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SIRT1 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol SIRT1
Entrez Gene ID 23411
Full Name sirtuin 1
Synonyms SIR2,SIR2L1,SIR2alpha
General protein information
Preferred Names

sirtuin 1

Names

NAD-dependent protein deacetylase sirtuin-1
SIR2-like protein 1
regulatory protein SIR2 homolog 1
sirtuin type 1
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

10

10q21.3
Summary This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008].
Disorder MIM:

604479


Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_012238.5 NP_036370.2 NAD-dependent protein deacetylase sirtuin-1 isoform a
NM_001142498.1 NP_001135970.1 NAD-dependent protein deacetylase sirtuin-1 isoform b
NM_001314049.1 NP_001300978.1 NAD-dependent protein deacetylase sirtuin-1 isoform c
NM_012238.4 NP_036370.2 NAD-dependent protein deacetylase sirtuin-1 isoform a

Pathways from BioSystems


Homologies


Pan troglodytes (chimpanzee) SIRT1 XP_003312628.1
Rattus norvegicus (Norway rat) Sirt1 XP_006256208.1
Xenopus tropicalis (tropical clawed frog) sirt1 NP_001136381.1
Homo sapiens (human) SIRT1 NP_036370.2
Canis lupus familiaris (dog) SIRT1 XP_546130.2
Gallus gallus (chicken) SIRT1 NP_001004767.1
SIRT1 XP_001087854.2
Danio rerio (zebrafish) sirt1 XP_005173091.1
Bos taurus (cattle) SIRT1 NP_001179909.1
Mus musculus (house mouse) Sirt1 NP_062786.1

Gene Ontology

The following SIRT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SIRT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu26761 NM_001142498.1
Latest version!		 Homo sapiens sirtuin 1 (SIRT1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $189.50-$265.30
$379.00
OHu27002 NM_012238.5
Latest version!		 Homo sapiens sirtuin 1 (SIRT1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $279.50-$391.30
$559.00
OHu44172 NM_001314049.1
Latest version!		 Homo sapiens sirtuin 1 (SIRT1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
View Old Accession Versions >>
OHu27002 NM_012238.4 Homo sapiens sirtuin 1 (SIRT1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $279.50-$391.30
$559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu26761
Clone ID Related Accession (Same CDS sequence) NM_001142498.1
Accession Version NM_001142498.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1359bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-31
Organism Homo sapiens(human)
Product NAD-dependent protein deacetylase sirtuin-1 isoform b
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK289743.1, DB162635.1, AA452304.1 and BC012499.1. Transcript Variant: This variant (2) contains an alternate in-frame exon in the 5' coding region and uses a downstream start codon, compared to variant 1. Isoform b has a shorter N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AL136741.1, SRR1660803.163777.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142670 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGTTTGATA TTGAATATTT CAGAAAAGAT CCAAGACCAT TCTTCAAGTT TGCAAAGGAA 
ATATATCCTG GACAATTCCA GCCATCTCTC TGTCACAAAT TCATAGCCTT GTCAGATAAG 
GAAGGAAAAC TACTTCGCAA CTATACCCAG AACATAGACA CGCTGGAACA GGTTGCGGGA 
ATCCAAAGGA TAATTCAGTG TCATGGTTCC TTTGCAACAG CATCTTGCCT GATTTGTAAA 
TACAAAGTTG ACTGTGAAGC TGTACGAGGA GATATTTTTA ATCAGGTAGT TCCTCGATGT 
CCTAGGTGCC CAGCTGATGA ACCGCTTGCT ATCATGAAAC CAGAGATTGT GTTTTTTGGT 
GAAAATTTAC CAGAACAGTT TCATAGAGCC ATGAAGTATG ACAAAGATGA AGTTGACCTC 
CTCATTGTTA TTGGGTCTTC CCTCAAAGTA AGACCAGTAG CACTAATTCC AAGTTCCATA 
CCCCATGAAG TGCCTCAGAT ATTAATTAAT AGAGAACCTT TGCCTCATCT GCATTTTGAT 
GTAGAGCTTC TTGGAGACTG TGATGTCATA ATTAATGAAT TGTGTCATAG GTTAGGTGGT 
GAATATGCCA AACTTTGCTG TAACCCTGTA AAGCTTTCAG AAATTACTGA AAAACCTCCA 
CGAACACAAA AAGAATTGGC TTATTTGTCA GAGTTGCCAC CCACACCTCT TCATGTTTCA 
GAAGACTCAA GTTCACCAGA AAGAACTTCA CCACCAGATT CTTCAGTGAT TGTCACACTT 
TTAGACCAAG CAGCTAAGAG TAATGATGAT TTAGATGTGT CTGAATCAAA AGGTTGTATG 
GAAGAAAAAC CACAGGAAGT ACAAACTTCT AGGAATGTTG AAAGTATTGC TGAACAGATG 
GAAAATCCGG ATTTGAAGAA TGTTGGTTCT AGTACTGGGG AGAAAAATGA AAGAACTTCA 
GTGGCTGGAA CAGTGAGAAA ATGCTGGCCT AATAGAGTGG CAAAGGAGCA GATTAGTAGG 
CGGCTTGATG GTAATCAGTA TCTGTTTTTG CCACCAAATC GTTACATTTT CCATGGCGCT 
GAGGTATATT CAGACTCTGA AGATGACGTC TTATCCTCTA GTTCTTGTGG CAGTAACAGT 
GATAGTGGGA CATGCCAGAG TCCAAGTTTA GAAGAACCCA TGGAGGATGA AAGTGAAATT 
GAAGAATTCT ACAATGGCTT AGAAGATGAG CCTGATGTTC CAGAGAGAGC TGGAGGAGCT 
GGATTTGGGA CTGATGGAGA TGATCAAGAG GCAATTAATG AAGCTATATC TGTGAAACAG 
GAAGTAACAG ACATGAACTA TCCATCAAAC AAATCATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001135970.1
CDS433..1791
Translation

