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NOS2 cDNA ORF clone, Homo sapiens (human)

Gene Symbol NOS2
Entrez Gene ID 4843
Full Name nitric oxide synthase 2, inducible
Synonyms HEP-NOS, INOS, NOS, NOS2A
General protein information
Preferred Names
nitric oxide synthase, inducible
Names
nitric oxide synthase, inducible
NOS type II
NOS, type II
inducible NOS
hepatocyte NOS
inducible NO synthase
nitric oxide synthase, macrophage
peptidyl-cysteine S-nitrosylase NOS2
nitric oxide synthase 2A (inducible, hepatocytes)
NP_000616.3
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

17

17q11.2

Summary Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

163730

Disorder Html: {Hypertension, susceptibility to}, 145500 (2); {Malaria, resistance

mRNA and Protein(s)

mRNA Protein Name
XM_011524859 XP_011523161 nitric oxide synthase, inducible isoform X1
XM_011524860 XP_011523162 nitric oxide synthase, inducible isoform X2
XM_011524861 XP_011523163 nitric oxide synthase, inducible isoform X3
XM_011524862 XP_011523164 nitric oxide synthase, inducible isoform X4
NM_000625 NP_000616 nitric oxide synthase, inducible



Homo sapiens (human) NOS2 NP_000616.3
Pan troglodytes (chimpanzee) NOS2 XP_001148238.1
Macaca mulatta (Rhesus monkey) NOS2 XP_001106245.1
Canis lupus familiaris (dog) NOS2 XP_005624903.1
Bos taurus (cattle) NOS2 NP_001070267.1
Mus musculus (house mouse) Nos2 NP_035057.1
Rattus norvegicus (Norway rat) Nos2 NP_036743.3
Rattus norvegicus (Norway rat) LOC497963 XP_006247011.1
Gallus gallus (chicken) NOS2 NP_990292.1
Danio rerio (zebrafish) nos2a NP_001098407.1
Danio rerio (zebrafish) nos2b NP_001106973.1
Xenopus (Silurana) tropicalis (western clawed frog) nos2 XP_002935342.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NOS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu28036 XM_011524859 PREDICTED: Homo sapiens nitric oxide synthase 2, inducible (NOS2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $384.30
$549.00
OHu59288 XM_011524860 PREDICTED: Homo sapiens nitric oxide synthase 2, inducible (NOS2), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00
OHu59289 XM_011524861 PREDICTED: Homo sapiens nitric oxide synthase 2, inducible (NOS2), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00
OHu59290 XM_011524862 PREDICTED: Homo sapiens nitric oxide synthase 2, inducible (NOS2), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu28036 NM_000625 Homo sapiens nitric oxide synthase 2, inducible (NOS2), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $384.30
$549.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu28036
Clone ID Related Accession (Same CDS sequence) NM_000625 , XM_011524859
Accession Version XM_011524859.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3462bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product nitric oxide synthase, inducible isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGGCCTGTC CTTGGAAATT TCTGTTCAAG ACCAAATTCC ACCAGTATGC AATGAATGGG 
GAAAAAGACA TCAACAACAA TGTGGAGAAA GCCCCCTGTG CCACCTCCAG TCCAGTGACA
CAGGATGACC TTCAGTATCA CAACCTCAGC AAGCAGCAGA ATGAGTCCCC GCAGCCCCTC
GTGGAGACGG GAAAGAAGTC TCCAGAATCT CTGGTCAAGC TGGATGCAAC CCCATTGTCC
TCCCCACGGC ATGTGAGGAT CAAAAACTGG GGCAGCGGGA TGACTTTCCA AGACACACTT
CACCATAAGG CCAAAGGGAT TTTAACTTGC AGGTCCAAAT CTTGCCTGGG GTCCATTATG
ACTCCCAAAA GTTTGACCAG AGGACCCAGG GACAAGCCTA CCCCTCCAGA TGAGCTTCTA
CCTCAAGCTA TCGAATTTGT CAACCAATAT TACGGCTCCT TCAAAGAGGC AAAAATAGAG
GAACATCTGG CCAGGGTGGA AGCGGTAACA AAGGAGATAG AAACAACAGG AACCTACCAA
CTGACGGGAG ATGAGCTCAT CTTCGCCACC AAGCAGGCCT GGCGCAATGC CCCACGCTGC
ATTGGGAGGA TCCAGTGGTC CAACCTGCAG GTCTTCGATG CCCGCAGCTG TTCCACTGCC
CGGGAAATGT TTGAACACAT CTGCAGACAC GTGCGTTACT CCACCAACAA TGGCAACATC
AGGTCGGCCA TCACCGTGTT CCCCCAGCGG AGTGATGGCA AGCACGACTT CCGGGTGTGG
AATGCTCAGC TCATCCGCTA TGCTGGCTAC CAGATGCCAG ATGGCAGCAT CAGAGGGGAC
CCTGCCAACG TGGAATTCAC TCAGCTGTGC ATCGACCTGG GCTGGAAGCC CAAGTACGGC
CGCTTCGATG TGGTCCCCCT GGTCCTGCAG GCCAATGGCC GTGACCCTGA GCTCTTCGAA
ATCCCACCTG ACCTTGTGCT TGAGGTGGCC ATGGAACATC CCAAATACGA GTGGTTTCGG
GAACTGGAGC TAAAGTGGTA CGCCCTGCCT GCAGTGGCCA ACATGCTGCT TGAGGTGGGC
GGCCTGGAGT TCCCAGGGTG CCCCTTCAAT GGCTGGTACA TGGGCACAGA GATCGGAGTC
CGGGACTTCT GTGACGTCCA GCGCTACAAC ATCCTGGAGG AAGTGGGCAG GAGAATGGGC
CTGGAAACGC ACAAGCTGGC CTCGCTCTGG AAAGACCAGG CTGTCGTTGA GATCAACATT
GCTGTGCTCC ATAGTTTCCA GAAGCAGAAT GTGACCATCA TGGACCACCA CTCGGCTGCA
GAATCCTTCA TGAAGTACAT GCAGAATGAA TACCGGTCCC GTGGGGGCTG CCCGGCAGAC
TGGATTTGGC TGGTCCCTCC CATGTCTGGG AGCATCACCC CCGTGTTTCA CCAGGAGATG
CTGAACTACG TCCTGTCCCC TTTCTACTAC TATCAGGTAG AGGCCTGGAA AACCCATGTC
TGGCAGGACG AGAAGCGGAG ACCCAAGAGA AGAGAGATTC CATTGAAAGT CTTGGTCAAA
GCTGTGCTCT TTGCCTGTAT GCTGATGCGC AAGACAATGG CGTCCCGAGT CAGAGTCACC
ATCCTCTTTG CGACAGAGAC AGGAAAATCA GAGGCGCTGG CCTGGGACCT GGGGGCCTTA
TTCAGCTGTG CCTTCAACCC CAAGGTTGTC TGCATGGATA AGTACAGGCT GAGCTGCCTG
GAGGAGGAAC GGCTGCTGTT GGTGGTGACC AGTACGTTTG GCAATGGAGA CTGCCCTGGC
AATGGAGAGA AACTGAAGAA ATCGCTCTTC ATGCTGAAAG AGCTCAACAA CAAATTCAGG
TACGCTGTGT TTGGCCTCGG CTCCAGCATG TACCCTCGGT TCTGCGCCTT TGCTCATGAC
ATTGATCAGA AGCTGTCCCA CCTGGGGGCC TCTCAGCTCA CCCCGATGGG AGAAGGGGAT
GAGCTCAGTG GGCAGGAGGA CGCCTTCCGC AGCTGGGCCG TGCAAACCTT CAAGGCAGCC
TGTGAGACGT TTGATGTCCG AGGCAAACAG CACATTCAGA TCCCCAAGCT CTACACCTCC
AATGTGACCT GGGACCCGCA CCACTACAGG CTCGTGCAGG ACTCACAGCC TTTGGACCTC
AGCAAAGCCC TCAGCAGCAT GCATGCCAAG AACGTGTTCA CCATGAGGCT CAAATCTCGG
CAGAATCTAC AAAGTCCGAC ATCCAGCCGT GCCACCATCC TGGTGGAACT CTCCTGTGAG
GATGGCCAAG GCCTGAACTA CCTGCCGGGG GAGCACCTTG GGGTTTGCCC AGGCAACCAG
CCGGCCCTGG TCCAAGGTAT CCTGGAGCGA GTGGTGGATG GCCCCACACC CCACCAGACA
GTGCGCCTGG AGGCCCTGGA TGAGAGTGGC AGCTACTGGG TCAGTGACAA GAGGCTGCCC
CCCTGCTCAC TCAGCCAGGC CCTCACCTAC TTCCTGGACA TCACCACACC CCCAACCCAG
CTGCTGCTCC AAAAGCTGGC CCAGGTGGCC ACAGAAGAGC CTGAGAGACA GAGGCTGGAG
GCCCTGTGCC AGCCCTCAGA GTACAGCAAG TGGAAGTTCA CCAACAGCCC CACATTCCTG
GAGGTGCTAG AGGAGTTCCC GTCCCTGCGG GTGTCTGCTG GCTTCCTGCT TTCCCAGCTC
CCCATTCTGA AGCCCAGGTT CTACTCCATC AGCTCCTCCC GGGATCACAC GCCCACAGAG
ATCCACCTGA CTGTGGCCGT GGTCACCTAC CACACCCGAG ATGGCCAGGG TCCCCTGCAC
CACGGCGTCT GCAGCACATG GCTCAACAGC CTGAAGCCCC AAGACCCAGT GCCCTGCTTT
GTGCGGAATG CCAGCGGCTT CCACCTCCCC GAGGATCCCT CCCATCCTTG CATCCTCATC
GGGCCTGGCA CAGGCATCGC GCCCTTCCGC AGTTTCTGGC AGCAACGGCT CCATGACTCC
CAGCACAAGG GAGTGCGGGG AGGCCGCATG ACCTTGGTGT TTGGGTGCCG CCGCCCAGAT
GAGGACCACA TCTACCAGGA GGAGATGCTG GAGATGGCCC AGAAGGGGGT GCTGCATGCG
GTGCACACAG CCTATTCCCG CCTGCCTGGC AAGCCCAAGG TCTATGTTCA GGACATCCTG
CGGCAGCAGC TGGCCAGCGA GGTGCTCCGT GTGCTCCACA AGGAGCCAGG CCACCTCTAT
GTTTGCGGGG ATGTGCGCAT GGCCCGGGAC GTGGCCCACA CCCTGAAGCA GCTGGTGGCT
GCCAAGCTGA AATTGAATGA GGAGCAGGTC GAGGACTATT TCTTTCAGCT CAAGAGCCAG
AAGCGCTATC ACGAAGATAT CTTTGGTGCT GTATTTCCTT ACGAGGCGAA GAAGGACAGG
GTGGCGGTGC AGCCCAGCAG CCTGGAGATG TCAGCGCTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011523161.1
CDS293..3754
Misc Feature(1)542..1780(+)
Misc Feature(2)560..1741(+)
Misc Feature(3)890..1765(+)
Misc Feature(4)1904..3673(+)
Misc Feature(5)1913..2308(+)
Misc Feature(6)2498..3688(+)
Misc Feature(7)2606..3244(+)
Misc Feature(8)3008..3019(+)
Misc Feature(9)3116..3154(+)
Misc Feature(10)3227..3256(+)
Misc Feature(11)3242..3544(+)
Translation

