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PPIB cDNA ORF clone, Homo sapiens(Human)

Gene Symbol PPIB
Entrez Gene ID 5479
Full Name peptidylprolyl isomerase B
Synonyms CYP-S1,CYPB,HEL-S-39,OI9,SCYLP
General protein information
Preferred Names

peptidylprolyl isomerase B

Names

peptidyl-prolyl cis-trans isomerase B
PPIase B
S-cyclophilin
cyclophilin-like protein
epididymis secretory protein Li 39
peptidylprolyl isomerase B (cyclophilin B)
rotamase B

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

15

15q22.31

Summary The protein encoded by this gene is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Variants have been identified in this protein that give rise to recessive forms of osteogenesis imperfecta. [provided by RefSeq, Oct 2009].
Disorder MIM:

123841

Disorder Html: Osteogenesis imperfecta, type IX, 259440 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_000942.4 NP_000933.1 peptidyl-prolyl cis-trans isomerase B precursor



Canis lupus familiaris (dog) PPIB XP_852389.1
Rattus norvegicus (Norway rat) Ppib NP_071981.1
Danio rerio (zebrafish) ppib NP_998184.1
Saccharomyces cerevisiae (baker's yeast) CPR5 NP_010590.3
Macaca mulatta (rhesus monkey) PPIB XP_001106590.1
Kluyveromyces lactis KLLA0C10208g XP_452654.1
Bos taurus (cattle) PPIB NP_776577.1
Drosophila melanogaster (fruit fly) CG2852 NP_611695.1
Schizosaccharomyces pombe (fission yeast) cyp4 NP_596532.1
Neurospora crassa NCU01200 XP_001728305.1
Homo sapiens (human) PPIB NP_000933.1
Pan troglodytes (chimpanzee) PPIB XP_003952716.1
Mus musculus (house mouse) Ppib NP_035279.2
Gallus gallus (chicken) PPIB NP_990792.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP007088 XP_308669.2
Eremothecium gossypii AGOS_AAL056C NP_982486.1
Arabidopsis thaliana (thale cress) CYP20-2 NP_001190299.1
Caenorhabditis elegans (roundworm) cyn-6 NP_497257.1
Caenorhabditis elegans (roundworm) cyn-5 NP_493624.1
Magnaporthe oryzae (rice blast fungus) MGG_15814 XP_003715036.1
Oryza sativa (rice) Os05g0103200 NP_001054392.1
Xenopus tropicalis (tropical clawed frog) ppib NP_001017063.1


Related articles in PubMed

Cyclophilin B enhances HIV-1 infection.
DeBoer J, Madson CJ, Belshan M
Virology489282-91(2016 Feb)

Mutational characterization of the P3H1/CRTAP/CypB complex in recessive osteogenesis imperfecta.
Barbirato C, Trancozo M, Almeida MG, Almeida LS, Santos TO, Duarte JC, Rebouças MR, Sipolatti V, Nunes VR, Paula F
Genetics and molecular research : GMR14(4)15848-58(2015 Dec)

Expression and role of cyclophilin B in stomach cancer.
Meng DQ, Li PL, Xie M
Genetics and molecular research : GMR14(2)5346-54(2015 May)

Depletion of cyclophilins B and C leads to dysregulation of endoplasmic reticulum redox homeostasis.
Stocki P, Chapman DC, Beach LA, Williams DB
The Journal of biological chemistry289(33)23086-96(2014 Aug)

Functional aspects of extracellular cyclophilins.
Hoffmann H, Schiene-Fischer C
Biological chemistry395(7-8)721-35(2014 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

observed changes in activity of six rER-resident PPIases, cyclophilin B (encoded by the PPIB gene), FKBP13 (FKBP2), FKBP19 (FKBP11), FKBP22 (FKBP14), FKBP23 (FKBP7), and FKBP65 (FKBP10), due to posttranslational modifications of proline residues in the substrate.
Title: Ziploc-ing the structure 2.0: Endoplasmic reticulum-resident peptidyl prolyl isomerases show different activities toward hydroxyproline.

The data suggest that overexpressed CypB protects neuronal cells from MPP+-induced dopaminergic neuronal cell death
Title: Cyclophilin B protects SH-SY5Y human neuroblastoma cells against MPP(+)-induced neurotoxicity via JNK pathway.

Over-expression of CypB enhances HIV infection by increasing nuclear import of viral DNA.
Title: Cyclophilin B enhances HIV-1 infection.

This study enhances our knowledge about the mutational pattern of the LEPRE1, CRTAP, and PPIB genes. LEPRE1 should be the first gene analyzed in mutation detection studies in patients with recessive OI.
Title: Mutational characterization of the P3H1/CRTAP/CypB complex in recessive osteogenesis imperfecta.

Cyclophilin B has a high diagnostic value for stomach cancer and its downregulation can effectively inhibit the growth of stomach cancer cells. Thus, cyclophilin B may be a potential therapeutic target for stomach cancer treatment
Title: Expression and role of cyclophilin B in stomach cancer.

