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CNGB3 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol CNGB3
Entrez Gene ID 54714
Full Name cyclic nucleotide gated channel beta 3
Synonyms ACHM1
General protein information
Preferred Names

cyclic nucleotide gated channel beta 3

Names

cyclic nucleotide-gated cation channel beta-3
CNG channel beta-3
cone photoreceptor cGMP-gated cation channel beta-subunit
cyclic nucleotide-gated cation channel modulatory subunit

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

8

8q21.3

Summary This gene encodes the beta subunit of a cyclic nucleotide-gated ion channel. The encoded beta subunit appears to play a role in modulation of channel function in cone photoreceptors. This heterotetrameric channel is necessary for sensory transduction, and mutations in this gene have been associated with achromatopsia 3, progressive cone dystrophy, and juvenile macular degeneration, also known as Stargardt Disease. [provided by RefSeq, Feb 2010].
Disorder MIM:

605080

Disorder Html: Achromatopsia-3, 262300 (3); Macular degeneration, juvenile, 248200

mRNA and Protein(s)

mRNA Protein Name
XM_011517138.2 XP_011515440.1 cyclic nucleotide-gated cation channel beta-3 isoform X1
NM_019098.4 NP_061971.3 cyclic nucleotide-gated cation channel beta-3



Pan troglodytes (chimpanzee) CNGB3 XP_519846.2
Macaca mulatta (rhesus monkey) CNGB3 XP_001083646.1
Homo sapiens (human) CNGB3 NP_061971.3
Bos taurus (cattle) CNGB3 XP_002692873.2
Danio rerio (zebrafish) cngb3 XP_691142.4
Canis lupus familiaris (dog) CNGB3 NP_001003030.1
Rattus norvegicus (Norway rat) Cngb3 NP_001258167.1
Danio rerio (zebrafish) LOC562466 XP_690937.4
Xenopus tropicalis (tropical clawed frog) cngb3 XP_002939202.2
Mus musculus (house mouse) Cngb3 NP_038955.1
Gallus gallus (chicken) CNGB3 XP_425928.4


Related articles in PubMed

Retinal morphology of patients with achromatopsia during early childhood: implications for gene therapy.
Yang P, Michaels KV, Courtney RJ, Wen Y, Greninger DA, Reznick L, Karr DJ, Wilson LB, Weleber RG, Pennesi ME
JAMA ophthalmology132(7)823-31(2014 Jul)

Five novel CNGB3 gene mutations in Polish patients with achromatopsia.
Wawrocka A, Kohl S, Baumann B, Walczak-Sztulpa J, Wicher K, Skorczyk-Werner A, Krawczynski MR
Molecular vision201732-9(2014)

Spectral domain optical coherence tomography findings in CNGB3-associated achromatopsia and therapeutic implications.
McClintock M, Peden MC, Kay CN
Advances in experimental medicine and biology801551-7(2014)

Cyclic nucleotide-gated channel subunit glycosylation regulates matrix metalloproteinase-dependent changes in channel gating.
Meighan SE, Meighan PC, Rich ED, Brown RL, Varnum MD
Biochemistry52(46)8352-62(2013 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The degree of residual foveal cone structure varies greatly among subjects with CNGB3-associated ACHM.
Title: Residual Foveal Cone Structure in CNGB3-Associated Achromatopsia.

Genetic testing revealed a common homozygous mutation in CNGB3 in 5 patients with complete achromatopsia and heterozygous mutations in CNGA3 in 2 patients with incomplete achromatopsia.
Title: Retinal morphology of patients with achromatopsia during early childhood: implications for gene therapy.

Eight different mutations were detected in the CNGB3 gene in achromatopsia, including five novel mutations: two splice site mutations, one nonsense substitution, and two frame-shift mutations.
Title: Five novel CNGB3 gene mutations in Polish patients with achromatopsia.

Genetic testing performed at Carver lab at the University of Iowa confirmed a diagnosis of achromatopsia with identical mutations in the CNGB3 gene.
Title: Spectral domain optical coherence tomography findings in CNGB3-associated achromatopsia and therapeutic implications.

