LIMK1 cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following LIMK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LIMK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu16673 NM_001204426.2
Latest version!
Homo sapiens LIM domain kinase 1 (LIMK1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu22318 NM_002314.4
Latest version!
Homo sapiens LIM domain kinase 1 (LIMK1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $384.30
$549.00
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OHu16673 NM_001204426.1 Homo sapiens LIM domain kinase 1 (LIMK1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu22318 NM_002314.3 Homo sapiens LIM domain kinase 1 (LIMK1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $384.30
$549.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu16673
    Clone ID Related Accession (Same CDS sequence) NM_001204426.1 , NM_001204426.2
    Accession Version NM_001204426.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1842bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product LIM domain kinase 1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC296332.1, AK300382.1, D26309.1 and AW016257.1. Transcript Variant: This variant (2) lacks multiple exons at the 5' end and uses a different start codon, compared to variant 1. This variant encodes an isoform (2) with a shorter and distinct N-terminus that lacks one of two LIM zinc-binding domains, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK300382.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCTGTTGG CTTCAGCCCC AAGAAGACGC CGCTTCCTCC AGAGGGCTAA GTGTTGTGAC 
    TGCAGTGCCT CCCTGTCGCA CCAGTACTAT GAGAAGGATG GGCAGCTCTT CTGCAAGAAG
    GACTACTGGG CCCGCTATGG CGAGTCCTGC CATGGGTGCT CTGAGCAAAT CACCAAGGGA
    CTGGTTATGG TGGCTGGGGA GCTGAAGTAC CACCCCGAGT GTTTCATCTG CCTCACGTGT
    GGGACCTTTA TCGGTGACGG GGACACCTAC ACGCTGGTGG AGCACTCCAA GCTGTACTGC
    GGGCACTGCT ACTACCAGAC TGTGGTGACC CCCGTCATCG AGCAGATCCT GCCTGACTCC
    CCTGGCTCCC ACCTGCCCCA CACCGTCACC CTGGTGTCCA TCCCAGCCTC ATCTCATGGC
    AAGCGTGGAC TTTCAGTCTC CATTGACCCC CCGCACGGCC CACCGGGCTG TGGCACCGAG
    CACTCACACA CCGTCCGCGT CCAGGGAGTG GATCCGGGCT GCATGAGCCC AGATGTGAAG
    AATTCCATCC ACGTCGGAGA CCGGATCTTG GAAATCAATG GCACGCCCAT CCGAAATGTG
    CCCCTGGACG AGATTGACCT GCTGATTCAG GAAACCAGCC GCCTGCTCCA GCTGACCCTC
    GAGCATGACC CTCACGATAC ACTGGGCCAC GGGCTGGGGC CTGAGACCAG CCCCCTGAGC
    TCTCCGGCTT ATACTCCCAG CGGGGAGGCG GGCAGCTCTG CCCGGCAGAA ACCTGTCTTG
    AGGAGCTGCA GCATCGACAG GTCTCCGGGC GCTGGCTCAC TGGGCTCCCC GGCCTCCCAG
    CGCAAGGACC TGGGTCGCTC TGAGTCCCTC CGCGTAGTCT GCCGGCCACA CCGCATCTTC
    CGGCCGTCGG ACCTCATCCA CGGGGAGGTG CTGGGCAAGG GCTGCTTCGG CCAGGCTATC
    AAGGTGACAC ACCGTGAGAC AGGTGAGGTG ATGGTGATGA AGGAGCTGAT CCGGTTCGAC
    GAGGAGACCC AGAGGACGTT CCTCAAGGAG GTGAAGGTCA TGCGATGCCT GGAACACCCC
    AACGTGCTCA AGTTCATCGG GGTGCTCTAC AAGGACAAGA GGCTCAACTT CATCACTGAG
    TACATCAAGG GCGGCACGCT CCGGGGCATC ATCAAGAGCA TGGACAGCCA GTACCCATGG
