PRKACA cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRKACA
Entrez Gene ID	5566
Full Name	protein kinase cAMP-activated catalytic subunit alpha
Synonyms	PKACA,PPNAD4
General protein information	Preferred Names protein kinase cAMP-activated catalytic subunit alpha Names cAMP-dependent protein kinase catalytic subunit alpha PKA C-alpha protein kinase A catalytic subunit protein kinase, cAMP-dependent, alpha catalytic subunit protein kinase, cAMP-dependent, catalytic, alpha
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.12
Summary	This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms. [provided by RefSeq, Jan 2015].
Disorder MIM:	601639
Disorder Html:	Cushing syndrome, ACTH-independent adrenal, somatic, 615830 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_207518.2	NP_997401.1	cAMP-dependent protein kinase catalytic subunit alpha isoform Calpha2
NM_002730.3	NP_002721.1	cAMP-dependent protein kinase catalytic subunit alpha isoform Calpha1
NM_001304349.1	NP_001291278.1	cAMP-dependent protein kinase catalytic subunit alpha isoform 3
XM_017026948.1	XP_016882437.1	cAMP-dependent protein kinase catalytic subunit alpha isoform X1
NM_002730.4	NP_002721.1	cAMP-dependent protein kinase catalytic subunit alpha isoform Calpha1
NM_207518.3	NP_997401.1	cAMP-dependent protein kinase catalytic subunit alpha isoform Calpha2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Prkaca	NP_032880.1
Danio rerio (zebrafish)	prkacab	NP_001003470.1
Kluyveromyces lactis	KLLA0D03190g	XP_453207.1
Homo sapiens (human)	PRKACA	NP_002721.1
Bos taurus (cattle)	PRKACA	NP_777009.1
Schizosaccharomyces pombe (fission yeast)	pka1	NP_595159.1
Xenopus tropicalis (tropical clawed frog)	prkaca	NP_001016403.1
Pan troglodytes (chimpanzee)	PRKACA	XP_001171437.1
Saccharomyces cerevisiae (baker's yeast)	TPK2	NP_015121.1
Eremothecium gossypii	AGOS_AFL090W	NP_985458.1
Rattus norvegicus (Norway rat)	Prkaca	NP_001094392.1
Canis lupus familiaris (dog)	PRKACA	NP_001003032.1

Gene Ontology
Bibliography

Related articles in PubMed

Constitutive activation of PKA catalytic subunit in adrenal Cushing's syndrome.
Beuschlein F, Fassnacht M, Assi? G, Calebiro D, Stratakis CA, Osswald A, Ronchi CL, Wieland T, Sbiera S, Faucz FR, Schaak K, Schmittfull A, Schwarzmayr T, Barreau O, Vezzosi D, Rizk-Rabin M, Zabel U, Szarek E, Salpea P, Forlino A, Vetro A, Zuffardi O, Kisker C, Diener S, Meitinger T, Lohse MJ, Reincke M, Bertherat J, Strom TM, Allolio B
The New England journal of medicine370(11)1019-28(2014 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we propose that the PKA-Smurf1-PIPKIgamma pathway has an important role in pulmonary tumorigenesis and imposes substantial clinical impact on development of novel diagnostic markers and therapeutic targets for lung cancer treatment.
Title: Smurf1 regulates lung cancer cell growth and migration through interaction with and ubiquitination of PIPKI?.

The mutated PRKACA proteins lost their ability to bind to PRKAR1A, and thereby lead to constitutive activation of the PKA pathway. Together with previous reports of PRKAR1A mutations in syndromic cardiac myxoma, our study demonstrates the importance of the PKA pathway in the tumourigenesis of cardiac myxoma.
Title: Microinsertions in PRKACA cause activation of the protein kinase A pathway in cardiac myxoma.

The inhibitory effects of BMP4 on PDGF-induced cell proliferation, collagen synthesis, and calpain-2 activation are impaired in pulmonary artery smooth muscle cells from pulmonary arterial hypertension patient.
Title: BMP4 inhibits PDGF-induced proliferation and collagen synthesis via PKA-mediated inhibition of calpain-2 in pulmonary artery smooth muscle cells.

Dual co-expression of human fetal Tau with PKA in Escherichia coli results in multisite Tau phosphorylation including also naturally occurring sites which were not previously considered in the context of 14-3-3 binding. Tau protein co-expressed with PKA displays tight functional interaction with 14-3-3 isoforms of a different type.
Title: Bacterial co-expression of human Tau protein with protein kinase A and 14-3-3 for studies of 14-3-3/phospho-Tau interaction.

cAMP/PKA signaling attenuated respiratory syncytial virus-induced disruption of structure and functions of the model airway epithelial barrier by mechanisms involving the stabilization of epithelial junctions and inhibition of viral biogenesis
Title: cAMP-dependent activation of protein kinase A attenuates respiratory syncytial virus-induced human airway epithelial barrier disruption.

The following PRKACA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRKACA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18669	NM_002730.4 Latest version!	Homo sapiens protein kinase cAMP-activated catalytic subunit alpha (PRKACA), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu51697	NM_001304349.1 Latest version!	Homo sapiens protein kinase cAMP-activated catalytic subunit alpha (PRKACA), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu17314	NM_207518.3 Latest version!	Homo sapiens protein kinase cAMP-activated catalytic subunit alpha (PRKACA), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$202.30 $289.00
OHu88549	XM_017026948.1 Latest version!	Homo sapiens protein kinase cAMP-activated catalytic subunit alpha (PRKACA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OHu18669	NM_002730.3	Homo sapiens protein kinase cAMP-activated catalytic subunit alpha (PRKACA), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu17314	NM_207518.2	Homo sapiens protein kinase cAMP-activated catalytic subunit alpha (PRKACA), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$202.30 $289.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRKACA ( NM_002730.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002721.1 Homo sapiens protein kinase cAMP-activated catalytic subunit alpha (PRKACA), transcript variant 1, mRNA.

