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EPHB4 cDNA ORF clone, Homo sapiens (human)

Gene Symbol EPHB4
Entrez Gene ID 2050
Full Name EPH receptor B4
Synonyms HTK, MYK1, TYRO11
General protein information
Preferred Names
ephrin type-B receptor 4
Names
ephrin type-B receptor 4
ephrin receptor EphB4
soluble EPHB4 variant 1
soluble EPHB4 variant 2
soluble EPHB4 variant 3
hepatoma transmembrane kinase
tyrosine-protein kinase TYRO11
tyrosine-protein kinase receptor HTK
NP_004435.3
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

7

7q22

Summary Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene binds to ephrin-B2 and plays an essential role in vascular development. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

600011


mRNA and Protein(s)

mRNA Protein Name
NM_004444 NP_004435 ephrin type-B receptor 4 precursor



Homo sapiens (human) EPHB4 NP_004435.3
Pan troglodytes (chimpanzee) EPHB4 XP_519269.3
Macaca mulatta (Rhesus monkey) EPHB4 XP_002803271.1
Canis lupus familiaris (dog) EPHB4 XP_546948.3
Bos taurus (cattle) EPHB4 NP_001193197.1
Mus musculus (house mouse) Ephb4 NP_001153043.1
Rattus norvegicus (Norway rat) Ephb4 XP_003751205.1
Danio rerio (zebrafish) ephb4a NP_571489.1
Danio rerio (zebrafish) ephb4b NP_571492.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following EPHB4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EPHB4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu13402
NM_004444 Homo sapiens EPH receptor B4 (EPHB4), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$339.50-$475.30
$679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu13402
Clone ID Related Accession (Same CDS sequence) NM_004444
Accession Version NM_004444.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2964bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product ephrin type-B receptor 4 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB962032.1, BC052804.1 and AY056048.1. On Nov 16, 2004 this sequence version replaced gi:32528300. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC052804.1, U07695.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
ATGGAGCTCC GGGTGCTGCT CTGCTGGGCT TCGTTGGCCG CAGCTTTGGA AGAGACCCTG 
CTGAACACAA AATTGGAAAC TGCTGATCTG AAGTGGGTGA CATTCCCTCA GGTGGACGGG
CAGTGGGAGG AACTGAGCGG CCTGGATGAG GAACAGCACA GCGTGCGCAC CTACGAAGTG
TGTGACGTGC AGCGTGCCCC GGGCCAGGCC CACTGGCTTC GCACAGGTTG GGTCCCACGG
CGGGGCGCCG TCCACGTGTA CGCCACGCTG CGCTTCACCA TGCTCGAGTG CCTGTCCCTG
CCTCGGGCTG GGCGCTCCTG CAAGGAGACC TTCACCGTCT TCTACTATGA GAGCGATGCG
GACACGGCCA CGGCCCTCAC GCCAGCCTGG ATGGAGAACC CCTACATCAA GGTGGACACG
GTGGCCGCGG AGCATCTCAC CCGGAAGCGC CCTGGGGCCG AGGCCACCGG GAAGGTGAAT
GTCAAGACGC TGCGTCTGGG ACCGCTCAGC AAGGCTGGCT TCTACCTGGC CTTCCAGGAC
CAGGGTGCCT GCATGGCCCT GCTATCCCTG CACCTCTTCT ACAAAAAGTG CGCCCAGCTG
ACTGTGAACC TGACTCGATT CCCGGAGACT GTGCCTCGGG AGCTGGTTGT GCCCGTGGCC
GGTAGCTGCG TGGTGGATGC CGTCCCCGCC CCTGGCCCCA GCCCCAGCCT CTACTGCCGT
GAGGATGGCC AGTGGGCCGA ACAGCCGGTC ACGGGCTGCA GCTGTGCTCC GGGGTTCGAG
GCAGCTGAGG GGAACACCAA GTGCCGAGCC TGTGCCCAGG GCACCTTCAA GCCCCTGTCA
GGAGAAGGGT CCTGCCAGCC ATGCCCAGCC AATAGCCACT CTAACACCAT TGGATCAGCC
GTCTGCCAGT GCCGCGTCGG GTACTTCCGG GCACGCACAG ACCCCCGGGG TGCACCCTGC
ACCACCCCTC CTTCGGCTCC GCGGAGCGTG GTTTCCCGCC TGAACGGCTC CTCCCTGCAC
CTGGAATGGA GTGCCCCCCT GGAGTCTGGT GGCCGAGAGG ACCTCACCTA CGCCCTCCGC
TGCCGGGAGT GCCGACCCGG AGGCTCCTGT GCGCCCTGCG GGGGAGACCT GACTTTTGAC
CCCGGCCCCC GGGACCTGGT GGAGCCCTGG GTGGTGGTTC GAGGGCTACG TCCTGACTTC
ACCTATACCT TTGAGGTCAC TGCATTGAAC GGGGTATCCT CCTTAGCCAC GGGGCCCGTC
CCATTTGAGC CTGTCAATGT CACCACTGAC CGAGAGGTAC CTCCTGCAGT GTCTGACATC