Data found pathogenic DNA variants in the genes RP1, USH2A, CNGB3, NMNAT1, CHM, and ABCA4, responsible for retinitis pigmentosa, Usher syndrome, achromatopsia, Leber congenital amaurosis, choroideremia, or recessive Stargardt/cone-rod dystrophy cases.
Title: Exome sequencing of index patients with retinal dystrophies as a tool for molecular diagnosis.

The following CNGB3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CNGB3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu18723 NM_019098.4
Latest version!
Homo sapiens cyclic nucleotide gated channel beta 3 (CNGB3), mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $265.30
$379.00
OHu68494 XM_011517138.2
Latest version!
Homo sapiens cyclic nucleotide gated channel beta 3 (CNGB3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $391.30
$559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu18723
Clone ID Related Accession (Same CDS sequence) NM_019098.4
Accession Version NM_019098.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2430bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-09-09
Organism Homo sapiens(Human)
Product cyclic nucleotide-gated cation channel beta-3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL713036.1, AF272900.1, AF228520.1 and BX118844.1. This sequence is a reference standard in the RefSeqGene project. On Feb 19, 2010 this sequence version replaced NM_019098.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF272900.1, BC150601.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968189, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGTTTAAAT CGCTGACAAA AGTCAACAAG GTGAAGCCTA TAGGAGAGAA CAATGAGAAT 
GAACAAAGTT CTCGTCGGAA TGAAGAAGGC TCTCACCCAA GTAATCAGTC TCAGCAAACC
ACAGCACAGG AAGAAAACAA AGGTGAAGAG AAATCTCTCA AAACCAAGTC AACTCCAGTC
ACGTCTGAAG AGCCACACAC CAACATACAA GACAAACTCT CCAAGAAAAA TTCCTCTGGA
GATCTGACCA CAAACCCTGA CCCTCAAAAT GCAGCAGAAC CAACTGGAAC AGTGCCAGAG
CAGAAGGAAA TGGACCCCGG GAAAGAAGGT CCAAACAGCC CACAAAACAA ACCGCCTGCA
GCTCCTGTTA TAAATGAGTA TGCCGATGCC CAGCTACACA ACCTGGTGAA AAGAATGCGT
CAAAGAACAG CCCTCTACAA GAAAAAGTTG GTAGAGGGAG ATCTCTCCTC ACCCGAAGCC
AGCCCACAAA CTGCAAAGCC CACGGCTGTA CCACCAGTAA AAGAAAGCGA TGATAAGCCA
ACAGAACATT ACTACAGGCT GTTGTGGTTC AAAGTCAAAA AGATGCCTTT AACAGAGTAC
TTAAAGCGAA TTAAACTTCC AAACAGCATA GATTCATACA CAGATCGACT CTATCTCCTG
TGGCTCTTGC TTGTCACTCT TGCCTATAAC TGGAACTGCT GTTTTATACC ACTGCGCCTC
GTCTTCCCAT ATCAAACCGC AGACAACATA CACTACTGGC TTATTGCGGA CATCATATGT
GATATCATCT ACCTTTATGA TATGCTATTT ATCCAGCCCA GACTCCAGTT TGTAAGAGGA
