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MMP13 cDNA ORF clone, Homo sapiens (human)

Gene Symbol MMP13
Entrez Gene ID 4322
Full Name matrix metallopeptidase 13
Synonyms CLG3, MANDP1, MMP-13
General protein information
Preferred Names
collagenase 3
Names
collagenase 3
MMP-13
matrix metalloproteinase 13 (collagenase 3)
NP_002418.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

11

11q22.3

Summary Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

600108

Disorder Html: Spondyloepimetaphyseal dysplasia, Missouri type, 602111 (3);

mRNA and Protein(s)

mRNA Protein Name
NM_002427 NP_002418 collagenase 3 preproprotein



Homo sapiens (human) MMP13 NP_002418.1
Pan troglodytes (chimpanzee) MMP13 XP_001154361.1
Macaca mulatta (Rhesus monkey) MMP13 XP_001098996.1
Canis lupus familiaris (dog) MMP13 XP_536598.3
Bos taurus (cattle) MMP13 NP_776814.1
Mus musculus (house mouse) Mmp13 NP_032633.1
Rattus norvegicus (Norway rat) Mmp13 NP_598214.1
Gallus gallus (chicken) MMP13 XP_003640635.1
Danio rerio (zebrafish) mmp13b XP_001345507.1
Caenorhabditis elegans CELE_C31B8.8 NP_503790.1
Xenopus (Silurana) tropicalis (western clawed frog) LOC100490019 XP_002934971.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MMP13 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MMP13 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu19625
NM_002427 Homo sapiens matrix metallopeptidase 13 (MMP13), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu19625
Clone ID Related Accession (Same CDS sequence) NM_002427
Accession Version NM_002427.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1416bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product collagenase 3 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK292211.1, AK222748.1, X75308.1 and CA413743.1. This sequence is a reference standard in the RefSeqGene project. On May 13, 2010 this sequence version replaced gi:13027796. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK222748.1, X75308.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA962338 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCATCCAG GGGTCCTGGC TGCCTTCCTC TTCTTGAGCT GGACTCATTG TCGGGCCCTG 
CCCCTTCCCA GTGGTGGTGA TGAAGATGAT TTGTCTGAGG AAGACCTCCA GTTTGCAGAG
CGCTACCTGA GATCATACTA CCATCCTACA AATCTCGCGG GAATCCTGAA GGAGAATGCA
GCAAGCTCCA TGACTGAGAG GCTCCGAGAA ATGCAGTCTT TCTTCGGCTT AGAGGTGACT
GGCAAACTTG ACGATAACAC CTTAGATGTC ATGAAAAAGC CAAGATGCGG GGTTCCTGAT
GTGGGTGAAT ACAATGTTTT CCCTCGAACT CTTAAATGGT CCAAAATGAA TTTAACCTAC
AGAATTGTGA ATTACACCCC TGATATGACT CATTCTGAAG TCGAAAAGGC ATTCAAAAAA
GCCTTCAAAG TTTGGTCCGA TGTAACTCCT CTGAATTTTA CCAGACTTCA CGATGGCATT
GCTGACATCA TGATCTCTTT TGGAATTAAG GAGCATGGCG ACTTCTACCC ATTTGATGGG
CCCTCTGGCC TGCTGGCTCA TGCTTTTCCT CCTGGGCCAA ATTATGGAGG AGATGCCCAT
TTTGATGATG ATGAAACCTG GACAAGTAGT TCCAAAGGCT ACAACTTGTT TCTTGTTGCT
GCGCATGAGT TCGGCCACTC CTTAGGTCTT GACCACTCCA AGGACCCTGG AGCACTCATG
TTTCCTATCT ACACCTACAC CGGCAAAAGC CACTTTATGC TTCCTGATGA CGATGTACAA
GGGATCCAGT CTCTCTATGG TCCAGGAGAT GAAGACCCCA ACCCTAAACA TCCAAAAACG
CCAGACAAAT GTGACCCTTC CTTATCCCTT GATGCCATTA CCAGTCTCCG AGGAGAAACA
ATGATCTTTA AAGACAGATT CTTCTGGCGC CTGCATCCTC AGCAGGTTGA TGCGGAGCTG
TTTTTAACGA AATCATTTTG GCCAGAACTT CCCAACCGTA TTGATGCTGC ATATGAGCAC
CCTTCTCATG ACCTCATCTT CATCTTCAGA GGTAGAAAAT TTTGGGCTCT TAATGGTTAT
GACATTCTGG AAGGTTATCC CAAAAAAATA TCTGAACTGG GTCTTCCAAA AGAAGTTAAG
AAGATAAGTG CAGCTGTTCA CTTTGAGGAT ACAGGCAAGA CTCTCCTGTT CTCAGGAAAC
CAGGTCTGGA GATATGATGA TACTAACCAT ATTATGGATA AAGACTATCC GAGACTAATA
GAAGAAGACT TCCCAGGAAT TGGTGATAAA GTAGATGCTG TCTATGAGAA AAATGGTTAT
ATCTATTTTT TCAACGGACC CATACAGTTT GAATACAGCA TCTGGAGTAA CCGTATTGTT
CGCGTCATGC CAGCAAATTC CATTTTGTGG TGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002418.1
CDS30..1445
Misc Feature(1)123..302(+)
Misc Feature(2)189..191(+)
Misc Feature(3)309..332(+)
Misc Feature(4)336..338(+)
Misc Feature(5)363..830(+)
Misc Feature(6)363..830(+)
Misc Feature(7)519..761(+)
Misc Feature(8)555..767(+)
Misc Feature(9)693..725(+)
Misc Feature(10)831..1442(+)
Misc Feature(11)870..1442(+)
Misc Feature(12)900..1331(+)
Misc Feature(13)1125..1127(+)
Exon (1)1..149
Gene:MMP13
Gene Synonym:
Exon (2)150..391
Gene:MMP13
Gene Synonym:
Exon (3)392..540
Gene:MMP13
Gene Synonym:
Exon (4)541..666
Gene:MMP13
Gene Synonym:
Exon (5)667..828
Gene:MMP13
Gene Synonym:
Exon (6)829..946
Gene:MMP13
Gene Synonym:
Exon (7)947..1080
Gene:MMP13
Gene Synonym:
Exon (8)1081..1240
Gene:MMP13
Gene Synonym:
Exon (9)1241..1344
Gene:MMP13
Gene Synonym:
Exon (10)1345..2719
Gene:MMP13
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_002427
Organism Homo sapiens (human)
Definition Homo sapiens matrix metallopeptidase 13 (MMP13), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002427