Target ORF information:

RefSeq Version NM_001142498.1
Organism Homo sapiens(human)
Definition Homo sapiens sirtuin 1 (SIRT1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001142498.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGTTTGATA TTGAATATTT CAGAAAAGAT CCAAGACCAT TCTTCAAGTT TGCAAAGGAA 
ATATATCCTG GACAATTCCA GCCATCTCTC TGTCACAAAT TCATAGCCTT GTCAGATAAG 
GAAGGAAAAC TACTTCGCAA CTATACCCAG AACATAGACA CGCTGGAACA GGTTGCGGGA 
ATCCAAAGGA TAATTCAGTG TCATGGTTCC TTTGCAACAG CATCTTGCCT GATTTGTAAA 
TACAAAGTTG ACTGTGAAGC TGTACGAGGA GATATTTTTA ATCAGGTAGT TCCTCGATGT 
CCTAGGTGCC CAGCTGATGA ACCGCTTGCT ATCATGAAAC CAGAGATTGT GTTTTTTGGT 
GAAAATTTAC CAGAACAGTT TCATAGAGCC ATGAAGTATG ACAAAGATGA AGTTGACCTC 
CTCATTGTTA TTGGGTCTTC CCTCAAAGTA AGACCAGTAG CACTAATTCC AAGTTCCATA 
CCCCATGAAG TGCCTCAGAT ATTAATTAAT AGAGAACCTT TGCCTCATCT GCATTTTGAT 
GTAGAGCTTC TTGGAGACTG TGATGTCATA ATTAATGAAT TGTGTCATAG GTTAGGTGGT 
GAATATGCCA AACTTTGCTG TAACCCTGTA AAGCTTTCAG AAATTACTGA AAAACCTCCA 
CGAACACAAA AAGAATTGGC TTATTTGTCA GAGTTGCCAC CCACACCTCT TCATGTTTCA 
GAAGACTCAA GTTCACCAGA AAGAACTTCA CCACCAGATT CTTCAGTGAT TGTCACACTT 
TTAGACCAAG CAGCTAAGAG TAATGATGAT TTAGATGTGT CTGAATCAAA AGGTTGTATG 
GAAGAAAAAC CACAGGAAGT ACAAACTTCT AGGAATGTTG AAAGTATTGC TGAACAGATG 
GAAAATCCGG ATTTGAAGAA TGTTGGTTCT AGTACTGGGG AGAAAAATGA AAGAACTTCA 
GTGGCTGGAA CAGTGAGAAA ATGCTGGCCT AATAGAGTGG CAAAGGAGCA GATTAGTAGG 
CGGCTTGATG GTAATCAGTA TCTGTTTTTG CCACCAAATC GTTACATTTT CCATGGCGCT 
GAGGTATATT CAGACTCTGA AGATGACGTC TTATCCTCTA GTTCTTGTGG CAGTAACAGT 
GATAGTGGGA CATGCCAGAG TCCAAGTTTA GAAGAACCCA TGGAGGATGA AAGTGAAATT 
GAAGAATTCT ACAATGGCTT AGAAGATGAG CCTGATGTTC CAGAGAGAGC TGGAGGAGCT 
GGATTTGGGA CTGATGGAGA TGATCAAGAG GCAATTAATG AAGCTATATC TGTGAAACAG 
GAAGTAACAG ACATGAACTA TCCATCAAAC AAATCATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27002
Clone ID Related Accession (Same CDS sequence) NM_012238.5 , NM_012238.4
Accession Version NM_012238.5 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2244bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2020-01-01
Organism Homo sapiens(human)
Product NAD-dependent protein deacetylase sirtuin-1 isoform a
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL133551.13. On Nov 23, 2018 this sequence version replaced NM_012238.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC012499.1, AF083106.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000212015.11/ ENSP00000212015.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGCGGACG AGGCGGCCCT CGCCCTTCAG CCCGGCGGCT CCCCCTCGGC GGCGGGGGCC 