CGGGTGACGC GGTCCTCACC CAGCAGCTTG AGCCTGGCCT GGGCTGTTCC CCGGGCACCC
AGTGGGGCTG TGCTGGACTA CGAGGTCAAA TACCATGAGA AGGGCGCCGA GGGTCCCAGC
AGCGTGCGGT TCCTGAAGAC GTCAGAAAAC CGGGCAGAGC TGCGGGGGCT GAAGCGGGGA
GCCAGCTACC TGGTGCAGGT ACGGGCGCGC TCTGAGGCCG GCTACGGGCC CTTCGGCCAG
GAACATCACA GCCAGACCCA ACTGGATGAG AGCGAGGGCT GGCGGGAGCA GCTGGCCCTG
ATTGCGGGCA CGGCAGTCGT GGGTGTGGTC CTGGTCCTGG TGGTCATTGT GGTCGCAGTT
CTCTGCCTCA GGAAGCAGAG CAATGGGAGA GAAGCAGAAT ATTCGGACAA ACACGGACAG
TATCTCATCG GACATGGTAC TAAGGTCTAC ATCGACCCCT TCACTTATGA AGACCCTAAT
GAGGCTGTGA GGGAATTTGC AAAAGAGATC GATGTCTCCT ACGTCAAGAT TGAAGAGGTG
ATTGGTGCAG GTGAGTTTGG CGAGGTGTGC CGGGGGCGGC TCAAGGCCCC AGGGAAGAAG
GAGAGCTGTG TGGCAATCAA GACCCTGAAG GGTGGCTACA CGGAGCGGCA GCGGCGTGAG
TTTCTGAGCG AGGCCTCCAT CATGGGCCAG TTCGAGCACC CCAATATCAT CCGCCTGGAG
GGCGTGGTCA CCAACAGCAT GCCCGTCATG ATTCTCACAG AGTTCATGGA GAACGGCGCC
CTGGACTCCT TCCTGCGGCT AAACGACGGA CAGTTCACAG TCATCCAGCT CGTGGGCATG
CTGCGGGGCA TCGCCTCGGG CATGCGGTAC CTTGCCGAGA TGAGCTACGT CCACCGAGAC
CTGGCTGCTC GCAACATCCT AGTCAACAGC AACCTCGTCT GCAAAGTGTC TGACTTTGGC
CTTTCCCGAT TCCTGGAGGA GAACTCTTCC GATCCCACCT ACACGAGCTC CCTGGGAGGA
AAGATTCCCA TCCGATGGAC TGCCCCGGAG GCCATTGCCT TCCGGAAGTT CACTTCCGCC
AGTGATGCCT GGAGTTACGG GATTGTGATG TGGGAGGTGA TGTCATTTGG GGAGAGGCCG
TACTGGGACA TGAGCAATCA GGACGTGATC AATGCCATTG AACAGGACTA CCGGCTGCCC
CCGCCCCCAG ACTGTCCCAC CTCCCTCCAC CAGCTCATGC TGGACTGTTG GCAGAAAGAC
CGGAATGCCC GGCCCCGCTT CCCCCAGGTG GTCAGCGCCC TGGACAAGAT GATCCGGAAC
CCCGCCAGCC TCAAAATCGT GGCCCGGGAG AATGGCGGGG CCTCACACCC TCTCCTGGAC
CAGCGGCAGC CTCACTACTC AGCTTTTGGC TCTGTGGGCG AGTGGCTTCG GGCCATCAAA
ATGGGAAGAT ACGAAGAAAG TTTCGCAGCC GCTGGCTTTG GCTCCTTCGA GCTGGTCAGC
CAGATCTCTG CTGAGGACCT GCTCCGAATC GGAGTCACTC TGGCGGGACA CCAGAAGAAA
ATCTTGGCCA GTGTCCAGCA CATGAAGTCC CAGGCCAAGC CGGGAACCCC GGGTGGGACA
GGAGGACCGG CCCCGCAGTA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_004435.3
CDS492..3455
Misc Feature(1)480..482(+)
Misc Feature(2)540..1079(+)
Misc Feature(3)570..1055(+)
Misc Feature(4)1293..>1454(+)
Misc Feature(5)1353..1454(+)
Misc Feature(6)1461..1778(+)
Misc Feature(7)1461..1730(+)
Misc Feature(8)1740..1757(+)
Misc Feature(9)1791..2069(+)
Misc Feature(10)1791..2033(+)
Misc Feature(11)2034..2048(+)
Misc Feature(12)2109..2171(+)
Misc Feature(13)2181..2327(+)
Misc Feature(14)2319..3125(+)
Misc Feature(15)2334..3113(+)
Misc Feature(16)2352..2969(+)
Misc Feature(17)2352..2765(+)
Misc Feature(18)2709..2969(+)
Misc Feature(19)2760..2846(+)
Misc Feature(20)3201..3404(+)
Misc Feature(21)3210..3410(+)
Misc Feature(22)3222..3224(+)
Misc Feature(23)3261..3362(+)
Misc Feature(24)3417..3419(+)
Misc Feature(25)3444..3452(+)
Misc Feature(26)3450..3452(+)
Exon (1)1..543
Gene:EPHB4
Gene Synonym:
Exon (2)544..614
Gene:EPHB4
Gene Synonym:
Exon (3)615..902
Gene:EPHB4
Gene Synonym:
Exon (4)903..1299
Gene:EPHB4
Gene Synonym:
Exon (5)1300..1455
Gene:EPHB4
Gene Synonym:
Exon (6)1456..1788
Gene:EPHB4
Gene Synonym:
Exon (7)1789..1913
Gene:EPHB4
Gene Synonym:
Exon (8)1914..2079
Gene:EPHB4
Gene Synonym:
Exon (9)2080..2182
Gene:EPHB4
Gene Synonym:
Exon (10)2183..2247
Gene:EPHB4
Gene Synonym:
Exon (11)2248..2361
Gene:EPHB4
Gene Synonym:
Exon (12)2362..2609
Gene:EPHB4
Gene Synonym:
Exon (13)2610..2825
Gene:EPHB4
Gene Synonym:
Exon (14)2826..2975
Gene:EPHB4
Gene Synonym:
Exon (15)2976..3169
Gene:EPHB4
Gene Synonym:
Exon (16)3170..3325
Gene:EPHB4
Gene Synonym:
Exon (17)3326..4351
Gene:EPHB4
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_004444
Organism Homo sapiens (human)
Definition Homo sapiens EPH receptor B4 (EPHB4), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_004444