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CloneID	OHu18669
Clone ID Related Accession (Same CDS sequence)	NM_002730.3 , NM_002730.4
Accession Version	NM_002730.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	cAMP-dependent protein kinase catalytic subunit alpha isoform Calpha1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU543573.1 and BC039846.1. This sequence is a reference standard in the RefSeqGene project. On Apr 30, 2004 this sequence version replaced NM_002730.2. Transcript Variant: This variant (1) encodes Calpha1 (PMID:21812984). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC108259.1, SRR1163657.242192.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCAACG CCGCCGCCGC CAAGAAGGGC AGCGAGCAGG AGAGCGTGAA AGAATTCTTA 
GCCAAAGCCA AAGAAGATTT TCTTAAAAAA TGGGAAAGTC CCGCTCAGAA CACAGCCCAC 
TTGGATCAGT TTGAACGAAT CAAGACCCTC GGCACGGGCT CCTTCGGGCG GGTGATGCTG 
GTGAAACACA AGGAGACCGG GAACCACTAT GCCATGAAGA TCCTCGACAA ACAGAAGGTG 
GTGAAACTGA AACAGATCGA ACACACCCTG AATGAAAAGC GCATCCTGCA AGCTGTCAAC 
TTTCCGTTCC TCGTCAAACT CGAGTTCTCC TTCAAGGACA ACTCAAACTT ATACATGGTC 
ATGGAGTACG TGCCCGGCGG GGAGATGTTC TCACACCTAC GGCGGATCGG AAGGTTCAGT 
GAGCCCCATG CCCGTTTCTA CGCGGCCCAG ATCGTCCTGA CCTTTGAGTA TCTGCACTCG 
CTGGATCTCA TCTACAGGGA CCTGAAGCCG GAGAATCTGC TCATTGACCA GCAGGGCTAC 
ATTCAGGTGA CAGACTTCGG TTTCGCCAAG CGCGTGAAGG GCCGCACTTG GACCTTGTGC 
GGCACCCCTG AGTACCTGGC CCCTGAGATT ATCCTGAGCA AAGGCTACAA CAAGGCCGTG 
GACTGGTGGG CCCTGGGGGT TCTTATCTAT GAAATGGCCG CTGGCTACCC GCCCTTCTTC 
GCAGACCAGC CCATCCAGAT CTATGAGAAG ATCGTCTCTG GGAAGGTGCG CTTCCCTTCC 
CACTTCAGCT CTGACTTGAA GGACCTGCTG CGGAACCTCC TGCAGGTAGA TCTCACCAAG 
CGCTTTGGGA ACCTCAAGAA TGGGGTCAAC GATATCAAGA ACCACAAGTG GTTTGCCACA 
ACTGACTGGA TTGCCATCTA CCAGAGGAAG GTGGAAGCTC CCTTCATACC AAAGTTTAAA 
GGCCCTGGGG ATACGAGTAA CTTTGACGAC TATGAGGAAG AAGAAATCCG GGTCTCCATC 
AATGAGAAGT GTGGCAAGGA GTTTTCTGAG TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002721.1
CDS	201..1256
Translation	MGNAAAAKKGSEQESVKEFLAKAKEDFLKKWESPAQNTAHLDQFERIKTLGTGSFGRVMLVKHKETGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVMEYVPGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYQRKVEAPFIPKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFSEF

Target ORF information:

RefSeq Version	NM_002730.3
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase cAMP-activated catalytic subunit alpha (PRKACA), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002730.3

ORF Insert Sequence:

ATGGGCAACG CCGCCGCCGC CAAGAAGGGC AGCGAGCAGG AGAGCGTGAA AGAATTCTTA 
GCCAAAGCCA AAGAAGATTT TCTTAAAAAA TGGGAAAGTC CCGCTCAGAA CACAGCCCAC 
TTGGATCAGT TTGAACGAAT CAAGACCCTC GGCACGGGCT CCTTCGGGCG GGTGATGCTG 
GTGAAACACA AGGAGACCGG GAACCACTAT GCCATGAAGA TCCTCGACAA ACAGAAGGTG 
GTGAAACTGA AACAGATCGA ACACACCCTG AATGAAAAGC GCATCCTGCA AGCTGTCAAC 
TTTCCGTTCC TCGTCAAACT CGAGTTCTCC TTCAAGGACA ACTCAAACTT ATACATGGTC 
ATGGAGTACG TGCCCGGCGG GGAGATGTTC TCACACCTAC GGCGGATCGG AAGGTTCAGT 
GAGCCCCATG CCCGTTTCTA CGCGGCCCAG ATCGTCCTGA CCTTTGAGTA TCTGCACTCG 
CTGGATCTCA TCTACAGGGA CCTGAAGCCG GAGAATCTGC TCATTGACCA GCAGGGCTAC 
ATTCAGGTGA CAGACTTCGG TTTCGCCAAG CGCGTGAAGG GCCGCACTTG GACCTTGTGC 
GGCACCCCTG AGTACCTGGC CCCTGAGATT ATCCTGAGCA AAGGCTACAA CAAGGCCGTG 
GACTGGTGGG CCCTGGGGGT TCTTATCTAT GAAATGGCCG CTGGCTACCC GCCCTTCTTC 
GCAGACCAGC CCATCCAGAT CTATGAGAAG ATCGTCTCTG GGAAGGTGCG CTTCCCTTCC 
CACTTCAGCT CTGACTTGAA GGACCTGCTG CGGAACCTCC TGCAGGTAGA TCTCACCAAG 
CGCTTTGGGA ACCTCAAGAA TGGGGTCAAC GATATCAAGA ACCACAAGTG GTTTGCCACA 
ACTGACTGGA TTGCCATCTA CCAGAGGAAG GTGGAAGCTC CCTTCATACC AAAGTTTAAA 
GGCCCTGGGG ATACGAGTAA CTTTGACGAC TATGAGGAAG AAGAAATCCG GGTCTCCATC 
AATGAGAAGT GTGGCAAGGA GTTTTCTGAG TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKACA ( NM_002730.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002721.1 Homo sapiens protein kinase cAMP-activated catalytic subunit alpha (PRKACA), transcript variant 1, mRNA.

