DBR1 cDNA ORF clone, Homo sapiens(human)
| Gene Symbol | DBR1 |
| Entrez Gene ID | 51163 |
| Full Name | debranching RNA lariats 1 |
| General protein information |
|
| Gene Type | protein-coding |
| Organism | Homo sapiens(human) |
| Genome | |
| Summary | The protein encoded by this gene is an RNA lariat debranching enzyme that hydrolyzes 2'-5' prime branched phosphodiester bonds. The encoded protein specifically targets the bonds at the branch point of excised lariat intron RNA, converting them to linear molecules that are then degraded. This protein may also be involved in retroviral replication. [provided by RefSeq, Nov 2011]. |
| Disorder MIM: |
mRNA and Protein(s)
| mRNA | Protein | Name |
|---|---|---|
| NM_016216.4 | NP_057300.2 | lariat debranching enzyme |
| NM_016216.3 | NP_057300.2 | lariat debranching enzyme |
| Danio rerio (zebrafish) | dbr1 | NP_955947.1 |
| Caenorhabditis elegans (roundworm) | dbr-1 | NP_491868.2 |
| Schizosaccharomyces pombe (fission yeast) | dbr1 | NP_593470.2 |
| Magnaporthe oryzae (rice blast fungus) | MGG_03897 | XP_003719979.1 |
| Neurospora crassa | NCU03409 | XP_956856.1 |
| Bos taurus (cattle) | DBR1 | NP_001073759.1 |
| Homo sapiens (human) | DBR1 | NP_057300.2 |
| DBR1 | XP_001114637.1 | |
| Rattus norvegicus (Norway rat) | Dbr1 | NP_001102907.1 |
| Gallus gallus (chicken) | DBR1 | NP_001026737.1 |
| Pan troglodytes (chimpanzee) | DBR1 | XP_526319.2 |
| Drosophila melanogaster (fruit fly) | ldbr | NP_996022.1 |
| Oryza sativa (rice) | Os03g0725300 | NP_001051129.2 |
| Xenopus tropicalis (tropical clawed frog) | dbr1 | NP_988947.1 |
| Canis lupus familiaris (dog) | DBR1 | XP_852077.1 |
| Mus musculus (house mouse) | Dbr1 | NP_113580.2 |
| Anopheles gambiae (African malaria mosquito) | AgaP_AGAP006805 | XP_308942.4 |
| Arabidopsis thaliana (thale cress) | DBR1 | NP_567881.1 |
Related articles in PubMed
Conformational Changes in the 5' End of the HIV-1 Genome Dependent on the Debranching Enzyme DBR1 during Early Stages of Infection.
Galvis AE, Fisher HE, Fan H, Camerini D
Journal of virology91(23)(2017 Dec)
Human DBR1 modulates the recycling of snRNPs to affect alternative RNA splicing and contributes to the suppression of cancer development.
Han B, Park HK, Ching T, Panneerselvam J, Wang H, Shen Y, Zhang J, Li L, Che R, Garmire L, Fei P
Oncogene36(38)5382-5391(2017 Sep)
In silico functional dissection of saturation mutagenesis: Interpreting the relationship between phenotypes and changes in protein stability, interactions and activity.
Pires DE, Chen J, Blundell TL, Ascher DB
Scientific reports619848(2016 Jan)
Identification of the specific interactors of the human lariat RNA debranching enzyme 1 protein.
Masaki S, Yoshimoto R, Kaida D, Hata A, Satoh T, Ohno M, Kataoka N
International journal of molecular sciences16(2)3705-21(2015 Feb)
Impairment of HIV-1 cDNA synthesis by DBR1 knockdown.
Galvis AE, Fisher HE, Nitta T, Fan H, Camerini D
Journal of virology88(12)7054-69(2014 Jun)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
findings support a new view of the early steps in HIV genome replication - they support a model in which lariat formation of viral genomic RNA is important for minus-strand transfer, with DBR1 being required to resolve the lariat to allow reverse transcription to proceed
Title: Conformational Changes in the 5' End of the HIV-1 Genome Dependent on the Debranching Enzyme DBR1 during Early Stages of Infection.
Transcriptomic sequencing confirmed the effect of hDBR1 on RNA splicing, and metabolite profiling supported the observation that neoplasm is triggered by a decrease in hDBR1 expression both in vitro and in vivo.
Title: Human DBR1 modulates the recycling of snRNPs to affect alternative RNA splicing and contributes to the suppression of cancer development.
saturation mutagenesis of DBR1 and Gal4 and show that the experimental phenotypes for over 80% of the mutations correlate well with predicted effects of mutations on protein stability and RNA binding affinity.
Title: In silico functional dissection of saturation mutagenesis: Interpreting the relationship between phenotypes and changes in protein stability, interactions and activity.
Results of protein-protein interaction between human Dbr1 and factors found in the Intron Large complex identify Xab2 and a novel protein CWF19L1 as specific interactors of DBR1.
Title: Identification of the specific interactors of the human lariat RNA debranching enzyme 1 protein.
Inhibition of DBR1 caused a significant reduction in the formation of intermediate and full-length HIV-1 cDNA.
Title: Impairment of HIV-1 cDNA synthesis by DBR1 knockdown.
The following DBR1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DBR1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OHu11162 |
|
| Clone ID Related Accession (Same CDS sequence) | NM_016216.4 , NM_016216.3 | |
| Accession Version | NM_016216.4 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1635bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2020-01-01 | |
| Organism | Homo sapiens(human) | |
| Product | lariat debranching enzyme | |
| Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL702928.1, BC009472.1, AC023049.14, CD677175.1 and BF987317.1. On May 17, 2019 this sequence version replaced NM_016216.3. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.257390.1, SRR7346977.766999.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2150385 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000260803.9/ ENSP00000260803.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGCGGGTGG CTGTGGCTGG CTGCTGCCAC GGCGAGCTGG ATAAGATCTA TGAGACGCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_057300.2 |
| CDS | 127..1761 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_016216.4 |
ORF Insert Sequence:
1 | ATGCGGGTGG CTGTGGCTGG CTGCTGCCAC GGCGAGCTGG ATAAGATCTA TGAGACGCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu11162 |
|
| Clone ID Related Accession (Same CDS sequence) | NM_016216.4 , NM_016216.3 | |
| Accession Version | NM_016216.3 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1635bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-02-21 | |
| Organism | Homo sapiens(human) | |
| Product | lariat debranching enzyme | |
| Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL702928.1, BC009472.1, AC023049.14, CD677175.1 and BF987317.1. On Aug 3, 2010 this sequence version replaced NM_016216.2. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.198817.1, SRR1803614.188181.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2150385 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGCGGGTGG CTGTGGCTGG CTGCTGCCAC GGCGAGCTGG ATAAGATCTA TGAGACGCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_057300.2 |
| CDS | 155..1789 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_016216.3 |
ORF Insert Sequence:
1 | ATGCGGGTGG CTGTGGCTGG CTGCTGCCAC GGCGAGCTGG ATAAGATCTA TGAGACGCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
 |
Conformational Changes in the 5' End of the HIV-1 Genome Dependent on the Debranching Enzyme DBR1 during Early Stages of Infection. |
|
Human DBR1 modulates the recycling of snRNPs to affect alternative RNA splicing and contributes to the suppression of cancer development. |
|
In silico functional dissection of saturation mutagenesis: Interpreting the relationship between phenotypes and changes in protein stability, interactions and activity. |
|
Identification of the specific interactors of the human lariat RNA debranching enzyme 1 protein. |
|
Impairment of HIV-1 cDNA synthesis by DBR1 knockdown. |