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PDHB cDNA ORF clone, Homo sapiens(Human)

Gene Symbol PDHB
Entrez Gene ID 5162
Full Name pyruvate dehydrogenase E1 beta subunit
Synonyms PDHBD,PDHE1-B,PDHE1B,PHE1B
General protein information
Preferred Names

pyruvate dehydrogenase E1 beta subunit

Names

pyruvate dehydrogenase E1 component subunit beta, mitochondrial
pyruvate dehydrogenase (lipoamide) beta
pyruvate dehydrogenase, E1 beta polypeptide

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

3

3p14.3

Summary The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and carbon dioxide, and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 beta subunit. Mutations in this gene are associated with pyruvate dehydrogenase E1-beta deficiency. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2012].
Disorder MIM:

179060

Disorder Html: Pyruvate dehydrogenase E1-beta deficiency (3)

mRNA and Protein(s)

mRNA Protein Name
NM_001173468.1 NP_001166939.1 pyruvate dehydrogenase E1 component subunit beta, mitochondrial isoform 2 precursor
NM_001315536.1 NP_001302465.1 pyruvate dehydrogenase E1 component subunit beta, mitochondrial isoform 3
NM_000925.3 NP_000916.2 pyruvate dehydrogenase E1 component subunit beta, mitochondrial isoform 1 precursor



Schizosaccharomyces pombe (fission yeast) pdb1 NP_596272.1
Bos taurus (cattle) PDHB NP_001030512.2
Danio rerio (zebrafish) pdhb NP_998319.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP010421 XP_311527.2
Neurospora crassa NCU03004 XP_965390.1
Xenopus tropicalis (tropical clawed frog) pdhb NP_001016212.1
Mus musculus (house mouse) Pdhb NP_077183.1
Rattus norvegicus (Norway rat) Pdhb NP_001007621.1
Gallus gallus (chicken) PDHB NP_001185549.1
Caenorhabditis elegans (roundworm) pdhb-1 NP_500340.1
Saccharomyces cerevisiae (baker's yeast) PDB1 NP_009780.1
Kluyveromyces lactis KLLA0F09603g XP_455516.1
Arabidopsis thaliana (thale cress) MAB1 NP_199898.1
Oryza sativa (rice) Os08g0536000 NP_001062360.1
Homo sapiens (human) PDHB NP_000916.2
Pan troglodytes (chimpanzee) PDHB NP_001233347.1
Canis lupus familiaris (dog) PDHB XP_533778.2
Magnaporthe oryzae (rice blast fungus) MGG_10569 XP_003721383.1
Macaca mulatta (rhesus monkey) PDHB XP_001096445.1
Drosophila melanogaster (fruit fly) CG11876 NP_651668.1
Eremothecium gossypii AGOS_AAR167C NP_982710.1
Oryza sativa (rice) Os09g0509200 NP_001063627.1


Related articles in PubMed

Pyruvate dehydrogenase B promoted the growth and migration of the nasopharyngeal carcinoma cells.
Tang H, Luo X, Li J, Zhou Y, Li Y, Song L, Zhang X, Chen T
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine37(8)10563-9(2016 Aug)

ATP6AP2/(pro)renin receptor contributes to glucose metabolism via stabilizing the pyruvate dehydrogenase E1 β subunit.
Kanda A, Noda K, Ishida S
The Journal of biological chemistry290(15)9690-700(2015 Apr)

Prolyl-hydroxylase PHD3 interacts with pyruvate dehydrogenase (PDH)-E1β and regulates the cellular PDH activity.
Kikuchi D, Minamishima YA, Nakayama K
Biochemical and biophysical research communications451(2)288-94(2014 Aug)

Proteomic characterization of the human sperm nucleus.
de Mateo S, Castillo J, Estanyol JM, Ballescà JL, Oliva R
Proteomics11(13)2714-26(2011 Jul)

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
Hendrickson SL, Lautenberger JA, Chinn LW, Malasky M, Sezgin E, Kingsley LA, Goedert JJ, Kirk GD, Gomperts ED, Buchbinder SP, Troyer JL, O'Brien SJ
PloS one5(9)e12862(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PDHB was downregulated in nasopharyngeal carcinoma cells. Forced expression of PDHB in NPC cells inhibited cell growth and migration, while knocking down the expression of PDHB promoted the growth, migration, and tumorigenesis of NPC cells. PDHB inhibited ERK signaling and cell growth driven by RasV12.
Title: Pyruvate dehydrogenase B promoted the growth and migration of the nasopharyngeal carcinoma cells.

