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PRKACA cDNA ORF clone, Homo sapiens (human)

Gene Symbol PRKACA
Entrez Gene ID 5566
Full Name protein kinase, cAMP-dependent, catalytic, alpha
Synonyms PKACA, PPNAD4
General protein information
Preferred Names
cAMP-dependent protein kinase catalytic subunit alpha
Names
cAMP-dependent protein kinase catalytic subunit alpha
PKA C-alpha
protein kinase A catalytic subunit
cAMP-dependent protein kinase catalytic subunit alpha, isoform 1
NP_002721.1
NP_997401.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

19

19p13.1

Summary This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms. [provided by RefSeq, Jan 2015]. lac of sum
Disorder MIM:

601639


mRNA and Protein(s)

mRNA Protein Name
NM_207518 NP_997401 cAMP-dependent protein kinase catalytic subunit alpha isoform Calpha2
NM_001304349 NP_001291278 cAMP-dependent protein kinase catalytic subunit alpha isoform 3
NM_002730 NP_002721 cAMP-dependent protein kinase catalytic subunit alpha isoform Calpha1



Homo sapiens (human) PRKACA NP_002721.1
Pan troglodytes (chimpanzee) PRKACA XP_001171437.1
Canis lupus familiaris (dog) PRKACA NP_001003032.1
Bos taurus (cattle) PRKACA NP_777009.1
Mus musculus (house mouse) Prkaca NP_032880.1
Rattus norvegicus (Norway rat) Prkaca NP_001094392.1
Danio rerio (zebrafish) prkacab NP_001003470.1
Xenopus (Silurana) tropicalis (western clawed frog) prkaca NP_001016403.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PRKACA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRKACA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu17314 NM_207518 Homo sapiens protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $202.30
$289.00
OHu51697 NM_001304349 Homo sapiens protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu18669
NM_002730 Homo sapiens protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu17314
Clone ID Related Accession (Same CDS sequence) NM_207518
Accession Version NM_207518.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1032bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cAMP-dependent protein kinase catalytic subunit alpha isoform Calpha2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK292284.1, BC108259.1 and BC039846.1. On Jan 17, 2015 this sequence version replaced gi:46909583. Transcript Variant: This variant (2) contains an alternate 5' terminal exon and initiates translation at an alternate start codon, compared to variant 1. It encodes Calpha2, PMID:21812984 (also known as C alpha-s, PMID: 10906071) which has a shorter and distinct N-terminus, compared to Calpha1. This variant is specifically expressed in spermatogenic cells and may be essential for male fertility. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292284.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA962337, SAMEA962343 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCTTCCA ACTCCAGCGA TGTGAAAGAA TTCTTAGCCA AAGCCAAAGA AGATTTTCTT 
AAAAAATGGG AAAGTCCCGC TCAGAACACA GCCCACTTGG ATCAGTTTGA ACGAATCAAG
ACCCTCGGCA CGGGCTCCTT CGGGCGGGTG ATGCTGGTGA AACACAAGGA GACCGGGAAC
CACTATGCCA TGAAGATCCT CGACAAACAG AAGGTGGTGA AACTGAAACA GATCGAACAC
ACCCTGAATG AAAAGCGCAT CCTGCAAGCT GTCAACTTTC CGTTCCTCGT CAAACTCGAG
TTCTCCTTCA AGGACAACTC AAACTTATAC ATGGTCATGG AGTACGTGCC CGGCGGGGAG
ATGTTCTCAC ACCTACGGCG GATCGGAAGG TTCAGTGAGC CCCATGCCCG TTTCTACGCG
GCCCAGATCG TCCTGACCTT TGAGTATCTG CACTCGCTGG ATCTCATCTA CAGGGACCTG
AAGCCGGAGA ATCTGCTCAT TGACCAGCAG GGCTACATTC AGGTGACAGA CTTCGGTTTC
GCCAAGCGCG TGAAGGGCCG CACTTGGACC TTGTGCGGCA CCCCTGAGTA CCTGGCCCCT
GAGATTATCC TGAGCAAAGG CTACAACAAG GCCGTGGACT GGTGGGCCCT GGGGGTTCTT
ATCTATGAAA TGGCCGCTGG CTACCCGCCC TTCTTCGCAG ACCAGCCCAT CCAGATCTAT
GAGAAGATCG TCTCTGGGAA GGTGCGCTTC CCTTCCCACT TCAGCTCTGA CTTGAAGGAC
CTGCTGCGGA ACCTCCTGCA GGTAGATCTC ACCAAGCGCT TTGGGAACCT CAAGAATGGG
GTCAACGATA TCAAGAACCA CAAGTGGTTT GCCACAACTG ACTGGATTGC CATCTACCAG
AGGAAGGTGG AAGCTCCCTT CATACCAAAG TTTAAAGGCC CTGGGGATAC GAGTAACTTT
GACGACTATG AGGAAGAAGA AATCCGGGTC TCCATCAATG AGAAGTGTGG CAAGGAGTTT
TCTGAGTTTT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_997401.1
CDS34..1065
Misc Feature(1)28..30(+)
Misc Feature(2)133..1002(+)
Misc Feature(3)139..903(+)
Misc Feature(4)157..993(+)
Misc Feature(5)160..1002(+)
Misc Feature(6)163..1002(+)
Misc Feature(7)169..1002(+)
Misc Feature(8)427..429(+)
Misc Feature(9)559..621(+)
Misc Feature(10)595..597(+)
Misc Feature(11)601..603(+)
Misc Feature(12)613..615(+)
Misc Feature(13)1024..1026(+)
Exon (1)1..55
Gene:PRKACA
Gene Synonym:
Exon (2)56..117
Gene:PRKACA
Gene Synonym:
Exon (3)118..246
Gene:PRKACA
Gene Synonym:
Exon (4)247..345
Gene:PRKACA
Gene Synonym:
Exon (5)346..428
Gene:PRKACA
Gene Synonym:
Exon (6)429..555
Gene:PRKACA
Gene Synonym:
Exon (7)556..651
Gene:PRKACA
Gene Synonym:
Exon (8)652..774
Gene:PRKACA
Gene Synonym:
Exon (9)775..939
Gene:PRKACA
Gene Synonym:
Exon (10)940..2489
Gene:PRKACA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_207518
Organism Homo sapiens (human)
Definition Homo sapiens protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_207518

