×

GAPDH cDNA ORF clone, Homo sapiens (human)

Gene Symbol GAPDH
Entrez Gene ID 2597
Full Name glyceraldehyde-3-phosphate dehydrogenase
Synonyms G3PD, GAPD, HEL-S-162eP
General protein information
Preferred Names
glyceraldehyde-3-phosphate dehydrogenase
Names
glyceraldehyde-3-phosphate dehydrogenase
aging-associated gene 9 protein
peptidyl-cysteine S-nitrosylase GAPDH
NP_001243728.1
NP_002037.2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

12

12p13

Summary This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]. lac of sum
Disorder MIM:

138400


mRNA and Protein(s)

mRNA Protein Name
NM_002046 NP_002037 glyceraldehyde-3-phosphate dehydrogenase isoform 1
NM_001256799 NP_001243728 glyceraldehyde-3-phosphate dehydrogenase isoform 2
NM_001289746 NP_001276675 glyceraldehyde-3-phosphate dehydrogenase isoform 1
NM_001289745 NP_001276674 glyceraldehyde-3-phosphate dehydrogenase isoform 1



Homo sapiens (human) GAPDH NP_002037.2
Pan troglodytes (chimpanzee) GAPDH XP_508955.1
Macaca mulatta (Rhesus monkey) GAPDH XP_001105471.1
Canis lupus familiaris (dog) GAPDH NP_001003142.1
Canis lupus familiaris (dog) LOC100688969 XP_003435697.1
Canis lupus familiaris (dog) LOC100683724 XP_003434435.1
Bos taurus (cattle) GAPDH NP_001029206.1
Mus musculus (house mouse) Gapdh NP_032110.1
Mus musculus (house mouse) Gm20899 XP_001476757.1
Rattus norvegicus (Norway rat) Gapdh NP_058704.1
Gallus gallus (chicken) GAPDH NP_989636.1
Danio rerio (zebrafish) gapdh NP_001108586.1
Drosophila melanogaster (fruit fly) Gapdh2 NP_001259584.1
Drosophila melanogaster (fruit fly) Gapdh1 NP_525108.2
Caenorhabditis elegans gpd-1 NP_496237.1
Caenorhabditis elegans gpd-4 NP_496192.1
Caenorhabditis elegans gpd-3 NP_508534.3
Caenorhabditis elegans gpd-2 NP_508535.1
Xenopus (Silurana) tropicalis (western clawed frog) gapdh NP_001004949.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GAPDH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GAPDH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Free ORF Clone
for Every Scientist
More Detail >>

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu20566
NM_002046 Homo sapiens glyceraldehyde-3-phosphate dehydrogenase (GAPDH), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu20465
NM_001256799 Homo sapiens glyceraldehyde-3-phosphate dehydrogenase (GAPDH), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu20566
NM_001289746 Homo sapiens glyceraldehyde-3-phosphate dehydrogenase (GAPDH), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu20566
NM_001289745 Homo sapiens glyceraldehyde-3-phosphate dehydrogenase (GAPDH), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu20566
Clone ID Related Accession (Same CDS sequence) NM_001289745 , NM_001289746 , NM_002046
Accession Version NM_002046.5 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1008bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product glyceraldehyde-3-phosphate dehydrogenase isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from HY046784.1, BC009081.1 and BE893087.1. This sequence is a reference standard in the RefSeqGene project. On Jan 28, 2014 this sequence version replaced gi:378404906. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## multifunctional gene product(s) :: PMID: 20727968 Protein has antimicrobial activity :: PMID: 22832495 ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AF261085.1, BC013310.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGGGAAGG TGAAGGTCGG AGTCAACGGA TTTGGTCGTA TTGGGCGCCT GGTCACCAGG 
GCTGCTTTTA ACTCTGGTAA AGTGGATATT GTTGCCATCA ATGACCCCTT CATTGACCTC
AACTACATGG TTTACATGTT CCAATATGAT TCCACCCATG GCAAATTCCA TGGCACCGTC
AAGGCTGAGA ACGGGAAGCT TGTCATCAAT GGAAATCCCA TCACCATCTT CCAGGAGCGA
GATCCCTCCA AAATCAAGTG GGGCGATGCT GGCGCTGAGT ACGTCGTGGA GTCCACTGGC
GTCTTCACCA CCATGGAGAA GGCTGGGGCT CATTTGCAGG GGGGAGCCAA AAGGGTCATC
ATCTCTGCCC CCTCTGCTGA TGCCCCCATG TTCGTCATGG GTGTGAACCA TGAGAAGTAT
GACAACAGCC TCAAGATCAT CAGCAATGCC TCCTGCACCA CCAACTGCTT AGCACCCCTG
GCCAAGGTCA TCCATGACAA CTTTGGTATC GTGGAAGGAC TCATGACCAC AGTCCATGCC
ATCACTGCCA CCCAGAAGAC TGTGGATGGC CCCTCCGGGA AACTGTGGCG TGATGGCCGC
GGGGCTCTCC AGAACATCAT CCCTGCCTCT ACTGGCGCTG CCAAGGCTGT GGGCAAGGTC
ATCCCTGAGC TGAACGGGAA GCTCACTGGC ATGGCCTTCC GTGTCCCCAC TGCCAACGTG
TCAGTGGTGG ACCTGACCTG CCGTCTAGAA AAACCTGCCA AATATGATGA CATCAAGAAG
GTGGTGAAGC AGGCGTCGGA GGGCCCCCTC AAGGGCATCC TGGGCTACAC TGAGCACCAG
GTGGTCTCCT CTGACTTCAA CAGCGACACC CACTCCTCCA CCTTTGACGC TGGGGCTGGC
ATTGCCCTCA ACGACCACTT TGTCAAGCTC ATTTCCTGGT ATGACAACGA ATTTGGCTAC
AGCAACAGGG TGGTGGACCT CATGGCCCAC ATGGCCTCCA AGGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002037.2
CDS189..1196
Misc Feature(1)192..632(+)
Misc Feature(2)195..1193(+)
Misc Feature(3)198..644(+)
Misc Feature(4)201..203(+)
Misc Feature(5)213..215(+)
Misc Feature(6)312..314(+)
Misc Feature(7)312..314(+)
Misc Feature(8)369..371(+)
Misc Feature(9)378..380(+)
Misc Feature(10)384..386(+)
Misc Feature(11)396..398(+)
Misc Feature(12)411..413(+)
Misc Feature(13)435..437(+)
Misc Feature(14)435..437(+)
Misc Feature(15)552..554(+)
Misc Feature(16)630..632(+)
Misc Feature(17)633..635(+)
Misc Feature(18)639..647(+)
Misc Feature(19)639..641(+)
Misc Feature(20)642..656(+)
Misc Feature(21)642..644(+)
Misc Feature(22)642..644(+)
Misc Feature(23)642..644(+)
Misc Feature(24)642..644(+)
Misc Feature(25)651..653(+)
Misc Feature(26)657..1130(+)
Misc Feature(27)723..725(+)
Misc Feature(28)738..740(+)
Misc Feature(29)768..770(+)
Misc Feature(30)768..770(+)
Misc Feature(31)768..770(+)
Misc Feature(32)819..824(+)
Misc Feature(33)819..821(+)
Misc Feature(34)831..833(+)
Misc Feature(35)831..833(+)
Misc Feature(36)843..845(+)
Misc Feature(37)861..863(+)
Misc Feature(38)867..869(+)
Misc Feature(39)867..869(+)
Misc Feature(40)873..875(+)
Misc Feature(41)897..899(+)
Misc Feature(42)948..950(+)
Misc Feature(43)966..968(+)
Misc Feature(44)975..977(+)
Misc Feature(45)1122..1124(+)
Misc Feature(46)1134..1136(+)
Misc Feature(47)1188..1190(+)
Exon (1)1..165
Gene:GAPDH
Gene Synonym:
Exon (2)166..217
Gene:GAPDH
Gene Synonym:
Exon (3)218..317
Gene:GAPDH
Gene Synonym:
Exon (4)318..424
Gene:GAPDH
Gene Synonym:
Exon (5)425..515
Gene:GAPDH
Gene Synonym:
Exon (6)516..631
Gene:GAPDH
Gene Synonym:
Exon (7)632..713
Gene:GAPDH
Gene Synonym:
Exon (8)714..1126
Gene:GAPDH
Gene Synonym:
Exon (9)1127..1401
Gene:GAPDH
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_002046
Organism Homo sapiens (human)
Definition Homo sapiens glyceraldehyde-3-phosphate dehydrogenase (GAPDH), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002046

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGGGAAGG TGAAGGTCGG AGTCAACGGA TTTGGTCGTA TTGGGCGCCT GGTCACCAGG 
GCTGCTTTTA ACTCTGGTAA AGTGGATATT GTTGCCATCA ATGACCCCTT CATTGACCTC
AACTACATGG TTTACATGTT CCAATATGAT TCCACCCATG GCAAATTCCA TGGCACCGTC
AAGGCTGAGA ACGGGAAGCT TGTCATCAAT GGAAATCCCA TCACCATCTT CCAGGAGCGA
GATCCCTCCA AAATCAAGTG GGGCGATGCT GGCGCTGAGT ACGTCGTGGA GTCCACTGGC
GTCTTCACCA CCATGGAGAA GGCTGGGGCT CATTTGCAGG GGGGAGCCAA AAGGGTCATC
ATCTCTGCCC CCTCTGCTGA TGCCCCCATG TTCGTCATGG GTGTGAACCA TGAGAAGTAT
GACAACAGCC TCAAGATCAT CAGCAATGCC TCCTGCACCA CCAACTGCTT AGCACCCCTG
GCCAAGGTCA TCCATGACAA CTTTGGTATC GTGGAAGGAC TCATGACCAC AGTCCATGCC
ATCACTGCCA CCCAGAAGAC TGTGGATGGC CCCTCCGGGA AACTGTGGCG TGATGGCCGC
GGGGCTCTCC AGAACATCAT CCCTGCCTCT ACTGGCGCTG CCAAGGCTGT GGGCAAGGTC
ATCCCTGAGC TGAACGGGAA GCTCACTGGC ATGGCCTTCC GTGTCCCCAC TGCCAACGTG
TCAGTGGTGG ACCTGACCTG CCGTCTAGAA AAACCTGCCA AATATGATGA CATCAAGAAG
GTGGTGAAGC AGGCGTCGGA GGGCCCCCTC AAGGGCATCC TGGGCTACAC TGAGCACCAG
GTGGTCTCCT CTGACTTCAA CAGCGACACC CACTCCTCCA CCTTTGACGC TGGGGCTGGC
ATTGCCCTCA ACGACCACTT TGTCAAGCTC ATTTCCTGGT ATGACAACGA ATTTGGCTAC
AGCAACAGGG TGGTGGACCT CATGGCCCAC ATGGCCTCCA AGGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20465
Clone ID Related Accession (Same CDS sequence) NM_001256799
Accession Version NM_001256799.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 882bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product glyceraldehyde-3-phosphate dehydrogenase isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY000136.1, AF261085.1 and BE893087.1. On Jan 28, 2014 this sequence version replaced gi:378404907. Transcript Variant: This variant (2) differs in the 5' UTR and coding region compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (2) with a shorter N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## multifunctional gene product(s) :: PMID: 20727968 ##RefSeq-Attributes-END## ##Evidence-Data-START## CDS exon combination :: BC029340.1, BM478815.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGTTTACA TGTTCCAATA TGATTCCACC CATGGCAAAT TCCATGGCAC CGTCAAGGCT 
GAGAACGGGA AGCTTGTCAT CAATGGAAAT CCCATCACCA TCTTCCAGGA GCGAGATCCC
TCCAAAATCA AGTGGGGCGA TGCTGGCGCT GAGTACGTCG TGGAGTCCAC TGGCGTCTTC
ACCACCATGG AGAAGGCTGG GGCTCATTTG CAGGGGGGAG CCAAAAGGGT CATCATCTCT
GCCCCCTCTG CTGATGCCCC CATGTTCGTC ATGGGTGTGA ACCATGAGAA GTATGACAAC
AGCCTCAAGA TCATCAGCAA TGCCTCCTGC ACCACCAACT GCTTAGCACC CCTGGCCAAG
GTCATCCATG ACAACTTTGG TATCGTGGAA GGACTCATGA CCACAGTCCA TGCCATCACT
GCCACCCAGA AGACTGTGGA TGGCCCCTCC GGGAAACTGT GGCGTGATGG CCGCGGGGCT
CTCCAGAACA TCATCCCTGC CTCTACTGGC GCTGCCAAGG CTGTGGGCAA GGTCATCCCT
GAGCTGAACG GGAAGCTCAC TGGCATGGCC TTCCGTGTCC CCACTGCCAA CGTGTCAGTG
GTGGACCTGA CCTGCCGTCT AGAAAAACCT GCCAAATATG ATGACATCAA GAAGGTGGTG
AAGCAGGCGT CGGAGGGCCC CCTCAAGGGC ATCCTGGGCT ACACTGAGCA CCAGGTGGTC
TCCTCTGACT TCAACAGCGA CACCCACTCC TCCACCTTTG ACGCTGGGGC TGGCATTGCC
CTCAACGACC ACTTTGTCAA GCTCATTTCC TGGTATGACA ACGAATTTGG CTACAGCAAC
AGGGTGGTGG ACCTCATGGC CCACATGGCC TCCAAGGAGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001243728.1
CDS349..1230
Misc Feature(1)250..252(+)
Misc Feature(2)349..678(+)
Misc Feature(3)691..1164(+)
Exon (1)1..251
Gene:GAPDH
Gene Synonym:
Exon (2)252..351
Gene:GAPDH
Gene Synonym:
Exon (3)352..458
Gene:GAPDH
Gene Synonym:
Exon (4)459..549
Gene:GAPDH
Gene Synonym:
Exon (5)550..665
Gene:GAPDH
Gene Synonym:
Exon (6)666..747
Gene:GAPDH
Gene Synonym:
Exon (7)748..1160
Gene:GAPDH
Gene Synonym:
Exon (8)1161..1435
Gene:GAPDH
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001256799
Organism Homo sapiens (human)
Definition Homo sapiens glyceraldehyde-3-phosphate dehydrogenase (GAPDH), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001256799

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGTTTACA TGTTCCAATA TGATTCCACC CATGGCAAAT TCCATGGCAC CGTCAAGGCT 
GAGAACGGGA AGCTTGTCAT CAATGGAAAT CCCATCACCA TCTTCCAGGA GCGAGATCCC
TCCAAAATCA AGTGGGGCGA TGCTGGCGCT GAGTACGTCG TGGAGTCCAC TGGCGTCTTC
ACCACCATGG AGAAGGCTGG GGCTCATTTG CAGGGGGGAG CCAAAAGGGT CATCATCTCT
GCCCCCTCTG CTGATGCCCC CATGTTCGTC ATGGGTGTGA ACCATGAGAA GTATGACAAC
AGCCTCAAGA TCATCAGCAA TGCCTCCTGC ACCACCAACT GCTTAGCACC CCTGGCCAAG
GTCATCCATG ACAACTTTGG TATCGTGGAA GGACTCATGA CCACAGTCCA TGCCATCACT
GCCACCCAGA AGACTGTGGA TGGCCCCTCC GGGAAACTGT GGCGTGATGG CCGCGGGGCT
CTCCAGAACA TCATCCCTGC CTCTACTGGC GCTGCCAAGG CTGTGGGCAA GGTCATCCCT
GAGCTGAACG GGAAGCTCAC TGGCATGGCC TTCCGTGTCC CCACTGCCAA CGTGTCAGTG
GTGGACCTGA CCTGCCGTCT AGAAAAACCT GCCAAATATG ATGACATCAA GAAGGTGGTG
AAGCAGGCGT CGGAGGGCCC CCTCAAGGGC ATCCTGGGCT ACACTGAGCA CCAGGTGGTC
TCCTCTGACT TCAACAGCGA CACCCACTCC TCCACCTTTG ACGCTGGGGC TGGCATTGCC
CTCAACGACC ACTTTGTCAA GCTCATTTCC TGGTATGACA ACGAATTTGG CTACAGCAAC
AGGGTGGTGG ACCTCATGGC CCACATGGCC TCCAAGGAGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20566
Clone ID Related Accession (Same CDS sequence) NM_001289745 , NM_001289746 , NM_002046
Accession Version NM_001289746.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1008bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 13-MAY-2015
Organism Homo sapiens (human)
Product glyceraldehyde-3-phosphate dehydrogenase isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY004110.1, HY022295.1, BC023632.2 and BE893087.1. Transcript Variant: This variant (4) differs in the 5' UTR, compared to variant 1. Variants 1, 3, and 4 encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC023632.2, X01677.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## multifunctional gene product(s) :: PMID: 20727968 Protein has antimicrobial activity :: PMID: 22832495 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGGGAAGG TGAAGGTCGG AGTCAACGGA TTTGGTCGTA TTGGGCGCCT GGTCACCAGG 
GCTGCTTTTA ACTCTGGTAA AGTGGATATT GTTGCCATCA ATGACCCCTT CATTGACCTC
AACTACATGG TTTACATGTT CCAATATGAT TCCACCCATG GCAAATTCCA TGGCACCGTC
AAGGCTGAGA ACGGGAAGCT TGTCATCAAT GGAAATCCCA TCACCATCTT CCAGGAGCGA
GATCCCTCCA AAATCAAGTG GGGCGATGCT GGCGCTGAGT ACGTCGTGGA GTCCACTGGC
GTCTTCACCA CCATGGAGAA GGCTGGGGCT CATTTGCAGG GGGGAGCCAA AAGGGTCATC
ATCTCTGCCC CCTCTGCTGA TGCCCCCATG TTCGTCATGG GTGTGAACCA TGAGAAGTAT
GACAACAGCC TCAAGATCAT CAGCAATGCC TCCTGCACCA CCAACTGCTT AGCACCCCTG
GCCAAGGTCA TCCATGACAA CTTTGGTATC GTGGAAGGAC TCATGACCAC AGTCCATGCC
ATCACTGCCA CCCAGAAGAC TGTGGATGGC CCCTCCGGGA AACTGTGGCG TGATGGCCGC
GGGGCTCTCC AGAACATCAT CCCTGCCTCT ACTGGCGCTG CCAAGGCTGT GGGCAAGGTC
ATCCCTGAGC TGAACGGGAA GCTCACTGGC ATGGCCTTCC GTGTCCCCAC TGCCAACGTG
TCAGTGGTGG ACCTGACCTG CCGTCTAGAA AAACCTGCCA AATATGATGA CATCAAGAAG
GTGGTGAAGC AGGCGTCGGA GGGCCCCCTC AAGGGCATCC TGGGCTACAC TGAGCACCAG
GTGGTCTCCT CTGACTTCAA CAGCGACACC CACTCCTCCA CCTTTGACGC TGGGGCTGGC
ATTGCCCTCA ACGACCACTT TGTCAAGCTC ATTTCCTGGT ATGACAACGA ATTTGGCTAC
AGCAACAGGG TGGTGGACCT CATGGCCCAC ATGGCCTCCA AGGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001276675.1
CDS175..1182
Misc Feature(1)121..123(+)
Misc Feature(2)178..618(+)
Misc Feature(3)181..1179(+)
Misc Feature(4)184..630(+)
Misc Feature(5)187..189(+)
Misc Feature(6)199..201(+)
Misc Feature(7)298..300(+)
Misc Feature(8)355..357(+)
Misc Feature(9)364..366(+)
Misc Feature(10)370..372(+)
Misc Feature(11)382..384(+)
Misc Feature(12)397..399(+)
Misc Feature(13)421..423(+)
Misc Feature(14)538..540(+)
Misc Feature(15)616..618(+)
Misc Feature(16)619..621(+)
Misc Feature(17)625..633(+)
Misc Feature(18)625..627(+)
Misc Feature(19)628..630(+)
Misc Feature(20)628..630(+)
Misc Feature(21)628..630(+)
Misc Feature(22)637..639(+)
Misc Feature(23)643..1116(+)
Misc Feature(24)703..705(+)
Misc Feature(25)709..711(+)
Misc Feature(26)718..720(+)
Misc Feature(27)724..726(+)
Misc Feature(28)754..756(+)
Misc Feature(29)754..756(+)
Misc Feature(30)754..756(+)
Misc Feature(31)805..810(+)
Misc Feature(32)805..807(+)
Misc Feature(33)817..819(+)
Misc Feature(34)817..819(+)
Misc Feature(35)829..831(+)
Misc Feature(36)847..849(+)
Misc Feature(37)853..855(+)
Misc Feature(38)853..855(+)
Misc Feature(39)859..861(+)
Misc Feature(40)883..885(+)
Misc Feature(41)895..897(+)
Misc Feature(42)907..924(+)
Misc Feature(43)913..915(+)
Misc Feature(44)934..936(+)
Misc Feature(45)952..954(+)
Misc Feature(46)961..963(+)
Misc Feature(47)1108..1110(+)
Misc Feature(48)1120..1122(+)
Misc Feature(49)1174..1176(+)
Exon (1)1..203
Gene:GAPDH
Gene Synonym:
Exon (2)204..303
Gene:GAPDH
Gene Synonym:
Exon (3)304..410
Gene:GAPDH
Gene Synonym:
Exon (4)411..501
Gene:GAPDH
Gene Synonym:
Exon (5)502..617
Gene:GAPDH
Gene Synonym:
Exon (6)618..699
Gene:GAPDH
Gene Synonym:
Exon (7)700..1112
Gene:GAPDH
Gene Synonym:
Exon (8)1113..1387
Gene:GAPDH
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001289746
Organism Homo sapiens (human)
Definition Homo sapiens glyceraldehyde-3-phosphate dehydrogenase (GAPDH), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001289746

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGGGAAGG TGAAGGTCGG AGTCAACGGA TTTGGTCGTA TTGGGCGCCT GGTCACCAGG 
GCTGCTTTTA ACTCTGGTAA AGTGGATATT GTTGCCATCA ATGACCCCTT CATTGACCTC
AACTACATGG TTTACATGTT CCAATATGAT TCCACCCATG GCAAATTCCA TGGCACCGTC
AAGGCTGAGA ACGGGAAGCT TGTCATCAAT GGAAATCCCA TCACCATCTT CCAGGAGCGA
GATCCCTCCA AAATCAAGTG GGGCGATGCT GGCGCTGAGT ACGTCGTGGA GTCCACTGGC
GTCTTCACCA CCATGGAGAA GGCTGGGGCT CATTTGCAGG GGGGAGCCAA AAGGGTCATC
ATCTCTGCCC CCTCTGCTGA TGCCCCCATG TTCGTCATGG GTGTGAACCA TGAGAAGTAT
GACAACAGCC TCAAGATCAT CAGCAATGCC TCCTGCACCA CCAACTGCTT AGCACCCCTG
GCCAAGGTCA TCCATGACAA CTTTGGTATC GTGGAAGGAC TCATGACCAC AGTCCATGCC
ATCACTGCCA CCCAGAAGAC TGTGGATGGC CCCTCCGGGA AACTGTGGCG TGATGGCCGC
GGGGCTCTCC AGAACATCAT CCCTGCCTCT ACTGGCGCTG CCAAGGCTGT GGGCAAGGTC
ATCCCTGAGC TGAACGGGAA GCTCACTGGC ATGGCCTTCC GTGTCCCCAC TGCCAACGTG
TCAGTGGTGG ACCTGACCTG CCGTCTAGAA AAACCTGCCA AATATGATGA CATCAAGAAG
GTGGTGAAGC AGGCGTCGGA GGGCCCCCTC AAGGGCATCC TGGGCTACAC TGAGCACCAG
GTGGTCTCCT CTGACTTCAA CAGCGACACC CACTCCTCCA CCTTTGACGC TGGGGCTGGC
ATTGCCCTCA ACGACCACTT TGTCAAGCTC ATTTCCTGGT ATGACAACGA ATTTGGCTAC
AGCAACAGGG TGGTGGACCT CATGGCCCAC ATGGCCTCCA AGGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20566
Clone ID Related Accession (Same CDS sequence) NM_001289745 , NM_001289746 , NM_002046
Accession Version NM_001289745.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1008bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 13-MAY-2015
Organism Homo sapiens (human)
Product glyceraldehyde-3-phosphate dehydrogenase isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY046784.1, BM763361.1, M33197.1 and BE893087.1. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1, 3, and 4 encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC004109.2, BC023632.2 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## multifunctional gene product(s) :: PMID: 20727968 Protein has antimicrobial activity :: PMID: 22832495 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGGGAAGG TGAAGGTCGG AGTCAACGGA TTTGGTCGTA TTGGGCGCCT GGTCACCAGG 
GCTGCTTTTA ACTCTGGTAA AGTGGATATT GTTGCCATCA ATGACCCCTT CATTGACCTC
AACTACATGG TTTACATGTT CCAATATGAT TCCACCCATG GCAAATTCCA TGGCACCGTC
AAGGCTGAGA ACGGGAAGCT TGTCATCAAT GGAAATCCCA TCACCATCTT CCAGGAGCGA
GATCCCTCCA AAATCAAGTG GGGCGATGCT GGCGCTGAGT ACGTCGTGGA GTCCACTGGC
GTCTTCACCA CCATGGAGAA GGCTGGGGCT CATTTGCAGG GGGGAGCCAA AAGGGTCATC
ATCTCTGCCC CCTCTGCTGA TGCCCCCATG TTCGTCATGG GTGTGAACCA TGAGAAGTAT
GACAACAGCC TCAAGATCAT CAGCAATGCC TCCTGCACCA CCAACTGCTT AGCACCCCTG
GCCAAGGTCA TCCATGACAA CTTTGGTATC GTGGAAGGAC TCATGACCAC AGTCCATGCC
ATCACTGCCA CCCAGAAGAC TGTGGATGGC CCCTCCGGGA AACTGTGGCG TGATGGCCGC
GGGGCTCTCC AGAACATCAT CCCTGCCTCT ACTGGCGCTG CCAAGGCTGT GGGCAAGGTC
ATCCCTGAGC TGAACGGGAA GCTCACTGGC ATGGCCTTCC GTGTCCCCAC TGCCAACGTG
TCAGTGGTGG ACCTGACCTG CCGTCTAGAA AAACCTGCCA AATATGATGA CATCAAGAAG
GTGGTGAAGC AGGCGTCGGA GGGCCCCCTC AAGGGCATCC TGGGCTACAC TGAGCACCAG
GTGGTCTCCT CTGACTTCAA CAGCGACACC CACTCCTCCA CCTTTGACGC TGGGGCTGGC
ATTGCCCTCA ACGACCACTT TGTCAAGCTC ATTTCCTGGT ATGACAACGA ATTTGGCTAC
AGCAACAGGG TGGTGGACCT CATGGCCCAC ATGGCCTCCA AGGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001276674.1
CDS281..1288
Misc Feature(1)197..199(+)
Misc Feature(2)284..724(+)
Misc Feature(3)287..1285(+)
Misc Feature(4)290..736(+)
Misc Feature(5)293..295(+)
Misc Feature(6)305..307(+)
Misc Feature(7)404..406(+)
Misc Feature(8)461..463(+)
Misc Feature(9)470..472(+)
Misc Feature(10)476..478(+)
Misc Feature(11)488..490(+)
Misc Feature(12)503..505(+)
Misc Feature(13)527..529(+)
Misc Feature(14)644..646(+)
Misc Feature(15)722..724(+)
Misc Feature(16)725..727(+)
Misc Feature(17)731..739(+)
Misc Feature(18)731..733(+)
Misc Feature(19)734..736(+)
Misc Feature(20)734..736(+)
Misc Feature(21)734..736(+)
Misc Feature(22)743..745(+)
Misc Feature(23)749..1222(+)
Misc Feature(24)809..811(+)
Misc Feature(25)815..817(+)
Misc Feature(26)824..826(+)
Misc Feature(27)830..832(+)
Misc Feature(28)860..862(+)
Misc Feature(29)860..862(+)
Misc Feature(30)860..862(+)
Misc Feature(31)911..916(+)
Misc Feature(32)911..913(+)
Misc Feature(33)923..925(+)
Misc Feature(34)923..925(+)
Misc Feature(35)935..937(+)
Misc Feature(36)953..955(+)
Misc Feature(37)959..961(+)
Misc Feature(38)959..961(+)
Misc Feature(39)965..967(+)
Misc Feature(40)989..991(+)
Misc Feature(41)1001..1003(+)
Misc Feature(42)1013..1030(+)
Misc Feature(43)1019..1021(+)
Misc Feature(44)1040..1042(+)
Misc Feature(45)1058..1060(+)
Misc Feature(46)1067..1069(+)
Misc Feature(47)1214..1216(+)
Misc Feature(48)1226..1228(+)
Misc Feature(49)1280..1282(+)
Exon (1)1..257
Gene:GAPDH
Gene Synonym:
Exon (2)258..309
Gene:GAPDH
Gene Synonym:
Exon (3)310..409
Gene:GAPDH
Gene Synonym:
Exon (4)410..516
Gene:GAPDH
Gene Synonym:
Exon (5)517..607
Gene:GAPDH
Gene Synonym:
Exon (6)608..723
Gene:GAPDH
Gene Synonym:
Exon (7)724..805
Gene:GAPDH
Gene Synonym:
Exon (8)806..1218
Gene:GAPDH
Gene Synonym:
Exon (9)1219..1493
Gene:GAPDH
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001289745
Organism Homo sapiens (human)
Definition Homo sapiens glyceraldehyde-3-phosphate dehydrogenase (GAPDH), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001289745

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGGGAAGG TGAAGGTCGG AGTCAACGGA TTTGGTCGTA TTGGGCGCCT GGTCACCAGG 
GCTGCTTTTA ACTCTGGTAA AGTGGATATT GTTGCCATCA ATGACCCCTT CATTGACCTC
AACTACATGG TTTACATGTT CCAATATGAT TCCACCCATG GCAAATTCCA TGGCACCGTC
AAGGCTGAGA ACGGGAAGCT TGTCATCAAT GGAAATCCCA TCACCATCTT CCAGGAGCGA
GATCCCTCCA AAATCAAGTG GGGCGATGCT GGCGCTGAGT ACGTCGTGGA GTCCACTGGC
GTCTTCACCA CCATGGAGAA GGCTGGGGCT CATTTGCAGG GGGGAGCCAA AAGGGTCATC
ATCTCTGCCC CCTCTGCTGA TGCCCCCATG TTCGTCATGG GTGTGAACCA TGAGAAGTAT
GACAACAGCC TCAAGATCAT CAGCAATGCC TCCTGCACCA CCAACTGCTT AGCACCCCTG
GCCAAGGTCA TCCATGACAA CTTTGGTATC GTGGAAGGAC TCATGACCAC AGTCCATGCC
ATCACTGCCA CCCAGAAGAC TGTGGATGGC CCCTCCGGGA AACTGTGGCG TGATGGCCGC
GGGGCTCTCC AGAACATCAT CCCTGCCTCT ACTGGCGCTG CCAAGGCTGT GGGCAAGGTC
ATCCCTGAGC TGAACGGGAA GCTCACTGGC ATGGCCTTCC GTGTCCCCAC TGCCAACGTG
TCAGTGGTGG ACCTGACCTG CCGTCTAGAA AAACCTGCCA AATATGATGA CATCAAGAAG
GTGGTGAAGC AGGCGTCGGA GGGCCCCCTC AAGGGCATCC TGGGCTACAC TGAGCACCAG
GTGGTCTCCT CTGACTTCAA CAGCGACACC CACTCCTCCA CCTTTGACGC TGGGGCTGGC
ATTGCCCTCA ACGACCACTT TGTCAAGCTC ATTTCCTGGT ATGACAACGA ATTTGGCTAC
AGCAACAGGG TGGTGGACCT CATGGCCCAC ATGGCCTCCA AGGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

G3BP1, G3BP2 and CAPRIN1 are required for translation of interferon stimulated mRNAs and are targeted by a dengue virus non-coding RNA
PLoS Pathog. 10 (7), E1004242 (2014)
Bidet K, Dadlani D and Garcia-Blanco MA.


book

Transglutaminase 2-dependent deamidation of glyceraldehyde-3-phosphate dehydrogenase promotes trophoblastic cell fusion
J. Biol. Chem. 289 (8), 4989-4999 (2014)
Iwai K, Shibukawa Y, Yamazaki N and Wada Y.


book

Glyceraldehyde-3-phosphate dehydrogenase is activated by lysine 254 acetylation in response to glucose signal
J. Biol. Chem. 289 (6), 3775-3785 (2014)
Li T, Liu M, Feng X, Wang Z, Das I, Xu Y, Zhou X, Sun Y, Guan KL, Xiong Y and Lei QY.


book

Mitochondrial disulfide relay mediates translocation of p53 and partitions its subcellular activity
Proc. Natl. Acad. Sci. U.S.A. 110 (43), 17356-17361 (2013)
Zhuang J, Wang PY, Huang X, Chen X, Kang JG and Hwang PM.


book

A peptide derived from the highly conserved protein GAPDH is involved in tissue protection by different antifungal strategies and epithelial immunomodulation
J. Invest. Dermatol. 133 (1), 144-153 (2013)
Wagener J, Schneider JJ, Baxmann S, Kalbacher H, Borelli C, Nuding S, Kuchler R, Wehkamp J, Kaeser MD, Mailander-Sanchez D, Braunsdorf C, Hube B, Schild L, Forssmann WG, Korting HC, Liepke C and Schaller M.


book

The diverse functions of GAPDH: views from different subcellular compartments
Cell. Signal. 23 (2), 317-323 (2011)
Tristan C, Shahani N, Sedlak TW and Sawa A.


book

Treatment of Haemophilus aphrophilus endocarditis with ciprofloxacin
J. Infect. 24 (3), 317-320 (1992)
Dawson SJ and White LA.


book

A human nuclear uracil DNA glycosylase is the 37-kDa subunit of glyceraldehyde-3-phosphate dehydrogenase
Proc. Natl. Acad. Sci. U.S.A. 88 (19), 8460-8464 (1991)
Meyer-Siegler K, Mauro DJ, Seal G, Wurzer J, deRiel JK and Sirover MA.


book

Twinning in crystals of human skeletal muscle D-glyceraldehyde-3-phosphate dehydrogenase
J. Mol. Biol. 104 (1), 277-283 (1976)
Mercer,W.D., Winn,S.I. and Watson,H.C.


book

The covalent structure of glyceraldehyde-phosphate dehydrogenase from human muscles. Isolation and amino acid sequences of peptides from tryptic digest
Hoppe-Seyler's Z. Physiol. Chem. 356 (7), 1181-1183 (1975)
Nowak,K., Kuczek,M., Ostropolska,L., Malarska,A., Wolny,M. and Baranowski,T.


 
*
*
*
*