCCTGCCAGC GGGCCCCTCC GGAGGCCCTG CCTGCCACCC CTGCCACCAC AGCGTTGCTG
    GGCCAGGAGG TGGTGCCGGC AGCACCAGGC CCAGAGGAGC CCATCACTGT GGCCCACATC
    GTGGTGGAGG CGGCCTCTCT GGCAGCAGAC ATCAGCCACG CATCTGACCT TGTTGGTGGT
    GGGCACATCA AAGAGGTCAT CGTGGCTGCT GAGGCGGAGC TGGGAGACGG TGAGATGGCC
    GAGGCCCCGG GCAGCCCCCA CCAGCAGGGG CTGGGGCTCG CAGGGGAGGG TGAGCAGGCC
    CAGGTGAAGC TACTGGTGAA CAAGGATGGC CGCTATGTGT GTGCGCTGTG CCACAAGACC
    TTCAAGACGG GCAGCATCCT CAAGGCCCAC ATGGTCACTC ACAGCAGCCG CAAGGACCAC
    GAGTGCAAGC TCTGTGGGGC CTCCTTCCGC ACCAAGGGCT CACTCATCCG GCACCACCGG
    CGGCACACGG ATGAGCGCCC CTACAAGTGC TCCAAGTGTG GAAAGAGCTT CCGGGAGTCG
    GGTGCACTGA CCCGGCACCT CAAGTCTCTC ACCCCCTGCA CAGAGAAAAT CCGCTTCAGT
    GTGAGCAAGG ACGTGGTTGT CAGCAAAGAG GACGCACGTG CAGGTTCTGG AGCTGGAGCT
    GCCGGCTTGG GGACAGCCAC ATCATCGGTG ACAGGCGAGC CTATAGAGAC TTCACCCGTG
    ATTCACCTGG TGACAGATGC CAAGGGCACC GTCATCCACG AAGTCCACGT CCAGATGCAG
    GAGCTGTCCC TGGGCATGAA AGCCCTGGCC CCAGAGCCCC CCGTCTCCCA GGAGCTCCCC
    TGCTCCAGCG AGGGCAGCCG TGAGAACCTG CTGCACCAGT TCTGCAGCCG TGGCTTCCGA
    GAGAAGGGCT CACTGGTGCG GCACGTGCGA CACCACACAG GCGAGAAGCC GTTCAAGTGC
    TACAAGTGCG GCCGTGGCTT CGCCGAGCAC GGCACGCTGA ACCGGCACCT GCGCACCAAA
    GGGGGCTGCC TGCTGGAGGT GGAGGAGTTG CTGGTGTCTG AGGACAGCCC CGCGGCAGCC
    ACCACCGTCC TCACGGAAGA CCCGCACACA GTGTTGGTGG AGTTCTCGTC CGTGGTAGCT
    GACACCCAGG AGTATATCAT CGAGGCCACT GCGGACGATG CGGAGACCAG TGAGGCCACG
    GAGATCATCG AGGGCACCCA GACAGAGGTG GACAGCCACA TCATGAAGGT GGTGCAGCAG
    ATCGTGCACC AGGCTAGCGC CGGCCACCAG ATCATCGTGC AGAACGTCAC CATGGACGAG
    GAGACGGCGC TGGGCCCAGA GGCGGCTGCC GCCGACACCA TCACCATCGC CACCCCCGAG
    AGCCTGACAG AGCAGGTGGC CATGACGCTG GCCTCGGCCA TCAGCGAGGG CACTGTGCTT
    GCCGCCCGGG CAGGGACAAG TGGCACTGAA CAGGCCACTG TGACCATGGT GTCATCAGAG
    GACATCGAGA TCCTGGAGCA TGCAGGCGAG CTGGTCATCG CCTCGCCGGA GGGCCAGCTG
    GAGGTGCAGA CGGTCATCGT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001275707.1
    CDS127..1950
    Translation

    Target ORF information:

    RefSeq Version NM_001288778.1
    Organism Homo sapiens(human)
    Definition Homo sapiens E4F transcription factor 1 (E4F1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001288778.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC
    GGCTTCCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGATGT GCACAGATGC
    GGCCGCTGCC AGGCAGAGTT CACCGCCTTG GAGGATTTTG TTCAGCACAA GATTCAGAAG
    GCCTGCCAGC GGGCCCCTCC GGAGGCCCTG CCTGCCACCC CTGCCACCAC AGCGTTGCTG
    GGCCAGGAGG TGGTGCCGGC AGCACCAGGC CCAGAGGAGC CCATCACTGT GGCCCACATC
    GTGGTGGAGG CGGCCTCTCT GGCAGCAGAC ATCAGCCACG CATCTGACCT TGTTGGTGGT
    GGGCACATCA AAGAGGTCAT CGTGGCTGCT GAGGCGGAGC TGGGAGACGG TGAGATGGCC
    GAGGCCCCGG GCAGCCCCCA CCAGCAGGGG CTGGGGCTCG CAGGGGAGGG TGAGCAGGCC
    CAGGTGAAGC TACTGGTGAA CAAGGATGGC CGCTATGTGT GTGCGCTGTG CCACAAGACC
    TTCAAGACGG GCAGCATCCT CAAGGCCCAC ATGGTCACTC ACAGCAGCCG CAAGGACCAC
    GAGTGCAAGC TCTGTGGGGC CTCCTTCCGC ACCAAGGGCT CACTCATCCG GCACCACCGG
    CGGCACACGG ATGAGCGCCC CTACAAGTGC TCCAAGTGTG GAAAGAGCTT CCGGGAGTCG
    GGTGCACTGA CCCGGCACCT CAAGTCTCTC ACCCCCTGCA CAGAGAAAAT CCGCTTCAGT
    GTGAGCAAGG ACGTGGTTGT CAGCAAAGAG GACGCACGTG CAGGTTCTGG AGCTGGAGCT
    GCCGGCTTGG GGACAGCCAC ATCATCGGTG ACAGGCGAGC CTATAGAGAC TTCACCCGTG
    ATTCACCTGG TGACAGATGC CAAGGGCACC GTCATCCACG AAGTCCACGT CCAGATGCAG
    GAGCTGTCCC TGGGCATGAA AGCCCTGGCC CCAGAGCCCC CCGTCTCCCA GGAGCTCCCC
    TGCTCCAGCG AGGGCAGCCG TGAGAACCTG CTGCACCAGT TCTGCAGCCG TGGCTTCCGA
    GAGAAGGGCT CACTGGTGCG GCACGTGCGA CACCACACAG GCGAGAAGCC GTTCAAGTGC
    TACAAGTGCG GCCGTGGCTT CGCCGAGCAC GGCACGCTGA ACCGGCACCT GCGCACCAAA
    GGGGGCTGCC TGCTGGAGGT GGAGGAGTTG CTGGTGTCTG AGGACAGCCC CGCGGCAGCC
    ACCACCGTCC TCACGGAAGA CCCGCACACA GTGTTGGTGG AGTTCTCGTC CGTGGTAGCT
    GACACCCAGG AGTATATCAT CGAGGCCACT GCGGACGATG CGGAGACCAG TGAGGCCACG
    GAGATCATCG AGGGCACCCA GACAGAGGTG GACAGCCACA TCATGAAGGT GGTGCAGCAG
    ATCGTGCACC AGGCTAGCGC CGGCCACCAG ATCATCGTGC AGAACGTCAC CATGGACGAG
    GAGACGGCGC TGGGCCCAGA GGCGGCTGCC GCCGACACCA TCACCATCGC CACCCCCGAG
    AGCCTGACAG AGCAGGTGGC CATGACGCTG GCCTCGGCCA TCAGCGAGGG CACTGTGCTT
    GCCGCCCGGG CAGGGACAAG TGGCACTGAA CAGGCCACTG TGACCATGGT GTCATCAGAG
    GACATCGAGA TCCTGGAGCA TGCAGGCGAG CTGGTCATCG CCTCGCCGGA GGGCCAGCTG
    GAGGTGCAGA CGGTCATCGT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu48775
    Clone ID Related Accession (Same CDS sequence) XM_005255155.1
    Accession Version XM_005255155.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2514bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product transcription factor E4F1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC
    GGCTTCCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGGTA ACCGGGCCGA CCCGGAGGGT
    GCGGCCGGGG TGCGGGCAGT TCATCCCGGG CTGGCAGAGG CCCGGATGGC CCGAGCTGCG
    GGCTCGACCG ACCCTCCCAG ACCCAGACAC CTCGCGGATC TCGCGGGACC CTCACAGCCC
    TCCACGAAAC CCCCAGATGA GGACGATGTG CACAGATGCG GCCGCTGCCA GGCAGAGTTC
    ACCGCCTTGG AGGATTTTGT TCAGCACAAG ATTCAGAAGG CCTGCCAGCG GGCCCCTCCG
    GAGGCCCTGC CTGCCACCCC TGCCACCACA GCGTTGCTGG GCCAGGAGGT GGTGCCGGCA
    GCACCAGGCC CAGAGGAGCC CATCACTGTG GCCCACATCG TGGTGGAGGC GGCCTCTCTG
    GCAGCAGACA TCAGCCACGC ATCTGACCTT GTTGGTGGTG GGCACATCAA AGAGGTCATC
    GTGGCTGCTG AGGCGGAGCT GGGAGACGGT GAGATGGCCG AGGCCCCGGG CAGCCCCCGC
    CAGCAGGGGC TGGGGCTCGC AGGGGAGGGT GAGCAGGCCC AGGTGAAGCT ACTGGTGAAC
    AAGGATGGCC GCTATGTGTG TGCGCTGTGC CACAAGACCT TCAAGACGGG CAGCATCCTC
    AAGGCCCACA TGGTCACTCA CAGCAGCCGC AAGGACCACG AGTGCAAGCT CTGTGGGGCC
    TCCTTCCGCA CCAAGGGCTC ACTCATCCGG CACCACCGGC GGCACACGGA TGAGCGCCCC
    TACAAGTGCT CCAAGTGTGG AAAGAGCTTC CGGGAGTCGG GTGCACTGAC CCGGCACCTC
    AAGTCTCTCA CCCCCTGCAC AGAGAAAATC CGCTTCAGTG TGAGCAAGGA CGTGGTTGTC
    AGCAAAGAGG ACGCACGTGC AGGTTCTGGA GCTGGAGCTG CCGGCTTGGG GACAGCCACA
    TCATCGGTGA CAGGCGAGCC TATAGAGACT TCACCCGTGA TTCACCTGGT GACAGATGCC
    AAGGGCACCG TCATCCACGA AGTCCACGTC CAGATGCAGG AGCTGTCCCT GGGCATGAAA
    GCCCTGGCCC CAGAGCCCCC CGTCTCCCAG GAGCTCCCCT GCTCCAGCGA GGGCAGCCGT
    GAGAACCTGC TGCACCAGGC CATGCAGAAC TCCGGCATCG TCCTTGAGCG CGCTGCTGGG
    GAGGAGGGTG CCCTGGAGCC AGCTCCTGCT GCCGGGTCCA GTCCCCAGCC CCTGGCAGTG
    GCAGCCCCGC AGCTGCCGGT ACTGGAAGTG CAGCCGCTGG AGACACAGGT GGCCAGCGAG
    GCCTCAGCGG TGCCCAGGAC CCACCCATGT CCTCAGTGCA GTGAGACCTT CCCGACAGCA
    GCCACCCTGG AGGCCCACAA GAGGGGCCAC ACCGGGCCGA GGCCGTTCGC CTGCGCGCAG
    TGTGGCAAGG CCTTCCCCAA GGCCTACCTG CTCAAGAAGC ACCAGGAGGT GCACGTGCGT
    GAGCGCCGCT TCCGCTGTGG CGACTGCGGG AAGCTCTACA AGACCATTGC CCATGTGCGT
    GGCCACCGGC GCGTCCACTC AGACGAGCGG CCCTACCCTT GTCCCAAGTG TGGCAAGCGC
    TACAAGACTA AGAACGCACA GCAGGTGCAC TTCAGGACAC ACCTGGAGGA GAAGCCGCAC
    GTGTGCCAGT TCTGCAGCCG TGGCTTCCGA GAGAAGGGCT CACTGGTGCG GCACGTGCGA
    CACCACACAG GCGAGAAGCC GTTCAAGTGC TACAAGTGCG GCCGTGGCTT CGCCGAGCAC
    GGCACGCTGA ACCGGCACCT GCGCACCAAA GGGGGCTGCC TGCTGGAGGT GGAGGAGTTG
    CTGGTGTCTG AGGACAGCCC CGCGGCAGCC ACCACCGTCC TCACGGAAGA CCCGCACACA
    GTGTTGGTGG AGTTCTCGTC CGTGGTAGCT GACACCCAGG AGTATATCAT CGAGGCCACT
    GCGGACGATG CGGAGACCAG TGAGGCCACG GAGATCATCG AGGGCACCCA GACAGAGGTG
    GACAGCCACA TCATGAAGGT GGTGCAGCAG ATCGTGCACC AGGCTAGCGC CGGCCACCAG
    ATCATCGTGC AGAACGTCAC CATGGACGAG GAGACGGCGC TGGGCCCAGA GGCGGCTGCC
    GCCGACACCA TCACCATCGC CACCCCCGAG AGCCTGACAG AGCAGGTGGC CATGACGCTG
    GCCTCGGCCA TCAGCGAGGG CACTGTGCTT GCCGCCCGGG CAGGGACAAG TGGCACTGAA
    CAGGCCACTG TGACCATGGT GTCATCAGAG GACATCGAGA TCCTGGAGCA TGCAGGCGAG
    CTGGTCATCG CCTCGCCGGA GGGCCAGCTG GAGGTGCAGA CGGTCATCGT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005255212.1
    CDS117..2630
    Translation