GGAGACATAA TAGTGGATTC AAATGAGCTA AGGAAACACT ACAGGACTTC TACAAAATTT
CAGTTGGATG TCGCATCAAT AATACCATTT GATATTTGCT ACCTCTTCTT TGGGTTTAAT
CCAATGTTTA GAGCAAATAG GATGTTAAAG TACACTTCAT TTTTTGAATT TAATCATCAC
CTAGAGTCTA TAATGGACAA AGCATATATC TACAGAGTTA TTCGAACAAC TGGATACTTG
CTGTTTATTC TGCACATTAA TGCCTGTGTT TATTACTGGG CTTCAAACTA TGAAGGAATT
GGCACTACTA GATGGGTGTA TGATGGGGAA GGAAACGAGT ATCTGAGATG TTATTATTGG
GCAGTTCGAA CTTTAATTAC CATTGGTGGC CTTCCAGAAC CACAAACTTT ATTTGAAATT
GTTTTTCAAC TCTTGAATTT TTTTTCTGGA GTTTTTGTGT TCTCCAGTTT AATTGGTCAG
ATGAGAGATG TGATTGGAGC AGCTACAGCC AATCAGAACT ACTTCCGCGC CTGCATGGAT
GACACCATTG CCTACATGAA CAATTACTCC ATTCCTAAAC TTGTGCAAAA GCGAGTTCGG
ACTTGGTATG AATATACATG GGACTCTCAA AGAATGCTAG ATGAGTCTGA TTTGCTTAAG
ACCCTACCAA CTACGGTCCA GTTAGCCCTC GCCATTGATG TGAACTTCAG CATCATCAGC
AAAGTCGACT TGTTCAAGGG TTGTGATACA CAGATGATTT ATGACATGTT GCTAAGATTG
AAATCCGTTC TCTATTTGCC TGGTGACTTT GTCTGCAAAA AGGGAGAAAT TGGCAAGGAA
ATGTATATCA TCAAGCATGG AGAAGTCCAA GTTCTTGGAG GCCCTGATGG TACTAAAGTT
CTGGTTACTC TGAAAGCTGG GTCGGTGTTT GGAGAAATCA GCCTTCTAGC AGCAGGAGGA
GGAAACCGTC GAACTGCCAA TGTGGTGGCC CACGGGTTTG CCAATCTTTT AACTCTAGAC
AAAAAGACCC TCCAAGAAAT TCTAGTGCAT TATCCAGATT CTGAAAGGAT CCTCATGAAG
AAAGCCAGAG TGCTTTTAAA GCAGAAGGCT AAGACCGCAG AAGCAACCCC TCCAAGAAAA
GATCTTGCCC TCCTCTTCCC ACCGAAAGAA GAGACACCCA AACTGTTTAA AACTCTCCTA
GGAGGCACAG GAAAAGCAAG TCTTGCAAGA CTACTCAAAT TGAAGCGAGA GCAAGCAGCT
CAGAAGAAAG AAAATTCTGA AGGAGGAGAG GAAGAAGGAA AAGAAAATGA AGATAAACAA
AAAGAAAATG AAGATAAACA AAAAGAAAAT GAAGATAAAG GAAAAGAAAA TGAAGATAAA
GATAAAGGAA GAGAGCCAGA AGAGAAGCCA CTGGACAGAC CTGAATGTAC AGCAAGTCCT
ATTGCAGTGG AGGAAGAACC CCACTCAGTT AGAAGGACAG TTTTACCCAG AGGGACTTCT
CGTCAATCAC TCATTATCAG CATGGCTCCT TCTGCTGAGG GCGGAGAAGA GGTTCTTACT
ATTGAAGTCA AAGAAAAGGC TAAGCAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_061971.3
CDS49..2478
Misc Feature(1)13..15
Misc Feature(2)697..759
Misc Feature(3)760..1407
Misc Feature(4)799..861
Misc Feature(5)955..1017
Misc Feature(6)1126..1188
Misc Feature(7)1300..1362
Misc Feature(8)1561..1623
Misc Feature(9)1609..>1992
Misc Feature(10)1618..1965
Misc Feature(11)order(1819..1824,1858..1866)
Misc Feature(12)order(1924..1932,1942..1950)
Exon (1)1..177
Exon (2)178..259
Exon (3)260..386
Exon (4)387..541
Exon (5)542..691
Exon (6)692..900
Exon (7)901..951
Exon (8)952..1038
Exon (9)1039..1103
Exon (10)1104..1226
Exon (11)1227..1368
Exon (12)1369..1528
Exon (13)1529..1626
Exon (14)1627..1710
Exon (15)1711..1829
Exon (16)1830..1976
Exon (17)1977..2151
Exon (18)2152..4347
Translation