Target ORF information:

RefSeq Version XM_011524859
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens nitric oxide synthase 2, inducible (NOS2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011524859

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGGCCTGTC CTTGGAAATT TCTGTTCAAG ACCAAATTCC ACCAGTATGC AATGAATGGG 
GAAAAAGACA TCAACAACAA TGTGGAGAAA GCCCCCTGTG CCACCTCCAG TCCAGTGACA
CAGGATGACC TTCAGTATCA CAACCTCAGC AAGCAGCAGA ATGAGTCCCC GCAGCCCCTC
GTGGAGACGG GAAAGAAGTC TCCAGAATCT CTGGTCAAGC TGGATGCAAC CCCATTGTCC
TCCCCACGGC ATGTGAGGAT CAAAAACTGG GGCAGCGGGA TGACTTTCCA AGACACACTT
CACCATAAGG CCAAAGGGAT TTTAACTTGC AGGTCCAAAT CTTGCCTGGG GTCCATTATG
ACTCCCAAAA GTTTGACCAG AGGACCCAGG GACAAGCCTA CCCCTCCAGA TGAGCTTCTA
CCTCAAGCTA TCGAATTTGT CAACCAATAT TACGGCTCCT TCAAAGAGGC AAAAATAGAG
GAACATCTGG CCAGGGTGGA AGCGGTAACA AAGGAGATAG AAACAACAGG AACCTACCAA
CTGACGGGAG ATGAGCTCAT CTTCGCCACC AAGCAGGCCT GGCGCAATGC CCCACGCTGC
ATTGGGAGGA TCCAGTGGTC CAACCTGCAG GTCTTCGATG CCCGCAGCTG TTCCACTGCC
CGGGAAATGT TTGAACACAT CTGCAGACAC GTGCGTTACT CCACCAACAA TGGCAACATC
AGGTCGGCCA TCACCGTGTT CCCCCAGCGG AGTGATGGCA AGCACGACTT CCGGGTGTGG
AATGCTCAGC TCATCCGCTA TGCTGGCTAC CAGATGCCAG ATGGCAGCAT CAGAGGGGAC
CCTGCCAACG TGGAATTCAC TCAGCTGTGC ATCGACCTGG GCTGGAAGCC CAAGTACGGC
CGCTTCGATG TGGTCCCCCT GGTCCTGCAG GCCAATGGCC GTGACCCTGA GCTCTTCGAA
ATCCCACCTG ACCTTGTGCT TGAGGTGGCC ATGGAACATC CCAAATACGA GTGGTTTCGG
GAACTGGAGC TAAAGTGGTA CGCCCTGCCT GCAGTGGCCA ACATGCTGCT TGAGGTGGGC
GGCCTGGAGT TCCCAGGGTG CCCCTTCAAT GGCTGGTACA TGGGCACAGA GATCGGAGTC
CGGGACTTCT GTGACGTCCA GCGCTACAAC ATCCTGGAGG AAGTGGGCAG GAGAATGGGC
CTGGAAACGC ACAAGCTGGC CTCGCTCTGG AAAGACCAGG CTGTCGTTGA GATCAACATT
GCTGTGCTCC ATAGTTTCCA GAAGCAGAAT GTGACCATCA TGGACCACCA CTCGGCTGCA
GAATCCTTCA TGAAGTACAT GCAGAATGAA TACCGGTCCC GTGGGGGCTG CCCGGCAGAC
TGGATTTGGC TGGTCCCTCC CATGTCTGGG AGCATCACCC CCGTGTTTCA CCAGGAGATG
CTGAACTACG TCCTGTCCCC TTTCTACTAC TATCAGGTAG AGGCCTGGAA AACCCATGTC
TGGCAGGACG AGAAGCGGAG ACCCAAGAGA AGAGAGATTC CATTGAAAGT CTTGGTCAAA
GCTGTGCTCT TTGCCTGTAT GCTGATGCGC AAGACAATGG CGTCCCGAGT CAGAGTCACC
ATCCTCTTTG CGACAGAGAC AGGAAAATCA GAGGCGCTGG CCTGGGACCT GGGGGCCTTA
TTCAGCTGTG CCTTCAACCC CAAGGTTGTC TGCATGGATA AGTACAGGCT GAGCTGCCTG
GAGGAGGAAC GGCTGCTGTT GGTGGTGACC AGTACGTTTG GCAATGGAGA CTGCCCTGGC
AATGGAGAGA AACTGAAGAA ATCGCTCTTC ATGCTGAAAG AGCTCAACAA CAAATTCAGG
TACGCTGTGT TTGGCCTCGG CTCCAGCATG TACCCTCGGT TCTGCGCCTT TGCTCATGAC
ATTGATCAGA AGCTGTCCCA CCTGGGGGCC TCTCAGCTCA CCCCGATGGG AGAAGGGGAT
GAGCTCAGTG GGCAGGAGGA CGCCTTCCGC AGCTGGGCCG TGCAAACCTT CAAGGCAGCC
TGTGAGACGT TTGATGTCCG AGGCAAACAG CACATTCAGA TCCCCAAGCT CTACACCTCC
AATGTGACCT GGGACCCGCA CCACTACAGG CTCGTGCAGG ACTCACAGCC TTTGGACCTC
AGCAAAGCCC TCAGCAGCAT GCATGCCAAG AACGTGTTCA CCATGAGGCT CAAATCTCGG
CAGAATCTAC AAAGTCCGAC ATCCAGCCGT GCCACCATCC TGGTGGAACT CTCCTGTGAG
GATGGCCAAG GCCTGAACTA CCTGCCGGGG GAGCACCTTG GGGTTTGCCC AGGCAACCAG
CCGGCCCTGG TCCAAGGTAT CCTGGAGCGA GTGGTGGATG GCCCCACACC CCACCAGACA
GTGCGCCTGG AGGCCCTGGA TGAGAGTGGC AGCTACTGGG TCAGTGACAA GAGGCTGCCC
CCCTGCTCAC TCAGCCAGGC CCTCACCTAC TTCCTGGACA TCACCACACC CCCAACCCAG
CTGCTGCTCC AAAAGCTGGC CCAGGTGGCC ACAGAAGAGC CTGAGAGACA GAGGCTGGAG
GCCCTGTGCC AGCCCTCAGA GTACAGCAAG TGGAAGTTCA CCAACAGCCC CACATTCCTG
GAGGTGCTAG AGGAGTTCCC GTCCCTGCGG GTGTCTGCTG GCTTCCTGCT TTCCCAGCTC
CCCATTCTGA AGCCCAGGTT CTACTCCATC AGCTCCTCCC GGGATCACAC GCCCACAGAG
ATCCACCTGA CTGTGGCCGT GGTCACCTAC CACACCCGAG ATGGCCAGGG TCCCCTGCAC
CACGGCGTCT GCAGCACATG GCTCAACAGC CTGAAGCCCC AAGACCCAGT GCCCTGCTTT
GTGCGGAATG CCAGCGGCTT CCACCTCCCC GAGGATCCCT CCCATCCTTG CATCCTCATC
GGGCCTGGCA CAGGCATCGC GCCCTTCCGC AGTTTCTGGC AGCAACGGCT CCATGACTCC
CAGCACAAGG GAGTGCGGGG AGGCCGCATG ACCTTGGTGT TTGGGTGCCG CCGCCCAGAT
GAGGACCACA TCTACCAGGA GGAGATGCTG GAGATGGCCC AGAAGGGGGT GCTGCATGCG
GTGCACACAG CCTATTCCCG CCTGCCTGGC AAGCCCAAGG TCTATGTTCA GGACATCCTG
CGGCAGCAGC TGGCCAGCGA GGTGCTCCGT GTGCTCCACA AGGAGCCAGG CCACCTCTAT
GTTTGCGGGG ATGTGCGCAT GGCCCGGGAC GTGGCCCACA CCCTGAAGCA GCTGGTGGCT
GCCAAGCTGA AATTGAATGA GGAGCAGGTC GAGGACTATT TCTTTCAGCT CAAGAGCCAG
AAGCGCTATC ACGAAGATAT CTTTGGTGCT GTATTTCCTT ACGAGGCGAA GAAGGACAGG
GTGGCGGTGC AGCCCAGCAG CCTGGAGATG TCAGCGCTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59288
Clone ID Related Accession (Same CDS sequence) XM_011524860
