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PDHB cDNA ORF clone, Homo sapiens (human)

Gene Symbol PDHB
Entrez Gene ID 5162
Full Name pyruvate dehydrogenase (lipoamide) beta
Synonyms PDHBD, PDHE1-B, PHE1B
General protein information
Preferred Names
pyruvate dehydrogenase E1 component subunit beta, mitochondrial
Names
pyruvate dehydrogenase E1 component subunit beta, mitochondrial
pyruvate dehydrogenase, E1 beta polypeptide
NP_000916.2
NP_001166939.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

3

3p21.1-p14.2

Summary The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and carbon dioxide, and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 beta subunit. Mutations in this gene are associated with pyruvate dehydrogenase E1-beta deficiency. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2012]. lac of sum
Disorder MIM:

179060

Disorder Html: Pyruvate dehydrogenase E1-beta deficiency (3)

mRNA and Protein(s)

mRNA Protein Name
XM_011533828 XP_011532130 pyruvate dehydrogenase E1 component subunit beta, mitochondrial isoform X1
NM_000925 NP_000916 pyruvate dehydrogenase E1 component subunit beta, mitochondrial isoform 1 precursor
NM_001173468 NP_001166939 pyruvate dehydrogenase E1 component subunit beta, mitochondrial isoform 2 precursor



Homo sapiens (human) PDHB NP_000916.2
Pan troglodytes (chimpanzee) PDHB NP_001233347.1
Macaca mulatta (Rhesus monkey) PDHB XP_001096445.1
Canis lupus familiaris (dog) PDHB XP_533778.2
Bos taurus (cattle) PDHB NP_001030512.2
Mus musculus (house mouse) Pdhb NP_077183.1
Rattus norvegicus (Norway rat) Pdhb NP_001007621.1
Gallus gallus (chicken) PDHB NP_001185549.1
Danio rerio (zebrafish) pdhb NP_998319.1
Drosophila melanogaster (fruit fly) CG11876 NP_651668.1
Caenorhabditis elegans pdhb-1 NP_500340.1
Arabidopsis thaliana (thale cress) MAB1 NP_199898.1
Xenopus (Silurana) tropicalis (western clawed frog) pdhb NP_001016212.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PDHB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDHB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu59601 XM_011533828 PREDICTED: Homo sapiens pyruvate dehydrogenase (lipoamide) beta (PDHB), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu18263
NM_000925 Homo sapiens pyruvate dehydrogenase (lipoamide) beta (PDHB), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu09402 NM_001173468 Homo sapiens pyruvate dehydrogenase (lipoamide) beta (PDHB), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $202.30
$289.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu59601
Clone ID Related Accession (Same CDS sequence) XM_011533828
Accession Version XM_011533828.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1026bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product pyruvate dehydrogenase E1 component subunit beta, mitochondrial isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022517.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCGGCGG TGTCTGGCTT GGTGCGGAGA CCCCTTCGGG AGGTGACAGT TCGTGATGCT 
ATAAATCAGG GTATGGATGA GGAGCTGGAA AGAGATGAGA AGGTATTTCT GCTTGGAGAA
GAAGTTGCCC AGTATGATGG GGCATACAAG GTTAGTCGAG GGCTGTGGAA GAAATATGGA
GACAAGAGGA TTATTGACAC TCCCATATCA GAGATGGGCT TTGCTGGAAT TGCTGTAGGT
GCAGCTATGG CTGGGTTGCG GCCCATTTGT GAATTTATGA CCTTCAATTT CTCCATGCAA
GCCATTGACC AGGTTATAAA CTCAGCTGCC AAGACCTACT ACATGTCTGG TGGCCTTCAG
CCTGTGCCTA TAGTCTTCAG AGGGCCCAAT GGTGCCTCAG CAGGTGTAGC TGCCCAGCAC
TCACAGTGCT TTGCTGCCTG GTATGGGCAC TGCCCAGGCT TAAAGGTGGT CAGTCCCTGG
AATTCAGAGG ATGCTAAAGG ACTTATTAAA TCAGCCATTC GGGATAACAA TCCAGTGGTG
GTGCTAGAGA ATGAATTGAT GTATGGGGTT CCTTTTGAAT TTCCTCCGGA AGCTCAGTCA
AAAGATTTTC TGATTCCTAT TGGAAAAGCC AAAATAGAAA GGCAAGGAAC ACATATAACT
GTGGTTTCCC ATTCAAGACC TGTGGGCCAC TGCTTAGAAG CTGCAGCAGT GCTATCTAAA
GAAGGAGTTG AATGTGAGGT GATAAATATG CGTACCATTA GACCAATGGA CATGGAAACC
ATAGAAGCCA GTGTCATGAA GACAAATCAT CTTGTAACTG TGGAAGGAGG CTGGCCACAG
TTTGGAGTAG GAGCTGAAAT CTGTGCCAGG ATCATGGAAG GTCCTGCGTT CAATTTCCTG
GATGCTCCTG CTGTTCGTGT CACTGGTGCT GATGTCCCTA TGCCTTATGC AAAGATTCTA
GAGGACAACT CTATACCTCA GGTCAAAGAC ATCATATTTG CAATAAAGAA AACATTAAAT
ATTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011532130.1
CDS31..1056
Misc Feature(1)67..1044(+)
Misc Feature(2)85..585(+)
Misc Feature(3)127..561(+)
Misc Feature(4)148..330(+)
Misc Feature(5)151..486(+)
Misc Feature(6)652..1020(+)
Translation