GACAGGGAGG CCGCGTCGTC CCCCGCCGGG GAGCCGCTCC GCAAGAGGCC GCGGAGAGAT 
GGTCCCGGCC TCGAGCGGAG CCCGGGCGAG CCCGGTGGGG CGGCCCCAGA GCGTGAGGTG 
CCGGCGGCGG CCAGGGGCTG CCCGGGTGCG GCGGCGGCGG CGCTGTGGCG GGAGGCGGAG 
GCAGAGGCGG CGGCGGCAGG CGGGGAGCAA GAGGCCCAGG CGACTGCGGC GGCTGGGGAA 
GGAGACAATG GGCCGGGCCT GCAGGGCCCA TCTCGGGAGC CACCGCTGGC CGACAACTTG 
TACGACGAAG ACGACGACGA CGAGGGCGAG GAGGAGGAAG AGGCGGCGGC GGCGGCGATT 
GGGTACCGAG ATAACCTTCT GTTCGGTGAT GAAATTATCA CTAATGGTTT TCATTCCTGT 
GAAAGTGATG AGGAGGATAG AGCCTCACAT GCAAGCTCTA GTGACTGGAC TCCAAGGCCA 
CGGATAGGTC CATATACTTT TGTTCAGCAA CATCTTATGA TTGGCACAGA TCCTCGAACA 
ATTCTTAAAG ATTTATTGCC GGAAACAATA CCTCCACCTG AGTTGGATGA TATGACACTG 
TGGCAGATTG TTATTAATAT CCTTTCAGAA CCACCAAAAA GGAAAAAAAG AAAAGATATT 
AATACAATTG AAGATGCTGT GAAATTACTG CAAGAGTGCA AAAAAATTAT AGTTCTAACT 
GGAGCTGGGG TGTCTGTTTC ATGTGGAATA CCTGACTTCA GGTCAAGGGA TGGTATTTAT 
GCTCGCCTTG CTGTAGACTT CCCAGATCTT CCAGATCCTC AAGCGATGTT TGATATTGAA 
TATTTCAGAA AAGATCCAAG ACCATTCTTC AAGTTTGCAA AGGAAATATA TCCTGGACAA 
TTCCAGCCAT CTCTCTGTCA CAAATTCATA GCCTTGTCAG ATAAGGAAGG AAAACTACTT 
CGCAACTATA CCCAGAACAT AGACACGCTG GAACAGGTTG CGGGAATCCA AAGGATAATT 
CAGTGTCATG GTTCCTTTGC AACAGCATCT TGCCTGATTT GTAAATACAA AGTTGACTGT 
GAAGCTGTAC GAGGAGATAT TTTTAATCAG GTAGTTCCTC GATGTCCTAG GTGCCCAGCT 
GATGAACCGC TTGCTATCAT GAAACCAGAG ATTGTGTTTT TTGGTGAAAA TTTACCAGAA 
CAGTTTCATA GAGCCATGAA GTATGACAAA GATGAAGTTG ACCTCCTCAT TGTTATTGGG 
TCTTCCCTCA AAGTAAGACC AGTAGCACTA ATTCCAAGTT CCATACCCCA TGAAGTGCCT 
CAGATATTAA TTAATAGAGA ACCTTTGCCT CATCTGCATT TTGATGTAGA GCTTCTTGGA 
GACTGTGATG TCATAATTAA TGAATTGTGT CATAGGTTAG GTGGTGAATA TGCCAAACTT 
TGCTGTAACC CTGTAAAGCT TTCAGAAATT ACTGAAAAAC CTCCACGAAC ACAAAAAGAA 
TTGGCTTATT TGTCAGAGTT GCCACCCACA CCTCTTCATG TTTCAGAAGA CTCAAGTTCA 
CCAGAAAGAA CTTCACCACC AGATTCTTCA GTGATTGTCA CACTTTTAGA CCAAGCAGCT 
AAGAGTAATG ATGATTTAGA TGTGTCTGAA TCAAAAGGTT GTATGGAAGA AAAACCACAG 
GAAGTACAAA CTTCTAGGAA TGTTGAAAGT ATTGCTGAAC AGATGGAAAA TCCGGATTTG 
AAGAATGTTG GTTCTAGTAC TGGGGAGAAA AATGAAAGAA CTTCAGTGGC TGGAACAGTG 
AGAAAATGCT GGCCTAATAG AGTGGCAAAG GAGCAGATTA GTAGGCGGCT TGATGGTAAT 
CAGTATCTGT TTTTGCCACC AAATCGTTAC ATTTTCCATG GCGCTGAGGT ATATTCAGAC 
TCTGAAGATG ACGTCTTATC CTCTAGTTCT TGTGGCAGTA ACAGTGATAG TGGGACATGC 
CAGAGTCCAA GTTTAGAAGA ACCCATGGAG GATGAAAGTG AAATTGAAGA ATTCTACAAT 
GGCTTAGAAG ATGAGCCTGA TGTTCCAGAG AGAGCTGGAG GAGCTGGATT TGGGACTGAT 
GGAGATGATC AAGAGGCAAT TAATGAAGCT ATATCTGTGA AACAGGAAGT AACAGACATG 
AACTATCCAT CAAACAAATC ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_036370.2
CDS67..2310
Translation