    Target ORF information:

    RefSeq Version XM_005255155.1
    Organism Homo sapiens(human)
    Definition Homo sapiens E4F transcription factor 1 (E4F1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005255155.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC
    GGCTTCCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGGTA ACCGGGCCGA CCCGGAGGGT
    GCGGCCGGGG TGCGGGCAGT TCATCCCGGG CTGGCAGAGG CCCGGATGGC CCGAGCTGCG
    GGCTCGACCG ACCCTCCCAG ACCCAGACAC CTCGCGGATC TCGCGGGACC CTCACAGCCC
    TCCACGAAAC CCCCAGATGA GGACGATGTG CACAGATGCG GCCGCTGCCA GGCAGAGTTC
    ACCGCCTTGG AGGATTTTGT TCAGCACAAG ATTCAGAAGG CCTGCCAGCG GGCCCCTCCG
    GAGGCCCTGC CTGCCACCCC TGCCACCACA GCGTTGCTGG GCCAGGAGGT GGTGCCGGCA
    GCACCAGGCC CAGAGGAGCC CATCACTGTG GCCCACATCG TGGTGGAGGC GGCCTCTCTG
    GCAGCAGACA TCAGCCACGC ATCTGACCTT GTTGGTGGTG GGCACATCAA AGAGGTCATC
    GTGGCTGCTG AGGCGGAGCT GGGAGACGGT GAGATGGCCG AGGCCCCGGG CAGCCCCCGC
    CAGCAGGGGC TGGGGCTCGC AGGGGAGGGT GAGCAGGCCC AGGTGAAGCT ACTGGTGAAC
    AAGGATGGCC GCTATGTGTG TGCGCTGTGC CACAAGACCT TCAAGACGGG CAGCATCCTC
    AAGGCCCACA TGGTCACTCA CAGCAGCCGC AAGGACCACG AGTGCAAGCT CTGTGGGGCC
    TCCTTCCGCA CCAAGGGCTC ACTCATCCGG CACCACCGGC GGCACACGGA TGAGCGCCCC
    TACAAGTGCT CCAAGTGTGG AAAGAGCTTC CGGGAGTCGG GTGCACTGAC CCGGCACCTC
    AAGTCTCTCA CCCCCTGCAC AGAGAAAATC CGCTTCAGTG TGAGCAAGGA CGTGGTTGTC
    AGCAAAGAGG ACGCACGTGC AGGTTCTGGA GCTGGAGCTG CCGGCTTGGG GACAGCCACA
    TCATCGGTGA CAGGCGAGCC TATAGAGACT TCACCCGTGA TTCACCTGGT GACAGATGCC
    AAGGGCACCG TCATCCACGA AGTCCACGTC CAGATGCAGG AGCTGTCCCT GGGCATGAAA
    GCCCTGGCCC CAGAGCCCCC CGTCTCCCAG GAGCTCCCCT GCTCCAGCGA GGGCAGCCGT
    GAGAACCTGC TGCACCAGGC CATGCAGAAC TCCGGCATCG TCCTTGAGCG CGCTGCTGGG
    GAGGAGGGTG CCCTGGAGCC AGCTCCTGCT GCCGGGTCCA GTCCCCAGCC CCTGGCAGTG
    GCAGCCCCGC AGCTGCCGGT ACTGGAAGTG CAGCCGCTGG AGACACAGGT GGCCAGCGAG
    GCCTCAGCGG TGCCCAGGAC CCACCCATGT CCTCAGTGCA GTGAGACCTT CCCGACAGCA
    GCCACCCTGG AGGCCCACAA GAGGGGCCAC ACCGGGCCGA GGCCGTTCGC CTGCGCGCAG
    TGTGGCAAGG CCTTCCCCAA GGCCTACCTG CTCAAGAAGC ACCAGGAGGT GCACGTGCGT
    GAGCGCCGCT TCCGCTGTGG CGACTGCGGG AAGCTCTACA AGACCATTGC CCATGTGCGT
    GGCCACCGGC GCGTCCACTC AGACGAGCGG CCCTACCCTT GTCCCAAGTG TGGCAAGCGC
    TACAAGACTA AGAACGCACA GCAGGTGCAC TTCAGGACAC ACCTGGAGGA GAAGCCGCAC
    GTGTGCCAGT TCTGCAGCCG TGGCTTCCGA GAGAAGGGCT CACTGGTGCG GCACGTGCGA
    CACCACACAG GCGAGAAGCC GTTCAAGTGC TACAAGTGCG GCCGTGGCTT CGCCGAGCAC
    GGCACGCTGA ACCGGCACCT GCGCACCAAA GGGGGCTGCC TGCTGGAGGT GGAGGAGTTG
    CTGGTGTCTG AGGACAGCCC CGCGGCAGCC ACCACCGTCC TCACGGAAGA CCCGCACACA
    GTGTTGGTGG AGTTCTCGTC CGTGGTAGCT GACACCCAGG AGTATATCAT CGAGGCCACT
    GCGGACGATG CGGAGACCAG TGAGGCCACG GAGATCATCG AGGGCACCCA GACAGAGGTG
    GACAGCCACA TCATGAAGGT GGTGCAGCAG ATCGTGCACC AGGCTAGCGC CGGCCACCAG
    ATCATCGTGC AGAACGTCAC CATGGACGAG GAGACGGCGC TGGGCCCAGA GGCGGCTGCC
    GCCGACACCA TCACCATCGC CACCCCCGAG AGCCTGACAG AGCAGGTGGC CATGACGCTG
    GCCTCGGCCA TCAGCGAGGG CACTGTGCTT GCCGCCCGGG CAGGGACAAG TGGCACTGAA
    CAGGCCACTG TGACCATGGT GTCATCAGAG GACATCGAGA TCCTGGAGCA TGCAGGCGAG
    CTGGTCATCG CCTCGCCGGA GGGCCAGCTG GAGGTGCAGA CGGTCATCGT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu48776
    Clone ID Related Accession (Same CDS sequence) XM_005255156.1
    Accession Version XM_005255156.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2511bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product transcription factor E4F1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC
    GGCTTCCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGGTA ACCGGGCCGA CCCGGAGGGT
    GCGGCCGGGG TGCGGGCAGT TCATCCCGGG CTGGCAGAGG CCCGGATGGC CCGAGCTGCG
    GGCTCGACCG ACCCTCCCAG ACCCAGACAC CTCGCGGATC TCGCGGGACC CTCACAGCCC
    TCCACGAAAC CCCCAGATGA GGACGATGTG CACAGATGCG GCCGCTGCCA GGCAGAGTTC
    ACCGCCTTGG AGGATTTTGT TCAGCACAAG ATTCAGAAGG CCTGCCAGCG GGCCCCTCCG
    GAGGCCCTGC CTGCCACCCC TGCCACCACA GCGTTGCTGG GCCAGGAGGT GGTGCCGGCA
    GCACCAGGCC CAGAGGAGCC CATCACTGTG GCCCACATCG TGGTGGAGGC GGCCTCTCTG
    GCAGCAGACA TCAGCCACGC ATCTGACCTT GTTGGTGGTG GGCACATCAA AGAGGTCATC
    GTGGCTGCTG AGGCGGAGCT GGGAGACGGT GAGATGGCCG AGGCCCCGGG CAGCCCCCGC
    CAGCAGGGGC TGGGGCTCGC AGGGGAGGGT GAGCAGGCCC AGGTGAAGCT ACTGGTGAAC
    AAGGATGGCC GCTATGTGTG TGCGCTGTGC CACAAGACCT TCAAGACGGG CAGCATCCTC
    AAGGCCCACA TGGTCACTCA CAGCAGCCGC AAGGACCACG AGTGCAAGCT CTGTGGGGCC
    TCCTTCCGCA CCAAGGGCTC ACTCATCCGG CACCACCGGC GGCACACGGA TGAGCGCCCC
    TACAAGTGCT CCAAGTGTGG AAAGAGCTTC CGGGAGTCGG GTGCACTGAC CCGGCACCTC
    AAGTCTCTCA CCCCCTGCAC AGAGAAAATC CGCTTCAGTG TGAGCAAGGA CGTGGTTGTC
    AGCAAAGAGG ACGCACGTGC AGGTTCTGGA GCTGGAGCTG CCGGCTTGGG GACAGCCACA
    TCATCGGTGA CAGGCGAGCC TATAGAGACT TCACCCGTGA TTCACCTGGT GACAGATGCC
    AAGGGCACCG TCATCCACGA AGTCCACGTC CAGATGCAGG AGCTGTCCCT GGGCATGAAA
    GCCCTGGCCC CAGAGCCCCC CGTCTCCCAG GAGCTCCCCT GCTCCAGCGA GGGCAGCCGT
    GAGAACCTGC TGCACCAGGC CATGCAGAAC TCCGGCATCG TCCTTGAGCG CGCTGCTGGG
    GAGGAGGGTG CCCTGGAGCC AGCTCCTGCT GCCGGGTCCA GTCCCCAGCC CCTGGCAGTG
    GCAGCCCCGC AGCTGCCGGT ACTGGAAGTG CAGCCGCTGG AGACAGTGGC CAGCGAGGCC
    TCAGCGGTGC CCAGGACCCA CCCATGTCCT CAGTGCAGTG AGACCTTCCC GACAGCAGCC
    ACCCTGGAGG CCCACAAGAG GGGCCACACC GGGCCGAGGC CGTTCGCCTG CGCGCAGTGT
    GGCAAGGCCT TCCCCAAGGC CTACCTGCTC AAGAAGCACC AGGAGGTGCA CGTGCGTGAG
    CGCCGCTTCC GCTGTGGCGA CTGCGGGAAG CTCTACAAGA CCATTGCCCA TGTGCGTGGC
    CACCGGCGCG TCCACTCAGA CGAGCGGCCC TACCCTTGTC CCAAGTGTGG CAAGCGCTAC
    AAGACTAAGA ACGCACAGCA GGTGCACTTC AGGACACACC TGGAGGAGAA GCCGCACGTG
    TGCCAGTTCT GCAGCCGTGG CTTCCGAGAG AAGGGCTCAC TGGTGCGGCA CGTGCGACAC
    CACACAGGCG AGAAGCCGTT CAAGTGCTAC AAGTGCGGCC GTGGCTTCGC CGAGCACGGC
    ACGCTGAACC GGCACCTGCG CACCAAAGGG GGCTGCCTGC TGGAGGTGGA GGAGTTGCTG
    GTGTCTGAGG ACAGCCCCGC GGCAGCCACC ACCGTCCTCA CGGAAGACCC GCACACAGTG
    TTGGTGGAGT TCTCGTCCGT GGTAGCTGAC ACCCAGGAGT ATATCATCGA GGCCACTGCG
    GACGATGCGG AGACCAGTGA GGCCACGGAG ATCATCGAGG GCACCCAGAC AGAGGTGGAC
    AGCCACATCA TGAAGGTGGT GCAGCAGATC GTGCACCAGG CTAGCGCCGG CCACCAGATC
    ATCGTGCAGA ACGTCACCAT GGACGAGGAG ACGGCGCTGG GCCCAGAGGC GGCTGCCGCC
    GACACCATCA CCATCGCCAC CCCCGAGAGC CTGACAGAGC AGGTGGCCAT GACGCTGGCC
    TCGGCCATCA GCGAGGGCAC TGTGCTTGCC GCCCGGGCAG GGACAAGTGG CACTGAACAG
    GCCACTGTGA CCATGGTGTC ATCAGAGGAC ATCGAGATCC TGGAGCATGC AGGCGAGCTG
    GTCATCGCCT CGCCGGAGGG CCAGCTGGAG GTGCAGACGG TCATCGTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005255213.1
    CDS117..2627
    Translation