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCTTCCA ACTCCAGCGA TGTGAAAGAA TTCTTAGCCA AAGCCAAAGA AGATTTTCTT 
AAAAAATGGG AAAGTCCCGC TCAGAACACA GCCCACTTGG ATCAGTTTGA ACGAATCAAG
ACCCTCGGCA CGGGCTCCTT CGGGCGGGTG ATGCTGGTGA AACACAAGGA GACCGGGAAC
CACTATGCCA TGAAGATCCT CGACAAACAG AAGGTGGTGA AACTGAAACA GATCGAACAC
ACCCTGAATG AAAAGCGCAT CCTGCAAGCT GTCAACTTTC CGTTCCTCGT CAAACTCGAG
TTCTCCTTCA AGGACAACTC AAACTTATAC ATGGTCATGG AGTACGTGCC CGGCGGGGAG
ATGTTCTCAC ACCTACGGCG GATCGGAAGG TTCAGTGAGC CCCATGCCCG TTTCTACGCG
GCCCAGATCG TCCTGACCTT TGAGTATCTG CACTCGCTGG ATCTCATCTA CAGGGACCTG
AAGCCGGAGA ATCTGCTCAT TGACCAGCAG GGCTACATTC AGGTGACAGA CTTCGGTTTC
GCCAAGCGCG TGAAGGGCCG CACTTGGACC TTGTGCGGCA CCCCTGAGTA CCTGGCCCCT
GAGATTATCC TGAGCAAAGG CTACAACAAG GCCGTGGACT GGTGGGCCCT GGGGGTTCTT
ATCTATGAAA TGGCCGCTGG CTACCCGCCC TTCTTCGCAG ACCAGCCCAT CCAGATCTAT
GAGAAGATCG TCTCTGGGAA GGTGCGCTTC CCTTCCCACT TCAGCTCTGA CTTGAAGGAC
CTGCTGCGGA ACCTCCTGCA GGTAGATCTC ACCAAGCGCT TTGGGAACCT CAAGAATGGG
GTCAACGATA TCAAGAACCA CAAGTGGTTT GCCACAACTG ACTGGATTGC CATCTACCAG
AGGAAGGTGG AAGCTCCCTT CATACCAAAG TTTAAAGGCC CTGGGGATAC GAGTAACTTT
GACGACTATG AGGAAGAAGA AATCCGGGTC TCCATCAATG AGAAGTGTGG CAAGGAGTTT
TCTGAGTTTT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu51697
Clone ID Related Accession (Same CDS sequence) NM_001304349
Accession Version NM_001304349.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1284bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cAMP-dependent protein kinase catalytic subunit alpha isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK131561.1, BC108259.1 and BC039846.1. On Jan 17, 2015 this sequence version replaced gi:530415007. Transcript Variant: This variant (3) contains an alternate 5' terminal exon and initiates translation at an alternate start codon, compared to variant 1. It encodes isoform 3 which has a longer and distinct N-terminus, compared to Calpha1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK131561.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2159607, SAMEA962337 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGTTCACCT TGGGATACCA TGGGGCCGAG GAGTGGGCAT CGGGCGAGAC TTCCCTTGAA 
CCAGCTCTGG AGCAATGCCC TTGCGATTGC CACGGAGAGG AACAGGGCCA GCTGGGGGCC