Target ORF information:

RefSeq Version XM_011533828
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens pyruvate dehydrogenase (lipoamide) beta (PDHB), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011533828

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCGGCGG TGTCTGGCTT GGTGCGGAGA CCCCTTCGGG AGGTGACAGT TCGTGATGCT 
ATAAATCAGG GTATGGATGA GGAGCTGGAA AGAGATGAGA AGGTATTTCT GCTTGGAGAA
GAAGTTGCCC AGTATGATGG GGCATACAAG GTTAGTCGAG GGCTGTGGAA GAAATATGGA
GACAAGAGGA TTATTGACAC TCCCATATCA GAGATGGGCT TTGCTGGAAT TGCTGTAGGT
GCAGCTATGG CTGGGTTGCG GCCCATTTGT GAATTTATGA CCTTCAATTT CTCCATGCAA
GCCATTGACC AGGTTATAAA CTCAGCTGCC AAGACCTACT ACATGTCTGG TGGCCTTCAG
CCTGTGCCTA TAGTCTTCAG AGGGCCCAAT GGTGCCTCAG CAGGTGTAGC TGCCCAGCAC
TCACAGTGCT TTGCTGCCTG GTATGGGCAC TGCCCAGGCT TAAAGGTGGT CAGTCCCTGG
AATTCAGAGG ATGCTAAAGG ACTTATTAAA TCAGCCATTC GGGATAACAA TCCAGTGGTG
GTGCTAGAGA ATGAATTGAT GTATGGGGTT CCTTTTGAAT TTCCTCCGGA AGCTCAGTCA
AAAGATTTTC TGATTCCTAT TGGAAAAGCC AAAATAGAAA GGCAAGGAAC ACATATAACT
GTGGTTTCCC ATTCAAGACC TGTGGGCCAC TGCTTAGAAG CTGCAGCAGT GCTATCTAAA
GAAGGAGTTG AATGTGAGGT GATAAATATG CGTACCATTA GACCAATGGA CATGGAAACC
ATAGAAGCCA GTGTCATGAA GACAAATCAT CTTGTAACTG TGGAAGGAGG CTGGCCACAG
TTTGGAGTAG GAGCTGAAAT CTGTGCCAGG ATCATGGAAG GTCCTGCGTT CAATTTCCTG
GATGCTCCTG CTGTTCGTGT CACTGGTGCT GATGTCCCTA TGCCTTATGC AAAGATTCTA
GAGGACAACT CTATACCTCA GGTCAAAGAC ATCATATTTG CAATAAAGAA AACATTAAAT
ATTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18263
Clone ID Related Accession (Same CDS sequence) NM_000925
Accession Version NM_000925.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1080bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product pyruvate dehydrogenase E1 component subunit beta, mitochondrial isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA965753.1, AK225569.1, AK293153.1 and BM971427.1. This sequence is a reference standard in the RefSeqGene project. On Mar 16, 2010 this sequence version replaced gi:156564402. Transcript Variant: This variant (1) represents the predominant transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AK225621.1, J03576.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2146411 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCGGCGG TGTCTGGCTT GGTGCGGAGA CCCCTTCGGG AGGTCTCCGG GCTGCTGAAG 
