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PRKACA cDNA ORF clone, Homo sapiens(Human)

Gene Symbol PRKACA
Entrez Gene ID 5566
Full Name protein kinase cAMP-activated catalytic subunit alpha
Synonyms PKACA,PPNAD4
General protein information
Preferred Names

protein kinase cAMP-activated catalytic subunit alpha

Names

cAMP-dependent protein kinase catalytic subunit alpha
PKA C-alpha
protein kinase A catalytic subunit
protein kinase, cAMP-dependent, alpha catalytic subunit
protein kinase, cAMP-dependent, catalytic, alpha

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

19

19p13.12

Summary This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms. [provided by RefSeq, Jan 2015].
Disorder MIM:

601639


mRNA and Protein(s)

mRNA Protein Name
NM_207518.2 NP_997401.1 cAMP-dependent protein kinase catalytic subunit alpha isoform Calpha2
NM_001304349.1 NP_001291278.1 cAMP-dependent protein kinase catalytic subunit alpha isoform 3
NM_002730.3 NP_002721.1 cAMP-dependent protein kinase catalytic subunit alpha isoform Calpha1
XM_017026948.1 XP_016882437.1 cAMP-dependent protein kinase catalytic subunit alpha isoform X1



Mus musculus (house mouse) Prkaca NP_032880.1
Danio rerio (zebrafish) prkacab NP_001003470.1
Kluyveromyces lactis KLLA0D03190g XP_453207.1
Homo sapiens (human) PRKACA NP_002721.1
Bos taurus (cattle) PRKACA NP_777009.1
Schizosaccharomyces pombe (fission yeast) pka1 NP_595159.1
Xenopus tropicalis (tropical clawed frog) prkaca NP_001016403.1
Pan troglodytes (chimpanzee) PRKACA XP_001171437.1
Saccharomyces cerevisiae (baker's yeast) TPK2 NP_015121.1
Eremothecium gossypii AGOS_AFL090W NP_985458.1
Rattus norvegicus (Norway rat) Prkaca NP_001094392.1
Canis lupus familiaris (dog) PRKACA NP_001003032.1


Related articles in PubMed

G Protein Alpha S Subunit Promotes Cell Proliferation of Renal Cell Carcinoma with Involvement of Protein Kinase A Signaling.
Zhang B, Sun N, Mu X, Zhi L, Zhai L, Jiang Y, Fu Z, Yao Z
DNA and cell biology36(3)237-242(2017 Mar)

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Yachie N, Petsalaki E, Mellor JC, Weile J, Jacob Y, Verby M, Ozturk SB, Li S, Cote AG, Mosca R, Knapp JJ, Ko M, Yu A, Gebbia M, Sahni N, Yi S, Tyagi T, Sheykhkarimli D, Roth JF, Wong C, Musa L, Snider J, Liu YC, Yu H, Braun P, Stagljar I, Hao T, Calderwood MA, Pelletier L, Aloy P, Hill DE, Vidal M, Roth FP
Molecular systems biology12(4)863(2016 Apr)

Protein Kinase A Activation Promotes Cancer Cell Resistance to Glucose Starvation and Anoikis.
Palorini R, Votta G, Pirola Y, De Vitto H, De Palma S, Airoldi C, Vasso M, Ricciardiello F, Lombardi PP, Cirulli C, Rizzi R, Nicotra F, Hiller K, Gelfi C, Alberghina L, Chiaradonna F
PLoS genetics12(3)e1005931(2016 Mar)

Constitutive activation of PKA catalytic subunit in adrenal Cushing's syndrome.
Beuschlein F, Fassnacht M, Assié G, Calebiro D, Stratakis CA, Osswald A, Ronchi CL, Wieland T, Sbiera S, Faucz FR, Schaak K, Schmittfull A, Schwarzmayr T, Barreau O, Vezzosi D, Rizk-Rabin M, Zabel U, Szarek E, Salpea P, Forlino A, Vetro A, Zuffardi O, Kisker C, Diener S, Meitinger T, Lohse MJ, Reincke M, Bertherat J, Strom TM, Allolio B
The New England journal of medicine370(11)1019-28(2014 Mar)

