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C9ORF72 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol C9ORF72
Entrez Gene ID 203228
Full Name chromosome 9 open reading frame 72
Synonyms ALSFTD,DENNL72,FTDALS,FTDALS1
General protein information
Preferred Names

chromosome 9 open reading frame 72

Names

guanine nucleotide exchange C9orf72
protein C9orf72

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

9

9p21.2

Summary The protein encoded by this gene plays an important role in the regulation of endosomal trafficking, and has been shown to interact with Rab proteins that are involved in autophagy and endocytic transport. Expansion of a GGGGCC repeat from 2-22 copies to 700-1600 copies in the intronic sequence between alternate 5' exons in transcripts from this gene is associated with 9p-linked ALS (amyotrophic lateral sclerosis) and FTD (frontotemporal dementia) (PMID: 21944778, 21944779). Studies suggest that hexanucleotide expansions could result in the selective stabilization of repeat-containing pre-mRNA, and the accumulation of insoluble dipeptide repeat protein aggregates that could be pathogenic in FTD-ALS patients (PMID: 23393093). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2016].
Disorder MIM:

614260


mRNA and Protein(s)

mRNA Protein Name
NM_145005.6 NP_659442.2 protein C9orf72 isoform b
NM_018325.4 NP_060795.1 protein C9orf72 isoform a
NM_001256054.2 NP_001242983.1 protein C9orf72 isoform a



Gallus gallus (chicken) C9ORF72 XP_424945.2
Homo sapiens (human) C9orf72 NP_001242983.1
Macaca mulatta (rhesus monkey) C15H9orf72 XP_002800118.1
Pan troglodytes (chimpanzee) C9H9orf72 XP_003951454.1
Canis lupus familiaris (dog) C11H9orf72 XP_005626755.1
Bos taurus (cattle) C8H9orf72 NP_001096558.1
Rattus norvegicus (Norway rat) RGD1359108 NP_001007703.1
Mus musculus (house mouse) 3110043O21Rik NP_001074812.1
Danio rerio (zebrafish) zgc:100846 NP_991166.1


Related articles in PubMed

Bidirectional nucleolar dysfunction in C9orf72 frontotemporal lobar degeneration.
Mizielinska S, Ridler CE, Balendra R, Thoeng A, Woodling NS, Grässer FA, Plagnol V, Lashley T, Partridge L, Isaacs AM
Acta neuropathologica communications5(1)29(2017 Apr)

C9orf72 and RAB7L1 regulate vesicle trafficking in amyotrophic lateral sclerosis and frontotemporal dementia.
Aoki Y, Manzano R, Lee Y, Dafinca R, Aoki M, Douglas AGL, Varela MA, Sathyaprakash C, Scaber J, Barbagallo P, Vader P, Mäger I, Ezzat K, Turner MR, Ito N, Gasco S, Ohbayashi N, El Andaloussi S, Takeda S, Fukuda M, Talbot K, Wood MJA
Brain : a journal of neurology140(4)887-897(2017 Apr)

Multiplex image-based autophagy RNAi screening identifies SMCR8 as ULK1 kinase activity and gene expression regulator.
Jung J, Nayak A, Schaeffer V, Starzetz T, Kirsch AK, Müller S, Dikic I, Mittelbronn M, Behrends C
eLife6(2017 Feb)

The C9orf72 GGGGCC repeat is translated into aggregating dipeptide-repeat proteins in FTLD/ALS.
Mori K, Weng SM, Arzberger T, May S, Rentzsch K, Kremmer E, Schmid B, Kretzschmar HA, Cruts M, Van Broeckhoven C, Haass C, Edbauer D
Science (New York, N.Y.)339(6125)1335-8(2013 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study found that the pattern of scores of three clinical measures distinguished between amyotrophic lateral sclerosis (ALS), ALS-familial frontotemporal dementia (FTD), or behavioral-variant FTD presentations of C9orf72 mutation carriers and asymptomatic carriers. Longitudinal changes in these measures occurred with disease progression in a manner consistent with presenting phenotype.
Title: Disease progression in

Study provides evidence for nucleolar stress in C9FTLD frontotemporal lobar degeneration patient brain, by using three-dimensional volumetric imaging; bidirectional changes in nucleolar volume dependent on the presence or absence of C9orf72 repeat RNA or protein pathologies show the heterogeneity of pathomechanisms in patient neurons
Title: Bidirectional nucleolar dysfunction in C9orf72 frontotemporal lobar degeneration.

