SIRT1 cDNA ORF clone, Homo sapiens(human)

The following SIRT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SIRT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu26761 NM_001142498.1
Latest version!
Homo sapiens sirtuin 1 (SIRT1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu27002 NM_012238.5
Latest version!
Homo sapiens sirtuin 1 (SIRT1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
OHu44172 NM_001314049.1
Latest version!
Homo sapiens sirtuin 1 (SIRT1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
View Old Accession Versions >>
OHu27002 NM_012238.4 Homo sapiens sirtuin 1 (SIRT1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu26761
    Clone ID Related Accession (Same CDS sequence) NM_001142498.1
    Accession Version NM_001142498.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1359bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-31
    Organism Homo sapiens(human)
    Product NAD-dependent protein deacetylase sirtuin-1 isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK289743.1, DB162635.1, AA452304.1 and BC012499.1. Transcript Variant: This variant (2) contains an alternate in-frame exon in the 5' coding region and uses a downstream start codon, compared to variant 1. Isoform b has a shorter N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AL136741.1, SRR1660803.163777.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142670 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGTTTGATA TTGAATATTT CAGAAAAGAT CCAAGACCAT TCTTCAAGTT TGCAAAGGAA 
    ATATATCCTG GACAATTCCA GCCATCTCTC TGTCACAAAT TCATAGCCTT GTCAGATAAG
    GAAGGAAAAC TACTTCGCAA CTATACCCAG AACATAGACA CGCTGGAACA GGTTGCGGGA
    ATCCAAAGGA TAATTCAGTG TCATGGTTCC TTTGCAACAG CATCTTGCCT GATTTGTAAA
    TACAAAGTTG ACTGTGAAGC TGTACGAGGA GATATTTTTA ATCAGGTAGT TCCTCGATGT
    CCTAGGTGCC CAGCTGATGA ACCGCTTGCT ATCATGAAAC CAGAGATTGT GTTTTTTGGT
    GAAAATTTAC CAGAACAGTT TCATAGAGCC ATGAAGTATG ACAAAGATGA AGTTGACCTC
    CTCATTGTTA TTGGGTCTTC CCTCAAAGTA AGACCAGTAG CACTAATTCC AAGTTCCATA
    CCCCATGAAG TGCCTCAGAT ATTAATTAAT AGAGAACCTT TGCCTCATCT GCATTTTGAT
    GTAGAGCTTC TTGGAGACTG TGATGTCATA ATTAATGAAT TGTGTCATAG GTTAGGTGGT
    GAATATGCCA AACTTTGCTG TAACCCTGTA AAGCTTTCAG AAATTACTGA AAAACCTCCA
    CGAACACAAA AAGAATTGGC TTATTTGTCA GAGTTGCCAC CCACACCTCT TCATGTTTCA
    GAAGACTCAA GTTCACCAGA AAGAACTTCA CCACCAGATT CTTCAGTGAT TGTCACACTT
    TTAGACCAAG CAGCTAAGAG TAATGATGAT TTAGATGTGT CTGAATCAAA AGGTTGTATG
    GAAGAAAAAC CACAGGAAGT ACAAACTTCT AGGAATGTTG AAAGTATTGC TGAACAGATG
    GAAAATCCGG ATTTGAAGAA TGTTGGTTCT AGTACTGGGG AGAAAAATGA AAGAACTTCA
    GTGGCTGGAA CAGTGAGAAA ATGCTGGCCT AATAGAGTGG CAAAGGAGCA GATTAGTAGG
    CGGCTTGATG GTAATCAGTA TCTGTTTTTG CCACCAAATC GTTACATTTT CCATGGCGCT