Target ORF information:

RefSeq Version NM_012238.5
Organism Homo sapiens(human)
Definition Homo sapiens sirtuin 1 (SIRT1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_012238.5

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGCGGACG AGGCGGCCCT CGCCCTTCAG CCCGGCGGCT CCCCCTCGGC GGCGGGGGCC 
GACAGGGAGG CCGCGTCGTC CCCCGCCGGG GAGCCGCTCC GCAAGAGGCC GCGGAGAGAT 
GGTCCCGGCC TCGAGCGGAG CCCGGGCGAG CCCGGTGGGG CGGCCCCAGA GCGTGAGGTG 
CCGGCGGCGG CCAGGGGCTG CCCGGGTGCG GCGGCGGCGG CGCTGTGGCG GGAGGCGGAG 
GCAGAGGCGG CGGCGGCAGG CGGGGAGCAA GAGGCCCAGG CGACTGCGGC GGCTGGGGAA 
GGAGACAATG GGCCGGGCCT GCAGGGCCCA TCTCGGGAGC CACCGCTGGC CGACAACTTG 
TACGACGAAG ACGACGACGA CGAGGGCGAG GAGGAGGAAG AGGCGGCGGC GGCGGCGATT 
GGGTACCGAG ATAACCTTCT GTTCGGTGAT GAAATTATCA CTAATGGTTT TCATTCCTGT 
GAAAGTGATG AGGAGGATAG AGCCTCACAT GCAAGCTCTA GTGACTGGAC TCCAAGGCCA 
CGGATAGGTC CATATACTTT TGTTCAGCAA CATCTTATGA TTGGCACAGA TCCTCGAACA 
ATTCTTAAAG ATTTATTGCC GGAAACAATA CCTCCACCTG AGTTGGATGA TATGACACTG 
TGGCAGATTG TTATTAATAT CCTTTCAGAA CCACCAAAAA GGAAAAAAAG AAAAGATATT 
AATACAATTG AAGATGCTGT GAAATTACTG CAAGAGTGCA AAAAAATTAT AGTTCTAACT 
GGAGCTGGGG TGTCTGTTTC ATGTGGAATA CCTGACTTCA GGTCAAGGGA TGGTATTTAT 
GCTCGCCTTG CTGTAGACTT CCCAGATCTT CCAGATCCTC AAGCGATGTT TGATATTGAA 
TATTTCAGAA AAGATCCAAG ACCATTCTTC AAGTTTGCAA AGGAAATATA TCCTGGACAA 
TTCCAGCCAT CTCTCTGTCA CAAATTCATA GCCTTGTCAG ATAAGGAAGG AAAACTACTT 
CGCAACTATA CCCAGAACAT AGACACGCTG GAACAGGTTG CGGGAATCCA AAGGATAATT 
CAGTGTCATG GTTCCTTTGC AACAGCATCT TGCCTGATTT GTAAATACAA AGTTGACTGT 
GAAGCTGTAC GAGGAGATAT TTTTAATCAG GTAGTTCCTC GATGTCCTAG GTGCCCAGCT 
GATGAACCGC TTGCTATCAT GAAACCAGAG ATTGTGTTTT TTGGTGAAAA TTTACCAGAA 
CAGTTTCATA GAGCCATGAA GTATGACAAA GATGAAGTTG ACCTCCTCAT TGTTATTGGG 
TCTTCCCTCA AAGTAAGACC AGTAGCACTA ATTCCAAGTT CCATACCCCA TGAAGTGCCT 
CAGATATTAA TTAATAGAGA ACCTTTGCCT CATCTGCATT TTGATGTAGA GCTTCTTGGA 
GACTGTGATG TCATAATTAA TGAATTGTGT CATAGGTTAG GTGGTGAATA TGCCAAACTT 
TGCTGTAACC CTGTAAAGCT TTCAGAAATT ACTGAAAAAC CTCCACGAAC ACAAAAAGAA 
TTGGCTTATT TGTCAGAGTT GCCACCCACA CCTCTTCATG TTTCAGAAGA CTCAAGTTCA 
CCAGAAAGAA CTTCACCACC AGATTCTTCA GTGATTGTCA CACTTTTAGA CCAAGCAGCT 
AAGAGTAATG ATGATTTAGA TGTGTCTGAA TCAAAAGGTT GTATGGAAGA AAAACCACAG 
GAAGTACAAA CTTCTAGGAA TGTTGAAAGT ATTGCTGAAC AGATGGAAAA TCCGGATTTG 
AAGAATGTTG GTTCTAGTAC TGGGGAGAAA AATGAAAGAA CTTCAGTGGC TGGAACAGTG 
AGAAAATGCT GGCCTAATAG AGTGGCAAAG GAGCAGATTA GTAGGCGGCT TGATGGTAAT 
CAGTATCTGT TTTTGCCACC AAATCGTTAC ATTTTCCATG GCGCTGAGGT ATATTCAGAC 
TCTGAAGATG ACGTCTTATC CTCTAGTTCT TGTGGCAGTA ACAGTGATAG TGGGACATGC 
CAGAGTCCAA GTTTAGAAGA ACCCATGGAG GATGAAAGTG AAATTGAAGA ATTCTACAAT 
GGCTTAGAAG ATGAGCCTGA TGTTCCAGAG AGAGCTGGAG GAGCTGGATT TGGGACTGAT 
GGAGATGATC AAGAGGCAAT TAATGAAGCT ATATCTGTGA AACAGGAAGT AACAGACATG 
AACTATCCAT CAAACAAATC ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27002
Clone ID Related Accession (Same CDS sequence) NM_012238.5 , NM_012238.4
Accession Version NM_012238.4 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2244bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-12-05