Accession Version XM_011524860.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3459bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product nitric oxide synthase, inducible isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGCGCGAGG GGCTGGGCGC GGCGTGCGAG GCCGCCGGGC CCCGGCTGTG CAGGCTGCTC 
TGCAGGATCC TCCGGCCGAA GCCTGACTGC TGCCCGCGCA GAGCCAGTCC AGTGACACAG
GATGACCTTC AGTATCACAA CCTCAGCAAG CAGCAGAATG AGTCCCCGCA GCCCCTCGTG
GAGACGGGAA AGAAGTCTCC AGAATCTCTG GTCAAGCTGG ATGCAACCCC ATTGTCCTCC
CCACGGCATG TGAGGATCAA AAACTGGGGC AGCGGGATGA CTTTCCAAGA CACACTTCAC
CATAAGGCCA AAGGGATTTT AACTTGCAGG TCCAAATCTT GCCTGGGGTC CATTATGACT
CCCAAAAGTT TGACCAGAGG ACCCAGGGAC AAGCCTACCC CTCCAGATGA GCTTCTACCT
CAAGCTATCG AATTTGTCAA CCAATATTAC GGCTCCTTCA AAGAGGCAAA AATAGAGGAA
CATCTGGCCA GGGTGGAAGC GGTAACAAAG GAGATAGAAA CAACAGGAAC CTACCAACTG
ACGGGAGATG AGCTCATCTT CGCCACCAAG CAGGCCTGGC GCAATGCCCC ACGCTGCATT
GGGAGGATCC AGTGGTCCAA CCTGCAGGTC TTCGATGCCC GCAGCTGTTC CACTGCCCGG
GAAATGTTTG AACACATCTG CAGACACGTG CGTTACTCCA CCAACAATGG CAACATCAGG
TCGGCCATCA CCGTGTTCCC CCAGCGGAGT GATGGCAAGC ACGACTTCCG GGTGTGGAAT
GCTCAGCTCA TCCGCTATGC TGGCTACCAG ATGCCAGATG GCAGCATCAG AGGGGACCCT
GCCAACGTGG AATTCACTCA GCTGTGCATC GACCTGGGCT GGAAGCCCAA GTACGGCCGC
TTCGATGTGG TCCCCCTGGT CCTGCAGGCC AATGGCCGTG ACCCTGAGCT CTTCGAAATC
CCACCTGACC TTGTGCTTGA GGTGGCCATG GAACATCCCA AATACGAGTG GTTTCGGGAA
CTGGAGCTAA AGTGGTACGC CCTGCCTGCA GTGGCCAACA TGCTGCTTGA GGTGGGCGGC
CTGGAGTTCC CAGGGTGCCC CTTCAATGGC TGGTACATGG GCACAGAGAT CGGAGTCCGG
GACTTCTGTG ACGTCCAGCG CTACAACATC CTGGAGGAAG TGGGCAGGAG AATGGGCCTG
GAAACGCACA AGCTGGCCTC GCTCTGGAAA GACCAGGCTG TCGTTGAGAT CAACATTGCT
GTGCTCCATA GTTTCCAGAA GCAGAATGTG ACCATCATGG ACCACCACTC GGCTGCAGAA
TCCTTCATGA AGTACATGCA GAATGAATAC CGGTCCCGTG GGGGCTGCCC GGCAGACTGG
ATTTGGCTGG TCCCTCCCAT GTCTGGGAGC ATCACCCCCG TGTTTCACCA GGAGATGCTG
AACTACGTCC TGTCCCCTTT CTACTACTAT CAGGTAGAGG CCTGGAAAAC CCATGTCTGG
CAGGACGAGA AGCGGAGACC CAAGAGAAGA GAGATTCCAT TGAAAGTCTT GGTCAAAGCT
GTGCTCTTTG CCTGTATGCT GATGCGCAAG ACAATGGCGT CCCGAGTCAG AGTCACCATC
CTCTTTGCGA CAGAGACAGG AAAATCAGAG GCGCTGGCCT GGGACCTGGG GGCCTTATTC
AGCTGTGCCT TCAACCCCAA GGTTGTCTGC ATGGATAAGT ACAGGCTGAG CTGCCTGGAG
GAGGAACGGC TGCTGTTGGT GGTGACCAGT ACGTTTGGCA ATGGAGACTG CCCTGGCAAT
GGAGAGAAAC TGAAGAAATC GCTCTTCATG CTGAAAGAGC TCAACAACAA ATTCAGGTAC
GCTGTGTTTG GCCTCGGCTC CAGCATGTAC CCTCGGTTCT GCGCCTTTGC TCATGACATT
GATCAGAAGC TGTCCCACCT GGGGGCCTCT CAGCTCACCC CGATGGGAGA AGGGGATGAG
CTCAGTGGGC AGGAGGACGC CTTCCGCAGC TGGGCCGTGC AAACCTTCAA GGCAGCCTGT
GAGACGTTTG ATGTCCGAGG CAAACAGCAC ATTCAGATCC CCAAGCTCTA CACCTCCAAT
GTGACCTGGG ACCCGCACCA CTACAGGCTC GTGCAGGACT CACAGCCTTT GGACCTCAGC
AAAGCCCTCA GCAGCATGCA TGCCAAGAAC GTGTTCACCA TGAGGCTCAA ATCTCGGCAG
AATCTACAAA GTCCGACATC CAGCCGTGCC ACCATCCTGG TGGAACTCTC CTGTGAGGAT
GGCCAAGGCC TGAACTACCT GCCGGGGGAG CACCTTGGGG TTTGCCCAGG CAACCAGCCG
GCCCTGGTCC AAGGTATCCT GGAGCGAGTG GTGGATGGCC CCACACCCCA CCAGACAGTG
CGCCTGGAGG CCCTGGATGA GAGTGGCAGC TACTGGGTCA GTGACAAGAG GCTGCCCCCC
TGCTCACTCA GCCAGGCCCT CACCTACTTC CTGGACATCA CCACACCCCC AACCCAGCTG
CTGCTCCAAA AGCTGGCCCA GGTGGCCACA GAAGAGCCTG AGAGACAGAG GCTGGAGGCC
CTGTGCCAGC CCTCAGAGTA CAGCAAGTGG AAGTTCACCA ACAGCCCCAC ATTCCTGGAG
GTGCTAGAGG AGTTCCCGTC CCTGCGGGTG TCTGCTGGCT TCCTGCTTTC CCAGCTCCCC
ATTCTGAAGC CCAGGTTCTA CTCCATCAGC TCCTCCCGGG ATCACACGCC CACAGAGATC
CACCTGACTG TGGCCGTGGT CACCTACCAC ACCCGAGATG GCCAGGGTCC CCTGCACCAC
GGCGTCTGCA GCACATGGCT CAACAGCCTG AAGCCCCAAG ACCCAGTGCC CTGCTTTGTG
CGGAATGCCA GCGGCTTCCA CCTCCCCGAG GATCCCTCCC ATCCTTGCAT CCTCATCGGG
CCTGGCACAG GCATCGCGCC CTTCCGCAGT TTCTGGCAGC AACGGCTCCA TGACTCCCAG
CACAAGGGAG TGCGGGGAGG CCGCATGACC TTGGTGTTTG GGTGCCGCCG CCCAGATGAG
GACCACATCT ACCAGGAGGA GATGCTGGAG ATGGCCCAGA AGGGGGTGCT GCATGCGGTG
CACACAGCCT ATTCCCGCCT GCCTGGCAAG CCCAAGGTCT ATGTTCAGGA CATCCTGCGG
CAGCAGCTGG CCAGCGAGGT GCTCCGTGTG CTCCACAAGG AGCCAGGCCA CCTCTATGTT
TGCGGGGATG TGCGCATGGC CCGGGACGTG GCCCACACCC TGAAGCAGCT GGTGGCTGCC
AAGCTGAAAT TGAATGAGGA GCAGGTCGAG GACTATTTCT TTCAGCTCAA GAGCCAGAAG
CGCTATCACG AAGATATCTT TGGTGCTGTA TTTCCTTACG AGGCGAAGAA GGACAGGGTG
GCGGTGCAGC CCAGCAGCCT GGAGATGTCA GCGCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011523162.1
CDS21..3479
Misc Feature(1)267..1505(+)
Misc Feature(2)285..1466(+)
Misc Feature(3)615..1490(+)
Misc Feature(4)1629..3398(+)
Misc Feature(5)1638..2033(+)
Misc Feature(6)2223..3413(+)
Misc Feature(7)2331..2969(+)
Misc Feature(8)2733..2744(+)
Misc Feature(9)2841..2879(+)
Misc Feature(10)2952..2981(+)
Misc Feature(11)2967..3269(+)
Translation