    GAGGTATATT CAGACTCTGA AGATGACGTC TTATCCTCTA GTTCTTGTGG CAGTAACAGT
    GATAGTGGGA CATGCCAGAG TCCAAGTTTA GAAGAACCCA TGGAGGATGA AAGTGAAATT
    GAAGAATTCT ACAATGGCTT AGAAGATGAG CCTGATGTTC CAGAGAGAGC TGGAGGAGCT
    GGATTTGGGA CTGATGGAGA TGATCAAGAG GCAATTAATG AAGCTATATC TGTGAAACAG
    GAAGTAACAG ACATGAACTA TCCATCAAAC AAATCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001135970.1
    CDS433..1791
    Translation

    Target ORF information:

    RefSeq Version NM_001142498.1
    Organism Homo sapiens(human)
    Definition Homo sapiens sirtuin 1 (SIRT1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001142498.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGTTTGATA TTGAATATTT CAGAAAAGAT CCAAGACCAT TCTTCAAGTT TGCAAAGGAA 
    ATATATCCTG GACAATTCCA GCCATCTCTC TGTCACAAAT TCATAGCCTT GTCAGATAAG
    GAAGGAAAAC TACTTCGCAA CTATACCCAG AACATAGACA CGCTGGAACA GGTTGCGGGA
    ATCCAAAGGA TAATTCAGTG TCATGGTTCC TTTGCAACAG CATCTTGCCT GATTTGTAAA
    TACAAAGTTG ACTGTGAAGC TGTACGAGGA GATATTTTTA ATCAGGTAGT TCCTCGATGT
    CCTAGGTGCC CAGCTGATGA ACCGCTTGCT ATCATGAAAC CAGAGATTGT GTTTTTTGGT
    GAAAATTTAC CAGAACAGTT TCATAGAGCC ATGAAGTATG ACAAAGATGA AGTTGACCTC
    CTCATTGTTA TTGGGTCTTC CCTCAAAGTA AGACCAGTAG CACTAATTCC AAGTTCCATA
    CCCCATGAAG TGCCTCAGAT ATTAATTAAT AGAGAACCTT TGCCTCATCT GCATTTTGAT
    GTAGAGCTTC TTGGAGACTG TGATGTCATA ATTAATGAAT TGTGTCATAG GTTAGGTGGT
    GAATATGCCA AACTTTGCTG TAACCCTGTA AAGCTTTCAG AAATTACTGA AAAACCTCCA
    CGAACACAAA AAGAATTGGC TTATTTGTCA GAGTTGCCAC CCACACCTCT TCATGTTTCA
    GAAGACTCAA GTTCACCAGA AAGAACTTCA CCACCAGATT CTTCAGTGAT TGTCACACTT
    TTAGACCAAG CAGCTAAGAG TAATGATGAT TTAGATGTGT CTGAATCAAA AGGTTGTATG
    GAAGAAAAAC CACAGGAAGT ACAAACTTCT AGGAATGTTG AAAGTATTGC TGAACAGATG
    GAAAATCCGG ATTTGAAGAA TGTTGGTTCT AGTACTGGGG AGAAAAATGA AAGAACTTCA
    GTGGCTGGAA CAGTGAGAAA ATGCTGGCCT AATAGAGTGG CAAAGGAGCA GATTAGTAGG
    CGGCTTGATG GTAATCAGTA TCTGTTTTTG CCACCAAATC GTTACATTTT CCATGGCGCT
    GAGGTATATT CAGACTCTGA AGATGACGTC TTATCCTCTA GTTCTTGTGG CAGTAACAGT
    GATAGTGGGA CATGCCAGAG TCCAAGTTTA GAAGAACCCA TGGAGGATGA AAGTGAAATT
    GAAGAATTCT ACAATGGCTT AGAAGATGAG CCTGATGTTC CAGAGAGAGC TGGAGGAGCT
    GGATTTGGGA CTGATGGAGA TGATCAAGAG GCAATTAATG AAGCTATATC TGTGAAACAG
    GAAGTAACAG ACATGAACTA TCCATCAAAC AAATCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27002
    Clone ID Related Accession (Same CDS sequence) NM_012238.5 , NM_012238.4
    Accession Version NM_012238.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2244bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-01
    Organism Homo sapiens(human)