AAGGTGACAG CTGAGCCCCC CAAGGGCACC TGCTACCCGA GCCCCCGAGG CCTCCATCCC
AGGCCCGGCC TCCCCAGAGA AGGGGCCAGA GGAACAGCCA AATCCCAAGG CTATAAATAC
CTCGGCTGGC GTCGGCCCTG GCCATGGAAT GTCGTGAAAG AATTCTTAGC CAAAGCCAAA
GAAGATTTTC TTAAAAAATG GGAAAGTCCC GCTCAGAACA CAGCCCACTT GGATCAGTTT
GAACGAATCA AGACCCTCGG CACGGGCTCC TTCGGGCGGG TGATGCTGGT GAAACACAAG
GAGACCGGGA ACCACTATGC CATGAAGATC CTCGACAAAC AGAAGGTGGT GAAACTGAAA
CAGATCGAAC ACACCCTGAA TGAAAAGCGC ATCCTGCAAG CTGTCAACTT TCCGTTCCTC
GTCAAACTCG AGTTCTCCTT CAAGGACAAC TCAAACTTAT ACATGGTCAT GGAGTACGTG
CCCGGCGGGG AGATGTTCTC ACACCTACGG CGGATCGGAA GGTTCAGTGA GCCCCATGCC
CGTTTCTACG CGGCCCAGAT CGTCCTGACC TTTGAGTATC TGCACTCGCT GGATCTCATC
TACAGGGACC TGAAGCCGGA GAATCTGCTC ATTGACCAGC AGGGCTACAT TCAGGTGACA
GACTTCGGTT TCGCCAAGCG CGTGAAGGGC CGCACTTGGA CCTTGTGCGG CACCCCTGAG
TACCTGGCCC CTGAGATTAT CCTGAGCAAA GGCTACAACA AGGCCGTGGA CTGGTGGGCC
CTGGGGGTTC TTATCTATGA AATGGCCGCT GGCTACCCGC CCTTCTTCGC AGACCAGCCC
ATCCAGATCT ATGAGAAGAT CGTCTCTGGG AAGGTGCGCT TCCCTTCCCA CTTCAGCTCT
GACTTGAAGG ACCTGCTGCG GAACCTCCTG CAGGTAGATC TCACCAAGCG CTTTGGGAAC
CTCAAGAATG GGGTCAACGA TATCAAGAAC CACAAGTGGT TTGCCACAAC TGACTGGATT
GCCATCTACC AGAGGAAGGT GGAAGCTCCC TTCATACCAA AGTTTAAAGG CCCTGGGGAT
ACGAGTAACT TTGACGACTA TGAGGAAGAA GAAATCCGGG TCTCCATCAA TGAGAAGTGT
GGCAAGGAGT TTTCTGAGTT TTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001291278.1
CDS407..1690
Misc Feature(1)341..343(+)
Misc Feature(2)758..1627(+)
Misc Feature(3)764..1528(+)
Misc Feature(4)782..1618(+)
Misc Feature(5)785..1627(+)
Misc Feature(6)788..1627(+)
Misc Feature(7)794..1627(+)
Misc Feature(8)1184..1246(+)
Exon (1)1..680
Gene:PRKACA
Gene Synonym:
Exon (2)681..742
Gene:PRKACA
Gene Synonym:
Exon (3)743..871
Gene:PRKACA
Gene Synonym:
Exon (4)872..970
Gene:PRKACA
Gene Synonym:
Exon (5)971..1053
Gene:PRKACA
Gene Synonym:
Exon (6)1054..1180
Gene:PRKACA
Gene Synonym:
Exon (7)1181..1276
Gene:PRKACA
Gene Synonym:
Exon (8)1277..1399
Gene:PRKACA
Gene Synonym:
Exon (9)1400..1564
Gene:PRKACA
Gene Synonym:
Exon (10)1565..3114
Gene:PRKACA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001304349
Organism Homo sapiens (human)
Definition Homo sapiens protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001304349