This study demonstrated that C9orf72 hexanucleotide repeat expansions are not a common cause of obsessive-compulsive disorder.
Title: C9orf72 hexanucleotide repeat expansions are not a common cause of obsessive-compulsive disorder.

The C9orf72 hexanucleotide repeat expansion led to haploinsufficiency resulting in severely defective intracellular and extracellular vesicle trafficking
Title: C9orf72 and RAB7L1 regulate vesicle trafficking in amyotrophic lateral sclerosis and frontotemporal dementia.

Toxic C9orf72 dipeptide repeats change the arginine content and alter the properties of stress granules.
Title: Phase Separation of C9orf72 Dipeptide Repeats Perturbs Stress Granule Dynamics.

The following C9ORF72 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the C9ORF72 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu22712 NM_145005.6
Latest version!
Homo sapiens chromosome 9 open reading frame 72 (C9orf72), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu23067 NM_018325.4
Latest version!
Homo sapiens chromosome 9 open reading frame 72 (C9orf72), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu23067 NM_001256054.2
Latest version!
Homo sapiens chromosome 9 open reading frame 72 (C9orf72), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00

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Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
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All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu22712
Clone ID Related Accession (Same CDS sequence) NM_145005.6
Accession Version NM_145005.6 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 669bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-06
Organism Homo sapiens(Human)
Product protein C9orf72 isoform b
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BQ068108.1, AK057806.1, BC020851.2, DA802421.1 and AI141996.1. On Feb 7, 2015 this sequence version replaced NM_145005.5. Transcript Variant: This variant (1) lacks multiple exons in the central and 3' coding regions, and its 3' terminal exon extends beyond a splice site that is used in variant 3 . This results in a novel 3' UTR, compared to variant 3. It encodes isoform b which is significantly shorter, and its C-terminal amino acid is distinct, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK057806.1, SRR1803613.329188.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
ATGTCGACTC TTTGCCCACC GCCATCTCCA GCTGTTGCCA AGACAGAGAT TGCTTTAAGT 
GGCAAATCAC CTTTATTAGC AGCTACTTTT GCTTACTGGG ACAATATTCT TGGTCCTAGA
GTAAGGCACA TTTGGGCTCC AAAGACAGAA CAGGTACTTC TCAGTGATGG AGAAATAACT
TTTCTTGCCA ACCACACTCT AAATGGAGAA ATCCTTCGAA ATGCAGAGAG TGGTGCTATA
GATGTAAAGT TTTTTGTCTT GTCTGAAAAG GGAGTGATTA TTGTTTCATT AATCTTTGAT
GGAAACTGGA ATGGGGATCG CAGCACATAT GGACTATCAA TTATACTTCC ACAGACAGAA
CTTAGTTTCT ACCTCCCACT TCATAGAGTG TGTGTTGATA GATTAACACA TATAATCCGG
AAAGGAAGAA TATGGATGCA TAAGGAAAGA CAAGAAAATG TCCAGAAGAT TATCTTAGAA
GGCACAGAGA GAATGGAAGA TCAGGGTCAG AGTATTATTC CAATGCTTAC TGGAGAAGTG
ATTCCTGTAA TGGAACTGCT TTCATCTATG AAATCACACA GTGTTCCTGA AGAAATAGAT
ATAGCTGATA CAGTACTCAA TGATGATGAT ATTGGTGACA GCTGTCATGA AGGCTTTCTT
CTCAAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_659442.2
CDS125..793
Misc Feature(1)101..103
Misc Feature(2)305..>790
Exon (1)1..80
Exon (2)81..568
Exon (3)569..628
Exon (4)629..724
Exon (5)725..1950
Translation