    Product NAD-dependent protein deacetylase sirtuin-1 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL133551.13. On Nov 23, 2018 this sequence version replaced NM_012238.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC012499.1, AF083106.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000212015.11/ ENSP00000212015.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCGGACG AGGCGGCCCT CGCCCTTCAG CCCGGCGGCT CCCCCTCGGC GGCGGGGGCC 
    GACAGGGAGG CCGCGTCGTC CCCCGCCGGG GAGCCGCTCC GCAAGAGGCC GCGGAGAGAT
    GGTCCCGGCC TCGAGCGGAG CCCGGGCGAG CCCGGTGGGG CGGCCCCAGA GCGTGAGGTG
    CCGGCGGCGG CCAGGGGCTG CCCGGGTGCG GCGGCGGCGG CGCTGTGGCG GGAGGCGGAG
    GCAGAGGCGG CGGCGGCAGG CGGGGAGCAA GAGGCCCAGG CGACTGCGGC GGCTGGGGAA
    GGAGACAATG GGCCGGGCCT GCAGGGCCCA TCTCGGGAGC CACCGCTGGC CGACAACTTG
    TACGACGAAG ACGACGACGA CGAGGGCGAG GAGGAGGAAG AGGCGGCGGC GGCGGCGATT
    GGGTACCGAG ATAACCTTCT GTTCGGTGAT GAAATTATCA CTAATGGTTT TCATTCCTGT
    GAAAGTGATG AGGAGGATAG AGCCTCACAT GCAAGCTCTA GTGACTGGAC TCCAAGGCCA
    CGGATAGGTC CATATACTTT TGTTCAGCAA CATCTTATGA TTGGCACAGA TCCTCGAACA
    ATTCTTAAAG ATTTATTGCC GGAAACAATA CCTCCACCTG AGTTGGATGA TATGACACTG
    TGGCAGATTG TTATTAATAT CCTTTCAGAA CCACCAAAAA GGAAAAAAAG AAAAGATATT
    AATACAATTG AAGATGCTGT GAAATTACTG CAAGAGTGCA AAAAAATTAT AGTTCTAACT
    GGAGCTGGGG TGTCTGTTTC ATGTGGAATA CCTGACTTCA GGTCAAGGGA TGGTATTTAT
    GCTCGCCTTG CTGTAGACTT CCCAGATCTT CCAGATCCTC AAGCGATGTT TGATATTGAA
    TATTTCAGAA AAGATCCAAG ACCATTCTTC AAGTTTGCAA AGGAAATATA TCCTGGACAA
    TTCCAGCCAT CTCTCTGTCA CAAATTCATA GCCTTGTCAG ATAAGGAAGG AAAACTACTT
    CGCAACTATA CCCAGAACAT AGACACGCTG GAACAGGTTG CGGGAATCCA AAGGATAATT
    CAGTGTCATG GTTCCTTTGC AACAGCATCT TGCCTGATTT GTAAATACAA AGTTGACTGT
    GAAGCTGTAC GAGGAGATAT TTTTAATCAG GTAGTTCCTC GATGTCCTAG GTGCCCAGCT
    GATGAACCGC TTGCTATCAT GAAACCAGAG ATTGTGTTTT TTGGTGAAAA TTTACCAGAA
    CAGTTTCATA GAGCCATGAA GTATGACAAA GATGAAGTTG ACCTCCTCAT TGTTATTGGG
    TCTTCCCTCA AAGTAAGACC AGTAGCACTA ATTCCAAGTT CCATACCCCA TGAAGTGCCT
    CAGATATTAA TTAATAGAGA ACCTTTGCCT CATCTGCATT TTGATGTAGA GCTTCTTGGA
    GACTGTGATG TCATAATTAA TGAATTGTGT CATAGGTTAG GTGGTGAATA TGCCAAACTT
    TGCTGTAACC CTGTAAAGCT TTCAGAAATT ACTGAAAAAC CTCCACGAAC ACAAAAAGAA
    TTGGCTTATT TGTCAGAGTT GCCACCCACA CCTCTTCATG TTTCAGAAGA CTCAAGTTCA
    CCAGAAAGAA CTTCACCACC AGATTCTTCA GTGATTGTCA CACTTTTAGA CCAAGCAGCT
    AAGAGTAATG ATGATTTAGA TGTGTCTGAA TCAAAAGGTT GTATGGAAGA AAAACCACAG
    GAAGTACAAA CTTCTAGGAA TGTTGAAAGT ATTGCTGAAC AGATGGAAAA TCCGGATTTG
    AAGAATGTTG GTTCTAGTAC TGGGGAGAAA AATGAAAGAA CTTCAGTGGC TGGAACAGTG
    AGAAAATGCT GGCCTAATAG AGTGGCAAAG GAGCAGATTA GTAGGCGGCT TGATGGTAAT
    CAGTATCTGT TTTTGCCACC AAATCGTTAC ATTTTCCATG GCGCTGAGGT ATATTCAGAC
    TCTGAAGATG ACGTCTTATC CTCTAGTTCT TGTGGCAGTA ACAGTGATAG TGGGACATGC
    CAGAGTCCAA GTTTAGAAGA ACCCATGGAG GATGAAAGTG AAATTGAAGA ATTCTACAAT
    GGCTTAGAAG ATGAGCCTGA TGTTCCAGAG AGAGCTGGAG GAGCTGGATT TGGGACTGAT