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CloneID	OHu18669
Clone ID Related Accession (Same CDS sequence)	NM_002730.3 , NM_002730.4
Accession Version	NM_002730.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	cAMP-dependent protein kinase catalytic subunit alpha isoform Calpha1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC022098.9, BU543573.1 and BC039846.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_002730.3. Transcript Variant: This variant (1) encodes Calpha1 (PMID:21812984). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC039846.1, X07767.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000308677.9/ ENSP00000309591.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCAACG CCGCCGCCGC CAAGAAGGGC AGCGAGCAGG AGAGCGTGAA AGAATTCTTA 
GCCAAAGCCA AAGAAGATTT TCTTAAAAAA TGGGAAAGTC CCGCTCAGAA CACAGCCCAC 
TTGGATCAGT TTGAACGAAT CAAGACCCTC GGCACGGGCT CCTTCGGGCG GGTGATGCTG 
GTGAAACACA AGGAGACCGG GAACCACTAT GCCATGAAGA TCCTCGACAA ACAGAAGGTG 
GTGAAACTGA AACAGATCGA ACACACCCTG AATGAAAAGC GCATCCTGCA AGCTGTCAAC 
TTTCCGTTCC TCGTCAAACT CGAGTTCTCC TTCAAGGACA ACTCAAACTT ATACATGGTC 
ATGGAGTACG TGCCCGGCGG GGAGATGTTC TCACACCTAC GGCGGATCGG AAGGTTCAGT 
GAGCCCCATG CCCGTTTCTA CGCGGCCCAG ATCGTCCTGA CCTTTGAGTA TCTGCACTCG 
CTGGATCTCA TCTACAGGGA CCTGAAGCCG GAGAATCTGC TCATTGACCA GCAGGGCTAC 
ATTCAGGTGA CAGACTTCGG TTTCGCCAAG CGCGTGAAGG GCCGCACTTG GACCTTGTGC 
GGCACCCCTG AGTACCTGGC CCCTGAGATT ATCCTGAGCA AAGGCTACAA CAAGGCCGTG 
GACTGGTGGG CCCTGGGGGT TCTTATCTAT GAAATGGCCG CTGGCTACCC GCCCTTCTTC 
GCAGACCAGC CCATCCAGAT CTATGAGAAG ATCGTCTCTG GGAAGGTGCG CTTCCCTTCC 
CACTTCAGCT CTGACTTGAA GGACCTGCTG CGGAACCTCC TGCAGGTAGA TCTCACCAAG 
CGCTTTGGGA ACCTCAAGAA TGGGGTCAAC GATATCAAGA ACCACAAGTG GTTTGCCACA 
ACTGACTGGA TTGCCATCTA CCAGAGGAAG GTGGAAGCTC CCTTCATACC AAAGTTTAAA 
GGCCCTGGGG ATACGAGTAA CTTTGACGAC TATGAGGAAG AAGAAATCCG GGTCTCCATC 
AATGAGAAGT GTGGCAAGGA GTTTTCTGAG TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002721.1
CDS	216..1271
Translation	MGNAAAAKKGSEQESVKEFLAKAKEDFLKKWESPAQNTAHLDQFERIKTLGTGSFGRVMLVKHKETGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVMEYVPGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYQRKVEAPFIPKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFSEF

Target ORF information:

RefSeq Version	NM_002730.4
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase cAMP-activated catalytic subunit alpha (PRKACA), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002730.4

ORF Insert Sequence:

ATGGGCAACG CCGCCGCCGC CAAGAAGGGC AGCGAGCAGG AGAGCGTGAA AGAATTCTTA 
GCCAAAGCCA AAGAAGATTT TCTTAAAAAA TGGGAAAGTC CCGCTCAGAA CACAGCCCAC 
TTGGATCAGT TTGAACGAAT CAAGACCCTC GGCACGGGCT CCTTCGGGCG GGTGATGCTG 
GTGAAACACA AGGAGACCGG GAACCACTAT GCCATGAAGA TCCTCGACAA ACAGAAGGTG 
GTGAAACTGA AACAGATCGA ACACACCCTG AATGAAAAGC GCATCCTGCA AGCTGTCAAC 
TTTCCGTTCC TCGTCAAACT CGAGTTCTCC TTCAAGGACA ACTCAAACTT ATACATGGTC 
ATGGAGTACG TGCCCGGCGG GGAGATGTTC TCACACCTAC GGCGGATCGG AAGGTTCAGT 
GAGCCCCATG CCCGTTTCTA CGCGGCCCAG ATCGTCCTGA CCTTTGAGTA TCTGCACTCG 
CTGGATCTCA TCTACAGGGA CCTGAAGCCG GAGAATCTGC TCATTGACCA GCAGGGCTAC 
ATTCAGGTGA CAGACTTCGG TTTCGCCAAG CGCGTGAAGG GCCGCACTTG GACCTTGTGC 
GGCACCCCTG AGTACCTGGC CCCTGAGATT ATCCTGAGCA AAGGCTACAA CAAGGCCGTG 
GACTGGTGGG CCCTGGGGGT TCTTATCTAT GAAATGGCCG CTGGCTACCC GCCCTTCTTC 
GCAGACCAGC CCATCCAGAT CTATGAGAAG ATCGTCTCTG GGAAGGTGCG CTTCCCTTCC 
CACTTCAGCT CTGACTTGAA GGACCTGCTG CGGAACCTCC TGCAGGTAGA TCTCACCAAG 
CGCTTTGGGA ACCTCAAGAA TGGGGTCAAC GATATCAAGA ACCACAAGTG GTTTGCCACA 
ACTGACTGGA TTGCCATCTA CCAGAGGAAG GTGGAAGCTC CCTTCATACC AAAGTTTAAA 
GGCCCTGGGG ATACGAGTAA CTTTGACGAC TATGAGGAAG AAGAAATCCG GGTCTCCATC 
AATGAGAAGT GTGGCAAGGA GTTTTCTGAG TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKACA ( NM_001304349.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001291278.1 Homo sapiens protein kinase cAMP-activated catalytic subunit alpha (PRKACA), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu51697
Clone ID Related Accession (Same CDS sequence)	NM_001304349.1
Accession Version	NM_001304349.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	cAMP-dependent protein kinase catalytic subunit alpha isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK131561.1, BC108259.1 and BC039846.1. On Jan 17, 2015 this sequence version replaced XM_005259984.1. Transcript Variant: This variant (3) contains an alternate 5' terminal exon and initiates translation at an alternate start codon, compared to variant 1. It encodes isoform 3 which has a longer and distinct N-terminus, compared to Calpha1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK131561.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2159607 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCACCT TGGGATACCA TGGGGCCGAG GAGTGGGCAT CGGGCGAGAC TTCCCTTGAA 
CCAGCTCTGG AGCAATGCCC TTGCGATTGC CACGGAGAGG AACAGGGCCA GCTGGGGGCC 
AAGGTGACAG CTGAGCCCCC CAAGGGCACC TGCTACCCGA GCCCCCGAGG CCTCCATCCC 
AGGCCCGGCC TCCCCAGAGA AGGGGCCAGA GGAACAGCCA AATCCCAAGG CTATAAATAC 
CTCGGCTGGC GTCGGCCCTG GCCATGGAAT GTCGTGAAAG AATTCTTAGC CAAAGCCAAA 
GAAGATTTTC TTAAAAAATG GGAAAGTCCC GCTCAGAACA CAGCCCACTT GGATCAGTTT 
GAACGAATCA AGACCCTCGG CACGGGCTCC TTCGGGCGGG TGATGCTGGT GAAACACAAG 
GAGACCGGGA ACCACTATGC CATGAAGATC CTCGACAAAC AGAAGGTGGT GAAACTGAAA 
CAGATCGAAC ACACCCTGAA TGAAAAGCGC ATCCTGCAAG CTGTCAACTT TCCGTTCCTC 
GTCAAACTCG AGTTCTCCTT CAAGGACAAC TCAAACTTAT ACATGGTCAT GGAGTACGTG 
CCCGGCGGGG AGATGTTCTC ACACCTACGG CGGATCGGAA GGTTCAGTGA GCCCCATGCC 
CGTTTCTACG CGGCCCAGAT CGTCCTGACC TTTGAGTATC TGCACTCGCT GGATCTCATC 
TACAGGGACC TGAAGCCGGA GAATCTGCTC ATTGACCAGC AGGGCTACAT TCAGGTGACA 
GACTTCGGTT TCGCCAAGCG CGTGAAGGGC CGCACTTGGA CCTTGTGCGG CACCCCTGAG 
TACCTGGCCC CTGAGATTAT CCTGAGCAAA GGCTACAACA AGGCCGTGGA CTGGTGGGCC 
CTGGGGGTTC TTATCTATGA AATGGCCGCT GGCTACCCGC CCTTCTTCGC AGACCAGCCC 
ATCCAGATCT ATGAGAAGAT CGTCTCTGGG AAGGTGCGCT TCCCTTCCCA CTTCAGCTCT 
GACTTGAAGG ACCTGCTGCG GAACCTCCTG CAGGTAGATC TCACCAAGCG CTTTGGGAAC 
CTCAAGAATG GGGTCAACGA TATCAAGAAC CACAAGTGGT TTGCCACAAC TGACTGGATT 
GCCATCTACC AGAGGAAGGT GGAAGCTCCC TTCATACCAA AGTTTAAAGG CCCTGGGGAT 
ACGAGTAACT TTGACGACTA TGAGGAAGAA GAAATCCGGG TCTCCATCAA TGAGAAGTGT 
GGCAAGGAGT TTTCTGAGTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001291278.1
CDS	407..1690
Translation	MFTLGYHGAEEWASGETSLEPALEQCPCDCHGEEQGQLGAKVTAEPPKGTCYPSPRGLHPRPGLPREGARGTAKSQGYKYLGWRRPWPWNVVKEFLAKAKEDFLKKWESPAQNTAHLDQFERIKTLGTGSFGRVMLVKHKETGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVMEYVPGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYQRKVEAPFIPKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFSEF

Target ORF information:

RefSeq Version	NM_001304349.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase cAMP-activated catalytic subunit alpha (PRKACA), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001304349.1

ORF Insert Sequence:

ATGTTCACCT TGGGATACCA TGGGGCCGAG GAGTGGGCAT CGGGCGAGAC TTCCCTTGAA 
CCAGCTCTGG AGCAATGCCC TTGCGATTGC CACGGAGAGG AACAGGGCCA GCTGGGGGCC 
AAGGTGACAG CTGAGCCCCC CAAGGGCACC TGCTACCCGA GCCCCCGAGG CCTCCATCCC 
AGGCCCGGCC TCCCCAGAGA AGGGGCCAGA GGAACAGCCA AATCCCAAGG CTATAAATAC 
CTCGGCTGGC GTCGGCCCTG GCCATGGAAT GTCGTGAAAG AATTCTTAGC CAAAGCCAAA 
GAAGATTTTC TTAAAAAATG GGAAAGTCCC GCTCAGAACA CAGCCCACTT GGATCAGTTT 
GAACGAATCA AGACCCTCGG CACGGGCTCC TTCGGGCGGG TGATGCTGGT GAAACACAAG 
GAGACCGGGA ACCACTATGC CATGAAGATC CTCGACAAAC AGAAGGTGGT GAAACTGAAA 
CAGATCGAAC ACACCCTGAA TGAAAAGCGC ATCCTGCAAG CTGTCAACTT TCCGTTCCTC 
GTCAAACTCG AGTTCTCCTT CAAGGACAAC TCAAACTTAT ACATGGTCAT GGAGTACGTG 
CCCGGCGGGG AGATGTTCTC ACACCTACGG CGGATCGGAA GGTTCAGTGA GCCCCATGCC 
CGTTTCTACG CGGCCCAGAT CGTCCTGACC TTTGAGTATC TGCACTCGCT GGATCTCATC 
TACAGGGACC TGAAGCCGGA GAATCTGCTC ATTGACCAGC AGGGCTACAT TCAGGTGACA 
GACTTCGGTT TCGCCAAGCG CGTGAAGGGC CGCACTTGGA CCTTGTGCGG CACCCCTGAG 
TACCTGGCCC CTGAGATTAT CCTGAGCAAA GGCTACAACA AGGCCGTGGA CTGGTGGGCC 
CTGGGGGTTC TTATCTATGA AATGGCCGCT GGCTACCCGC CCTTCTTCGC AGACCAGCCC 
ATCCAGATCT ATGAGAAGAT CGTCTCTGGG AAGGTGCGCT TCCCTTCCCA CTTCAGCTCT 
GACTTGAAGG ACCTGCTGCG GAACCTCCTG CAGGTAGATC TCACCAAGCG CTTTGGGAAC 
CTCAAGAATG GGGTCAACGA TATCAAGAAC CACAAGTGGT TTGCCACAAC TGACTGGATT 
GCCATCTACC AGAGGAAGGT GGAAGCTCCC TTCATACCAA AGTTTAAAGG CCCTGGGGAT 
ACGAGTAACT TTGACGACTA TGAGGAAGAA GAAATCCGGG TCTCCATCAA TGAGAAGTGT 
GGCAAGGAGT TTTCTGAGTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKACA ( NM_207518.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_997401.1 Homo sapiens protein kinase cAMP-activated catalytic subunit alpha (PRKACA), transcript variant 2, mRNA.

