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GUSB cDNA ORF clone, Homo sapiens (human)

Gene Symbol GUSB
Entrez Gene ID 2990
Full Name glucuronidase, beta
Synonyms BG, MPS7
General protein information
Preferred Names
beta-glucuronidase
Names
beta-glucuronidase
beta-G1
beta-D-glucuronidase
NP_000172.2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

7

7q21.11

Summary This gene encodes a hydrolase that degrades glycosaminoglycans, including heparan sulfate, dermatan sulfate, and chondroitin-4,6-sulfate. The enzyme forms a homotetramer that is localized to the lysosome. Mutations in this gene result in mucopolysaccharidosis type VII. Alternative splicing results in multiple transcript variants. There are many pseudogenes of this locus in the human genome.[provided by RefSeq, May 2014]. lac of sum
Disorder MIM:

611499

Disorder Html: Mucopolysaccharidosis VII, 253220 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_005250297 XP_005250354 beta-glucuronidase isoform X1
XM_011516113 XP_011514415 beta-glucuronidase isoform X2
XM_011516114 XP_011514416 beta-glucuronidase isoform X3
NM_001284290 NP_001271219 beta-glucuronidase isoform 2 precursor
NM_001293104 NP_001280033 beta-glucuronidase isoform 3
NM_001293105 NP_001280034 beta-glucuronidase isoform 4
NM_000181 NP_000172 beta-glucuronidase isoform 1 precursor



Homo sapiens (human) GUSB NP_000172.2
Pan troglodytes (chimpanzee) GUSB XP_001138883.1
Canis lupus familiaris (dog) GUSB NP_001003191.1
Bos taurus (cattle) GUSB NP_001076905.1
Mus musculus (house mouse) Gusb NP_034498.1
Rattus norvegicus (Norway rat) Gusb NP_058711.2
Gallus gallus (chicken) GUSB NP_001034405.1
Danio rerio (zebrafish) LOC100535236 XP_005165070.1
Danio rerio (zebrafish) gusb XP_700122.6
Drosophila melanogaster (fruit fly) CG15117 NP_725851.1
Caenorhabditis elegans CELE_Y105E8B.9 NP_493548.1
Xenopus (Silurana) tropicalis (western clawed frog) gusb XP_002935849.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GUSB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GUSB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu41505 XM_005250297 PREDICTED: Homo sapiens glucuronidase, beta (GUSB), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu58165 XM_011516113 PREDICTED: Homo sapiens glucuronidase, beta (GUSB), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu58166 XM_011516114 PREDICTED: Homo sapiens glucuronidase, beta (GUSB), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu27736 NM_001284290 Homo sapiens glucuronidase, beta (GUSB), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu27738 NM_001293104 Homo sapiens glucuronidase, beta (GUSB), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu27739 NM_001293105 Homo sapiens glucuronidase, beta (GUSB), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu27733
NM_000181 Homo sapiens glucuronidase, beta (GUSB), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu41505
Clone ID Related Accession (Same CDS sequence) XM_005250297
Accession Version XM_005250297.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1803bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product beta-glucuronidase isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007933.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578814172. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGCCCGGG GGTCGGCGGT TGCCTGGGCG GCGCTCGGGC CGTTGTTGTG GGGCTGCGCG 
CTGGGGCTGC AGGGCGGGAT GCTGTACCCC CAGGAGAGCC CGTCGCGGGA GTGCAAGGAG
CTGGACGGCC TCTGGAGCTT CCGCGCCGAC TTCTCTGACA ACCGACGCCG GGGCTTCGAG
GAGCAGTGGT ACCGGCGGCC GCTGTGGGAG TCAGGCCCCA CCGTGGACAT GCCAGTTCCC
TCCAGCTTCA ATGACATCAG CCAGGACTGG CGTCTGCGGC ATTTTGTCGG CTGGGTGTGG
TACGAACGGG AGGTGATCCT GCCGGAGCGA TGGACCCAGG ACCTGCGCAC AAGAGTGGTG
CTGAGGATTG GCAGTGCCCA TTCCTATGCC ATCGTGTGGG TGAATGGGGT CGACACGCTA
GAGCATGAGG GGGGCTACCT CCCCTTCGAG GCCGACATCA GCAACCTGGT CCAGGTGGGG
CCCCTGCCCT CCCGGCTCCG AATCACTATC GCCATCAACA ACACACTCAC CCCCACCACC
CTGCCACCAG GGACCATCCA ATACCTGACT GACACCTCCA AGTATCCCAA GGGTTACTTT
GTCCAGAACA CATATTTTGA CTTTTTCAAC TACGCTGGAC TGCAGCGGTC TGTACTTCTG
TACACGACAC CCACCACCTA CATCGATGAC ATCACCGTCA CCACCAGCGT GGAGCAAGAC
AGTGGGCTGG TGAATTACCA GATCTCTGTC AAGGGCAGTA ACCTGTTCAA GTTGGAAGTG
CGTCTTTTGG ATGCAGAAAA CAAAGTCGTG GCGAATGGGA CTGGGACCCA GGGCCAACTT
AAGGTGCCAG GTGTCAGCCT CTGGTGGCCG TACCTGATGC ACGAACGCCC TGCCTATCTG
TATTCATTGG AGATCCGAGG GAAGGGCTTC GACTGGCCGC TGCTGGTGAA GGACTTCAAC
CTGCTTCGCT GGCTTGGTGC CAACGCTTTC CGTACCAGCC ACTACCCCTA TGCAGAGGAA
GTGATGCAGA TGTGTGACCG CTATGGGATT GTGGTCATCG ATGAGTGTCC CGGCGTGGGC
CTGGCGCTGC CGCAGTTCTT CAACAACGTT TCTCTGCATC ACCACATGCA GGTGATGGAA
GAAGTGGTGC GTAGGGACAA GAACCACCCC GCGGTCGTGA TGTGGTCTGT GGCCAACGAG
CCTGCGTCCC ACCTAGAATC TGCTGGCTAC TACTTGAAGA TGGTGATCGC TCACACCAAA
TCCTTGGACC CCTCCCGGCC TGTGACCTTT GTGAGCAACT CTAACTATGC AGCAGACAAG
GGGGCTCCGT ATGTGGATGT GATCTGTTTG AACAGCTACT ACTCTTGGTA TCACGACTAC
GGGCACCTGG AGTTGATTCA GCTGCAGCTG GCCACCCAGT TTGAGAACTG GTATAAGAAG
TATCAGAAGC CCATTATTCA GAGCGAGTAT GGAGCAGAAA CGATTGCAGG GTTTCACCAG
GATCCACCTC TGATGTTCAC TGAAGAGTAC CAGAAAAGTC TGCTAGAGCA GTACCATCTG
GGTCTGGATC AAAAACGCAG AAAATACGTG GTTGGAGAGC TCATTTGGAA TTTTGCCGAT
TTCATGACTG AACAGTCACC GACGAGAGTG CTGGGGAATA AAAAGGGGAT CTTCACTCGG
CAGAGACAAC CAAAAAGTGC AGCGTTCCTT TTGCGAGAGA GATACTGGAA GATTGCCAAT
GAAACCAGGT ATCCCCACTC AGTAGCCAAG TCACAATGTT TGGAAAACAG CCTGTTTACT
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005250354.1
CDS127..1929
Misc Feature(1)205..1866(+)
Misc Feature(2)229..798(+)
Misc Feature(3)802..1044(+)
Misc Feature(4)1039..1866(+)
Translation