Target ORF information:

RefSeq Version NM_145005.6
Organism Homo sapiens(Human)
Definition Homo sapiens chromosome 9 open reading frame 72 (C9orf72), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_145005.6

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGTCGACTC TTTGCCCACC GCCATCTCCA GCTGTTGCCA AGACAGAGAT TGCTTTAAGT 
GGCAAATCAC CTTTATTAGC AGCTACTTTT GCTTACTGGG ACAATATTCT TGGTCCTAGA
GTAAGGCACA TTTGGGCTCC AAAGACAGAA CAGGTACTTC TCAGTGATGG AGAAATAACT
TTTCTTGCCA ACCACACTCT AAATGGAGAA ATCCTTCGAA ATGCAGAGAG TGGTGCTATA
GATGTAAAGT TTTTTGTCTT GTCTGAAAAG GGAGTGATTA TTGTTTCATT AATCTTTGAT
GGAAACTGGA ATGGGGATCG CAGCACATAT GGACTATCAA TTATACTTCC ACAGACAGAA
CTTAGTTTCT ACCTCCCACT TCATAGAGTG TGTGTTGATA GATTAACACA TATAATCCGG
AAAGGAAGAA TATGGATGCA TAAGGAAAGA CAAGAAAATG TCCAGAAGAT TATCTTAGAA
GGCACAGAGA GAATGGAAGA TCAGGGTCAG AGTATTATTC CAATGCTTAC TGGAGAAGTG
ATTCCTGTAA TGGAACTGCT TTCATCTATG AAATCACACA GTGTTCCTGA AGAAATAGAT
ATAGCTGATA CAGTACTCAA TGATGATGAT ATTGGTGACA GCTGTCATGA AGGCTTTCTT
CTCAAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23067
Clone ID Related Accession (Same CDS sequence) NM_018325.4 , NM_001256054.2
Accession Version NM_018325.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1446bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-06
Organism Homo sapiens(Human)
Product protein C9orf72 isoform a
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG718065.1, AK291425.1, BC068445.1, BC020851.2, BC039112.1, BQ777139.1 and AL832229.1. On Feb 7, 2015 this sequence version replaced NM_018325.3. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 3. Both variants 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL832229.1, SRR1660805.35476.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGTCGACTC TTTGCCCACC GCCATCTCCA GCTGTTGCCA AGACAGAGAT TGCTTTAAGT 