The unique catalytic subunit of sperm cAMP-dependent protein kinase is the product of an alternative Calpha mRNA expressed specifically in spermatogenic cells.
Agustin JT, Wilkerson CG, Witman GB
Molecular biology of the cell11(9)3031-44(2000 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The inhibitory effects of BMP4 on PDGF-induced cell proliferation, collagen synthesis, and calpain-2 activation are impaired in pulmonary artery smooth muscle cells from pulmonary arterial hypertension patient.
Title: BMP4 inhibits PDGF-induced proliferation and collagen synthesis via PKA-mediated inhibition of calpain-2 in pulmonary artery smooth muscle cells.

testosterone rapidly increased whole-cell HCAEC SKCa and BKCa currents via a surface androgen receptor, Gi/o protein, and protein kinase A
Title: Testosterone rapidly increases Ca

Studies indicate the 3'-5'-Cyclic adenosine monophosphate/protein kinase A (cAMP/PKA) signalling pathway at distinct mitochondria subdomains represented by the outer and inner mitochondrial membranes.
Title: Components of the mitochondrial cAMP signalosome.

Studies indicate a functional interaction between leucine-rich repeat kinase 2 (LRRK2) and protein kinase A (PKA) cross-talk in neuron and microglia.
Title: Cross-talk between LRRK2 and PKA: implication for Parkinson's disease?

high-resolution models for PKA(WT) and PKA(L)(205R) substrate specificity.
Title: Cushing's syndrome mutant PKA

The following PRKACA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRKACA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu18669
NM_002730.3
Latest version!
Homo sapiens protein kinase cAMP-activated catalytic subunit alpha (PRKACA), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu17314 NM_207518.2
Latest version!
Homo sapiens protein kinase cAMP-activated catalytic subunit alpha (PRKACA), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $202.30
$289.00
OHu51697 NM_001304349.1
Latest version!
Homo sapiens protein kinase cAMP-activated catalytic subunit alpha (PRKACA), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu88549 XM_017026948.1
Latest version!
Homo sapiens protein kinase cAMP-activated catalytic subunit alpha (PRKACA), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $139.30
$199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu18669
Clone ID Related Accession (Same CDS sequence) NM_002730.3
Accession Version NM_002730.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1056bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-26
Organism Homo sapiens(Human)
Product cAMP-dependent protein kinase catalytic subunit alpha isoform Calpha1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU543573.1 and BC039846.1. This sequence is a reference standard in the RefSeqGene project. On Apr 30, 2004 this sequence version replaced NM_002730.2. Transcript Variant: This variant (1) encodes Calpha1 (PMID:21812984). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC108259.1, SRR1163657.242192.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