    AGCCAGAGAG TGAGCTTTGC CAAGGACATC GCATCAGGGA TGGCCTACCT CCACTCCATG
    AACATCATCC ACCGAGACCT CAACTCCCAC AACTGCCTGG TCCGCGAGAA CAAGAATGTG
    GTGGTGGCTG ACTTCGGGCT GGCGCGTCTC ATGGTGGACG AGAAGACTCA GCCTGAGGGC
    CTGCGGAGCC TCAAGAAGCC AGACCGCAAG AAGCGCTACA CCGTGGTGGG CAACCCCTAC
    TGGATGGCAC CTGAGATGAT CAACGGCCGC AGCTATGATG AGAAGGTGGA TGTGTTCTCC
    TTTGGGATCG TCCTGTGCGA GATCATCGGG CGGGTGAACG CAGACCCTGA CTACCTGCCC
    CGCACCATGG ACTTTGGCCT CAACGTGCGA GGATTCCTGG ACCGCTACTG CCCCCCAAAC
    TGCCCCCCGA GCTTCTTCCC CATCACCGTG CGCTGTTGCG ATCTGGACCC CGAGAAGAGG
    CCATCCTTTG TGAAGCTGGA ACACTGGCTG GAGACCCTCC GCATGCACCT GGCCGGCCAC
    CTGCCACTGG GCCCACAGCT GGAGCAGCTG GACAGAGGTT TCTGGGAGAC CTACCGGCGC
    GGCGAGAGCG GACTGCCTGC CCACCCTGAG GTCCCCGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001191355.1
    CDS95..1936
    Translation

    Target ORF information:

    RefSeq Version NM_001204426.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM domain kinase 1 (LIMK1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001204426.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCTGTTGG CTTCAGCCCC AAGAAGACGC CGCTTCCTCC AGAGGGCTAA GTGTTGTGAC 
    TGCAGTGCCT CCCTGTCGCA CCAGTACTAT GAGAAGGATG GGCAGCTCTT CTGCAAGAAG
    GACTACTGGG CCCGCTATGG CGAGTCCTGC CATGGGTGCT CTGAGCAAAT CACCAAGGGA
    CTGGTTATGG TGGCTGGGGA GCTGAAGTAC CACCCCGAGT GTTTCATCTG CCTCACGTGT
    GGGACCTTTA TCGGTGACGG GGACACCTAC ACGCTGGTGG AGCACTCCAA GCTGTACTGC
    GGGCACTGCT ACTACCAGAC TGTGGTGACC CCCGTCATCG AGCAGATCCT GCCTGACTCC
    CCTGGCTCCC ACCTGCCCCA CACCGTCACC CTGGTGTCCA TCCCAGCCTC ATCTCATGGC
    AAGCGTGGAC TTTCAGTCTC CATTGACCCC CCGCACGGCC CACCGGGCTG TGGCACCGAG
    CACTCACACA CCGTCCGCGT CCAGGGAGTG GATCCGGGCT GCATGAGCCC AGATGTGAAG
    AATTCCATCC ACGTCGGAGA CCGGATCTTG GAAATCAATG GCACGCCCAT CCGAAATGTG
    CCCCTGGACG AGATTGACCT GCTGATTCAG GAAACCAGCC GCCTGCTCCA GCTGACCCTC
    GAGCATGACC CTCACGATAC ACTGGGCCAC GGGCTGGGGC CTGAGACCAG CCCCCTGAGC
    TCTCCGGCTT ATACTCCCAG CGGGGAGGCG GGCAGCTCTG CCCGGCAGAA ACCTGTCTTG
    AGGAGCTGCA GCATCGACAG GTCTCCGGGC GCTGGCTCAC TGGGCTCCCC GGCCTCCCAG
    CGCAAGGACC TGGGTCGCTC TGAGTCCCTC CGCGTAGTCT GCCGGCCACA CCGCATCTTC
    CGGCCGTCGG ACCTCATCCA CGGGGAGGTG CTGGGCAAGG GCTGCTTCGG CCAGGCTATC
    AAGGTGACAC ACCGTGAGAC AGGTGAGGTG ATGGTGATGA AGGAGCTGAT CCGGTTCGAC
    GAGGAGACCC AGAGGACGTT CCTCAAGGAG GTGAAGGTCA TGCGATGCCT GGAACACCCC
    AACGTGCTCA AGTTCATCGG GGTGCTCTAC AAGGACAAGA GGCTCAACTT CATCACTGAG
    TACATCAAGG GCGGCACGCT CCGGGGCATC ATCAAGAGCA TGGACAGCCA GTACCCATGG
    AGCCAGAGAG TGAGCTTTGC CAAGGACATC GCATCAGGGA TGGCCTACCT CCACTCCATG
    AACATCATCC ACCGAGACCT CAACTCCCAC AACTGCCTGG TCCGCGAGAA CAAGAATGTG
    GTGGTGGCTG ACTTCGGGCT GGCGCGTCTC ATGGTGGACG AGAAGACTCA GCCTGAGGGC
    CTGCGGAGCC TCAAGAAGCC AGACCGCAAG AAGCGCTACA CCGTGGTGGG CAACCCCTAC
    TGGATGGCAC CTGAGATGAT CAACGGCCGC AGCTATGATG AGAAGGTGGA TGTGTTCTCC
    TTTGGGATCG TCCTGTGCGA GATCATCGGG CGGGTGAACG CAGACCCTGA CTACCTGCCC