Target ORF information:

RefSeq Version XM_005250297
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens glucuronidase, beta (GUSB), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005250297

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGCCCGGG GGTCGGCGGT TGCCTGGGCG GCGCTCGGGC CGTTGTTGTG GGGCTGCGCG 
CTGGGGCTGC AGGGCGGGAT GCTGTACCCC CAGGAGAGCC CGTCGCGGGA GTGCAAGGAG
CTGGACGGCC TCTGGAGCTT CCGCGCCGAC TTCTCTGACA ACCGACGCCG GGGCTTCGAG
GAGCAGTGGT ACCGGCGGCC GCTGTGGGAG TCAGGCCCCA CCGTGGACAT GCCAGTTCCC
TCCAGCTTCA ATGACATCAG CCAGGACTGG CGTCTGCGGC ATTTTGTCGG CTGGGTGTGG
TACGAACGGG AGGTGATCCT GCCGGAGCGA TGGACCCAGG ACCTGCGCAC AAGAGTGGTG
CTGAGGATTG GCAGTGCCCA TTCCTATGCC ATCGTGTGGG TGAATGGGGT CGACACGCTA
GAGCATGAGG GGGGCTACCT CCCCTTCGAG GCCGACATCA GCAACCTGGT CCAGGTGGGG
CCCCTGCCCT CCCGGCTCCG AATCACTATC GCCATCAACA ACACACTCAC CCCCACCACC
CTGCCACCAG GGACCATCCA ATACCTGACT GACACCTCCA AGTATCCCAA GGGTTACTTT
GTCCAGAACA CATATTTTGA CTTTTTCAAC TACGCTGGAC TGCAGCGGTC TGTACTTCTG
TACACGACAC CCACCACCTA CATCGATGAC ATCACCGTCA CCACCAGCGT GGAGCAAGAC
AGTGGGCTGG TGAATTACCA GATCTCTGTC AAGGGCAGTA ACCTGTTCAA GTTGGAAGTG
CGTCTTTTGG ATGCAGAAAA CAAAGTCGTG GCGAATGGGA CTGGGACCCA GGGCCAACTT
AAGGTGCCAG GTGTCAGCCT CTGGTGGCCG TACCTGATGC ACGAACGCCC TGCCTATCTG
TATTCATTGG AGATCCGAGG GAAGGGCTTC GACTGGCCGC TGCTGGTGAA GGACTTCAAC
CTGCTTCGCT GGCTTGGTGC CAACGCTTTC CGTACCAGCC ACTACCCCTA TGCAGAGGAA
GTGATGCAGA TGTGTGACCG CTATGGGATT GTGGTCATCG ATGAGTGTCC CGGCGTGGGC
CTGGCGCTGC CGCAGTTCTT CAACAACGTT TCTCTGCATC ACCACATGCA GGTGATGGAA
GAAGTGGTGC GTAGGGACAA GAACCACCCC GCGGTCGTGA TGTGGTCTGT GGCCAACGAG
CCTGCGTCCC ACCTAGAATC TGCTGGCTAC TACTTGAAGA TGGTGATCGC TCACACCAAA
TCCTTGGACC CCTCCCGGCC TGTGACCTTT GTGAGCAACT CTAACTATGC AGCAGACAAG
GGGGCTCCGT ATGTGGATGT GATCTGTTTG AACAGCTACT ACTCTTGGTA TCACGACTAC
GGGCACCTGG AGTTGATTCA GCTGCAGCTG GCCACCCAGT TTGAGAACTG GTATAAGAAG
TATCAGAAGC CCATTATTCA GAGCGAGTAT GGAGCAGAAA CGATTGCAGG GTTTCACCAG
GATCCACCTC TGATGTTCAC TGAAGAGTAC CAGAAAAGTC TGCTAGAGCA GTACCATCTG
GGTCTGGATC AAAAACGCAG AAAATACGTG GTTGGAGAGC TCATTTGGAA TTTTGCCGAT
TTCATGACTG AACAGTCACC GACGAGAGTG CTGGGGAATA AAAAGGGGAT CTTCACTCGG
CAGAGACAAC CAAAAAGTGC AGCGTTCCTT TTGCGAGAGA GATACTGGAA GATTGCCAAT
GAAACCAGGT ATCCCCACTC AGTAGCCAAG TCACAATGTT TGGAAAACAG CCTGTTTACT
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu58165
Clone ID Related Accession (Same CDS sequence) XM_011516113
Accession Version XM_011516113.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1455bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product beta-glucuronidase isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007933.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGACCCAG GACCTGCGCA CAAGAGTGGT GCTGAGGATT GGCAGTGCCC ATTCCTATGC 
CATCGTGGTA TCCCAAGGGT TACTTTGTCC AGAACACATA TTTTGACTTT TTCAACTACG
CTGGACTGCA GCGGTCTGTA CTTCTGTACA CGACACCCAC CACCTACATC GATGACATCA
CCGTCACCAC CAGCGTGGAG CAAGACAGTG GTGAGGGCTT CTGGGCTGGT GAATTACCAG
ATCTCTGTCA AGGGCAGTAA CCTGTTCAAG TTGGAAGTGC GTCTTTTGGA TGCAGAAAAC
AAAGTCGTGG CGAATGGGAC TGGGACCCAG GGCCAACTTA AGGTGCCAGG TGTCAGCCTC
TGGTGGCCGT ACCTGATGCA CGAACGCCCT GCCTATCTGT ATTCATTGGA GGTGCAGCTG
ACTGCACAGA CGTCACTGGG GCCTGTGTCT GACTTCTACA CACTCCCTGT GGGGATCCGC
ACTGTGGCTG TCACCAAGAG CCAGTTCCTC ATCAATGGGA AACCTTTCTA TTTCCACGGT
GTCAACAAGC ATGAGGATGC GGACATCCGA GGGAAGGGCT TCGACTGGCC GCTGCTGGTG
AAGGACTTCA ACCTGCTTCG CTGGCTTGGT GCCAACGCTT TCCGTACCAG CCACTACCCC
TATGCAGAGG AAGTGATGCA GATGTGTGAC CGCTATGGGA TTGTGGTCAT CGATGAGTGT
CCCGGCGTGG GCCTGGCGCT GCCGCAGTTC TTCAACAACG TTTCTCTGCA TCACCACATG
CAGGTGATGG AAGAAGTGGT GCGTAGGGAC AAGAACCACC CCGCGGTCGT GATGTGGTCT
GTGGCCAACG AGCCTGCGTC CCACCTAGAA TCTGCTGGCT ACTACTTGAA GATGGTGATC
GCTCACACCA AATCCTTGGA CCCCTCCCGG CCTGTGACCT TTGTGAGCAA CTCTAACTAT
GCAGCAGACA AGGGGGCTCC GTATGTGGAT GTGATCTGTT TGAACAGCTA CTACTCTTGG
TATCACGACT ACGGGCACCT GGAGTTGATT CAGCTGCAGC TGGCCACCCA GTTTGAGAAC
TGGTATAAGA AGTATCAGAA GCCCATTATT CAGAGCGAGT ATGGAGCAGA AACGATTGCA
GGGTTTCACC AGGATCCACC TCTGATGTTC ACTGAAGAGT ACCAGAAAAG TCTGCTAGAG
CAGTACCATC TGGGTCTGGA TCAAAAACGC AGAAAATACG TGGTTGGAGA GCTCATTTGG
AATTTTGCCG ATTTCATGAC TGAACAGTCA CCGACGAGAG TGCTGGGGAA TAAAAAGGGG
ATCTTCACTC GGCAGAGACA ACCAAAAAGT GCAGCGTTCC TTTTGCGAGA GAGATACTGG
AAGATTGCCA ATGAAACCAG GTATCCCCAC TCAGTAGCCA AGTCACAATG TTTGGAAAAC
AGCCTGTTTA CTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011514415.1
CDS377..1831
Misc Feature(1)860..1768(+)
Translation