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGTTCACCT TGGGATACCA TGGGGCCGAG GAGTGGGCAT CGGGCGAGAC TTCCCTTGAA 
CCAGCTCTGG AGCAATGCCC TTGCGATTGC CACGGAGAGG AACAGGGCCA GCTGGGGGCC
AAGGTGACAG CTGAGCCCCC CAAGGGCACC TGCTACCCGA GCCCCCGAGG CCTCCATCCC
AGGCCCGGCC TCCCCAGAGA AGGGGCCAGA GGAACAGCCA AATCCCAAGG CTATAAATAC
CTCGGCTGGC GTCGGCCCTG GCCATGGAAT GTCGTGAAAG AATTCTTAGC CAAAGCCAAA
GAAGATTTTC TTAAAAAATG GGAAAGTCCC GCTCAGAACA CAGCCCACTT GGATCAGTTT
GAACGAATCA AGACCCTCGG CACGGGCTCC TTCGGGCGGG TGATGCTGGT GAAACACAAG
GAGACCGGGA ACCACTATGC CATGAAGATC CTCGACAAAC AGAAGGTGGT GAAACTGAAA
CAGATCGAAC ACACCCTGAA TGAAAAGCGC ATCCTGCAAG CTGTCAACTT TCCGTTCCTC
GTCAAACTCG AGTTCTCCTT CAAGGACAAC TCAAACTTAT ACATGGTCAT GGAGTACGTG
CCCGGCGGGG AGATGTTCTC ACACCTACGG CGGATCGGAA GGTTCAGTGA GCCCCATGCC
CGTTTCTACG CGGCCCAGAT CGTCCTGACC TTTGAGTATC TGCACTCGCT GGATCTCATC
TACAGGGACC TGAAGCCGGA GAATCTGCTC ATTGACCAGC AGGGCTACAT TCAGGTGACA
GACTTCGGTT TCGCCAAGCG CGTGAAGGGC CGCACTTGGA CCTTGTGCGG CACCCCTGAG
TACCTGGCCC CTGAGATTAT CCTGAGCAAA GGCTACAACA AGGCCGTGGA CTGGTGGGCC
CTGGGGGTTC TTATCTATGA AATGGCCGCT GGCTACCCGC CCTTCTTCGC AGACCAGCCC
ATCCAGATCT ATGAGAAGAT CGTCTCTGGG AAGGTGCGCT TCCCTTCCCA CTTCAGCTCT
GACTTGAAGG ACCTGCTGCG GAACCTCCTG CAGGTAGATC TCACCAAGCG CTTTGGGAAC
CTCAAGAATG GGGTCAACGA TATCAAGAAC CACAAGTGGT TTGCCACAAC TGACTGGATT
GCCATCTACC AGAGGAAGGT GGAAGCTCCC TTCATACCAA AGTTTAAAGG CCCTGGGGAT
ACGAGTAACT TTGACGACTA TGAGGAAGAA GAAATCCGGG TCTCCATCAA TGAGAAGTGT
GGCAAGGAGT TTTCTGAGTT TTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18669
Clone ID Related Accession (Same CDS sequence) NM_002730
Accession Version NM_002730.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1056bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cAMP-dependent protein kinase catalytic subunit alpha isoform Calpha1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU543573.1 and BC039846.1. This sequence is a reference standard in the RefSeqGene project. On Apr 30, 2004 this sequence version replaced gi:31543437. Transcript Variant: This variant (1) encodes Calpha1 (PMID:21812984). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC108259.1, X07767.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA962346 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGGCAACG CCGCCGCCGC CAAGAAGGGC AGCGAGCAGG AGAGCGTGAA AGAATTCTTA 
GCCAAAGCCA AAGAAGATTT TCTTAAAAAA TGGGAAAGTC CCGCTCAGAA CACAGCCCAC