    GGAGATGATC AAGAGGCAAT TAATGAAGCT ATATCTGTGA AACAGGAAGT AACAGACATG
    AACTATCCAT CAAACAAATC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_036370.2
    CDS67..2310
    Translation

    Target ORF information:

    RefSeq Version NM_012238.5
    Organism Homo sapiens(human)
    Definition Homo sapiens sirtuin 1 (SIRT1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_012238.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCGGACG AGGCGGCCCT CGCCCTTCAG CCCGGCGGCT CCCCCTCGGC GGCGGGGGCC 
    GACAGGGAGG CCGCGTCGTC CCCCGCCGGG GAGCCGCTCC GCAAGAGGCC GCGGAGAGAT
    GGTCCCGGCC TCGAGCGGAG CCCGGGCGAG CCCGGTGGGG CGGCCCCAGA GCGTGAGGTG
    CCGGCGGCGG CCAGGGGCTG CCCGGGTGCG GCGGCGGCGG CGCTGTGGCG GGAGGCGGAG
    GCAGAGGCGG CGGCGGCAGG CGGGGAGCAA GAGGCCCAGG CGACTGCGGC GGCTGGGGAA
    GGAGACAATG GGCCGGGCCT GCAGGGCCCA TCTCGGGAGC CACCGCTGGC CGACAACTTG
    TACGACGAAG ACGACGACGA CGAGGGCGAG GAGGAGGAAG AGGCGGCGGC GGCGGCGATT
    GGGTACCGAG ATAACCTTCT GTTCGGTGAT GAAATTATCA CTAATGGTTT TCATTCCTGT
    GAAAGTGATG AGGAGGATAG AGCCTCACAT GCAAGCTCTA GTGACTGGAC TCCAAGGCCA
    CGGATAGGTC CATATACTTT TGTTCAGCAA CATCTTATGA TTGGCACAGA TCCTCGAACA
    ATTCTTAAAG ATTTATTGCC GGAAACAATA CCTCCACCTG AGTTGGATGA TATGACACTG
    TGGCAGATTG TTATTAATAT CCTTTCAGAA CCACCAAAAA GGAAAAAAAG AAAAGATATT
    AATACAATTG AAGATGCTGT GAAATTACTG CAAGAGTGCA AAAAAATTAT AGTTCTAACT
    GGAGCTGGGG TGTCTGTTTC ATGTGGAATA CCTGACTTCA GGTCAAGGGA TGGTATTTAT
    GCTCGCCTTG CTGTAGACTT CCCAGATCTT CCAGATCCTC AAGCGATGTT TGATATTGAA
    TATTTCAGAA AAGATCCAAG ACCATTCTTC AAGTTTGCAA AGGAAATATA TCCTGGACAA
    TTCCAGCCAT CTCTCTGTCA CAAATTCATA GCCTTGTCAG ATAAGGAAGG AAAACTACTT
    CGCAACTATA CCCAGAACAT AGACACGCTG GAACAGGTTG CGGGAATCCA AAGGATAATT
    CAGTGTCATG GTTCCTTTGC AACAGCATCT TGCCTGATTT GTAAATACAA AGTTGACTGT
    GAAGCTGTAC GAGGAGATAT TTTTAATCAG GTAGTTCCTC GATGTCCTAG GTGCCCAGCT
    GATGAACCGC TTGCTATCAT GAAACCAGAG ATTGTGTTTT TTGGTGAAAA TTTACCAGAA
    CAGTTTCATA GAGCCATGAA GTATGACAAA GATGAAGTTG ACCTCCTCAT TGTTATTGGG
    TCTTCCCTCA AAGTAAGACC AGTAGCACTA ATTCCAAGTT CCATACCCCA TGAAGTGCCT
    CAGATATTAA TTAATAGAGA ACCTTTGCCT CATCTGCATT TTGATGTAGA GCTTCTTGGA
    GACTGTGATG TCATAATTAA TGAATTGTGT CATAGGTTAG GTGGTGAATA TGCCAAACTT
    TGCTGTAACC CTGTAAAGCT TTCAGAAATT ACTGAAAAAC CTCCACGAAC ACAAAAAGAA
    TTGGCTTATT TGTCAGAGTT GCCACCCACA CCTCTTCATG TTTCAGAAGA CTCAAGTTCA
    CCAGAAAGAA CTTCACCACC AGATTCTTCA GTGATTGTCA CACTTTTAGA CCAAGCAGCT
    AAGAGTAATG ATGATTTAGA TGTGTCTGAA TCAAAAGGTT GTATGGAAGA AAAACCACAG
    GAAGTACAAA CTTCTAGGAA TGTTGAAAGT ATTGCTGAAC AGATGGAAAA TCCGGATTTG
    AAGAATGTTG GTTCTAGTAC TGGGGAGAAA AATGAAAGAA CTTCAGTGGC TGGAACAGTG
    AGAAAATGCT GGCCTAATAG AGTGGCAAAG GAGCAGATTA GTAGGCGGCT TGATGGTAAT
    CAGTATCTGT TTTTGCCACC AAATCGTTAC ATTTTCCATG GCGCTGAGGT ATATTCAGAC