Target ORF information:

RefSeq Version XM_011524860
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens nitric oxide synthase 2, inducible (NOS2), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011524860

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGCGCGAGG GGCTGGGCGC GGCGTGCGAG GCCGCCGGGC CCCGGCTGTG CAGGCTGCTC 
TGCAGGATCC TCCGGCCGAA GCCTGACTGC TGCCCGCGCA GAGCCAGTCC AGTGACACAG
GATGACCTTC AGTATCACAA CCTCAGCAAG CAGCAGAATG AGTCCCCGCA GCCCCTCGTG
GAGACGGGAA AGAAGTCTCC AGAATCTCTG GTCAAGCTGG ATGCAACCCC ATTGTCCTCC
CCACGGCATG TGAGGATCAA AAACTGGGGC AGCGGGATGA CTTTCCAAGA CACACTTCAC
CATAAGGCCA AAGGGATTTT AACTTGCAGG TCCAAATCTT GCCTGGGGTC CATTATGACT
CCCAAAAGTT TGACCAGAGG ACCCAGGGAC AAGCCTACCC CTCCAGATGA GCTTCTACCT
CAAGCTATCG AATTTGTCAA CCAATATTAC GGCTCCTTCA AAGAGGCAAA AATAGAGGAA
CATCTGGCCA GGGTGGAAGC GGTAACAAAG GAGATAGAAA CAACAGGAAC CTACCAACTG
ACGGGAGATG AGCTCATCTT CGCCACCAAG CAGGCCTGGC GCAATGCCCC ACGCTGCATT
GGGAGGATCC AGTGGTCCAA CCTGCAGGTC TTCGATGCCC GCAGCTGTTC CACTGCCCGG
GAAATGTTTG AACACATCTG CAGACACGTG CGTTACTCCA CCAACAATGG CAACATCAGG
TCGGCCATCA CCGTGTTCCC CCAGCGGAGT GATGGCAAGC ACGACTTCCG GGTGTGGAAT
GCTCAGCTCA TCCGCTATGC TGGCTACCAG ATGCCAGATG GCAGCATCAG AGGGGACCCT
GCCAACGTGG AATTCACTCA GCTGTGCATC GACCTGGGCT GGAAGCCCAA GTACGGCCGC
TTCGATGTGG TCCCCCTGGT CCTGCAGGCC AATGGCCGTG ACCCTGAGCT CTTCGAAATC
CCACCTGACC TTGTGCTTGA GGTGGCCATG GAACATCCCA AATACGAGTG GTTTCGGGAA
CTGGAGCTAA AGTGGTACGC CCTGCCTGCA GTGGCCAACA TGCTGCTTGA GGTGGGCGGC
CTGGAGTTCC CAGGGTGCCC CTTCAATGGC TGGTACATGG GCACAGAGAT CGGAGTCCGG
GACTTCTGTG ACGTCCAGCG CTACAACATC CTGGAGGAAG TGGGCAGGAG AATGGGCCTG
GAAACGCACA AGCTGGCCTC GCTCTGGAAA GACCAGGCTG TCGTTGAGAT CAACATTGCT
GTGCTCCATA GTTTCCAGAA GCAGAATGTG ACCATCATGG ACCACCACTC GGCTGCAGAA
TCCTTCATGA AGTACATGCA GAATGAATAC CGGTCCCGTG GGGGCTGCCC GGCAGACTGG
ATTTGGCTGG TCCCTCCCAT GTCTGGGAGC ATCACCCCCG TGTTTCACCA GGAGATGCTG
AACTACGTCC TGTCCCCTTT CTACTACTAT CAGGTAGAGG CCTGGAAAAC CCATGTCTGG
CAGGACGAGA AGCGGAGACC CAAGAGAAGA GAGATTCCAT TGAAAGTCTT GGTCAAAGCT
GTGCTCTTTG CCTGTATGCT GATGCGCAAG ACAATGGCGT CCCGAGTCAG AGTCACCATC
CTCTTTGCGA CAGAGACAGG AAAATCAGAG GCGCTGGCCT GGGACCTGGG GGCCTTATTC
AGCTGTGCCT TCAACCCCAA GGTTGTCTGC ATGGATAAGT ACAGGCTGAG CTGCCTGGAG
GAGGAACGGC TGCTGTTGGT GGTGACCAGT ACGTTTGGCA ATGGAGACTG CCCTGGCAAT
GGAGAGAAAC TGAAGAAATC GCTCTTCATG CTGAAAGAGC TCAACAACAA ATTCAGGTAC
GCTGTGTTTG GCCTCGGCTC CAGCATGTAC CCTCGGTTCT GCGCCTTTGC TCATGACATT
GATCAGAAGC TGTCCCACCT GGGGGCCTCT CAGCTCACCC CGATGGGAGA AGGGGATGAG
CTCAGTGGGC AGGAGGACGC CTTCCGCAGC TGGGCCGTGC AAACCTTCAA GGCAGCCTGT
GAGACGTTTG ATGTCCGAGG CAAACAGCAC ATTCAGATCC CCAAGCTCTA CACCTCCAAT
GTGACCTGGG ACCCGCACCA CTACAGGCTC GTGCAGGACT CACAGCCTTT GGACCTCAGC
AAAGCCCTCA GCAGCATGCA TGCCAAGAAC GTGTTCACCA TGAGGCTCAA ATCTCGGCAG
AATCTACAAA GTCCGACATC CAGCCGTGCC ACCATCCTGG TGGAACTCTC CTGTGAGGAT
GGCCAAGGCC TGAACTACCT GCCGGGGGAG CACCTTGGGG TTTGCCCAGG CAACCAGCCG
GCCCTGGTCC AAGGTATCCT GGAGCGAGTG GTGGATGGCC CCACACCCCA CCAGACAGTG
CGCCTGGAGG CCCTGGATGA GAGTGGCAGC TACTGGGTCA GTGACAAGAG GCTGCCCCCC
TGCTCACTCA GCCAGGCCCT CACCTACTTC CTGGACATCA CCACACCCCC AACCCAGCTG
CTGCTCCAAA AGCTGGCCCA GGTGGCCACA GAAGAGCCTG AGAGACAGAG GCTGGAGGCC
CTGTGCCAGC CCTCAGAGTA CAGCAAGTGG AAGTTCACCA ACAGCCCCAC ATTCCTGGAG
GTGCTAGAGG AGTTCCCGTC CCTGCGGGTG TCTGCTGGCT TCCTGCTTTC CCAGCTCCCC
ATTCTGAAGC CCAGGTTCTA CTCCATCAGC TCCTCCCGGG ATCACACGCC CACAGAGATC
CACCTGACTG TGGCCGTGGT CACCTACCAC ACCCGAGATG GCCAGGGTCC CCTGCACCAC
GGCGTCTGCA GCACATGGCT CAACAGCCTG AAGCCCCAAG ACCCAGTGCC CTGCTTTGTG
CGGAATGCCA GCGGCTTCCA CCTCCCCGAG GATCCCTCCC ATCCTTGCAT CCTCATCGGG
CCTGGCACAG GCATCGCGCC CTTCCGCAGT TTCTGGCAGC AACGGCTCCA TGACTCCCAG
CACAAGGGAG TGCGGGGAGG CCGCATGACC TTGGTGTTTG GGTGCCGCCG CCCAGATGAG
GACCACATCT ACCAGGAGGA GATGCTGGAG ATGGCCCAGA AGGGGGTGCT GCATGCGGTG
CACACAGCCT ATTCCCGCCT GCCTGGCAAG CCCAAGGTCT ATGTTCAGGA CATCCTGCGG
CAGCAGCTGG CCAGCGAGGT GCTCCGTGTG CTCCACAAGG AGCCAGGCCA CCTCTATGTT
TGCGGGGATG TGCGCATGGC CCGGGACGTG GCCCACACCC TGAAGCAGCT GGTGGCTGCC
AAGCTGAAAT TGAATGAGGA GCAGGTCGAG GACTATTTCT TTCAGCTCAA GAGCCAGAAG
CGCTATCACG AAGATATCTT TGGTGCTGTA TTTCCTTACG AGGCGAAGAA GGACAGGGTG
GCGGTGCAGC CCAGCAGCCT GGAGATGTCA GCGCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59289
Clone ID Related Accession (Same CDS sequence) XM_011524861
Accession Version XM_011524861.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3390bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product nitric oxide synthase, inducible isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGGCCTGTC CTTGGAAATT TCTGTTCAAG ACCAAATTCC ACCAGTATGC AATGAATGGG 
GAAAAAGACA TCAACAACAA TGTGGAGAAA GCCCCCTGTG CCACCTCCAG TCCAGTGACA
CAGGATGACC TTCAGTATCA CAACCTCAGC AAGCAGCAGA ATGAGTCCCC GCAGCCCCTC
GTGGAGACGG GAAAGAAGTC TCCAGAATCT CTGGTCAAGC TGGATGCAAC CCCATTGTCC
TCCCCACGGC ATGTGAGGAT CAAAAACTGG GGCAGCGGGA TGACTTTCCA AGACACACTT
CACCATAAGG CCAAAGGGAT TTTAACTTGC AGGTCCAAAT CTTGCCTGGG GTCCATTATG
ACTCCCAAAA GTTTGACCAG AGGACCCAGG GACAAGCCTA CCCCTCCAGA TGAGCTTCTA
CCTCAAGCTA TCGAATTTGT CAACCAATAT TACGGCTCCT TCAAAGAGGC AAAAATAGAG
GAACATCTGG CCAGGGTGGA AGCGGTAACA AAGGAGATAG AAACAACAGG AACCTACCAA
CTGACGGGAG ATGAGCTCAT CTTCGCCACC AAGCAGGCCT GGCGCAATGC CCCACGCTGC
ATTGGGAGGA TCCAGTGGTC CAACCTGCAG GTCTTCGATG CCCGCAGCTG TTCCACTGCC
CGGGAAATGT TTGAACACAT CTGCAGACAC GTGCGTTACT CCACCAACAA TGGCAACATC
AGGTCGGCCA TCACCGTGTT CCCCCAGCGG AGTGATGGCA AGCACGACTT CCGGGTGTGG
AATGCTCAGC TCATCCGCTA TGCTGGCTAC CAGATGCCAG ATGGCAGCAT CAGAGGGGAC
CCTGCCAACG TGGAATTCAC TCAGCTGTGC ATCGACCTGG GCTGGAAGCC CAAGTACGGC
CGCTTCGATG TGGTCCCCCT GGTCCTGCAG GCCAATGGCC GTGACCCTGA GCTCTTCGAA
ATCCCACCTG ACCTTGTGCT TGAGGTGGCC ATGGAACATC CCAAATACGA GTGGTTTCGG
GAACTGGAGC TAAAGTGGTA CGCCCTGCCT GCAGTGGCCA ACATGCTGCT TGAGGTGGGC
GGCCTGGAGT TCCCAGGGTG CCCCTTCAAT GGCTGGTACA TGGGCACAGA GATCGGAGTC
CGGGACTTCT GTGACGTCCA GCGCTACAAC ATCCTGGAGG AAGTGGGCAG GAGAATGGGC
CTGGAAACGC ACAAGCTGGC CTCGCTCTGG AAAGACCAGG CTGTCGTTGA GATCAACATT
GCTGTGCTCC ATAGTTTCCA GAAGCAGAAT GTGACCATCA TGGACCACCA CTCGGCTGCA
GAATCCTTCA TGAAGTACAT GCAGAATGAA TACCGGTCCC GTGGGGGCTG CCCGGCAGAC
TGGATTTGGC TGGTCCCTCC CATGTCTGGG AGCATCACCC CCGTGTTTCA CCAGGAGATG
CTGAACTACG TCCTGTCCCC TTTCTACTAC TATCAGGTAG AGGCCTGGAA AACCCATGTC
TGGCAGGACG AGAAGCGGAG ACCCAAGAGA AGAGAGATTC CATTGAAAGT CTTGGTCAAA
GCTGTGCTCT TTGCCTGTAT GCTGATGCGC AAGACAATGG CGTCCCGAGT CAGAGTCACC
ATCCTCTTTG CGACAGAGAC AGGAAAATCA GAGGCGCTGG CCTGGGACCT GGGGGCCTTA
TTCAGCTGTG CCTTCAACCC CAAGGTTGTC TGCATGGATA AGTACAGGCT GAGCTGCCTG
GAGGAGGAAC GGCTGCTGTT GGTGGTGACC AGTACGTTTG GCAATGGAGA CTGCCCTGGC
AATGGAGAGA AACTGAAGAA ATCGCTCTTC ATGCTGAAAG AGCTCAACAA CAAATTCAGG
TACGCTGTGT TTGGCCTCGG CTCCAGCATG TACCCTCGGT TCTGCGCCTT TGCTCATGAC
ATTGATCAGA AGCTGTCCCA CCTGGGGGCC TCTCAGCTCA CCCCGATGGG AGAAGGGGAT
GAGCTCAGTG GGCAGGAGGA CGCCTTCCGC AGCTGGGCCG TGCAAACCTT CAAGGCAGCC
TGTGAGACGT TTGATGTCCG AGGCAAACAG CACATTCAGA TCCCCAAGCT CTACACCTCC
AATGTGACCT GGGACCCGCA CCACTACAGG CTCGTGCAGG ACTCACAGCC TTTGGACCTC
AGCAAAGCCC TCAGCAGCAT GCATGCCAAG AACGTGTTCA CCATGAGGCT CAAATCTCGG
CAGAATCTAC AAAGTCCGAC ATCCAGCCGT GCCACCATCC TGGTGGAACT CTCCTGTGAG
GATGGCCAAG GCCTGAACTA CCTGCCGGGG GAGCACCTTG GGGTTTGCCC AGGCAACCAG
CCGGCCCTGG TCCAAGGCAG CTACTGGGTC AGTGACAAGA GGCTGCCCCC CTGCTCACTC
AGCCAGGCCC TCACCTACTT CCTGGACATC ACCACACCCC CAACCCAGCT GCTGCTCCAA
AAGCTGGCCC AGGTGGCCAC AGAAGAGCCT GAGAGACAGA GGCTGGAGGC CCTGTGCCAG
CCCTCAGAGT ACAGCAAGTG GAAGTTCACC AACAGCCCCA CATTCCTGGA GGTGCTAGAG
GAGTTCCCGT CCCTGCGGGT GTCTGCTGGC TTCCTGCTTT CCCAGCTCCC CATTCTGAAG
CCCAGGTTCT ACTCCATCAG CTCCTCCCGG GATCACACGC CCACAGAGAT CCACCTGACT
GTGGCCGTGG TCACCTACCA CACCCGAGAT GGCCAGGGTC CCCTGCACCA CGGCGTCTGC
AGCACATGGC TCAACAGCCT GAAGCCCCAA GACCCAGTGC CCTGCTTTGT GCGGAATGCC
AGCGGCTTCC ACCTCCCCGA GGATCCCTCC CATCCTTGCA TCCTCATCGG GCCTGGCACA
GGCATCGCGC CCTTCCGCAG TTTCTGGCAG CAACGGCTCC ATGACTCCCA GCACAAGGGA
GTGCGGGGAG GCCGCATGAC CTTGGTGTTT GGGTGCCGCC GCCCAGATGA GGACCACATC
TACCAGGAGG AGATGCTGGA GATGGCCCAG AAGGGGGTGC TGCATGCGGT GCACACAGCC
TATTCCCGCC TGCCTGGCAA GCCCAAGGTC TATGTTCAGG ACATCCTGCG GCAGCAGCTG
GCCAGCGAGG TGCTCCGTGT GCTCCACAAG GAGCCAGGCC ACCTCTATGT TTGCGGGGAT
GTGCGCATGG CCCGGGACGT GGCCCACACC CTGAAGCAGC TGGTGGCTGC CAAGCTGAAA
TTGAATGAGG AGCAGGTCGA GGACTATTTC TTTCAGCTCA AGAGCCAGAA GCGCTATCAC
GAAGATATCT TTGGTGCTGT ATTTCCTTAC GAGGCGAAGA AGGACAGGGT GGCGGTGCAG
CCCAGCAGCC TGGAGATGTC AGCGCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011523163.1
CDS263..3652
Misc Feature(1)512..1750(+)
Misc Feature(2)530..1711(+)
Misc Feature(3)860..1735(+)
Misc Feature(4)1874..3571(+)
Misc Feature(5)1883..2278(+)
Misc Feature(6)2468..3586(+)
Misc Feature(7)2576..3142(+)
Misc Feature(8)2906..2917(+)
Misc Feature(9)3014..3052(+)
Misc Feature(10)3125..3154(+)
Misc Feature(11)3140..3442(+)
Translation