TTGGATCAGT TTGAACGAAT CAAGACCCTC GGCACGGGCT CCTTCGGGCG GGTGATGCTG
GTGAAACACA AGGAGACCGG GAACCACTAT GCCATGAAGA TCCTCGACAA ACAGAAGGTG
GTGAAACTGA AACAGATCGA ACACACCCTG AATGAAAAGC GCATCCTGCA AGCTGTCAAC
TTTCCGTTCC TCGTCAAACT CGAGTTCTCC TTCAAGGACA ACTCAAACTT ATACATGGTC
ATGGAGTACG TGCCCGGCGG GGAGATGTTC TCACACCTAC GGCGGATCGG AAGGTTCAGT
GAGCCCCATG CCCGTTTCTA CGCGGCCCAG ATCGTCCTGA CCTTTGAGTA TCTGCACTCG
CTGGATCTCA TCTACAGGGA CCTGAAGCCG GAGAATCTGC TCATTGACCA GCAGGGCTAC
ATTCAGGTGA CAGACTTCGG TTTCGCCAAG CGCGTGAAGG GCCGCACTTG GACCTTGTGC
GGCACCCCTG AGTACCTGGC CCCTGAGATT ATCCTGAGCA AAGGCTACAA CAAGGCCGTG
GACTGGTGGG CCCTGGGGGT TCTTATCTAT GAAATGGCCG CTGGCTACCC GCCCTTCTTC
GCAGACCAGC CCATCCAGAT CTATGAGAAG ATCGTCTCTG GGAAGGTGCG CTTCCCTTCC
CACTTCAGCT CTGACTTGAA GGACCTGCTG CGGAACCTCC TGCAGGTAGA TCTCACCAAG
CGCTTTGGGA ACCTCAAGAA TGGGGTCAAC GATATCAAGA ACCACAAGTG GTTTGCCACA
ACTGACTGGA TTGCCATCTA CCAGAGGAAG GTGGAAGCTC CCTTCATACC AAAGTTTAAA
GGCCCTGGGG ATACGAGTAA CTTTGACGAC TATGAGGAAG AAGAAATCCG GGTCTCCATC
AATGAGAAGT GTGGCAAGGA GTTTTCTGAG TTTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002721.1
CDS201..1256
Misc Feature(1)207..209(+)
Misc Feature(2)231..233(+)
Misc Feature(3)231..233(+)
Misc Feature(4)324..1193(+)
Misc Feature(5)330..1094(+)
Misc Feature(6)345..347(+)
Misc Feature(7)348..1184(+)
Misc Feature(8)351..1193(+)
Misc Feature(9)354..1193(+)
Misc Feature(10)360..1193(+)
Misc Feature(11)618..620(+)
Misc Feature(12)618..620(+)
Misc Feature(13)750..812(+)
Misc Feature(14)786..788(+)
Misc Feature(15)786..788(+)
Misc Feature(16)792..794(+)
Misc Feature(17)792..794(+)
Misc Feature(18)804..806(+)
Misc Feature(19)804..806(+)
Misc Feature(20)1191..1193(+)
Misc Feature(21)1215..1217(+)
Misc Feature(22)1215..1217(+)
Exon (1)1..246
Gene:PRKACA
Gene Synonym:
Exon (2)247..308
Gene:PRKACA
Gene Synonym:
Exon (3)309..437
Gene:PRKACA
Gene Synonym:
Exon (4)438..536
Gene:PRKACA
Gene Synonym:
Exon (5)537..619
Gene:PRKACA
Gene Synonym:
Exon (6)620..746
Gene:PRKACA
Gene Synonym:
Exon (7)747..842
Gene:PRKACA
Gene Synonym:
Exon (8)843..965
Gene:PRKACA
Gene Synonym:
Exon (9)966..1130
Gene:PRKACA
Gene Synonym:
Exon (10)1131..2680
Gene:PRKACA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_002730
Organism Homo sapiens (human)
Definition Homo sapiens protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002730