AGGCGCTTTC ACTGGACCGC GCCGGCTGCG CTGCAGGTGA CAGTTCGTGA TGCTATAAAT
CAGGGTATGG ATGAGGAGCT GGAAAGAGAT GAGAAGGTAT TTCTGCTTGG AGAAGAAGTT
GCCCAGTATG ATGGGGCATA CAAGGTTAGT CGAGGGCTGT GGAAGAAATA TGGAGACAAG
AGGATTATTG ACACTCCCAT ATCAGAGATG GGCTTTGCTG GAATTGCTGT AGGTGCAGCT
ATGGCTGGGT TGCGGCCCAT TTGTGAATTT ATGACCTTCA ATTTCTCCAT GCAAGCCATT
GACCAGGTTA TAAACTCAGC TGCCAAGACC TACTACATGT CTGGTGGCCT TCAGCCTGTG
CCTATAGTCT TCAGGGGGCC CAATGGTGCC TCAGCAGGTG TAGCTGCCCA GCACTCACAG
TGCTTTGCTG CCTGGTATGG GCACTGCCCA GGCTTAAAGG TGGTCAGTCC CTGGAATTCA
GAGGATGCTA AAGGACTTAT TAAATCAGCC ATTCGGGATA ACAATCCAGT GGTGGTGCTA
GAGAATGAAT TGATGTATGG GGTTCCTTTT GAATTTCCTC CGGAAGCTCA GTCAAAAGAT
TTTCTGATTC CTATTGGAAA AGCCAAAATA GAAAGGCAAG GAACACATAT AACTGTGGTT
TCCCATTCAA GACCTGTGGG CCACTGCTTA GAAGCTGCAG CAGTGCTATC TAAAGAAGGA
GTTGAATGTG AGGTGATAAA TATGCGTACC ATTAGACCAA TGGACATGGA AACCATAGAA
GCCAGTGTCA TGAAGACAAA TCATCTTGTA ACTGTGGAAG GAGGCTGGCC ACAGTTTGGA
GTAGGAGCTG AAATCTGTGC CAGGATCATG GAAGGTCCTG CGTTCAATTT CCTGGATGCT
CCTGCTGTTC GTGTCACTGG TGCTGATGTC CCTATGCCTT ATGCAAAGAT TCTAGAGGAC
AACTCTATAC CTCAGGTCAA AGACATCATA TTTGCAATAA AGAAAACATT AAATATTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000916.2
CDS44..1123
Misc Feature(1)59..1111(+)
Misc Feature(2)152..652(+)
Misc Feature(3)194..628(+)
Misc Feature(4)215..397(+)
Misc Feature(5)218..553(+)
Misc Feature(6)719..1087(+)
Exon (1)1..85
Gene:PDHB
Gene Synonym:
Exon (2)86..139
Gene:PDHB
Gene Synonym:
Exon (3)140..247
Gene:PDHB
Gene Synonym:
Exon (4)248..310
Gene:PDHB
Gene Synonym:
Exon (5)311..346
Gene:PDHB
Gene Synonym:
Exon (6)347..632
Gene:PDHB
Gene Synonym:
Exon (7)633..743
Gene:PDHB
Gene Synonym:
Exon (8)744..835
Gene:PDHB
Gene Synonym:
Exon (9)836..977
Gene:PDHB
Gene Synonym:
Exon (10)978..1527
Gene:PDHB
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000925
Organism Homo sapiens (human)
Definition Homo sapiens pyruvate dehydrogenase (lipoamide) beta (PDHB), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000925