Target ORF information:

RefSeq Version XM_011524861
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens nitric oxide synthase 2, inducible (NOS2), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011524861

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGGCCTGTC CTTGGAAATT TCTGTTCAAG ACCAAATTCC ACCAGTATGC AATGAATGGG 
GAAAAAGACA TCAACAACAA TGTGGAGAAA GCCCCCTGTG CCACCTCCAG TCCAGTGACA
CAGGATGACC TTCAGTATCA CAACCTCAGC AAGCAGCAGA ATGAGTCCCC GCAGCCCCTC
GTGGAGACGG GAAAGAAGTC TCCAGAATCT CTGGTCAAGC TGGATGCAAC CCCATTGTCC
TCCCCACGGC ATGTGAGGAT CAAAAACTGG GGCAGCGGGA TGACTTTCCA AGACACACTT
CACCATAAGG CCAAAGGGAT TTTAACTTGC AGGTCCAAAT CTTGCCTGGG GTCCATTATG
ACTCCCAAAA GTTTGACCAG AGGACCCAGG GACAAGCCTA CCCCTCCAGA TGAGCTTCTA
CCTCAAGCTA TCGAATTTGT CAACCAATAT TACGGCTCCT TCAAAGAGGC AAAAATAGAG
GAACATCTGG CCAGGGTGGA AGCGGTAACA AAGGAGATAG AAACAACAGG AACCTACCAA
CTGACGGGAG ATGAGCTCAT CTTCGCCACC AAGCAGGCCT GGCGCAATGC CCCACGCTGC
ATTGGGAGGA TCCAGTGGTC CAACCTGCAG GTCTTCGATG CCCGCAGCTG TTCCACTGCC
CGGGAAATGT TTGAACACAT CTGCAGACAC GTGCGTTACT CCACCAACAA TGGCAACATC
AGGTCGGCCA TCACCGTGTT CCCCCAGCGG AGTGATGGCA AGCACGACTT CCGGGTGTGG
AATGCTCAGC TCATCCGCTA TGCTGGCTAC CAGATGCCAG ATGGCAGCAT CAGAGGGGAC
CCTGCCAACG TGGAATTCAC TCAGCTGTGC ATCGACCTGG GCTGGAAGCC CAAGTACGGC
CGCTTCGATG TGGTCCCCCT GGTCCTGCAG GCCAATGGCC GTGACCCTGA GCTCTTCGAA
ATCCCACCTG ACCTTGTGCT TGAGGTGGCC ATGGAACATC CCAAATACGA GTGGTTTCGG
GAACTGGAGC TAAAGTGGTA CGCCCTGCCT GCAGTGGCCA ACATGCTGCT TGAGGTGGGC
GGCCTGGAGT TCCCAGGGTG CCCCTTCAAT GGCTGGTACA TGGGCACAGA GATCGGAGTC
CGGGACTTCT GTGACGTCCA GCGCTACAAC ATCCTGGAGG AAGTGGGCAG GAGAATGGGC
CTGGAAACGC ACAAGCTGGC CTCGCTCTGG AAAGACCAGG CTGTCGTTGA GATCAACATT
GCTGTGCTCC ATAGTTTCCA GAAGCAGAAT GTGACCATCA TGGACCACCA CTCGGCTGCA
GAATCCTTCA TGAAGTACAT GCAGAATGAA TACCGGTCCC GTGGGGGCTG CCCGGCAGAC
TGGATTTGGC TGGTCCCTCC CATGTCTGGG AGCATCACCC CCGTGTTTCA CCAGGAGATG
CTGAACTACG TCCTGTCCCC TTTCTACTAC TATCAGGTAG AGGCCTGGAA AACCCATGTC
TGGCAGGACG AGAAGCGGAG ACCCAAGAGA AGAGAGATTC CATTGAAAGT CTTGGTCAAA
GCTGTGCTCT TTGCCTGTAT GCTGATGCGC AAGACAATGG CGTCCCGAGT CAGAGTCACC
ATCCTCTTTG CGACAGAGAC AGGAAAATCA GAGGCGCTGG CCTGGGACCT GGGGGCCTTA
TTCAGCTGTG CCTTCAACCC CAAGGTTGTC TGCATGGATA AGTACAGGCT GAGCTGCCTG
GAGGAGGAAC GGCTGCTGTT GGTGGTGACC AGTACGTTTG GCAATGGAGA CTGCCCTGGC
AATGGAGAGA AACTGAAGAA ATCGCTCTTC ATGCTGAAAG AGCTCAACAA CAAATTCAGG
TACGCTGTGT TTGGCCTCGG CTCCAGCATG TACCCTCGGT TCTGCGCCTT TGCTCATGAC
ATTGATCAGA AGCTGTCCCA CCTGGGGGCC TCTCAGCTCA CCCCGATGGG AGAAGGGGAT
GAGCTCAGTG GGCAGGAGGA CGCCTTCCGC AGCTGGGCCG TGCAAACCTT CAAGGCAGCC
TGTGAGACGT TTGATGTCCG AGGCAAACAG CACATTCAGA TCCCCAAGCT CTACACCTCC
AATGTGACCT GGGACCCGCA CCACTACAGG CTCGTGCAGG ACTCACAGCC TTTGGACCTC
AGCAAAGCCC TCAGCAGCAT GCATGCCAAG AACGTGTTCA CCATGAGGCT CAAATCTCGG
CAGAATCTAC AAAGTCCGAC ATCCAGCCGT GCCACCATCC TGGTGGAACT CTCCTGTGAG
GATGGCCAAG GCCTGAACTA CCTGCCGGGG GAGCACCTTG GGGTTTGCCC AGGCAACCAG
CCGGCCCTGG TCCAAGGCAG CTACTGGGTC AGTGACAAGA GGCTGCCCCC CTGCTCACTC
AGCCAGGCCC TCACCTACTT CCTGGACATC ACCACACCCC CAACCCAGCT GCTGCTCCAA
AAGCTGGCCC AGGTGGCCAC AGAAGAGCCT GAGAGACAGA GGCTGGAGGC CCTGTGCCAG
CCCTCAGAGT ACAGCAAGTG GAAGTTCACC AACAGCCCCA CATTCCTGGA GGTGCTAGAG
GAGTTCCCGT CCCTGCGGGT GTCTGCTGGC TTCCTGCTTT CCCAGCTCCC CATTCTGAAG
CCCAGGTTCT ACTCCATCAG CTCCTCCCGG GATCACACGC CCACAGAGAT CCACCTGACT
GTGGCCGTGG TCACCTACCA CACCCGAGAT GGCCAGGGTC CCCTGCACCA CGGCGTCTGC
AGCACATGGC TCAACAGCCT GAAGCCCCAA GACCCAGTGC CCTGCTTTGT GCGGAATGCC
AGCGGCTTCC ACCTCCCCGA GGATCCCTCC CATCCTTGCA TCCTCATCGG GCCTGGCACA
GGCATCGCGC CCTTCCGCAG TTTCTGGCAG CAACGGCTCC ATGACTCCCA GCACAAGGGA
GTGCGGGGAG GCCGCATGAC CTTGGTGTTT GGGTGCCGCC GCCCAGATGA GGACCACATC
TACCAGGAGG AGATGCTGGA GATGGCCCAG AAGGGGGTGC TGCATGCGGT GCACACAGCC
TATTCCCGCC TGCCTGGCAA GCCCAAGGTC TATGTTCAGG ACATCCTGCG GCAGCAGCTG
GCCAGCGAGG TGCTCCGTGT GCTCCACAAG GAGCCAGGCC ACCTCTATGT TTGCGGGGAT
GTGCGCATGG CCCGGGACGT GGCCCACACC CTGAAGCAGC TGGTGGCTGC CAAGCTGAAA
TTGAATGAGG AGCAGGTCGA GGACTATTTC TTTCAGCTCA AGAGCCAGAA GCGCTATCAC
GAAGATATCT TTGGTGCTGT ATTTCCTTAC GAGGCGAAGA AGGACAGGGT GGCGGTGCAG
CCCAGCAGCC TGGAGATGTC AGCGCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59290
Clone ID Related Accession (Same CDS sequence) XM_011524862
Accession Version XM_011524862.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2796bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product nitric oxide synthase, inducible isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
ATGTTTGAAC ACATCTGCAG ACACGTGCGT TACTCCACCA ACAATGGCAA CATCAGGTCG 
GCCATCACCG TGTTCCCCCA GCGGAGTGAT GGCAAGCACG ACTTCCGGGT GTGGAATGCT
CAGCTCATCC GCTATGCTGG CTACCAGATG CCAGATGGCA GCATCAGAGG GGACCCTGCC
AACGTGGAAT TCACTCAGCT GTGCATCGAC CTGGGCTGGA AGCCCAAGTA CGGCCGCTTC
GATGTGGTCC CCCTGGTCCT GCAGGCCAAT GGCCGTGACC CTGAGCTCTT CGAAATCCCA
CCTGACCTTG TGCTTGAGGT GGCCATGGAA CATCCCAAAT ACGAGTGGTT TCGGGAACTG
GAGCTAAAGT GGTACGCCCT GCCTGCAGTG GCCAACATGC TGCTTGAGGT GGGCGGCCTG
GAGTTCCCAG GGTGCCCCTT CAATGGCTGG TACATGGGCA CAGAGATCGG AGTCCGGGAC
TTCTGTGACG TCCAGCGCTA CAACATCCTG GAGGAAGTGG GCAGGAGAAT GGGCCTGGAA
ACGCACAAGC TGGCCTCGCT CTGGAAAGAC CAGGCTGTCG TTGAGATCAA CATTGCTGTG
CTCCATAGTT TCCAGAAGCA GAATGTGACC ATCATGGACC ACCACTCGGC TGCAGAATCC
TTCATGAAGT ACATGCAGAA TGAATACCGG TCCCGTGGGG GCTGCCCGGC AGACTGGATT
TGGCTGGTCC CTCCCATGTC TGGGAGCATC ACCCCCGTGT TTCACCAGGA GATGCTGAAC
TACGTCCTGT CCCCTTTCTA CTACTATCAG GTAGAGGCCT GGAAAACCCA TGTCTGGCAG
GACGAGAAGC GGAGACCCAA GAGAAGAGAG ATTCCATTGA AAGTCTTGGT CAAAGCTGTG
CTCTTTGCCT GTATGCTGAT GCGCAAGACA ATGGCGTCCC GAGTCAGAGT CACCATCCTC
TTTGCGACAG AGACAGGAAA ATCAGAGGCG CTGGCCTGGG ACCTGGGGGC CTTATTCAGC
TGTGCCTTCA ACCCCAAGGT TGTCTGCATG GATAAGTACA GGCTGAGCTG CCTGGAGGAG
GAACGGCTGC TGTTGGTGGT GACCAGTACG TTTGGCAATG GAGACTGCCC TGGCAATGGA
GAGAAACTGA AGAAATCGCT CTTCATGCTG AAAGAGCTCA ACAACAAATT CAGGTACGCT
GTGTTTGGCC TCGGCTCCAG CATGTACCCT CGGTTCTGCG CCTTTGCTCA TGACATTGAT
CAGAAGCTGT CCCACCTGGG GGCCTCTCAG CTCACCCCGA TGGGAGAAGG GGATGAGCTC
AGTGGGCAGG AGGACGCCTT CCGCAGCTGG GCCGTGCAAA CCTTCAAGGC AGCCTGTGAG
ACGTTTGATG TCCGAGGCAA ACAGCACATT CAGATCCCCA AGCTCTACAC CTCCAATGTG
ACCTGGGACC CGCACCACTA CAGGCTCGTG CAGGACTCAC AGCCTTTGGA CCTCAGCAAA
GCCCTCAGCA GCATGCATGC CAAGAACGTG TTCACCATGA GGCTCAAATC TCGGCAGAAT
CTACAAAGTC CGACATCCAG CCGTGCCACC ATCCTGGTGG AACTCTCCTG TGAGGATGGC
CAAGGCCTGA ACTACCTGCC GGGGGAGCAC CTTGGGGTTT GCCCAGGCAA CCAGCCGGCC
CTGGTCCAAG GTATCCTGGA GCGAGTGGTG GATGGCCCCA CACCCCACCA GACAGTGCGC
CTGGAGGCCC TGGATGAGAG TGGCAGCTAC TGGGTCAGTG ACAAGAGGCT GCCCCCCTGC
TCACTCAGCC AGGCCCTCAC CTACTTCCTG GACATCACCA CACCCCCAAC CCAGCTGCTG
CTCCAAAAGC TGGCCCAGGT GGCCACAGAA GAGCCTGAGA GACAGAGGCT GGAGGCCCTG
TGCCAGCCCT CAGAGTACAG CAAGTGGAAG TTCACCAACA GCCCCACATT CCTGGAGGTG
CTAGAGGAGT TCCCGTCCCT GCGGGTGTCT GCTGGCTTCC TGCTTTCCCA GCTCCCCATT
CTGAAGCCCA GGTTCTACTC CATCAGCTCC TCCCGGGATC ACACGCCCAC AGAGATCCAC
CTGACTGTGG CCGTGGTCAC CTACCACACC CGAGATGGCC AGGGTCCCCT GCACCACGGC
GTCTGCAGCA CATGGCTCAA CAGCCTGAAG CCCCAAGACC CAGTGCCCTG CTTTGTGCGG
AATGCCAGCG GCTTCCACCT CCCCGAGGAT CCCTCCCATC CTTGCATCCT CATCGGGCCT
GGCACAGGCA TCGCGCCCTT CCGCAGTTTC TGGCAGCAAC GGCTCCATGA CTCCCAGCAC
AAGGGAGTGC GGGGAGGCCG CATGACCTTG GTGTTTGGGT GCCGCCGCCC AGATGAGGAC
CACATCTACC AGGAGGAGAT GCTGGAGATG GCCCAGAAGG GGGTGCTGCA TGCGGTGCAC
ACAGCCTATT CCCGCCTGCC TGGCAAGCCC AAGGTCTATG TTCAGGACAT CCTGCGGCAG
CAGCTGGCCA GCGAGGTGCT CCGTGTGCTC CACAAGGAGC CAGGCCACCT CTATGTTTGC
GGGGATGTGC GCATGGCCCG GGACGTGGCC CACACCCTGA AGCAGCTGGT GGCTGCCAAG
CTGAAATTGA ATGAGGAGCA GGTCGAGGAC TATTTCTTTC AGCTCAAGAG CCAGAAGCGC
TATCACGAAG ATATCTTTGG TGCTGTATTT CCTTACGAGG CGAAGAAGGA CAGGGTGGCG
GTGCAGCCCA GCAGCCTGGA GATGTCAGCG CTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011523164.1
CDS528..3323
Misc Feature(1)528..1349(+)
Misc Feature(2)648..1334(+)
Misc Feature(3)1014..1310(+)
Misc Feature(4)1473..3242(+)
Misc Feature(5)1482..1877(+)
Misc Feature(6)2067..3257(+)
Misc Feature(7)2175..2813(+)
Misc Feature(8)2577..2588(+)
Misc Feature(9)2685..2723(+)
Misc Feature(10)2796..2825(+)
Misc Feature(11)2811..3113(+)
Translation