Target ORF information:

RefSeq Version XM_011516113
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens glucuronidase, beta (GUSB), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011516113

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGACCCAG GACCTGCGCA CAAGAGTGGT GCTGAGGATT GGCAGTGCCC ATTCCTATGC 
CATCGTGGTA TCCCAAGGGT TACTTTGTCC AGAACACATA TTTTGACTTT TTCAACTACG
CTGGACTGCA GCGGTCTGTA CTTCTGTACA CGACACCCAC CACCTACATC GATGACATCA
CCGTCACCAC CAGCGTGGAG CAAGACAGTG GTGAGGGCTT CTGGGCTGGT GAATTACCAG
ATCTCTGTCA AGGGCAGTAA CCTGTTCAAG TTGGAAGTGC GTCTTTTGGA TGCAGAAAAC
AAAGTCGTGG CGAATGGGAC TGGGACCCAG GGCCAACTTA AGGTGCCAGG TGTCAGCCTC
TGGTGGCCGT ACCTGATGCA CGAACGCCCT GCCTATCTGT ATTCATTGGA GGTGCAGCTG
ACTGCACAGA CGTCACTGGG GCCTGTGTCT GACTTCTACA CACTCCCTGT GGGGATCCGC
ACTGTGGCTG TCACCAAGAG CCAGTTCCTC ATCAATGGGA AACCTTTCTA TTTCCACGGT
GTCAACAAGC ATGAGGATGC GGACATCCGA GGGAAGGGCT TCGACTGGCC GCTGCTGGTG
AAGGACTTCA ACCTGCTTCG CTGGCTTGGT GCCAACGCTT TCCGTACCAG CCACTACCCC
TATGCAGAGG AAGTGATGCA GATGTGTGAC CGCTATGGGA TTGTGGTCAT CGATGAGTGT
CCCGGCGTGG GCCTGGCGCT GCCGCAGTTC TTCAACAACG TTTCTCTGCA TCACCACATG
CAGGTGATGG AAGAAGTGGT GCGTAGGGAC AAGAACCACC CCGCGGTCGT GATGTGGTCT
GTGGCCAACG AGCCTGCGTC CCACCTAGAA TCTGCTGGCT ACTACTTGAA GATGGTGATC
GCTCACACCA AATCCTTGGA CCCCTCCCGG CCTGTGACCT TTGTGAGCAA CTCTAACTAT
GCAGCAGACA AGGGGGCTCC GTATGTGGAT GTGATCTGTT TGAACAGCTA CTACTCTTGG
TATCACGACT ACGGGCACCT GGAGTTGATT CAGCTGCAGC TGGCCACCCA GTTTGAGAAC
TGGTATAAGA AGTATCAGAA GCCCATTATT CAGAGCGAGT ATGGAGCAGA AACGATTGCA
GGGTTTCACC AGGATCCACC TCTGATGTTC ACTGAAGAGT ACCAGAAAAG TCTGCTAGAG
CAGTACCATC TGGGTCTGGA TCAAAAACGC AGAAAATACG TGGTTGGAGA GCTCATTTGG
AATTTTGCCG ATTTCATGAC TGAACAGTCA CCGACGAGAG TGCTGGGGAA TAAAAAGGGG
ATCTTCACTC GGCAGAGACA ACCAAAAAGT GCAGCGTTCC TTTTGCGAGA GAGATACTGG
AAGATTGCCA ATGAAACCAG GTATCCCCAC TCAGTAGCCA AGTCACAATG TTTGGAAAAC
AGCCTGTTTA CTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu58166
Clone ID Related Accession (Same CDS sequence) XM_011516114
Accession Version XM_011516114.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1284bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product beta-glucuronidase isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007933.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGACATCAC CGTCACCACC AGCGTGGAGC AAGACAGTGG TGAGGGCTTC TGGGCTGGTG 
AATTACCAGA TCTCTGTCAA GGGCAGTAAC CTGTTCAAGT TGGAAGTGCG TCTTTTGGAT
GCAGAAAACA AAGTCGTGGC GAATGGGACT GGGACCCAGG GCCAACTTAA GGTGCCAGGT
GTCAGCCTCT GGTGGCCGTA CCTGATGCAC GAACGCCCTG CCTATCTGTA TTCATTGGAG
GTGCAGCTGA CTGCACAGAC GTCACTGGGG CCTGTGTCTG ACTTCTACAC ACTCCCTGTG
GGGATCCGCA CTGTGGCTGT CACCAAGAGC CAGTTCCTCA TCAATGGGAA ACCTTTCTAT
TTCCACGGTG TCAACAAGCA TGAGGATGCG GACATCCGAG GGAAGGGCTT CGACTGGCCG
CTGCTGGTGA AGGACTTCAA CCTGCTTCGC TGGCTTGGTG CCAACGCTTT CCGTACCAGC
CACTACCCCT ATGCAGAGGA AGTGATGCAG ATGTGTGACC GCTATGGGAT TGTGGTCATC
GATGAGTGTC CCGGCGTGGG CCTGGCGCTG CCGCAGTTCT TCAACAACGT TTCTCTGCAT
CACCACATGC AGGTGATGGA AGAAGTGGTG CGTAGGGACA AGAACCACCC CGCGGTCGTG
ATGTGGTCTG TGGCCAACGA GCCTGCGTCC CACCTAGAAT CTGCTGGCTA CTACTTGAAG
ATGGTGATCG CTCACACCAA ATCCTTGGAC CCCTCCCGGC CTGTGACCTT TGTGAGCAAC
TCTAACTATG CAGCAGACAA GGGGGCTCCG TATGTGGATG TGATCTGTTT GAACAGCTAC
TACTCTTGGT ATCACGACTA CGGGCACCTG GAGTTGATTC AGCTGCAGCT GGCCACCCAG
TTTGAGAACT GGTATAAGAA GTATCAGAAG CCCATTATTC AGAGCGAGTA TGGAGCAGAA
ACGATTGCAG GGTTTCACCA GGATCCACCT CTGATGTTCA CTGAAGAGTA CCAGAAAAGT
CTGCTAGAGC AGTACCATCT GGGTCTGGAT CAAAAACGCA GAAAATACGT GGTTGGAGAG
CTCATTTGGA ATTTTGCCGA TTTCATGACT GAACAGTCAC CGACGAGAGT GCTGGGGAAT
AAAAAGGGGA TCTTCACTCG GCAGAGACAA CCAAAAAGTG CAGCGTTCCT TTTGCGAGAG
AGATACTGGA AGATTGCCAA TGAAACCAGG TATCCCCACT CAGTAGCCAA GTCACAATGT
TTGGAAAACA GCCTGTTTAC TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011514416.1
CDS476..1759
Misc Feature(1)788..1696(+)
Translation

Target ORF information:

RefSeq Version XM_011516114
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens glucuronidase, beta (GUSB), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011516114