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CloneID	OHu17314
Clone ID Related Accession (Same CDS sequence)	NM_207518.2 , NM_207518.3
Accession Version	NM_207518.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	cAMP-dependent protein kinase catalytic subunit alpha isoform Calpha2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK292284.1, BC108259.1 and BC039846.1. On Jan 17, 2015 this sequence version replaced NM_207518.1. Transcript Variant: This variant (2) contains an alternate 5' terminal exon and initiates translation at an alternate start codon, compared to variant 1. It encodes Calpha2, PMID:21812984 (also known as C alpha-s, PMID: 10906071) which has a shorter and distinct N-terminus, compared to Calpha1. This variant is specifically expressed in spermatogenic cells and may be essential for male fertility. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292284.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTCCA ACTCCAGCGA TGTGAAAGAA TTCTTAGCCA AAGCCAAAGA AGATTTTCTT 
AAAAAATGGG AAAGTCCCGC TCAGAACACA GCCCACTTGG ATCAGTTTGA ACGAATCAAG 
ACCCTCGGCA CGGGCTCCTT CGGGCGGGTG ATGCTGGTGA AACACAAGGA GACCGGGAAC 
CACTATGCCA TGAAGATCCT CGACAAACAG AAGGTGGTGA AACTGAAACA GATCGAACAC 
ACCCTGAATG AAAAGCGCAT CCTGCAAGCT GTCAACTTTC CGTTCCTCGT CAAACTCGAG 
TTCTCCTTCA AGGACAACTC AAACTTATAC ATGGTCATGG AGTACGTGCC CGGCGGGGAG 
ATGTTCTCAC ACCTACGGCG GATCGGAAGG TTCAGTGAGC CCCATGCCCG TTTCTACGCG 
GCCCAGATCG TCCTGACCTT TGAGTATCTG CACTCGCTGG ATCTCATCTA CAGGGACCTG 
AAGCCGGAGA ATCTGCTCAT TGACCAGCAG GGCTACATTC AGGTGACAGA CTTCGGTTTC 
GCCAAGCGCG TGAAGGGCCG CACTTGGACC TTGTGCGGCA CCCCTGAGTA CCTGGCCCCT 
GAGATTATCC TGAGCAAAGG CTACAACAAG GCCGTGGACT GGTGGGCCCT GGGGGTTCTT 
ATCTATGAAA TGGCCGCTGG CTACCCGCCC TTCTTCGCAG ACCAGCCCAT CCAGATCTAT 
GAGAAGATCG TCTCTGGGAA GGTGCGCTTC CCTTCCCACT TCAGCTCTGA CTTGAAGGAC 
CTGCTGCGGA ACCTCCTGCA GGTAGATCTC ACCAAGCGCT TTGGGAACCT CAAGAATGGG 
GTCAACGATA TCAAGAACCA CAAGTGGTTT GCCACAACTG ACTGGATTGC CATCTACCAG 
AGGAAGGTGG AAGCTCCCTT CATACCAAAG TTTAAAGGCC CTGGGGATAC GAGTAACTTT 
GACGACTATG AGGAAGAAGA AATCCGGGTC TCCATCAATG AGAAGTGTGG CAAGGAGTTT 
TCTGAGTTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997401.1
CDS	34..1065
Translation	MASNSSDVKEFLAKAKEDFLKKWESPAQNTAHLDQFERIKTLGTGSFGRVMLVKHKETGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVMEYVPGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYQRKVEAPFIPKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFSEF

Target ORF information:

RefSeq Version	NM_207518.2
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase cAMP-activated catalytic subunit alpha (PRKACA), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_207518.2

ORF Insert Sequence:

ATGGCTTCCA ACTCCAGCGA TGTGAAAGAA TTCTTAGCCA AAGCCAAAGA AGATTTTCTT 
AAAAAATGGG AAAGTCCCGC TCAGAACACA GCCCACTTGG ATCAGTTTGA ACGAATCAAG 
ACCCTCGGCA CGGGCTCCTT CGGGCGGGTG ATGCTGGTGA AACACAAGGA GACCGGGAAC 
CACTATGCCA TGAAGATCCT CGACAAACAG AAGGTGGTGA AACTGAAACA GATCGAACAC 
ACCCTGAATG AAAAGCGCAT CCTGCAAGCT GTCAACTTTC CGTTCCTCGT CAAACTCGAG 
TTCTCCTTCA AGGACAACTC AAACTTATAC ATGGTCATGG AGTACGTGCC CGGCGGGGAG 
ATGTTCTCAC ACCTACGGCG GATCGGAAGG TTCAGTGAGC CCCATGCCCG TTTCTACGCG 
GCCCAGATCG TCCTGACCTT TGAGTATCTG CACTCGCTGG ATCTCATCTA CAGGGACCTG 
AAGCCGGAGA ATCTGCTCAT TGACCAGCAG GGCTACATTC AGGTGACAGA CTTCGGTTTC 
GCCAAGCGCG TGAAGGGCCG CACTTGGACC TTGTGCGGCA CCCCTGAGTA CCTGGCCCCT 
GAGATTATCC TGAGCAAAGG CTACAACAAG GCCGTGGACT GGTGGGCCCT GGGGGTTCTT 
ATCTATGAAA TGGCCGCTGG CTACCCGCCC TTCTTCGCAG ACCAGCCCAT CCAGATCTAT 
GAGAAGATCG TCTCTGGGAA GGTGCGCTTC CCTTCCCACT TCAGCTCTGA CTTGAAGGAC 
CTGCTGCGGA ACCTCCTGCA GGTAGATCTC ACCAAGCGCT TTGGGAACCT CAAGAATGGG 
GTCAACGATA TCAAGAACCA CAAGTGGTTT GCCACAACTG ACTGGATTGC CATCTACCAG 
AGGAAGGTGG AAGCTCCCTT CATACCAAAG TTTAAAGGCC CTGGGGATAC GAGTAACTTT 
GACGACTATG AGGAAGAAGA AATCCGGGTC TCCATCAATG AGAAGTGTGG CAAGGAGTTT 
TCTGAGTTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKACA ( NM_207518.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_997401.1 Homo sapiens protein kinase cAMP-activated catalytic subunit alpha (PRKACA), transcript variant 2, mRNA.