Data indicate that (pro)renin receptor ATP6PA2 interacts with the E1 beta subunit of pyruvate dehydrogenase and controlling its protein stability
Title: ATP6AP2/(pro)renin receptor contributes to glucose metabolism via stabilizing the pyruvate dehydrogenase E1 β subunit.

PHD3 depletion did not affect the expression of the PDH-E1alpha, E1beta, and E2 subunits, or the phosphorylation status of E1alpha, but destabilized the PDH complex (PDC), resulting in less functional PDC.
Title: Prolyl-hydroxylase PHD3 interacts with pyruvate dehydrogenase (PDH)-E1β and regulates the cellular PDH activity.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

Sequencing of PDHB reveals a homozygous point mutation (c.302T>C), causing a predicted amino acid change in patient 1. Patient 2 is compound heterozygote for mutations c.301A>G (p.M101V) and c.313G>A (p.R105Q).
Title: PDH E1β deficiency with novel mutations in two patients with Leigh syndrome.

The following PDHB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDHB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu18263
NM_000925.3
Latest version!
Homo sapiens pyruvate dehydrogenase (lipoamide) beta (PDHB), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu09402 NM_001173468.1
Latest version!
Homo sapiens pyruvate dehydrogenase (lipoamide) beta (PDHB), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $202.30
$289.00
OHu102065 NM_001315536.1
Latest version!
Homo sapiens pyruvate dehydrogenase (lipoamide) beta (PDHB), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $223.30
$319.00

ORF Online Only Promotion

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2-4 Clone 40% OFF
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All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu18263
Clone ID Related Accession (Same CDS sequence) NM_000925.3
Accession Version NM_000925.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1080bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-08
Organism Homo sapiens(Human)
Product pyruvate dehydrogenase E1 component subunit beta, mitochondrial isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA965753.1, AK225569.1, AK293153.1 and BM971427.1. This sequence is a reference standard in the RefSeqGene project. On Mar 16, 2010 this sequence version replaced NM_000925.2. Transcript Variant: This variant (1) represents the predominant transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK225621.1, SRR1163657.552919.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2146411 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCGGCGG TGTCTGGCTT GGTGCGGAGA CCCCTTCGGG AGGTCTCCGG GCTGCTGAAG 
AGGCGCTTTC ACTGGACCGC GCCGGCTGCG CTGCAGGTGA CAGTTCGTGA TGCTATAAAT
CAGGGTATGG ATGAGGAGCT GGAAAGAGAT GAGAAGGTAT TTCTGCTTGG AGAAGAAGTT
GCCCAGTATG ATGGGGCATA CAAGGTTAGT CGAGGGCTGT GGAAGAAATA TGGAGACAAG
AGGATTATTG ACACTCCCAT ATCAGAGATG GGCTTTGCTG GAATTGCTGT AGGTGCAGCT
ATGGCTGGGT TGCGGCCCAT TTGTGAATTT ATGACCTTCA ATTTCTCCAT GCAAGCCATT
GACCAGGTTA TAAACTCAGC TGCCAAGACC TACTACATGT CTGGTGGCCT TCAGCCTGTG
CCTATAGTCT TCAGGGGGCC CAATGGTGCC TCAGCAGGTG TAGCTGCCCA GCACTCACAG
TGCTTTGCTG CCTGGTATGG GCACTGCCCA GGCTTAAAGG TGGTCAGTCC CTGGAATTCA
GAGGATGCTA AAGGACTTAT TAAATCAGCC ATTCGGGATA ACAATCCAGT GGTGGTGCTA
GAGAATGAAT TGATGTATGG GGTTCCTTTT GAATTTCCTC CGGAAGCTCA GTCAAAAGAT
TTTCTGATTC CTATTGGAAA AGCCAAAATA GAAAGGCAAG GAACACATAT AACTGTGGTT
TCCCATTCAA GACCTGTGGG CCACTGCTTA GAAGCTGCAG CAGTGCTATC TAAAGAAGGA
GTTGAATGTG AGGTGATAAA TATGCGTACC ATTAGACCAA TGGACATGGA AACCATAGAA
GCCAGTGTCA TGAAGACAAA TCATCTTGTA ACTGTGGAAG GAGGCTGGCC ACAGTTTGGA
GTAGGAGCTG AAATCTGTGC CAGGATCATG GAAGGTCCTG CGTTCAATTT CCTGGATGCT
CCTGCTGTTC GTGTCACTGG TGCTGATGTC CCTATGCCTT ATGCAAAGAT TCTAGAGGAC
AACTCTATAC CTCAGGTCAA AGACATCATA TTTGCAATAA AGAAAACATT AAATATTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000916.2
CDS44..1123
Misc Feature(1)59..1111
Misc Feature(2)152..652
Misc Feature(3)order(194..196,200..202,218..220,284..286,293..295,299..316,323..328,332..340,395..397,404..409,434..439,503..505,512..514,557..562,623..628)
Misc Feature(4)order(215..217,302..304,308..310,386..388,395..397)
Misc Feature(5)order(218..220,293..295,299..310,392..397,401..403,416..418,428..430,437..439,503..505,509..514,536..538,542..547,551..553)
Misc Feature(6)242..244
Misc Feature(7)719..1090
Misc Feature(8)998..1000
Misc Feature(9)1103..1105
Exon (1)1..85
Exon (2)86..139
Exon (3)140..247
Exon (4)248..310
Exon (5)311..346
Exon (6)347..632
Exon (7)633..743
Exon (8)744..835
Exon (9)836..977
Exon (10)978..1527
Translation