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGACATCAC CGTCACCACC AGCGTGGAGC AAGACAGTGG TGAGGGCTTC TGGGCTGGTG 
AATTACCAGA TCTCTGTCAA GGGCAGTAAC CTGTTCAAGT TGGAAGTGCG TCTTTTGGAT
GCAGAAAACA AAGTCGTGGC GAATGGGACT GGGACCCAGG GCCAACTTAA GGTGCCAGGT
GTCAGCCTCT GGTGGCCGTA CCTGATGCAC GAACGCCCTG CCTATCTGTA TTCATTGGAG
GTGCAGCTGA CTGCACAGAC GTCACTGGGG CCTGTGTCTG ACTTCTACAC ACTCCCTGTG
GGGATCCGCA CTGTGGCTGT CACCAAGAGC CAGTTCCTCA TCAATGGGAA ACCTTTCTAT
TTCCACGGTG TCAACAAGCA TGAGGATGCG GACATCCGAG GGAAGGGCTT CGACTGGCCG
CTGCTGGTGA AGGACTTCAA CCTGCTTCGC TGGCTTGGTG CCAACGCTTT CCGTACCAGC
CACTACCCCT ATGCAGAGGA AGTGATGCAG ATGTGTGACC GCTATGGGAT TGTGGTCATC
GATGAGTGTC CCGGCGTGGG CCTGGCGCTG CCGCAGTTCT TCAACAACGT TTCTCTGCAT
CACCACATGC AGGTGATGGA AGAAGTGGTG CGTAGGGACA AGAACCACCC CGCGGTCGTG
ATGTGGTCTG TGGCCAACGA GCCTGCGTCC CACCTAGAAT CTGCTGGCTA CTACTTGAAG
ATGGTGATCG CTCACACCAA ATCCTTGGAC CCCTCCCGGC CTGTGACCTT TGTGAGCAAC
TCTAACTATG CAGCAGACAA GGGGGCTCCG TATGTGGATG TGATCTGTTT GAACAGCTAC
TACTCTTGGT ATCACGACTA CGGGCACCTG GAGTTGATTC AGCTGCAGCT GGCCACCCAG
TTTGAGAACT GGTATAAGAA GTATCAGAAG CCCATTATTC AGAGCGAGTA TGGAGCAGAA
ACGATTGCAG GGTTTCACCA GGATCCACCT CTGATGTTCA CTGAAGAGTA CCAGAAAAGT
CTGCTAGAGC AGTACCATCT GGGTCTGGAT CAAAAACGCA GAAAATACGT GGTTGGAGAG
CTCATTTGGA ATTTTGCCGA TTTCATGACT GAACAGTCAC CGACGAGAGT GCTGGGGAAT
AAAAAGGGGA TCTTCACTCG GCAGAGACAA CCAAAAAGTG CAGCGTTCCT TTTGCGAGAG
AGATACTGGA AGATTGCCAA TGAAACCAGG TATCCCCACT CAGTAGCCAA GTCACAATGT
TTGGAAAACA GCCTGTTTAC TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27736
Clone ID Related Accession (Same CDS sequence) NM_001284290
Accession Version NM_001284290.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1518bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product beta-glucuronidase isoform 2 precursor
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC346632.1, AK303819.1, AC073261.8 and AA947121.1. On Sep 27, 2013 this sequence version replaced gi:530385801. Transcript Variant: This variant (2) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 2, which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK303819.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2162568 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCCCGGG GGTCGGCGGT TGCCTGGGCG GCGCTCGGGC CGTTGTTGTG GGGCTGCGCG 
CTGGGGCTGC AGGGCGGGAT GCTGTACCCC CAGGAGAGCC CGTCGCGGGA GTGCAAGGAG
CTGGACGGCC TCTGGAGCTT CCGCGCCGAC TTCTCTGACA ACCGACGCCG GGGCTTCGAG
GAGCAGTGGT ACCGGCGGCC GCTGTGGGAG TCAGGCCCCA CCGTGGACAT GCCAGTTCCC
TCCAGCTTCA ATGACATCAG CCAGGACTGG CGTCTGCGGC ATTTTGTCGG CTGGGTGTGG
TACGAACGGG AGGTGATCCT GCCGGAGCGA TGGACCCAGG ACCTGCGCAC AAGAGTGGTG
CTGAGGATTG GCAGTGCCCA TTCCTATGCC ATCGTGTGGG TGAATGGGGT CGACACGCTA
GAGCATGAGG GGGGCTACCT CCCCTTCGAG GCCGACATCA GCAACCTGGT CCAGGTGCAG
CTGACTGCAC AGACGTCACT GGGGCCTGTG TCTGACTTCT ACACACTCCC TGTGGGGATC
CGCACTGTGG CTGTCACCAA GAGCCAGTTC CTCATCAATG GGAAACCTTT CTATTTCCAC
GGTGTCAACA AGCATGAGGA TGCGGACATC CGAGGGAAGG GCTTCGACTG GCCGCTGCTG
GTGAAGGACT TCAACCTGCT TCGCTGGCTT GGTGCCAACG CTTTCCGTAC CAGCCACTAC
CCCTATGCAG AGGAAGTGAT GCAGATGTGT GACCGCTATG GGATTGTGGT CATCGATGAG
TGTCCCGGCG TGGGCCTGGC GCTGCCGCAG TTCTTCAACA ACGTTTCTCT GCATCACCAC
ATGCAGGTGA TGGAAGAAGT GGTGCGTAGG GACAAGAACC ACCCCGCGGT CGTGATGTGG
TCTGTGGCCA ACGAGCCTGC GTCCCACCTA GAATCTGCTG GCTACTACTT GAAGATGGTG
ATCGCTCACA CCAAATCCTT GGACCCCTCC CGGCCTGTGA CCTTTGTGAG CAACTCTAAC
TATGCAGCAG ACAAGGGGGC TCCGTATGTG GATGTGATCT GTTTGAACAG CTACTACTCT
TGGTATCACG ACTACGGGCA CCTGGAGTTG ATTCAGCTGC AGCTGGCCAC CCAGTTTGAG
AACTGGTATA AGAAGTATCA GAAGCCCATT ATTCAGAGCG AGTATGGAGC AGAAACGATT
GCAGGGTTTC ACCAGGATCC ACCTCTGATG TTCACTGAAG AGTACCAGAA AAGTCTGCTA
GAGCAGTACC ATCTGGGTCT GGATCAAAAA CGCAGAAAAT ACGTGGTTGG AGAGCTCATT
TGGAATTTTG CCGATTTCAT GACTGAACAG TCACCGACGA GAGTGCTGGG GAATAAAAAG
GGGATCTTCA CTCGGCAGAG ACAACCAAAA AGTGCAGCGT TCCTTTTGCG AGAGAGATAC
TGGAAGATTG CCAATGAAAC CAGGTATCCC CACTCAGTAG CCAAGTCACA ATGTTTGGAA
AACAGCCTGT TTACTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001271219.1
CDS132..1649
Misc Feature(1)93..95(+)
Misc Feature(2)210..1586(+)
Misc Feature(3)234..>605(+)
Misc Feature(4)678..1586(+)
Exon (1)1..341
Gene:GUSB
Gene Synonym:
Exon (2)342..527
Gene:GUSB
Gene Synonym:
Exon (3)528..605
Gene:GUSB
Gene Synonym:
Exon (4)606..758
Gene:GUSB
Gene Synonym:
Exon (5)759..937
Gene:GUSB
Gene Synonym:
Exon (6)938..1084
Gene:GUSB
Gene Synonym:
Exon (7)1085..1169
Gene:GUSB
Gene Synonym:
Exon (8)1170..1346
Gene:GUSB
Gene Synonym:
Exon (9)1347..1482
Gene:GUSB
Gene Synonym:
Exon (10)1483..1862
Gene:GUSB
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001284290
Organism Homo sapiens (human)
Definition Homo sapiens glucuronidase, beta (GUSB), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001284290