    CGCACCATGG ACTTTGGCCT CAACGTGCGA GGATTCCTGG ACCGCTACTG CCCCCCAAAC
    TGCCCCCCGA GCTTCTTCCC CATCACCGTG CGCTGTTGCG ATCTGGACCC CGAGAAGAGG
    CCATCCTTTG TGAAGCTGGA ACACTGGCTG GAGACCCTCC GCATGCACCT GGCCGGCCAC
    CTGCCACTGG GCCCACAGCT GGAGCAGCTG GACAGAGGTT TCTGGGAGAC CTACCGGCGC
    GGCGAGAGCG GACTGCCTGC CCACCCTGAG GTCCCCGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16673
    Clone ID Related Accession (Same CDS sequence) NM_001204426.1 , NM_001204426.2
    Accession Version NM_001204426.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1842bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-27
    Organism Homo sapiens(human)
    Product LIM domain kinase 1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK300382.1, D26309.1 and AW016257.1. On May 31, 2019 this sequence version replaced NM_001204426.1. Transcript Variant: This variant (2) lacks multiple exons at the 5' end and uses a different start codon, compared to variant 1. This variant encodes an isoform (2) with a shorter and distinct N-terminus that lacks one of two LIM zinc-binding domains, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK300382.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCTGTTGG CTTCAGCCCC AAGAAGACGC CGCTTCCTCC AGAGGGCTAA GTGTTGTGAC 
    TGCAGTGCCT CCCTGTCGCA CCAGTACTAT GAGAAGGATG GGCAGCTCTT CTGCAAGAAG
    GACTACTGGG CCCGCTATGG CGAGTCCTGC CATGGGTGCT CTGAGCAAAT CACCAAGGGA
    CTGGTTATGG TGGCTGGGGA GCTGAAGTAC CACCCCGAGT GTTTCATCTG CCTCACGTGT
    GGGACCTTTA TCGGTGACGG GGACACCTAC ACGCTGGTGG AGCACTCCAA GCTGTACTGC
    GGGCACTGCT ACTACCAGAC TGTGGTGACC CCCGTCATCG AGCAGATCCT GCCTGACTCC
    CCTGGCTCCC ACCTGCCCCA CACCGTCACC CTGGTGTCCA TCCCAGCCTC ATCTCATGGC
    AAGCGTGGAC TTTCAGTCTC CATTGACCCC CCGCACGGCC CACCGGGCTG TGGCACCGAG
    CACTCACACA CCGTCCGCGT CCAGGGAGTG GATCCGGGCT GCATGAGCCC AGATGTGAAG
    AATTCCATCC ACGTCGGAGA CCGGATCTTG GAAATCAATG GCACGCCCAT CCGAAATGTG
    CCCCTGGACG AGATTGACCT GCTGATTCAG GAAACCAGCC GCCTGCTCCA GCTGACCCTC
    GAGCATGACC CTCACGATAC ACTGGGCCAC GGGCTGGGGC CTGAGACCAG CCCCCTGAGC
    TCTCCGGCTT ATACTCCCAG CGGGGAGGCG GGCAGCTCTG CCCGGCAGAA ACCTGTCTTG
    AGGAGCTGCA GCATCGACAG GTCTCCGGGC GCTGGCTCAC TGGGCTCCCC GGCCTCCCAG
    CGCAAGGACC TGGGTCGCTC TGAGTCCCTC CGCGTAGTCT GCCGGCCACA CCGCATCTTC
    CGGCCGTCGG ACCTCATCCA CGGGGAGGTG CTGGGCAAGG GCTGCTTCGG CCAGGCTATC
    AAGGTGACAC ACCGTGAGAC AGGTGAGGTG ATGGTGATGA AGGAGCTGAT CCGGTTCGAC
    GAGGAGACCC AGAGGACGTT CCTCAAGGAG GTGAAGGTCA TGCGATGCCT GGAACACCCC
    AACGTGCTCA AGTTCATCGG GGTGCTCTAC AAGGACAAGA GGCTCAACTT CATCACTGAG
    TACATCAAGG GCGGCACGCT CCGGGGCATC ATCAAGAGCA TGGACAGCCA GTACCCATGG
    AGCCAGAGAG TGAGCTTTGC CAAGGACATC GCATCAGGGA TGGCCTACCT CCACTCCATG
    AACATCATCC ACCGAGACCT CAACTCCCAC AACTGCCTGG TCCGCGAGAA CAAGAATGTG
    GTGGTGGCTG ACTTCGGGCT GGCGCGTCTC ATGGTGGACG AGAAGACTCA GCCTGAGGGC
    CTGCGGAGCC TCAAGAAGCC AGACCGCAAG AAGCGCTACA CCGTGGTGGG CAACCCCTAC