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961
1021
ATGGGCAACG CCGCCGCCGC CAAGAAGGGC AGCGAGCAGG AGAGCGTGAA AGAATTCTTA 
GCCAAAGCCA AAGAAGATTT TCTTAAAAAA TGGGAAAGTC CCGCTCAGAA CACAGCCCAC
TTGGATCAGT TTGAACGAAT CAAGACCCTC GGCACGGGCT CCTTCGGGCG GGTGATGCTG
GTGAAACACA AGGAGACCGG GAACCACTAT GCCATGAAGA TCCTCGACAA ACAGAAGGTG
GTGAAACTGA AACAGATCGA ACACACCCTG AATGAAAAGC GCATCCTGCA AGCTGTCAAC
TTTCCGTTCC TCGTCAAACT CGAGTTCTCC TTCAAGGACA ACTCAAACTT ATACATGGTC
ATGGAGTACG TGCCCGGCGG GGAGATGTTC TCACACCTAC GGCGGATCGG AAGGTTCAGT
GAGCCCCATG CCCGTTTCTA CGCGGCCCAG ATCGTCCTGA CCTTTGAGTA TCTGCACTCG
CTGGATCTCA TCTACAGGGA CCTGAAGCCG GAGAATCTGC TCATTGACCA GCAGGGCTAC
ATTCAGGTGA CAGACTTCGG TTTCGCCAAG CGCGTGAAGG GCCGCACTTG GACCTTGTGC
GGCACCCCTG AGTACCTGGC CCCTGAGATT ATCCTGAGCA AAGGCTACAA CAAGGCCGTG
GACTGGTGGG CCCTGGGGGT TCTTATCTAT GAAATGGCCG CTGGCTACCC GCCCTTCTTC
GCAGACCAGC CCATCCAGAT CTATGAGAAG ATCGTCTCTG GGAAGGTGCG CTTCCCTTCC
CACTTCAGCT CTGACTTGAA GGACCTGCTG CGGAACCTCC TGCAGGTAGA TCTCACCAAG
CGCTTTGGGA ACCTCAAGAA TGGGGTCAAC GATATCAAGA ACCACAAGTG GTTTGCCACA
ACTGACTGGA TTGCCATCTA CCAGAGGAAG GTGGAAGCTC CCTTCATACC AAAGTTTAAA
GGCCCTGGGG ATACGAGTAA CTTTGACGAC TATGAGGAAG AAGAAATCCG GGTCTCCATC
AATGAGAAGT GTGGCAAGGA GTTTTCTGAG TTTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002721.1
CDS201..1256
Misc Feature(1)207..209
Misc Feature(2)231..233
Misc Feature(3)231..233
Misc Feature(4)324..1193
Misc Feature(5)330..1094
Misc Feature(6)345..347
Misc Feature(7)order(348..368,372..374,411..413,417..419,513..515,561..572,582..584,699..701,705..707,711..716,720..722,750..755,1182..1184)
Misc Feature(8)order(351..368,372..374,411..413,417..419,447..449,513..515,561..566,570..572,582..584,588..590,600..602,699..701,705..716,720..722,750..755,762..764,795..797,801..812,891..893,903..911,918..926,930..932,939..941,1182..1184,1191..1193)
Misc Feature(9)order(354..356,360..365,447..455,582..584,588..590,600..602,699..701,705..713,762..764,795..812,891..893,903..911,918..926,930..932,939..944,1191..1193)
Misc Feature(10)order(360..362,450..455,459..464,582..584,588..590,600..602,705..713,762..764,783..785,789..806,813..815,834..842,891..893,930..935,942..947,954..956,1191..1193)
Misc Feature(11)618..620
Misc Feature(12)618..620
Misc Feature(13)750..812
Misc Feature(14)786..788
Misc Feature(15)786..788
Misc Feature(16)792..794
Misc Feature(17)792..794
Misc Feature(18)804..806
Misc Feature(19)804..806
Misc Feature(20)1191..1193
Misc Feature(21)1215..1217
Misc Feature(22)1215..1217
Exon (1)1..246
Exon (2)247..308
Exon (3)309..437
Exon (4)438..536
Exon (5)537..619
Exon (6)620..746
Exon (7)747..842
Exon (8)843..965
Exon (9)966..1130
Exon (10)1131..2680
Translation

Target ORF information:

RefSeq Version NM_002730.3
Organism Homo sapiens(Human)
Definition Homo sapiens protein kinase cAMP-activated catalytic subunit alpha (PRKACA), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002730.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGGCAACG CCGCCGCCGC CAAGAAGGGC AGCGAGCAGG AGAGCGTGAA AGAATTCTTA 
GCCAAAGCCA AAGAAGATTT TCTTAAAAAA TGGGAAAGTC CCGCTCAGAA CACAGCCCAC
TTGGATCAGT TTGAACGAAT CAAGACCCTC GGCACGGGCT CCTTCGGGCG GGTGATGCTG
GTGAAACACA AGGAGACCGG GAACCACTAT GCCATGAAGA TCCTCGACAA ACAGAAGGTG
GTGAAACTGA AACAGATCGA ACACACCCTG AATGAAAAGC GCATCCTGCA AGCTGTCAAC
TTTCCGTTCC TCGTCAAACT CGAGTTCTCC TTCAAGGACA ACTCAAACTT ATACATGGTC
ATGGAGTACG TGCCCGGCGG GGAGATGTTC TCACACCTAC GGCGGATCGG AAGGTTCAGT
GAGCCCCATG CCCGTTTCTA CGCGGCCCAG ATCGTCCTGA CCTTTGAGTA TCTGCACTCG
CTGGATCTCA TCTACAGGGA CCTGAAGCCG GAGAATCTGC TCATTGACCA GCAGGGCTAC
ATTCAGGTGA CAGACTTCGG TTTCGCCAAG CGCGTGAAGG GCCGCACTTG GACCTTGTGC
GGCACCCCTG AGTACCTGGC CCCTGAGATT ATCCTGAGCA AAGGCTACAA CAAGGCCGTG
GACTGGTGGG CCCTGGGGGT TCTTATCTAT GAAATGGCCG CTGGCTACCC GCCCTTCTTC
GCAGACCAGC CCATCCAGAT CTATGAGAAG ATCGTCTCTG GGAAGGTGCG CTTCCCTTCC
CACTTCAGCT CTGACTTGAA GGACCTGCTG CGGAACCTCC TGCAGGTAGA TCTCACCAAG
CGCTTTGGGA ACCTCAAGAA TGGGGTCAAC GATATCAAGA ACCACAAGTG GTTTGCCACA
ACTGACTGGA TTGCCATCTA CCAGAGGAAG GTGGAAGCTC CCTTCATACC AAAGTTTAAA
GGCCCTGGGG ATACGAGTAA CTTTGACGAC TATGAGGAAG AAGAAATCCG GGTCTCCATC
AATGAGAAGT GTGGCAAGGA GTTTTCTGAG TTTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu17314
Clone ID Related Accession (Same CDS sequence) NM_207518.2
Accession Version NM_207518.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1032bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product cAMP-dependent protein kinase catalytic subunit alpha isoform Calpha2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK292284.1, BC108259.1 and BC039846.1. On Jan 17, 2015 this sequence version replaced NM_207518.1. Transcript Variant: This variant (2) contains an alternate 5' terminal exon and initiates translation at an alternate start codon, compared to variant 1. It encodes Calpha2, PMID:21812984 (also known as C alpha-s, PMID: 10906071) which has a shorter and distinct N-terminus, compared to Calpha1. This variant is specifically expressed in spermatogenic cells and may be essential for male fertility. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292284.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