Organism Homo sapiens(human)
Product NAD-dependent protein deacetylase sirtuin-1 isoform a
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF083106.2, AA452304.1 and BC012499.1. This sequence is a reference standard in the RefSeqGene project. On Dec 5, 2008 this sequence version replaced NM_012238.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF083106.2, AL136741.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGCGGACG AGGCGGCCCT CGCCCTTCAG CCCGGCGGCT CCCCCTCGGC GGCGGGGGCC 
GACAGGGAGG CCGCGTCGTC CCCCGCCGGG GAGCCGCTCC GCAAGAGGCC GCGGAGAGAT 
GGTCCCGGCC TCGAGCGGAG CCCGGGCGAG CCCGGTGGGG CGGCCCCAGA GCGTGAGGTG 
CCGGCGGCGG CCAGGGGCTG CCCGGGTGCG GCGGCGGCGG CGCTGTGGCG GGAGGCGGAG 
GCAGAGGCGG CGGCGGCAGG CGGGGAGCAA GAGGCCCAGG CGACTGCGGC GGCTGGGGAA 
GGAGACAATG GGCCGGGCCT GCAGGGCCCA TCTCGGGAGC CACCGCTGGC CGACAACTTG 
TACGACGAAG ACGACGACGA CGAGGGCGAG GAGGAGGAAG AGGCGGCGGC GGCGGCGATT 
GGGTACCGAG ATAACCTTCT GTTCGGTGAT GAAATTATCA CTAATGGTTT TCATTCCTGT 
GAAAGTGATG AGGAGGATAG AGCCTCACAT GCAAGCTCTA GTGACTGGAC TCCAAGGCCA 
CGGATAGGTC CATATACTTT TGTTCAGCAA CATCTTATGA TTGGCACAGA TCCTCGAACA 
ATTCTTAAAG ATTTATTGCC GGAAACAATA CCTCCACCTG AGTTGGATGA TATGACACTG 
TGGCAGATTG TTATTAATAT CCTTTCAGAA CCACCAAAAA GGAAAAAAAG AAAAGATATT 
AATACAATTG AAGATGCTGT GAAATTACTG CAAGAGTGCA AAAAAATTAT AGTTCTAACT 
GGAGCTGGGG TGTCTGTTTC ATGTGGAATA CCTGACTTCA GGTCAAGGGA TGGTATTTAT 
GCTCGCCTTG CTGTAGACTT CCCAGATCTT CCAGATCCTC AAGCGATGTT TGATATTGAA 
TATTTCAGAA AAGATCCAAG ACCATTCTTC AAGTTTGCAA AGGAAATATA TCCTGGACAA 
TTCCAGCCAT CTCTCTGTCA CAAATTCATA GCCTTGTCAG ATAAGGAAGG AAAACTACTT 
CGCAACTATA CCCAGAACAT AGACACGCTG GAACAGGTTG CGGGAATCCA AAGGATAATT 
CAGTGTCATG GTTCCTTTGC AACAGCATCT TGCCTGATTT GTAAATACAA AGTTGACTGT 
GAAGCTGTAC GAGGAGATAT TTTTAATCAG GTAGTTCCTC GATGTCCTAG GTGCCCAGCT 
GATGAACCGC TTGCTATCAT GAAACCAGAG ATTGTGTTTT TTGGTGAAAA TTTACCAGAA 
CAGTTTCATA GAGCCATGAA GTATGACAAA GATGAAGTTG ACCTCCTCAT TGTTATTGGG 
TCTTCCCTCA AAGTAAGACC AGTAGCACTA ATTCCAAGTT CCATACCCCA TGAAGTGCCT 
CAGATATTAA TTAATAGAGA ACCTTTGCCT CATCTGCATT TTGATGTAGA GCTTCTTGGA 
GACTGTGATG TCATAATTAA TGAATTGTGT CATAGGTTAG GTGGTGAATA TGCCAAACTT 
TGCTGTAACC CTGTAAAGCT TTCAGAAATT ACTGAAAAAC CTCCACGAAC ACAAAAAGAA 
TTGGCTTATT TGTCAGAGTT GCCACCCACA CCTCTTCATG TTTCAGAAGA CTCAAGTTCA 
CCAGAAAGAA CTTCACCACC AGATTCTTCA GTGATTGTCA CACTTTTAGA CCAAGCAGCT 
AAGAGTAATG ATGATTTAGA TGTGTCTGAA TCAAAAGGTT GTATGGAAGA AAAACCACAG 
GAAGTACAAA CTTCTAGGAA TGTTGAAAGT ATTGCTGAAC AGATGGAAAA TCCGGATTTG 
AAGAATGTTG GTTCTAGTAC TGGGGAGAAA AATGAAAGAA CTTCAGTGGC TGGAACAGTG 
AGAAAATGCT GGCCTAATAG AGTGGCAAAG GAGCAGATTA GTAGGCGGCT TGATGGTAAT 
CAGTATCTGT TTTTGCCACC AAATCGTTAC ATTTTCCATG GCGCTGAGGT ATATTCAGAC 
TCTGAAGATG ACGTCTTATC CTCTAGTTCT TGTGGCAGTA ACAGTGATAG TGGGACATGC 
CAGAGTCCAA GTTTAGAAGA ACCCATGGAG GATGAAAGTG AAATTGAAGA ATTCTACAAT 
GGCTTAGAAG ATGAGCCTGA TGTTCCAGAG AGAGCTGGAG GAGCTGGATT TGGGACTGAT 
GGAGATGATC AAGAGGCAAT TAATGAAGCT ATATCTGTGA AACAGGAAGT AACAGACATG 
AACTATCCAT CAAACAAATC ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_036370.2
CDS54..2297
Translation