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGGCAACG CCGCCGCCGC CAAGAAGGGC AGCGAGCAGG AGAGCGTGAA AGAATTCTTA 
GCCAAAGCCA AAGAAGATTT TCTTAAAAAA TGGGAAAGTC CCGCTCAGAA CACAGCCCAC
TTGGATCAGT TTGAACGAAT CAAGACCCTC GGCACGGGCT CCTTCGGGCG GGTGATGCTG
GTGAAACACA AGGAGACCGG GAACCACTAT GCCATGAAGA TCCTCGACAA ACAGAAGGTG
GTGAAACTGA AACAGATCGA ACACACCCTG AATGAAAAGC GCATCCTGCA AGCTGTCAAC
TTTCCGTTCC TCGTCAAACT CGAGTTCTCC TTCAAGGACA ACTCAAACTT ATACATGGTC
ATGGAGTACG TGCCCGGCGG GGAGATGTTC TCACACCTAC GGCGGATCGG AAGGTTCAGT
GAGCCCCATG CCCGTTTCTA CGCGGCCCAG ATCGTCCTGA CCTTTGAGTA TCTGCACTCG
CTGGATCTCA TCTACAGGGA CCTGAAGCCG GAGAATCTGC TCATTGACCA GCAGGGCTAC
ATTCAGGTGA CAGACTTCGG TTTCGCCAAG CGCGTGAAGG GCCGCACTTG GACCTTGTGC
GGCACCCCTG AGTACCTGGC CCCTGAGATT ATCCTGAGCA AAGGCTACAA CAAGGCCGTG
GACTGGTGGG CCCTGGGGGT TCTTATCTAT GAAATGGCCG CTGGCTACCC GCCCTTCTTC
GCAGACCAGC CCATCCAGAT CTATGAGAAG ATCGTCTCTG GGAAGGTGCG CTTCCCTTCC
CACTTCAGCT CTGACTTGAA GGACCTGCTG CGGAACCTCC TGCAGGTAGA TCTCACCAAG
CGCTTTGGGA ACCTCAAGAA TGGGGTCAAC GATATCAAGA ACCACAAGTG GTTTGCCACA
ACTGACTGGA TTGCCATCTA CCAGAGGAAG GTGGAAGCTC CCTTCATACC AAAGTTTAAA
GGCCCTGGGG ATACGAGTAA CTTTGACGAC TATGAGGAAG AAGAAATCCG GGTCTCCATC
AATGAGAAGT GTGGCAAGGA GTTTTCTGAG TTTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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Recurrent activating mutation in PRKACA in cortisol-producing adrenal tumors
Nat. Genet. 46 (6), 613-617 (2014)
Goh G, Scholl UI, Healy JM, Choi M, Prasad ML, Nelson-Williams C, Kunstman JW, Korah R, Suttorp AC, Dietrich D, Haase M, Willenberg HS, Stalberg P, Hellman P, Akerstrom G, Bjorklund P, Carling T and Lifton RP.