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ATGGCTTCCA ACTCCAGCGA TGTGAAAGAA TTCTTAGCCA AAGCCAAAGA AGATTTTCTT 
AAAAAATGGG AAAGTCCCGC TCAGAACACA GCCCACTTGG ATCAGTTTGA ACGAATCAAG
ACCCTCGGCA CGGGCTCCTT CGGGCGGGTG ATGCTGGTGA AACACAAGGA GACCGGGAAC
CACTATGCCA TGAAGATCCT CGACAAACAG AAGGTGGTGA AACTGAAACA GATCGAACAC
ACCCTGAATG AAAAGCGCAT CCTGCAAGCT GTCAACTTTC CGTTCCTCGT CAAACTCGAG
TTCTCCTTCA AGGACAACTC AAACTTATAC ATGGTCATGG AGTACGTGCC CGGCGGGGAG
ATGTTCTCAC ACCTACGGCG GATCGGAAGG TTCAGTGAGC CCCATGCCCG TTTCTACGCG
GCCCAGATCG TCCTGACCTT TGAGTATCTG CACTCGCTGG ATCTCATCTA CAGGGACCTG
AAGCCGGAGA ATCTGCTCAT TGACCAGCAG GGCTACATTC AGGTGACAGA CTTCGGTTTC
GCCAAGCGCG TGAAGGGCCG CACTTGGACC TTGTGCGGCA CCCCTGAGTA CCTGGCCCCT
GAGATTATCC TGAGCAAAGG CTACAACAAG GCCGTGGACT GGTGGGCCCT GGGGGTTCTT
ATCTATGAAA TGGCCGCTGG CTACCCGCCC TTCTTCGCAG ACCAGCCCAT CCAGATCTAT
GAGAAGATCG TCTCTGGGAA GGTGCGCTTC CCTTCCCACT TCAGCTCTGA CTTGAAGGAC
CTGCTGCGGA ACCTCCTGCA GGTAGATCTC ACCAAGCGCT TTGGGAACCT CAAGAATGGG
GTCAACGATA TCAAGAACCA CAAGTGGTTT GCCACAACTG ACTGGATTGC CATCTACCAG
AGGAAGGTGG AAGCTCCCTT CATACCAAAG TTTAAAGGCC CTGGGGATAC GAGTAACTTT
GACGACTATG AGGAAGAAGA AATCCGGGTC TCCATCAATG AGAAGTGTGG CAAGGAGTTT
TCTGAGTTTT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_997401.1
CDS34..1065
Misc Feature(1)28..30
Misc Feature(2)133..1002
Misc Feature(3)139..903
Misc Feature(4)order(157..177,181..183,220..222,226..228,322..324,370..381,391..393,508..510,514..516,520..525,529..531,559..564,991..993)
Misc Feature(5)order(160..177,181..183,220..222,226..228,256..258,322..324,370..375,379..381,391..393,397..399,409..411,508..510,514..525,529..531,559..564,571..573,604..606,610..621,700..702,712..720,727..735,739..741,748..750,991..993,1000..1002)
Misc Feature(6)order(163..165,169..174,256..264,391..393,397..399,409..411,508..510,514..522,571..573,604..621,700..702,712..720,727..735,739..741,748..753,1000..1002)
Misc Feature(7)order(169..171,259..264,268..273,391..393,397..399,409..411,514..522,571..573,592..594,598..615,622..624,643..651,700..702,739..744,751..756,763..765,1000..1002)
Misc Feature(8)427..429
Misc Feature(9)559..621
Misc Feature(10)595..597
Misc Feature(11)601..603
Misc Feature(12)613..615
Misc Feature(13)1024..1026
Exon (1)1..55
Exon (2)56..117
Exon (3)118..246
Exon (4)247..345
Exon (5)346..428
Exon (6)429..555
Exon (7)556..651
Exon (8)652..774
Exon (9)775..939
Exon (10)940..2489
Translation