Target ORF information:

RefSeq Version NM_019098.4
Organism Homo sapiens(Human)
Definition Homo sapiens cyclic nucleotide gated channel beta 3 (CNGB3), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_019098.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGTTTAAAT CGCTGACAAA AGTCAACAAG GTGAAGCCTA TAGGAGAGAA CAATGAGAAT 
GAACAAAGTT CTCGTCGGAA TGAAGAAGGC TCTCACCCAA GTAATCAGTC TCAGCAAACC
ACAGCACAGG AAGAAAACAA AGGTGAAGAG AAATCTCTCA AAACCAAGTC AACTCCAGTC
ACGTCTGAAG AGCCACACAC CAACATACAA GACAAACTCT CCAAGAAAAA TTCCTCTGGA
GATCTGACCA CAAACCCTGA CCCTCAAAAT GCAGCAGAAC CAACTGGAAC AGTGCCAGAG
CAGAAGGAAA TGGACCCCGG GAAAGAAGGT CCAAACAGCC CACAAAACAA ACCGCCTGCA
GCTCCTGTTA TAAATGAGTA TGCCGATGCC CAGCTACACA ACCTGGTGAA AAGAATGCGT
CAAAGAACAG CCCTCTACAA GAAAAAGTTG GTAGAGGGAG ATCTCTCCTC ACCCGAAGCC
AGCCCACAAA CTGCAAAGCC CACGGCTGTA CCACCAGTAA AAGAAAGCGA TGATAAGCCA
ACAGAACATT ACTACAGGCT GTTGTGGTTC AAAGTCAAAA AGATGCCTTT AACAGAGTAC
TTAAAGCGAA TTAAACTTCC AAACAGCATA GATTCATACA CAGATCGACT CTATCTCCTG
TGGCTCTTGC TTGTCACTCT TGCCTATAAC TGGAACTGCT GTTTTATACC ACTGCGCCTC
GTCTTCCCAT ATCAAACCGC AGACAACATA CACTACTGGC TTATTGCGGA CATCATATGT
GATATCATCT ACCTTTATGA TATGCTATTT ATCCAGCCCA GACTCCAGTT TGTAAGAGGA
GGAGACATAA TAGTGGATTC AAATGAGCTA AGGAAACACT ACAGGACTTC TACAAAATTT
CAGTTGGATG TCGCATCAAT AATACCATTT GATATTTGCT ACCTCTTCTT TGGGTTTAAT
CCAATGTTTA GAGCAAATAG GATGTTAAAG TACACTTCAT TTTTTGAATT TAATCATCAC
CTAGAGTCTA TAATGGACAA AGCATATATC TACAGAGTTA TTCGAACAAC TGGATACTTG
CTGTTTATTC TGCACATTAA TGCCTGTGTT TATTACTGGG CTTCAAACTA TGAAGGAATT
GGCACTACTA GATGGGTGTA TGATGGGGAA GGAAACGAGT ATCTGAGATG TTATTATTGG
GCAGTTCGAA CTTTAATTAC CATTGGTGGC CTTCCAGAAC CACAAACTTT ATTTGAAATT
GTTTTTCAAC TCTTGAATTT TTTTTCTGGA GTTTTTGTGT TCTCCAGTTT AATTGGTCAG
ATGAGAGATG TGATTGGAGC AGCTACAGCC AATCAGAACT ACTTCCGCGC CTGCATGGAT
GACACCATTG CCTACATGAA CAATTACTCC ATTCCTAAAC TTGTGCAAAA GCGAGTTCGG
ACTTGGTATG AATATACATG GGACTCTCAA AGAATGCTAG ATGAGTCTGA TTTGCTTAAG