GGCAAATCAC CTTTATTAGC AGCTACTTTT GCTTACTGGG ACAATATTCT TGGTCCTAGA
GTAAGGCACA TTTGGGCTCC AAAGACAGAA CAGGTACTTC TCAGTGATGG AGAAATAACT
TTTCTTGCCA ACCACACTCT AAATGGAGAA ATCCTTCGAA ATGCAGAGAG TGGTGCTATA
GATGTAAAGT TTTTTGTCTT GTCTGAAAAG GGAGTGATTA TTGTTTCATT AATCTTTGAT
GGAAACTGGA ATGGGGATCG CAGCACATAT GGACTATCAA TTATACTTCC ACAGACAGAA
CTTAGTTTCT ACCTCCCACT TCATAGAGTG TGTGTTGATA GATTAACACA TATAATCCGG
AAAGGAAGAA TATGGATGCA TAAGGAAAGA CAAGAAAATG TCCAGAAGAT TATCTTAGAA
GGCACAGAGA GAATGGAAGA TCAGGGTCAG AGTATTATTC CAATGCTTAC TGGAGAAGTG
ATTCCTGTAA TGGAACTGCT TTCATCTATG AAATCACACA GTGTTCCTGA AGAAATAGAT
ATAGCTGATA CAGTACTCAA TGATGATGAT ATTGGTGACA GCTGTCATGA AGGCTTTCTT
CTCAATGCCA TCAGCTCACA CTTGCAAACC TGTGGCTGTT CCGTTGTAGT AGGTAGCAGT
GCAGAGAAAG TAAATAAGAT AGTCAGAACA TTATGCCTTT TTCTGACTCC AGCAGAGAGA
AAATGCTCCA GGTTATGTGA AGCAGAATCA TCATTTAAAT ATGAGTCAGG GCTCTTTGTA
CAAGGCCTGC TAAAGGATTC AACTGGAAGC TTTGTGCTGC CTTTCCGGCA AGTCATGTAT
GCTCCATATC CCACCACACA CATAGATGTG GATGTCAATA CTGTGAAGCA GATGCCACCC
TGTCATGAAC ATATTTATAA TCAGCGTAGA TACATGAGAT CCGAGCTGAC AGCCTTCTGG
AGAGCCACTT CAGAAGAAGA CATGGCTCAG GATACGATCA TCTACACTGA CGAAAGCTTT
ACTCCTGATT TGAATATTTT TCAAGATGTC TTACACAGAG ACACTCTAGT GAAAGCCTTC
CTGGATCAGG TCTTTCAGCT GAAACCTGGC TTATCTCTCA GAAGTACTTT CCTTGCACAG
TTTCTACTTG TCCTTCACAG AAAAGCCTTG ACACTAATAA AATATATAGA AGACGATACG
CAGAAGGGAA AAAAGCCCTT TAAATCTCTT CGGAACCTGA AGATAGACCT TGATTTAACA
GCAGAGGGCG ATCTTAACAT AATAATGGCT CTGGCTGAGA AAATTAAACC AGGCCTACAC
TCTTTTATCT TTGGAAGACC TTTCTACACT AGTGTGCAAG AACGAGATGT TCTAATGACT
TTTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_060795.1
CDS108..1553
Misc Feature(1)84..86
Misc Feature(2)288..1079
Exon (1)1..63
Exon (2)64..551
Exon (3)552..611
Exon (4)612..707
Exon (5)708..772
Exon (6)773..845
Exon (7)846..962
Exon (8)963..1198
Exon (9)1199..1256
Exon (10)1257..1366
Exon (11)1367..3243
Translation