    TGGATGGCAC CTGAGATGAT CAACGGCCGC AGCTATGATG AGAAGGTGGA TGTGTTCTCC
    TTTGGGATCG TCCTGTGCGA GATCATCGGG CGGGTGAACG CAGACCCTGA CTACCTGCCC
    CGCACCATGG ACTTTGGCCT CAACGTGCGA GGATTCCTGG ACCGCTACTG CCCCCCAAAC
    TGCCCCCCGA GCTTCTTCCC CATCACCGTG CGCTGTTGCG ATCTGGACCC CGAGAAGAGG
    CCATCCTTTG TGAAGCTGGA ACACTGGCTG GAGACCCTCC GCATGCACCT GGCCGGCCAC
    CTGCCACTGG GCCCACAGCT GGAGCAGCTG GACAGAGGTT TCTGGGAGAC CTACCGGCGC
    GGCGAGAGCG GACTGCCTGC CCACCCTGAG GTCCCCGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001191355.1
    CDS34..1875
    Translation

    Target ORF information:

    RefSeq Version NM_001204426.2
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM domain kinase 1 (LIMK1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001204426.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCTGTTGG CTTCAGCCCC AAGAAGACGC CGCTTCCTCC AGAGGGCTAA GTGTTGTGAC 
    TGCAGTGCCT CCCTGTCGCA CCAGTACTAT GAGAAGGATG GGCAGCTCTT CTGCAAGAAG
    GACTACTGGG CCCGCTATGG CGAGTCCTGC CATGGGTGCT CTGAGCAAAT CACCAAGGGA
    CTGGTTATGG TGGCTGGGGA GCTGAAGTAC CACCCCGAGT GTTTCATCTG CCTCACGTGT
    GGGACCTTTA TCGGTGACGG GGACACCTAC ACGCTGGTGG AGCACTCCAA GCTGTACTGC
    GGGCACTGCT ACTACCAGAC TGTGGTGACC CCCGTCATCG AGCAGATCCT GCCTGACTCC
    CCTGGCTCCC ACCTGCCCCA CACCGTCACC CTGGTGTCCA TCCCAGCCTC ATCTCATGGC
    AAGCGTGGAC TTTCAGTCTC CATTGACCCC CCGCACGGCC CACCGGGCTG TGGCACCGAG
    CACTCACACA CCGTCCGCGT CCAGGGAGTG GATCCGGGCT GCATGAGCCC AGATGTGAAG
    AATTCCATCC ACGTCGGAGA CCGGATCTTG GAAATCAATG GCACGCCCAT CCGAAATGTG
    CCCCTGGACG AGATTGACCT GCTGATTCAG GAAACCAGCC GCCTGCTCCA GCTGACCCTC
    GAGCATGACC CTCACGATAC ACTGGGCCAC GGGCTGGGGC CTGAGACCAG CCCCCTGAGC
    TCTCCGGCTT ATACTCCCAG CGGGGAGGCG GGCAGCTCTG CCCGGCAGAA ACCTGTCTTG
    AGGAGCTGCA GCATCGACAG GTCTCCGGGC GCTGGCTCAC TGGGCTCCCC GGCCTCCCAG
    CGCAAGGACC TGGGTCGCTC TGAGTCCCTC CGCGTAGTCT GCCGGCCACA CCGCATCTTC
    CGGCCGTCGG ACCTCATCCA CGGGGAGGTG CTGGGCAAGG GCTGCTTCGG CCAGGCTATC
    AAGGTGACAC ACCGTGAGAC AGGTGAGGTG ATGGTGATGA AGGAGCTGAT CCGGTTCGAC
    GAGGAGACCC AGAGGACGTT CCTCAAGGAG GTGAAGGTCA TGCGATGCCT GGAACACCCC
    AACGTGCTCA AGTTCATCGG GGTGCTCTAC AAGGACAAGA GGCTCAACTT CATCACTGAG
    TACATCAAGG GCGGCACGCT CCGGGGCATC ATCAAGAGCA TGGACAGCCA GTACCCATGG
    AGCCAGAGAG TGAGCTTTGC CAAGGACATC GCATCAGGGA TGGCCTACCT CCACTCCATG
    AACATCATCC ACCGAGACCT CAACTCCCAC AACTGCCTGG TCCGCGAGAA CAAGAATGTG
    GTGGTGGCTG ACTTCGGGCT GGCGCGTCTC ATGGTGGACG AGAAGACTCA GCCTGAGGGC
    CTGCGGAGCC TCAAGAAGCC AGACCGCAAG AAGCGCTACA CCGTGGTGGG CAACCCCTAC
    TGGATGGCAC CTGAGATGAT CAACGGCCGC AGCTATGATG AGAAGGTGGA TGTGTTCTCC
    TTTGGGATCG TCCTGTGCGA GATCATCGGG CGGGTGAACG CAGACCCTGA CTACCTGCCC
    CGCACCATGG ACTTTGGCCT CAACGTGCGA GGATTCCTGG ACCGCTACTG CCCCCCAAAC
    TGCCCCCCGA GCTTCTTCCC CATCACCGTG CGCTGTTGCG ATCTGGACCC CGAGAAGAGG
    CCATCCTTTG TGAAGCTGGA ACACTGGCTG GAGACCCTCC GCATGCACCT GGCCGGCCAC
    CTGCCACTGG GCCCACAGCT GGAGCAGCTG GACAGAGGTT TCTGGGAGAC CTACCGGCGC