ACCCTACCAA CTACGGTCCA GTTAGCCCTC GCCATTGATG TGAACTTCAG CATCATCAGC
AAAGTCGACT TGTTCAAGGG TTGTGATACA CAGATGATTT ATGACATGTT GCTAAGATTG
AAATCCGTTC TCTATTTGCC TGGTGACTTT GTCTGCAAAA AGGGAGAAAT TGGCAAGGAA
ATGTATATCA TCAAGCATGG AGAAGTCCAA GTTCTTGGAG GCCCTGATGG TACTAAAGTT
CTGGTTACTC TGAAAGCTGG GTCGGTGTTT GGAGAAATCA GCCTTCTAGC AGCAGGAGGA
GGAAACCGTC GAACTGCCAA TGTGGTGGCC CACGGGTTTG CCAATCTTTT AACTCTAGAC
AAAAAGACCC TCCAAGAAAT TCTAGTGCAT TATCCAGATT CTGAAAGGAT CCTCATGAAG
AAAGCCAGAG TGCTTTTAAA GCAGAAGGCT AAGACCGCAG AAGCAACCCC TCCAAGAAAA
GATCTTGCCC TCCTCTTCCC ACCGAAAGAA GAGACACCCA AACTGTTTAA AACTCTCCTA
GGAGGCACAG GAAAAGCAAG TCTTGCAAGA CTACTCAAAT TGAAGCGAGA GCAAGCAGCT
CAGAAGAAAG AAAATTCTGA AGGAGGAGAG GAAGAAGGAA AAGAAAATGA AGATAAACAA
AAAGAAAATG AAGATAAACA AAAAGAAAAT GAAGATAAAG GAAAAGAAAA TGAAGATAAA
GATAAAGGAA GAGAGCCAGA AGAGAAGCCA CTGGACAGAC CTGAATGTAC AGCAAGTCCT
ATTGCAGTGG AGGAAGAACC CCACTCAGTT AGAAGGACAG TTTTACCCAG AGGGACTTCT
CGTCAATCAC TCATTATCAG CATGGCTCCT TCTGCTGAGG GCGGAGAAGA GGTTCTTACT
ATTGAAGTCA AAGAAAAGGC TAAGCAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu68494
Clone ID Related Accession (Same CDS sequence) XM_011517138.2
Accession Version XM_011517138.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2016bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product cyclic nucleotide-gated cation channel beta-3 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011517138.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGCGTCAAA GAACAGCCCT CTACAAGAAA AAGTTGGTAG AGGGAGATCT CTCCTCACCC 
GAAGCCAGCC CACAAACTGC AAAGCCCACG GCTGTACCAC CAGTAAAAGA AAGCGATGAT
AAGCCAACAG AACATTACTA CAGGCTGTTG TGGTTCAAAG TCAAAAAGAT GCCTTTAACA
GAGTACTTAA AGCGAATTAA ACTTCCAAAC AGCATAGATT CATACACAGA TCGACTCTAT
CTCCTGTGGC TCTTGCTTGT CACTCTTGCC TATAACTGGA ACTGCTGTTT TATACCACTG
CGCCTCGTCT TCCCATATCA AACCGCAGAC AACATACACT ACTGGCTTAT TGCGGACATC
ATATGTGATA TCATCTACCT TTATGATATG CTATTTATCC AGCCCAGACT CCAGTTTGTA
AGAGGAGGAG ACATAATAGT GGATTCAAAT GAGCTAAGGA AACACTACAG GACTTCTACA