    TCTGAAGATG ACGTCTTATC CTCTAGTTCT TGTGGCAGTA ACAGTGATAG TGGGACATGC
    CAGAGTCCAA GTTTAGAAGA ACCCATGGAG GATGAAAGTG AAATTGAAGA ATTCTACAAT
    GGCTTAGAAG ATGAGCCTGA TGTTCCAGAG AGAGCTGGAG GAGCTGGATT TGGGACTGAT
    GGAGATGATC AAGAGGCAAT TAATGAAGCT ATATCTGTGA AACAGGAAGT AACAGACATG
    AACTATCCAT CAAACAAATC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27002
    Clone ID Related Accession (Same CDS sequence) NM_012238.5 , NM_012238.4
    Accession Version NM_012238.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2244bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-05
    Organism Homo sapiens(human)
    Product NAD-dependent protein deacetylase sirtuin-1 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF083106.2, AA452304.1 and BC012499.1. This sequence is a reference standard in the RefSeqGene project. On Dec 5, 2008 this sequence version replaced NM_012238.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF083106.2, AL136741.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCGGACG AGGCGGCCCT CGCCCTTCAG CCCGGCGGCT CCCCCTCGGC GGCGGGGGCC 
    GACAGGGAGG CCGCGTCGTC CCCCGCCGGG GAGCCGCTCC GCAAGAGGCC GCGGAGAGAT
    GGTCCCGGCC TCGAGCGGAG CCCGGGCGAG CCCGGTGGGG CGGCCCCAGA GCGTGAGGTG
    CCGGCGGCGG CCAGGGGCTG CCCGGGTGCG GCGGCGGCGG CGCTGTGGCG GGAGGCGGAG
    GCAGAGGCGG CGGCGGCAGG CGGGGAGCAA GAGGCCCAGG CGACTGCGGC GGCTGGGGAA
    GGAGACAATG GGCCGGGCCT GCAGGGCCCA TCTCGGGAGC CACCGCTGGC CGACAACTTG
    TACGACGAAG ACGACGACGA CGAGGGCGAG GAGGAGGAAG AGGCGGCGGC GGCGGCGATT
    GGGTACCGAG ATAACCTTCT GTTCGGTGAT GAAATTATCA CTAATGGTTT TCATTCCTGT
    GAAAGTGATG AGGAGGATAG AGCCTCACAT GCAAGCTCTA GTGACTGGAC TCCAAGGCCA
    CGGATAGGTC CATATACTTT TGTTCAGCAA CATCTTATGA TTGGCACAGA TCCTCGAACA
    ATTCTTAAAG ATTTATTGCC GGAAACAATA CCTCCACCTG AGTTGGATGA TATGACACTG
    TGGCAGATTG TTATTAATAT CCTTTCAGAA CCACCAAAAA GGAAAAAAAG AAAAGATATT
    AATACAATTG AAGATGCTGT GAAATTACTG CAAGAGTGCA AAAAAATTAT AGTTCTAACT
    GGAGCTGGGG TGTCTGTTTC ATGTGGAATA CCTGACTTCA GGTCAAGGGA TGGTATTTAT
    GCTCGCCTTG CTGTAGACTT CCCAGATCTT CCAGATCCTC AAGCGATGTT TGATATTGAA
    TATTTCAGAA AAGATCCAAG ACCATTCTTC AAGTTTGCAA AGGAAATATA TCCTGGACAA
    TTCCAGCCAT CTCTCTGTCA CAAATTCATA GCCTTGTCAG ATAAGGAAGG AAAACTACTT
    CGCAACTATA CCCAGAACAT AGACACGCTG GAACAGGTTG CGGGAATCCA AAGGATAATT
    CAGTGTCATG GTTCCTTTGC AACAGCATCT TGCCTGATTT GTAAATACAA AGTTGACTGT
    GAAGCTGTAC GAGGAGATAT TTTTAATCAG GTAGTTCCTC GATGTCCTAG GTGCCCAGCT
    GATGAACCGC TTGCTATCAT GAAACCAGAG ATTGTGTTTT TTGGTGAAAA TTTACCAGAA
    CAGTTTCATA GAGCCATGAA GTATGACAAA GATGAAGTTG ACCTCCTCAT TGTTATTGGG
    TCTTCCCTCA AAGTAAGACC AGTAGCACTA ATTCCAAGTT CCATACCCCA TGAAGTGCCT
    CAGATATTAA TTAATAGAGA ACCTTTGCCT CATCTGCATT TTGATGTAGA GCTTCTTGGA
    GACTGTGATG TCATAATTAA TGAATTGTGT CATAGGTTAG GTGGTGAATA TGCCAAACTT
