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MRPS18B cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol MRPS18B
Entrez Gene ID 28973
Full Name mitochondrial ribosomal protein S18B
Synonyms C6orf14,HSPC183,HumanS18a,MRP-S18-2,MRPS18-2,PTD017,S18amt
General protein information
Preferred Names

mitochondrial ribosomal protein S18B

Names

28S ribosomal protein S18b, mitochondrial
28S ribosomal protein S18-2, mitochondrial
MRP-S18-b
S18mt-b
mitochondrial ribosomal protein S18-2
mitochondrial small ribosomal subunit protein bS18b
mitochondrial small ribosomal subunit protein bS18m-B
mitochondrial small ribosomal subunit protein mS40
mrps18-b
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

6

6p21.33
Summary Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S18P family. The encoded protein is one of three that has significant sequence similarity to bacterial S18 proteins. The primary sequences of the three human mitochondrial S18 proteins are no more closely related to each other than they are to the prokaryotic S18 proteins. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q. [provided by RefSeq, Jul 2008].
Disorder MIM:

611982


Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_024446408.1 XP_024302176.1 28S ribosomal protein S18b, mitochondrial isoform X1
NM_014046.3 NP_054765.1 28S ribosomal protein S18b, mitochondrial
NM_014046.4 NP_054765.1 28S ribosomal protein S18b, mitochondrial

Pathways from BioSystems


Homologies


Pan troglodytes (chimpanzee) MRPS18B NP_001035204.1
MRPS18B NP_001098635.1
Canis lupus familiaris (dog) MRPS18B XP_532057.2
Xenopus tropicalis (tropical clawed frog) LOC100489063 XP_002939509.2
Homo sapiens (human) MRPS18B NP_054765.1
Danio rerio (zebrafish) mrps18b NP_001017759.1
Bos taurus (cattle) MRPS18B NP_001033613.1
Mus musculus (house mouse) Mrps18b NP_080154.1
Rattus norvegicus (Norway rat) Mrps18b NP_997699.2

Gene Ontology


Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Do MRPS18-2 and RB proteins cooperate to control cell stemness and differentiation, preventing cancer development?
Kashuba E, Mushtaq M
Experimental oncology39(1)12-16(2017 Mar)

S18 family of mitochondrial ribosomal proteins: evolutionary history and Gly132 polymorphism in colon carcinoma.
Mushtaq M, Ali RH, Kashuba V, Klein G, Kashuba E
Oncotarget7(34)55649-55662(2016 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Studies suggest that mitochondrial ribosomal protein S18-2 protein, together with the retinoblastoma protein (RB), plays a crucial role in the control on cell stemness and differentiation.
Title: Do MRPS18-2 and RB proteins cooperate to control cell stemness and differentiation, preventing cancer development?

We conclude that high expression of S18-2 and free E2F...1might be a good set of prognostic markers for endometrial cancer
Title: Mitochondrial ribosomal protein S18-2 is highly expressed in endometrial cancers along with free E2F1.

S18-2 is a newly identified oncoprotein that may be involved in cancerogenesis
Title: Mitochondrial ribosomal protein S18-2 evokes chromosomal instability and transforms primary rat skin fibroblasts.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

Observational study of gene-disease association. (HuGE Navigator)
Title: High-density SNP screening of the major histocompatibility complex in systemic lupus erythematosus demonstrates strong evidence for independent susceptibility regions.