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCGGCGG TGTCTGGCTT GGTGCGGAGA CCCCTTCGGG AGGTCTCCGG GCTGCTGAAG 
AGGCGCTTTC ACTGGACCGC GCCGGCTGCG CTGCAGGTGA CAGTTCGTGA TGCTATAAAT
CAGGGTATGG ATGAGGAGCT GGAAAGAGAT GAGAAGGTAT TTCTGCTTGG AGAAGAAGTT
GCCCAGTATG ATGGGGCATA CAAGGTTAGT CGAGGGCTGT GGAAGAAATA TGGAGACAAG
AGGATTATTG ACACTCCCAT ATCAGAGATG GGCTTTGCTG GAATTGCTGT AGGTGCAGCT
ATGGCTGGGT TGCGGCCCAT TTGTGAATTT ATGACCTTCA ATTTCTCCAT GCAAGCCATT
GACCAGGTTA TAAACTCAGC TGCCAAGACC TACTACATGT CTGGTGGCCT TCAGCCTGTG
CCTATAGTCT TCAGGGGGCC CAATGGTGCC TCAGCAGGTG TAGCTGCCCA GCACTCACAG
TGCTTTGCTG CCTGGTATGG GCACTGCCCA GGCTTAAAGG TGGTCAGTCC CTGGAATTCA
GAGGATGCTA AAGGACTTAT TAAATCAGCC ATTCGGGATA ACAATCCAGT GGTGGTGCTA
GAGAATGAAT TGATGTATGG GGTTCCTTTT GAATTTCCTC CGGAAGCTCA GTCAAAAGAT
TTTCTGATTC CTATTGGAAA AGCCAAAATA GAAAGGCAAG GAACACATAT AACTGTGGTT
TCCCATTCAA GACCTGTGGG CCACTGCTTA GAAGCTGCAG CAGTGCTATC TAAAGAAGGA
GTTGAATGTG AGGTGATAAA TATGCGTACC ATTAGACCAA TGGACATGGA AACCATAGAA
GCCAGTGTCA TGAAGACAAA TCATCTTGTA ACTGTGGAAG GAGGCTGGCC ACAGTTTGGA
GTAGGAGCTG AAATCTGTGC CAGGATCATG GAAGGTCCTG CGTTCAATTT CCTGGATGCT
CCTGCTGTTC GTGTCACTGG TGCTGATGTC CCTATGCCTT ATGCAAAGAT TCTAGAGGAC
AACTCTATAC CTCAGGTCAA AGACATCATA TTTGCAATAA AGAAAACATT AAATATTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu09402
Clone ID Related Accession (Same CDS sequence) NM_001173468
Accession Version NM_001173468.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1026bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product pyruvate dehydrogenase E1 component subunit beta, mitochondrial isoform 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA965753.1, AK293153.1 and BM971427.1. Transcript Variant: This variant (2) lacks a segment in the coding region compared to variant 1. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AK293153.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1970526, SAMEA2146411 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCGGCGG TGTCTGGCTT GGTGCGGAGA CCCCTTCGGG AGGTCTCCGG GCTGCTGAAG 
AGGCGCTTTC ACTGGACCGC GCCGGCTGCG CTGCAGGTGA CAGTTCGTGA TGCTATAAAT
CAGGGTATGG ATGAGGAGCT GGAAAGAGAT GAGAAGGTAT TTCTGCTTGG AGAAGAAGTT
GCCCAGTATG ATGGGGCATA CAAGGTTAGT CGAGGGCTGT GGAAGAAATA TGGAGACAAG
AGGATTATTG ACACTCCCAT ATCAGAGATG GGCTTTGCTG GAATTGCTGT AGGTGCAGCT
ATGGCTGGGT TGCGGCCCAT TTGTGAATTT ATGACCTTCA ATTTCTCCAT GCAAGCCATT
GACCAGGTTA TAAACTCAGC TGCCAAGACC TACTACATGT CTGGTGTAGC TGCCCAGCAC
TCACAGTGCT TTGCTGCCTG GTATGGGCAC TGCCCAGGCT TAAAGGTGGT CAGTCCCTGG
AATTCAGAGG ATGCTAAAGG ACTTATTAAA TCAGCCATTC GGGATAACAA TCCAGTGGTG
GTGCTAGAGA ATGAATTGAT GTATGGGGTT CCTTTTGAAT TTCCTCCGGA AGCTCAGTCA
AAAGATTTTC TGATTCCTAT TGGAAAAGCC AAAATAGAAA GGCAAGGAAC ACATATAACT
GTGGTTTCCC ATTCAAGACC TGTGGGCCAC TGCTTAGAAG CTGCAGCAGT GCTATCTAAA
GAAGGAGTTG AATGTGAGGT GATAAATATG CGTACCATTA GACCAATGGA CATGGAAACC
ATAGAAGCCA GTGTCATGAA GACAAATCAT CTTGTAACTG TGGAAGGAGG CTGGCCACAG
TTTGGAGTAG GAGCTGAAAT CTGTGCCAGG ATCATGGAAG GTCCTGCGTT CAATTTCCTG
GATGCTCCTG CTGTTCGTGT CACTGGTGCT GATGTCCCTA TGCCTTATGC AAAGATTCTA
GAGGACAACT CTATACCTCA GGTCAAAGAC ATCATATTTG CAATAAAGAA AACATTAAAT
ATTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001166939.1
CDS44..1069
Misc Feature(1)59..1057(+)
Misc Feature(2)152..598(+)
Misc Feature(3)194..574(+)
Misc Feature(4)215..397(+)
Misc Feature(5)218..499(+)
Misc Feature(6)665..1033(+)
Exon (1)1..85
Gene:PDHB
Gene Synonym:
Exon (2)86..139
Gene:PDHB
Gene Synonym:
Exon (3)140..247
Gene:PDHB
Gene Synonym:
Exon (4)248..310
Gene:PDHB
Gene Synonym:
Exon (5)311..346
Gene:PDHB
Gene Synonym:
Exon (6)347..446
Gene:PDHB
Gene Synonym:
Exon (7)447..578
Gene:PDHB
Gene Synonym:
Exon (8)579..689
Gene:PDHB
Gene Synonym:
Exon (9)690..781
Gene:PDHB
Gene Synonym:
Exon (10)782..923
Gene:PDHB
Gene Synonym:
Exon (11)924..1473
Gene:PDHB
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001173468
Organism Homo sapiens (human)
Definition Homo sapiens pyruvate dehydrogenase (lipoamide) beta (PDHB), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001173468