Target ORF information:

RefSeq Version NM_000925.3
Organism Homo sapiens(Human)
Definition Homo sapiens pyruvate dehydrogenase (lipoamide) beta (PDHB), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000925.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCGGCGG TGTCTGGCTT GGTGCGGAGA CCCCTTCGGG AGGTCTCCGG GCTGCTGAAG 
AGGCGCTTTC ACTGGACCGC GCCGGCTGCG CTGCAGGTGA CAGTTCGTGA TGCTATAAAT
CAGGGTATGG ATGAGGAGCT GGAAAGAGAT GAGAAGGTAT TTCTGCTTGG AGAAGAAGTT
GCCCAGTATG ATGGGGCATA CAAGGTTAGT CGAGGGCTGT GGAAGAAATA TGGAGACAAG
AGGATTATTG ACACTCCCAT ATCAGAGATG GGCTTTGCTG GAATTGCTGT AGGTGCAGCT
ATGGCTGGGT TGCGGCCCAT TTGTGAATTT ATGACCTTCA ATTTCTCCAT GCAAGCCATT
GACCAGGTTA TAAACTCAGC TGCCAAGACC TACTACATGT CTGGTGGCCT TCAGCCTGTG
CCTATAGTCT TCAGGGGGCC CAATGGTGCC TCAGCAGGTG TAGCTGCCCA GCACTCACAG
TGCTTTGCTG CCTGGTATGG GCACTGCCCA GGCTTAAAGG TGGTCAGTCC CTGGAATTCA
GAGGATGCTA AAGGACTTAT TAAATCAGCC ATTCGGGATA ACAATCCAGT GGTGGTGCTA
GAGAATGAAT TGATGTATGG GGTTCCTTTT GAATTTCCTC CGGAAGCTCA GTCAAAAGAT
TTTCTGATTC CTATTGGAAA AGCCAAAATA GAAAGGCAAG GAACACATAT AACTGTGGTT
TCCCATTCAA GACCTGTGGG CCACTGCTTA GAAGCTGCAG CAGTGCTATC TAAAGAAGGA
GTTGAATGTG AGGTGATAAA TATGCGTACC ATTAGACCAA TGGACATGGA AACCATAGAA
GCCAGTGTCA TGAAGACAAA TCATCTTGTA ACTGTGGAAG GAGGCTGGCC ACAGTTTGGA
GTAGGAGCTG AAATCTGTGC CAGGATCATG GAAGGTCCTG CGTTCAATTT CCTGGATGCT
CCTGCTGTTC GTGTCACTGG TGCTGATGTC CCTATGCCTT ATGCAAAGAT TCTAGAGGAC
AACTCTATAC CTCAGGTCAA AGACATCATA TTTGCAATAA AGAAAACATT AAATATTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu09402
Clone ID Related Accession (Same CDS sequence) NM_001173468.1
Accession Version NM_001173468.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1026bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-08
Organism Homo sapiens(Human)
Product pyruvate dehydrogenase E1 component subunit beta, mitochondrial isoform 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA965753.1, AK293153.1 and BM971427.1. Transcript Variant: This variant (2) lacks a segment in the coding region compared to variant 1. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK293153.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1970526, SAMEA2146411 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCGGCGG TGTCTGGCTT GGTGCGGAGA CCCCTTCGGG AGGTCTCCGG GCTGCTGAAG 