    Target ORF information:

    RefSeq Version XM_005255156.1
    Organism Homo sapiens(human)
    Definition Homo sapiens E4F transcription factor 1 (E4F1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005255156.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC
    GGCTTCCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGGTA ACCGGGCCGA CCCGGAGGGT
    GCGGCCGGGG TGCGGGCAGT TCATCCCGGG CTGGCAGAGG CCCGGATGGC CCGAGCTGCG
    GGCTCGACCG ACCCTCCCAG ACCCAGACAC CTCGCGGATC TCGCGGGACC CTCACAGCCC
    TCCACGAAAC CCCCAGATGA GGACGATGTG CACAGATGCG GCCGCTGCCA GGCAGAGTTC
    ACCGCCTTGG AGGATTTTGT TCAGCACAAG ATTCAGAAGG CCTGCCAGCG GGCCCCTCCG
    GAGGCCCTGC CTGCCACCCC TGCCACCACA GCGTTGCTGG GCCAGGAGGT GGTGCCGGCA
    GCACCAGGCC CAGAGGAGCC CATCACTGTG GCCCACATCG TGGTGGAGGC GGCCTCTCTG
    GCAGCAGACA TCAGCCACGC ATCTGACCTT GTTGGTGGTG GGCACATCAA AGAGGTCATC
    GTGGCTGCTG AGGCGGAGCT GGGAGACGGT GAGATGGCCG AGGCCCCGGG CAGCCCCCGC
    CAGCAGGGGC TGGGGCTCGC AGGGGAGGGT GAGCAGGCCC AGGTGAAGCT ACTGGTGAAC
    AAGGATGGCC GCTATGTGTG TGCGCTGTGC CACAAGACCT TCAAGACGGG CAGCATCCTC
    AAGGCCCACA TGGTCACTCA CAGCAGCCGC AAGGACCACG AGTGCAAGCT CTGTGGGGCC
    TCCTTCCGCA CCAAGGGCTC ACTCATCCGG CACCACCGGC GGCACACGGA TGAGCGCCCC
    TACAAGTGCT CCAAGTGTGG AAAGAGCTTC CGGGAGTCGG GTGCACTGAC CCGGCACCTC
    AAGTCTCTCA CCCCCTGCAC AGAGAAAATC CGCTTCAGTG TGAGCAAGGA CGTGGTTGTC
    AGCAAAGAGG ACGCACGTGC AGGTTCTGGA GCTGGAGCTG CCGGCTTGGG GACAGCCACA
    TCATCGGTGA CAGGCGAGCC TATAGAGACT TCACCCGTGA TTCACCTGGT GACAGATGCC
    AAGGGCACCG TCATCCACGA AGTCCACGTC CAGATGCAGG AGCTGTCCCT GGGCATGAAA
    GCCCTGGCCC CAGAGCCCCC CGTCTCCCAG GAGCTCCCCT GCTCCAGCGA GGGCAGCCGT
    GAGAACCTGC TGCACCAGGC CATGCAGAAC TCCGGCATCG TCCTTGAGCG CGCTGCTGGG
    GAGGAGGGTG CCCTGGAGCC AGCTCCTGCT GCCGGGTCCA GTCCCCAGCC CCTGGCAGTG
    GCAGCCCCGC AGCTGCCGGT ACTGGAAGTG CAGCCGCTGG AGACAGTGGC CAGCGAGGCC
    TCAGCGGTGC CCAGGACCCA CCCATGTCCT CAGTGCAGTG AGACCTTCCC GACAGCAGCC
    ACCCTGGAGG CCCACAAGAG GGGCCACACC GGGCCGAGGC CGTTCGCCTG CGCGCAGTGT
    GGCAAGGCCT TCCCCAAGGC CTACCTGCTC AAGAAGCACC AGGAGGTGCA CGTGCGTGAG
    CGCCGCTTCC GCTGTGGCGA CTGCGGGAAG CTCTACAAGA CCATTGCCCA TGTGCGTGGC
    CACCGGCGCG TCCACTCAGA CGAGCGGCCC TACCCTTGTC CCAAGTGTGG CAAGCGCTAC
    AAGACTAAGA ACGCACAGCA GGTGCACTTC AGGACACACC TGGAGGAGAA GCCGCACGTG
    TGCCAGTTCT GCAGCCGTGG CTTCCGAGAG AAGGGCTCAC TGGTGCGGCA CGTGCGACAC
    CACACAGGCG AGAAGCCGTT CAAGTGCTAC AAGTGCGGCC GTGGCTTCGC CGAGCACGGC
    ACGCTGAACC GGCACCTGCG CACCAAAGGG GGCTGCCTGC TGGAGGTGGA GGAGTTGCTG
    GTGTCTGAGG ACAGCCCCGC GGCAGCCACC ACCGTCCTCA CGGAAGACCC GCACACAGTG
    TTGGTGGAGT TCTCGTCCGT GGTAGCTGAC ACCCAGGAGT ATATCATCGA GGCCACTGCG
    GACGATGCGG AGACCAGTGA GGCCACGGAG ATCATCGAGG GCACCCAGAC AGAGGTGGAC
    AGCCACATCA TGAAGGTGGT GCAGCAGATC GTGCACCAGG CTAGCGCCGG CCACCAGATC
    ATCGTGCAGA ACGTCACCAT GGACGAGGAG ACGGCGCTGG GCCCAGAGGC GGCTGCCGCC
    GACACCATCA CCATCGCCAC CCCCGAGAGC CTGACAGAGC AGGTGGCCAT GACGCTGGCC
    TCGGCCATCA GCGAGGGCAC TGTGCTTGCC GCCCGGGCAG GGACAAGTGG CACTGAACAG
    GCCACTGTGA CCATGGTGTC ATCAGAGGAC ATCGAGATCC TGGAGCATGC AGGCGAGCTG
    GTCATCGCCT CGCCGGAGGG CCAGCTGGAG GTGCAGACGG TCATCGTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu48778
    Clone ID Related Accession (Same CDS sequence) XM_006720858.1
    Accession Version XM_006720858.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2352bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product transcription factor E4F1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC
    GGCTTCCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGATGT GCACAGATGC
    GGCCGCTGCC AGGCAGAGTT CACCGCCTTG GAGGATTTTG TTCAGCACAA GATTCAGAAG
    GCCTGCCAGC GGGCCCCTCC GGAGGCCCTG CCTGCCACCC CTGCCACCAC AGCGTTGCTG
    GGCCAGGAGG TGGTGCCGGC AGCACCAGGC CCAGAGGAGC CCATCACTGT GGCCCACATC
    GTGGTGGAGG CGGCCTCTCT GGCAGCAGAC ATCAGCCACG CATCTGACCT TGTTGGTGGT
    GGGCACATCA AAGAGGTCAT CGTGGCTGCT GAGGCGGAGC TGGGAGACGG TGAGATGGCC
    GAGGCCCCGG GCAGCCCCCG CCAGCAGGGG CTGGGGCTCG CAGGGGAGGG TGAGCAGGCC
    CAGGTGAAGC TACTGGTGAA CAAGGATGGC CGCTATGTGT GTGCGCTGTG CCACAAGACC
    TTCAAGACGG GCAGCATCCT CAAGGCCCAC ATGGTCACTC ACAGCAGCCG CAAGGACCAC
    GAGTGCAAGC TCTGTGGGGC CTCCTTCCGC ACCAAGGGCT CACTCATCCG GCACCACCGG
    CGGCACACGG ATGAGCGCCC CTACAAGTGC TCCAAGTGTG GAAAGAGCTT CCGGGAGTCG
    GGTGCACTGA CCCGGCACCT CAAGTCTCTC ACCCCCTGCA CAGAGAAAAT CCGCTTCAGT
    GTGAGCAAGG ACGTGGTTGT CAGCAAAGAG GACGCACGTG CAGGTTCTGG AGCTGGAGCT
    GCCGGCTTGG GGACAGCCAC ATCATCGGTG ACAGGCGAGC CTATAGAGAC TTCACCCGTG
    ATTCACCTGG TGACAGATGC CAAGGGCACC GTCATCCACG AAGTCCACGT CCAGATGCAG
    GAGCTGTCCC TGGGCATGAA AGCCCTGGCC CCAGAGCCCC CCGTCTCCCA GGAGCTCCCC
    TGCTCCAGCG AGGGCAGCCG TGAGAACCTG CTGCACCAGG CCATGCAGAA CTCCGGCATC
    GTCCTTGAGC GCGCTGCTGG GGAGGAGGGT GCCCTGGAGC CAGCTCCTGC TGCCGGGTCC
    AGTCCCCAGC CCCTGGCAGT GGCAGCCCCG CAGCTGCCGG TACTGGAAGT GCAGCCGCTG
    GAGACAGTGG CCAGCGAGGC CTCAGCGGTG CCCAGGACCC ACCCATGTCC TCAGTGCAGT
    GAGACCTTCC CGACAGCAGC CACCCTGGAG GCCCACAAGA GGGGCCACAC CGGGCCGAGG
    CCGTTCGCCT GCGCGCAGTG TGGCAAGGCC TTCCCCAAGG CCTACCTGCT CAAGAAGCAC
    CAGGAGGTGC ACGTGCGTGA GCGCCGCTTC CGCTGTGGCG ACTGCGGGAA GCTCTACAAG
    ACCATTGCCC ATGTGCGTGG CCACCGGCGC GTCCACTCAG ACGAGCGGCC CTACCCTTGT
    CCCAAGTGTG GCAAGCGCTA CAAGACTAAG AACGCACAGC AGGTGCACTT CAGGACACAC
    CTGGAGGAGA AGCCGCACGT GTGCCAGTTC TGCAGCCGTG GCTTCCGAGA GAAGGGCTCA
    CTGGTGCGGC ACGTGCGACA CCACACAGGC GAGAAGCCGT TCAAGTGCTA CAAGTGCGGC
    CGTGGCTTCG CCGAGCACGG CACGCTGAAC CGGCACCTGC GCACCAAAGG GGGCTGCCTG
    CTGGAGGTGG AGGAGTTGCT GGTGTCTGAG GACAGCCCCG CGGCAGCCAC CACCGTCCTC
    ACGGAAGACC CGCACACAGT GTTGGTGGAG TTCTCGTCCG TGGTAGCTGA CACCCAGGAG
    TATATCATCG AGGCCACTGC GGACGATGCG GAGACCAGTG AGGCCACGGA GATCATCGAG
    GGCACCCAGA CAGAGGTGGA CAGCCACATC ATGAAGGTGG TGCAGCAGAT CGTGCACCAG
    GCTAGCGCCG GCCACCAGAT CATCGTGCAG AACGTCACCA TGGACGAGGA GACGGCGCTG
    GGCCCAGAGG CGGCTGCCGC CGACACCATC ACCATCGCCA CCCCCGAGAG CCTGACAGAG
    CAGGTGGCCA TGACGCTGGC CTCGGCCATC AGCGAGGGCA CTGTGCTTGC CGCCCGGGCA
    GGGACAAGTG GCACTGAACA GGCCACTGTG ACCATGGTGT CATCAGAGGA CATCGAGATC
    CTGGAGCATG CAGGCGAGCT GGTCATCGCC TCGCCGGAGG GCCAGCTGGA GGTGCAGACG
    GTCATCGTCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006720921.1
    CDS49..2400
    Translation

    Target ORF information:

    RefSeq Version XM_006720858.1
    Organism Homo sapiens(human)
    Definition Homo sapiens E4F transcription factor 1 (E4F1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006720858.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC
    GGCTTCCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGATGT GCACAGATGC
    GGCCGCTGCC AGGCAGAGTT CACCGCCTTG GAGGATTTTG TTCAGCACAA GATTCAGAAG
    GCCTGCCAGC GGGCCCCTCC GGAGGCCCTG CCTGCCACCC CTGCCACCAC AGCGTTGCTG
    GGCCAGGAGG TGGTGCCGGC AGCACCAGGC CCAGAGGAGC CCATCACTGT GGCCCACATC
    GTGGTGGAGG CGGCCTCTCT GGCAGCAGAC ATCAGCCACG CATCTGACCT TGTTGGTGGT
    GGGCACATCA AAGAGGTCAT CGTGGCTGCT GAGGCGGAGC TGGGAGACGG TGAGATGGCC
    GAGGCCCCGG GCAGCCCCCG CCAGCAGGGG CTGGGGCTCG CAGGGGAGGG TGAGCAGGCC
    CAGGTGAAGC TACTGGTGAA CAAGGATGGC CGCTATGTGT GTGCGCTGTG CCACAAGACC
    TTCAAGACGG GCAGCATCCT CAAGGCCCAC ATGGTCACTC ACAGCAGCCG CAAGGACCAC
    GAGTGCAAGC TCTGTGGGGC CTCCTTCCGC ACCAAGGGCT CACTCATCCG GCACCACCGG
    CGGCACACGG ATGAGCGCCC CTACAAGTGC TCCAAGTGTG GAAAGAGCTT CCGGGAGTCG
    GGTGCACTGA CCCGGCACCT CAAGTCTCTC ACCCCCTGCA CAGAGAAAAT CCGCTTCAGT
    GTGAGCAAGG ACGTGGTTGT CAGCAAAGAG GACGCACGTG CAGGTTCTGG AGCTGGAGCT
    GCCGGCTTGG GGACAGCCAC ATCATCGGTG ACAGGCGAGC CTATAGAGAC TTCACCCGTG
    ATTCACCTGG TGACAGATGC CAAGGGCACC GTCATCCACG AAGTCCACGT CCAGATGCAG
    GAGCTGTCCC TGGGCATGAA AGCCCTGGCC CCAGAGCCCC CCGTCTCCCA GGAGCTCCCC
    TGCTCCAGCG AGGGCAGCCG TGAGAACCTG CTGCACCAGG CCATGCAGAA CTCCGGCATC
    GTCCTTGAGC GCGCTGCTGG GGAGGAGGGT GCCCTGGAGC CAGCTCCTGC TGCCGGGTCC
    AGTCCCCAGC CCCTGGCAGT GGCAGCCCCG CAGCTGCCGG TACTGGAAGT GCAGCCGCTG
    GAGACAGTGG CCAGCGAGGC CTCAGCGGTG CCCAGGACCC ACCCATGTCC TCAGTGCAGT
    GAGACCTTCC CGACAGCAGC CACCCTGGAG GCCCACAAGA GGGGCCACAC CGGGCCGAGG
    CCGTTCGCCT GCGCGCAGTG TGGCAAGGCC TTCCCCAAGG CCTACCTGCT CAAGAAGCAC
    CAGGAGGTGC ACGTGCGTGA GCGCCGCTTC CGCTGTGGCG ACTGCGGGAA GCTCTACAAG
    ACCATTGCCC ATGTGCGTGG CCACCGGCGC GTCCACTCAG ACGAGCGGCC CTACCCTTGT
    CCCAAGTGTG GCAAGCGCTA CAAGACTAAG AACGCACAGC AGGTGCACTT CAGGACACAC
    CTGGAGGAGA AGCCGCACGT GTGCCAGTTC TGCAGCCGTG GCTTCCGAGA GAAGGGCTCA
    CTGGTGCGGC ACGTGCGACA CCACACAGGC GAGAAGCCGT TCAAGTGCTA CAAGTGCGGC
    CGTGGCTTCG CCGAGCACGG CACGCTGAAC CGGCACCTGC GCACCAAAGG GGGCTGCCTG
    CTGGAGGTGG AGGAGTTGCT GGTGTCTGAG GACAGCCCCG CGGCAGCCAC CACCGTCCTC
    ACGGAAGACC CGCACACAGT GTTGGTGGAG TTCTCGTCCG TGGTAGCTGA CACCCAGGAG
    TATATCATCG AGGCCACTGC GGACGATGCG GAGACCAGTG AGGCCACGGA GATCATCGAG
    GGCACCCAGA CAGAGGTGGA CAGCCACATC ATGAAGGTGG TGCAGCAGAT CGTGCACCAG
    GCTAGCGCCG GCCACCAGAT CATCGTGCAG AACGTCACCA TGGACGAGGA GACGGCGCTG
    GGCCCAGAGG CGGCTGCCGC CGACACCATC ACCATCGCCA CCCCCGAGAG CCTGACAGAG
    CAGGTGGCCA TGACGCTGGC CTCGGCCATC AGCGAGGGCA CTGTGCTTGC CGCCCGGGCA
    GGGACAAGTG GCACTGAACA GGCCACTGTG ACCATGGTGT CATCAGAGGA CATCGAGATC
    CTGGAGCATG CAGGCGAGCT GGTCATCGCC TCGCCGGAGG GCCAGCTGGA GGTGCAGACG
    GTCATCGTCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57414
    Clone ID Related Accession (Same CDS sequence) XM_011522402.1
    Accession Version XM_011522402.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2484bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product transcription factor E4F1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC
    GGCTTCCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGGTA ACCGGGCCGA CCCGGAGGGT
    GCGGCCGGGG TGCGGGCAGT TCATCCCGGG CTGGCAGAGG CCCGGATGGC CCGAGCTGCG
    GGCTCGACCG ACCCTCCCAG ACCCAGACAC CTCGCGGATC TCGCGGGACC CTCACAGCCC
    TCCACGAAAC CCCCAGATGA GGACGATGTG CACAGATGCG GCCGCTGCCA GGCAGAGTTC
    ACCGCCTTGG AGGATTTTGT TCAGCACAAG ATTCAGAAGG CCTGCCAGCG GGCCCCTCCG
    GAGGCCCTGC CTGCCACCCC TGCCACCACA GCGTTGCTGG GCCAGGAGGT GGTGCCGGCA
    GCACCAGGCC CAGAGGAGCC CATCACTGTG GCCCACATCG TGGTGGAGGC GGCCTCTCTG
    GCAGCAGACA TCAGCCACGC ATCTGACCTT GTTGGTGGTG GGCACATCAA AGAGGTCATC
    GTGGCTGCTG AGGCGGAGCT GGGAGACGGT GAGATGGCCG AGGCCCCGGG CAGCCCCCGC
    CAGCAGGGGC TGGGGCTCGC AGGGGAGGGT GAGCAGGCCC AGGTGAAGCT ACTGGTGAAC
    AAGGATGGCC GCTATGTGTG TGCGCTGTGC CACAAGACCT TCAAGACGGG CAGCATCCTC
    AAGGCCCACA TGGTCACTCA CAGCAGCCGC AAGGACCACG AGTGCAAGCT CTGTGGGGCC
    TCCTTCCGCA CCAAGGGCTC ACTCATCCGG CACCACCGGC GGCACACGGA TGAGCGCCCC
    TACAAGTGCT CCAAGTGTGG AAAGAGCTTC CGGGAGTCGG GTGCACTGAC CCGGCACCTC
    AAGTCTCTCA CCCCCTGCAC AGAGAAAATC CGCTTCAGTG TGAGCAAGGA CGTGGTTGTC
    AGCAAAGAGG ACGCACGTGC AGGTTCTGGA GCTGGAGCTG CCGGCTTGGG GACAGCCACA
    TCATCGGTGA CAGGCGAGCC TATAGAGACT TCACCCGTGA TTCACCTGGT GACAGATGCC
    AAGGGCACCG TCATCCACGA AGTCCACGTC CAGATGCAGG AGCTGTCCCT GGGCATGAAA
    GCCCTGGCCC CAGAGCCCCC CGTCTCCCAG GAGCTCCCCT GCTCCAGCGA GGGCAGCCGT
    GAGAACCTGC TGCACCAGGC CATGCAGAAC TCCGGCATCG TCCTTGAGCG CGCTGCTGGG
    GAGGAGGGTG CCCTGGAGCC AGCTCCTGCT GCCGGGTCCA GTCCCCAGCC CCTGGCAGTG
    GCAGCCCCGC AGCTGCCGGT ACTGGAAGTG CAGCCGCTGG AGACACAGGT GGCCAGCGAG
    GCCTCAGCGG TGCCCAGGAC CCACCCATGT CCTCAGTGCA GTGAGACCTT CCCGACAGCA
    GCCACCCTGG AGGCCCACAA GAGGGGCCAC ACCGGGCCGA GGCCGTTCGC CTGCGCGCAG
    TGTGGCAAGG CCTTCCCCAA GGCCTACCTG CTCAAGAAGC ACCAGGAGGT GCACGTGCGT
    GAGCGCCGCT TCCGCTGTGG CGACTGCGGG AAGCTCTACA AGACCATTGC CCATGTGCGT
    GGCCACCGGC GCGTCCACTC AGACGAGCGG CCCTACCCTT GTCCCAAGTG TGGCAAGCGC
    TACAAGACTA AGAACGCACA GCAGGTGCAC TTCAGGACAC ACCTGGAGGA GAAGCCGCAC
    GTGTGCCAGT TCTGCAGCCG TGGCTTCCGA GAGAAGGGCT CACTGGTGCG GCACGTGCGA
    CACCACACAG GCGAGAAGCC GTTCAAGTGC TACAAGTGCG GCCGTGGCTT CGCCGAGCAC
    GGCACGCTGA ACCGGCACCT GCGCACCAAA GGGGGCTGCC TGCTGGAGGT GGAGGAGTTG
    CTGGTGTCTG AGGACAGCCC CGCGGCAGCC ACCACCGTCC TCACGGAAGA CCCGCACACA
    GTGTTGGTGG AGTTCTCGTC CGTGGCCACT GCGGACGATG CGGAGACCAG TGAGGCCACG
    GAGATCATCG AGGGCACCCA GACAGAGGTG GACAGCCACA TCATGAAGGT GGTGCAGCAG
    ATCGTGCACC AGGCTAGCGC CGGCCACCAG ATCATCGTGC AGAACGTCAC CATGGACGAG
    GAGACGGCGC TGGGCCCAGA GGCGGCTGCC GCCGACACCA TCACCATCGC CACCCCCGAG
    AGCCTGACAG AGCAGGTGGC CATGACGCTG GCCTCGGCCA TCAGCGAGGG CACTGTGCTT
    GCCGCCCGGG CAGGGACAAG TGGCACTGAA CAGGCCACTG TGACCATGGT GTCATCAGAG
    GACATCGAGA TCCTGGAGCA TGCAGGCGAG CTGGTCATCG CCTCGCCGGA GGGCCAGCTG
    GAGGTGCAGA CGGTCATCGT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011520704.1
    CDS117..2600
    Translation

    Target ORF information:

    RefSeq Version XM_011522402.1
    Organism Homo sapiens(human)
    Definition Homo sapiens E4F transcription factor 1 (E4F1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011522402.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC
    GGCTTCCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGGTA ACCGGGCCGA CCCGGAGGGT
    GCGGCCGGGG TGCGGGCAGT TCATCCCGGG CTGGCAGAGG CCCGGATGGC CCGAGCTGCG
    GGCTCGACCG ACCCTCCCAG ACCCAGACAC CTCGCGGATC TCGCGGGACC CTCACAGCCC
    TCCACGAAAC CCCCAGATGA GGACGATGTG CACAGATGCG GCCGCTGCCA GGCAGAGTTC
    ACCGCCTTGG AGGATTTTGT TCAGCACAAG ATTCAGAAGG CCTGCCAGCG GGCCCCTCCG
    GAGGCCCTGC CTGCCACCCC TGCCACCACA GCGTTGCTGG GCCAGGAGGT GGTGCCGGCA
    GCACCAGGCC CAGAGGAGCC CATCACTGTG GCCCACATCG TGGTGGAGGC GGCCTCTCTG
    GCAGCAGACA TCAGCCACGC ATCTGACCTT GTTGGTGGTG GGCACATCAA AGAGGTCATC
    GTGGCTGCTG AGGCGGAGCT GGGAGACGGT GAGATGGCCG AGGCCCCGGG CAGCCCCCGC
    CAGCAGGGGC TGGGGCTCGC AGGGGAGGGT GAGCAGGCCC AGGTGAAGCT ACTGGTGAAC
    AAGGATGGCC GCTATGTGTG TGCGCTGTGC CACAAGACCT TCAAGACGGG CAGCATCCTC
    AAGGCCCACA TGGTCACTCA CAGCAGCCGC AAGGACCACG AGTGCAAGCT CTGTGGGGCC
    TCCTTCCGCA CCAAGGGCTC ACTCATCCGG CACCACCGGC GGCACACGGA TGAGCGCCCC
    TACAAGTGCT CCAAGTGTGG AAAGAGCTTC CGGGAGTCGG GTGCACTGAC CCGGCACCTC
    AAGTCTCTCA CCCCCTGCAC AGAGAAAATC CGCTTCAGTG TGAGCAAGGA CGTGGTTGTC
    AGCAAAGAGG ACGCACGTGC AGGTTCTGGA GCTGGAGCTG CCGGCTTGGG GACAGCCACA
    TCATCGGTGA CAGGCGAGCC TATAGAGACT TCACCCGTGA TTCACCTGGT GACAGATGCC
    AAGGGCACCG TCATCCACGA AGTCCACGTC CAGATGCAGG AGCTGTCCCT GGGCATGAAA
    GCCCTGGCCC CAGAGCCCCC CGTCTCCCAG GAGCTCCCCT GCTCCAGCGA GGGCAGCCGT
    GAGAACCTGC TGCACCAGGC CATGCAGAAC TCCGGCATCG TCCTTGAGCG CGCTGCTGGG
    GAGGAGGGTG CCCTGGAGCC AGCTCCTGCT GCCGGGTCCA GTCCCCAGCC CCTGGCAGTG
    GCAGCCCCGC AGCTGCCGGT ACTGGAAGTG CAGCCGCTGG AGACACAGGT GGCCAGCGAG
    GCCTCAGCGG TGCCCAGGAC CCACCCATGT CCTCAGTGCA GTGAGACCTT CCCGACAGCA
    GCCACCCTGG AGGCCCACAA GAGGGGCCAC ACCGGGCCGA GGCCGTTCGC CTGCGCGCAG
    TGTGGCAAGG CCTTCCCCAA GGCCTACCTG CTCAAGAAGC ACCAGGAGGT GCACGTGCGT
    GAGCGCCGCT TCCGCTGTGG CGACTGCGGG AAGCTCTACA AGACCATTGC CCATGTGCGT
    GGCCACCGGC GCGTCCACTC AGACGAGCGG CCCTACCCTT GTCCCAAGTG TGGCAAGCGC
    TACAAGACTA AGAACGCACA GCAGGTGCAC TTCAGGACAC ACCTGGAGGA GAAGCCGCAC
    GTGTGCCAGT TCTGCAGCCG TGGCTTCCGA GAGAAGGGCT CACTGGTGCG GCACGTGCGA
    CACCACACAG GCGAGAAGCC GTTCAAGTGC TACAAGTGCG GCCGTGGCTT CGCCGAGCAC
    GGCACGCTGA ACCGGCACCT GCGCACCAAA GGGGGCTGCC TGCTGGAGGT GGAGGAGTTG
    CTGGTGTCTG AGGACAGCCC CGCGGCAGCC ACCACCGTCC TCACGGAAGA CCCGCACACA
    GTGTTGGTGG AGTTCTCGTC CGTGGCCACT GCGGACGATG CGGAGACCAG TGAGGCCACG
    GAGATCATCG AGGGCACCCA GACAGAGGTG GACAGCCACA TCATGAAGGT GGTGCAGCAG
    ATCGTGCACC AGGCTAGCGC CGGCCACCAG ATCATCGTGC AGAACGTCAC CATGGACGAG
    GAGACGGCGC TGGGCCCAGA GGCGGCTGCC GCCGACACCA TCACCATCGC CACCCCCGAG
    AGCCTGACAG AGCAGGTGGC CATGACGCTG GCCTCGGCCA TCAGCGAGGG CACTGTGCTT
    GCCGCCCGGG CAGGGACAAG TGGCACTGAA CAGGCCACTG TGACCATGGT GTCATCAGAG
    GACATCGAGA TCCTGGAGCA TGCAGGCGAG CTGGTCATCG CCTCGCCGGA GGGCCAGCTG
    GAGGTGCAGA CGGTCATCGT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57415
    Clone ID Related Accession (Same CDS sequence) XM_011522403.1
    Accession Version XM_011522403.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2475bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product transcription factor E4F1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGTCGTAA ATCCGCCATC TTCCTGCGGC GCGTTGCGAC ATGGAGGGCG CGATGGCAGT 
    GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA GCCGGGCGGG AAGCGGGCGA
    GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC GGCTTCCTCG GCCTCCCGGC
    GCCCTTCAGC GAGGAAGGGC TGGAGGAAAA GCCAGACGGA GCCCTGGCTG CCCAGCCTCC
    GCCTTCCCAG GCCCTGCTGA CAGCAGGCTC GTTGCAGATG AGGACGATGT GCACAGATGC
    GGCCGCTGCC AGGCAGAGTT CACCGCCTTG GAGGATTTTG TTCAGCACAA GATTCAGAAG
    GCCTGCCAGC GGGCCCCTCC GGAGGCCCTG CCTGCCACCC CTGCCACCAC AGCGTTGCTG
    GGCCAGGAGG TGGTGCCGGC AGCACCAGGC CCAGAGGAGC CCATCACTGT GGCCCACATC
    GTGGTGGAGG CGGCCTCTCT GGCAGCAGAC ATCAGCCACG CATCTGACCT TGTTGGTGGT
    GGGCACATCA AAGAGGTCAT CGTGGCTGCT GAGGCGGAGC TGGGAGACGG TGAGATGGCC
    GAGGCCCCGG GCAGCCCCCG CCAGCAGGGG CTGGGGCTCG CAGGGGAGGG TGAGCAGGCC
    CAGGTGAAGC TACTGGTGAA CAAGGATGGC CGCTATGTGT GTGCGCTGTG CCACAAGACC
    TTCAAGACGG GCAGCATCCT CAAGGCCCAC ATGGTCACTC ACAGCAGCCG CAAGGACCAC
    GAGTGCAAGC TCTGTGGGGC CTCCTTCCGC ACCAAGGGCT CACTCATCCG GCACCACCGG
    CGGCACACGG ATGAGCGCCC CTACAAGTGC TCCAAGTGTG GAAAGAGCTT CCGGGAGTCG
    GGTGCACTGA CCCGGCACCT CAAGTCTCTC ACCCCCTGCA CAGAGAAAAT CCGCTTCAGT
    GTGAGCAAGG ACGTGGTTGT CAGCAAAGAG GACGCACGTG CAGGTTCTGG AGCTGGAGCT
    GCCGGCTTGG GGACAGCCAC ATCATCGGTG ACAGGCGAGC CTATAGAGAC TTCACCCGTG
    ATTCACCTGG TGACAGATGC CAAGGGCACC GTCATCCACG AAGTCCACGT CCAGATGCAG
    GAGCTGTCCC TGGGCATGAA AGCCCTGGCC CCAGAGCCCC CCGTCTCCCA GGAGCTCCCC
    TGCTCCAGCG AGGGCAGCCG TGAGAACCTG CTGCACCAGG CCATGCAGAA CTCCGGCATC
    GTCCTTGAGC GCGCTGCTGG GGAGGAGGGT GCCCTGGAGC CAGCTCCTGC TGCCGGGTCC
    AGTCCCCAGC CCCTGGCAGT GGCAGCCCCG CAGCTGCCGG TACTGGAAGT GCAGCCGCTG
    GAGACACAGG TGGCCAGCGA GGCCTCAGCG GTGCCCAGGA CCCACCCATG TCCTCAGTGC
    AGTGAGACCT TCCCGACAGC AGCCACCCTG GAGGCCCACA AGAGGGGCCA CACCGGGCCG
    AGGCCGTTCG CCTGCGCGCA GTGTGGCAAG GCCTTCCCCA AGGCCTACCT GCTCAAGAAG
    CACCAGGAGG TGCACGTGCG TGAGCGCCGC TTCCGCTGTG GCGACTGCGG GAAGCTCTAC
    AAGACCATTG CCCATGTGCG TGGCCACCGG CGCGTCCACT CAGACGAGCG GCCCTACCCT
    TGTCCCAAGT GTGGCAAGCG CTACAAGACT AAGAACGCAC AGCAGGTGCA CTTCAGGACA
    CACCTGGAGG AGAAGCCGCA CGTGTGCCAG TTCTGCAGCC GTGGCTTCCG AGAGAAGGGC
    TCACTGGTGC GGCACGTGCG ACACCACACA GGCGAGAAGC CGTTCAAGTG CTACAAGTGC
    GGCCGTGGCT TCGCCGAGCA CGGCACGCTG AACCGGCACC TGCGCACCAA AGGGGGCTGC
    CTGCTGGAGG TGGAGGAGTT GCTGGTGTCT GAGGACAGCC CCGCGGCAGC CACCACCGTC
    CTCACGGAAG ACCCGCACAC AGTGTTGGTG GAGTTCTCGT CCGTGGTAGC TGACACCCAG
    GAGTATATCA TCGAGGCCAC TGCGGACGAT GCGGAGACCA GTGAGGCCAC GGAGATCATC
    GAGGGCACCC AGACAGAGGT GGACAGCCAC ATCATGAAGG TGGTGCAGCA GATCGTGCAC
    CAGGCTAGCG CCGGCCACCA GATCATCGTG CAGAACGTCA CCATGGACGA GGAGACGGCG
    CTGGGCCCAG AGGCGGCTGC CGCCGACACC ATCACCATCG CCACCCCCGA GAGCCTGACA
    GAGCAGGTGG CCATGACGCT GGCCTCGGCC ATCAGCGAGG GCACTGTGCT TGCCGCCCGG
    GCAGGGACAA GTGGCACTGA ACAGGCCACT GTGACCATGG TGTCATCAGA GGACATCGAG
    ATCCTGGAGC ATGCAGGCGA GCTGGTCATC GCCTCGCCGG AGGGCCAGCT GGAGGTGCAG
    ACGGTCATCG TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011520705.1
    CDS77..2551
    Translation

    Target ORF information:

    RefSeq Version XM_011522403.1
    Organism Homo sapiens(human)
    Definition Homo sapiens E4F transcription factor 1 (E4F1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011522403.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGTCGTAA ATCCGCCATC TTCCTGCGGC GCGTTGCGAC ATGGAGGGCG CGATGGCAGT 
    GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA GCCGGGCGGG AAGCGGGCGA
    GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC GGCTTCCTCG GCCTCCCGGC
    GCCCTTCAGC GAGGAAGGGC TGGAGGAAAA GCCAGACGGA GCCCTGGCTG CCCAGCCTCC
    GCCTTCCCAG GCCCTGCTGA CAGCAGGCTC GTTGCAGATG AGGACGATGT GCACAGATGC
    GGCCGCTGCC AGGCAGAGTT CACCGCCTTG GAGGATTTTG TTCAGCACAA GATTCAGAAG
    GCCTGCCAGC GGGCCCCTCC GGAGGCCCTG CCTGCCACCC CTGCCACCAC AGCGTTGCTG
    GGCCAGGAGG TGGTGCCGGC AGCACCAGGC CCAGAGGAGC CCATCACTGT GGCCCACATC
    GTGGTGGAGG CGGCCTCTCT GGCAGCAGAC ATCAGCCACG CATCTGACCT TGTTGGTGGT
    GGGCACATCA AAGAGGTCAT CGTGGCTGCT GAGGCGGAGC TGGGAGACGG TGAGATGGCC
    GAGGCCCCGG GCAGCCCCCG CCAGCAGGGG CTGGGGCTCG CAGGGGAGGG TGAGCAGGCC
    CAGGTGAAGC TACTGGTGAA CAAGGATGGC CGCTATGTGT GTGCGCTGTG CCACAAGACC
    TTCAAGACGG GCAGCATCCT CAAGGCCCAC ATGGTCACTC ACAGCAGCCG CAAGGACCAC
    GAGTGCAAGC TCTGTGGGGC CTCCTTCCGC ACCAAGGGCT CACTCATCCG GCACCACCGG
    CGGCACACGG ATGAGCGCCC CTACAAGTGC TCCAAGTGTG GAAAGAGCTT CCGGGAGTCG
    GGTGCACTGA CCCGGCACCT CAAGTCTCTC ACCCCCTGCA CAGAGAAAAT CCGCTTCAGT
    GTGAGCAAGG ACGTGGTTGT CAGCAAAGAG GACGCACGTG CAGGTTCTGG AGCTGGAGCT
    GCCGGCTTGG GGACAGCCAC ATCATCGGTG ACAGGCGAGC CTATAGAGAC TTCACCCGTG
    ATTCACCTGG TGACAGATGC CAAGGGCACC GTCATCCACG AAGTCCACGT CCAGATGCAG
    GAGCTGTCCC TGGGCATGAA AGCCCTGGCC CCAGAGCCCC CCGTCTCCCA GGAGCTCCCC
    TGCTCCAGCG AGGGCAGCCG TGAGAACCTG CTGCACCAGG CCATGCAGAA CTCCGGCATC
    GTCCTTGAGC GCGCTGCTGG GGAGGAGGGT GCCCTGGAGC CAGCTCCTGC TGCCGGGTCC
    AGTCCCCAGC CCCTGGCAGT GGCAGCCCCG CAGCTGCCGG TACTGGAAGT GCAGCCGCTG
    GAGACACAGG TGGCCAGCGA GGCCTCAGCG GTGCCCAGGA CCCACCCATG TCCTCAGTGC
    AGTGAGACCT TCCCGACAGC AGCCACCCTG GAGGCCCACA AGAGGGGCCA CACCGGGCCG
    AGGCCGTTCG CCTGCGCGCA GTGTGGCAAG GCCTTCCCCA AGGCCTACCT GCTCAAGAAG
    CACCAGGAGG TGCACGTGCG TGAGCGCCGC TTCCGCTGTG GCGACTGCGG GAAGCTCTAC
    AAGACCATTG CCCATGTGCG TGGCCACCGG CGCGTCCACT CAGACGAGCG GCCCTACCCT
    TGTCCCAAGT GTGGCAAGCG CTACAAGACT AAGAACGCAC AGCAGGTGCA CTTCAGGACA
    CACCTGGAGG AGAAGCCGCA CGTGTGCCAG TTCTGCAGCC GTGGCTTCCG AGAGAAGGGC
    TCACTGGTGC GGCACGTGCG ACACCACACA GGCGAGAAGC CGTTCAAGTG CTACAAGTGC
    GGCCGTGGCT TCGCCGAGCA CGGCACGCTG AACCGGCACC TGCGCACCAA AGGGGGCTGC
    CTGCTGGAGG TGGAGGAGTT GCTGGTGTCT GAGGACAGCC CCGCGGCAGC CACCACCGTC
    CTCACGGAAG ACCCGCACAC AGTGTTGGTG GAGTTCTCGT CCGTGGTAGC TGACACCCAG
    GAGTATATCA TCGAGGCCAC TGCGGACGAT GCGGAGACCA GTGAGGCCAC GGAGATCATC
    GAGGGCACCC AGACAGAGGT GGACAGCCAC ATCATGAAGG TGGTGCAGCA GATCGTGCAC
    CAGGCTAGCG CCGGCCACCA GATCATCGTG CAGAACGTCA CCATGGACGA GGAGACGGCG
    CTGGGCCCAG AGGCGGCTGC CGCCGACACC ATCACCATCG CCACCCCCGA GAGCCTGACA
    GAGCAGGTGG CCATGACGCT GGCCTCGGCC ATCAGCGAGG GCACTGTGCT TGCCGCCCGG
    GCAGGGACAA GTGGCACTGA ACAGGCCACT GTGACCATGG TGTCATCAGA GGACATCGAG
    ATCCTGGAGC ATGCAGGCGA GCTGGTCATC GCCTCGCCGG AGGGCCAGCT GGAGGTGCAG
    ACGGTCATCG TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu107368
    Clone ID Related Accession (Same CDS sequence) NM_004424.5
    Accession Version NM_004424.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2355bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-26
    Organism Homo sapiens(human)
    Product transcription factor E4F1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009065.8. On Nov 23, 2018 this sequence version replaced NM_004424.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC080524.1, SRR3476690.890332.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000301727.9/ ENSP00000301727.4 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC
    GGCTTCCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGATGT GCACAGATGC
    GGCCGCTGCC AGGCAGAGTT CACCGCCTTG GAGGATTTTG TTCAGCACAA GATTCAGAAG
    GCCTGCCAGC GGGCCCCTCC GGAGGCCCTG CCTGCCACCC CTGCCACCAC AGCGTTGCTG
    GGCCAGGAGG TGGTGCCGGC AGCACCAGGC CCAGAGGAGC CCATCACTGT GGCCCACATC
    GTGGTGGAGG CGGCCTCTCT GGCAGCAGAC ATCAGCCACG CATCTGACCT TGTTGGTGGT
    GGGCACATCA AAGAGGTCAT CGTGGCTGCT GAGGCGGAGC TGGGAGACGG TGAGATGGCC
    GAGGCCCCGG GCAGCCCCCG CCAGCAGGGG CTGGGGCTCG CAGGGGAGGG TGAGCAGGCC
    CAGGTGAAGC TACTGGTGAA CAAGGATGGC CGCTATGTGT GTGCGCTGTG CCACAAGACC
    TTCAAGACGG GCAGCATCCT CAAGGCCCAC ATGGTCACTC ACAGCAGCCG CAAGGACCAC
    GAGTGCAAGC TCTGTGGGGC CTCCTTCCGC ACCAAGGGCT CACTCATCCG GCACCACCGG
    CGGCACACGG ATGAGCGCCC CTACAAGTGC TCCAAGTGTG GAAAGAGCTT CCGGGAGTCG
    GGTGCACTGA CCCGGCACCT CAAGTCTCTC ACCCCCTGCA CAGAGAAAAT CCGCTTCAGT
    GTGAGCAAGG ACGTGGTTGT CAGCAAAGAG GACGCACGTG CAGGTTCTGG AGCTGGAGCT
    GCCGGCTTGG GGACAGCCAC ATCATCGGTG ACAGGCGAGC CTATAGAGAC TTCACCCGTG
    ATTCACCTGG TGACAGATGC CAAGGGCACC GTCATCCACG AAGTCCACGT CCAGATGCAG
    GAGCTGTCCC TGGGCATGAA AGCCCTGGCC CCAGAGCCCC CCGTCTCCCA GGAGCTCCCC
    TGCTCCAGCG AGGGCAGCCG TGAGAACCTG CTGCACCAGG CCATGCAGAA CTCCGGCATC
    GTCCTTGAGC GCGCTGCTGG GGAGGAGGGT GCCCTGGAGC CAGCTCCTGC TGCCGGGTCC
    AGTCCCCAGC CCCTGGCAGT GGCAGCCCCG CAGCTGCCGG TACTGGAAGT GCAGCCGCTG
    GAGACACAGG TGGCCAGCGA GGCCTCAGCG GTGCCCAGGA CCCACCCATG TCCTCAGTGC
    AGTGAGACCT TCCCGACAGC AGCCACCCTG GAGGCCCACA AGAGGGGCCA CACCGGGCCG
    AGGCCGTTCG CCTGCGCGCA GTGTGGCAAG GCCTTCCCCA AGGCCTACCT GCTCAAGAAG
    CACCAGGAGG TGCACGTGCG TGAGCGCCGC TTCCGCTGTG GCGACTGCGG GAAGCTCTAC
    AAGACCATTG CCCATGTGCG TGGCCACCGG CGCGTCCACT CAGACGAGCG GCCCTACCCT
    TGTCCCAAGT GTGGCAAGCG CTACAAGACT AAGAACGCAC AGCAGGTGCA CTTCAGGACA
    CACCTGGAGG AGAAGCCGCA CGTGTGCCAG TTCTGCAGCC GTGGCTTCCG AGAGAAGGGC
    TCACTGGTGC GGCACGTGCG ACACCACACA GGCGAGAAGC CGTTCAAGTG CTACAAGTGC
    GGCCGTGGCT TCGCCGAGCA CGGCACGCTG AACCGGCACC TGCGCACCAA AGGGGGCTGC
    CTGCTGGAGG TGGAGGAGTT GCTGGTGTCT GAGGACAGCC CCGCGGCAGC CACCACCGTC
    CTCACGGAAG ACCCGCACAC AGTGTTGGTG GAGTTCTCGT CCGTGGTAGC TGACACCCAG
    GAGTATATCA TCGAGGCCAC TGCGGACGAT GCGGAGACCA GTGAGGCCAC GGAGATCATC
    GAGGGCACCC AGACAGAGGT GGACAGCCAC ATCATGAAGG TGGTGCAGCA GATCGTGCAC
    CAGGCTAGCG CCGGCCACCA GATCATCGTG CAGAACGTCA CCATGGACGA GGAGACGGCG
    CTGGGCCCAG AGGCGGCTGC CGCCGACACC ATCACCATCG CCACCCCCGA GAGCCTGACA
    GAGCAGGTGG CCATGACGCT GGCCTCGGCC ATCAGCGAGG GCACTGTGCT TGCCGCCCGG
    GCAGGGACAA GTGGCACTGA ACAGGCCACT GTGACCATGG TGTCATCAGA GGACATCGAG
    ATCCTGGAGC ATGCAGGCGA GCTGGTCATC GCCTCGCCGG AGGGCCAGCT GGAGGTGCAG
    ACGGTCATCG TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_004415.4
    CDS24..2378
    Translation

    Target ORF information:

    RefSeq Version NM_004424.5
    Organism Homo sapiens(human)
    Definition Homo sapiens E4F transcription factor 1 (E4F1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_004424.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC
    GGCTTCCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGATGT GCACAGATGC
    GGCCGCTGCC AGGCAGAGTT CACCGCCTTG GAGGATTTTG TTCAGCACAA GATTCAGAAG
    GCCTGCCAGC GGGCCCCTCC GGAGGCCCTG CCTGCCACCC CTGCCACCAC AGCGTTGCTG
    GGCCAGGAGG TGGTGCCGGC AGCACCAGGC CCAGAGGAGC CCATCACTGT GGCCCACATC
    GTGGTGGAGG CGGCCTCTCT GGCAGCAGAC ATCAGCCACG CATCTGACCT TGTTGGTGGT
    GGGCACATCA AAGAGGTCAT CGTGGCTGCT GAGGCGGAGC TGGGAGACGG TGAGATGGCC
    GAGGCCCCGG GCAGCCCCCG CCAGCAGGGG CTGGGGCTCG CAGGGGAGGG TGAGCAGGCC
    CAGGTGAAGC TACTGGTGAA CAAGGATGGC CGCTATGTGT GTGCGCTGTG CCACAAGACC
    TTCAAGACGG GCAGCATCCT CAAGGCCCAC ATGGTCACTC ACAGCAGCCG CAAGGACCAC
    GAGTGCAAGC TCTGTGGGGC CTCCTTCCGC ACCAAGGGCT CACTCATCCG GCACCACCGG
    CGGCACACGG ATGAGCGCCC CTACAAGTGC TCCAAGTGTG GAAAGAGCTT CCGGGAGTCG
    GGTGCACTGA CCCGGCACCT CAAGTCTCTC ACCCCCTGCA CAGAGAAAAT CCGCTTCAGT
    GTGAGCAAGG ACGTGGTTGT CAGCAAAGAG GACGCACGTG CAGGTTCTGG AGCTGGAGCT
    GCCGGCTTGG GGACAGCCAC ATCATCGGTG ACAGGCGAGC CTATAGAGAC TTCACCCGTG
    ATTCACCTGG TGACAGATGC CAAGGGCACC GTCATCCACG AAGTCCACGT CCAGATGCAG
    GAGCTGTCCC TGGGCATGAA AGCCCTGGCC CCAGAGCCCC CCGTCTCCCA GGAGCTCCCC
    TGCTCCAGCG AGGGCAGCCG TGAGAACCTG CTGCACCAGG CCATGCAGAA CTCCGGCATC
    GTCCTTGAGC GCGCTGCTGG GGAGGAGGGT GCCCTGGAGC CAGCTCCTGC TGCCGGGTCC
    AGTCCCCAGC CCCTGGCAGT GGCAGCCCCG CAGCTGCCGG TACTGGAAGT GCAGCCGCTG
    GAGACACAGG TGGCCAGCGA GGCCTCAGCG GTGCCCAGGA CCCACCCATG TCCTCAGTGC
    AGTGAGACCT TCCCGACAGC AGCCACCCTG GAGGCCCACA AGAGGGGCCA CACCGGGCCG
    AGGCCGTTCG CCTGCGCGCA GTGTGGCAAG GCCTTCCCCA AGGCCTACCT GCTCAAGAAG
    CACCAGGAGG TGCACGTGCG TGAGCGCCGC TTCCGCTGTG GCGACTGCGG GAAGCTCTAC
    AAGACCATTG CCCATGTGCG TGGCCACCGG CGCGTCCACT CAGACGAGCG GCCCTACCCT
    TGTCCCAAGT GTGGCAAGCG CTACAAGACT AAGAACGCAC AGCAGGTGCA CTTCAGGACA
    CACCTGGAGG AGAAGCCGCA CGTGTGCCAG TTCTGCAGCC GTGGCTTCCG AGAGAAGGGC
    TCACTGGTGC GGCACGTGCG ACACCACACA GGCGAGAAGC CGTTCAAGTG CTACAAGTGC
    GGCCGTGGCT TCGCCGAGCA CGGCACGCTG AACCGGCACC TGCGCACCAA AGGGGGCTGC
    CTGCTGGAGG TGGAGGAGTT GCTGGTGTCT GAGGACAGCC CCGCGGCAGC CACCACCGTC
    CTCACGGAAG ACCCGCACAC AGTGTTGGTG GAGTTCTCGT CCGTGGTAGC TGACACCCAG
    GAGTATATCA TCGAGGCCAC TGCGGACGAT GCGGAGACCA GTGAGGCCAC GGAGATCATC
    GAGGGCACCC AGACAGAGGT GGACAGCCAC ATCATGAAGG TGGTGCAGCA GATCGTGCAC
    CAGGCTAGCG CCGGCCACCA GATCATCGTG CAGAACGTCA CCATGGACGA GGAGACGGCG
    CTGGGCCCAG AGGCGGCTGC CGCCGACACC ATCACCATCG CCACCCCCGA GAGCCTGACA
    GAGCAGGTGG CCATGACGCT GGCCTCGGCC ATCAGCGAGG GCACTGTGCT TGCCGCCCGG
    GCAGGGACAA GTGGCACTGA ACAGGCCACT GTGACCATGG TGTCATCAGA GGACATCGAG
    ATCCTGGAGC ATGCAGGCGA GCTGGTCATC GCCTCGCCGG AGGGCCAGCT GGAGGTGCAG
    ACGGTCATCG TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85890
    Clone ID Related Accession (Same CDS sequence) XM_017023010.1
    Accession Version XM_017023010.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2481bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product transcription factor E4F1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC
    GGCTTCCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGGTA ACCGGGCCGA CCCGGAGGGT
    GCGGCCGGGG TGCGGGCAGT TCATCCCGGG CTGGCAGAGG CCCGGATGGC CCGAGCTGCG
    GGCTCGACCG ACCCTCCCAG ACCCAGACAC CTCGCGGATC TCGCGGGACC CTCACAGCCC
    TCCACGAAAC CCCCAGATGA GGACGATGTG CACAGATGCG GCCGCTGCCA GGCAGAGTTC
    ACCGCCTTGG AGGATTTTGT TCAGCACAAG ATTCAGAAGG CCTGCCAGCG GGCCCCTCCG
    GAGGCCCTGC CTGCCACCCC TGCCACCACA GCGTTGCTGG GCCAGGAGGT GGTGCCGGCA
    GCACCAGGCC CAGAGGAGCC CATCACTGTG GCCCACATCG TGGTGGAGGC GGCCTCTCTG
    GCAGCAGACA TCAGCCACGC ATCTGACCTT GTTGGTGGTG GGCACATCAA AGAGGTCATC
    GTGGCTGCTG AGGCGGAGCT GGGAGACGGT GAGATGGCCG AGGCCCCGGG CAGCCCCCGC
    CAGCAGGGGC TGGGGCTCGC AGGGGAGGGT GAGCAGGCCC AGGTGAAGCT ACTGGTGAAC
    AAGGATGGCC GCTATGTGTG TGCGCTGTGC CACAAGACCT TCAAGACGGG CAGCATCCTC
    AAGGCCCACA TGGTCACTCA CAGCAGCCGC AAGGACCACG AGTGCAAGCT CTGTGGGGCC
    TCCTTCCGCA CCAAGGGCTC ACTCATCCGG CACCACCGGC GGCACACGGA TGAGCGCCCC
    TACAAGTGCT CCAAGTGTGG AAAGAGCTTC CGGGAGTCGG GTGCACTGAC CCGGCACCTC
    AAGTCTCTCA CCCCCTGCAC AGAGAAAATC CGCTTCAGTG TGAGCAAGGA CGTGGTTGTC
    AGCAAAGAGG ACGCACGTGC AGGTTCTGGA GCTGGAGCTG CCGGCTTGGG GACAGCCACA
    TCATCGGTGA CAGGCGAGCC TATAGAGACT TCACCCGTGA TTCACCTGGT GACAGATGCC
    AAGGGCACCG TCATCCACGA AGTCCACGTC CAGATGCAGG AGCTGTCCCT GGGCATGAAA
    GCCCTGGCCC CAGAGCCCCC CGTCTCCCAG GAGCTCCCCT GCTCCAGCGA GGGCAGCCGT
    GAGAACCTGC TGCACCAGGC CATGCAGAAC TCCGGCATCG TCCTTGAGCG CGCTGCTGGG
    GAGGAGGGTG CCCTGGAGCC AGCTCCTGCT GCCGGGTCCA GTCCCCAGCC CCTGGCAGTG
    GCAGCCCCGC AGCTGCCGGT ACTGGAAGTG CAGCCGCTGG AGACAGTGGC CAGCGAGGCC
    TCAGCGGTGC CCAGGACCCA CCCATGTCCT CAGTGCAGTG AGACCTTCCC GACAGCAGCC
    ACCCTGGAGG CCCACAAGAG GGGCCACACC GGGCCGAGGC CGTTCGCCTG CGCGCAGTGT
    GGCAAGGCCT TCCCCAAGGC CTACCTGCTC AAGAAGCACC AGGAGGTGCA CGTGCGTGAG
    CGCCGCTTCC GCTGTGGCGA CTGCGGGAAG CTCTACAAGA CCATTGCCCA TGTGCGTGGC
    CACCGGCGCG TCCACTCAGA CGAGCGGCCC TACCCTTGTC CCAAGTGTGG CAAGCGCTAC
    AAGACTAAGA ACGCACAGCA GGTGCACTTC AGGACACACC TGGAGGAGAA GCCGCACGTG
    TGCCAGTTCT GCAGCCGTGG CTTCCGAGAG AAGGGCTCAC TGGTGCGGCA CGTGCGACAC
    CACACAGGCG AGAAGCCGTT CAAGTGCTAC AAGTGCGGCC GTGGCTTCGC CGAGCACGGC
    ACGCTGAACC GGCACCTGCG CACCAAAGGG GGCTGCCTGC TGGAGGTGGA GGAGTTGCTG
    GTGTCTGAGG ACAGCCCCGC GGCAGCCACC ACCGTCCTCA CGGAAGACCC GCACACAGTG
    TTGGTGGAGT TCTCGTCCGT GGCCACTGCG GACGATGCGG AGACCAGTGA GGCCACGGAG
    ATCATCGAGG GCACCCAGAC AGAGGTGGAC AGCCACATCA TGAAGGTGGT GCAGCAGATC
    GTGCACCAGG CTAGCGCCGG CCACCAGATC ATCGTGCAGA ACGTCACCAT GGACGAGGAG
    ACGGCGCTGG GCCCAGAGGC GGCTGCCGCC GACACCATCA CCATCGCCAC CCCCGAGAGC
    CTGACAGAGC AGGTGGCCAT GACGCTGGCC TCGGCCATCA GCGAGGGCAC TGTGCTTGCC
    GCCCGGGCAG GGACAAGTGG CACTGAACAG GCCACTGTGA CCATGGTGTC ATCAGAGGAC
    ATCGAGATCC TGGAGCATGC AGGCGAGCTG GTCATCGCCT CGCCGGAGGG CCAGCTGGAG
    GTGCAGACGG TCATCGTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016878499.1
    CDS117..2597
    Translation

    Target ORF information:

    RefSeq Version XM_017023010.1
    Organism Homo sapiens(human)
    Definition Homo sapiens E4F transcription factor 1 (E4F1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017023010.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC
    GGCTTCCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGGTA ACCGGGCCGA CCCGGAGGGT
    GCGGCCGGGG TGCGGGCAGT TCATCCCGGG CTGGCAGAGG CCCGGATGGC CCGAGCTGCG
    GGCTCGACCG ACCCTCCCAG ACCCAGACAC CTCGCGGATC TCGCGGGACC CTCACAGCCC
    TCCACGAAAC CCCCAGATGA GGACGATGTG CACAGATGCG GCCGCTGCCA GGCAGAGTTC
    ACCGCCTTGG AGGATTTTGT TCAGCACAAG ATTCAGAAGG CCTGCCAGCG GGCCCCTCCG
    GAGGCCCTGC CTGCCACCCC TGCCACCACA GCGTTGCTGG GCCAGGAGGT GGTGCCGGCA
    GCACCAGGCC CAGAGGAGCC CATCACTGTG GCCCACATCG TGGTGGAGGC GGCCTCTCTG
    GCAGCAGACA TCAGCCACGC ATCTGACCTT GTTGGTGGTG GGCACATCAA AGAGGTCATC
    GTGGCTGCTG AGGCGGAGCT GGGAGACGGT GAGATGGCCG AGGCCCCGGG CAGCCCCCGC
    CAGCAGGGGC TGGGGCTCGC AGGGGAGGGT GAGCAGGCCC AGGTGAAGCT ACTGGTGAAC
    AAGGATGGCC GCTATGTGTG TGCGCTGTGC CACAAGACCT TCAAGACGGG CAGCATCCTC
    AAGGCCCACA TGGTCACTCA CAGCAGCCGC AAGGACCACG AGTGCAAGCT CTGTGGGGCC
    TCCTTCCGCA CCAAGGGCTC ACTCATCCGG CACCACCGGC GGCACACGGA TGAGCGCCCC
    TACAAGTGCT CCAAGTGTGG AAAGAGCTTC CGGGAGTCGG GTGCACTGAC CCGGCACCTC
    AAGTCTCTCA CCCCCTGCAC AGAGAAAATC CGCTTCAGTG TGAGCAAGGA CGTGGTTGTC
    AGCAAAGAGG ACGCACGTGC AGGTTCTGGA GCTGGAGCTG CCGGCTTGGG GACAGCCACA
    TCATCGGTGA CAGGCGAGCC TATAGAGACT TCACCCGTGA TTCACCTGGT GACAGATGCC
    AAGGGCACCG TCATCCACGA AGTCCACGTC CAGATGCAGG AGCTGTCCCT GGGCATGAAA
    GCCCTGGCCC CAGAGCCCCC CGTCTCCCAG GAGCTCCCCT GCTCCAGCGA GGGCAGCCGT
    GAGAACCTGC TGCACCAGGC CATGCAGAAC TCCGGCATCG TCCTTGAGCG CGCTGCTGGG
    GAGGAGGGTG CCCTGGAGCC AGCTCCTGCT GCCGGGTCCA GTCCCCAGCC CCTGGCAGTG
    GCAGCCCCGC AGCTGCCGGT ACTGGAAGTG CAGCCGCTGG AGACAGTGGC CAGCGAGGCC
    TCAGCGGTGC CCAGGACCCA CCCATGTCCT CAGTGCAGTG AGACCTTCCC GACAGCAGCC
    ACCCTGGAGG CCCACAAGAG GGGCCACACC GGGCCGAGGC CGTTCGCCTG CGCGCAGTGT
    GGCAAGGCCT TCCCCAAGGC CTACCTGCTC AAGAAGCACC AGGAGGTGCA CGTGCGTGAG
    CGCCGCTTCC GCTGTGGCGA CTGCGGGAAG CTCTACAAGA CCATTGCCCA TGTGCGTGGC
    CACCGGCGCG TCCACTCAGA CGAGCGGCCC TACCCTTGTC CCAAGTGTGG CAAGCGCTAC
    AAGACTAAGA ACGCACAGCA GGTGCACTTC AGGACACACC TGGAGGAGAA GCCGCACGTG
    TGCCAGTTCT GCAGCCGTGG CTTCCGAGAG AAGGGCTCAC TGGTGCGGCA CGTGCGACAC
    CACACAGGCG AGAAGCCGTT CAAGTGCTAC AAGTGCGGCC GTGGCTTCGC CGAGCACGGC
    ACGCTGAACC GGCACCTGCG CACCAAAGGG GGCTGCCTGC TGGAGGTGGA GGAGTTGCTG
    GTGTCTGAGG ACAGCCCCGC GGCAGCCACC ACCGTCCTCA CGGAAGACCC GCACACAGTG
    TTGGTGGAGT TCTCGTCCGT GGCCACTGCG GACGATGCGG AGACCAGTGA GGCCACGGAG
    ATCATCGAGG GCACCCAGAC AGAGGTGGAC AGCCACATCA TGAAGGTGGT GCAGCAGATC
    GTGCACCAGG CTAGCGCCGG CCACCAGATC ATCGTGCAGA ACGTCACCAT GGACGAGGAG
    ACGGCGCTGG GCCCAGAGGC GGCTGCCGCC GACACCATCA CCATCGCCAC CCCCGAGAGC
    CTGACAGAGC AGGTGGCCAT GACGCTGGCC TCGGCCATCA GCGAGGGCAC TGTGCTTGCC
    GCCCGGGCAG GGACAAGTGG CACTGAACAG GCCACTGTGA CCATGGTGTC ATCAGAGGAC
    ATCGAGATCC TGGAGCATGC AGGCGAGCTG GTCATCGCCT CGCCGGAGGG CCAGCTGGAG
    GTGCAGACGG TCATCGTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85891
    Clone ID Related Accession (Same CDS sequence) XM_017023011.1
    Accession Version XM_017023011.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2472bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product transcription factor E4F1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGTCGTAA ATCCGCCATC TTCCTGCGGC GCGTTGCGAC ATGGAGGGCG CGATGGCAGT 
    GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA GCCGGGCGGG AAGCGGGCGA
    GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC GGCTTCCTCG GCCTCCCGGC
    GCCCTTCAGC GAGGAAGGGC TGGAGGAAAA GCCAGACGGA GCCCTGGCTG CCCAGCCTCC
    GCCTTCCCAG GCCCTGCTGA CAGCAGGCTC GTTGCAGATG AGGACGATGT GCACAGATGC
    GGCCGCTGCC AGGCAGAGTT CACCGCCTTG GAGGATTTTG TTCAGCACAA GATTCAGAAG
    GCCTGCCAGC GGGCCCCTCC GGAGGCCCTG CCTGCCACCC CTGCCACCAC AGCGTTGCTG
    GGCCAGGAGG TGGTGCCGGC AGCACCAGGC CCAGAGGAGC CCATCACTGT GGCCCACATC
    GTGGTGGAGG CGGCCTCTCT GGCAGCAGAC ATCAGCCACG CATCTGACCT TGTTGGTGGT
    GGGCACATCA AAGAGGTCAT CGTGGCTGCT GAGGCGGAGC TGGGAGACGG TGAGATGGCC
    GAGGCCCCGG GCAGCCCCCG CCAGCAGGGG CTGGGGCTCG CAGGGGAGGG TGAGCAGGCC
    CAGGTGAAGC TACTGGTGAA CAAGGATGGC CGCTATGTGT GTGCGCTGTG CCACAAGACC
    TTCAAGACGG GCAGCATCCT CAAGGCCCAC ATGGTCACTC ACAGCAGCCG CAAGGACCAC
    GAGTGCAAGC TCTGTGGGGC CTCCTTCCGC ACCAAGGGCT CACTCATCCG GCACCACCGG
    CGGCACACGG ATGAGCGCCC CTACAAGTGC TCCAAGTGTG GAAAGAGCTT CCGGGAGTCG
    GGTGCACTGA CCCGGCACCT CAAGTCTCTC ACCCCCTGCA CAGAGAAAAT CCGCTTCAGT
    GTGAGCAAGG ACGTGGTTGT CAGCAAAGAG GACGCACGTG CAGGTTCTGG AGCTGGAGCT
    GCCGGCTTGG GGACAGCCAC ATCATCGGTG ACAGGCGAGC CTATAGAGAC TTCACCCGTG
    ATTCACCTGG TGACAGATGC CAAGGGCACC GTCATCCACG AAGTCCACGT CCAGATGCAG
    GAGCTGTCCC TGGGCATGAA AGCCCTGGCC CCAGAGCCCC CCGTCTCCCA GGAGCTCCCC
    TGCTCCAGCG AGGGCAGCCG TGAGAACCTG CTGCACCAGG CCATGCAGAA CTCCGGCATC
    GTCCTTGAGC GCGCTGCTGG GGAGGAGGGT GCCCTGGAGC CAGCTCCTGC TGCCGGGTCC
    AGTCCCCAGC CCCTGGCAGT GGCAGCCCCG CAGCTGCCGG TACTGGAAGT GCAGCCGCTG
    GAGACAGTGG CCAGCGAGGC CTCAGCGGTG CCCAGGACCC ACCCATGTCC TCAGTGCAGT
    GAGACCTTCC CGACAGCAGC CACCCTGGAG GCCCACAAGA GGGGCCACAC CGGGCCGAGG
    CCGTTCGCCT GCGCGCAGTG TGGCAAGGCC TTCCCCAAGG CCTACCTGCT CAAGAAGCAC
    CAGGAGGTGC ACGTGCGTGA GCGCCGCTTC CGCTGTGGCG ACTGCGGGAA GCTCTACAAG
    ACCATTGCCC ATGTGCGTGG CCACCGGCGC GTCCACTCAG ACGAGCGGCC CTACCCTTGT
    CCCAAGTGTG GCAAGCGCTA CAAGACTAAG AACGCACAGC AGGTGCACTT CAGGACACAC
    CTGGAGGAGA AGCCGCACGT GTGCCAGTTC TGCAGCCGTG GCTTCCGAGA GAAGGGCTCA
    CTGGTGCGGC ACGTGCGACA CCACACAGGC GAGAAGCCGT TCAAGTGCTA CAAGTGCGGC
    CGTGGCTTCG CCGAGCACGG CACGCTGAAC CGGCACCTGC GCACCAAAGG GGGCTGCCTG
    CTGGAGGTGG AGGAGTTGCT GGTGTCTGAG GACAGCCCCG CGGCAGCCAC CACCGTCCTC
    ACGGAAGACC CGCACACAGT GTTGGTGGAG TTCTCGTCCG TGGTAGCTGA CACCCAGGAG
    TATATCATCG AGGCCACTGC GGACGATGCG GAGACCAGTG AGGCCACGGA GATCATCGAG
    GGCACCCAGA CAGAGGTGGA CAGCCACATC ATGAAGGTGG TGCAGCAGAT CGTGCACCAG
    GCTAGCGCCG GCCACCAGAT CATCGTGCAG AACGTCACCA TGGACGAGGA GACGGCGCTG
    GGCCCAGAGG CGGCTGCCGC CGACACCATC ACCATCGCCA CCCCCGAGAG CCTGACAGAG
    CAGGTGGCCA TGACGCTGGC CTCGGCCATC AGCGAGGGCA CTGTGCTTGC CGCCCGGGCA
    GGGACAAGTG GCACTGAACA GGCCACTGTG ACCATGGTGT CATCAGAGGA CATCGAGATC
    CTGGAGCATG CAGGCGAGCT GGTCATCGCC TCGCCGGAGG GCCAGCTGGA GGTGCAGACG
    GTCATCGTCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016878500.1
    CDS77..2548
    Translation

    Target ORF information:

    RefSeq Version XM_017023011.1
    Organism Homo sapiens(human)
    Definition Homo sapiens E4F transcription factor 1 (E4F1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017023011.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGTCGTAA ATCCGCCATC TTCCTGCGGC GCGTTGCGAC ATGGAGGGCG CGATGGCAGT 
    GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCAGGCCGAA GCCGGGCGGG AAGCGGGCGA
    GGGTGCAGTT GCGGCGGTGG CGGCGGCCTT GGCCCCCAGC GGCTTCCTCG GCCTCCCGGC
    GCCCTTCAGC GAGGAAGGGC TGGAGGAAAA GCCAGACGGA GCCCTGGCTG CCCAGCCTCC
    GCCTTCCCAG GCCCTGCTGA CAGCAGGCTC GTTGCAGATG AGGACGATGT GCACAGATGC
    GGCCGCTGCC AGGCAGAGTT CACCGCCTTG GAGGATTTTG TTCAGCACAA GATTCAGAAG
    GCCTGCCAGC GGGCCCCTCC GGAGGCCCTG CCTGCCACCC CTGCCACCAC AGCGTTGCTG
    GGCCAGGAGG TGGTGCCGGC AGCACCAGGC CCAGAGGAGC CCATCACTGT GGCCCACATC
    GTGGTGGAGG CGGCCTCTCT GGCAGCAGAC ATCAGCCACG CATCTGACCT TGTTGGTGGT
    GGGCACATCA AAGAGGTCAT CGTGGCTGCT GAGGCGGAGC TGGGAGACGG TGAGATGGCC
    GAGGCCCCGG GCAGCCCCCG CCAGCAGGGG CTGGGGCTCG CAGGGGAGGG TGAGCAGGCC
    CAGGTGAAGC TACTGGTGAA CAAGGATGGC CGCTATGTGT GTGCGCTGTG CCACAAGACC
    TTCAAGACGG GCAGCATCCT CAAGGCCCAC ATGGTCACTC ACAGCAGCCG CAAGGACCAC
    GAGTGCAAGC TCTGTGGGGC CTCCTTCCGC ACCAAGGGCT CACTCATCCG GCACCACCGG
    CGGCACACGG ATGAGCGCCC CTACAAGTGC TCCAAGTGTG GAAAGAGCTT CCGGGAGTCG
    GGTGCACTGA CCCGGCACCT CAAGTCTCTC ACCCCCTGCA CAGAGAAAAT CCGCTTCAGT
    GTGAGCAAGG ACGTGGTTGT CAGCAAAGAG GACGCACGTG CAGGTTCTGG AGCTGGAGCT
    GCCGGCTTGG GGACAGCCAC ATCATCGGTG ACAGGCGAGC CTATAGAGAC TTCACCCGTG
    ATTCACCTGG TGACAGATGC CAAGGGCACC GTCATCCACG AAGTCCACGT CCAGATGCAG
    GAGCTGTCCC TGGGCATGAA AGCCCTGGCC CCAGAGCCCC CCGTCTCCCA GGAGCTCCCC
    TGCTCCAGCG AGGGCAGCCG TGAGAACCTG CTGCACCAGG CCATGCAGAA CTCCGGCATC
    GTCCTTGAGC GCGCTGCTGG GGAGGAGGGT GCCCTGGAGC CAGCTCCTGC TGCCGGGTCC
    AGTCCCCAGC CCCTGGCAGT GGCAGCCCCG CAGCTGCCGG TACTGGAAGT GCAGCCGCTG
    GAGACAGTGG CCAGCGAGGC CTCAGCGGTG CCCAGGACCC ACCCATGTCC TCAGTGCAGT
    GAGACCTTCC CGACAGCAGC CACCCTGGAG GCCCACAAGA GGGGCCACAC CGGGCCGAGG
    CCGTTCGCCT GCGCGCAGTG TGGCAAGGCC TTCCCCAAGG CCTACCTGCT CAAGAAGCAC
    CAGGAGGTGC ACGTGCGTGA GCGCCGCTTC CGCTGTGGCG ACTGCGGGAA GCTCTACAAG
    ACCATTGCCC ATGTGCGTGG CCACCGGCGC GTCCACTCAG ACGAGCGGCC CTACCCTTGT
    CCCAAGTGTG GCAAGCGCTA CAAGACTAAG AACGCACAGC AGGTGCACTT CAGGACACAC
    CTGGAGGAGA AGCCGCACGT GTGCCAGTTC TGCAGCCGTG GCTTCCGAGA GAAGGGCTCA
    CTGGTGCGGC ACGTGCGACA CCACACAGGC GAGAAGCCGT TCAAGTGCTA CAAGTGCGGC
    CGTGGCTTCG CCGAGCACGG CACGCTGAAC CGGCACCTGC GCACCAAAGG GGGCTGCCTG
    CTGGAGGTGG AGGAGTTGCT GGTGTCTGAG GACAGCCCCG CGGCAGCCAC CACCGTCCTC
    ACGGAAGACC CGCACACAGT GTTGGTGGAG TTCTCGTCCG TGGTAGCTGA CACCCAGGAG
    TATATCATCG AGGCCACTGC GGACGATGCG GAGACCAGTG AGGCCACGGA GATCATCGAG
    GGCACCCAGA CAGAGGTGGA CAGCCACATC ATGAAGGTGG TGCAGCAGAT CGTGCACCAG
    GCTAGCGCCG GCCACCAGAT CATCGTGCAG AACGTCACCA TGGACGAGGA GACGGCGCTG
    GGCCCAGAGG CGGCTGCCGC CGACACCATC ACCATCGCCA CCCCCGAGAG CCTGACAGAG
    CAGGTGGCCA TGACGCTGGC CTCGGCCATC AGCGAGGGCA CTGTGCTTGC CGCCCGGGCA
    GGGACAAGTG GCACTGAACA GGCCACTGTG ACCATGGTGT CATCAGAGGA CATCGAGATC
    CTGGAGCATG CAGGCGAGCT GGTCATCGCC TCGCCGGAGG GCCAGCTGGA GGTGCAGACG
    GTCATCGTCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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