Target ORF information:

RefSeq Version NM_207518.2
Organism Homo sapiens(Human)
Definition Homo sapiens protein kinase cAMP-activated catalytic subunit alpha (PRKACA), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_207518.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCTTCCA ACTCCAGCGA TGTGAAAGAA TTCTTAGCCA AAGCCAAAGA AGATTTTCTT 
AAAAAATGGG AAAGTCCCGC TCAGAACACA GCCCACTTGG ATCAGTTTGA ACGAATCAAG
ACCCTCGGCA CGGGCTCCTT CGGGCGGGTG ATGCTGGTGA AACACAAGGA GACCGGGAAC
CACTATGCCA TGAAGATCCT CGACAAACAG AAGGTGGTGA AACTGAAACA GATCGAACAC
ACCCTGAATG AAAAGCGCAT CCTGCAAGCT GTCAACTTTC CGTTCCTCGT CAAACTCGAG
TTCTCCTTCA AGGACAACTC AAACTTATAC ATGGTCATGG AGTACGTGCC CGGCGGGGAG
ATGTTCTCAC ACCTACGGCG GATCGGAAGG TTCAGTGAGC CCCATGCCCG TTTCTACGCG
GCCCAGATCG TCCTGACCTT TGAGTATCTG CACTCGCTGG ATCTCATCTA CAGGGACCTG
AAGCCGGAGA ATCTGCTCAT TGACCAGCAG GGCTACATTC AGGTGACAGA CTTCGGTTTC
GCCAAGCGCG TGAAGGGCCG CACTTGGACC TTGTGCGGCA CCCCTGAGTA CCTGGCCCCT
GAGATTATCC TGAGCAAAGG CTACAACAAG GCCGTGGACT GGTGGGCCCT GGGGGTTCTT
ATCTATGAAA TGGCCGCTGG CTACCCGCCC TTCTTCGCAG ACCAGCCCAT CCAGATCTAT
GAGAAGATCG TCTCTGGGAA GGTGCGCTTC CCTTCCCACT TCAGCTCTGA CTTGAAGGAC
CTGCTGCGGA ACCTCCTGCA GGTAGATCTC ACCAAGCGCT TTGGGAACCT CAAGAATGGG
GTCAACGATA TCAAGAACCA CAAGTGGTTT GCCACAACTG ACTGGATTGC CATCTACCAG
AGGAAGGTGG AAGCTCCCTT CATACCAAAG TTTAAAGGCC CTGGGGATAC GAGTAACTTT
GACGACTATG AGGAAGAAGA AATCCGGGTC TCCATCAATG AGAAGTGTGG CAAGGAGTTT
TCTGAGTTTT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu51697
Clone ID Related Accession (Same CDS sequence) NM_001304349.1
Accession Version NM_001304349.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1284bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product cAMP-dependent protein kinase catalytic subunit alpha isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK131561.1, BC108259.1 and BC039846.1. On Jan 17, 2015 this sequence version replaced XM_005259984.1. Transcript Variant: This variant (3) contains an alternate 5' terminal exon and initiates translation at an alternate start codon, compared to variant 1. It encodes isoform 3 which has a longer and distinct N-terminus, compared to Calpha1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK131561.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2159607 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
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361
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481
541
601
661
721
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841
901
961
1021
1081
1141
1201
1261
ATGTTCACCT TGGGATACCA TGGGGCCGAG GAGTGGGCAT CGGGCGAGAC TTCCCTTGAA 
