×

GSK3B cDNA ORF clone, Homo sapiens(Human)

Gene Symbol GSK3B
Entrez Gene ID 2932
Full Name glycogen synthase kinase 3 beta
General protein information
Preferred Names

glycogen synthase kinase 3 beta

Names

glycogen synthase kinase-3 beta
GSK-3 beta
GSK3beta isoform
serine/threonine-protein kinase GSK3B

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

3

3q13.33

Summary The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009].
Disorder MIM:

605004


mRNA and Protein(s)

mRNA Protein Name
NM_001146156.1 NP_001139628.1 glycogen synthase kinase-3 beta isoform 2
NM_002093.3 NP_002084.2 glycogen synthase kinase-3 beta isoform 1
XM_006713610.2 XP_006713673.1 glycogen synthase kinase-3 beta isoform X1
XM_006713611.2 XP_006713674.1 glycogen synthase kinase-3 beta isoform X2



Bos taurus (cattle) GSK3B NP_001094780.1
Mus musculus (house mouse) Gsk3b NP_062801.1
Rattus norvegicus (Norway rat) Gsk3b NP_114469.1
Caenorhabditis elegans (roundworm) gsk-3 NP_493243.1
Xenopus tropicalis (tropical clawed frog) gsk3b XP_002941025.2
Pan troglodytes (chimpanzee) GSK3B XP_003339094.1
Canis lupus familiaris (dog) GSK3B XP_535751.2
Danio rerio (zebrafish) gsk3b NP_571456.1
Homo sapiens (human) GSK3B NP_002084.2
Arabidopsis thaliana (thale cress) SK13 NP_196968.2
Gallus gallus (chicken) GSK3B XP_416557.4
Oryza sativa (rice) Os05g0134000 NP_001054564.1


Related articles in PubMed

Role of GSK3β in breast cancer susceptibility.
Aristizabal-Pachon AF, Castillo WO
Cancer biomarkers : section A of Disease markers18(2)169-175(2017)

The gender-specific association of rs334558 in GSK3β with major depressive disorder.
Liu S, Wang L, Sun N, Yang C, Liu Z, Li X, Cao X, Xu Y, Zhang K
Medicine96(3)e5928(2017 Jan)

Up-Regulation of Excitatory Amino Acid Transporters EAAT3 and EAAT4 by Lithium Sensitive Glycogen Synthase Kinase GSK3ß.
Abousaab A, Lang F
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology40(5)1252-1260(2016)

Down-Regulation of the Na+,Cl- Coupled Creatine Transporter CreaT (SLC6A8) by Glycogen Synthase Kinase GSK3ß.
Fezai M, Jemaà M, Fakhri H, Chen H, Elsir B, Pelzl L, Lang F
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology40(5)1231-1238(2016)

Emodin Inhibits the Epithelial to Mesenchymal Transition of Epithelial Ovarian Cancer Cells via ILK/GSK-3
Lu J, Xu Y, Wei X, Zhao Z, Xue J, Liu P
BioMed research international20166253280(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We initially observed that p-Akt, p-GSK-3b, and b-catenin were overexpressed in a series of advanced gastric adenocarcinoma specimens and found that the Akt and Wnt pathways were correlated with gastric tumorigenesis. we showed that the PI3K/Akt and Wnt/beta-catenin signaling pathways promoted EMT by epigenetically activating the expression of Twist in gastric cancer.
Title: The Wnt/β-catenin and PI3K/Akt signaling pathways promote EMT in gastric cancer by epigenetic regulation via H3 lysine 27 acetylation.

the signaling pathway study confirmed that Sox9 protected MRC-5 from lipopolysaccharide injury through the AKT/GSK3beta pathway.
Title: Sox9 Protects against Human Lung Fibroblast Cell Apoptosis Induced by LPS through Activation of the AKT/GSK3β Pathway.

High GSK3B expression is associated with hepatoma.
Title: Astragaloside IV inhibits metastasis in hepatoma cells through the suppression of epithelial-mesenchymal transition via the Akt/GSK-3β/β-catenin pathway.

Low expression of GSK3B is associated with oral cancer.
Title: Reversion-inducing cysteine-rich protein with Kazal motifs and its regulation by glycogen synthase kinase 3 signaling in oral cancer.

miR-1303 promotes neuroblastoma (NB) proliferation by targeting GSK3beta and SFRP1, and might be a target for NB therapy.
Title: miR-1303 promotes the proliferation of neuroblastoma cell SH-SY5Y by targeting GSK3β and SFRP1.