Target ORF information:

RefSeq Version NM_012238.4
Organism Homo sapiens(human)
Definition Homo sapiens sirtuin 1 (SIRT1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_012238.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGCGGACG AGGCGGCCCT CGCCCTTCAG CCCGGCGGCT CCCCCTCGGC GGCGGGGGCC 
GACAGGGAGG CCGCGTCGTC CCCCGCCGGG GAGCCGCTCC GCAAGAGGCC GCGGAGAGAT 
GGTCCCGGCC TCGAGCGGAG CCCGGGCGAG CCCGGTGGGG CGGCCCCAGA GCGTGAGGTG 
CCGGCGGCGG CCAGGGGCTG CCCGGGTGCG GCGGCGGCGG CGCTGTGGCG GGAGGCGGAG 
GCAGAGGCGG CGGCGGCAGG CGGGGAGCAA GAGGCCCAGG CGACTGCGGC GGCTGGGGAA 
GGAGACAATG GGCCGGGCCT GCAGGGCCCA TCTCGGGAGC CACCGCTGGC CGACAACTTG 
TACGACGAAG ACGACGACGA CGAGGGCGAG GAGGAGGAAG AGGCGGCGGC GGCGGCGATT 
GGGTACCGAG ATAACCTTCT GTTCGGTGAT GAAATTATCA CTAATGGTTT TCATTCCTGT 
GAAAGTGATG AGGAGGATAG AGCCTCACAT GCAAGCTCTA GTGACTGGAC TCCAAGGCCA 
CGGATAGGTC CATATACTTT TGTTCAGCAA CATCTTATGA TTGGCACAGA TCCTCGAACA 
ATTCTTAAAG ATTTATTGCC GGAAACAATA CCTCCACCTG AGTTGGATGA TATGACACTG 
TGGCAGATTG TTATTAATAT CCTTTCAGAA CCACCAAAAA GGAAAAAAAG AAAAGATATT 
AATACAATTG AAGATGCTGT GAAATTACTG CAAGAGTGCA AAAAAATTAT AGTTCTAACT 
GGAGCTGGGG TGTCTGTTTC ATGTGGAATA CCTGACTTCA GGTCAAGGGA TGGTATTTAT 
GCTCGCCTTG CTGTAGACTT CCCAGATCTT CCAGATCCTC AAGCGATGTT TGATATTGAA 
TATTTCAGAA AAGATCCAAG ACCATTCTTC AAGTTTGCAA AGGAAATATA TCCTGGACAA 
TTCCAGCCAT CTCTCTGTCA CAAATTCATA GCCTTGTCAG ATAAGGAAGG AAAACTACTT 
CGCAACTATA CCCAGAACAT AGACACGCTG GAACAGGTTG CGGGAATCCA AAGGATAATT 
CAGTGTCATG GTTCCTTTGC AACAGCATCT TGCCTGATTT GTAAATACAA AGTTGACTGT 
GAAGCTGTAC GAGGAGATAT TTTTAATCAG GTAGTTCCTC GATGTCCTAG GTGCCCAGCT 
GATGAACCGC TTGCTATCAT GAAACCAGAG ATTGTGTTTT TTGGTGAAAA TTTACCAGAA 
CAGTTTCATA GAGCCATGAA GTATGACAAA GATGAAGTTG ACCTCCTCAT TGTTATTGGG 
TCTTCCCTCA AAGTAAGACC AGTAGCACTA ATTCCAAGTT CCATACCCCA TGAAGTGCCT 
CAGATATTAA TTAATAGAGA ACCTTTGCCT CATCTGCATT TTGATGTAGA GCTTCTTGGA 
GACTGTGATG TCATAATTAA TGAATTGTGT CATAGGTTAG GTGGTGAATA TGCCAAACTT 
TGCTGTAACC CTGTAAAGCT TTCAGAAATT ACTGAAAAAC CTCCACGAAC ACAAAAAGAA 
TTGGCTTATT TGTCAGAGTT GCCACCCACA CCTCTTCATG TTTCAGAAGA CTCAAGTTCA 
CCAGAAAGAA CTTCACCACC AGATTCTTCA GTGATTGTCA CACTTTTAGA CCAAGCAGCT 
AAGAGTAATG ATGATTTAGA TGTGTCTGAA TCAAAAGGTT GTATGGAAGA AAAACCACAG 
GAAGTACAAA CTTCTAGGAA TGTTGAAAGT ATTGCTGAAC AGATGGAAAA TCCGGATTTG 
AAGAATGTTG GTTCTAGTAC TGGGGAGAAA AATGAAAGAA CTTCAGTGGC TGGAACAGTG 
AGAAAATGCT GGCCTAATAG AGTGGCAAAG GAGCAGATTA GTAGGCGGCT TGATGGTAAT 
CAGTATCTGT TTTTGCCACC AAATCGTTAC ATTTTCCATG GCGCTGAGGT ATATTCAGAC 
TCTGAAGATG ACGTCTTATC CTCTAGTTCT TGTGGCAGTA ACAGTGATAG TGGGACATGC 
CAGAGTCCAA GTTTAGAAGA ACCCATGGAG GATGAAAGTG AAATTGAAGA ATTCTACAAT 