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCGGCGG TGTCTGGCTT GGTGCGGAGA CCCCTTCGGG AGGTCTCCGG GCTGCTGAAG 
AGGCGCTTTC ACTGGACCGC GCCGGCTGCG CTGCAGGTGA CAGTTCGTGA TGCTATAAAT
CAGGGTATGG ATGAGGAGCT GGAAAGAGAT GAGAAGGTAT TTCTGCTTGG AGAAGAAGTT
GCCCAGTATG ATGGGGCATA CAAGGTTAGT CGAGGGCTGT GGAAGAAATA TGGAGACAAG
AGGATTATTG ACACTCCCAT ATCAGAGATG GGCTTTGCTG GAATTGCTGT AGGTGCAGCT
ATGGCTGGGT TGCGGCCCAT TTGTGAATTT ATGACCTTCA ATTTCTCCAT GCAAGCCATT
GACCAGGTTA TAAACTCAGC TGCCAAGACC TACTACATGT CTGGTGTAGC TGCCCAGCAC
TCACAGTGCT TTGCTGCCTG GTATGGGCAC TGCCCAGGCT TAAAGGTGGT CAGTCCCTGG
AATTCAGAGG ATGCTAAAGG ACTTATTAAA TCAGCCATTC GGGATAACAA TCCAGTGGTG
GTGCTAGAGA ATGAATTGAT GTATGGGGTT CCTTTTGAAT TTCCTCCGGA AGCTCAGTCA
AAAGATTTTC TGATTCCTAT TGGAAAAGCC AAAATAGAAA GGCAAGGAAC ACATATAACT
GTGGTTTCCC ATTCAAGACC TGTGGGCCAC TGCTTAGAAG CTGCAGCAGT GCTATCTAAA
GAAGGAGTTG AATGTGAGGT GATAAATATG CGTACCATTA GACCAATGGA CATGGAAACC
ATAGAAGCCA GTGTCATGAA GACAAATCAT CTTGTAACTG TGGAAGGAGG CTGGCCACAG
TTTGGAGTAG GAGCTGAAAT CTGTGCCAGG ATCATGGAAG GTCCTGCGTT CAATTTCCTG
GATGCTCCTG CTGTTCGTGT CACTGGTGCT GATGTCCCTA TGCCTTATGC AAAGATTCTA
GAGGACAACT CTATACCTCA GGTCAAAGAC ATCATATTTG CAATAAAGAA AACATTAAAT
ATTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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