Target ORF information:

RefSeq Version NM_018325.4
Organism Homo sapiens(Human)
Definition Homo sapiens chromosome 9 open reading frame 72 (C9orf72), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_018325.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGTCGACTC TTTGCCCACC GCCATCTCCA GCTGTTGCCA AGACAGAGAT TGCTTTAAGT 
GGCAAATCAC CTTTATTAGC AGCTACTTTT GCTTACTGGG ACAATATTCT TGGTCCTAGA
GTAAGGCACA TTTGGGCTCC AAAGACAGAA CAGGTACTTC TCAGTGATGG AGAAATAACT
TTTCTTGCCA ACCACACTCT AAATGGAGAA ATCCTTCGAA ATGCAGAGAG TGGTGCTATA
GATGTAAAGT TTTTTGTCTT GTCTGAAAAG GGAGTGATTA TTGTTTCATT AATCTTTGAT
GGAAACTGGA ATGGGGATCG CAGCACATAT GGACTATCAA TTATACTTCC ACAGACAGAA
CTTAGTTTCT ACCTCCCACT TCATAGAGTG TGTGTTGATA GATTAACACA TATAATCCGG
AAAGGAAGAA TATGGATGCA TAAGGAAAGA CAAGAAAATG TCCAGAAGAT TATCTTAGAA
GGCACAGAGA GAATGGAAGA TCAGGGTCAG AGTATTATTC CAATGCTTAC TGGAGAAGTG
ATTCCTGTAA TGGAACTGCT TTCATCTATG AAATCACACA GTGTTCCTGA AGAAATAGAT
ATAGCTGATA CAGTACTCAA TGATGATGAT ATTGGTGACA GCTGTCATGA AGGCTTTCTT
CTCAATGCCA TCAGCTCACA CTTGCAAACC TGTGGCTGTT CCGTTGTAGT AGGTAGCAGT
GCAGAGAAAG TAAATAAGAT AGTCAGAACA TTATGCCTTT TTCTGACTCC AGCAGAGAGA
AAATGCTCCA GGTTATGTGA AGCAGAATCA TCATTTAAAT ATGAGTCAGG GCTCTTTGTA
CAAGGCCTGC TAAAGGATTC AACTGGAAGC TTTGTGCTGC CTTTCCGGCA AGTCATGTAT
GCTCCATATC CCACCACACA CATAGATGTG GATGTCAATA CTGTGAAGCA GATGCCACCC
TGTCATGAAC ATATTTATAA TCAGCGTAGA TACATGAGAT CCGAGCTGAC AGCCTTCTGG
AGAGCCACTT CAGAAGAAGA CATGGCTCAG GATACGATCA TCTACACTGA CGAAAGCTTT
ACTCCTGATT TGAATATTTT TCAAGATGTC TTACACAGAG ACACTCTAGT GAAAGCCTTC
CTGGATCAGG TCTTTCAGCT GAAACCTGGC TTATCTCTCA GAAGTACTTT CCTTGCACAG
TTTCTACTTG TCCTTCACAG AAAAGCCTTG ACACTAATAA AATATATAGA AGACGATACG
CAGAAGGGAA AAAAGCCCTT TAAATCTCTT CGGAACCTGA AGATAGACCT TGATTTAACA
GCAGAGGGCG ATCTTAACAT AATAATGGCT CTGGCTGAGA AAATTAAACC AGGCCTACAC
TCTTTTATCT TTGGAAGACC TTTCTACACT AGTGTGCAAG AACGAGATGT TCTAATGACT
TTTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23067
Clone ID Related Accession (Same CDS sequence) NM_018325.4 , NM_001256054.2
Accession Version NM_001256054.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1446bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-06
Organism Homo sapiens(Human)
Product protein C9orf72 isoform a
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BQ068108.1, JN681271.1 and AL832229.1. On Feb 7, 2015 this sequence version replaced NM_001256054.1. Transcript Variant: This variant (3) represents the longest transcript and encodes the longer isoform (a). Hexanucleotide expansions that can occur in a repeat region between the first and second exon of this variant have been associated with frontotemporal dementia and amyotrophic lateral sclerosis (FTD-ALS;PMID: 21944778, PMID: 21944779 ). Variants 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: JN681271.1, SRR1803611.127062.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGTCGACTC TTTGCCCACC GCCATCTCCA GCTGTTGCCA AGACAGAGAT TGCTTTAAGT 
GGCAAATCAC CTTTATTAGC AGCTACTTTT GCTTACTGGG ACAATATTCT TGGTCCTAGA
GTAAGGCACA TTTGGGCTCC AAAGACAGAA CAGGTACTTC TCAGTGATGG AGAAATAACT
TTTCTTGCCA ACCACACTCT AAATGGAGAA ATCCTTCGAA ATGCAGAGAG TGGTGCTATA
GATGTAAAGT TTTTTGTCTT GTCTGAAAAG GGAGTGATTA TTGTTTCATT AATCTTTGAT
GGAAACTGGA ATGGGGATCG CAGCACATAT GGACTATCAA TTATACTTCC ACAGACAGAA
CTTAGTTTCT ACCTCCCACT TCATAGAGTG TGTGTTGATA GATTAACACA TATAATCCGG
AAAGGAAGAA TATGGATGCA TAAGGAAAGA CAAGAAAATG TCCAGAAGAT TATCTTAGAA
GGCACAGAGA GAATGGAAGA TCAGGGTCAG AGTATTATTC CAATGCTTAC TGGAGAAGTG
ATTCCTGTAA TGGAACTGCT TTCATCTATG AAATCACACA GTGTTCCTGA AGAAATAGAT
ATAGCTGATA CAGTACTCAA TGATGATGAT ATTGGTGACA GCTGTCATGA AGGCTTTCTT
CTCAATGCCA TCAGCTCACA CTTGCAAACC TGTGGCTGTT CCGTTGTAGT AGGTAGCAGT
GCAGAGAAAG TAAATAAGAT AGTCAGAACA TTATGCCTTT TTCTGACTCC AGCAGAGAGA
AAATGCTCCA GGTTATGTGA AGCAGAATCA TCATTTAAAT ATGAGTCAGG GCTCTTTGTA
CAAGGCCTGC TAAAGGATTC AACTGGAAGC TTTGTGCTGC CTTTCCGGCA AGTCATGTAT
GCTCCATATC CCACCACACA CATAGATGTG GATGTCAATA CTGTGAAGCA GATGCCACCC
TGTCATGAAC ATATTTATAA TCAGCGTAGA TACATGAGAT CCGAGCTGAC AGCCTTCTGG
AGAGCCACTT CAGAAGAAGA CATGGCTCAG GATACGATCA TCTACACTGA CGAAAGCTTT
ACTCCTGATT TGAATATTTT TCAAGATGTC TTACACAGAG ACACTCTAGT GAAAGCCTTC
CTGGATCAGG TCTTTCAGCT GAAACCTGGC TTATCTCTCA GAAGTACTTT CCTTGCACAG
TTTCTACTTG TCCTTCACAG AAAAGCCTTG ACACTAATAA AATATATAGA AGACGATACG
CAGAAGGGAA AAAAGCCCTT TAAATCTCTT CGGAACCTGA AGATAGACCT TGATTTAACA
GCAGAGGGCG ATCTTAACAT AATAATGGCT CTGGCTGAGA AAATTAAACC AGGCCTACAC
TCTTTTATCT TTGGAAGACC TTTCTACACT AGTGTGCAAG AACGAGATGT TCTAATGACT
TTTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001242983.1
CDS203..1648
Misc Feature(1)179..181
Misc Feature(2)383..1174
Exon (1)1..158
Exon (2)159..646
Exon (3)647..706
Exon (4)707..802
Exon (5)803..867
Exon (6)868..940
Exon (7)941..1057
Exon (8)1058..1293
Exon (9)1294..1351
Exon (10)1352..1461
Exon (11)1462..3338
Translation

Target ORF information:

RefSeq Version NM_001256054.2
Organism Homo sapiens(Human)
Definition Homo sapiens chromosome 9 open reading frame 72 (C9orf72), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001256054.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGTCGACTC TTTGCCCACC GCCATCTCCA GCTGTTGCCA AGACAGAGAT TGCTTTAAGT 
GGCAAATCAC CTTTATTAGC AGCTACTTTT GCTTACTGGG ACAATATTCT TGGTCCTAGA
GTAAGGCACA TTTGGGCTCC AAAGACAGAA CAGGTACTTC TCAGTGATGG AGAAATAACT
TTTCTTGCCA ACCACACTCT AAATGGAGAA ATCCTTCGAA ATGCAGAGAG TGGTGCTATA
GATGTAAAGT TTTTTGTCTT GTCTGAAAAG GGAGTGATTA TTGTTTCATT AATCTTTGAT
GGAAACTGGA ATGGGGATCG CAGCACATAT GGACTATCAA TTATACTTCC ACAGACAGAA
CTTAGTTTCT ACCTCCCACT TCATAGAGTG TGTGTTGATA GATTAACACA TATAATCCGG
AAAGGAAGAA TATGGATGCA TAAGGAAAGA CAAGAAAATG TCCAGAAGAT TATCTTAGAA
GGCACAGAGA GAATGGAAGA TCAGGGTCAG AGTATTATTC CAATGCTTAC TGGAGAAGTG
ATTCCTGTAA TGGAACTGCT TTCATCTATG AAATCACACA GTGTTCCTGA AGAAATAGAT
ATAGCTGATA CAGTACTCAA TGATGATGAT ATTGGTGACA GCTGTCATGA AGGCTTTCTT
CTCAATGCCA TCAGCTCACA CTTGCAAACC TGTGGCTGTT CCGTTGTAGT AGGTAGCAGT
GCAGAGAAAG TAAATAAGAT AGTCAGAACA TTATGCCTTT TTCTGACTCC AGCAGAGAGA
AAATGCTCCA GGTTATGTGA AGCAGAATCA TCATTTAAAT ATGAGTCAGG GCTCTTTGTA
CAAGGCCTGC TAAAGGATTC AACTGGAAGC TTTGTGCTGC CTTTCCGGCA AGTCATGTAT
GCTCCATATC CCACCACACA CATAGATGTG GATGTCAATA CTGTGAAGCA GATGCCACCC
TGTCATGAAC ATATTTATAA TCAGCGTAGA TACATGAGAT CCGAGCTGAC AGCCTTCTGG
AGAGCCACTT CAGAAGAAGA CATGGCTCAG GATACGATCA TCTACACTGA CGAAAGCTTT
ACTCCTGATT TGAATATTTT TCAAGATGTC TTACACAGAG ACACTCTAGT GAAAGCCTTC
CTGGATCAGG TCTTTCAGCT GAAACCTGGC TTATCTCTCA GAAGTACTTT CCTTGCACAG
TTTCTACTTG TCCTTCACAG AAAAGCCTTG ACACTAATAA AATATATAGA AGACGATACG
CAGAAGGGAA AAAAGCCCTT TAAATCTCTT CGGAACCTGA AGATAGACCT TGATTTAACA
GCAGAGGGCG ATCTTAACAT AATAATGGCT CTGGCTGAGA AAATTAAACC AGGCCTACAC
TCTTTTATCT TTGGAAGACC TTTCTACACT AGTGTGCAAG AACGAGATGT TCTAATGACT
TTTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Bidirectional nucleolar dysfunction in C9orf72 frontotemporal lobar degeneration.
Acta neuropathologica communications5(1)29(2017 Apr)
Mizielinska S,Ridler CE,Balendra R,Thoeng A,Woodling NS,Grässer FA,Plagnol V,Lashley T,Partridge L,Isaacs AM


book

C9orf72 and RAB7L1 regulate vesicle trafficking in amyotrophic lateral sclerosis and frontotemporal dementia.
Brain : a journal of neurology140(4)887-897(2017 Apr)
Aoki Y,Manzano R,Lee Y,Dafinca R,Aoki M,Douglas AGL,Varela MA,Sathyaprakash C,Scaber J,Barbagallo P,Vader P,Mäger I,Ezzat K,Turner MR,Ito N,Gasco S,Ohbayashi N,El Andaloussi S,Takeda S,Fukuda M,Talbot K,Wood MJA


book

Multiplex image-based autophagy RNAi screening identifies SMCR8 as ULK1 kinase activity and gene expression regulator.
eLife6(2017 Feb)
Jung J,Nayak A,Schaeffer V,Starzetz T,Kirsch AK,Müller S,Dikic I,Mittelbronn M,Behrends C


book

The C9orf72 GGGGCC repeat is translated into aggregating dipeptide-repeat proteins in FTLD/ALS.
Science (New York, N.Y.)339(6125)1335-8(2013 Mar)
Mori K,Weng SM,Arzberger T,May S,Rentzsch K,Kremmer E,Schmid B,Kretzschmar HA,Cruts M,Van Broeckhoven C,Haass C,Edbauer D


 
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