Target ORF information:

RefSeq Version XM_011524862
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens nitric oxide synthase 2, inducible (NOS2), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011524862

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
ATGTTTGAAC ACATCTGCAG ACACGTGCGT TACTCCACCA ACAATGGCAA CATCAGGTCG 
GCCATCACCG TGTTCCCCCA GCGGAGTGAT GGCAAGCACG ACTTCCGGGT GTGGAATGCT
CAGCTCATCC GCTATGCTGG CTACCAGATG CCAGATGGCA GCATCAGAGG GGACCCTGCC
AACGTGGAAT TCACTCAGCT GTGCATCGAC CTGGGCTGGA AGCCCAAGTA CGGCCGCTTC
GATGTGGTCC CCCTGGTCCT GCAGGCCAAT GGCCGTGACC CTGAGCTCTT CGAAATCCCA
CCTGACCTTG TGCTTGAGGT GGCCATGGAA CATCCCAAAT ACGAGTGGTT TCGGGAACTG
GAGCTAAAGT GGTACGCCCT GCCTGCAGTG GCCAACATGC TGCTTGAGGT GGGCGGCCTG
GAGTTCCCAG GGTGCCCCTT CAATGGCTGG TACATGGGCA CAGAGATCGG AGTCCGGGAC
TTCTGTGACG TCCAGCGCTA CAACATCCTG GAGGAAGTGG GCAGGAGAAT GGGCCTGGAA
ACGCACAAGC TGGCCTCGCT CTGGAAAGAC CAGGCTGTCG TTGAGATCAA CATTGCTGTG
CTCCATAGTT TCCAGAAGCA GAATGTGACC ATCATGGACC ACCACTCGGC TGCAGAATCC
TTCATGAAGT ACATGCAGAA TGAATACCGG TCCCGTGGGG GCTGCCCGGC AGACTGGATT
TGGCTGGTCC CTCCCATGTC TGGGAGCATC ACCCCCGTGT TTCACCAGGA GATGCTGAAC
TACGTCCTGT CCCCTTTCTA CTACTATCAG GTAGAGGCCT GGAAAACCCA TGTCTGGCAG
GACGAGAAGC GGAGACCCAA GAGAAGAGAG ATTCCATTGA AAGTCTTGGT CAAAGCTGTG
CTCTTTGCCT GTATGCTGAT GCGCAAGACA ATGGCGTCCC GAGTCAGAGT CACCATCCTC
TTTGCGACAG AGACAGGAAA ATCAGAGGCG CTGGCCTGGG ACCTGGGGGC CTTATTCAGC
TGTGCCTTCA ACCCCAAGGT TGTCTGCATG GATAAGTACA GGCTGAGCTG CCTGGAGGAG
GAACGGCTGC TGTTGGTGGT GACCAGTACG TTTGGCAATG GAGACTGCCC TGGCAATGGA
GAGAAACTGA AGAAATCGCT CTTCATGCTG AAAGAGCTCA ACAACAAATT CAGGTACGCT
GTGTTTGGCC TCGGCTCCAG CATGTACCCT CGGTTCTGCG CCTTTGCTCA TGACATTGAT
CAGAAGCTGT CCCACCTGGG GGCCTCTCAG CTCACCCCGA TGGGAGAAGG GGATGAGCTC
AGTGGGCAGG AGGACGCCTT CCGCAGCTGG GCCGTGCAAA CCTTCAAGGC AGCCTGTGAG
ACGTTTGATG TCCGAGGCAA ACAGCACATT CAGATCCCCA AGCTCTACAC CTCCAATGTG
ACCTGGGACC CGCACCACTA CAGGCTCGTG CAGGACTCAC AGCCTTTGGA CCTCAGCAAA
GCCCTCAGCA GCATGCATGC CAAGAACGTG TTCACCATGA GGCTCAAATC TCGGCAGAAT
CTACAAAGTC CGACATCCAG CCGTGCCACC ATCCTGGTGG AACTCTCCTG TGAGGATGGC
CAAGGCCTGA ACTACCTGCC GGGGGAGCAC CTTGGGGTTT GCCCAGGCAA CCAGCCGGCC
CTGGTCCAAG GTATCCTGGA GCGAGTGGTG GATGGCCCCA CACCCCACCA GACAGTGCGC
CTGGAGGCCC TGGATGAGAG TGGCAGCTAC TGGGTCAGTG ACAAGAGGCT GCCCCCCTGC
TCACTCAGCC AGGCCCTCAC CTACTTCCTG GACATCACCA CACCCCCAAC CCAGCTGCTG
CTCCAAAAGC TGGCCCAGGT GGCCACAGAA GAGCCTGAGA GACAGAGGCT GGAGGCCCTG
TGCCAGCCCT CAGAGTACAG CAAGTGGAAG TTCACCAACA GCCCCACATT CCTGGAGGTG
CTAGAGGAGT TCCCGTCCCT GCGGGTGTCT GCTGGCTTCC TGCTTTCCCA GCTCCCCATT
CTGAAGCCCA GGTTCTACTC CATCAGCTCC TCCCGGGATC ACACGCCCAC AGAGATCCAC
CTGACTGTGG CCGTGGTCAC CTACCACACC CGAGATGGCC AGGGTCCCCT GCACCACGGC
GTCTGCAGCA CATGGCTCAA CAGCCTGAAG CCCCAAGACC CAGTGCCCTG CTTTGTGCGG
AATGCCAGCG GCTTCCACCT CCCCGAGGAT CCCTCCCATC CTTGCATCCT CATCGGGCCT
GGCACAGGCA TCGCGCCCTT CCGCAGTTTC TGGCAGCAAC GGCTCCATGA CTCCCAGCAC
AAGGGAGTGC GGGGAGGCCG CATGACCTTG GTGTTTGGGT GCCGCCGCCC AGATGAGGAC
CACATCTACC AGGAGGAGAT GCTGGAGATG GCCCAGAAGG GGGTGCTGCA TGCGGTGCAC
ACAGCCTATT CCCGCCTGCC TGGCAAGCCC AAGGTCTATG TTCAGGACAT CCTGCGGCAG
CAGCTGGCCA GCGAGGTGCT CCGTGTGCTC CACAAGGAGC CAGGCCACCT CTATGTTTGC
GGGGATGTGC GCATGGCCCG GGACGTGGCC CACACCCTGA AGCAGCTGGT GGCTGCCAAG
CTGAAATTGA ATGAGGAGCA GGTCGAGGAC TATTTCTTTC AGCTCAAGAG CCAGAAGCGC
TATCACGAAG ATATCTTTGG TGCTGTATTT CCTTACGAGG CGAAGAAGGA CAGGGTGGCG
GTGCAGCCCA GCAGCCTGGA GATGTCAGCG CTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28036
Clone ID Related Accession (Same CDS sequence) NM_000625 , XM_011524859
Accession Version NM_000625.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3462bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product nitric oxide synthase, inducible
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC130289.11. This sequence is a reference standard in the RefSeqGene project. On Sep 25, 2008 this sequence version replaced gi:24041028. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U31511.1, X73029.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGGCCTGTC CTTGGAAATT TCTGTTCAAG ACCAAATTCC ACCAGTATGC AATGAATGGG 
GAAAAAGACA TCAACAACAA TGTGGAGAAA GCCCCCTGTG CCACCTCCAG TCCAGTGACA
CAGGATGACC TTCAGTATCA CAACCTCAGC AAGCAGCAGA ATGAGTCCCC GCAGCCCCTC
GTGGAGACGG GAAAGAAGTC TCCAGAATCT CTGGTCAAGC TGGATGCAAC CCCATTGTCC
TCCCCACGGC ATGTGAGGAT CAAAAACTGG GGCAGCGGGA TGACTTTCCA AGACACACTT
CACCATAAGG CCAAAGGGAT TTTAACTTGC AGGTCCAAAT CTTGCCTGGG GTCCATTATG
ACTCCCAAAA GTTTGACCAG AGGACCCAGG GACAAGCCTA CCCCTCCAGA TGAGCTTCTA
CCTCAAGCTA TCGAATTTGT CAACCAATAT TACGGCTCCT TCAAAGAGGC AAAAATAGAG
GAACATCTGG CCAGGGTGGA AGCGGTAACA AAGGAGATAG AAACAACAGG AACCTACCAA
CTGACGGGAG ATGAGCTCAT CTTCGCCACC AAGCAGGCCT GGCGCAATGC CCCACGCTGC
ATTGGGAGGA TCCAGTGGTC CAACCTGCAG GTCTTCGATG CCCGCAGCTG TTCCACTGCC
CGGGAAATGT TTGAACACAT CTGCAGACAC GTGCGTTACT CCACCAACAA TGGCAACATC
AGGTCGGCCA TCACCGTGTT CCCCCAGCGG AGTGATGGCA AGCACGACTT CCGGGTGTGG