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CloneID	OHu17314
Clone ID Related Accession (Same CDS sequence)	NM_207518.2 , NM_207518.3
Accession Version	NM_207518.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	cAMP-dependent protein kinase catalytic subunit alpha isoform Calpha2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC022098.9, AK292284.1, BC108259.1 and BC039846.1. On May 31, 2019 this sequence version replaced NM_207518.2. Transcript Variant: This variant (2) contains an alternate 5' terminal exon and initiates translation at an alternate start codon, compared to variant 1. It encodes Calpha2, PMID:21812984 (also known as C alpha-s, PMID: 10906071) which has a shorter and distinct N-terminus, compared to Calpha1. This variant is specifically expressed in spermatogenic cells and may be essential for male fertility. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292284.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTCCA ACTCCAGCGA TGTGAAAGAA TTCTTAGCCA AAGCCAAAGA AGATTTTCTT 
AAAAAATGGG AAAGTCCCGC TCAGAACACA GCCCACTTGG ATCAGTTTGA ACGAATCAAG 
ACCCTCGGCA CGGGCTCCTT CGGGCGGGTG ATGCTGGTGA AACACAAGGA GACCGGGAAC 
CACTATGCCA TGAAGATCCT CGACAAACAG AAGGTGGTGA AACTGAAACA GATCGAACAC 
ACCCTGAATG AAAAGCGCAT CCTGCAAGCT GTCAACTTTC CGTTCCTCGT CAAACTCGAG 
TTCTCCTTCA AGGACAACTC AAACTTATAC ATGGTCATGG AGTACGTGCC CGGCGGGGAG 
ATGTTCTCAC ACCTACGGCG GATCGGAAGG TTCAGTGAGC CCCATGCCCG TTTCTACGCG 
GCCCAGATCG TCCTGACCTT TGAGTATCTG CACTCGCTGG ATCTCATCTA CAGGGACCTG 
AAGCCGGAGA ATCTGCTCAT TGACCAGCAG GGCTACATTC AGGTGACAGA CTTCGGTTTC 
GCCAAGCGCG TGAAGGGCCG CACTTGGACC TTGTGCGGCA CCCCTGAGTA CCTGGCCCCT 
GAGATTATCC TGAGCAAAGG CTACAACAAG GCCGTGGACT GGTGGGCCCT GGGGGTTCTT 
ATCTATGAAA TGGCCGCTGG CTACCCGCCC TTCTTCGCAG ACCAGCCCAT CCAGATCTAT 
GAGAAGATCG TCTCTGGGAA GGTGCGCTTC CCTTCCCACT TCAGCTCTGA CTTGAAGGAC 
CTGCTGCGGA ACCTCCTGCA GGTAGATCTC ACCAAGCGCT TTGGGAACCT CAAGAATGGG 
GTCAACGATA TCAAGAACCA CAAGTGGTTT GCCACAACTG ACTGGATTGC CATCTACCAG 
AGGAAGGTGG AAGCTCCCTT CATACCAAAG TTTAAAGGCC CTGGGGATAC GAGTAACTTT 
GACGACTATG AGGAAGAAGA AATCCGGGTC TCCATCAATG AGAAGTGTGG CAAGGAGTTT 
TCTGAGTTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997401.1
CDS	37..1068
Translation	MASNSSDVKEFLAKAKEDFLKKWESPAQNTAHLDQFERIKTLGTGSFGRVMLVKHKETGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVMEYVPGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYQRKVEAPFIPKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFSEF

Target ORF information:

RefSeq Version	NM_207518.3
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase cAMP-activated catalytic subunit alpha (PRKACA), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_207518.3

ORF Insert Sequence:

ATGGCTTCCA ACTCCAGCGA TGTGAAAGAA TTCTTAGCCA AAGCCAAAGA AGATTTTCTT 
AAAAAATGGG AAAGTCCCGC TCAGAACACA GCCCACTTGG ATCAGTTTGA ACGAATCAAG 
ACCCTCGGCA CGGGCTCCTT CGGGCGGGTG ATGCTGGTGA AACACAAGGA GACCGGGAAC 
CACTATGCCA TGAAGATCCT CGACAAACAG AAGGTGGTGA AACTGAAACA GATCGAACAC 
ACCCTGAATG AAAAGCGCAT CCTGCAAGCT GTCAACTTTC CGTTCCTCGT CAAACTCGAG 
TTCTCCTTCA AGGACAACTC AAACTTATAC ATGGTCATGG AGTACGTGCC CGGCGGGGAG 
ATGTTCTCAC ACCTACGGCG GATCGGAAGG TTCAGTGAGC CCCATGCCCG TTTCTACGCG 
GCCCAGATCG TCCTGACCTT TGAGTATCTG CACTCGCTGG ATCTCATCTA CAGGGACCTG 
AAGCCGGAGA ATCTGCTCAT TGACCAGCAG GGCTACATTC AGGTGACAGA CTTCGGTTTC 
GCCAAGCGCG TGAAGGGCCG CACTTGGACC TTGTGCGGCA CCCCTGAGTA CCTGGCCCCT 
GAGATTATCC TGAGCAAAGG CTACAACAAG GCCGTGGACT GGTGGGCCCT GGGGGTTCTT 
ATCTATGAAA TGGCCGCTGG CTACCCGCCC TTCTTCGCAG ACCAGCCCAT CCAGATCTAT 
GAGAAGATCG TCTCTGGGAA GGTGCGCTTC CCTTCCCACT TCAGCTCTGA CTTGAAGGAC 
CTGCTGCGGA ACCTCCTGCA GGTAGATCTC ACCAAGCGCT TTGGGAACCT CAAGAATGGG 
GTCAACGATA TCAAGAACCA CAAGTGGTTT GCCACAACTG ACTGGATTGC CATCTACCAG 
AGGAAGGTGG AAGCTCCCTT CATACCAAAG TTTAAAGGCC CTGGGGATAC GAGTAACTTT 
GACGACTATG AGGAAGAAGA AATCCGGGTC TCCATCAATG AGAAGTGTGG CAAGGAGTTT 
TCTGAGTTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKACA ( XM_017026948.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016882437.1 Homo sapiens protein kinase cAMP-activated catalytic subunit alpha (PRKACA), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu88549
Clone ID Related Accession (Same CDS sequence)	XM_017026948.1
Accession Version	XM_017026948.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 891bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cAMP-dependent protein kinase catalytic subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAACG CCGCCGCCGC CAAGAAGGGC AGCGAGCAGG AGAGCGTGAA AGAATTCTTA 
GCCAAAGCCA AAGAAGATTT TCTTAAAAAA TGGGAAAGTC CCGCTCAGAA CACAGCCCAC 
TTGGATCAGT TTGAACGAAT CAAGACCCTC GGCACGGGCT CCTTCGGGCG GGTGATGCTG 
GTGAAACACA AGGAGACCGG GAACCACTAT GCCATGAAGA TCCTCGACAA ACAGAAGGTG 
GTGAAACTGA AACAGATCGA ACACACCCTG AATGAAAAGC GCATCCTGCA AGCTGTCAAC 
TTTCCGTTCC TCGTCAAACT CGAGTTCTCC TTCAAGGACA ACTCAAACTT ATACATGGTC 
ATGGAGTACG TGCCCGGCGG GGAGATGTTC TCACACCTAC GGCGGATCGG AAGGTTCAGT 
GAGCCCCATG CCCGTTTCTA CGCGGCCCAG ATCGTCCTGA CCTTTGAGTA TCTGCACTCG 
CTGGATCTCA TCTACAGGGA CCTGAAGCCG GAGAATCTGC TCATTGACCA GCAGGGCTAC 
ATTCAGGTGA CAGACTTCGG TTTCGCCAAG CGCGTGAAGG GCCGCACTTG GACCTTGTGC 
GGCACCCCTG AGTACCTGGC CCCTGAGATT ATCCTGAGCA AAGGCTACAA CAAGGCCGTG 
GACTGGTGGG CCCTGGGGGT TCTTATCTAT GAAATGGCCG CTGGCTACCC GCCCTTCTTC 
GCAGACCAGC CCATCCAGAT CTATGAGAAG ATCGTCTCTG GGAAGGTGGA AGCTCCCTTC 
ATACCAAAGT TTAAAGGCCC TGGGGATACG AGTAACTTTG ACGACTATGA GGAAGAAGAA 
ATCCGGGTCT CCATCAATGA GAAGTGTGGC AAGGAGTTTT CTGAGTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016882437.1
CDS	57..947
Translation	MGNAAAAKKGSEQESVKEFLAKAKEDFLKKWESPAQNTAHLDQFERIKTLGTGSFGRVMLVKHKETGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVMEYVPGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVEAPFIPKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFSEF

Target ORF information:

RefSeq Version	XM_017026948.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase cAMP-activated catalytic subunit alpha (PRKACA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017026948.1

ORF Insert Sequence:

ATGGGCAACG CCGCCGCCGC CAAGAAGGGC AGCGAGCAGG AGAGCGTGAA AGAATTCTTA 
GCCAAAGCCA AAGAAGATTT TCTTAAAAAA TGGGAAAGTC CCGCTCAGAA CACAGCCCAC 
TTGGATCAGT TTGAACGAAT CAAGACCCTC GGCACGGGCT CCTTCGGGCG GGTGATGCTG 
GTGAAACACA AGGAGACCGG GAACCACTAT GCCATGAAGA TCCTCGACAA ACAGAAGGTG 
GTGAAACTGA AACAGATCGA ACACACCCTG AATGAAAAGC GCATCCTGCA AGCTGTCAAC 
TTTCCGTTCC TCGTCAAACT CGAGTTCTCC TTCAAGGACA ACTCAAACTT ATACATGGTC 
ATGGAGTACG TGCCCGGCGG GGAGATGTTC TCACACCTAC GGCGGATCGG AAGGTTCAGT 
GAGCCCCATG CCCGTTTCTA CGCGGCCCAG ATCGTCCTGA CCTTTGAGTA TCTGCACTCG 
CTGGATCTCA TCTACAGGGA CCTGAAGCCG GAGAATCTGC TCATTGACCA GCAGGGCTAC 
ATTCAGGTGA CAGACTTCGG TTTCGCCAAG CGCGTGAAGG GCCGCACTTG GACCTTGTGC 
GGCACCCCTG AGTACCTGGC CCCTGAGATT ATCCTGAGCA AAGGCTACAA CAAGGCCGTG 
GACTGGTGGG CCCTGGGGGT TCTTATCTAT GAAATGGCCG CTGGCTACCC GCCCTTCTTC 
GCAGACCAGC CCATCCAGAT CTATGAGAAG ATCGTCTCTG GGAAGGTGGA AGCTCCCTTC 
ATACCAAAGT TTAAAGGCCC TGGGGATACG AGTAACTTTG ACGACTATGA GGAAGAAGAA 
ATCCGGGTCT CCATCAATGA GAAGTGTGGC AAGGAGTTTT CTGAGTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Constitutive activation of PKA catalytic subunit in adrenal Cushing's syndrome.
The New England journal of medicine370(11)1019-28(2014 Mar)
Beuschlein F,Fassnacht M,Assi? G,Calebiro D,Stratakis CA,Osswald A,Ronchi CL,Wieland T,Sbiera S,Faucz FR,Schaak K,Schmittfull A,Schwarzmayr T,Barreau O,Vezzosi D,Rizk-Rabin M,Zabel U,Szarek E,Salpea P,Forlino A,Vetro A,Zuffardi O,Kisker C,Diener S,Meitinger T,Lohse MJ,Reincke M,Bertherat J,Strom TM,Allolio B

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PRKACA cDNA ORF clone, Homo sapiens(human)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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