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCCCGGG GGTCGGCGGT TGCCTGGGCG GCGCTCGGGC CGTTGTTGTG GGGCTGCGCG 
CTGGGGCTGC AGGGCGGGAT GCTGTACCCC CAGGAGAGCC CGTCGCGGGA GTGCAAGGAG
CTGGACGGCC TCTGGAGCTT CCGCGCCGAC TTCTCTGACA ACCGACGCCG GGGCTTCGAG
GAGCAGTGGT ACCGGCGGCC GCTGTGGGAG TCAGGCCCCA CCGTGGACAT GCCAGTTCCC
TCCAGCTTCA ATGACATCAG CCAGGACTGG CGTCTGCGGC ATTTTGTCGG CTGGGTGTGG
TACGAACGGG AGGTGATCCT GCCGGAGCGA TGGACCCAGG ACCTGCGCAC AAGAGTGGTG
CTGAGGATTG GCAGTGCCCA TTCCTATGCC ATCGTGTGGG TGAATGGGGT CGACACGCTA
GAGCATGAGG GGGGCTACCT CCCCTTCGAG GCCGACATCA GCAACCTGGT CCAGGTGCAG
CTGACTGCAC AGACGTCACT GGGGCCTGTG TCTGACTTCT ACACACTCCC TGTGGGGATC
CGCACTGTGG CTGTCACCAA GAGCCAGTTC CTCATCAATG GGAAACCTTT CTATTTCCAC
GGTGTCAACA AGCATGAGGA TGCGGACATC CGAGGGAAGG GCTTCGACTG GCCGCTGCTG
GTGAAGGACT TCAACCTGCT TCGCTGGCTT GGTGCCAACG CTTTCCGTAC CAGCCACTAC
CCCTATGCAG AGGAAGTGAT GCAGATGTGT GACCGCTATG GGATTGTGGT CATCGATGAG
TGTCCCGGCG TGGGCCTGGC GCTGCCGCAG TTCTTCAACA ACGTTTCTCT GCATCACCAC
ATGCAGGTGA TGGAAGAAGT GGTGCGTAGG GACAAGAACC ACCCCGCGGT CGTGATGTGG
TCTGTGGCCA ACGAGCCTGC GTCCCACCTA GAATCTGCTG GCTACTACTT GAAGATGGTG
ATCGCTCACA CCAAATCCTT GGACCCCTCC CGGCCTGTGA CCTTTGTGAG CAACTCTAAC
TATGCAGCAG ACAAGGGGGC TCCGTATGTG GATGTGATCT GTTTGAACAG CTACTACTCT
TGGTATCACG ACTACGGGCA CCTGGAGTTG ATTCAGCTGC AGCTGGCCAC CCAGTTTGAG
AACTGGTATA AGAAGTATCA GAAGCCCATT ATTCAGAGCG AGTATGGAGC AGAAACGATT
GCAGGGTTTC ACCAGGATCC ACCTCTGATG TTCACTGAAG AGTACCAGAA AAGTCTGCTA
GAGCAGTACC ATCTGGGTCT GGATCAAAAA CGCAGAAAAT ACGTGGTTGG AGAGCTCATT
TGGAATTTTG CCGATTTCAT GACTGAACAG TCACCGACGA GAGTGCTGGG GAATAAAAAG
GGGATCTTCA CTCGGCAGAG ACAACCAAAA AGTGCAGCGT TCCTTTTGCG AGAGAGATAC
TGGAAGATTG CCAATGAAAC CAGGTATCCC CACTCAGTAG CCAAGTCACA ATGTTTGGAA
AACAGCCTGT TTACTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27738
Clone ID Related Accession (Same CDS sequence) NM_001293104
Accession Version NM_001293104.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1386bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product beta-glucuronidase isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC346632.1, CD014092.1, BC014142.2 and AA947121.1. Transcript Variant: This variant (3) lacks an alternate exon in the 5' coding region, compared to variant 1. The encoded protein (isoform 3) is shorter, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CD014092.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCCATCGT GGTATCCCAA GGGTTACTTT GTCCAGAACA CATATTTTGA CTTTTTCAAC 
TACGCTGGAC TGCAGCGGTC TGTACTTCTG TACACGACAC CCACCACCTA CATCGATGAC
ATCACCGTCA CCACCAGCGT GGAGCAAGAC AGTGGGCTGG TGAATTACCA GATCTCTGTC
AAGGGCAGTA ACCTGTTCAA GTTGGAAGTG CGTCTTTTGG ATGCAGAAAA CAAAGTCGTG
GCGAATGGGA CTGGGACCCA GGGCCAACTT AAGGTGCCAG GTGTCAGCCT CTGGTGGCCG
TACCTGATGC ACGAACGCCC TGCCTATCTG TATTCATTGG AGGTGCAGCT GACTGCACAG
ACGTCACTGG GGCCTGTGTC TGACTTCTAC ACACTCCCTG TGGGGATCCG CACTGTGGCT
GTCACCAAGA GCCAGTTCCT CATCAATGGG AAACCTTTCT ATTTCCACGG TGTCAACAAG
CATGAGGATG CGGACATCCG AGGGAAGGGC TTCGACTGGC CGCTGCTGGT GAAGGACTTC
AACCTGCTTC GCTGGCTTGG TGCCAACGCT TTCCGTACCA GCCACTACCC CTATGCAGAG
GAAGTGATGC AGATGTGTGA CCGCTATGGG ATTGTGGTCA TCGATGAGTG TCCCGGCGTG
GGCCTGGCGC TGCCGCAGTT CTTCAACAAC GTTTCTCTGC ATCACCACAT GCAGGTGATG
GAAGAAGTGG TGCGTAGGGA CAAGAACCAC CCCGCGGTCG TGATGTGGTC TGTGGCCAAC
GAGCCTGCGT CCCACCTAGA ATCTGCTGGC TACTACTTGA AGATGGTGAT CGCTCACACC
AAATCCTTGG ACCCCTCCCG GCCTGTGACC TTTGTGAGCA ACTCTAACTA TGCAGCAGAC
AAGGGGGCTC CGTATGTGGA TGTGATCTGT TTGAACAGCT ACTACTCTTG GTATCACGAC
TACGGGCACC TGGAGTTGAT TCAGCTGCAG CTGGCCACCC AGTTTGAGAA CTGGTATAAG
AAGTATCAGA AGCCCATTAT TCAGAGCGAG TATGGAGCAG AAACGATTGC AGGGTTTCAC
CAGGATCCAC CTCTGATGTT CACTGAAGAG TACCAGAAAA GTCTGCTAGA GCAGTACCAT
CTGGGTCTGG ATCAAAAACG CAGAAAATAC GTGGTTGGAG AGCTCATTTG GAATTTTGCC
GATTTCATGA CTGAACAGTC ACCGACGAGA GTGCTGGGGA ATAAAAAGGG GATCTTCACT
CGGCAGAGAC AACCAAAAAG TGCAGCGTTC CTTTTGCGAG AGAGATACTG GAAGATTGCC
AATGAAACCA GGTATCCCCA CTCAGTAGCC AAGTCACAAT GTTTGGAAAA CAGCCTGTTT
ACTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001280033.1
CDS517..1902
Misc Feature(1)493..495(+)
Misc Feature(2)622..927(+)
Misc Feature(3)931..1839(+)
Exon (1)1..341
Gene:GUSB
Gene Synonym:
Exon (2)342..527
Gene:GUSB
Gene Synonym:
Exon (3)528..670
Gene:GUSB
Gene Synonym:
Exon (4)671..858
Gene:GUSB
Gene Synonym:
Exon (5)859..1011
Gene:GUSB
Gene Synonym:
Exon (6)1012..1190
Gene:GUSB
Gene Synonym:
Exon (7)1191..1337
Gene:GUSB
Gene Synonym:
Exon (8)1338..1422
Gene:GUSB
Gene Synonym:
Exon (9)1423..1599
Gene:GUSB
Gene Synonym:
Exon (10)1600..1735
Gene:GUSB
Gene Synonym:
Exon (11)1736..2115
Gene:GUSB
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001293104
Organism Homo sapiens (human)
Definition Homo sapiens glucuronidase, beta (GUSB), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001293104