AAATTTCAGT TGGATGTCGC ATCAATAATA CCATTTGATA TTTGCTACCT CTTCTTTGGG
TTTAATCCAA TGTTTAGAGC AAATAGGATG TTAAAGTACA CTTCATTTTT TGAATTTAAT
CATCACCTAG AGTCTATAAT GGACAAAGCA TATATCTACA GAGTTATTCG AACAACTGGA
TACTTGCTGT TTATTCTGCA CATTAATGCC TGTGTTTATT ACTGGGCTTC AAACTATGAA
GGAATTGGCA CTACTAGATG GGTGTATGAT GGGGAAGGAA ACGAGTATCT GAGATGTTAT
TATTGGGCAG TTCGAACTTT AATTACCATT GGTGGCCTTC CAGAACCACA AACTTTATTT
GAAATTGTTT TTCAACTCTT GAATTTTTTT TCTGGAGTTT TTGTGTTCTC CAGTTTAATT
GGTCAGATGA GAGATGTGAT TGGAGCAGCT ACAGCCAATC AGAACTACTT CCGCGCCTGC
ATGGATGACA CCATTGCCTA CATGAACAAT TACTCCATTC CTAAACTTGT GCAAAAGCGA
GTTCGGACTT GGTATGAATA TACATGGGAC TCTCAAAGAA TGCTAGATGA GTCTGATTTG
CTTAAGACCC TACCAACTAC GGTCCAGTTA GCCCTCGCCA TTGATGTGAA CTTCAGCATC
ATCAGCAAAG TCGACTTGTT CAAGGGTTGT GATACACAGA TGATTTATGA CATGTTGCTA
AGATTGAAAT CCGTTCTCTA TTTGCCTGGT GACTTTGTCT GCAAAAAGGG AGAAATTGGC
AAGGAAATGT ATATCATCAA GCATGGAGAA GTCCAAGTTC TTGGAGGCCC TGATGGTACT
AAAGTTCTGG TTACTCTGAA AGCTGGGTCG GTGTTTGGAG AAATCAGCCT TCTAGCAGCA
GGAGGAGGAA ACCGTCGAAC TGCCAATGTG GTGGCCCACG GGTTTGCCAA TCTTTTAACT
CTAGACAAAA AGACCCTCCA AGAAATTCTA GTGCATTATC CAGATTCTGA AAGGATCCTC
ATGAAGAAAG CCAGAGTGCT TTTAAAGCAG AAGGCTAAGA CCGCAGAAGC AACCCCTCCA
AGAAAAGATC TTGCCCTCCT CTTCCCACCG AAAGAAGAGA CACCCAAACT GTTTAAAACT
CTCCTAGGAG GCACAGGAAA AGCAAGTCTT GCAAGACTAC TCAAATTGAA GCGAGAGCAA
GCAGCTCAGA AGAAAGAAAA TTCTGAAGGA GGAGAGGAAG AAGGAAAAGA AAATGAAGAT
AAACAAAAAG AAAATGAAGA TAAACAAAAA GAAAATGAAG ATAAAGGAAA AGAAAATGAA
GATAAAGATA AAGGAAGAGA GCCAGAAGAG AAGCCACTGG ACAGACCTGA ATGTACAGCA
AGTCCTATTG CAGTGGAGGA AGAACCCCAC TCAGTTAGAA GGACAGTTTT ACCCAGAGGG
ACTTCTCGTC AATCACTCAT TATCAGCATG GCTCCTTCTG CTGAGGGCGG AGAAGAGGTT
CTTACTATTG AAGTCAAAGA AAAGGCTAAG CAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011515440.1
CDS118..2133
Misc Feature(1)415..1062
Misc Feature(2)1264..>1647
Misc Feature(3)1273..1620
Misc Feature(4)order(1474..1479,1513..1521)
Misc Feature(5)order(1579..1587,1597..1605)
Translation