AATGCTCAGC TCATCCGCTA TGCTGGCTAC CAGATGCCAG ATGGCAGCAT CAGAGGGGAC
CCTGCCAACG TGGAATTCAC TCAGCTGTGC ATCGACCTGG GCTGGAAGCC CAAGTACGGC
CGCTTCGATG TGGTCCCCCT GGTCCTGCAG GCCAATGGCC GTGACCCTGA GCTCTTCGAA
ATCCCACCTG ACCTTGTGCT TGAGGTGGCC ATGGAACATC CCAAATACGA GTGGTTTCGG
GAACTGGAGC TAAAGTGGTA CGCCCTGCCT GCAGTGGCCA ACATGCTGCT TGAGGTGGGC
GGCCTGGAGT TCCCAGGGTG CCCCTTCAAT GGCTGGTACA TGGGCACAGA GATCGGAGTC
CGGGACTTCT GTGACGTCCA GCGCTACAAC ATCCTGGAGG AAGTGGGCAG GAGAATGGGC
CTGGAAACGC ACAAGCTGGC CTCGCTCTGG AAAGACCAGG CTGTCGTTGA GATCAACATT
GCTGTGCTCC ATAGTTTCCA GAAGCAGAAT GTGACCATCA TGGACCACCA CTCGGCTGCA
GAATCCTTCA TGAAGTACAT GCAGAATGAA TACCGGTCCC GTGGGGGCTG CCCGGCAGAC
TGGATTTGGC TGGTCCCTCC CATGTCTGGG AGCATCACCC CCGTGTTTCA CCAGGAGATG
CTGAACTACG TCCTGTCCCC TTTCTACTAC TATCAGGTAG AGGCCTGGAA AACCCATGTC
TGGCAGGACG AGAAGCGGAG ACCCAAGAGA AGAGAGATTC CATTGAAAGT CTTGGTCAAA
GCTGTGCTCT TTGCCTGTAT GCTGATGCGC AAGACAATGG CGTCCCGAGT CAGAGTCACC
ATCCTCTTTG CGACAGAGAC AGGAAAATCA GAGGCGCTGG CCTGGGACCT GGGGGCCTTA
TTCAGCTGTG CCTTCAACCC CAAGGTTGTC TGCATGGATA AGTACAGGCT GAGCTGCCTG
GAGGAGGAAC GGCTGCTGTT GGTGGTGACC AGTACGTTTG GCAATGGAGA CTGCCCTGGC
AATGGAGAGA AACTGAAGAA ATCGCTCTTC ATGCTGAAAG AGCTCAACAA CAAATTCAGG
TACGCTGTGT TTGGCCTCGG CTCCAGCATG TACCCTCGGT TCTGCGCCTT TGCTCATGAC
ATTGATCAGA AGCTGTCCCA CCTGGGGGCC TCTCAGCTCA CCCCGATGGG AGAAGGGGAT
GAGCTCAGTG GGCAGGAGGA CGCCTTCCGC AGCTGGGCCG TGCAAACCTT CAAGGCAGCC
TGTGAGACGT TTGATGTCCG AGGCAAACAG CACATTCAGA TCCCCAAGCT CTACACCTCC
AATGTGACCT GGGACCCGCA CCACTACAGG CTCGTGCAGG ACTCACAGCC TTTGGACCTC
AGCAAAGCCC TCAGCAGCAT GCATGCCAAG AACGTGTTCA CCATGAGGCT CAAATCTCGG
CAGAATCTAC AAAGTCCGAC ATCCAGCCGT GCCACCATCC TGGTGGAACT CTCCTGTGAG
GATGGCCAAG GCCTGAACTA CCTGCCGGGG GAGCACCTTG GGGTTTGCCC AGGCAACCAG
CCGGCCCTGG TCCAAGGTAT CCTGGAGCGA GTGGTGGATG GCCCCACACC CCACCAGACA
GTGCGCCTGG AGGCCCTGGA TGAGAGTGGC AGCTACTGGG TCAGTGACAA GAGGCTGCCC
CCCTGCTCAC TCAGCCAGGC CCTCACCTAC TTCCTGGACA TCACCACACC CCCAACCCAG
CTGCTGCTCC AAAAGCTGGC CCAGGTGGCC ACAGAAGAGC CTGAGAGACA GAGGCTGGAG
GCCCTGTGCC AGCCCTCAGA GTACAGCAAG TGGAAGTTCA CCAACAGCCC CACATTCCTG
GAGGTGCTAG AGGAGTTCCC GTCCCTGCGG GTGTCTGCTG GCTTCCTGCT TTCCCAGCTC
CCCATTCTGA AGCCCAGGTT CTACTCCATC AGCTCCTCCC GGGATCACAC GCCCACAGAG
ATCCACCTGA CTGTGGCCGT GGTCACCTAC CACACCCGAG ATGGCCAGGG TCCCCTGCAC
CACGGCGTCT GCAGCACATG GCTCAACAGC CTGAAGCCCC AAGACCCAGT GCCCTGCTTT
GTGCGGAATG CCAGCGGCTT CCACCTCCCC GAGGATCCCT CCCATCCTTG CATCCTCATC
GGGCCTGGCA CAGGCATCGC GCCCTTCCGC AGTTTCTGGC AGCAACGGCT CCATGACTCC
CAGCACAAGG GAGTGCGGGG AGGCCGCATG ACCTTGGTGT TTGGGTGCCG CCGCCCAGAT
GAGGACCACA TCTACCAGGA GGAGATGCTG GAGATGGCCC AGAAGGGGGT GCTGCATGCG
GTGCACACAG CCTATTCCCG CCTGCCTGGC AAGCCCAAGG TCTATGTTCA GGACATCCTG
CGGCAGCAGC TGGCCAGCGA GGTGCTCCGT GTGCTCCACA AGGAGCCAGG CCACCTCTAT
GTTTGCGGGG ATGTGCGCAT GGCCCGGGAC GTGGCCCACA CCCTGAAGCA GCTGGTGGCT
GCCAAGCTGA AATTGAATGA GGAGCAGGTC GAGGACTATT TCTTTCAGCT CAAGAGCCAG
AAGCGCTATC ACGAAGATAT CTTTGGTGCT GTATTTCCTT ACGAGGCGAA GAAGGACAGG
GTGGCGGTGC AGCCCAGCAG CCTGGAGATG TCAGCGCTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000616.3
CDS265..3726
Misc Feature(1)211..213(+)
Misc Feature(2)514..1752(+)
Misc Feature(3)532..1713(+)
Misc Feature(4)862..1737(+)
Misc Feature(5)1159..1161(+)
Misc Feature(6)1270..1272(+)
Misc Feature(7)1600..1602(+)
Misc Feature(8)1789..1851(+)
Misc Feature(9)1876..3645(+)
Misc Feature(10)1885..2280(+)
Misc Feature(11)2356..2358(+)
Misc Feature(12)2470..3660(+)
Misc Feature(13)2578..3216(+)
Misc Feature(14)2980..2991(+)
Misc Feature(15)3088..3126(+)
Misc Feature(16)3199..3228(+)
Misc Feature(17)3214..3516(+)
Exon (1)1..191
Gene:NOS2
Gene Synonym:
Exon (2)192..374
Gene:NOS2
Gene Synonym:
Exon (3)375..459
Gene:NOS2
Gene Synonym:
Exon (4)460..582
Gene:NOS2
Gene Synonym:
Exon (5)583..731
Gene:NOS2
Gene Synonym:
Exon (6)732..894
Gene:NOS2
Gene Synonym:
Exon (7)895..986
Gene:NOS2
Gene Synonym:
Exon (8)987..1128
Gene:NOS2
Gene Synonym:
Exon (9)1129..1268
Gene:NOS2
Gene Synonym:
Exon (10)1269..1443
Gene:NOS2
Gene Synonym:
Exon (11)1444..1545
Gene:NOS2
Gene Synonym:
Exon (12)1546..1740
Gene:NOS2
Gene Synonym:
Exon (13)1741..1823
Gene:NOS2
Gene Synonym:
Exon (14)1824..1968
Gene:NOS2
Gene Synonym:
Exon (15)1969..2073
Gene:NOS2
Gene Synonym:
Exon (16)2074..2123
Gene:NOS2
Gene Synonym:
Exon (17)2124..2298
Gene:NOS2
Gene Synonym:
Exon (18)2299..2431
Gene:NOS2
Gene Synonym:
Exon (19)2432..2510
Gene:NOS2
Gene Synonym:
Exon (20)2511..2692
Gene:NOS2
Gene Synonym:
Exon (21)2693..2856
Gene:NOS2
Gene Synonym:
Exon (22)2857..3064
Gene:NOS2
Gene Synonym:
Exon (23)3065..3152
Gene:NOS2
Gene Synonym:
Exon (24)3153..3274
Gene:NOS2
Gene Synonym:
Exon (25)3275..3423
Gene:NOS2
Gene Synonym:
Exon (26)3424..3618
Gene:NOS2
Gene Synonym:
Exon (27)3619..4206
Gene:NOS2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000625
Organism Homo sapiens (human)
Definition Homo sapiens nitric oxide synthase 2, inducible (NOS2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000625

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGGCCTGTC CTTGGAAATT TCTGTTCAAG ACCAAATTCC ACCAGTATGC AATGAATGGG 
GAAAAAGACA TCAACAACAA TGTGGAGAAA GCCCCCTGTG CCACCTCCAG TCCAGTGACA
CAGGATGACC TTCAGTATCA CAACCTCAGC AAGCAGCAGA ATGAGTCCCC GCAGCCCCTC
GTGGAGACGG GAAAGAAGTC TCCAGAATCT CTGGTCAAGC TGGATGCAAC CCCATTGTCC
TCCCCACGGC ATGTGAGGAT CAAAAACTGG GGCAGCGGGA TGACTTTCCA AGACACACTT
CACCATAAGG CCAAAGGGAT TTTAACTTGC AGGTCCAAAT CTTGCCTGGG GTCCATTATG
ACTCCCAAAA GTTTGACCAG AGGACCCAGG GACAAGCCTA CCCCTCCAGA TGAGCTTCTA
CCTCAAGCTA TCGAATTTGT CAACCAATAT TACGGCTCCT TCAAAGAGGC AAAAATAGAG
GAACATCTGG CCAGGGTGGA AGCGGTAACA AAGGAGATAG AAACAACAGG AACCTACCAA
CTGACGGGAG ATGAGCTCAT CTTCGCCACC AAGCAGGCCT GGCGCAATGC CCCACGCTGC
ATTGGGAGGA TCCAGTGGTC CAACCTGCAG GTCTTCGATG CCCGCAGCTG TTCCACTGCC
CGGGAAATGT TTGAACACAT CTGCAGACAC GTGCGTTACT CCACCAACAA TGGCAACATC
AGGTCGGCCA TCACCGTGTT CCCCCAGCGG AGTGATGGCA AGCACGACTT CCGGGTGTGG
AATGCTCAGC TCATCCGCTA TGCTGGCTAC CAGATGCCAG ATGGCAGCAT CAGAGGGGAC
CCTGCCAACG TGGAATTCAC TCAGCTGTGC ATCGACCTGG GCTGGAAGCC CAAGTACGGC
CGCTTCGATG TGGTCCCCCT GGTCCTGCAG GCCAATGGCC GTGACCCTGA GCTCTTCGAA
ATCCCACCTG ACCTTGTGCT TGAGGTGGCC ATGGAACATC CCAAATACGA GTGGTTTCGG
GAACTGGAGC TAAAGTGGTA CGCCCTGCCT GCAGTGGCCA ACATGCTGCT TGAGGTGGGC
GGCCTGGAGT TCCCAGGGTG CCCCTTCAAT GGCTGGTACA TGGGCACAGA GATCGGAGTC
CGGGACTTCT GTGACGTCCA GCGCTACAAC ATCCTGGAGG AAGTGGGCAG GAGAATGGGC
CTGGAAACGC ACAAGCTGGC CTCGCTCTGG AAAGACCAGG CTGTCGTTGA GATCAACATT
GCTGTGCTCC ATAGTTTCCA GAAGCAGAAT GTGACCATCA TGGACCACCA CTCGGCTGCA
GAATCCTTCA TGAAGTACAT GCAGAATGAA TACCGGTCCC GTGGGGGCTG CCCGGCAGAC
TGGATTTGGC TGGTCCCTCC CATGTCTGGG AGCATCACCC CCGTGTTTCA CCAGGAGATG
CTGAACTACG TCCTGTCCCC TTTCTACTAC TATCAGGTAG AGGCCTGGAA AACCCATGTC
TGGCAGGACG AGAAGCGGAG ACCCAAGAGA AGAGAGATTC CATTGAAAGT CTTGGTCAAA
GCTGTGCTCT TTGCCTGTAT GCTGATGCGC AAGACAATGG CGTCCCGAGT CAGAGTCACC
ATCCTCTTTG CGACAGAGAC AGGAAAATCA GAGGCGCTGG CCTGGGACCT GGGGGCCTTA
TTCAGCTGTG CCTTCAACCC CAAGGTTGTC TGCATGGATA AGTACAGGCT GAGCTGCCTG
GAGGAGGAAC GGCTGCTGTT GGTGGTGACC AGTACGTTTG GCAATGGAGA CTGCCCTGGC
AATGGAGAGA AACTGAAGAA ATCGCTCTTC ATGCTGAAAG AGCTCAACAA CAAATTCAGG
TACGCTGTGT TTGGCCTCGG CTCCAGCATG TACCCTCGGT TCTGCGCCTT TGCTCATGAC
ATTGATCAGA AGCTGTCCCA CCTGGGGGCC TCTCAGCTCA CCCCGATGGG AGAAGGGGAT
GAGCTCAGTG GGCAGGAGGA CGCCTTCCGC AGCTGGGCCG TGCAAACCTT CAAGGCAGCC
TGTGAGACGT TTGATGTCCG AGGCAAACAG CACATTCAGA TCCCCAAGCT CTACACCTCC
AATGTGACCT GGGACCCGCA CCACTACAGG CTCGTGCAGG ACTCACAGCC TTTGGACCTC
AGCAAAGCCC TCAGCAGCAT GCATGCCAAG AACGTGTTCA CCATGAGGCT CAAATCTCGG
CAGAATCTAC AAAGTCCGAC ATCCAGCCGT GCCACCATCC TGGTGGAACT CTCCTGTGAG
GATGGCCAAG GCCTGAACTA CCTGCCGGGG GAGCACCTTG GGGTTTGCCC AGGCAACCAG
CCGGCCCTGG TCCAAGGTAT CCTGGAGCGA GTGGTGGATG GCCCCACACC CCACCAGACA
GTGCGCCTGG AGGCCCTGGA TGAGAGTGGC AGCTACTGGG TCAGTGACAA GAGGCTGCCC
CCCTGCTCAC TCAGCCAGGC CCTCACCTAC TTCCTGGACA TCACCACACC CCCAACCCAG
CTGCTGCTCC AAAAGCTGGC CCAGGTGGCC ACAGAAGAGC CTGAGAGACA GAGGCTGGAG
GCCCTGTGCC AGCCCTCAGA GTACAGCAAG TGGAAGTTCA CCAACAGCCC CACATTCCTG
GAGGTGCTAG AGGAGTTCCC GTCCCTGCGG GTGTCTGCTG GCTTCCTGCT TTCCCAGCTC
CCCATTCTGA AGCCCAGGTT CTACTCCATC AGCTCCTCCC GGGATCACAC GCCCACAGAG
ATCCACCTGA CTGTGGCCGT GGTCACCTAC CACACCCGAG ATGGCCAGGG TCCCCTGCAC
CACGGCGTCT GCAGCACATG GCTCAACAGC CTGAAGCCCC AAGACCCAGT GCCCTGCTTT
GTGCGGAATG CCAGCGGCTT CCACCTCCCC GAGGATCCCT CCCATCCTTG CATCCTCATC
GGGCCTGGCA CAGGCATCGC GCCCTTCCGC AGTTTCTGGC AGCAACGGCT CCATGACTCC
CAGCACAAGG GAGTGCGGGG AGGCCGCATG ACCTTGGTGT TTGGGTGCCG CCGCCCAGAT
GAGGACCACA TCTACCAGGA GGAGATGCTG GAGATGGCCC AGAAGGGGGT GCTGCATGCG
GTGCACACAG CCTATTCCCG CCTGCCTGGC AAGCCCAAGG TCTATGTTCA GGACATCCTG
CGGCAGCAGC TGGCCAGCGA GGTGCTCCGT GTGCTCCACA AGGAGCCAGG CCACCTCTAT
GTTTGCGGGG ATGTGCGCAT GGCCCGGGAC GTGGCCCACA CCCTGAAGCA GCTGGTGGCT
GCCAAGCTGA AATTGAATGA GGAGCAGGTC GAGGACTATT TCTTTCAGCT CAAGAGCCAG
AAGCGCTATC ACGAAGATAT CTTTGGTGCT GTATTTCCTT ACGAGGCGAA GAAGGACAGG
GTGGCGGTGC AGCCCAGCAG CCTGGAGATG TCAGCGCTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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