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCCATCGT GGTATCCCAA GGGTTACTTT GTCCAGAACA CATATTTTGA CTTTTTCAAC 
TACGCTGGAC TGCAGCGGTC TGTACTTCTG TACACGACAC CCACCACCTA CATCGATGAC
ATCACCGTCA CCACCAGCGT GGAGCAAGAC AGTGGGCTGG TGAATTACCA GATCTCTGTC
AAGGGCAGTA ACCTGTTCAA GTTGGAAGTG CGTCTTTTGG ATGCAGAAAA CAAAGTCGTG
GCGAATGGGA CTGGGACCCA GGGCCAACTT AAGGTGCCAG GTGTCAGCCT CTGGTGGCCG
TACCTGATGC ACGAACGCCC TGCCTATCTG TATTCATTGG AGGTGCAGCT GACTGCACAG
ACGTCACTGG GGCCTGTGTC TGACTTCTAC ACACTCCCTG TGGGGATCCG CACTGTGGCT
GTCACCAAGA GCCAGTTCCT CATCAATGGG AAACCTTTCT ATTTCCACGG TGTCAACAAG
CATGAGGATG CGGACATCCG AGGGAAGGGC TTCGACTGGC CGCTGCTGGT GAAGGACTTC
AACCTGCTTC GCTGGCTTGG TGCCAACGCT TTCCGTACCA GCCACTACCC CTATGCAGAG
GAAGTGATGC AGATGTGTGA CCGCTATGGG ATTGTGGTCA TCGATGAGTG TCCCGGCGTG
GGCCTGGCGC TGCCGCAGTT CTTCAACAAC GTTTCTCTGC ATCACCACAT GCAGGTGATG
GAAGAAGTGG TGCGTAGGGA CAAGAACCAC CCCGCGGTCG TGATGTGGTC TGTGGCCAAC
GAGCCTGCGT CCCACCTAGA ATCTGCTGGC TACTACTTGA AGATGGTGAT CGCTCACACC
AAATCCTTGG ACCCCTCCCG GCCTGTGACC TTTGTGAGCA ACTCTAACTA TGCAGCAGAC
AAGGGGGCTC CGTATGTGGA TGTGATCTGT TTGAACAGCT ACTACTCTTG GTATCACGAC
TACGGGCACC TGGAGTTGAT TCAGCTGCAG CTGGCCACCC AGTTTGAGAA CTGGTATAAG
AAGTATCAGA AGCCCATTAT TCAGAGCGAG TATGGAGCAG AAACGATTGC AGGGTTTCAC
CAGGATCCAC CTCTGATGTT CACTGAAGAG TACCAGAAAA GTCTGCTAGA GCAGTACCAT
CTGGGTCTGG ATCAAAAACG CAGAAAATAC GTGGTTGGAG AGCTCATTTG GAATTTTGCC
GATTTCATGA CTGAACAGTC ACCGACGAGA GTGCTGGGGA ATAAAAAGGG GATCTTCACT
CGGCAGAGAC AACCAAAAAG TGCAGCGTTC CTTTTGCGAG AGAGATACTG GAAGATTGCC
AATGAAACCA GGTATCCCCA CTCAGTAGCC AAGTCACAAT GTTTGGAAAA CAGCCTGTTT
ACTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27739
Clone ID Related Accession (Same CDS sequence) NM_001293105
Accession Version NM_001293105.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1299bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product beta-glucuronidase isoform 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC346632.1, CD014093.1, BC014142.2 and AA947121.1. Transcript Variant: This variant (4) lacks alternate exons in the 5' coding region, compared to variant 1. The encoded protein (isoform 4) is shorter, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CD014093.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGACCCAG GACCTGCGCA CAAGAGTGGT GCTGAGGATT GGCAGTGCCC ATTCCTATGC 
CATCGTGGGC TGGTGAATTA CCAGATCTCT GTCAAGGGCA GTAACCTGTT CAAGTTGGAA
GTGCGTCTTT TGGATGCAGA AAACAAAGTC GTGGCGAATG GGACTGGGAC CCAGGGCCAA
CTTAAGGTGC CAGGTGTCAG CCTCTGGTGG CCGTACCTGA TGCACGAACG CCCTGCCTAT
CTGTATTCAT TGGAGGTGCA GCTGACTGCA CAGACGTCAC TGGGGCCTGT GTCTGACTTC
TACACACTCC CTGTGGGGAT CCGCACTGTG GCTGTCACCA AGAGCCAGTT CCTCATCAAT
GGGAAACCTT TCTATTTCCA CGGTGTCAAC AAGCATGAGG ATGCGGACAT CCGAGGGAAG
GGCTTCGACT GGCCGCTGCT GGTGAAGGAC TTCAACCTGC TTCGCTGGCT TGGTGCCAAC
GCTTTCCGTA CCAGCCACTA CCCCTATGCA GAGGAAGTGA TGCAGATGTG TGACCGCTAT
GGGATTGTGG TCATCGATGA GTGTCCCGGC GTGGGCCTGG CGCTGCCGCA GTTCTTCAAC
AACGTTTCTC TGCATCACCA CATGCAGGTG ATGGAAGAAG TGGTGCGTAG GGACAAGAAC
CACCCCGCGG TCGTGATGTG GTCTGTGGCC AACGAGCCTG CGTCCCACCT AGAATCTGCT
GGCTACTACT TGAAGATGGT GATCGCTCAC ACCAAATCCT TGGACCCCTC CCGGCCTGTG
ACCTTTGTGA GCAACTCTAA CTATGCAGCA GACAAGGGGG CTCCGTATGT GGATGTGATC
TGTTTGAACA GCTACTACTC TTGGTATCAC GACTACGGGC ACCTGGAGTT GATTCAGCTG
CAGCTGGCCA CCCAGTTTGA GAACTGGTAT AAGAAGTATC AGAAGCCCAT TATTCAGAGC
GAGTATGGAG CAGAAACGAT TGCAGGGTTT CACCAGGATC CACCTCTGAT GTTCACTGAA
GAGTACCAGA AAAGTCTGCT AGAGCAGTAC CATCTGGGTC TGGATCAAAA ACGCAGAAAA
TACGTGGTTG GAGAGCTCAT TTGGAATTTT GCCGATTTCA TGACTGAACA GTCACCGACG
AGAGTGCTGG GGAATAAAAA GGGGATCTTC ACTCGGCAGA GACAACCAAA AAGTGCAGCG
TTCCTTTTGC GAGAGAGATA CTGGAAGATT GCCAATGAAA CCAGGTATCC CCACTCAGTA
GCCAAGTCAC AATGTTTGGA AAACAGCCTG TTTACTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001280034.1
CDS461..1759
Misc Feature(1)383..385(+)
Misc Feature(2)788..1696(+)
Exon (1)1..341
Gene:GUSB
Gene Synonym:
Exon (2)342..527
Gene:GUSB
Gene Synonym:
Exon (3)528..715
Gene:GUSB
Gene Synonym:
Exon (4)716..868
Gene:GUSB
Gene Synonym:
Exon (5)869..1047
Gene:GUSB
Gene Synonym:
Exon (6)1048..1194
Gene:GUSB
Gene Synonym:
Exon (7)1195..1279
Gene:GUSB
Gene Synonym:
Exon (8)1280..1456
Gene:GUSB
Gene Synonym:
Exon (9)1457..1592
Gene:GUSB
Gene Synonym:
Exon (10)1593..1972
Gene:GUSB
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001293105
Organism Homo sapiens (human)
Definition Homo sapiens glucuronidase, beta (GUSB), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001293105