CCAGCTCTGG AGCAATGCCC TTGCGATTGC CACGGAGAGG AACAGGGCCA GCTGGGGGCC
AAGGTGACAG CTGAGCCCCC CAAGGGCACC TGCTACCCGA GCCCCCGAGG CCTCCATCCC
AGGCCCGGCC TCCCCAGAGA AGGGGCCAGA GGAACAGCCA AATCCCAAGG CTATAAATAC
CTCGGCTGGC GTCGGCCCTG GCCATGGAAT GTCGTGAAAG AATTCTTAGC CAAAGCCAAA
GAAGATTTTC TTAAAAAATG GGAAAGTCCC GCTCAGAACA CAGCCCACTT GGATCAGTTT
GAACGAATCA AGACCCTCGG CACGGGCTCC TTCGGGCGGG TGATGCTGGT GAAACACAAG
GAGACCGGGA ACCACTATGC CATGAAGATC CTCGACAAAC AGAAGGTGGT GAAACTGAAA
CAGATCGAAC ACACCCTGAA TGAAAAGCGC ATCCTGCAAG CTGTCAACTT TCCGTTCCTC
GTCAAACTCG AGTTCTCCTT CAAGGACAAC TCAAACTTAT ACATGGTCAT GGAGTACGTG
CCCGGCGGGG AGATGTTCTC ACACCTACGG CGGATCGGAA GGTTCAGTGA GCCCCATGCC
CGTTTCTACG CGGCCCAGAT CGTCCTGACC TTTGAGTATC TGCACTCGCT GGATCTCATC
TACAGGGACC TGAAGCCGGA GAATCTGCTC ATTGACCAGC AGGGCTACAT TCAGGTGACA
GACTTCGGTT TCGCCAAGCG CGTGAAGGGC CGCACTTGGA CCTTGTGCGG CACCCCTGAG
TACCTGGCCC CTGAGATTAT CCTGAGCAAA GGCTACAACA AGGCCGTGGA CTGGTGGGCC
CTGGGGGTTC TTATCTATGA AATGGCCGCT GGCTACCCGC CCTTCTTCGC AGACCAGCCC
ATCCAGATCT ATGAGAAGAT CGTCTCTGGG AAGGTGCGCT TCCCTTCCCA CTTCAGCTCT
GACTTGAAGG ACCTGCTGCG GAACCTCCTG CAGGTAGATC TCACCAAGCG CTTTGGGAAC
CTCAAGAATG GGGTCAACGA TATCAAGAAC CACAAGTGGT TTGCCACAAC TGACTGGATT
GCCATCTACC AGAGGAAGGT GGAAGCTCCC TTCATACCAA AGTTTAAAGG CCCTGGGGAT
ACGAGTAACT TTGACGACTA TGAGGAAGAA GAAATCCGGG TCTCCATCAA TGAGAAGTGT
GGCAAGGAGT TTTCTGAGTT TTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001291278.1
CDS407..1690
Misc Feature(1)341..343
Misc Feature(2)758..1627
Misc Feature(3)764..1528
Misc Feature(4)order(782..802,806..808,845..847,851..853,947..949,995..1006,1016..1018,1133..1135,1139..1141,1145..1150,1154..1156,1184..1189,1616..1618)
Misc Feature(5)order(785..802,806..808,845..847,851..853,881..883,947..949,995..1000,1004..1006,1016..1018,1022..1024,1034..1036,1133..1135,1139..1150,1154..1156,1184..1189,1196..1198,1229..1231,1235..1246,1325..1327,1337..1345,1352..1360,1364..1366,1373..1375,1616..1618,1625..1627)
Misc Feature(6)order(788..790,794..799,881..889,1016..1018,1022..1024,1034..1036,1133..1135,1139..1147,1196..1198,1229..1246,1325..1327,1337..1345,1352..1360,1364..1366,1373..1378,1625..1627)
Misc Feature(7)order(794..796,884..889,893..898,1016..1018,1022..1024,1034..1036,1139..1147,1196..1198,1217..1219,1223..1240,1247..1249,1268..1276,1325..1327,1364..1369,1376..1381,1388..1390,1625..1627)
Misc Feature(8)1184..1246
Exon (1)1..680
Exon (2)681..742
Exon (3)743..871
Exon (4)872..970
Exon (5)971..1053
Exon (6)1054..1180
Exon (7)1181..1276
Exon (8)1277..1399
Exon (9)1400..1564
Exon (10)1565..3114
Translation