    TGCTGTAACC CTGTAAAGCT TTCAGAAATT ACTGAAAAAC CTCCACGAAC ACAAAAAGAA
    TTGGCTTATT TGTCAGAGTT GCCACCCACA CCTCTTCATG TTTCAGAAGA CTCAAGTTCA
    CCAGAAAGAA CTTCACCACC AGATTCTTCA GTGATTGTCA CACTTTTAGA CCAAGCAGCT
    AAGAGTAATG ATGATTTAGA TGTGTCTGAA TCAAAAGGTT GTATGGAAGA AAAACCACAG
    GAAGTACAAA CTTCTAGGAA TGTTGAAAGT ATTGCTGAAC AGATGGAAAA TCCGGATTTG
    AAGAATGTTG GTTCTAGTAC TGGGGAGAAA AATGAAAGAA CTTCAGTGGC TGGAACAGTG
    AGAAAATGCT GGCCTAATAG AGTGGCAAAG GAGCAGATTA GTAGGCGGCT TGATGGTAAT
    CAGTATCTGT TTTTGCCACC AAATCGTTAC ATTTTCCATG GCGCTGAGGT ATATTCAGAC
    TCTGAAGATG ACGTCTTATC CTCTAGTTCT TGTGGCAGTA ACAGTGATAG TGGGACATGC
    CAGAGTCCAA GTTTAGAAGA ACCCATGGAG GATGAAAGTG AAATTGAAGA ATTCTACAAT
    GGCTTAGAAG ATGAGCCTGA TGTTCCAGAG AGAGCTGGAG GAGCTGGATT TGGGACTGAT
    GGAGATGATC AAGAGGCAAT TAATGAAGCT ATATCTGTGA AACAGGAAGT AACAGACATG
    AACTATCCAT CAAACAAATC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_036370.2
    CDS54..2297
    Translation

    Target ORF information:

    RefSeq Version NM_012238.4
    Organism Homo sapiens(human)
    Definition Homo sapiens sirtuin 1 (SIRT1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_012238.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCGGACG AGGCGGCCCT CGCCCTTCAG CCCGGCGGCT CCCCCTCGGC GGCGGGGGCC 
    GACAGGGAGG CCGCGTCGTC CCCCGCCGGG GAGCCGCTCC GCAAGAGGCC GCGGAGAGAT
    GGTCCCGGCC TCGAGCGGAG CCCGGGCGAG CCCGGTGGGG CGGCCCCAGA GCGTGAGGTG
    CCGGCGGCGG CCAGGGGCTG CCCGGGTGCG GCGGCGGCGG CGCTGTGGCG GGAGGCGGAG
    GCAGAGGCGG CGGCGGCAGG CGGGGAGCAA GAGGCCCAGG CGACTGCGGC GGCTGGGGAA
    GGAGACAATG GGCCGGGCCT GCAGGGCCCA TCTCGGGAGC CACCGCTGGC CGACAACTTG
    TACGACGAAG ACGACGACGA CGAGGGCGAG GAGGAGGAAG AGGCGGCGGC GGCGGCGATT
    GGGTACCGAG ATAACCTTCT GTTCGGTGAT GAAATTATCA CTAATGGTTT TCATTCCTGT
    GAAAGTGATG AGGAGGATAG AGCCTCACAT GCAAGCTCTA GTGACTGGAC TCCAAGGCCA
    CGGATAGGTC CATATACTTT TGTTCAGCAA CATCTTATGA TTGGCACAGA TCCTCGAACA
    ATTCTTAAAG ATTTATTGCC GGAAACAATA CCTCCACCTG AGTTGGATGA TATGACACTG
    TGGCAGATTG TTATTAATAT CCTTTCAGAA CCACCAAAAA GGAAAAAAAG AAAAGATATT
    AATACAATTG AAGATGCTGT GAAATTACTG CAAGAGTGCA AAAAAATTAT AGTTCTAACT
    GGAGCTGGGG TGTCTGTTTC ATGTGGAATA CCTGACTTCA GGTCAAGGGA TGGTATTTAT
    GCTCGCCTTG CTGTAGACTT CCCAGATCTT CCAGATCCTC AAGCGATGTT TGATATTGAA
    TATTTCAGAA AAGATCCAAG ACCATTCTTC AAGTTTGCAA AGGAAATATA TCCTGGACAA
    TTCCAGCCAT CTCTCTGTCA CAAATTCATA GCCTTGTCAG ATAAGGAAGG AAAACTACTT
    CGCAACTATA CCCAGAACAT AGACACGCTG GAACAGGTTG CGGGAATCCA AAGGATAATT
    CAGTGTCATG GTTCCTTTGC AACAGCATCT TGCCTGATTT GTAAATACAA AGTTGACTGT
    GAAGCTGTAC GAGGAGATAT TTTTAATCAG GTAGTTCCTC GATGTCCTAG GTGCCCAGCT
    GATGAACCGC TTGCTATCAT GAAACCAGAG ATTGTGTTTT TTGGTGAAAA TTTACCAGAA
    CAGTTTCATA GAGCCATGAA GTATGACAAA GATGAAGTTG ACCTCCTCAT TGTTATTGGG