Target ORF information:

RefSeq Version XM_011517138.2
Organism Homo sapiens(Human)
Definition Homo sapiens cyclic nucleotide gated channel beta 3 (CNGB3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011517138.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGCGTCAAA GAACAGCCCT CTACAAGAAA AAGTTGGTAG AGGGAGATCT CTCCTCACCC 
GAAGCCAGCC CACAAACTGC AAAGCCCACG GCTGTACCAC CAGTAAAAGA AAGCGATGAT
AAGCCAACAG AACATTACTA CAGGCTGTTG TGGTTCAAAG TCAAAAAGAT GCCTTTAACA
GAGTACTTAA AGCGAATTAA ACTTCCAAAC AGCATAGATT CATACACAGA TCGACTCTAT
CTCCTGTGGC TCTTGCTTGT CACTCTTGCC TATAACTGGA ACTGCTGTTT TATACCACTG
CGCCTCGTCT TCCCATATCA AACCGCAGAC AACATACACT ACTGGCTTAT TGCGGACATC
ATATGTGATA TCATCTACCT TTATGATATG CTATTTATCC AGCCCAGACT CCAGTTTGTA
AGAGGAGGAG ACATAATAGT GGATTCAAAT GAGCTAAGGA AACACTACAG GACTTCTACA
AAATTTCAGT TGGATGTCGC ATCAATAATA CCATTTGATA TTTGCTACCT CTTCTTTGGG
TTTAATCCAA TGTTTAGAGC AAATAGGATG TTAAAGTACA CTTCATTTTT TGAATTTAAT
CATCACCTAG AGTCTATAAT GGACAAAGCA TATATCTACA GAGTTATTCG AACAACTGGA
TACTTGCTGT TTATTCTGCA CATTAATGCC TGTGTTTATT ACTGGGCTTC AAACTATGAA
GGAATTGGCA CTACTAGATG GGTGTATGAT GGGGAAGGAA ACGAGTATCT GAGATGTTAT
TATTGGGCAG TTCGAACTTT AATTACCATT GGTGGCCTTC CAGAACCACA AACTTTATTT
GAAATTGTTT TTCAACTCTT GAATTTTTTT TCTGGAGTTT TTGTGTTCTC CAGTTTAATT
GGTCAGATGA GAGATGTGAT TGGAGCAGCT ACAGCCAATC AGAACTACTT CCGCGCCTGC
ATGGATGACA CCATTGCCTA CATGAACAAT TACTCCATTC CTAAACTTGT GCAAAAGCGA
GTTCGGACTT GGTATGAATA TACATGGGAC TCTCAAAGAA TGCTAGATGA GTCTGATTTG
CTTAAGACCC TACCAACTAC GGTCCAGTTA GCCCTCGCCA TTGATGTGAA CTTCAGCATC
ATCAGCAAAG TCGACTTGTT CAAGGGTTGT GATACACAGA TGATTTATGA CATGTTGCTA
AGATTGAAAT CCGTTCTCTA TTTGCCTGGT GACTTTGTCT GCAAAAAGGG AGAAATTGGC
AAGGAAATGT ATATCATCAA GCATGGAGAA GTCCAAGTTC TTGGAGGCCC TGATGGTACT
AAAGTTCTGG TTACTCTGAA AGCTGGGTCG GTGTTTGGAG AAATCAGCCT TCTAGCAGCA
GGAGGAGGAA ACCGTCGAAC TGCCAATGTG GTGGCCCACG GGTTTGCCAA TCTTTTAACT
CTAGACAAAA AGACCCTCCA AGAAATTCTA GTGCATTATC CAGATTCTGA AAGGATCCTC
ATGAAGAAAG CCAGAGTGCT TTTAAAGCAG AAGGCTAAGA CCGCAGAAGC AACCCCTCCA
AGAAAAGATC TTGCCCTCCT CTTCCCACCG AAAGAAGAGA CACCCAAACT GTTTAAAACT
CTCCTAGGAG GCACAGGAAA AGCAAGTCTT GCAAGACTAC TCAAATTGAA GCGAGAGCAA
GCAGCTCAGA AGAAAGAAAA TTCTGAAGGA GGAGAGGAAG AAGGAAAAGA AAATGAAGAT
AAACAAAAAG AAAATGAAGA TAAACAAAAA GAAAATGAAG ATAAAGGAAA AGAAAATGAA
GATAAAGATA AAGGAAGAGA GCCAGAAGAG AAGCCACTGG ACAGACCTGA ATGTACAGCA
AGTCCTATTG CAGTGGAGGA AGAACCCCAC TCAGTTAGAA GGACAGTTTT ACCCAGAGGG
ACTTCTCGTC AATCACTCAT TATCAGCATG GCTCCTTCTG CTGAGGGCGG AGAAGAGGTT
CTTACTATTG AAGTCAAAGA AAAGGCTAAG CAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Retinal morphology of patients with achromatopsia during early childhood: implications for gene therapy.
JAMA ophthalmology132(7)823-31(2014 Jul)
Yang P,Michaels KV,Courtney RJ,Wen Y,Greninger DA,Reznick L,Karr DJ,Wilson LB,Weleber RG,Pennesi ME


book

Five novel CNGB3 gene mutations in Polish patients with achromatopsia.
Molecular vision201732-9(2014)
Wawrocka A,Kohl S,Baumann B,Walczak-Sztulpa J,Wicher K,Skorczyk-Werner A,Krawczynski MR


book

Spectral domain optical coherence tomography findings in CNGB3-associated achromatopsia and therapeutic implications.
Advances in experimental medicine and biology801551-7(2014)
McClintock M,Peden MC,Kay CN


book

Cyclic nucleotide-gated channel subunit glycosylation regulates matrix metalloproteinase-dependent changes in channel gating.
Biochemistry52(46)8352-62(2013 Nov)
Meighan SE,Meighan PC,Rich ED,Brown RL,Varnum MD


 
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