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCATCCAG GGGTCCTGGC TGCCTTCCTC TTCTTGAGCT GGACTCATTG TCGGGCCCTG 
CCCCTTCCCA GTGGTGGTGA TGAAGATGAT TTGTCTGAGG AAGACCTCCA GTTTGCAGAG
CGCTACCTGA GATCATACTA CCATCCTACA AATCTCGCGG GAATCCTGAA GGAGAATGCA
GCAAGCTCCA TGACTGAGAG GCTCCGAGAA ATGCAGTCTT TCTTCGGCTT AGAGGTGACT
GGCAAACTTG ACGATAACAC CTTAGATGTC ATGAAAAAGC CAAGATGCGG GGTTCCTGAT
GTGGGTGAAT ACAATGTTTT CCCTCGAACT CTTAAATGGT CCAAAATGAA TTTAACCTAC
AGAATTGTGA ATTACACCCC TGATATGACT CATTCTGAAG TCGAAAAGGC ATTCAAAAAA
GCCTTCAAAG TTTGGTCCGA TGTAACTCCT CTGAATTTTA CCAGACTTCA CGATGGCATT
GCTGACATCA TGATCTCTTT TGGAATTAAG GAGCATGGCG ACTTCTACCC ATTTGATGGG
CCCTCTGGCC TGCTGGCTCA TGCTTTTCCT CCTGGGCCAA ATTATGGAGG AGATGCCCAT
TTTGATGATG ATGAAACCTG GACAAGTAGT TCCAAAGGCT ACAACTTGTT TCTTGTTGCT
GCGCATGAGT TCGGCCACTC CTTAGGTCTT GACCACTCCA AGGACCCTGG AGCACTCATG
TTTCCTATCT ACACCTACAC CGGCAAAAGC CACTTTATGC TTCCTGATGA CGATGTACAA
GGGATCCAGT CTCTCTATGG TCCAGGAGAT GAAGACCCCA ACCCTAAACA TCCAAAAACG
CCAGACAAAT GTGACCCTTC CTTATCCCTT GATGCCATTA CCAGTCTCCG AGGAGAAACA
ATGATCTTTA AAGACAGATT CTTCTGGCGC CTGCATCCTC AGCAGGTTGA TGCGGAGCTG
TTTTTAACGA AATCATTTTG GCCAGAACTT CCCAACCGTA TTGATGCTGC ATATGAGCAC
CCTTCTCATG ACCTCATCTT CATCTTCAGA GGTAGAAAAT TTTGGGCTCT TAATGGTTAT
GACATTCTGG AAGGTTATCC CAAAAAAATA TCTGAACTGG GTCTTCCAAA AGAAGTTAAG
AAGATAAGTG CAGCTGTTCA CTTTGAGGAT ACAGGCAAGA CTCTCCTGTT CTCAGGAAAC
CAGGTCTGGA GATATGATGA TACTAACCAT ATTATGGATA AAGACTATCC GAGACTAATA
GAAGAAGACT TCCCAGGAAT TGGTGATAAA GTAGATGCTG TCTATGAGAA AAATGGTTAT
ATCTATTTTT TCAACGGACC CATACAGTTT GAATACAGCA TCTGGAGTAA CCGTATTGTT
CGCGTCATGC CAGCAAATTC CATTTTGTGG TGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Shedding of NG2 by MMP-13 attenuates anoikis
DNA Cell Biol. 33 (12), 854-862 (2014)
Joo NE, Miao D, Bermudez M, Stallcup WB and Kapila YL.