    TCTTCCCTCA AAGTAAGACC AGTAGCACTA ATTCCAAGTT CCATACCCCA TGAAGTGCCT
    CAGATATTAA TTAATAGAGA ACCTTTGCCT CATCTGCATT TTGATGTAGA GCTTCTTGGA
    GACTGTGATG TCATAATTAA TGAATTGTGT CATAGGTTAG GTGGTGAATA TGCCAAACTT
    TGCTGTAACC CTGTAAAGCT TTCAGAAATT ACTGAAAAAC CTCCACGAAC ACAAAAAGAA
    TTGGCTTATT TGTCAGAGTT GCCACCCACA CCTCTTCATG TTTCAGAAGA CTCAAGTTCA
    CCAGAAAGAA CTTCACCACC AGATTCTTCA GTGATTGTCA CACTTTTAGA CCAAGCAGCT
    AAGAGTAATG ATGATTTAGA TGTGTCTGAA TCAAAAGGTT GTATGGAAGA AAAACCACAG
    GAAGTACAAA CTTCTAGGAA TGTTGAAAGT ATTGCTGAAC AGATGGAAAA TCCGGATTTG
    AAGAATGTTG GTTCTAGTAC TGGGGAGAAA AATGAAAGAA CTTCAGTGGC TGGAACAGTG
    AGAAAATGCT GGCCTAATAG AGTGGCAAAG GAGCAGATTA GTAGGCGGCT TGATGGTAAT
    CAGTATCTGT TTTTGCCACC AAATCGTTAC ATTTTCCATG GCGCTGAGGT ATATTCAGAC
    TCTGAAGATG ACGTCTTATC CTCTAGTTCT TGTGGCAGTA ACAGTGATAG TGGGACATGC
    CAGAGTCCAA GTTTAGAAGA ACCCATGGAG GATGAAAGTG AAATTGAAGA ATTCTACAAT
    GGCTTAGAAG ATGAGCCTGA TGTTCCAGAG AGAGCTGGAG GAGCTGGATT TGGGACTGAT
    GGAGATGATC AAGAGGCAAT TAATGAAGCT ATATCTGTGA AACAGGAAGT AACAGACATG
    AACTATCCAT CAAACAAATC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu44172
    Clone ID Related Accession (Same CDS sequence) NM_001314049.1
    Accession Version NM_001314049.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1335bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-01
    Organism Homo sapiens(human)
    Product NAD-dependent protein deacetylase sirtuin-1 isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB162635.1, AK074805.1, AF235040.1 and BC012499.1. On Sep 29, 2015 this sequence version replaced XM_005269663.1. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region and uses an alternate translation start site compared to variant 1. The encoded isoform (c) is shorter and has a distinct N-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK074805.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGTGCCTGT GCAGTGGAAG GAAAACAATT TTGGAAATAT ATCCTGGACA ATTCCAGCCA 
    TCTCTCTGTC ACAAATTCAT AGCCTTGTCA GATAAGGAAG GAAAACTACT TCGCAACTAT
    ACCCAGAACA TAGACACGCT GGAACAGGTT GCGGGAATCC AAAGGATAAT TCAGTGTCAT
    GGTTCCTTTG CAACAGCATC TTGCCTGATT TGTAAATACA AAGTTGACTG TGAAGCTGTA
    CGAGGAGATA TTTTTAATCA GGTAGTTCCT CGATGTCCTA GGTGCCCAGC TGATGAACCG
    CTTGCTATCA TGAAACCAGA GATTGTGTTT TTTGGTGAAA ATTTACCAGA ACAGTTTCAT
    AGAGCCATGA AGTATGACAA AGATGAAGTT GACCTCCTCA TTGTTATTGG GTCTTCCCTC
    AAAGTAAGAC CAGTAGCACT AATTCCAAGT TCCATACCCC ATGAAGTGCC TCAGATATTA
    ATTAATAGAG AACCTTTGCC TCATCTGCAT TTTGATGTAG AGCTTCTTGG AGACTGTGAT
    GTCATAATTA ATGAATTGTG TCATAGGTTA GGTGGTGAAT ATGCCAAACT TTGCTGTAAC
    CCTGTAAAGC TTTCAGAAAT TACTGAAAAA CCTCCACGAA CACAAAAAGA ATTGGCTTAT
    TTGTCAGAGT TGCCACCCAC ACCTCTTCAT GTTTCAGAAG ACTCAAGTTC ACCAGAAAGA