The following MRPS18B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MRPS18B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu30577 NM_014046.4
Latest version!		 Homo sapiens mitochondrial ribosomal protein S18B (MRPS18B), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu40273 XM_024446408.1
Latest version!		 Homo sapiens mitochondrial ribosomal protein S18B (MRPS18B), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $139.30
$199.00
View Old Accession Versions >>
OHu30577 NM_014046.3 Homo sapiens mitochondrial ribosomal protein S18B (MRPS18B), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu30577
Clone ID Related Accession (Same CDS sequence) NM_014046.3 , NM_014046.4
Accession Version NM_014046.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 777bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-12-10
Organism Homo sapiens(human)
Product 28S ribosomal protein S18b, mitochondrial
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP369345.1, BG773408.1, AF100761.1 and BE220785.1. On Apr 29, 2008 this sequence version replaced NM_014046.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF151017.1, SRR1163655.53704.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCGGCGT CTGTATTAAA CACCGTGCTG AGGCGGCTTC CTATGCTATC TCTCTTCCGA 
GGTTCTCACA GAGTTCAGGT TCCCCTCCAG ACTCTTTGCA CCAAAGCTCC CTCTGAGGAA 
GATTCTTTGT CCTCAGTTCC CATTTCTCCT TATAAGGATG AGCCCTGGAA ATATCTGGAA 
TCAGAAGAAT ACCAGGAGCG ATATGGTTCT CGCCCCGTCT GGGCTGACTA CCGCCGCAAC 
CACAAGGGTG GTGTACCCCC ACAGCGGACT CGGAAGACAT GTATTCGTCG GAATAAAGTT 
GTTGGGAATC CCTGCCCCAT CTGTCGAGAT CACAAGTTGC ATGTTGACTT TAGGAACGTG 
AAGCTCTTGG AGCAATTTGT CTGCGCCCAC ACGGGTATCA TCTTCTATGC TCCATACACA 
GGAGTCTGTG TGAAGCAGCA CAAGCGGTTG ACCCAGGCCA TCCAGAAAGC CAGGGATCAT 
GGTCTCCTCA TTTACCACAT CCCCCAGGTT GAACCACGGG ACCTTGACTT CAGTACCTCT 
CATGGGGCTG TGAGTGCTAC TCCGCCAGCC CCCACCCTGG TCTCAGGTGA CCCCTGGTAC 
CCATGGTACA ACTGGAAACA GCCACCGGAG AGAGAACTGT CTCGCCTTCG CCGGCTTTAC 
CAGGGTCATC TCCAAGAAGA GAGTGGCCCC CCACCTGAGT CAATGCCCAA GATGCCCCCT 
AGAACACCAG CGGAAGCCTC CTCCACTGGG CAGACAGGCC CTCAGAGTGC TCTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_054765.1
CDS158..934
Translation

Target ORF information:

RefSeq Version NM_014046.3
Organism Homo sapiens(human)
Definition Homo sapiens mitochondrial ribosomal protein S18B (MRPS18B), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_014046.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCGGCGT CTGTATTAAA CACCGTGCTG AGGCGGCTTC CTATGCTATC TCTCTTCCGA 
GGTTCTCACA GAGTTCAGGT TCCCCTCCAG ACTCTTTGCA CCAAAGCTCC CTCTGAGGAA 
GATTCTTTGT CCTCAGTTCC CATTTCTCCT TATAAGGATG AGCCCTGGAA ATATCTGGAA 
TCAGAAGAAT ACCAGGAGCG ATATGGTTCT CGCCCCGTCT GGGCTGACTA CCGCCGCAAC 
CACAAGGGTG GTGTACCCCC ACAGCGGACT CGGAAGACAT GTATTCGTCG GAATAAAGTT 
GTTGGGAATC CCTGCCCCAT CTGTCGAGAT CACAAGTTGC ATGTTGACTT TAGGAACGTG 
AAGCTCTTGG AGCAATTTGT CTGCGCCCAC ACGGGTATCA TCTTCTATGC TCCATACACA 
GGAGTCTGTG TGAAGCAGCA CAAGCGGTTG ACCCAGGCCA TCCAGAAAGC CAGGGATCAT 
GGTCTCCTCA TTTACCACAT CCCCCAGGTT GAACCACGGG ACCTTGACTT CAGTACCTCT 
CATGGGGCTG TGAGTGCTAC TCCGCCAGCC CCCACCCTGG TCTCAGGTGA CCCCTGGTAC 
CCATGGTACA ACTGGAAACA GCCACCGGAG AGAGAACTGT CTCGCCTTCG CCGGCTTTAC 
CAGGGTCATC TCCAAGAAGA GAGTGGCCCC CCACCTGAGT CAATGCCCAA GATGCCCCCT 
AGAACACCAG CGGAAGCCTC CTCCACTGGG CAGACAGGCC CTCAGAGTGC TCTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu30577
Clone ID Related Accession (Same CDS sequence) NM_014046.3 , NM_014046.4
Accession Version NM_014046.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 777bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-10-09
Organism Homo sapiens(human)
Product 28S ribosomal protein S18b, mitochondrial
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG773408.1, AF100761.1 and BE220785.1. On Nov 23, 2018 this sequence version replaced NM_014046.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.963164.1, AF151017.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000259873.5/ ENSP00000259873.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. 