Target ORF information:

RefSeq Version NM_001304349.1
Organism Homo sapiens(Human)
Definition Homo sapiens protein kinase cAMP-activated catalytic subunit alpha (PRKACA), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001304349.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGTTCACCT TGGGATACCA TGGGGCCGAG GAGTGGGCAT CGGGCGAGAC TTCCCTTGAA 
CCAGCTCTGG AGCAATGCCC TTGCGATTGC CACGGAGAGG AACAGGGCCA GCTGGGGGCC
AAGGTGACAG CTGAGCCCCC CAAGGGCACC TGCTACCCGA GCCCCCGAGG CCTCCATCCC
AGGCCCGGCC TCCCCAGAGA AGGGGCCAGA GGAACAGCCA AATCCCAAGG CTATAAATAC
CTCGGCTGGC GTCGGCCCTG GCCATGGAAT GTCGTGAAAG AATTCTTAGC CAAAGCCAAA
GAAGATTTTC TTAAAAAATG GGAAAGTCCC GCTCAGAACA CAGCCCACTT GGATCAGTTT
GAACGAATCA AGACCCTCGG CACGGGCTCC TTCGGGCGGG TGATGCTGGT GAAACACAAG
GAGACCGGGA ACCACTATGC CATGAAGATC CTCGACAAAC AGAAGGTGGT GAAACTGAAA
CAGATCGAAC ACACCCTGAA TGAAAAGCGC ATCCTGCAAG CTGTCAACTT TCCGTTCCTC
GTCAAACTCG AGTTCTCCTT CAAGGACAAC TCAAACTTAT ACATGGTCAT GGAGTACGTG
CCCGGCGGGG AGATGTTCTC ACACCTACGG CGGATCGGAA GGTTCAGTGA GCCCCATGCC
CGTTTCTACG CGGCCCAGAT CGTCCTGACC TTTGAGTATC TGCACTCGCT GGATCTCATC
TACAGGGACC TGAAGCCGGA GAATCTGCTC ATTGACCAGC AGGGCTACAT TCAGGTGACA
GACTTCGGTT TCGCCAAGCG CGTGAAGGGC CGCACTTGGA CCTTGTGCGG CACCCCTGAG
TACCTGGCCC CTGAGATTAT CCTGAGCAAA GGCTACAACA AGGCCGTGGA CTGGTGGGCC
CTGGGGGTTC TTATCTATGA AATGGCCGCT GGCTACCCGC CCTTCTTCGC AGACCAGCCC
ATCCAGATCT ATGAGAAGAT CGTCTCTGGG AAGGTGCGCT TCCCTTCCCA CTTCAGCTCT
GACTTGAAGG ACCTGCTGCG GAACCTCCTG CAGGTAGATC TCACCAAGCG CTTTGGGAAC
CTCAAGAATG GGGTCAACGA TATCAAGAAC CACAAGTGGT TTGCCACAAC TGACTGGATT
GCCATCTACC AGAGGAAGGT GGAAGCTCCC TTCATACCAA AGTTTAAAGG CCCTGGGGAT
ACGAGTAACT TTGACGACTA TGAGGAAGAA GAAATCCGGG TCTCCATCAA TGAGAAGTGT
GGCAAGGAGT TTTCTGAGTT TTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu88549
Clone ID Related Accession (Same CDS sequence) XM_017026948.1
Accession Version XM_017026948.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 891bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product cAMP-dependent protein kinase catalytic subunit alpha isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGGCAACG CCGCCGCCGC CAAGAAGGGC AGCGAGCAGG AGAGCGTGAA AGAATTCTTA 
GCCAAAGCCA AAGAAGATTT TCTTAAAAAA TGGGAAAGTC CCGCTCAGAA CACAGCCCAC
TTGGATCAGT TTGAACGAAT CAAGACCCTC GGCACGGGCT CCTTCGGGCG GGTGATGCTG
GTGAAACACA AGGAGACCGG GAACCACTAT GCCATGAAGA TCCTCGACAA ACAGAAGGTG
GTGAAACTGA AACAGATCGA ACACACCCTG AATGAAAAGC GCATCCTGCA AGCTGTCAAC
TTTCCGTTCC TCGTCAAACT CGAGTTCTCC TTCAAGGACA ACTCAAACTT ATACATGGTC
ATGGAGTACG TGCCCGGCGG GGAGATGTTC TCACACCTAC GGCGGATCGG AAGGTTCAGT
GAGCCCCATG CCCGTTTCTA CGCGGCCCAG ATCGTCCTGA CCTTTGAGTA TCTGCACTCG
CTGGATCTCA TCTACAGGGA CCTGAAGCCG GAGAATCTGC TCATTGACCA GCAGGGCTAC
ATTCAGGTGA CAGACTTCGG TTTCGCCAAG CGCGTGAAGG GCCGCACTTG GACCTTGTGC
GGCACCCCTG AGTACCTGGC CCCTGAGATT ATCCTGAGCA AAGGCTACAA CAAGGCCGTG
GACTGGTGGG CCCTGGGGGT TCTTATCTAT GAAATGGCCG CTGGCTACCC GCCCTTCTTC
GCAGACCAGC CCATCCAGAT CTATGAGAAG ATCGTCTCTG GGAAGGTGGA AGCTCCCTTC
ATACCAAAGT TTAAAGGCCC TGGGGATACG AGTAACTTTG ACGACTATGA GGAAGAAGAA
ATCCGGGTCT CCATCAATGA GAAGTGTGGC AAGGAGTTTT CTGAGTTTTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016882437.1
CDS57..947
Translation