The following PPIB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPIB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu19649
NM_000942.4
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Homo sapiens peptidylprolyl isomerase B (PPIB), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu19649
Clone ID Related Accession (Same CDS sequence) NM_000942.4
Accession Version NM_000942.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 651bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-26
Organism Homo sapiens(Human)
Product peptidyl-prolyl cis-trans isomerase B precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BQ931949.1, BC008848.2 and BC001125.2. This sequence is a reference standard in the RefSeqGene project. On Mar 2, 2004 this sequence version replaced NM_000942.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU794651.1, SRR1163658.412242.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
ATGCTGCGCC TCTCCGAACG CAACATGAAG GTGCTCCTTG CCGCCGCCCT CATCGCGGGG 
TCCGTCTTCT TCCTGCTGCT GCCGGGACCT TCTGCGGCCG ATGAGAAGAA GAAGGGGCCC
AAAGTCACCG TCAAGGTGTA TTTTGACCTA CGAATTGGAG ATGAAGATGT AGGCCGGGTG
ATCTTTGGTC TCTTCGGAAA GACTGTTCCA AAAACAGTGG ATAATTTTGT GGCCTTAGCT
ACAGGAGAGA AAGGATTTGG CTACAAAAAC AGCAAATTCC ATCGTGTAAT CAAGGACTTC
ATGATCCAGG GCGGAGACTT CACCAGGGGA GATGGCACAG GAGGAAAGAG CATCTACGGT
GAGCGCTTCC CCGATGAGAA CTTCAAACTG AAGCACTACG GGCCTGGCTG GGTGAGCATG
GCCAACGCAG GCAAAGACAC CAACGGCTCC CAGTTCTTCA TCACGACAGT CAAGACAGCC
TGGCTAGATG GCAAGCATGT GGTGTTTGGC AAAGTTCTAG AGGGCATGGA GGTGGTGCGG
AAGGTGGAGA GCACCAAGAC AGACAGCCGG GATAAACCCC TGAAGGATGT GATCATCGCA
GACTGCGGCA AGATCGAGGT GGAGAAGCCC TTTGCCATCG CCAAGGAGTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000933.1
CDS170..820
Misc Feature(1)302..778
Misc Feature(2)order(449..454,467..469,620..622,626..628,650..652)
Misc Feature(3)773..775
Misc Feature(4)794..796
Misc Feature(5)794..796
Misc Feature(6)806..817
Exon (1)1..304
Exon (2)305..418
Exon (3)419..512
Exon (4)513..697
Exon (5)698..1028
Translation

Target ORF information:

RefSeq Version NM_000942.4
Organism Homo sapiens(Human)
Definition Homo sapiens peptidylprolyl isomerase B (PPIB), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000942.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGCTGCGCC TCTCCGAACG CAACATGAAG GTGCTCCTTG CCGCCGCCCT CATCGCGGGG 
TCCGTCTTCT TCCTGCTGCT GCCGGGACCT TCTGCGGCCG ATGAGAAGAA GAAGGGGCCC
AAAGTCACCG TCAAGGTGTA TTTTGACCTA CGAATTGGAG ATGAAGATGT AGGCCGGGTG
ATCTTTGGTC TCTTCGGAAA GACTGTTCCA AAAACAGTGG ATAATTTTGT GGCCTTAGCT
ACAGGAGAGA AAGGATTTGG CTACAAAAAC AGCAAATTCC ATCGTGTAAT CAAGGACTTC
ATGATCCAGG GCGGAGACTT CACCAGGGGA GATGGCACAG GAGGAAAGAG CATCTACGGT
GAGCGCTTCC CCGATGAGAA CTTCAAACTG AAGCACTACG GGCCTGGCTG GGTGAGCATG
GCCAACGCAG GCAAAGACAC CAACGGCTCC CAGTTCTTCA TCACGACAGT CAAGACAGCC
TGGCTAGATG GCAAGCATGT GGTGTTTGGC AAAGTTCTAG AGGGCATGGA GGTGGTGCGG
AAGGTGGAGA GCACCAAGAC AGACAGCCGG GATAAACCCC TGAAGGATGT GATCATCGCA
GACTGCGGCA AGATCGAGGT GGAGAAGCCC TTTGCCATCG CCAAGGAGTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Cyclophilin B enhances HIV-1 infection.
Virology489282-91(2016 Feb)
DeBoer J,Madson CJ,Belshan M


book

Mutational characterization of the P3H1/CRTAP/CypB complex in recessive osteogenesis imperfecta.
Genetics and molecular research : GMR14(4)15848-58(2015 Dec)
Barbirato C,Trancozo M,Almeida MG,Almeida LS,Santos TO,Duarte JC,Rebouças MR,Sipolatti V,Nunes VR,Paula F


book

Expression and role of cyclophilin B in stomach cancer.
Genetics and molecular research : GMR14(2)5346-54(2015 May)
Meng DQ,Li PL,Xie M


book

Depletion of cyclophilins B and C leads to dysregulation of endoplasmic reticulum redox homeostasis.
The Journal of biological chemistry289(33)23086-96(2014 Aug)
Stocki P,Chapman DC,Beach LA,Williams DB


book

Functional aspects of extracellular cyclophilins.
Biological chemistry395(7-8)721-35(2014 Jul)
Hoffmann H,Schiene-Fischer C


 
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