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MMP13 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol MMP13
Entrez Gene ID 4322
Full Name matrix metallopeptidase 13
Synonyms CLG3,MANDP1,MDST,MMP-13
General protein information
Preferred Names

matrix metallopeptidase 13

Names

collagenase 3
matrix metalloproteinase 13 (collagenase 3)

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

11

11q22.2

Summary This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. Mutations in this gene are associated with metaphyseal anadysplasia. This gene is part of a cluster of MMP genes on chromosome 11. [provided by RefSeq, Jan 2016].
Disorder MIM:

600108

Disorder Html: Spondyloepimetaphyseal dysplasia, Missouri type, 602111 (3);

mRNA and Protein(s)

mRNA Protein Name
NM_002427.3 NP_002418.1 collagenase 3 preproprotein



Pan troglodytes (chimpanzee) MMP13 XP_001154361.1
Danio rerio (zebrafish) mmp13b XP_001345507.1
Gallus gallus (chicken) MMP13 XP_003640635.1
Xenopus tropicalis (tropical clawed frog) LOC100490019 XP_002934971.1
Canis lupus familiaris (dog) MMP13 XP_536598.3
Mus musculus (house mouse) Mmp13 NP_032633.1
Homo sapiens (human) MMP13 NP_002418.1
Rattus norvegicus (Norway rat) Mmp13 NP_598214.1
Caenorhabditis elegans (roundworm) CELE_C31B8.8 NP_503790.1
Bos taurus (cattle) MMP13 NP_776814.1
Macaca mulatta (rhesus monkey) MMP13 XP_001098996.1


Related articles in PubMed

Hydrostatic Pressure Regulates MicroRNA Expression Levels in Osteoarthritic Chondrocyte Cultures via the Wnt/β-Catenin Pathway.
Cheleschi S, De Palma A, Pecorelli A, Pascarelli NA, Valacchi G, Belmonte G, Carta S, Galeazzi M, Fioravanti A
International journal of molecular sciences18(1)(2017 Jan)

Transforming growth factor-β1 regulation of ATF-3, c-Jun and JunB proteins for activation of matrix metalloproteinase-13 gene in human breast cancer cells.
Gokulnath M, Swetha R, Thejaswini G, Shilpa P, Selvamurugan N
International journal of biological macromolecules94(Pt A)370-377(2017 Jan)

Serum and knee synovial fluid matrixmetalloproteinase-13 and tumor necrosis factor-alpha levels in patients with late stage osteoarthritis.
Özler K, Aktaş E, Atay Ç, Yılmaz B, Arıkan M, Güngör Ş
Acta orthopaedica et traumatologica turcica50(6)670-673(2016 Dec)

The NAD-Dependent Deacetylase Sirtuin-1 Regulates the Expression of Osteogenic Transcriptional Activator Runt-Related Transcription Factor 2 (Runx2) and Production of Matrix Metalloproteinase (MMP)-13 in Chondrocytes in Osteoarthritis.
Terauchi K, Kobayashi H, Yatabe K, Yui N, Fujiya H, Niki H, Musha H, Yudoh K
International journal of molecular sciences17(7)(2016 Jun)

Matrix Metalloproteinase-13 - A Potential Biomarker for Detection and Prognostic Assessment of Patients with Esophageal Squamous Cell Carcinoma.
Sedighi M, Aledavood SA, Abbaszadegan M, Memar B, Montazer M, Rajabian M, Gholamin M
Asian Pacific journal of cancer prevention : APJCP17(6)2781-5(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Endoplasmic reticulum stress participates in the progress of senescence and apoptosis of osteoarthritic chondrocytes, which manifested in increased expression of ADAMTS5, MMP13, and decreased COL2A1 expression.
Title: Endoplasmic reticulum stress participates in the progress of senescence and apoptosis of osteoarthritis chondrocytes.

Oxytocin prevents cartilage matrix destruction via regulating MMP1 and MMP13.
Title: Oxytocin prevents cartilage matrix destruction via regulating matrix metalloproteinases.

these findings support roles for both cFOS (indirect) and ATF3 (direct) in effecting MMP13 transcription in human chondrocytes.
Title: Cytokine-induced MMP13 Expression in Human Chondrocytes Is Dependent on Activating Transcription Factor 3 (ATF3) Regulation.

In osteoarthritis (OA) chondrocytes, hydrostatic pressure (HP) restores the expression levels of some miRNAs, downregulates MMP-13, ADAMTS-5, and HDAC-4, and modulates the Wnt/beta-catenin pathway activation.
Title: Hydrostatic Pressure Regulates MicroRNA Expression Levels in Osteoarthritic Chondrocyte Cultures via the Wnt/β-Catenin Pathway.

As the grade of late stage osteoarthritis advances, increase in synovial MMP-13 and serum TNF-a levels become pronounced.
Title: Serum and knee synovial fluid matrixmetalloproteinase-13 and tumor necrosis factor-alpha levels in patients with late stage osteoarthritis.