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCGGCGT CTGTATTAAA CACCGTGCTG AGGCGGCTTC CTATGCTATC TCTCTTCCGA 
GGTTCTCACA GAGTTCAGGT TCCCCTCCAG ACTCTTTGCA CCAAAGCTCC CTCTGAGGAA 
GATTCTTTGT CCTCAGTTCC CATTTCTCCT TATAAGGATG AGCCCTGGAA ATATCTGGAA 
TCAGAAGAAT ACCAGGAGCG ATATGGTTCT CGCCCCGTCT GGGCTGACTA CCGCCGCAAC 
CACAAGGGTG GTGTACCCCC ACAGCGGACT CGGAAGACAT GTATTCGTCG GAATAAAGTT 
GTTGGGAATC CCTGCCCCAT CTGTCGAGAT CACAAGTTGC ATGTTGACTT TAGGAACGTG 
AAGCTCTTGG AGCAATTTGT CTGCGCCCAC ACGGGTATCA TCTTCTATGC TCCATACACA 
GGAGTCTGTG TGAAGCAGCA CAAGCGGTTG ACCCAGGCCA TCCAGAAAGC CAGGGATCAT 
GGTCTCCTCA TTTACCACAT CCCCCAGGTT GAACCACGGG ACCTTGACTT CAGTACCTCT 
CATGGGGCTG TGAGTGCTAC TCCGCCAGCC CCCACCCTGG TCTCAGGTGA CCCCTGGTAC 
CCATGGTACA ACTGGAAACA GCCACCGGAG AGAGAACTGT CTCGCCTTCG CCGGCTTTAC 
CAGGGTCATC TCCAAGAAGA GAGTGGCCCC CCACCTGAGT CAATGCCCAA GATGCCCCCT 
AGAACACCAG CGGAAGCCTC CTCCACTGGG CAGACAGGCC CTCAGAGTGC TCTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_054765.1
CDS27..803
Translation

Target ORF information:

RefSeq Version NM_014046.4
Organism Homo sapiens(human)
Definition Homo sapiens mitochondrial ribosomal protein S18B (MRPS18B), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_014046.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCGGCGT CTGTATTAAA CACCGTGCTG AGGCGGCTTC CTATGCTATC TCTCTTCCGA 
GGTTCTCACA GAGTTCAGGT TCCCCTCCAG ACTCTTTGCA CCAAAGCTCC CTCTGAGGAA 
GATTCTTTGT CCTCAGTTCC CATTTCTCCT TATAAGGATG AGCCCTGGAA ATATCTGGAA 
TCAGAAGAAT ACCAGGAGCG ATATGGTTCT CGCCCCGTCT GGGCTGACTA CCGCCGCAAC 
CACAAGGGTG GTGTACCCCC ACAGCGGACT CGGAAGACAT GTATTCGTCG GAATAAAGTT 
GTTGGGAATC CCTGCCCCAT CTGTCGAGAT CACAAGTTGC ATGTTGACTT TAGGAACGTG 
AAGCTCTTGG AGCAATTTGT CTGCGCCCAC ACGGGTATCA TCTTCTATGC TCCATACACA 
GGAGTCTGTG TGAAGCAGCA CAAGCGGTTG ACCCAGGCCA TCCAGAAAGC CAGGGATCAT 
GGTCTCCTCA TTTACCACAT CCCCCAGGTT GAACCACGGG ACCTTGACTT CAGTACCTCT 
CATGGGGCTG TGAGTGCTAC TCCGCCAGCC CCCACCCTGG TCTCAGGTGA CCCCTGGTAC 
CCATGGTACA ACTGGAAACA GCCACCGGAG AGAGAACTGT CTCGCCTTCG CCGGCTTTAC 
CAGGGTCATC TCCAAGAAGA GAGTGGCCCC CCACCTGAGT CAATGCCCAA GATGCCCCCT 
AGAACACCAG CGGAAGCCTC CTCCACTGGG CAGACAGGCC CTCAGAGTGC TCTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu40273
Clone ID Related Accession (Same CDS sequence) XM_024446408.1
Accession Version XM_024446408.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 603bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product 28S ribosomal protein S18b, mitochondrial isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
ATGCGATCTA AGAGTCGTAG TGACTGGCCA AAAAAAACCG CAATTTTGGG GTCTAATTCG 
ATTGTGACGC AGTTGAAATT AGCTTCTCCC CCATGCCTTC CCTTTCACGC TTCCGTCCTG 
ACGCAAACGT GGGGCCGCCT TCCGCACTGC GGGCTTGTCC TTGGCCCTGC CCTACTCAGT 
TTCCTGAAGC ATGCGCAGTT GCCTTTCCGT CAATTCCTGT CCTGGGCGTA CGTCAAGATG 
GCGGCGTCTG TATTAAACAC CGTGCTGAGG CGGCTTCCTA TGCTATCTCT CTTCCGAGGT 
TCTCACAGAG TTCAGGTTCC CCTCCAGACT CTTTGCACCA AAGCTCCCTC TGAGGAAGAT 
TCTTTGTCCT CAGTTCCCAT TTCTCCTTAT AAGGATGAGC CCTGGAAATA TCTGGAATCA 
GAAGAATACC AGGAGCGATA TGGTTCTCGC CCCGTCTGGG CTGACTACCG CCGCAACCAC 
AAGGGTGGTG TACCCCCACA GCGGACTCGG AAGACATGTA TTCGTCGGAA TAAAGTTGTT 
GGGAATCCCT GCCCCATCTG TCGAGATCAC AAGTTGCATG TTGACTTTAG GGAGTCTGTG 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_024302176.1
CDS310..912
Translation

Target ORF information:

RefSeq Version XM_024446408.1
Organism Homo sapiens(human)
Definition Homo sapiens mitochondrial ribosomal protein S18B (MRPS18B), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_024446408.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGCGATCTA AGAGTCGTAG TGACTGGCCA AAAAAAACCG CAATTTTGGG GTCTAATTCG 
ATTGTGACGC AGTTGAAATT AGCTTCTCCC CCATGCCTTC CCTTTCACGC TTCCGTCCTG 
ACGCAAACGT GGGGCCGCCT TCCGCACTGC GGGCTTGTCC TTGGCCCTGC CCTACTCAGT 
TTCCTGAAGC ATGCGCAGTT GCCTTTCCGT CAATTCCTGT CCTGGGCGTA CGTCAAGATG 
GCGGCGTCTG TATTAAACAC CGTGCTGAGG CGGCTTCCTA TGCTATCTCT CTTCCGAGGT 
TCTCACAGAG TTCAGGTTCC CCTCCAGACT CTTTGCACCA AAGCTCCCTC TGAGGAAGAT 
TCTTTGTCCT CAGTTCCCAT TTCTCCTTAT AAGGATGAGC CCTGGAAATA TCTGGAATCA 
GAAGAATACC AGGAGCGATA TGGTTCTCGC CCCGTCTGGG CTGACTACCG CCGCAACCAC 
AAGGGTGGTG TACCCCCACA GCGGACTCGG AAGACATGTA TTCGTCGGAA TAAAGTTGTT 
GGGAATCCCT GCCCCATCTG TCGAGATCAC AAGTTGCATG TTGACTTTAG GGAGTCTGTG 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW


book

Do MRPS18-2 and RB proteins cooperate to control cell stemness and differentiation, preventing cancer development?
Experimental oncology39(1)12-16(2017 Mar)
Kashuba E,Mushtaq M


book

S18 family of mitochondrial ribosomal proteins: evolutionary history and Gly132 polymorphism in colon carcinoma.
Oncotarget7(34)55649-55662(2016 Aug)
Mushtaq M,Ali RH,Kashuba V,Klein G,Kashuba E