The following GSK3B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GSK3B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

Up to 50% OFF! Starting
from $49/clone

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu22830 NM_001146156.1
Latest version!
Homo sapiens glycogen synthase kinase 3 beta (GSK3B), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu23207 NM_002093.3
Latest version!
Homo sapiens glycogen synthase kinase 3 beta (GSK3B), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu37910 XM_006713610.2
Latest version!
Homo sapiens glycogen synthase kinase 3 beta (GSK3B), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu37911 XM_006713611.2
Latest version!
Homo sapiens glycogen synthase kinase 3 beta (GSK3B), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu22830
Clone ID Related Accession (Same CDS sequence) NM_001146156.1
Accession Version NM_001146156.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1263bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-30
Organism Homo sapiens(Human)
Product glycogen synthase kinase-3 beta isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC012760.2, DA477189.1, AC069444.17 and CA312249.1. Transcript Variant: This variant (2) is missing an in-frame coding exon compared to variant 1, resulting in a shorter isoform (2) lacking a 13 aa segment compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC012760.2, SRR1803614.31835.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGTCAGGGC GGCCCAGAAC CACCTCCTTT GCGGAGAGCT GCAAGCCGGT GCAGCAGCCT 
TCAGCTTTTG GCAGCATGAA AGTTAGCAGA GACAAGGACG GCAGCAAGGT GACAACAGTG
GTGGCAACTC CTGGGCAGGG TCCAGACAGG CCACAAGAAG TCAGCTATAC AGACACTAAA
GTGATTGGAA ATGGATCATT TGGTGTGGTA TATCAAGCCA AACTTTGTGA TTCAGGAGAA
CTGGTCGCCA TCAAGAAAGT ATTGCAGGAC AAGAGATTTA AGAATCGAGA GCTCCAGATC
ATGAGAAAGC TAGATCACTG TAACATAGTC CGATTGCGTT ATTTCTTCTA CTCCAGTGGT
GAGAAGAAAG ATGAGGTCTA TCTTAATCTG GTGCTGGACT ATGTTCCGGA AACAGTATAC
AGAGTTGCCA GACACTATAG TCGAGCCAAA CAGACGCTCC CTGTGATTTA TGTCAAGTTG
TATATGTATC AGCTGTTCCG AAGTTTAGCC TATATCCATT CCTTTGGAAT CTGCCATCGG
GATATTAAAC CGCAGAACCT CTTGTTGGAT CCTGATACTG CTGTATTAAA ACTCTGTGAC
TTTGGAAGTG CAAAGCAGCT GGTCCGAGGA GAACCCAATG TTTCGTATAT CTGTTCTCGG
TACTATAGGG CACCAGAGTT GATCTTTGGA GCCACTGATT ATACCTCTAG TATAGATGTA
TGGTCTGCTG GCTGTGTGTT GGCTGAGCTG TTACTAGGAC AACCAATATT TCCAGGGGAT
AGTGGTGTGG ATCAGTTGGT AGAAATAATC AAGGTCCTGG GAACTCCAAC AAGGGAGCAA
ATCAGAGAAA TGAACCCAAA CTACACAGAA TTTAAATTCC CTCAAATTAA GGCACATCCT
TGGACTAAGG TCTTCCGACC CCGAACTCCA CCGGAGGCAA TTGCACTGTG TAGCCGTCTG
CTGGAGTATA CACCAACTGC CCGACTAACA CCACTGGAAG CTTGTGCACA TTCATTTTTT
GATGAATTAC GGGACCCAAA TGTCAAACTA CCAAATGGGC GAGACACACC TGCACTCTTC
AACTTCACCA CTCAAGAACT GTCAAGTAAT CCACCTCTGG CTACCATCCT TATTCCTCCT
CATGCTCGGA TTCAAGCAGC TGCTTCAACC CCCACAAATG CCACAGCAGC GTCAGATGCT
AATACTGGAG ACCGTGGACA GACCAATAAT GCTGCTTCTG CATCAGCTTC CAACTCCACC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001139628.1
CDS984..2246
Misc Feature(1)963..965
Misc Feature(2)1008..1010
Misc Feature(3)1134..2012
Misc Feature(4)1149..2003
Misc Feature(5)order(1167..1181,1191..1193,1230..1232,1236..1238,1269..1271,1311..1313,1377..1388,1395..1397,1401..1406,1524..1526,1530..1532,1536..1541,1545..1547,1581..1583,1590..1592,1623..1625,1629..1640,1644..1646,1758..1760)
Misc Feature(6)order(1167..1178,1191..1193,1230..1232,1236..1238,1311..1313,1377..1388,1395..1397,1530..1532,1536..1541,1545..1547,1581..1583)
Misc Feature(7)order(1179..1184,1617..1643,1665..1670,1761..1775,1779..1784,1794..1796,1854..1865)
Misc Feature(8)order(1269..1271,1401..1403,1524..1526,1530..1532,1590..1592,1623..1625,1629..1640,1644..1646,1758..1760)
Misc Feature(9)order(1578..1610,1614..1646)
Misc Feature(10)1629..1631
Misc Feature(11)order(1665..1667,1770..1775,1779..1784,1791..1796,1854..1871)
Misc Feature(12)2148..2150
Misc Feature(13)2151..2153
Misc Feature(14)2187..2189
Exon (1)1..1071
Exon (2)1072..1265
Exon (3)1266..1349
Exon (4)1350..1460
Exon (5)1461..1591
Exon (6)1592..1698
Exon (7)1699..1796
Exon (8)1797..1892
Exon (9)1893..2079
Exon (10)2080..2178
Exon (11)2179..7081
Translation