    GGCGAGAGCG GACTGCCTGC CCACCCTGAG GTCCCCGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22318
    Clone ID Related Accession (Same CDS sequence) NM_002314.4 , NM_002314.3
    Accession Version NM_002314.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1944bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-25
    Organism Homo sapiens(human)
    Product LIM domain kinase 1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005056.2, D26309.1 and AW016257.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_002314.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. The exon structure of the 5' UTR has been inferred from cross-species comparisons and lacks direct experimental support. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.8350.1, SRR1660805.38201.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000336180.7/ ENSP00000336740.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAGGTTGA CGCTACTTTG TTGCACCTGG AGGGAAGAAC GTATGGGAGA GGAAGGAAGC 
    GAGTTGCCCG TGTGTGCAAG CTGCGGCCAG AGGATCTATG ATGGCCAGTA CCTCCAGGCC
    CTGAACGCGG ACTGGCACGC AGACTGCTTC AGGTGTTGTG ACTGCAGTGC CTCCCTGTCG
    CACCAGTACT ATGAGAAGGA TGGGCAGCTC TTCTGCAAGA AGGACTACTG GGCCCGCTAT
    GGCGAGTCCT GCCATGGGTG CTCTGAGCAA ATCACCAAGG GACTGGTTAT GGTGGCTGGG
    GAGCTGAAGT ACCACCCCGA GTGTTTCATC TGCCTCACGT GTGGGACCTT TATCGGTGAC
    GGGGACACCT ACACGCTGGT GGAGCACTCC AAGCTGTACT GCGGGCACTG CTACTACCAG
    ACTGTGGTGA CCCCCGTCAT CGAGCAGATC CTGCCTGACT CCCCTGGCTC CCACCTGCCC
    CACACCGTCA CCCTGGTGTC CATCCCAGCC TCATCTCATG GCAAGCGTGG ACTTTCAGTC
    TCCATTGACC CCCCGCACGG CCCACCGGGC TGTGGCACCG AGCACTCACA CACCGTCCGC
    GTCCAGGGAG TGGATCCGGG CTGCATGAGC CCAGATGTGA AGAATTCCAT CCACGTCGGA
    GACCGGATCT TGGAAATCAA TGGCACGCCC ATCCGAAATG TGCCCCTGGA CGAGATTGAC
    CTGCTGATTC AGGAAACCAG CCGCCTGCTC CAGCTGACCC TCGAGCATGA CCCTCACGAT
    ACACTGGGCC ACGGGCTGGG GCCTGAGACC AGCCCCCTGA GCTCTCCGGC TTATACTCCC
    AGCGGGGAGG CGGGCAGCTC TGCCCGGCAG AAACCTGTCT TGAGGAGCTG CAGCATCGAC
    AGGTCTCCGG GCGCTGGCTC ACTGGGCTCC CCGGCCTCCC AGCGCAAGGA CCTGGGTCGC
    TCTGAGTCCC TCCGCGTAGT CTGCCGGCCA CACCGCATCT TCCGGCCGTC GGACCTCATC
    CACGGGGAGG TGCTGGGCAA GGGCTGCTTC GGCCAGGCTA TCAAGGTGAC ACACCGTGAG
    ACAGGTGAGG TGATGGTGAT GAAGGAGCTG ATCCGGTTCG ACGAGGAGAC CCAGAGGACG
    TTCCTCAAGG AGGTGAAGGT CATGCGATGC CTGGAACACC CCAACGTGCT CAAGTTCATC
    GGGGTGCTCT ACAAGGACAA GAGGCTCAAC TTCATCACTG AGTACATCAA GGGCGGCACG
    CTCCGGGGCA TCATCAAGAG CATGGACAGC CAGTACCCAT GGAGCCAGAG AGTGAGCTTT
    GCCAAGGACA TCGCATCAGG GATGGCCTAC CTCCACTCCA TGAACATCAT CCACCGAGAC
    CTCAACTCCC ACAACTGCCT GGTCCGCGAG AACAAGAATG TGGTGGTGGC TGACTTCGGG
    CTGGCGCGTC TCATGGTGGA CGAGAAGACT CAGCCTGAGG GCCTGCGGAG CCTCAAGAAG
    CCAGACCGCA AGAAGCGCTA CACCGTGGTG GGCAACCCCT ACTGGATGGC ACCTGAGATG
    ATCAACGGCC GCAGCTATGA TGAGAAGGTG GATGTGTTCT CCTTTGGGAT CGTCCTGTGC
    GAGATCATCG GGCGGGTGAA CGCAGACCCT GACTACCTGC CCCGCACCAT GGACTTTGGC
    CTCAACGTGC GAGGATTCCT GGACCGCTAC TGCCCCCCAA ACTGCCCCCC GAGCTTCTTC
    CCCATCACCG TGCGCTGTTG CGATCTGGAC CCCGAGAAGA GGCCATCCTT TGTGAAGCTG
    GAACACTGGC TGGAGACCCT CCGCATGCAC CTGGCCGGCC ACCTGCCACT GGGCCCACAG
    CTGGAGCAGC TGGACAGAGG TTTCTGGGAG ACCTACCGGC GCGGCGAGAG CGGACTGCCT
    GCCCACCCTG AGGTCCCCGA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_002305.1
    CDS188..2131
    Translation

    Target ORF information:

    RefSeq Version NM_002314.4
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM domain kinase 1 (LIMK1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_002314.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAGGTTGA CGCTACTTTG TTGCACCTGG AGGGAAGAAC GTATGGGAGA GGAAGGAAGC 
    GAGTTGCCCG TGTGTGCAAG CTGCGGCCAG AGGATCTATG ATGGCCAGTA CCTCCAGGCC
    CTGAACGCGG ACTGGCACGC AGACTGCTTC AGGTGTTGTG ACTGCAGTGC CTCCCTGTCG
    CACCAGTACT ATGAGAAGGA TGGGCAGCTC TTCTGCAAGA AGGACTACTG GGCCCGCTAT
    GGCGAGTCCT GCCATGGGTG CTCTGAGCAA ATCACCAAGG GACTGGTTAT GGTGGCTGGG
    GAGCTGAAGT ACCACCCCGA GTGTTTCATC TGCCTCACGT GTGGGACCTT TATCGGTGAC
    GGGGACACCT ACACGCTGGT GGAGCACTCC AAGCTGTACT GCGGGCACTG CTACTACCAG
    ACTGTGGTGA CCCCCGTCAT CGAGCAGATC CTGCCTGACT CCCCTGGCTC CCACCTGCCC
    CACACCGTCA CCCTGGTGTC CATCCCAGCC TCATCTCATG GCAAGCGTGG ACTTTCAGTC
    TCCATTGACC CCCCGCACGG CCCACCGGGC TGTGGCACCG AGCACTCACA CACCGTCCGC
    GTCCAGGGAG TGGATCCGGG CTGCATGAGC CCAGATGTGA AGAATTCCAT CCACGTCGGA
    GACCGGATCT TGGAAATCAA TGGCACGCCC ATCCGAAATG TGCCCCTGGA CGAGATTGAC
    CTGCTGATTC AGGAAACCAG CCGCCTGCTC CAGCTGACCC TCGAGCATGA CCCTCACGAT
    ACACTGGGCC ACGGGCTGGG GCCTGAGACC AGCCCCCTGA GCTCTCCGGC TTATACTCCC
    AGCGGGGAGG CGGGCAGCTC TGCCCGGCAG AAACCTGTCT TGAGGAGCTG CAGCATCGAC
    AGGTCTCCGG GCGCTGGCTC ACTGGGCTCC CCGGCCTCCC AGCGCAAGGA CCTGGGTCGC
    TCTGAGTCCC TCCGCGTAGT CTGCCGGCCA CACCGCATCT TCCGGCCGTC GGACCTCATC
    CACGGGGAGG TGCTGGGCAA GGGCTGCTTC GGCCAGGCTA TCAAGGTGAC ACACCGTGAG
    ACAGGTGAGG TGATGGTGAT GAAGGAGCTG ATCCGGTTCG ACGAGGAGAC CCAGAGGACG
    TTCCTCAAGG AGGTGAAGGT CATGCGATGC CTGGAACACC CCAACGTGCT CAAGTTCATC
    GGGGTGCTCT ACAAGGACAA GAGGCTCAAC TTCATCACTG AGTACATCAA GGGCGGCACG
    CTCCGGGGCA TCATCAAGAG CATGGACAGC CAGTACCCAT GGAGCCAGAG AGTGAGCTTT
    GCCAAGGACA TCGCATCAGG GATGGCCTAC CTCCACTCCA TGAACATCAT CCACCGAGAC
    CTCAACTCCC ACAACTGCCT GGTCCGCGAG AACAAGAATG TGGTGGTGGC TGACTTCGGG
    CTGGCGCGTC TCATGGTGGA CGAGAAGACT CAGCCTGAGG GCCTGCGGAG CCTCAAGAAG
    CCAGACCGCA AGAAGCGCTA CACCGTGGTG GGCAACCCCT ACTGGATGGC ACCTGAGATG
    ATCAACGGCC GCAGCTATGA TGAGAAGGTG GATGTGTTCT CCTTTGGGAT CGTCCTGTGC
    GAGATCATCG GGCGGGTGAA CGCAGACCCT GACTACCTGC CCCGCACCAT GGACTTTGGC
    CTCAACGTGC GAGGATTCCT GGACCGCTAC TGCCCCCCAA ACTGCCCCCC GAGCTTCTTC
    CCCATCACCG TGCGCTGTTG CGATCTGGAC CCCGAGAAGA GGCCATCCTT TGTGAAGCTG
    GAACACTGGC TGGAGACCCT CCGCATGCAC CTGGCCGGCC ACCTGCCACT GGGCCCACAG
    CTGGAGCAGC TGGACAGAGG TTTCTGGGAG ACCTACCGGC GCGGCGAGAG CGGACTGCCT
    GCCCACCCTG AGGTCCCCGA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22318
    Clone ID Related Accession (Same CDS sequence) NM_002314.4 , NM_002314.3
    Accession Version NM_002314.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1944bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-05
    Organism Homo sapiens(human)
    Product LIM domain kinase 1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005056.2, D26309.1 and AW016257.1. This sequence is a reference standard in the RefSeqGene project. On Mar 1, 2011 this sequence version replaced NM_002314.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. The exon structure of the 5' UTR has been inferred from cross-species comparisons and lacks direct experimental support. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.8350.1, SRR1660805.38201.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAGGTTGA CGCTACTTTG TTGCACCTGG AGGGAAGAAC GTATGGGAGA GGAAGGAAGC 
    GAGTTGCCCG TGTGTGCAAG CTGCGGCCAG AGGATCTATG ATGGCCAGTA CCTCCAGGCC
    CTGAACGCGG ACTGGCACGC AGACTGCTTC AGGTGTTGTG ACTGCAGTGC CTCCCTGTCG
    CACCAGTACT ATGAGAAGGA TGGGCAGCTC TTCTGCAAGA AGGACTACTG GGCCCGCTAT
    GGCGAGTCCT GCCATGGGTG CTCTGAGCAA ATCACCAAGG GACTGGTTAT GGTGGCTGGG
    GAGCTGAAGT ACCACCCCGA GTGTTTCATC TGCCTCACGT GTGGGACCTT TATCGGTGAC
    GGGGACACCT ACACGCTGGT GGAGCACTCC AAGCTGTACT GCGGGCACTG CTACTACCAG
    ACTGTGGTGA CCCCCGTCAT CGAGCAGATC CTGCCTGACT CCCCTGGCTC CCACCTGCCC
    CACACCGTCA CCCTGGTGTC CATCCCAGCC TCATCTCATG GCAAGCGTGG ACTTTCAGTC
    TCCATTGACC CCCCGCACGG CCCACCGGGC TGTGGCACCG AGCACTCACA CACCGTCCGC
    GTCCAGGGAG TGGATCCGGG CTGCATGAGC CCAGATGTGA AGAATTCCAT CCACGTCGGA
    GACCGGATCT TGGAAATCAA TGGCACGCCC ATCCGAAATG TGCCCCTGGA CGAGATTGAC
    CTGCTGATTC AGGAAACCAG CCGCCTGCTC CAGCTGACCC TCGAGCATGA CCCTCACGAT
    ACACTGGGCC ACGGGCTGGG GCCTGAGACC AGCCCCCTGA GCTCTCCGGC TTATACTCCC
    AGCGGGGAGG CGGGCAGCTC TGCCCGGCAG AAACCTGTCT TGAGGAGCTG CAGCATCGAC
    AGGTCTCCGG GCGCTGGCTC ACTGGGCTCC CCGGCCTCCC AGCGCAAGGA CCTGGGTCGC
    TCTGAGTCCC TCCGCGTAGT CTGCCGGCCA CACCGCATCT TCCGGCCGTC GGACCTCATC
    CACGGGGAGG TGCTGGGCAA GGGCTGCTTC GGCCAGGCTA TCAAGGTGAC ACACCGTGAG
    ACAGGTGAGG TGATGGTGAT GAAGGAGCTG ATCCGGTTCG ACGAGGAGAC CCAGAGGACG
    TTCCTCAAGG AGGTGAAGGT CATGCGATGC CTGGAACACC CCAACGTGCT CAAGTTCATC
    GGGGTGCTCT ACAAGGACAA GAGGCTCAAC TTCATCACTG AGTACATCAA GGGCGGCACG
    CTCCGGGGCA TCATCAAGAG CATGGACAGC CAGTACCCAT GGAGCCAGAG AGTGAGCTTT
    GCCAAGGACA TCGCATCAGG GATGGCCTAC CTCCACTCCA TGAACATCAT CCACCGAGAC
    CTCAACTCCC ACAACTGCCT GGTCCGCGAG AACAAGAATG TGGTGGTGGC TGACTTCGGG
    CTGGCGCGTC TCATGGTGGA CGAGAAGACT CAGCCTGAGG GCCTGCGGAG CCTCAAGAAG
    CCAGACCGCA AGAAGCGCTA CACCGTGGTG GGCAACCCCT ACTGGATGGC ACCTGAGATG
    ATCAACGGCC GCAGCTATGA TGAGAAGGTG GATGTGTTCT CCTTTGGGAT CGTCCTGTGC
    GAGATCATCG GGCGGGTGAA CGCAGACCCT GACTACCTGC CCCGCACCAT GGACTTTGGC
    CTCAACGTGC GAGGATTCCT GGACCGCTAC TGCCCCCCAA ACTGCCCCCC GAGCTTCTTC
    CCCATCACCG TGCGCTGTTG CGATCTGGAC CCCGAGAAGA GGCCATCCTT TGTGAAGCTG
    GAACACTGGC TGGAGACCCT CCGCATGCAC CTGGCCGGCC ACCTGCCACT GGGCCCACAG
    CTGGAGCAGC TGGACAGAGG TTTCTGGGAG ACCTACCGGC GCGGCGAGAG CGGACTGCCT
    GCCCACCCTG AGGTCCCCGA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_002305.1
    CDS215..2158
    Translation

    Target ORF information:

    RefSeq Version NM_002314.3
    Organism Homo sapiens(human)
    Definition Homo sapiens LIM domain kinase 1 (LIMK1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_002314.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAGGTTGA CGCTACTTTG TTGCACCTGG AGGGAAGAAC GTATGGGAGA GGAAGGAAGC 
    GAGTTGCCCG TGTGTGCAAG CTGCGGCCAG AGGATCTATG ATGGCCAGTA CCTCCAGGCC
    CTGAACGCGG ACTGGCACGC AGACTGCTTC AGGTGTTGTG ACTGCAGTGC CTCCCTGTCG
    CACCAGTACT ATGAGAAGGA TGGGCAGCTC TTCTGCAAGA AGGACTACTG GGCCCGCTAT
    GGCGAGTCCT GCCATGGGTG CTCTGAGCAA ATCACCAAGG GACTGGTTAT GGTGGCTGGG
    GAGCTGAAGT ACCACCCCGA GTGTTTCATC TGCCTCACGT GTGGGACCTT TATCGGTGAC
    GGGGACACCT ACACGCTGGT GGAGCACTCC AAGCTGTACT GCGGGCACTG CTACTACCAG
    ACTGTGGTGA CCCCCGTCAT CGAGCAGATC CTGCCTGACT CCCCTGGCTC CCACCTGCCC
    CACACCGTCA CCCTGGTGTC CATCCCAGCC TCATCTCATG GCAAGCGTGG ACTTTCAGTC
    TCCATTGACC CCCCGCACGG CCCACCGGGC TGTGGCACCG AGCACTCACA CACCGTCCGC
    GTCCAGGGAG TGGATCCGGG CTGCATGAGC CCAGATGTGA AGAATTCCAT CCACGTCGGA
    GACCGGATCT TGGAAATCAA TGGCACGCCC ATCCGAAATG TGCCCCTGGA CGAGATTGAC
    CTGCTGATTC AGGAAACCAG CCGCCTGCTC CAGCTGACCC TCGAGCATGA CCCTCACGAT
    ACACTGGGCC ACGGGCTGGG GCCTGAGACC AGCCCCCTGA GCTCTCCGGC TTATACTCCC
    AGCGGGGAGG CGGGCAGCTC TGCCCGGCAG AAACCTGTCT TGAGGAGCTG CAGCATCGAC
    AGGTCTCCGG GCGCTGGCTC ACTGGGCTCC CCGGCCTCCC AGCGCAAGGA CCTGGGTCGC
    TCTGAGTCCC TCCGCGTAGT CTGCCGGCCA CACCGCATCT TCCGGCCGTC GGACCTCATC
    CACGGGGAGG TGCTGGGCAA GGGCTGCTTC GGCCAGGCTA TCAAGGTGAC ACACCGTGAG
    ACAGGTGAGG TGATGGTGAT GAAGGAGCTG ATCCGGTTCG ACGAGGAGAC CCAGAGGACG
    TTCCTCAAGG AGGTGAAGGT CATGCGATGC CTGGAACACC CCAACGTGCT CAAGTTCATC
    GGGGTGCTCT ACAAGGACAA GAGGCTCAAC TTCATCACTG AGTACATCAA GGGCGGCACG
    CTCCGGGGCA TCATCAAGAG CATGGACAGC CAGTACCCAT GGAGCCAGAG AGTGAGCTTT
    GCCAAGGACA TCGCATCAGG GATGGCCTAC CTCCACTCCA TGAACATCAT CCACCGAGAC
    CTCAACTCCC ACAACTGCCT GGTCCGCGAG AACAAGAATG TGGTGGTGGC TGACTTCGGG
    CTGGCGCGTC TCATGGTGGA CGAGAAGACT CAGCCTGAGG GCCTGCGGAG CCTCAAGAAG
    CCAGACCGCA AGAAGCGCTA CACCGTGGTG GGCAACCCCT ACTGGATGGC ACCTGAGATG
    ATCAACGGCC GCAGCTATGA TGAGAAGGTG GATGTGTTCT CCTTTGGGAT CGTCCTGTGC
    GAGATCATCG GGCGGGTGAA CGCAGACCCT GACTACCTGC CCCGCACCAT GGACTTTGGC
    CTCAACGTGC GAGGATTCCT GGACCGCTAC TGCCCCCCAA ACTGCCCCCC GAGCTTCTTC
    CCCATCACCG TGCGCTGTTG CGATCTGGAC CCCGAGAAGA GGCCATCCTT TGTGAAGCTG
    GAACACTGGC TGGAGACCCT CCGCATGCAC CTGGCCGGCC ACCTGCCACT GGGCCCACAG
    CTGGAGCAGC TGGACAGAGG TTTCTGGGAG ACCTACCGGC GCGGCGAGAG CGGACTGCCT
    GCCCACCCTG AGGTCCCCGA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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