book

Common polymorphism in the MMP-13 gene may contribute to the risk of human cancers: a meta-analysis
Tumour Biol. 35 (10), 10137-10148 (2014)
Gao P, Yang JL, Zhao H, You JH and Hu Y.


book

IL-1beta-induced matrix metalloproteinase-13 is activated by a disintegrin and metalloprotease-28-regulated proliferation of human osteoblast-like cells
Exp. Cell Res. 323 (1), 165-177 (2014)
Ozeki N, Kawai R, Yamaguchi H, Hiyama T, Kinoshita K, Hase N, Nakata K, Kondo A, Mogi M and Nakamura H.


book

Immunohistochemical analysis of matrix metalloproteinase-13 in human caries dentin
Eur J Histochem 58 (1), 2318 (2014)
Loreto C, Galanti C, Musumeci G, Rusu MC and Leonardi R.


book

Association of MMP7-181A/G and MMP13-77A/G polymorphisms with colorectal cancer in a Mexican population
Genet. Mol. Res. 13 (2), 3537-3544 (2014)
Moreno-Ortiz JM, Gutierrez-Angulo M, Partida-Perez M, Peregrina-Sandoval J, Ramirez-Ramirez R, Muniz-Mendoza R, Suarez-Villanueva S, Centeno-Flores M, Maciel-Gutierrez V, Cabrales-Vazquez JE and Ayala-Madrigal ML.


book

Cellular mechanisms for human procollagenase-3 (MMP-13) activation. Evidence that MT1-MMP (MMP-14) and gelatinase a (MMP-2) are able to generate active enzyme
J. Biol. Chem. 271 (29), 17124-17131 (1996)
Knauper V, Will H, Lopez-Otin C, Smith B, Atkinson SJ, Stanton H, Hembry RM and Murphy G.


book

Cloning, expression, and type II collagenolytic activity of matrix metalloproteinase-13 from human osteoarthritic cartilage
J. Clin. Invest. 97 (3), 761-768 (1996)
Mitchell PG, Magna HA, Reeves LM, Lopresti-Morrow LL, Yocum SA, Rosner PJ, Geoghegan KF and Hambor JE.


book

Biochemical characterization of human collagenase-3
J. Biol. Chem. 271 (3), 1544-1550 (1996)
Knauper V, Lopez-Otin C, Smith B, Knight G and Murphy G.


book

The human collagenase-3 (CLG3) gene is located on chromosome 11q22.3 clustered to other members of the matrix metalloproteinase gene family
Genomics 26 (3), 615-618 (1995)
Pendas AM, Matilla T, Estivill X and Lopez-Otin C.


book

Molecular cloning and expression of collagenase-3, a novel human matrix metalloproteinase produced by breast carcinomas
J. Biol. Chem. 269 (24), 16766-16773 (1994)
Freije JM, Diez-Itza I, Balbin M, Sanchez LM, Blasco R, Tolivia J and Lopez-Otin C.


 
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