AGGCGCTTTC ACTGGACCGC GCCGGCTGCG CTGCAGGTGA CAGTTCGTGA TGCTATAAAT
CAGGGTATGG ATGAGGAGCT GGAAAGAGAT GAGAAGGTAT TTCTGCTTGG AGAAGAAGTT
GCCCAGTATG ATGGGGCATA CAAGGTTAGT CGAGGGCTGT GGAAGAAATA TGGAGACAAG
AGGATTATTG ACACTCCCAT ATCAGAGATG GGCTTTGCTG GAATTGCTGT AGGTGCAGCT
ATGGCTGGGT TGCGGCCCAT TTGTGAATTT ATGACCTTCA ATTTCTCCAT GCAAGCCATT
GACCAGGTTA TAAACTCAGC TGCCAAGACC TACTACATGT CTGGTGTAGC TGCCCAGCAC
TCACAGTGCT TTGCTGCCTG GTATGGGCAC TGCCCAGGCT TAAAGGTGGT CAGTCCCTGG
AATTCAGAGG ATGCTAAAGG ACTTATTAAA TCAGCCATTC GGGATAACAA TCCAGTGGTG
GTGCTAGAGA ATGAATTGAT GTATGGGGTT CCTTTTGAAT TTCCTCCGGA AGCTCAGTCA
AAAGATTTTC TGATTCCTAT TGGAAAAGCC AAAATAGAAA GGCAAGGAAC ACATATAACT
GTGGTTTCCC ATTCAAGACC TGTGGGCCAC TGCTTAGAAG CTGCAGCAGT GCTATCTAAA
GAAGGAGTTG AATGTGAGGT GATAAATATG CGTACCATTA GACCAATGGA CATGGAAACC
ATAGAAGCCA GTGTCATGAA GACAAATCAT CTTGTAACTG TGGAAGGAGG CTGGCCACAG
TTTGGAGTAG GAGCTGAAAT CTGTGCCAGG ATCATGGAAG GTCCTGCGTT CAATTTCCTG
GATGCTCCTG CTGTTCGTGT CACTGGTGCT GATGTCCCTA TGCCTTATGC AAAGATTCTA
GAGGACAACT CTATACCTCA GGTCAAAGAC ATCATATTTG CAATAAAGAA AACATTAAAT
ATTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001166939.1
CDS44..1069
Misc Feature(1)59..1057
Misc Feature(2)152..598
Misc Feature(3)order(194..196,200..202,218..220,284..286,293..295,299..316,323..328,332..340,395..397,404..409,434..439,449..451,458..460,503..508,569..574)
Misc Feature(4)order(215..217,302..304,308..310,386..388,395..397)
Misc Feature(5)order(218..220,293..295,299..310,392..397,401..403,416..418,428..430,437..439,449..451,455..460,482..484,488..493,497..499)
Misc Feature(6)665..1036
Exon (1)1..85
Exon (2)86..139
Exon (3)140..247
Exon (4)248..310
Exon (5)311..346
Exon (6)347..446
Exon (7)447..578
Exon (8)579..689
Exon (9)690..781
Exon (10)782..923
Exon (11)924..1473
Translation

Target ORF information:

RefSeq Version NM_001173468.1
Organism Homo sapiens(Human)
Definition Homo sapiens pyruvate dehydrogenase (lipoamide) beta (PDHB), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001173468.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCGGCGG TGTCTGGCTT GGTGCGGAGA CCCCTTCGGG AGGTCTCCGG GCTGCTGAAG 
AGGCGCTTTC ACTGGACCGC GCCGGCTGCG CTGCAGGTGA CAGTTCGTGA TGCTATAAAT
CAGGGTATGG ATGAGGAGCT GGAAAGAGAT GAGAAGGTAT TTCTGCTTGG AGAAGAAGTT
GCCCAGTATG ATGGGGCATA CAAGGTTAGT CGAGGGCTGT GGAAGAAATA TGGAGACAAG
AGGATTATTG ACACTCCCAT ATCAGAGATG GGCTTTGCTG GAATTGCTGT AGGTGCAGCT
ATGGCTGGGT TGCGGCCCAT TTGTGAATTT ATGACCTTCA ATTTCTCCAT GCAAGCCATT
GACCAGGTTA TAAACTCAGC TGCCAAGACC TACTACATGT CTGGTGTAGC TGCCCAGCAC
TCACAGTGCT TTGCTGCCTG GTATGGGCAC TGCCCAGGCT TAAAGGTGGT CAGTCCCTGG
AATTCAGAGG ATGCTAAAGG ACTTATTAAA TCAGCCATTC GGGATAACAA TCCAGTGGTG
GTGCTAGAGA ATGAATTGAT GTATGGGGTT CCTTTTGAAT TTCCTCCGGA AGCTCAGTCA
AAAGATTTTC TGATTCCTAT TGGAAAAGCC AAAATAGAAA GGCAAGGAAC ACATATAACT
GTGGTTTCCC ATTCAAGACC TGTGGGCCAC TGCTTAGAAG CTGCAGCAGT GCTATCTAAA
GAAGGAGTTG AATGTGAGGT GATAAATATG CGTACCATTA GACCAATGGA CATGGAAACC
ATAGAAGCCA GTGTCATGAA GACAAATCAT CTTGTAACTG TGGAAGGAGG CTGGCCACAG
TTTGGAGTAG GAGCTGAAAT CTGTGCCAGG ATCATGGAAG GTCCTGCGTT CAATTTCCTG
GATGCTCCTG CTGTTCGTGT CACTGGTGCT GATGTCCCTA TGCCTTATGC AAAGATTCTA
GAGGACAACT CTATACCTCA GGTCAAAGAC ATCATATTTG CAATAAAGAA AACATTAAAT
ATTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu102065
Clone ID Related Accession (Same CDS sequence) NM_001315536.1
Accession Version NM_001315536.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1026bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product pyruvate dehydrogenase E1 component subunit beta, mitochondrial isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA965753.1, AL117618.1 and BQ477366.1. On Oct 9, 2015 this sequence version replaced XM_011533828.1. Transcript Variant: This variant (4) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (3) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL117618.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2146411 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCGGCGG TGTCTGGCTT GGTGCGGAGA CCCCTTCGGG AGGTGACAGT TCGTGATGCT 
ATAAATCAGG GTATGGATGA GGAGCTGGAA AGAGATGAGA AGGTATTTCT GCTTGGAGAA
GAAGTTGCCC AGTATGATGG GGCATACAAG GTTAGTCGAG GGCTGTGGAA GAAATATGGA
GACAAGAGGA TTATTGACAC TCCCATATCA GAGATGGGCT TTGCTGGAAT TGCTGTAGGT
GCAGCTATGG CTGGGTTGCG GCCCATTTGT GAATTTATGA CCTTCAATTT CTCCATGCAA
GCCATTGACC AGGTTATAAA CTCAGCTGCC AAGACCTACT ACATGTCTGG TGGCCTTCAG
CCTGTGCCTA TAGTCTTCAG GGGGCCCAAT GGTGCCTCAG CAGGTGTAGC TGCCCAGCAC
TCACAGTGCT TTGCTGCCTG GTATGGGCAC TGCCCAGGCT TAAAGGTGGT CAGTCCCTGG
AATTCAGAGG ATGCTAAAGG ACTTATTAAA TCAGCCATTC GGGATAACAA TCCAGTGGTG
GTGCTAGAGA ATGAATTGAT GTATGGGGTT CCTTTTGAAT TTCCTCCGGA AGCTCAGTCA
AAAGATTTTC TGATTCCTAT TGGAAAAGCC AAAATAGAAA GGCAAGGAAC ACATATAACT
GTGGTTTCCC ATTCAAGACC TGTGGGCCAC TGCTTAGAAG CTGCAGCAGT GCTATCTAAA
GAAGGAGTTG AATGTGAGGT GATAAATATG CGTACCATTA GACCAATGGA CATGGAAACC
ATAGAAGCCA GTGTCATGAA GACAAATCAT CTTGTAACTG TGGAAGGAGG CTGGCCACAG
TTTGGAGTAG GAGCTGAAAT CTGTGCCAGG ATCATGGAAG GTCCTGCGTT CAATTTCCTG
GATGCTCCTG CTGTTCGTGT CACTGGTGCT GATGTCCCTA TGCCTTATGC AAAGATTCTA
GAGGACAACT CTATACCTCA GGTCAAAGAC ATCATATTTG CAATAAAGAA AACATTAAAT
ATTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001302465.1
CDS44..1069
Misc Feature(1)80..1057
Misc Feature(2)98..598
Misc Feature(3)order(140..142,146..148,164..166,230..232,239..241,245..262,269..274,278..286,341..343,350..355,380..385,449..451,458..460,503..508,569..574)
Misc Feature(4)order(161..163,248..250,254..256,332..334,341..343)
Misc Feature(5)order(164..166,239..241,245..256,338..343,347..349,362..364,374..376,383..385,449..451,455..460,482..484,488..493,497..499)
Misc Feature(6)665..1036
Exon (1)1..85
Exon (2)86..193
Exon (3)194..256
Exon (4)257..292
Exon (5)293..578
Exon (6)579..689
Exon (7)690..781
Exon (8)782..923
Exon (9)924..1473
Translation