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
ATGGAGCTCC GGGTGCTGCT CTGCTGGGCT TCGTTGGCCG CAGCTTTGGA AGAGACCCTG 
CTGAACACAA AATTGGAAAC TGCTGATCTG AAGTGGGTGA CATTCCCTCA GGTGGACGGG
CAGTGGGAGG AACTGAGCGG CCTGGATGAG GAACAGCACA GCGTGCGCAC CTACGAAGTG
TGTGACGTGC AGCGTGCCCC GGGCCAGGCC CACTGGCTTC GCACAGGTTG GGTCCCACGG
CGGGGCGCCG TCCACGTGTA CGCCACGCTG CGCTTCACCA TGCTCGAGTG CCTGTCCCTG
CCTCGGGCTG GGCGCTCCTG CAAGGAGACC TTCACCGTCT TCTACTATGA GAGCGATGCG
GACACGGCCA CGGCCCTCAC GCCAGCCTGG ATGGAGAACC CCTACATCAA GGTGGACACG
GTGGCCGCGG AGCATCTCAC CCGGAAGCGC CCTGGGGCCG AGGCCACCGG GAAGGTGAAT
GTCAAGACGC TGCGTCTGGG ACCGCTCAGC AAGGCTGGCT TCTACCTGGC CTTCCAGGAC
CAGGGTGCCT GCATGGCCCT GCTATCCCTG CACCTCTTCT ACAAAAAGTG CGCCCAGCTG
ACTGTGAACC TGACTCGATT CCCGGAGACT GTGCCTCGGG AGCTGGTTGT GCCCGTGGCC
GGTAGCTGCG TGGTGGATGC CGTCCCCGCC CCTGGCCCCA GCCCCAGCCT CTACTGCCGT
GAGGATGGCC AGTGGGCCGA ACAGCCGGTC ACGGGCTGCA GCTGTGCTCC GGGGTTCGAG
GCAGCTGAGG GGAACACCAA GTGCCGAGCC TGTGCCCAGG GCACCTTCAA GCCCCTGTCA
GGAGAAGGGT CCTGCCAGCC ATGCCCAGCC AATAGCCACT CTAACACCAT TGGATCAGCC
GTCTGCCAGT GCCGCGTCGG GTACTTCCGG GCACGCACAG ACCCCCGGGG TGCACCCTGC
ACCACCCCTC CTTCGGCTCC GCGGAGCGTG GTTTCCCGCC TGAACGGCTC CTCCCTGCAC
CTGGAATGGA GTGCCCCCCT GGAGTCTGGT GGCCGAGAGG ACCTCACCTA CGCCCTCCGC
TGCCGGGAGT GCCGACCCGG AGGCTCCTGT GCGCCCTGCG GGGGAGACCT GACTTTTGAC
CCCGGCCCCC GGGACCTGGT GGAGCCCTGG GTGGTGGTTC GAGGGCTACG TCCTGACTTC
ACCTATACCT TTGAGGTCAC TGCATTGAAC GGGGTATCCT CCTTAGCCAC GGGGCCCGTC
CCATTTGAGC CTGTCAATGT CACCACTGAC CGAGAGGTAC CTCCTGCAGT GTCTGACATC
CGGGTGACGC GGTCCTCACC CAGCAGCTTG AGCCTGGCCT GGGCTGTTCC CCGGGCACCC
AGTGGGGCTG TGCTGGACTA CGAGGTCAAA TACCATGAGA AGGGCGCCGA GGGTCCCAGC
AGCGTGCGGT TCCTGAAGAC GTCAGAAAAC CGGGCAGAGC TGCGGGGGCT GAAGCGGGGA
GCCAGCTACC TGGTGCAGGT ACGGGCGCGC TCTGAGGCCG GCTACGGGCC CTTCGGCCAG