The following MMP13 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MMP13 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu19625 NM_002427.3
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Homo sapiens matrix metallopeptidase 13 (MMP13), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu19625
Clone ID Related Accession (Same CDS sequence) NM_002427.3
Accession Version NM_002427.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1416bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-06
Organism Homo sapiens(Human)
Product collagenase 3 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK292211.1, AK222748.1, X75308.1 and CA413743.1. This sequence is a reference standard in the RefSeqGene project. On May 13, 2010 this sequence version replaced NM_002427.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK222748.1, X75308.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02400289 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCATCCAG GGGTCCTGGC TGCCTTCCTC TTCTTGAGCT GGACTCATTG TCGGGCCCTG 
CCCCTTCCCA GTGGTGGTGA TGAAGATGAT TTGTCTGAGG AAGACCTCCA GTTTGCAGAG
CGCTACCTGA GATCATACTA CCATCCTACA AATCTCGCGG GAATCCTGAA GGAGAATGCA
GCAAGCTCCA TGACTGAGAG GCTCCGAGAA ATGCAGTCTT TCTTCGGCTT AGAGGTGACT
GGCAAACTTG ACGATAACAC CTTAGATGTC ATGAAAAAGC CAAGATGCGG GGTTCCTGAT
GTGGGTGAAT ACAATGTTTT CCCTCGAACT CTTAAATGGT CCAAAATGAA TTTAACCTAC
AGAATTGTGA ATTACACCCC TGATATGACT CATTCTGAAG TCGAAAAGGC ATTCAAAAAA
GCCTTCAAAG TTTGGTCCGA TGTAACTCCT CTGAATTTTA CCAGACTTCA CGATGGCATT
GCTGACATCA TGATCTCTTT TGGAATTAAG GAGCATGGCG ACTTCTACCC ATTTGATGGG
CCCTCTGGCC TGCTGGCTCA TGCTTTTCCT CCTGGGCCAA ATTATGGAGG AGATGCCCAT
TTTGATGATG ATGAAACCTG GACAAGTAGT TCCAAAGGCT ACAACTTGTT TCTTGTTGCT
GCGCATGAGT TCGGCCACTC CTTAGGTCTT GACCACTCCA AGGACCCTGG AGCACTCATG
TTTCCTATCT ACACCTACAC CGGCAAAAGC CACTTTATGC TTCCTGATGA CGATGTACAA
GGGATCCAGT CTCTCTATGG TCCAGGAGAT GAAGACCCCA ACCCTAAACA TCCAAAAACG
CCAGACAAAT GTGACCCTTC CTTATCCCTT GATGCCATTA CCAGTCTCCG AGGAGAAACA
ATGATCTTTA AAGACAGATT CTTCTGGCGC CTGCATCCTC AGCAGGTTGA TGCGGAGCTG
TTTTTAACGA AATCATTTTG GCCAGAACTT CCCAACCGTA TTGATGCTGC ATATGAGCAC
CCTTCTCATG ACCTCATCTT CATCTTCAGA GGTAGAAAAT TTTGGGCTCT TAATGGTTAT
GACATTCTGG AAGGTTATCC CAAAAAAATA TCTGAACTGG GTCTTCCAAA AGAAGTTAAG
AAGATAAGTG CAGCTGTTCA CTTTGAGGAT ACAGGCAAGA CTCTCCTGTT CTCAGGAAAC
CAGGTCTGGA GATATGATGA TACTAACCAT ATTATGGATA AAGACTATCC GAGACTAATA
GAAGAAGACT TCCCAGGAAT TGGTGATAAA GTAGATGCTG TCTATGAGAA AAATGGTTAT
ATCTATTTTT TCAACGGACC CATACAGTTT GAATACAGCA TCTGGAGTAA CCGTATTGTT
CGCGTCATGC CAGCAAATTC CATTTTGTGG TGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002418.1
CDS30..1445
Misc Feature(1)123..302
Misc Feature(2)189..191
Misc Feature(3)309..332
Misc Feature(4)336..338
Misc Feature(5)363..830
Misc Feature(6)363..830
Misc Feature(7)order(519..521,555..557,576..596,621..623,693..698,723..725,753..761)
Misc Feature(8)555..767
Misc Feature(9)order(693..698,705..707,723..725)
Misc Feature(10)831..1442
Misc Feature(11)870..1442
Misc Feature(12)order(900..902,906..908,1032..1034,1038..1040,1176..1178,1182..1184,1323..1325,1329..1331)
Misc Feature(13)1125..1127
Exon (1)1..149
Exon (2)150..391
Exon (3)392..540
Exon (4)541..666
Exon (5)667..828
Exon (6)829..946
Exon (7)947..1080
Exon (8)1081..1240
Exon (9)1241..1344
Exon (10)1345..2719
Translation

Target ORF information:

RefSeq Version NM_002427.3
Organism Homo sapiens(Human)
Definition Homo sapiens matrix metallopeptidase 13 (MMP13), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002427.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCATCCAG GGGTCCTGGC TGCCTTCCTC TTCTTGAGCT GGACTCATTG TCGGGCCCTG 
CCCCTTCCCA GTGGTGGTGA TGAAGATGAT TTGTCTGAGG AAGACCTCCA GTTTGCAGAG
CGCTACCTGA GATCATACTA CCATCCTACA AATCTCGCGG GAATCCTGAA GGAGAATGCA
GCAAGCTCCA TGACTGAGAG GCTCCGAGAA ATGCAGTCTT TCTTCGGCTT AGAGGTGACT
GGCAAACTTG ACGATAACAC CTTAGATGTC ATGAAAAAGC CAAGATGCGG GGTTCCTGAT
GTGGGTGAAT ACAATGTTTT CCCTCGAACT CTTAAATGGT CCAAAATGAA TTTAACCTAC
AGAATTGTGA ATTACACCCC TGATATGACT CATTCTGAAG TCGAAAAGGC ATTCAAAAAA
GCCTTCAAAG TTTGGTCCGA TGTAACTCCT CTGAATTTTA CCAGACTTCA CGATGGCATT
GCTGACATCA TGATCTCTTT TGGAATTAAG GAGCATGGCG ACTTCTACCC ATTTGATGGG
CCCTCTGGCC TGCTGGCTCA TGCTTTTCCT CCTGGGCCAA ATTATGGAGG AGATGCCCAT
TTTGATGATG ATGAAACCTG GACAAGTAGT TCCAAAGGCT ACAACTTGTT TCTTGTTGCT
GCGCATGAGT TCGGCCACTC CTTAGGTCTT GACCACTCCA AGGACCCTGG AGCACTCATG
TTTCCTATCT ACACCTACAC CGGCAAAAGC CACTTTATGC TTCCTGATGA CGATGTACAA
GGGATCCAGT CTCTCTATGG TCCAGGAGAT GAAGACCCCA ACCCTAAACA TCCAAAAACG
CCAGACAAAT GTGACCCTTC CTTATCCCTT GATGCCATTA CCAGTCTCCG AGGAGAAACA
ATGATCTTTA AAGACAGATT CTTCTGGCGC CTGCATCCTC AGCAGGTTGA TGCGGAGCTG
TTTTTAACGA AATCATTTTG GCCAGAACTT CCCAACCGTA TTGATGCTGC ATATGAGCAC
CCTTCTCATG ACCTCATCTT CATCTTCAGA GGTAGAAAAT TTTGGGCTCT TAATGGTTAT
GACATTCTGG AAGGTTATCC CAAAAAAATA TCTGAACTGG GTCTTCCAAA AGAAGTTAAG
AAGATAAGTG CAGCTGTTCA CTTTGAGGAT ACAGGCAAGA CTCTCCTGTT CTCAGGAAAC
CAGGTCTGGA GATATGATGA TACTAACCAT ATTATGGATA AAGACTATCC GAGACTAATA
GAAGAAGACT TCCCAGGAAT TGGTGATAAA GTAGATGCTG TCTATGAGAA AAATGGTTAT
ATCTATTTTT TCAACGGACC CATACAGTTT GAATACAGCA TCTGGAGTAA CCGTATTGTT
CGCGTCATGC CAGCAAATTC CATTTTGTGG TGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Hydrostatic Pressure Regulates MicroRNA Expression Levels in Osteoarthritic Chondrocyte Cultures via the Wnt/β-Catenin Pathway.
International journal of molecular sciences18(1)(2017 Jan)
Cheleschi S,De Palma A,Pecorelli A,Pascarelli NA,Valacchi G,Belmonte G,Carta S,Galeazzi M,Fioravanti A


book

Transforming growth factor-β1 regulation of ATF-3, c-Jun and JunB proteins for activation of matrix metalloproteinase-13 gene in human breast cancer cells.
International journal of biological macromolecules94(Pt A)370-377(2017 Jan)
Gokulnath M,Swetha R,Thejaswini G,Shilpa P,Selvamurugan N


book

Serum and knee synovial fluid matrixmetalloproteinase-13 and tumor necrosis factor-alpha levels in patients with late stage osteoarthritis.
Acta orthopaedica et traumatologica turcica50(6)670-673(2016 Dec)
Özler K,Aktaş E,Atay Ç,Yılmaz B,Arıkan M,Güngör Ş


book

The NAD-Dependent Deacetylase Sirtuin-1 Regulates the Expression of Osteogenic Transcriptional Activator Runt-Related Transcription Factor 2 (Runx2) and Production of Matrix Metalloproteinase (MMP)-13 in Chondrocytes in Osteoarthritis.
International journal of molecular sciences17(7)(2016 Jun)
Terauchi K,Kobayashi H,Yatabe K,Yui N,Fujiya H,Niki H,Musha H,Yudoh K


book

Matrix Metalloproteinase-13 - A Potential Biomarker for Detection and Prognostic Assessment of Patients with Esophageal Squamous Cell Carcinoma.
Asian Pacific journal of cancer prevention : APJCP17(6)2781-5(2016)
Sedighi M,Aledavood SA,Abbaszadegan M,Memar B,Montazer M,Rajabian M,Gholamin M


 
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