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGACCCAG GACCTGCGCA CAAGAGTGGT GCTGAGGATT GGCAGTGCCC ATTCCTATGC 
CATCGTGGGC TGGTGAATTA CCAGATCTCT GTCAAGGGCA GTAACCTGTT CAAGTTGGAA
GTGCGTCTTT TGGATGCAGA AAACAAAGTC GTGGCGAATG GGACTGGGAC CCAGGGCCAA
CTTAAGGTGC CAGGTGTCAG CCTCTGGTGG CCGTACCTGA TGCACGAACG CCCTGCCTAT
CTGTATTCAT TGGAGGTGCA GCTGACTGCA CAGACGTCAC TGGGGCCTGT GTCTGACTTC
TACACACTCC CTGTGGGGAT CCGCACTGTG GCTGTCACCA AGAGCCAGTT CCTCATCAAT
GGGAAACCTT TCTATTTCCA CGGTGTCAAC AAGCATGAGG ATGCGGACAT CCGAGGGAAG
GGCTTCGACT GGCCGCTGCT GGTGAAGGAC TTCAACCTGC TTCGCTGGCT TGGTGCCAAC
GCTTTCCGTA CCAGCCACTA CCCCTATGCA GAGGAAGTGA TGCAGATGTG TGACCGCTAT
GGGATTGTGG TCATCGATGA GTGTCCCGGC GTGGGCCTGG CGCTGCCGCA GTTCTTCAAC
AACGTTTCTC TGCATCACCA CATGCAGGTG ATGGAAGAAG TGGTGCGTAG GGACAAGAAC
CACCCCGCGG TCGTGATGTG GTCTGTGGCC AACGAGCCTG CGTCCCACCT AGAATCTGCT
GGCTACTACT TGAAGATGGT GATCGCTCAC ACCAAATCCT TGGACCCCTC CCGGCCTGTG
ACCTTTGTGA GCAACTCTAA CTATGCAGCA GACAAGGGGG CTCCGTATGT GGATGTGATC
TGTTTGAACA GCTACTACTC TTGGTATCAC GACTACGGGC ACCTGGAGTT GATTCAGCTG
CAGCTGGCCA CCCAGTTTGA GAACTGGTAT AAGAAGTATC AGAAGCCCAT TATTCAGAGC
GAGTATGGAG CAGAAACGAT TGCAGGGTTT CACCAGGATC CACCTCTGAT GTTCACTGAA
GAGTACCAGA AAAGTCTGCT AGAGCAGTAC CATCTGGGTC TGGATCAAAA ACGCAGAAAA
TACGTGGTTG GAGAGCTCAT TTGGAATTTT GCCGATTTCA TGACTGAACA GTCACCGACG
AGAGTGCTGG GGAATAAAAA GGGGATCTTC ACTCGGCAGA GACAACCAAA AAGTGCAGCG
TTCCTTTTGC GAGAGAGATA CTGGAAGATT GCCAATGAAA CCAGGTATCC CCACTCAGTA
GCCAAGTCAC AATGTTTGGA AAACAGCCTG TTTACTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27733
Clone ID Related Accession (Same CDS sequence) NM_000181
Accession Version NM_000181.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1956bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product beta-glucuronidase isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC346632.1, BC014142.2 and BM719712.1. This sequence is a reference standard in the RefSeqGene project. On Nov 19, 2009 this sequence version replaced gi:119372313. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK223406.1, M15182.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGCCCGGG GGTCGGCGGT TGCCTGGGCG GCGCTCGGGC CGTTGTTGTG GGGCTGCGCG 
CTGGGGCTGC AGGGCGGGAT GCTGTACCCC CAGGAGAGCC CGTCGCGGGA GTGCAAGGAG
CTGGACGGCC TCTGGAGCTT CCGCGCCGAC TTCTCTGACA ACCGACGCCG GGGCTTCGAG
GAGCAGTGGT ACCGGCGGCC GCTGTGGGAG TCAGGCCCCA CCGTGGACAT GCCAGTTCCC
TCCAGCTTCA ATGACATCAG CCAGGACTGG CGTCTGCGGC ATTTTGTCGG CTGGGTGTGG
TACGAACGGG AGGTGATCCT GCCGGAGCGA TGGACCCAGG ACCTGCGCAC AAGAGTGGTG
CTGAGGATTG GCAGTGCCCA TTCCTATGCC ATCGTGTGGG TGAATGGGGT CGACACGCTA
GAGCATGAGG GGGGCTACCT CCCCTTCGAG GCCGACATCA GCAACCTGGT CCAGGTGGGG
CCCCTGCCCT CCCGGCTCCG AATCACTATC GCCATCAACA ACACACTCAC CCCCACCACC
CTGCCACCAG GGACCATCCA ATACCTGACT GACACCTCCA AGTATCCCAA GGGTTACTTT
GTCCAGAACA CATATTTTGA CTTTTTCAAC TACGCTGGAC TGCAGCGGTC TGTACTTCTG
TACACGACAC CCACCACCTA CATCGATGAC ATCACCGTCA CCACCAGCGT GGAGCAAGAC
AGTGGGCTGG TGAATTACCA GATCTCTGTC AAGGGCAGTA ACCTGTTCAA GTTGGAAGTG
CGTCTTTTGG ATGCAGAAAA CAAAGTCGTG GCGAATGGGA CTGGGACCCA GGGCCAACTT
AAGGTGCCAG GTGTCAGCCT CTGGTGGCCG TACCTGATGC ACGAACGCCC TGCCTATCTG
TATTCATTGG AGGTGCAGCT GACTGCACAG ACGTCACTGG GGCCTGTGTC TGACTTCTAC
ACACTCCCTG TGGGGATCCG CACTGTGGCT GTCACCAAGA GCCAGTTCCT CATCAATGGG
AAACCTTTCT ATTTCCACGG TGTCAACAAG CATGAGGATG CGGACATCCG AGGGAAGGGC
TTCGACTGGC CGCTGCTGGT GAAGGACTTC AACCTGCTTC GCTGGCTTGG TGCCAACGCT
TTCCGTACCA GCCACTACCC CTATGCAGAG GAAGTGATGC AGATGTGTGA CCGCTATGGG
ATTGTGGTCA TCGATGAGTG TCCCGGCGTG GGCCTGGCGC TGCCGCAGTT CTTCAACAAC
GTTTCTCTGC ATCACCACAT GCAGGTGATG GAAGAAGTGG TGCGTAGGGA CAAGAACCAC
CCCGCGGTCG TGATGTGGTC TGTGGCCAAC GAGCCTGCGT CCCACCTAGA ATCTGCTGGC
TACTACTTGA AGATGGTGAT CGCTCACACC AAATCCTTGG ACCCCTCCCG GCCTGTGACC
TTTGTGAGCA ACTCTAACTA TGCAGCAGAC AAGGGGGCTC CGTATGTGGA TGTGATCTGT
TTGAACAGCT ACTACTCTTG GTATCACGAC TACGGGCACC TGGAGTTGAT TCAGCTGCAG
CTGGCCACCC AGTTTGAGAA CTGGTATAAG AAGTATCAGA AGCCCATTAT TCAGAGCGAG
TATGGAGCAG AAACGATTGC AGGGTTTCAC CAGGATCCAC CTCTGATGTT CACTGAAGAG
TACCAGAAAA GTCTGCTAGA GCAGTACCAT CTGGGTCTGG ATCAAAAACG CAGAAAATAC
GTGGTTGGAG AGCTCATTTG GAATTTTGCC GATTTCATGA CTGAACAGTC ACCGACGAGA
GTGCTGGGGA ATAAAAAGGG GATCTTCACT CGGCAGAGAC AACCAAAAAG TGCAGCGTTC
CTTTTGCGAG AGAGATACTG GAAGATTGCC AATGAAACCA GGTATCCCCA CTCAGTAGCC
AAGTCACAAT GTTTGGAAAA CAGCCTGTTT ACTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000172.2
CDS132..2087
Misc Feature(1)6..6(+)
Misc Feature(2)89..105(+)
Misc Feature(3)93..95(+)
Misc Feature(4)102..102(+)
Misc Feature(5)210..2024(+)
Misc Feature(6)234..803(+)
Misc Feature(7)807..1112(+)
Misc Feature(8)1116..2024(+)
Exon (1)1..341
Gene:GUSB
Gene Synonym:
Exon (2)342..527
Gene:GUSB
Gene Synonym:
Exon (3)528..712
Gene:GUSB
Gene Synonym:
Exon (4)713..855
Gene:GUSB
Gene Synonym:
Exon (5)856..1043
Gene:GUSB
Gene Synonym:
Exon (6)1044..1196
Gene:GUSB
Gene Synonym:
Exon (7)1197..1375
Gene:GUSB
Gene Synonym:
Exon (8)1376..1522
Gene:GUSB
Gene Synonym:
Exon (9)1523..1607
Gene:GUSB
Gene Synonym:
Exon (10)1608..1784
Gene:GUSB
Gene Synonym:
Exon (11)1785..1920
Gene:GUSB
Gene Synonym:
Exon (12)1921..2300
Gene:GUSB
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000181
Organism Homo sapiens (human)
Definition Homo sapiens glucuronidase, beta (GUSB), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000181