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Recurrent somatic mutations underlie corticotropin-independent Cushing's syndrome
Science 344 (6186), 917-920 (2014)
Sato Y, Maekawa S, Ishii R, Sanada M, Morikawa T, Shiraishi Y, Yoshida K, Nagata Y, Sato-Otsubo A, Yoshizato T, Suzuki H, Shiozawa Y, Kataoka K, Kon A, Aoki K, Chiba K, Tanaka H, Kume H, Miyano S, Fukayama M, Nureki O, Homma Y and Ogawa S.


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Activating hotspot L205R mutation in PRKACA and adrenal Cushing's syndrome
Science 344 (6186), 913-917 (2014)
Cao Y, He M, Gao Z, Peng Y, Li Y, Li L, Zhou W, Li X, Zhong X, Lei Y, Su T, Wang H, Jiang Y, Yang L, Wei W, Yang X, Jiang X, Liu L, He J, Ye J, Wei Q, Li Y, Wang W, Wang J and Ning G.


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Constitutive activation of PKA catalytic subunit in adrenal Cushing's syndrome
N. Engl. J. Med. 370 (11), 1019-1028 (2014)
Beuschlein F, Fassnacht M, Assie G, Calebiro D, Stratakis CA, Osswald A, Ronchi CL, Wieland T, Sbiera S, Faucz FR, Schaak K, Schmittfull A, Schwarzmayr T, Barreau O, Vezzosi D, Rizk-Rabin M, Zabel U, Szarek E, Salpea P, Forlino A, Vetro A, Zuffardi O, Kisker C, Diener S, Meitinger T, Lohse MJ, Reincke M, Bertherat J, Strom TM and Allolio B.


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The unique catalytic subunit of sperm cAMP-dependent protein kinase is the product of an alternative Calpha mRNA expressed specifically in spermatogenic cells
Mol. Biol. Cell 11 (9), 3031-3044 (2000)
Agustin JT, Wilkerson CG and Witman GB.


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A novel isoform of human cyclic 3',5'-adenosine monophosphate-dependent protein kinase, c alpha-s, localizes to sperm midpiece
Biol. Reprod. 63 (2), 607-611 (2000)
Reinton N, Orstavik S, Haugen TB, Jahnsen T, Tasken K and Skalhegg BS.


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Expression of a nonmyristylated variant of the catalytic subunit of protein kinase A during male germ-cell development
Proc. Natl. Acad. Sci. U.S.A. 97 (12), 6433-6438 (2000)
Desseyn JL, Burton KA and McKnight GS.


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A cDNA clone encoding human cAMP-dependent protein kinase catalytic subunit C alpha
Nucleic Acids Res. 16 (16), 8189-8190 (1988)
Maldonado F and Hanks SK.


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Sequence of two phosphorylated sites in the catalytic subunit of bovine cardiac muscle adenosine 3':5'-monophosphate-dependent protein kinase
J. Biol. Chem. 254 (14), 6211-6214 (1979)
Shoji,S., Titani,K., Demaille,J.G. and Fischer,E.H.


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Direct phosphorylation of brain tyrosine hydroxylase by cyclic AMP-dependent protein kinase: mechanism of enzyme activation
Proc. Natl. Acad. Sci. U.S.A. 75 (10), 4744-4748 (1978)
Joh,T.H., Park,D.H. and Reis,D.J.


 
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