Target ORF information:

RefSeq Version NM_001315536.1
Organism Homo sapiens(Human)
Definition Homo sapiens pyruvate dehydrogenase (lipoamide) beta (PDHB), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001315536.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCGGCGG TGTCTGGCTT GGTGCGGAGA CCCCTTCGGG AGGTGACAGT TCGTGATGCT 
ATAAATCAGG GTATGGATGA GGAGCTGGAA AGAGATGAGA AGGTATTTCT GCTTGGAGAA
GAAGTTGCCC AGTATGATGG GGCATACAAG GTTAGTCGAG GGCTGTGGAA GAAATATGGA
GACAAGAGGA TTATTGACAC TCCCATATCA GAGATGGGCT TTGCTGGAAT TGCTGTAGGT
GCAGCTATGG CTGGGTTGCG GCCCATTTGT GAATTTATGA CCTTCAATTT CTCCATGCAA
GCCATTGACC AGGTTATAAA CTCAGCTGCC AAGACCTACT ACATGTCTGG TGGCCTTCAG
CCTGTGCCTA TAGTCTTCAG GGGGCCCAAT GGTGCCTCAG CAGGTGTAGC TGCCCAGCAC
TCACAGTGCT TTGCTGCCTG GTATGGGCAC TGCCCAGGCT TAAAGGTGGT CAGTCCCTGG
AATTCAGAGG ATGCTAAAGG ACTTATTAAA TCAGCCATTC GGGATAACAA TCCAGTGGTG
GTGCTAGAGA ATGAATTGAT GTATGGGGTT CCTTTTGAAT TTCCTCCGGA AGCTCAGTCA
AAAGATTTTC TGATTCCTAT TGGAAAAGCC AAAATAGAAA GGCAAGGAAC ACATATAACT
GTGGTTTCCC ATTCAAGACC TGTGGGCCAC TGCTTAGAAG CTGCAGCAGT GCTATCTAAA
GAAGGAGTTG AATGTGAGGT GATAAATATG CGTACCATTA GACCAATGGA CATGGAAACC
ATAGAAGCCA GTGTCATGAA GACAAATCAT CTTGTAACTG TGGAAGGAGG CTGGCCACAG
TTTGGAGTAG GAGCTGAAAT CTGTGCCAGG ATCATGGAAG GTCCTGCGTT CAATTTCCTG
GATGCTCCTG CTGTTCGTGT CACTGGTGCT GATGTCCCTA TGCCTTATGC AAAGATTCTA
GAGGACAACT CTATACCTCA GGTCAAAGAC ATCATATTTG CAATAAAGAA AACATTAAAT
ATTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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