Target ORF information:

RefSeq Version NM_001146156.1
Organism Homo sapiens(Human)
Definition Homo sapiens glycogen synthase kinase 3 beta (GSK3B), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001146156.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGTCAGGGC GGCCCAGAAC CACCTCCTTT GCGGAGAGCT GCAAGCCGGT GCAGCAGCCT 
TCAGCTTTTG GCAGCATGAA AGTTAGCAGA GACAAGGACG GCAGCAAGGT GACAACAGTG
GTGGCAACTC CTGGGCAGGG TCCAGACAGG CCACAAGAAG TCAGCTATAC AGACACTAAA
GTGATTGGAA ATGGATCATT TGGTGTGGTA TATCAAGCCA AACTTTGTGA TTCAGGAGAA
CTGGTCGCCA TCAAGAAAGT ATTGCAGGAC AAGAGATTTA AGAATCGAGA GCTCCAGATC
ATGAGAAAGC TAGATCACTG TAACATAGTC CGATTGCGTT ATTTCTTCTA CTCCAGTGGT
GAGAAGAAAG ATGAGGTCTA TCTTAATCTG GTGCTGGACT ATGTTCCGGA AACAGTATAC
AGAGTTGCCA GACACTATAG TCGAGCCAAA CAGACGCTCC CTGTGATTTA TGTCAAGTTG
TATATGTATC AGCTGTTCCG AAGTTTAGCC TATATCCATT CCTTTGGAAT CTGCCATCGG
GATATTAAAC CGCAGAACCT CTTGTTGGAT CCTGATACTG CTGTATTAAA ACTCTGTGAC
TTTGGAAGTG CAAAGCAGCT GGTCCGAGGA GAACCCAATG TTTCGTATAT CTGTTCTCGG
TACTATAGGG CACCAGAGTT GATCTTTGGA GCCACTGATT ATACCTCTAG TATAGATGTA
TGGTCTGCTG GCTGTGTGTT GGCTGAGCTG TTACTAGGAC AACCAATATT TCCAGGGGAT
AGTGGTGTGG ATCAGTTGGT AGAAATAATC AAGGTCCTGG GAACTCCAAC AAGGGAGCAA
ATCAGAGAAA TGAACCCAAA CTACACAGAA TTTAAATTCC CTCAAATTAA GGCACATCCT
TGGACTAAGG TCTTCCGACC CCGAACTCCA CCGGAGGCAA TTGCACTGTG TAGCCGTCTG
CTGGAGTATA CACCAACTGC CCGACTAACA CCACTGGAAG CTTGTGCACA TTCATTTTTT
GATGAATTAC GGGACCCAAA TGTCAAACTA CCAAATGGGC GAGACACACC TGCACTCTTC
AACTTCACCA CTCAAGAACT GTCAAGTAAT CCACCTCTGG CTACCATCCT TATTCCTCCT
CATGCTCGGA TTCAAGCAGC TGCTTCAACC CCCACAAATG CCACAGCAGC GTCAGATGCT
AATACTGGAG ACCGTGGACA GACCAATAAT GCTGCTTCTG CATCAGCTTC CAACTCCACC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23207
Clone ID Related Accession (Same CDS sequence) NM_002093.3
Accession Version NM_002093.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1302bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-30
Organism Homo sapiens(Human)
Product glycogen synthase kinase-3 beta isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC012760.2, DA477189.1, BC000251.1, AC069444.17 and CA312249.1. This sequence is a reference standard in the RefSeqGene project. On Mar 31, 2009 this sequence version replaced NM_002093.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.158949.1, SRR1163655.408116.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGTCAGGGC GGCCCAGAAC CACCTCCTTT GCGGAGAGCT GCAAGCCGGT GCAGCAGCCT 
TCAGCTTTTG GCAGCATGAA AGTTAGCAGA GACAAGGACG GCAGCAAGGT GACAACAGTG
GTGGCAACTC CTGGGCAGGG TCCAGACAGG CCACAAGAAG TCAGCTATAC AGACACTAAA
GTGATTGGAA ATGGATCATT TGGTGTGGTA TATCAAGCCA AACTTTGTGA TTCAGGAGAA
CTGGTCGCCA TCAAGAAAGT ATTGCAGGAC AAGAGATTTA AGAATCGAGA GCTCCAGATC
ATGAGAAAGC TAGATCACTG TAACATAGTC CGATTGCGTT ATTTCTTCTA CTCCAGTGGT
GAGAAGAAAG ATGAGGTCTA TCTTAATCTG GTGCTGGACT ATGTTCCGGA AACAGTATAC
AGAGTTGCCA GACACTATAG TCGAGCCAAA CAGACGCTCC CTGTGATTTA TGTCAAGTTG
TATATGTATC AGCTGTTCCG AAGTTTAGCC TATATCCATT CCTTTGGAAT CTGCCATCGG
GATATTAAAC CGCAGAACCT CTTGTTGGAT CCTGATACTG CTGTATTAAA ACTCTGTGAC
TTTGGAAGTG CAAAGCAGCT GGTCCGAGGA GAACCCAATG TTTCGTATAT CTGTTCTCGG
TACTATAGGG CACCAGAGTT GATCTTTGGA GCCACTGATT ATACCTCTAG TATAGATGTA
TGGTCTGCTG GCTGTGTGTT GGCTGAGCTG TTACTAGGAC AACCAATATT TCCAGGGGAT
AGTGGTGTGG ATCAGTTGGT AGAAATAATC AAGGTCCTGG GAACTCCAAC AAGGGAGCAA
ATCAGAGAAA TGAACCCAAA CTACACAGAA TTTAAATTCC CTCAAATTAA GGCACATCCT
TGGACTAAGG ATTCGTCAGG AACAGGACAT TTCACCTCAG GAGTGCGGGT CTTCCGACCC
CGAACTCCAC CGGAGGCAAT TGCACTGTGT AGCCGTCTGC TGGAGTATAC ACCAACTGCC
CGACTAACAC CACTGGAAGC TTGTGCACAT TCATTTTTTG ATGAATTACG GGACCCAAAT
GTCAAACTAC CAAATGGGCG AGACACACCT GCACTCTTCA ACTTCACCAC TCAAGAACTG
TCAAGTAATC CACCTCTGGC TACCATCCTT ATTCCTCCTC ATGCTCGGAT TCAAGCAGCT
GCTTCAACCC CCACAAATGC CACAGCAGCG TCAGATGCTA ATACTGGAGA CCGTGGACAG
ACCAATAATG CTGCTTCTGC ATCAGCTTCC AACTCCACCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002084.2
CDS984..2285
Misc Feature(1)963..965
Misc Feature(2)1008..1010
Misc Feature(3)1008..1010
Misc Feature(4)1008..1010
Misc Feature(5)1044..1046
Misc Feature(6)1134..2051
Misc Feature(7)1149..2042
Misc Feature(8)order(1167..1181,1191..1193,1230..1232,1236..1238,1269..1271,1311..1313,1377..1388,1395..1397,1401..1406,1524..1526,1530..1532,1536..1541,1545..1547,1581..1583,1590..1592,1623..1625,1629..1640,1644..1646,1758..1760)
Misc Feature(9)order(1167..1178,1191..1193,1230..1232,1236..1238,1311..1313,1377..1388,1395..1397,1530..1532,1536..1541,1545..1547,1581..1583)
Misc Feature(10)order(1179..1184,1617..1643,1665..1670,1761..1775,1779..1784,1794..1796,1854..1865)
Misc Feature(11)order(1269..1271,1401..1403,1524..1526,1530..1532,1590..1592,1623..1625,1629..1640,1644..1646,1758..1760)
Misc Feature(12)order(1578..1610,1614..1646)
Misc Feature(13)1626..1628
Misc Feature(14)1629..1631
Misc Feature(15)order(1665..1667,1770..1775,1779..1784,1791..1796,1854..1871)
Misc Feature(16)2187..2189
Misc Feature(17)2190..2192
Exon (1)1..1071
Exon (2)1072..1265
Exon (3)1266..1349
Exon (4)1350..1460
Exon (5)1461..1591
Exon (6)1592..1698
Exon (7)1699..1796
Exon (8)1797..1892
Exon (9)1893..1931
Exon (10)1932..2118
Exon (11)2119..2217
Exon (12)2218..7120
Translation

Target ORF information:

RefSeq Version NM_002093.3
Organism Homo sapiens(Human)
Definition Homo sapiens glycogen synthase kinase 3 beta (GSK3B), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002093.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGTCAGGGC GGCCCAGAAC CACCTCCTTT GCGGAGAGCT GCAAGCCGGT GCAGCAGCCT 
TCAGCTTTTG GCAGCATGAA AGTTAGCAGA GACAAGGACG GCAGCAAGGT GACAACAGTG
GTGGCAACTC CTGGGCAGGG TCCAGACAGG CCACAAGAAG TCAGCTATAC AGACACTAAA
GTGATTGGAA ATGGATCATT TGGTGTGGTA TATCAAGCCA AACTTTGTGA TTCAGGAGAA
CTGGTCGCCA TCAAGAAAGT ATTGCAGGAC AAGAGATTTA AGAATCGAGA GCTCCAGATC
ATGAGAAAGC TAGATCACTG TAACATAGTC CGATTGCGTT ATTTCTTCTA CTCCAGTGGT
GAGAAGAAAG ATGAGGTCTA TCTTAATCTG GTGCTGGACT ATGTTCCGGA AACAGTATAC
AGAGTTGCCA GACACTATAG TCGAGCCAAA CAGACGCTCC CTGTGATTTA TGTCAAGTTG
TATATGTATC AGCTGTTCCG AAGTTTAGCC TATATCCATT CCTTTGGAAT CTGCCATCGG
GATATTAAAC CGCAGAACCT CTTGTTGGAT CCTGATACTG CTGTATTAAA ACTCTGTGAC
TTTGGAAGTG CAAAGCAGCT GGTCCGAGGA GAACCCAATG TTTCGTATAT CTGTTCTCGG
TACTATAGGG CACCAGAGTT GATCTTTGGA GCCACTGATT ATACCTCTAG TATAGATGTA
TGGTCTGCTG GCTGTGTGTT GGCTGAGCTG TTACTAGGAC AACCAATATT TCCAGGGGAT
AGTGGTGTGG ATCAGTTGGT AGAAATAATC AAGGTCCTGG GAACTCCAAC AAGGGAGCAA
ATCAGAGAAA TGAACCCAAA CTACACAGAA TTTAAATTCC CTCAAATTAA GGCACATCCT
TGGACTAAGG ATTCGTCAGG AACAGGACAT TTCACCTCAG GAGTGCGGGT CTTCCGACCC
CGAACTCCAC CGGAGGCAAT TGCACTGTGT AGCCGTCTGC TGGAGTATAC ACCAACTGCC
CGACTAACAC CACTGGAAGC TTGTGCACAT TCATTTTTTG ATGAATTACG GGACCCAAAT
GTCAAACTAC CAAATGGGCG AGACACACCT GCACTCTTCA ACTTCACCAC TCAAGAACTG
TCAAGTAATC CACCTCTGGC TACCATCCTT ATTCCTCCTC ATGCTCGGAT TCAAGCAGCT
GCTTCAACCC CCACAAATGC CACAGCAGCG TCAGATGCTA ATACTGGAGA CCGTGGACAG
ACCAATAATG CTGCTTCTGC ATCAGCTTCC AACTCCACCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu37910
Clone ID Related Accession (Same CDS sequence) XM_006713610.2
Accession Version XM_006713610.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1203bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product glycogen synthase kinase-3 beta isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006713610.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTCAGGGC GGCCCAGAAC CACCTCCTTT GCGGAGAGCT GCAAGCCGGT GCAGCAGCCT 
TCAGCTTTTG GCAGCATGAA AGTTAGCAGA GACAAGGACG GCAGCAAGGT GACAACAGTG
GTGGCAACTC CTGGGCAGGG TCCAGACAGG CCACAAGAAG TCAGCTATAC AGACACTAAA
GTGATTGGAA ATGGATCATT TGGTGTGGTA TATCAAGCCA AACTTTGTGA TTCAGGAGAA
CTGGTCGCCA TCAAGAAAGT ATTGCAGGAC AAGAGATTTA AGAATCGAGA GCTCCAGATC
ATGAGAAAGC TAGATCACTG TAACATAGTC CGATTGCGTT ATTTCTTCTA CTCCAGTGGT
GAGAAGAAAG ATGAGGTCTA TCTTAATCTG GTGCTGGACT ATGTTCCGGA AACAGTATAC
AGAGTTGCCA GACACTATAG TCGAGCCAAA CAGACGCTCC CTGTGATTTA TGTCAAGTTG
TATATGTATC AGCTGTTCCG AAGTTTAGCC TATATCCATT CCTTTGGAAT CTGCCATCGG
GATATTAAAC CGCAGAACCT CTTGTTGGAT CCTGATACTG CTGTATTAAA ACTCTGTGAC
TTTGGAAGTG CAAAGCAGCT GGTCCGAGGA GAACCCAATG TTTCGTATAT CTGTTCTCGG
TACTATAGGG CACCAGAGTT GATCTTTGGA GCCACTGATT ATACCTCTAG TATAGATGTA
TGGTCTGCTG GCTGTGTGTT GGCTGAGCTG TTACTAGGAC AACCAATATT TCCAGGGGAT
AGTGGTGTGG ATCAGTTGGT AGAAATAATC AAGGTCCTGG GAACTCCAAC AAGGGAGCAA
ATCAGAGAAA TGAACCCAAA CTACACAGAA TTTAAATTCC CTCAAATTAA GGCACATCCT
TGGACTAAGG ATTCGTCAGG AACAGGACAT TTCACCTCAG GAGTGCGGGT CTTCCGACCC
CGAACTCCAC CGGAGGCAAT TGCACTGTGT AGCCGTCTGC TGGAGTATAC ACCAACTGCC
CGACTAACAC CACTGGAAGC TTGTGCACAT TCATTTTTTG ATGAATTACG GGACCCAAAT
GTCAAACTAC CAAATGGGCG AGACACACCT GCACTCTTCA ACTTCACCAC TCAAGATGCT
AATACTGGAG ACCGTGGACA GACCAATAAT GCTGCTTCTG CATCAGCTTC CAACTCCACC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006713673.1
CDS470..1672
Misc Feature(1)620..1537
Misc Feature(2)635..1528
Misc Feature(3)order(653..667,677..679,716..718,722..724,755..757,797..799,863..874,881..883,887..892,1010..1012,1016..1018,1022..1027,1031..1033,1067..1069,1076..1078,1109..1111,1115..1126,1130..1132,1244..1246)
Misc Feature(4)order(653..664,677..679,716..718,722..724,797..799,863..874,881..883,1016..1018,1022..1027,1031..1033,1067..1069)
Misc Feature(5)order(665..670,1103..1129,1151..1156,1247..1261,1265..1270,1280..1282,1340..1351)
Misc Feature(6)order(755..757,887..889,1010..1012,1016..1018,1076..1078,1109..1111,1115..1126,1130..1132,1244..1246)
Misc Feature(7)order(1064..1096,1100..1132)
Misc Feature(8)order(1151..1153,1256..1261,1265..1270,1277..1282,1340..1357)
Translation

Target ORF information:

RefSeq Version XM_006713610.2
Organism Homo sapiens(Human)
Definition Homo sapiens glycogen synthase kinase 3 beta (GSK3B), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006713610.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTCAGGGC GGCCCAGAAC CACCTCCTTT GCGGAGAGCT GCAAGCCGGT GCAGCAGCCT 
TCAGCTTTTG GCAGCATGAA AGTTAGCAGA GACAAGGACG GCAGCAAGGT GACAACAGTG
GTGGCAACTC CTGGGCAGGG TCCAGACAGG CCACAAGAAG TCAGCTATAC AGACACTAAA
GTGATTGGAA ATGGATCATT TGGTGTGGTA TATCAAGCCA AACTTTGTGA TTCAGGAGAA
CTGGTCGCCA TCAAGAAAGT ATTGCAGGAC AAGAGATTTA AGAATCGAGA GCTCCAGATC
ATGAGAAAGC TAGATCACTG TAACATAGTC CGATTGCGTT ATTTCTTCTA CTCCAGTGGT
GAGAAGAAAG ATGAGGTCTA TCTTAATCTG GTGCTGGACT ATGTTCCGGA AACAGTATAC
AGAGTTGCCA GACACTATAG TCGAGCCAAA CAGACGCTCC CTGTGATTTA TGTCAAGTTG
TATATGTATC AGCTGTTCCG AAGTTTAGCC TATATCCATT CCTTTGGAAT CTGCCATCGG
GATATTAAAC CGCAGAACCT CTTGTTGGAT CCTGATACTG CTGTATTAAA ACTCTGTGAC
TTTGGAAGTG CAAAGCAGCT GGTCCGAGGA GAACCCAATG TTTCGTATAT CTGTTCTCGG
TACTATAGGG CACCAGAGTT GATCTTTGGA GCCACTGATT ATACCTCTAG TATAGATGTA
TGGTCTGCTG GCTGTGTGTT GGCTGAGCTG TTACTAGGAC AACCAATATT TCCAGGGGAT
AGTGGTGTGG ATCAGTTGGT AGAAATAATC AAGGTCCTGG GAACTCCAAC AAGGGAGCAA
ATCAGAGAAA TGAACCCAAA CTACACAGAA TTTAAATTCC CTCAAATTAA GGCACATCCT
TGGACTAAGG ATTCGTCAGG AACAGGACAT TTCACCTCAG GAGTGCGGGT CTTCCGACCC
CGAACTCCAC CGGAGGCAAT TGCACTGTGT AGCCGTCTGC TGGAGTATAC ACCAACTGCC
CGACTAACAC CACTGGAAGC TTGTGCACAT TCATTTTTTG ATGAATTACG GGACCCAAAT
GTCAAACTAC CAAATGGGCG AGACACACCT GCACTCTTCA ACTTCACCAC TCAAGATGCT
AATACTGGAG ACCGTGGACA GACCAATAAT GCTGCTTCTG CATCAGCTTC CAACTCCACC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu37911
Clone ID Related Accession (Same CDS sequence) XM_006713611.2
Accession Version XM_006713611.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1164bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product glycogen synthase kinase-3 beta isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006713611.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGTCAGGGC GGCCCAGAAC CACCTCCTTT GCGGAGAGCT GCAAGCCGGT GCAGCAGCCT 
TCAGCTTTTG GCAGCATGAA AGTTAGCAGA GACAAGGACG GCAGCAAGGT GACAACAGTG
GTGGCAACTC CTGGGCAGGG TCCAGACAGG CCACAAGAAG TCAGCTATAC AGACACTAAA
GTGATTGGAA ATGGATCATT TGGTGTGGTA TATCAAGCCA AACTTTGTGA TTCAGGAGAA
CTGGTCGCCA TCAAGAAAGT ATTGCAGGAC AAGAGATTTA AGAATCGAGA GCTCCAGATC
ATGAGAAAGC TAGATCACTG TAACATAGTC CGATTGCGTT ATTTCTTCTA CTCCAGTGGT
GAGAAGAAAG ATGAGGTCTA TCTTAATCTG GTGCTGGACT ATGTTCCGGA AACAGTATAC
AGAGTTGCCA GACACTATAG TCGAGCCAAA CAGACGCTCC CTGTGATTTA TGTCAAGTTG
TATATGTATC AGCTGTTCCG AAGTTTAGCC TATATCCATT CCTTTGGAAT CTGCCATCGG
GATATTAAAC CGCAGAACCT CTTGTTGGAT CCTGATACTG CTGTATTAAA ACTCTGTGAC
TTTGGAAGTG CAAAGCAGCT GGTCCGAGGA GAACCCAATG TTTCGTATAT CTGTTCTCGG
TACTATAGGG CACCAGAGTT GATCTTTGGA GCCACTGATT ATACCTCTAG TATAGATGTA
TGGTCTGCTG GCTGTGTGTT GGCTGAGCTG TTACTAGGAC AACCAATATT TCCAGGGGAT
AGTGGTGTGG ATCAGTTGGT AGAAATAATC AAGGTCCTGG GAACTCCAAC AAGGGAGCAA
ATCAGAGAAA TGAACCCAAA CTACACAGAA TTTAAATTCC CTCAAATTAA GGCACATCCT
TGGACTAAGG TCTTCCGACC CCGAACTCCA CCGGAGGCAA TTGCACTGTG TAGCCGTCTG
CTGGAGTATA CACCAACTGC CCGACTAACA CCACTGGAAG CTTGTGCACA TTCATTTTTT
GATGAATTAC GGGACCCAAA TGTCAAACTA CCAAATGGGC GAGACACACC TGCACTCTTC
AACTTCACCA CTCAAGATGC TAATACTGGA GACCGTGGAC AGACCAATAA TGCTGCTTCT
GCATCAGCTT CCAACTCCAC CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006713674.1
CDS470..1633
Misc Feature(1)620..1498
Misc Feature(2)635..1489
Misc Feature(3)order(653..667,677..679,716..718,722..724,755..757,797..799,863..874,881..883,887..892,1010..1012,1016..1018,1022..1027,1031..1033,1067..1069,1076..1078,1109..1111,1115..1126,1130..1132,1244..1246)
Misc Feature(4)order(653..664,677..679,716..718,722..724,797..799,863..874,881..883,1016..1018,1022..1027,1031..1033,1067..1069)
Misc Feature(5)order(665..670,1103..1129,1151..1156,1247..1261,1265..1270,1280..1282,1340..1351)
Misc Feature(6)order(755..757,887..889,1010..1012,1016..1018,1076..1078,1109..1111,1115..1126,1130..1132,1244..1246)
Misc Feature(7)order(1064..1096,1100..1132)
Misc Feature(8)order(1151..1153,1256..1261,1265..1270,1277..1282,1340..1357)
Translation

Target ORF information:

RefSeq Version XM_006713611.2
Organism Homo sapiens(Human)
Definition Homo sapiens glycogen synthase kinase 3 beta (GSK3B), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006713611.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGTCAGGGC GGCCCAGAAC CACCTCCTTT GCGGAGAGCT GCAAGCCGGT GCAGCAGCCT 
TCAGCTTTTG GCAGCATGAA AGTTAGCAGA GACAAGGACG GCAGCAAGGT GACAACAGTG
GTGGCAACTC CTGGGCAGGG TCCAGACAGG CCACAAGAAG TCAGCTATAC AGACACTAAA
GTGATTGGAA ATGGATCATT TGGTGTGGTA TATCAAGCCA AACTTTGTGA TTCAGGAGAA
CTGGTCGCCA TCAAGAAAGT ATTGCAGGAC AAGAGATTTA AGAATCGAGA GCTCCAGATC
ATGAGAAAGC TAGATCACTG TAACATAGTC CGATTGCGTT ATTTCTTCTA CTCCAGTGGT
GAGAAGAAAG ATGAGGTCTA TCTTAATCTG GTGCTGGACT ATGTTCCGGA AACAGTATAC
AGAGTTGCCA GACACTATAG TCGAGCCAAA CAGACGCTCC CTGTGATTTA TGTCAAGTTG
TATATGTATC AGCTGTTCCG AAGTTTAGCC TATATCCATT CCTTTGGAAT CTGCCATCGG
GATATTAAAC CGCAGAACCT CTTGTTGGAT CCTGATACTG CTGTATTAAA ACTCTGTGAC
TTTGGAAGTG CAAAGCAGCT GGTCCGAGGA GAACCCAATG TTTCGTATAT CTGTTCTCGG
TACTATAGGG CACCAGAGTT GATCTTTGGA GCCACTGATT ATACCTCTAG TATAGATGTA
TGGTCTGCTG GCTGTGTGTT GGCTGAGCTG TTACTAGGAC AACCAATATT TCCAGGGGAT
AGTGGTGTGG ATCAGTTGGT AGAAATAATC AAGGTCCTGG GAACTCCAAC AAGGGAGCAA
ATCAGAGAAA TGAACCCAAA CTACACAGAA TTTAAATTCC CTCAAATTAA GGCACATCCT
TGGACTAAGG TCTTCCGACC CCGAACTCCA CCGGAGGCAA TTGCACTGTG TAGCCGTCTG
CTGGAGTATA CACCAACTGC CCGACTAACA CCACTGGAAG CTTGTGCACA TTCATTTTTT
GATGAATTAC GGGACCCAAA TGTCAAACTA CCAAATGGGC GAGACACACC TGCACTCTTC
AACTTCACCA CTCAAGATGC TAATACTGGA GACCGTGGAC AGACCAATAA TGCTGCTTCT
GCATCAGCTT CCAACTCCAC CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Role of GSK3β in breast cancer susceptibility.
Cancer biomarkers : section A of Disease markers18(2)169-175(2017)
Aristizabal-Pachon AF,Castillo WO


book

The gender-specific association of rs334558 in GSK3β with major depressive disorder.
Medicine96(3)e5928(2017 Jan)
Liu S,Wang L,Sun N,Yang C,Liu Z,Li X,Cao X,Xu Y,Zhang K


book

Up-Regulation of Excitatory Amino Acid Transporters EAAT3 and EAAT4 by Lithium Sensitive Glycogen Synthase Kinase GSK3ß.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology40(5)1252-1260(2016)
Abousaab A,Lang F


book

Down-Regulation of the Na+,Cl- Coupled Creatine Transporter CreaT (SLC6A8) by Glycogen Synthase Kinase GSK3ß.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology40(5)1231-1238(2016)
Fezai M,Jemaà M,Fakhri H,Chen H,Elsir B,Pelzl L,Lang F


book

Emodin Inhibits the Epithelial to Mesenchymal Transition of Epithelial Ovarian Cancer Cells via ILK/GSK-3
BioMed research international20166253280(2016)
Lu J,Xu Y,Wei X,Zhao Z,Xue J,Liu P


 
*
*
*
*