GAACATCACA GCCAGACCCA ACTGGATGAG AGCGAGGGCT GGCGGGAGCA GCTGGCCCTG
ATTGCGGGCA CGGCAGTCGT GGGTGTGGTC CTGGTCCTGG TGGTCATTGT GGTCGCAGTT
CTCTGCCTCA GGAAGCAGAG CAATGGGAGA GAAGCAGAAT ATTCGGACAA ACACGGACAG
TATCTCATCG GACATGGTAC TAAGGTCTAC ATCGACCCCT TCACTTATGA AGACCCTAAT
GAGGCTGTGA GGGAATTTGC AAAAGAGATC GATGTCTCCT ACGTCAAGAT TGAAGAGGTG
ATTGGTGCAG GTGAGTTTGG CGAGGTGTGC CGGGGGCGGC TCAAGGCCCC AGGGAAGAAG
GAGAGCTGTG TGGCAATCAA GACCCTGAAG GGTGGCTACA CGGAGCGGCA GCGGCGTGAG
TTTCTGAGCG AGGCCTCCAT CATGGGCCAG TTCGAGCACC CCAATATCAT CCGCCTGGAG
GGCGTGGTCA CCAACAGCAT GCCCGTCATG ATTCTCACAG AGTTCATGGA GAACGGCGCC
CTGGACTCCT TCCTGCGGCT AAACGACGGA CAGTTCACAG TCATCCAGCT CGTGGGCATG
CTGCGGGGCA TCGCCTCGGG CATGCGGTAC CTTGCCGAGA TGAGCTACGT CCACCGAGAC
CTGGCTGCTC GCAACATCCT AGTCAACAGC AACCTCGTCT GCAAAGTGTC TGACTTTGGC
CTTTCCCGAT TCCTGGAGGA GAACTCTTCC GATCCCACCT ACACGAGCTC CCTGGGAGGA
AAGATTCCCA TCCGATGGAC TGCCCCGGAG GCCATTGCCT TCCGGAAGTT CACTTCCGCC
AGTGATGCCT GGAGTTACGG GATTGTGATG TGGGAGGTGA TGTCATTTGG GGAGAGGCCG
TACTGGGACA TGAGCAATCA GGACGTGATC AATGCCATTG AACAGGACTA CCGGCTGCCC
CCGCCCCCAG ACTGTCCCAC CTCCCTCCAC CAGCTCATGC TGGACTGTTG GCAGAAAGAC
CGGAATGCCC GGCCCCGCTT CCCCCAGGTG GTCAGCGCCC TGGACAAGAT GATCCGGAAC
CCCGCCAGCC TCAAAATCGT GGCCCGGGAG AATGGCGGGG CCTCACACCC TCTCCTGGAC
CAGCGGCAGC CTCACTACTC AGCTTTTGGC TCTGTGGGCG AGTGGCTTCG GGCCATCAAA
ATGGGAAGAT ACGAAGAAAG TTTCGCAGCC GCTGGCTTTG GCTCCTTCGA GCTGGTCAGC
CAGATCTCTG CTGAGGACCT GCTCCGAATC GGAGTCACTC TGGCGGGACA CCAGAAGAAA
ATCTTGGCCA GTGTCCAGCA CATGAAGTCC CAGGCCAAGC CGGGAACCCC GGGTGGGACA
GGAGGACCGG CCCCGCAGTA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Eph receptor B4 is a regulator of estrogen receptor alpha in breast cancer cells
J. Recept. Signal Transduct. Res. 33 (4), 244-248 (2013)
Schmitt F, Nguyen PH, Gupta N and Mayer D.