    ACTTCACCAC CAGATTCTTC AGTGATTGTC ACACTTTTAG ACCAAGCAGC TAAGAGTAAT
    GATGATTTAG ATGTGTCTGA ATCAAAAGGT TGTATGGAAG AAAAACCACA GGAAGTACAA
    ACTTCTAGGA ATGTTGAAAG TATTGCTGAA CAGATGGAAA ATCCGGATTT GAAGAATGTT
    GGTTCTAGTA CTGGGGAGAA AAATGAAAGA ACTTCAGTGG CTGGAACAGT GAGAAAATGC
    TGGCCTAATA GAGTGGCAAA GGAGCAGATT AGTAGGCGGC TTGATGGTAA TCAGTATCTG
    TTTTTGCCAC CAAATCGTTA CATTTTCCAT GGCGCTGAGG TATATTCAGA CTCTGAAGAT
    GACGTCTTAT CCTCTAGTTC TTGTGGCAGT AACAGTGATA GTGGGACATG CCAGAGTCCA
    AGTTTAGAAG AACCCATGGA GGATGAAAGT GAAATTGAAG AATTCTACAA TGGCTTAGAA
    GATGAGCCTG ATGTTCCAGA GAGAGCTGGA GGAGCTGGAT TTGGGACTGA TGGAGATGAT
    CAAGAGGCAA TTAATGAAGC TATATCTGTG AAACAGGAAG TAACAGACAT GAACTATCCA
    TCAAACAAAT CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001300978.1
    CDS246..1580
    Translation

    Target ORF information:

    RefSeq Version NM_001314049.1
    Organism Homo sapiens(human)
    Definition Homo sapiens sirtuin 1 (SIRT1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001314049.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGTGCCTGT GCAGTGGAAG GAAAACAATT TTGGAAATAT ATCCTGGACA ATTCCAGCCA 
    TCTCTCTGTC ACAAATTCAT AGCCTTGTCA GATAAGGAAG GAAAACTACT TCGCAACTAT
    ACCCAGAACA TAGACACGCT GGAACAGGTT GCGGGAATCC AAAGGATAAT TCAGTGTCAT
    GGTTCCTTTG CAACAGCATC TTGCCTGATT TGTAAATACA AAGTTGACTG TGAAGCTGTA
    CGAGGAGATA TTTTTAATCA GGTAGTTCCT CGATGTCCTA GGTGCCCAGC TGATGAACCG
    CTTGCTATCA TGAAACCAGA GATTGTGTTT TTTGGTGAAA ATTTACCAGA ACAGTTTCAT
    AGAGCCATGA AGTATGACAA AGATGAAGTT GACCTCCTCA TTGTTATTGG GTCTTCCCTC
    AAAGTAAGAC CAGTAGCACT AATTCCAAGT TCCATACCCC ATGAAGTGCC TCAGATATTA
    ATTAATAGAG AACCTTTGCC TCATCTGCAT TTTGATGTAG AGCTTCTTGG AGACTGTGAT
    GTCATAATTA ATGAATTGTG TCATAGGTTA GGTGGTGAAT ATGCCAAACT TTGCTGTAAC
    CCTGTAAAGC TTTCAGAAAT TACTGAAAAA CCTCCACGAA CACAAAAAGA ATTGGCTTAT
    TTGTCAGAGT TGCCACCCAC ACCTCTTCAT GTTTCAGAAG ACTCAAGTTC ACCAGAAAGA
    ACTTCACCAC CAGATTCTTC AGTGATTGTC ACACTTTTAG ACCAAGCAGC TAAGAGTAAT
    GATGATTTAG ATGTGTCTGA ATCAAAAGGT TGTATGGAAG AAAAACCACA GGAAGTACAA
    ACTTCTAGGA ATGTTGAAAG TATTGCTGAA CAGATGGAAA ATCCGGATTT GAAGAATGTT
    GGTTCTAGTA CTGGGGAGAA AAATGAAAGA ACTTCAGTGG CTGGAACAGT GAGAAAATGC
    TGGCCTAATA GAGTGGCAAA GGAGCAGATT AGTAGGCGGC TTGATGGTAA TCAGTATCTG
    TTTTTGCCAC CAAATCGTTA CATTTTCCAT GGCGCTGAGG TATATTCAGA CTCTGAAGAT
    GACGTCTTAT CCTCTAGTTC TTGTGGCAGT AACAGTGATA GTGGGACATG CCAGAGTCCA
    AGTTTAGAAG AACCCATGGA GGATGAAAGT GAAATTGAAG AATTCTACAA TGGCTTAGAA
    GATGAGCCTG ATGTTCCAGA GAGAGCTGGA GGAGCTGGAT TTGGGACTGA TGGAGATGAT
    CAAGAGGCAA TTAATGAAGC TATATCTGTG AAACAGGAAG TAACAGACAT GAACTATCCA
    TCAAACAAAT CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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