GGCTTAGAAG ATGAGCCTGA TGTTCCAGAG AGAGCTGGAG GAGCTGGATT TGGGACTGAT 
GGAGATGATC AAGAGGCAAT TAATGAAGCT ATATCTGTGA AACAGGAAGT AACAGACATG 
AACTATCCAT CAAACAAATC ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu44172
Clone ID Related Accession (Same CDS sequence) NM_001314049.1
Accession Version NM_001314049.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1335bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2020-01-01
Organism Homo sapiens(human)
Product NAD-dependent protein deacetylase sirtuin-1 isoform c
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB162635.1, AK074805.1, AF235040.1 and BC012499.1. On Sep 29, 2015 this sequence version replaced XM_005269663.1. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region and uses an alternate translation start site compared to variant 1. The encoded isoform (c) is shorter and has a distinct N-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK074805.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGTGCCTGT GCAGTGGAAG GAAAACAATT TTGGAAATAT ATCCTGGACA ATTCCAGCCA 
TCTCTCTGTC ACAAATTCAT AGCCTTGTCA GATAAGGAAG GAAAACTACT TCGCAACTAT 
ACCCAGAACA TAGACACGCT GGAACAGGTT GCGGGAATCC AAAGGATAAT TCAGTGTCAT 
GGTTCCTTTG CAACAGCATC TTGCCTGATT TGTAAATACA AAGTTGACTG TGAAGCTGTA 
CGAGGAGATA TTTTTAATCA GGTAGTTCCT CGATGTCCTA GGTGCCCAGC TGATGAACCG 
CTTGCTATCA TGAAACCAGA GATTGTGTTT TTTGGTGAAA ATTTACCAGA ACAGTTTCAT 
AGAGCCATGA AGTATGACAA AGATGAAGTT GACCTCCTCA TTGTTATTGG GTCTTCCCTC 
AAAGTAAGAC CAGTAGCACT AATTCCAAGT TCCATACCCC ATGAAGTGCC TCAGATATTA 
ATTAATAGAG AACCTTTGCC TCATCTGCAT TTTGATGTAG AGCTTCTTGG AGACTGTGAT 
GTCATAATTA ATGAATTGTG TCATAGGTTA GGTGGTGAAT ATGCCAAACT TTGCTGTAAC 
CCTGTAAAGC TTTCAGAAAT TACTGAAAAA CCTCCACGAA CACAAAAAGA ATTGGCTTAT 
TTGTCAGAGT TGCCACCCAC ACCTCTTCAT GTTTCAGAAG ACTCAAGTTC ACCAGAAAGA 
ACTTCACCAC CAGATTCTTC AGTGATTGTC ACACTTTTAG ACCAAGCAGC TAAGAGTAAT 
GATGATTTAG ATGTGTCTGA ATCAAAAGGT TGTATGGAAG AAAAACCACA GGAAGTACAA 
ACTTCTAGGA ATGTTGAAAG TATTGCTGAA CAGATGGAAA ATCCGGATTT GAAGAATGTT 
GGTTCTAGTA CTGGGGAGAA AAATGAAAGA ACTTCAGTGG CTGGAACAGT GAGAAAATGC 
TGGCCTAATA GAGTGGCAAA GGAGCAGATT AGTAGGCGGC TTGATGGTAA TCAGTATCTG 
TTTTTGCCAC CAAATCGTTA CATTTTCCAT GGCGCTGAGG TATATTCAGA CTCTGAAGAT 
GACGTCTTAT CCTCTAGTTC TTGTGGCAGT AACAGTGATA GTGGGACATG CCAGAGTCCA 
AGTTTAGAAG AACCCATGGA GGATGAAAGT GAAATTGAAG AATTCTACAA TGGCTTAGAA 
GATGAGCCTG ATGTTCCAGA GAGAGCTGGA GGAGCTGGAT TTGGGACTGA TGGAGATGAT 
CAAGAGGCAA TTAATGAAGC TATATCTGTG AAACAGGAAG TAACAGACAT GAACTATCCA 
TCAAACAAAT CATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001300978.1
CDS246..1580
Translation

