TMEM97 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TMEM97
Entrez Gene ID	27346
Full Name	transmembrane protein 97
Synonyms	MAC30
General protein information	Preferred Names transmembrane protein 97 Names sigma intracellular receptor 2 transmembrane protein 97 meningioma-associated protein 30 sigma-2 receptor sigma2 receptor
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q11.2
Summary	TMEM97 is a conserved integral membrane protein that plays a role in controlling cellular cholesterol levels (Bartz et al., 2009 [PubMed 19583955]).[supplied by OMIM, Aug 2009].
Disorder MIM:	612912

mRNA and Protein(s)

mRNA	Protein	Name
NM_014573.3	NP_055388.2	sigma intracellular receptor 2
XM_005257965.3	XP_005258022.1	sigma intracellular receptor 2 isoform X1
NM_014573.2	NP_055388.2	sigma intracellular receptor 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	TMEM97	NP_055388.2
	TMEM97	XP_001106505.1
Bos taurus (cattle)	TMEM97	NP_001029575.1
Danio rerio (zebrafish)	LOC569147	XP_697605.1
Pan troglodytes (chimpanzee)	TMEM97	NP_001238843.1
Mus musculus (house mouse)	Tmem97	NP_598467.1
Rattus norvegicus (Norway rat)	Tmem97	NP_001008335.1
Gallus gallus (chicken)	TMEM97	XP_415916.1
Xenopus tropicalis (tropical clawed frog)	tmem97	NP_001005014.1
Canis lupus familiaris (dog)	TMEM97	XP_853562.2

Gene Ontology
Bibliography

Related articles in PubMed

The prognostic role of MAC30 in advanced gastric cancer patients receiving platinum-based chemotherapy.
Wu X, Zhou F, Ji X, Ren K, Shan Y, Mao X, Fen Y, Chen R, Ding H, Fu X
Future oncology (London, England)13(29)2691-2696(2017 Dec)

Sigma 2 Receptor/Tmem97 Agonists Produce Long Lasting Antineuropathic Pain Effects in Mice.
Sahn JJ, Mejia GL, Ray PR, Martin SF, Price TJ
ACS chemical neuroscience8(8)1801-1811(2017 Aug)

Identification of the gene that codes for the ?
Alon A, Schmidt HR, Wood MD, Sahn JJ, Martin SF, Kruse AC
Proceedings of the National Academy of Sciences of the United States of America114(27)7160-7165(2017 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study generated a series of sigma1R and sigma2R/Tmem97 agonists and antagonists and tested them for efficacy in the mouse spared nerve injury model. Results show robust antineuropathic pain effects of sigma1R and sigma2R/Tmem97 ligands, demonstrate that sigma2R/Tmem97 is a novel neuropathic pain target, and identify sigma2R/Tmem97 agonist UKH-1114 as a lead molecule for further development.
Title: Sigma 2 Receptor/Tmem97 Agonists Produce Long Lasting Antineuropathic Pain Effects in Mice.

knockdown of TMEM97 also increases levels of residual NPC1 in NPC1-mutant patient fibroblasts and reduces cholesterol storage in an NPC1-dependent manner. Our findings propose TMEM97 inhibition as a novel strategy to increase residual NPC1 levels in cells and a potential therapeutic target for Niemann-Pick type C disease (NP-C).
Title: Reduction of TMEM97 increases NPC1 protein levels and restores cholesterol trafficking in Niemann-pick type C1 disease cells.

Overexpression of MAC30 is associated with Squamous Cell Carcinomas of the Lung.
Title: Prognostic Value of MAC30 Expression in Human Pure Squamous Cell Carcinomas of the Lung.

High expression of TMEM97 is associated with glioma.
Title: RNA interference against TMEM97 inhibits cell proliferation, migration, and invasion in glioma cells.

The down-regulation of MAC30 expression efficiently inhibited the proliferation of gastric cancer cells. Furthermore, the mobility of gastric cancer cells was also inhibited by down-regulation of MAC30.
Title: Down-regulated MAC30 expression inhibits proliferation and mobility of human gastric cancer cells.

The following TMEM97 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TMEM97 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu30008	NM_014573.3 Latest version!	Homo sapiens transmembrane protein 97 (TMEM97), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu50282	XM_005257965.3 Latest version!	Homo sapiens transmembrane protein 97 (TMEM97), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
View Old Accession Versions >>
OHu30008	NM_014573.2	Homo sapiens transmembrane protein 97 (TMEM97), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TMEM97 ( NM_014573.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055388.2 Homo sapiens transmembrane protein 97 (TMEM97), mRNA.

Price & Availability↑

CloneID	OHu30008
Clone ID Related Accession (Same CDS sequence)	NM_014573.3 , NM_014573.2
Accession Version	NM_014573.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	sigma intracellular receptor 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA410183.1, BC091504.1, AC002094.1 and CA441136.1. On Nov 23, 2018 this sequence version replaced NM_014573.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.97499.1, SRR5189661.190685.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000226230.8/ ENSP00000226230.6 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGGGCTC CGGCAACCAG GCGCTGCGTG GAGTGGCTGC TGGGCCTCTA CTTCCTCAGC 
CACATCCCCA TCACCCTGTT CATGGACCTG CAGGCGGTGC TGCCGCGCGA GCTCTACCCA 
GTCGAGTTTA GAAACCTGCT GAAGTGGTAT GCTAAGGAGT TCAAAGACCC ACTGCTACAG 
GAGCCCCCAG CCTGGTTTAA GTCCTTTCTG TTTTGCGAGC TTGTGTTTCA GCTGCCTTTC 
TTTCCCATTG CAACGTATGC CTTCCTCAAA GGAAGCTGCA AGTGGATTCG AACTCCTGCA 
ATCATCTACT CTGTTCACAC CATGACAACC TTAATTCCGA TACTCTCCAC ATTTCTGTTT 
GAGGATTTCT CCAAAGCCAG TGGTTTCAAG GGACAAAGAC CTGAGACTTT GCATGAACGG 
TTAACCCTTG TGTCTGTCTA TGCCCCCTAC TTACTCATCC CATTCATACT TTTAATTTTC 
ATGTTGCGGA GCCCCTACTA CAAGTATGAA GAGAAAAGAA AAAAAAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055388.2
CDS	41..571
Translation	MGAPATRRCVEWLLGLYFLSHIPITLFMDLQAVLPRELYPVEFRNLLKWYAKEFKDPLLQEPPAWFKSFLFCELVFQLPFFPIATYAFLKGSCKWIRTPAIIYSVHTMTTLIPILSTFLFEDFSKASGFKGQRPETLHERLTLVSVYAPYLLIPFILLIFMLRSPYYKYEEKRKKK

Target ORF information:

RefSeq Version	NM_014573.3
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 97 (TMEM97), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014573.3

ORF Insert Sequence:

ATGGGGGCTC CGGCAACCAG GCGCTGCGTG GAGTGGCTGC TGGGCCTCTA CTTCCTCAGC 
CACATCCCCA TCACCCTGTT CATGGACCTG CAGGCGGTGC TGCCGCGCGA GCTCTACCCA 
GTCGAGTTTA GAAACCTGCT GAAGTGGTAT GCTAAGGAGT TCAAAGACCC ACTGCTACAG 
GAGCCCCCAG CCTGGTTTAA GTCCTTTCTG TTTTGCGAGC TTGTGTTTCA GCTGCCTTTC 
TTTCCCATTG CAACGTATGC CTTCCTCAAA GGAAGCTGCA AGTGGATTCG AACTCCTGCA 
ATCATCTACT CTGTTCACAC CATGACAACC TTAATTCCGA TACTCTCCAC ATTTCTGTTT 
GAGGATTTCT CCAAAGCCAG TGGTTTCAAG GGACAAAGAC CTGAGACTTT GCATGAACGG 
TTAACCCTTG TGTCTGTCTA TGCCCCCTAC TTACTCATCC CATTCATACT TTTAATTTTC 
ATGTTGCGGA GCCCCTACTA CAAGTATGAA GAGAAAAGAA AAAAAAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM97 ( NM_014573.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_055388.2 Homo sapiens transmembrane protein 97 (TMEM97), mRNA.

Price & Availability↑

CloneID	OHu30008
Clone ID Related Accession (Same CDS sequence)	NM_014573.3 , NM_014573.2
Accession Version	NM_014573.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-28
Organism	Homo sapiens(human)
Product	sigma intracellular receptor 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA410183.1, BC091504.1, AC002094.1 and CA441136.1. On Jul 1, 2006 this sequence version replaced NM_014573.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DA410183.1, SRR1660809.247290.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGGCTC CGGCAACCAG GCGCTGCGTG GAGTGGCTGC TGGGCCTCTA CTTCCTCAGC 
CACATCCCCA TCACCCTGTT CATGGACCTG CAGGCGGTGC TGCCGCGCGA GCTCTACCCA 
GTCGAGTTTA GAAACCTGCT GAAGTGGTAT GCTAAGGAGT TCAAAGACCC ACTGCTACAG 
GAGCCCCCAG CCTGGTTTAA GTCCTTTCTG TTTTGCGAGC TTGTGTTTCA GCTGCCTTTC 
TTTCCCATTG CAACGTATGC CTTCCTCAAA GGAAGCTGCA AGTGGATTCG AACTCCTGCA 
ATCATCTACT CTGTTCACAC CATGACAACC TTAATTCCGA TACTCTCCAC ATTTCTGTTT 
GAGGATTTCT CCAAAGCCAG TGGTTTCAAG GGACAAAGAC CTGAGACTTT GCATGAACGG 
TTAACCCTTG TGTCTGTCTA TGCCCCCTAC TTACTCATCC CATTCATACT TTTAATTTTC 
ATGTTGCGGA GCCCCTACTA CAAGTATGAA GAGAAAAGAA AAAAAAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055388.2
CDS	146..676
Translation	MGAPATRRCVEWLLGLYFLSHIPITLFMDLQAVLPRELYPVEFRNLLKWYAKEFKDPLLQEPPAWFKSFLFCELVFQLPFFPIATYAFLKGSCKWIRTPAIIYSVHTMTTLIPILSTFLFEDFSKASGFKGQRPETLHERLTLVSVYAPYLLIPFILLIFMLRSPYYKYEEKRKKK

Target ORF information:

RefSeq Version	NM_014573.2
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 97 (TMEM97), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014573.2

ORF Insert Sequence:

ATGGGGGCTC CGGCAACCAG GCGCTGCGTG GAGTGGCTGC TGGGCCTCTA CTTCCTCAGC 
CACATCCCCA TCACCCTGTT CATGGACCTG CAGGCGGTGC TGCCGCGCGA GCTCTACCCA 
GTCGAGTTTA GAAACCTGCT GAAGTGGTAT GCTAAGGAGT TCAAAGACCC ACTGCTACAG 
GAGCCCCCAG CCTGGTTTAA GTCCTTTCTG TTTTGCGAGC TTGTGTTTCA GCTGCCTTTC 
TTTCCCATTG CAACGTATGC CTTCCTCAAA GGAAGCTGCA AGTGGATTCG AACTCCTGCA 
ATCATCTACT CTGTTCACAC CATGACAACC TTAATTCCGA TACTCTCCAC ATTTCTGTTT 
GAGGATTTCT CCAAAGCCAG TGGTTTCAAG GGACAAAGAC CTGAGACTTT GCATGAACGG 
TTAACCCTTG TGTCTGTCTA TGCCCCCTAC TTACTCATCC CATTCATACT TTTAATTTTC 
ATGTTGCGGA GCCCCTACTA CAAGTATGAA GAGAAAAGAA AAAAAAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM97 ( XM_005257965.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005258022.1 Homo sapiens transmembrane protein 97 (TMEM97), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu50282
Clone ID Related Accession (Same CDS sequence)	XM_005257965.3
Accession Version	XM_005257965.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sigma intracellular receptor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005257965.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCGG TCTGCCTAGC CCTCGCGGGG GCAAGGCGGC GACCGAGGCG CGTGGGTCTG 
GGAAGGCGCG CGGATTTGGC CCCTCTTCTC ACATCAGCGG GTCCAGGCCC AACCGACAGA 
CTATGGGGGC TCCGGCAACC AGGCGCTGCG TGGAGTGGCT GCTGGGCCTC TACTTCCTCA 
GCCACATCCC CATCACCCTG TTCATGGACC TGCAGGCGGT GCTGCCGCGC GAGCTCTACC 
CAGTCGAGAA ACCTGCTGAA GTGGTATGCT AAGGAGTTCA AAGACCCACT GCTACAGGAG 
CCCCCAGCCT GGTTTAAGTC CTTTCTGTTT TGCGAGCTTG TGTTTCAGCT GCCTTTCTTT 
CCCATTGCAA CGTATGCCTT CCTCAAAGGA AGCTGCAAGT GGATTCGAAC TCCTGCAATC 
ATCTACTCTG TTCACACCAT GACAACCTTA ATTCCGATAC TCTCCACATT TCTGTTTGAG 
GATTTCTCCA AAGCCAGTGG TTTCAAGGGA CAAAGACCTG AGACTTTGCA TGAACGGTTA 
ACCCTTGTGT CTGTCTATGC CCCCTACTTA CTCATCCCAT TCATACTTTT AATTTTCATG 
TTGCGGAGCC CCTACTACAA GTATGAAGAG AAAAGAAAAA AAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005258022.1
CDS	78..725
Translation	MEAVCLALAGARRRPRRVGLGRRADLAPLLTSAGPGPTDRLWGLRQPGAAWSGCWASTSSATSPSPCSWTCRRCCRASSTQSRNLLKWYAKEFKDPLLQEPPAWFKSFLFCELVFQLPFFPIATYAFLKGSCKWIRTPAIIYSVHTMTTLIPILSTFLFEDFSKASGFKGQRPETLHERLTLVSVYAPYLLIPFILLIFMLRSPYYKYEEKRKKK

Target ORF information:

RefSeq Version	XM_005257965.3
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 97 (TMEM97), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005257965.3

ORF Insert Sequence:

ATGGAAGCGG TCTGCCTAGC CCTCGCGGGG GCAAGGCGGC GACCGAGGCG CGTGGGTCTG 
GGAAGGCGCG CGGATTTGGC CCCTCTTCTC ACATCAGCGG GTCCAGGCCC AACCGACAGA 
CTATGGGGGC TCCGGCAACC AGGCGCTGCG TGGAGTGGCT GCTGGGCCTC TACTTCCTCA 
GCCACATCCC CATCACCCTG TTCATGGACC TGCAGGCGGT GCTGCCGCGC GAGCTCTACC 
CAGTCGAGAA ACCTGCTGAA GTGGTATGCT AAGGAGTTCA AAGACCCACT GCTACAGGAG 
CCCCCAGCCT GGTTTAAGTC CTTTCTGTTT TGCGAGCTTG TGTTTCAGCT GCCTTTCTTT 
CCCATTGCAA CGTATGCCTT CCTCAAAGGA AGCTGCAAGT GGATTCGAAC TCCTGCAATC 
ATCTACTCTG TTCACACCAT GACAACCTTA ATTCCGATAC TCTCCACATT TCTGTTTGAG 
GATTTCTCCA AAGCCAGTGG TTTCAAGGGA CAAAGACCTG AGACTTTGCA TGAACGGTTA 
ACCCTTGTGT CTGTCTATGC CCCCTACTTA CTCATCCCAT TCATACTTTT AATTTTCATG 
TTGCGGAGCC CCTACTACAA GTATGAAGAG AAAAGAAAAA AAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The prognostic role of MAC30 in advanced gastric cancer patients receiving platinum-based chemotherapy.
Future oncology (London, England)13(29)2691-2696(2017 Dec)
Wu X,Zhou F,Ji X,Ren K,Shan Y,Mao X,Fen Y,Chen R,Ding H,Fu X

Sigma 2 Receptor/Tmem97 Agonists Produce Long Lasting Antineuropathic Pain Effects in Mice.
ACS chemical neuroscience8(8)1801-1811(2017 Aug)
Sahn JJ,Mejia GL,Ray PR,Martin SF,Price TJ

Identification of the gene that codes for the ?
Proceedings of the National Academy of Sciences of the United States of America114(27)7160-7165(2017 Jul)
Alon A,Schmidt HR,Wood MD,Sahn JJ,Martin SF,Kruse AC

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TMEM97 cDNA ORF clone, Homo sapiens(human)

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Related articles in PubMed

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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