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGCCCGGG GGTCGGCGGT TGCCTGGGCG GCGCTCGGGC CGTTGTTGTG GGGCTGCGCG 
CTGGGGCTGC AGGGCGGGAT GCTGTACCCC CAGGAGAGCC CGTCGCGGGA GTGCAAGGAG
CTGGACGGCC TCTGGAGCTT CCGCGCCGAC TTCTCTGACA ACCGACGCCG GGGCTTCGAG
GAGCAGTGGT ACCGGCGGCC GCTGTGGGAG TCAGGCCCCA CCGTGGACAT GCCAGTTCCC
TCCAGCTTCA ATGACATCAG CCAGGACTGG CGTCTGCGGC ATTTTGTCGG CTGGGTGTGG
TACGAACGGG AGGTGATCCT GCCGGAGCGA TGGACCCAGG ACCTGCGCAC AAGAGTGGTG
CTGAGGATTG GCAGTGCCCA TTCCTATGCC ATCGTGTGGG TGAATGGGGT CGACACGCTA
GAGCATGAGG GGGGCTACCT CCCCTTCGAG GCCGACATCA GCAACCTGGT CCAGGTGGGG
CCCCTGCCCT CCCGGCTCCG AATCACTATC GCCATCAACA ACACACTCAC CCCCACCACC
CTGCCACCAG GGACCATCCA ATACCTGACT GACACCTCCA AGTATCCCAA GGGTTACTTT
GTCCAGAACA CATATTTTGA CTTTTTCAAC TACGCTGGAC TGCAGCGGTC TGTACTTCTG
TACACGACAC CCACCACCTA CATCGATGAC ATCACCGTCA CCACCAGCGT GGAGCAAGAC
AGTGGGCTGG TGAATTACCA GATCTCTGTC AAGGGCAGTA ACCTGTTCAA GTTGGAAGTG
CGTCTTTTGG ATGCAGAAAA CAAAGTCGTG GCGAATGGGA CTGGGACCCA GGGCCAACTT
AAGGTGCCAG GTGTCAGCCT CTGGTGGCCG TACCTGATGC ACGAACGCCC TGCCTATCTG
TATTCATTGG AGGTGCAGCT GACTGCACAG ACGTCACTGG GGCCTGTGTC TGACTTCTAC
ACACTCCCTG TGGGGATCCG CACTGTGGCT GTCACCAAGA GCCAGTTCCT CATCAATGGG
AAACCTTTCT ATTTCCACGG TGTCAACAAG CATGAGGATG CGGACATCCG AGGGAAGGGC
TTCGACTGGC CGCTGCTGGT GAAGGACTTC AACCTGCTTC GCTGGCTTGG TGCCAACGCT
TTCCGTACCA GCCACTACCC CTATGCAGAG GAAGTGATGC AGATGTGTGA CCGCTATGGG
ATTGTGGTCA TCGATGAGTG TCCCGGCGTG GGCCTGGCGC TGCCGCAGTT CTTCAACAAC
GTTTCTCTGC ATCACCACAT GCAGGTGATG GAAGAAGTGG TGCGTAGGGA CAAGAACCAC
CCCGCGGTCG TGATGTGGTC TGTGGCCAAC GAGCCTGCGT CCCACCTAGA ATCTGCTGGC
TACTACTTGA AGATGGTGAT CGCTCACACC AAATCCTTGG ACCCCTCCCG GCCTGTGACC
TTTGTGAGCA ACTCTAACTA TGCAGCAGAC AAGGGGGCTC CGTATGTGGA TGTGATCTGT
TTGAACAGCT ACTACTCTTG GTATCACGAC TACGGGCACC TGGAGTTGAT TCAGCTGCAG
CTGGCCACCC AGTTTGAGAA CTGGTATAAG AAGTATCAGA AGCCCATTAT TCAGAGCGAG
TATGGAGCAG AAACGATTGC AGGGTTTCAC CAGGATCCAC CTCTGATGTT CACTGAAGAG
TACCAGAAAA GTCTGCTAGA GCAGTACCAT CTGGGTCTGG ATCAAAAACG CAGAAAATAC
GTGGTTGGAG AGCTCATTTG GAATTTTGCC GATTTCATGA CTGAACAGTC ACCGACGAGA
GTGCTGGGGA ATAAAAAGGG GATCTTCACT CGGCAGAGAC AACCAAAAAG TGCAGCGTTC
CTTTTGCGAG AGAGATACTG GAAGATTGCC AATGAAACCA GGTATCCCCA CTCAGTAGCC
AAGTCACAAT GTTTGGAAAA CAGCCTGTTT ACTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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