Target ORF information:

RefSeq Version XM_017026948.1
Organism Homo sapiens(Human)
Definition Homo sapiens protein kinase cAMP-activated catalytic subunit alpha (PRKACA), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017026948.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGGCAACG CCGCCGCCGC CAAGAAGGGC AGCGAGCAGG AGAGCGTGAA AGAATTCTTA 
GCCAAAGCCA AAGAAGATTT TCTTAAAAAA TGGGAAAGTC CCGCTCAGAA CACAGCCCAC
TTGGATCAGT TTGAACGAAT CAAGACCCTC GGCACGGGCT CCTTCGGGCG GGTGATGCTG
GTGAAACACA AGGAGACCGG GAACCACTAT GCCATGAAGA TCCTCGACAA ACAGAAGGTG
GTGAAACTGA AACAGATCGA ACACACCCTG AATGAAAAGC GCATCCTGCA AGCTGTCAAC
TTTCCGTTCC TCGTCAAACT CGAGTTCTCC TTCAAGGACA ACTCAAACTT ATACATGGTC
ATGGAGTACG TGCCCGGCGG GGAGATGTTC TCACACCTAC GGCGGATCGG AAGGTTCAGT
GAGCCCCATG CCCGTTTCTA CGCGGCCCAG ATCGTCCTGA CCTTTGAGTA TCTGCACTCG
CTGGATCTCA TCTACAGGGA CCTGAAGCCG GAGAATCTGC TCATTGACCA GCAGGGCTAC
ATTCAGGTGA CAGACTTCGG TTTCGCCAAG CGCGTGAAGG GCCGCACTTG GACCTTGTGC
GGCACCCCTG AGTACCTGGC CCCTGAGATT ATCCTGAGCA AAGGCTACAA CAAGGCCGTG
GACTGGTGGG CCCTGGGGGT TCTTATCTAT GAAATGGCCG CTGGCTACCC GCCCTTCTTC
GCAGACCAGC CCATCCAGAT CTATGAGAAG ATCGTCTCTG GGAAGGTGGA AGCTCCCTTC
ATACCAAAGT TTAAAGGCCC TGGGGATACG AGTAACTTTG ACGACTATGA GGAAGAAGAA
ATCCGGGTCT CCATCAATGA GAAGTGTGGC AAGGAGTTTT CTGAGTTTTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

G Protein Alpha S Subunit Promotes Cell Proliferation of Renal Cell Carcinoma with Involvement of Protein Kinase A Signaling.
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