book

The EphB4 receptor tyrosine kinase promotes lung cancer growth: a potential novel therapeutic target
PLoS ONE 8 (7), E67668 (2013)
Ferguson BD, Liu R, Rolle CE, Tan YH, Krasnoperov V, Kanteti R, Tretiakova MS, Cervantes GM, Hasina R, Hseu RD, Iafrate AJ, Karrison T, Ferguson MK, Husain AN, Faoro L, Vokes EE, Gill PS and Salgia R.


book

Correlation between erythropoietin receptor(s) and estrogen and progesterone receptor expression in different breast cancer cell lines
Int. J. Mol. Med. 31 (3), 717-725 (2013)
Trost N, Hevir N, Rizner TL and Debeljak N.


book

EphB4 as a therapeutic target in mesothelioma
BMC Cancer 13, 269 (2013)
Liu R, Ferguson BD, Zhou Y, Naga K, Salgia R, Gill PS and Krasnoperov V.


book

Gene expression profiling identifies EPHB4 as a potential predictive biomarker in colorectal cancer patients treated with bevacizumab
Med. Oncol. 30 (2), 572 (2013)
Guijarro-Munoz I, Sanchez A, Martinez-Martinez E, Garcia JM, Salas C, Provencio M, Alvarez-Vallina L and Sanz L.


book

The Eph family receptors and ligands
Pharmacol. Ther. 77 (3), 151-181 (1998)
Zhou R.


book

The ephrins and Eph receptors in neural development
Annu. Rev. Neurosci. 21, 309-345 (1998)
Flanagan JG and Vanderhaeghen P.


book

Expression of the receptor protein tyrosine kinase myk-1/htk in normal and malignant mammary epithelium
Biochem. Biophys. Res. Commun. 226 (3), 869-875 (1996)
Berclaz G, Andres AC, Albrecht D, Dreher E, Ziemiecki A, Gusterson BA and Crompton MR.


book

Cloning and characterization of HTK, a novel transmembrane tyrosine kinase of the EPH subfamily
J. Biol. Chem. 269 (19), 14211-14218 (1994)
Bennett BD, Wang Z, Kuang WJ, Wang A, Groopman JE, Goeddel DV and Scadden DT.


book

Expression of two novel eph-related receptor protein tyrosine kinases in mammary gland development and carcinogenesis
Oncogene 9 (5), 1461-1467 (1994)
Andres AC, Reid HH, Zurcher G, Blaschke RJ, Albrecht D and Ziemiecki A.


 
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