Target ORF information:

RefSeq Version NM_001314049.1
Organism Homo sapiens(human)
Definition Homo sapiens sirtuin 1 (SIRT1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001314049.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGTGCCTGT GCAGTGGAAG GAAAACAATT TTGGAAATAT ATCCTGGACA ATTCCAGCCA 
TCTCTCTGTC ACAAATTCAT AGCCTTGTCA GATAAGGAAG GAAAACTACT TCGCAACTAT 
ACCCAGAACA TAGACACGCT GGAACAGGTT GCGGGAATCC AAAGGATAAT TCAGTGTCAT 
GGTTCCTTTG CAACAGCATC TTGCCTGATT TGTAAATACA AAGTTGACTG TGAAGCTGTA 
CGAGGAGATA TTTTTAATCA GGTAGTTCCT CGATGTCCTA GGTGCCCAGC TGATGAACCG 
CTTGCTATCA TGAAACCAGA GATTGTGTTT TTTGGTGAAA ATTTACCAGA ACAGTTTCAT 
AGAGCCATGA AGTATGACAA AGATGAAGTT GACCTCCTCA TTGTTATTGG GTCTTCCCTC 
AAAGTAAGAC CAGTAGCACT AATTCCAAGT TCCATACCCC ATGAAGTGCC TCAGATATTA 
ATTAATAGAG AACCTTTGCC TCATCTGCAT TTTGATGTAG AGCTTCTTGG AGACTGTGAT 
GTCATAATTA ATGAATTGTG TCATAGGTTA GGTGGTGAAT ATGCCAAACT TTGCTGTAAC 
CCTGTAAAGC TTTCAGAAAT TACTGAAAAA CCTCCACGAA CACAAAAAGA ATTGGCTTAT 
TTGTCAGAGT TGCCACCCAC ACCTCTTCAT GTTTCAGAAG ACTCAAGTTC ACCAGAAAGA 
ACTTCACCAC CAGATTCTTC AGTGATTGTC ACACTTTTAG ACCAAGCAGC TAAGAGTAAT 
GATGATTTAG ATGTGTCTGA ATCAAAAGGT TGTATGGAAG AAAAACCACA GGAAGTACAA 
ACTTCTAGGA ATGTTGAAAG TATTGCTGAA CAGATGGAAA ATCCGGATTT GAAGAATGTT 
GGTTCTAGTA CTGGGGAGAA AAATGAAAGA ACTTCAGTGG CTGGAACAGT GAGAAAATGC 
TGGCCTAATA GAGTGGCAAA GGAGCAGATT AGTAGGCGGC TTGATGGTAA TCAGTATCTG 
TTTTTGCCAC CAAATCGTTA CATTTTCCAT GGCGCTGAGG TATATTCAGA CTCTGAAGAT 
GACGTCTTAT CCTCTAGTTC TTGTGGCAGT AACAGTGATA GTGGGACATG CCAGAGTCCA 
AGTTTAGAAG AACCCATGGA GGATGAAAGT GAAATTGAAG AATTCTACAA TGGCTTAGAA 
GATGAGCCTG ATGTTCCAGA GAGAGCTGGA GGAGCTGGAT TTGGGACTGA TGGAGATGAT 
CAAGAGGCAA TTAATGAAGC TATATCTGTG AAACAGGAAG TAACAGACAT GAACTATCCA 
TCAAACAAAT CATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed