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PRKACA cDNA ORF clone, Homo sapiens (human)

Summary

Gene Symbol PRKACA
Entrez Gene ID 5566
Full Name protein kinase, cAMP-dependent, catalytic, alpha
Synonyms PKACA, PPNAD4
General protein information
Preferred Names
cAMP-dependent protein kinase catalytic subunit alpha
Names
cAMP-dependent protein kinase catalytic subunit alpha
PKA C-alpha
protein kinase A catalytic subunit
cAMP-dependent protein kinase catalytic subunit alpha, isoform 1
NP_002721.1
NP_997401.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

19

19p13.1
Summary This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms. [provided by RefSeq, Jan 2015]. lac of sum
Disorder MIM:

601639


Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_207518 NP_997401 cAMP-dependent protein kinase catalytic subunit alpha isoform Calpha2
NM_001304349 NP_001291278 cAMP-dependent protein kinase catalytic subunit alpha isoform 3
NM_002730 NP_002721 cAMP-dependent protein kinase catalytic subunit alpha isoform Calpha1

Pathways from BioSystems


Homologies


Homo sapiens (human) PRKACA NP_002721.1
Pan troglodytes (chimpanzee) PRKACA XP_001171437.1
Canis lupus familiaris (dog) PRKACA NP_001003032.1
Bos taurus (cattle) PRKACA NP_777009.1
Mus musculus (house mouse) Prkaca NP_032880.1
Rattus norvegicus (Norway rat) Prkaca NP_001094392.1
Danio rerio (zebrafish) prkacab NP_001003470.1
Xenopus (Silurana) tropicalis (western clawed frog) prkaca NP_001016403.1

Gene Ontology


Bibliography

Related articles in PubMed

  1. Interlaboratory reproducibility of large-scale human protein-complex analysis by standardized AP-MS. Varjosalo M, et al. Nat Methods, 2013 Apr. PMID 23455922.
  2. Forskolin-inducible cAMP pathway negatively regulates T-cell proliferation by uncoupling the interleukin-2 receptor complex. Rodriguez G, et al. J Biol Chem, 2013 Mar 8. PMID 23341462.
  3. Regulation of SIK1 abundance and stability is critical for myogenesis. Stewart R, et al. Proc Natl Acad Sci U S A, 2013 Jan 2. PMID 23256157.
  4. New insights into the molecular mechanisms underlying sensitivity/resistance to the atypical retinoid ST1926 in acute myeloid leukaemia cells: the role of histone H2A.Z, cAMP-dependent protein kinase A and the proteasome. Fratelli M, et al. Eur J Cancer, 2013 Apr. PMID 23245330.
  5. Antagonism of VEGF-A-induced increase in vascular permeability by an integrin α3β1-Shp-1-cAMP/PKA pathway. Kim SH, et al. Blood, 2012 Dec 6. PMID 23074279.

See all (445) citations in PubMed

See citations in PubMed for homologs of this gene provided by HomoloGene

GeneRIFs: Gene References Into Functions What's a GeneRIF?

  1. Data indicate inhibition of cAMP-dependent protein kinase A with H89 partially reverted resistance of NB4.437r cells to ST1926.
    Title: New insights into the molecular mechanisms underlying sensitivity/resistance to the atypical retinoid ST1926 in acute myeloid leukaemia cells: the role of histone H2A.Z, cAMP-dependent protein kinase A and the proteasome.
  2. High CB1 and PKA expression is associated with low survival intracellular pathway activation and high iNOS activation in ischemic heart disease patients.
    Title: Different expression and function of the endocannabinoid system in human epicardial adipose tissue in relation to heart disease.
  3. Data suggest that cross-talk occurs between cAMP/PKA and the IL-2R beta/Jak3/Stat5b cascade in T-cells.
    Title: Forskolin-inducible cAMP pathway negatively regulates T-cell proliferation by uncoupling the interleukin-2 receptor complex.
  4. Glucagon-like peptide-1 inhibits angiotensin II-induced mesangial cell damage via protein kinase A
    Title: Glucagon-like peptide-1 inhibits angiotensin II-induced mesangial cell damage via protein kinase A.
  5. Data show that TIMP-2-mediated inhibition of vascular endothelial cell permeability involves an integrin alpha3beta1-Shp-1-cAMP/protein kinase A-dependent vascular endothelial cadherin cytoskeletal association.
    Title: Antagonism of VEGF-A-induced increase in vascular permeability by an integrin α3β1-Shp-1-cAMP/PKA pathway.
  6. In prostate cancer cells, S100A8 expression is stimulated by prostaglandin E2 via EP2 and EP4 receptors through activation of the protein kinase A signaling pathway and stimulation of CCAAT/enhancer-binding-protein-beta binding to the S100A8 promoter.
    Title: Prostaglandin E2 stimulates S100A8 expression by activating protein kinase A and CCAAT/enhancer-binding-protein-beta in prostate cancer cells.
  7. PKA-phosphodiesterase(PDE)4D3-A kinase anchor protein (AKAP)9 complex generates spatial compartmentalization of cyclic adenosine monophosphate (cAMP) signaling at the centrosome.
    Title: PKA and PDE4D3 anchoring to AKAP9 provides distinct regulation of cAMP signals at the centrosome.
  8. Activation of protein kinase A and exchange protein directly activated by cAMP promotes adipocyte differentiation of human mesenchymal stem cells
    Title: Activation of protein kinase A and exchange protein directly activated by cAMP promotes adipocyte differentiation of human mesenchymal stem cells.
  9. Data show that the cAMP/PKA/CREB signaling pathway initiates acidosis-induced V-ATPase trafficking in salivary ducts via regulation of Rab11b expression.
    Title: Acidosis-induced V-ATPase trafficking in salivary ducts is initiated by cAMP/PKA/CREB pathway via regulation of Rab11b expression.
  10. Data indicate that an increase in cAMP/PKA activity resulted in the dissociation of SIRT1 and DBC1 in an AMP-activated protein kinase (AMPK)-dependent manner.
    Title: Role of deleted in breast cancer 1 (DBC1) protein in SIRT1 deacetylase activation induced by protein kinase A and AMP-activated protein kinase.
Submit: New GeneRIF Correction See all (123)

The following PRKACA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRKACA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu17314 NM_207518 Homo sapiens protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $202.30
$289.00
OHu51697 NM_001304349 Homo sapiens protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu18669
 NM_002730 Homo sapiens protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $189.50-$265.30
$379.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu17314
Clone ID Related Accession (Same CDS sequence) NM_207518
Accession Version NM_207518.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1032bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cAMP-dependent protein kinase catalytic subunit alpha isoform Calpha2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK292284.1, BC108259.1 and BC039846.1. On Jan 17, 2015 this sequence version replaced gi:46909583. Transcript Variant: This variant (2) contains an alternate 5' terminal exon and initiates translation at an alternate start codon, compared to variant 1. It encodes Calpha2, PMID:21812984 (also known as C alpha-s, PMID: 10906071) which has a shorter and distinct N-terminus, compared to Calpha1. This variant is specifically expressed in spermatogenic cells and may be essential for male fertility. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292284.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA962337, SAMEA962343 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCTTCCA ACTCCAGCGA TGTGAAAGAA TTCTTAGCCA AAGCCAAAGA AGATTTTCTT 
AAAAAATGGG AAAGTCCCGC TCAGAACACA GCCCACTTGG ATCAGTTTGA ACGAATCAAG 
ACCCTCGGCA CGGGCTCCTT CGGGCGGGTG ATGCTGGTGA AACACAAGGA GACCGGGAAC 
CACTATGCCA TGAAGATCCT CGACAAACAG AAGGTGGTGA AACTGAAACA GATCGAACAC 
ACCCTGAATG AAAAGCGCAT CCTGCAAGCT GTCAACTTTC CGTTCCTCGT CAAACTCGAG 
TTCTCCTTCA AGGACAACTC AAACTTATAC ATGGTCATGG AGTACGTGCC CGGCGGGGAG 
ATGTTCTCAC ACCTACGGCG GATCGGAAGG TTCAGTGAGC CCCATGCCCG TTTCTACGCG 
GCCCAGATCG TCCTGACCTT TGAGTATCTG CACTCGCTGG ATCTCATCTA CAGGGACCTG 
AAGCCGGAGA ATCTGCTCAT TGACCAGCAG GGCTACATTC AGGTGACAGA CTTCGGTTTC 
GCCAAGCGCG TGAAGGGCCG CACTTGGACC TTGTGCGGCA CCCCTGAGTA CCTGGCCCCT 
GAGATTATCC TGAGCAAAGG CTACAACAAG GCCGTGGACT GGTGGGCCCT GGGGGTTCTT 
ATCTATGAAA TGGCCGCTGG CTACCCGCCC TTCTTCGCAG ACCAGCCCAT CCAGATCTAT 
GAGAAGATCG TCTCTGGGAA GGTGCGCTTC CCTTCCCACT TCAGCTCTGA CTTGAAGGAC 
CTGCTGCGGA ACCTCCTGCA GGTAGATCTC ACCAAGCGCT TTGGGAACCT CAAGAATGGG 
GTCAACGATA TCAAGAACCA CAAGTGGTTT GCCACAACTG ACTGGATTGC CATCTACCAG 
AGGAAGGTGG AAGCTCCCTT CATACCAAAG TTTAAAGGCC CTGGGGATAC GAGTAACTTT 
GACGACTATG AGGAAGAAGA AATCCGGGTC TCCATCAATG AGAAGTGTGG CAAGGAGTTT 
TCTGAGTTTT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_997401.1
CDS34..1065
Misc Feature(1)28..30(+)
Misc Feature(2)133..1002(+)
Misc Feature(3)139..903(+)
Misc Feature(4)157..993(+)
Misc Feature(5)160..1002(+)
Misc Feature(6)163..1002(+)
Misc Feature(7)169..1002(+)
Misc Feature(8)427..429(+)
Misc Feature(9)559..621(+)
Misc Feature(10)595..597(+)
Misc Feature(11)601..603(+)
Misc Feature(12)613..615(+)
Misc Feature(13)1024..1026(+)
Exon (1)1..55
Gene:PRKACA
Gene Synonym:
Exon (2)56..117
Gene:PRKACA
Gene Synonym:
Exon (3)118..246
Gene:PRKACA
Gene Synonym:
Exon (4)247..345
Gene:PRKACA
Gene Synonym:
Exon (5)346..428
Gene:PRKACA
Gene Synonym:
Exon (6)429..555
Gene:PRKACA
Gene Synonym:
Exon (7)556..651
Gene:PRKACA
Gene Synonym:
Exon (8)652..774
Gene:PRKACA
Gene Synonym:
Exon (9)775..939
Gene:PRKACA
Gene Synonym:
Exon (10)940..2489
Gene:PRKACA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_207518
Organism Homo sapiens (human)
Definition Homo sapiens protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_207518

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCTTCCA ACTCCAGCGA TGTGAAAGAA TTCTTAGCCA AAGCCAAAGA AGATTTTCTT 
AAAAAATGGG AAAGTCCCGC TCAGAACACA GCCCACTTGG ATCAGTTTGA ACGAATCAAG 
ACCCTCGGCA CGGGCTCCTT CGGGCGGGTG ATGCTGGTGA AACACAAGGA GACCGGGAAC 
CACTATGCCA TGAAGATCCT CGACAAACAG AAGGTGGTGA AACTGAAACA GATCGAACAC 
ACCCTGAATG AAAAGCGCAT CCTGCAAGCT GTCAACTTTC CGTTCCTCGT CAAACTCGAG 
TTCTCCTTCA AGGACAACTC AAACTTATAC ATGGTCATGG AGTACGTGCC CGGCGGGGAG 
ATGTTCTCAC ACCTACGGCG GATCGGAAGG TTCAGTGAGC CCCATGCCCG TTTCTACGCG 
GCCCAGATCG TCCTGACCTT TGAGTATCTG CACTCGCTGG ATCTCATCTA CAGGGACCTG 
AAGCCGGAGA ATCTGCTCAT TGACCAGCAG GGCTACATTC AGGTGACAGA CTTCGGTTTC 
GCCAAGCGCG TGAAGGGCCG CACTTGGACC TTGTGCGGCA CCCCTGAGTA CCTGGCCCCT 
GAGATTATCC TGAGCAAAGG CTACAACAAG GCCGTGGACT GGTGGGCCCT GGGGGTTCTT 
ATCTATGAAA TGGCCGCTGG CTACCCGCCC TTCTTCGCAG ACCAGCCCAT CCAGATCTAT 
GAGAAGATCG TCTCTGGGAA GGTGCGCTTC CCTTCCCACT TCAGCTCTGA CTTGAAGGAC 
CTGCTGCGGA ACCTCCTGCA GGTAGATCTC ACCAAGCGCT TTGGGAACCT CAAGAATGGG 
GTCAACGATA TCAAGAACCA CAAGTGGTTT GCCACAACTG ACTGGATTGC CATCTACCAG 
AGGAAGGTGG AAGCTCCCTT CATACCAAAG TTTAAAGGCC CTGGGGATAC GAGTAACTTT 
GACGACTATG AGGAAGAAGA AATCCGGGTC TCCATCAATG AGAAGTGTGG CAAGGAGTTT 
TCTGAGTTTT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu51697
Clone ID Related Accession (Same CDS sequence) NM_001304349
Accession Version NM_001304349.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1284bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cAMP-dependent protein kinase catalytic subunit alpha isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK131561.1, BC108259.1 and BC039846.1. On Jan 17, 2015 this sequence version replaced gi:530415007. Transcript Variant: This variant (3) contains an alternate 5' terminal exon and initiates translation at an alternate start codon, compared to variant 1. It encodes isoform 3 which has a longer and distinct N-terminus, compared to Calpha1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK131561.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2159607, SAMEA962337 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGTTCACCT TGGGATACCA TGGGGCCGAG GAGTGGGCAT CGGGCGAGAC TTCCCTTGAA 
CCAGCTCTGG AGCAATGCCC TTGCGATTGC CACGGAGAGG AACAGGGCCA GCTGGGGGCC 
AAGGTGACAG CTGAGCCCCC CAAGGGCACC TGCTACCCGA GCCCCCGAGG CCTCCATCCC 
AGGCCCGGCC TCCCCAGAGA AGGGGCCAGA GGAACAGCCA AATCCCAAGG CTATAAATAC 
CTCGGCTGGC GTCGGCCCTG GCCATGGAAT GTCGTGAAAG AATTCTTAGC CAAAGCCAAA 
GAAGATTTTC TTAAAAAATG GGAAAGTCCC GCTCAGAACA CAGCCCACTT GGATCAGTTT 
GAACGAATCA AGACCCTCGG CACGGGCTCC TTCGGGCGGG TGATGCTGGT GAAACACAAG 
GAGACCGGGA ACCACTATGC CATGAAGATC CTCGACAAAC AGAAGGTGGT GAAACTGAAA 
CAGATCGAAC ACACCCTGAA TGAAAAGCGC ATCCTGCAAG CTGTCAACTT TCCGTTCCTC 
GTCAAACTCG AGTTCTCCTT CAAGGACAAC TCAAACTTAT ACATGGTCAT GGAGTACGTG 
CCCGGCGGGG AGATGTTCTC ACACCTACGG CGGATCGGAA GGTTCAGTGA GCCCCATGCC 
CGTTTCTACG CGGCCCAGAT CGTCCTGACC TTTGAGTATC TGCACTCGCT GGATCTCATC 
TACAGGGACC TGAAGCCGGA GAATCTGCTC ATTGACCAGC AGGGCTACAT TCAGGTGACA 
GACTTCGGTT TCGCCAAGCG CGTGAAGGGC CGCACTTGGA CCTTGTGCGG CACCCCTGAG 
TACCTGGCCC CTGAGATTAT CCTGAGCAAA GGCTACAACA AGGCCGTGGA CTGGTGGGCC 
CTGGGGGTTC TTATCTATGA AATGGCCGCT GGCTACCCGC CCTTCTTCGC AGACCAGCCC 
ATCCAGATCT ATGAGAAGAT CGTCTCTGGG AAGGTGCGCT TCCCTTCCCA CTTCAGCTCT 
GACTTGAAGG ACCTGCTGCG GAACCTCCTG CAGGTAGATC TCACCAAGCG CTTTGGGAAC 
CTCAAGAATG GGGTCAACGA TATCAAGAAC CACAAGTGGT TTGCCACAAC TGACTGGATT 
GCCATCTACC AGAGGAAGGT GGAAGCTCCC TTCATACCAA AGTTTAAAGG CCCTGGGGAT 
ACGAGTAACT TTGACGACTA TGAGGAAGAA GAAATCCGGG TCTCCATCAA TGAGAAGTGT 
GGCAAGGAGT TTTCTGAGTT TTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001291278.1
CDS407..1690
Misc Feature(1)341..343(+)
Misc Feature(2)758..1627(+)
Misc Feature(3)764..1528(+)
Misc Feature(4)782..1618(+)
Misc Feature(5)785..1627(+)
Misc Feature(6)788..1627(+)
Misc Feature(7)794..1627(+)
Misc Feature(8)1184..1246(+)
Exon (1)1..680
Gene:PRKACA
Gene Synonym:
Exon (2)681..742
Gene:PRKACA
Gene Synonym:
Exon (3)743..871
Gene:PRKACA
Gene Synonym:
Exon (4)872..970
Gene:PRKACA
Gene Synonym:
Exon (5)971..1053
Gene:PRKACA
Gene Synonym:
Exon (6)1054..1180
Gene:PRKACA
Gene Synonym:
Exon (7)1181..1276
Gene:PRKACA
Gene Synonym:
Exon (8)1277..1399
Gene:PRKACA
Gene Synonym:
Exon (9)1400..1564
Gene:PRKACA
Gene Synonym:
Exon (10)1565..3114
Gene:PRKACA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001304349
Organism Homo sapiens (human)
Definition Homo sapiens protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001304349

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGTTCACCT TGGGATACCA TGGGGCCGAG GAGTGGGCAT CGGGCGAGAC TTCCCTTGAA 
CCAGCTCTGG AGCAATGCCC TTGCGATTGC CACGGAGAGG AACAGGGCCA GCTGGGGGCC 
AAGGTGACAG CTGAGCCCCC CAAGGGCACC TGCTACCCGA GCCCCCGAGG CCTCCATCCC 
AGGCCCGGCC TCCCCAGAGA AGGGGCCAGA GGAACAGCCA AATCCCAAGG CTATAAATAC 
CTCGGCTGGC GTCGGCCCTG GCCATGGAAT GTCGTGAAAG AATTCTTAGC CAAAGCCAAA 
GAAGATTTTC TTAAAAAATG GGAAAGTCCC GCTCAGAACA CAGCCCACTT GGATCAGTTT 
GAACGAATCA AGACCCTCGG CACGGGCTCC TTCGGGCGGG TGATGCTGGT GAAACACAAG 
GAGACCGGGA ACCACTATGC CATGAAGATC CTCGACAAAC AGAAGGTGGT GAAACTGAAA 
CAGATCGAAC ACACCCTGAA TGAAAAGCGC ATCCTGCAAG CTGTCAACTT TCCGTTCCTC 
GTCAAACTCG AGTTCTCCTT CAAGGACAAC TCAAACTTAT ACATGGTCAT GGAGTACGTG 
CCCGGCGGGG AGATGTTCTC ACACCTACGG CGGATCGGAA GGTTCAGTGA GCCCCATGCC 
CGTTTCTACG CGGCCCAGAT CGTCCTGACC TTTGAGTATC TGCACTCGCT GGATCTCATC 
TACAGGGACC TGAAGCCGGA GAATCTGCTC ATTGACCAGC AGGGCTACAT TCAGGTGACA 
GACTTCGGTT TCGCCAAGCG CGTGAAGGGC CGCACTTGGA CCTTGTGCGG CACCCCTGAG 
TACCTGGCCC CTGAGATTAT CCTGAGCAAA GGCTACAACA AGGCCGTGGA CTGGTGGGCC 
CTGGGGGTTC TTATCTATGA AATGGCCGCT GGCTACCCGC CCTTCTTCGC AGACCAGCCC 
ATCCAGATCT ATGAGAAGAT CGTCTCTGGG AAGGTGCGCT TCCCTTCCCA CTTCAGCTCT 
GACTTGAAGG ACCTGCTGCG GAACCTCCTG CAGGTAGATC TCACCAAGCG CTTTGGGAAC 
CTCAAGAATG GGGTCAACGA TATCAAGAAC CACAAGTGGT TTGCCACAAC TGACTGGATT 
GCCATCTACC AGAGGAAGGT GGAAGCTCCC TTCATACCAA AGTTTAAAGG CCCTGGGGAT 
ACGAGTAACT TTGACGACTA TGAGGAAGAA GAAATCCGGG TCTCCATCAA TGAGAAGTGT 
GGCAAGGAGT TTTCTGAGTT TTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18669
Clone ID Related Accession (Same CDS sequence) NM_002730
Accession Version NM_002730.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1056bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cAMP-dependent protein kinase catalytic subunit alpha isoform Calpha1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU543573.1 and BC039846.1. This sequence is a reference standard in the RefSeqGene project. On Apr 30, 2004 this sequence version replaced gi:31543437. Transcript Variant: This variant (1) encodes Calpha1 (PMID:21812984). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC108259.1, X07767.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA962346 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGGCAACG CCGCCGCCGC CAAGAAGGGC AGCGAGCAGG AGAGCGTGAA AGAATTCTTA 
GCCAAAGCCA AAGAAGATTT TCTTAAAAAA TGGGAAAGTC CCGCTCAGAA CACAGCCCAC 
TTGGATCAGT TTGAACGAAT CAAGACCCTC GGCACGGGCT CCTTCGGGCG GGTGATGCTG 
GTGAAACACA AGGAGACCGG GAACCACTAT GCCATGAAGA TCCTCGACAA ACAGAAGGTG 
GTGAAACTGA AACAGATCGA ACACACCCTG AATGAAAAGC GCATCCTGCA AGCTGTCAAC 
TTTCCGTTCC TCGTCAAACT CGAGTTCTCC TTCAAGGACA ACTCAAACTT ATACATGGTC 
ATGGAGTACG TGCCCGGCGG GGAGATGTTC TCACACCTAC GGCGGATCGG AAGGTTCAGT 
GAGCCCCATG CCCGTTTCTA CGCGGCCCAG ATCGTCCTGA CCTTTGAGTA TCTGCACTCG 
CTGGATCTCA TCTACAGGGA CCTGAAGCCG GAGAATCTGC TCATTGACCA GCAGGGCTAC 
ATTCAGGTGA CAGACTTCGG TTTCGCCAAG CGCGTGAAGG GCCGCACTTG GACCTTGTGC 
GGCACCCCTG AGTACCTGGC CCCTGAGATT ATCCTGAGCA AAGGCTACAA CAAGGCCGTG 
GACTGGTGGG CCCTGGGGGT TCTTATCTAT GAAATGGCCG CTGGCTACCC GCCCTTCTTC 
GCAGACCAGC CCATCCAGAT CTATGAGAAG ATCGTCTCTG GGAAGGTGCG CTTCCCTTCC 
CACTTCAGCT CTGACTTGAA GGACCTGCTG CGGAACCTCC TGCAGGTAGA TCTCACCAAG 
CGCTTTGGGA ACCTCAAGAA TGGGGTCAAC GATATCAAGA ACCACAAGTG GTTTGCCACA 
ACTGACTGGA TTGCCATCTA CCAGAGGAAG GTGGAAGCTC CCTTCATACC AAAGTTTAAA 
GGCCCTGGGG ATACGAGTAA CTTTGACGAC TATGAGGAAG AAGAAATCCG GGTCTCCATC 
AATGAGAAGT GTGGCAAGGA GTTTTCTGAG TTTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002721.1
CDS201..1256
Misc Feature(1)207..209(+)
Misc Feature(2)231..233(+)
Misc Feature(3)231..233(+)
Misc Feature(4)324..1193(+)
Misc Feature(5)330..1094(+)
Misc Feature(6)345..347(+)
Misc Feature(7)348..1184(+)
Misc Feature(8)351..1193(+)
Misc Feature(9)354..1193(+)
Misc Feature(10)360..1193(+)
Misc Feature(11)618..620(+)
Misc Feature(12)618..620(+)
Misc Feature(13)750..812(+)
Misc Feature(14)786..788(+)
Misc Feature(15)786..788(+)
Misc Feature(16)792..794(+)
Misc Feature(17)792..794(+)
Misc Feature(18)804..806(+)
Misc Feature(19)804..806(+)
Misc Feature(20)1191..1193(+)
Misc Feature(21)1215..1217(+)
Misc Feature(22)1215..1217(+)
Exon (1)1..246
Gene:PRKACA
Gene Synonym:
Exon (2)247..308
Gene:PRKACA
Gene Synonym:
Exon (3)309..437
Gene:PRKACA
Gene Synonym:
Exon (4)438..536
Gene:PRKACA
Gene Synonym:
Exon (5)537..619
Gene:PRKACA
Gene Synonym:
Exon (6)620..746
Gene:PRKACA
Gene Synonym:
Exon (7)747..842
Gene:PRKACA
Gene Synonym:
Exon (8)843..965
Gene:PRKACA
Gene Synonym:
Exon (9)966..1130
Gene:PRKACA
Gene Synonym:
Exon (10)1131..2680
Gene:PRKACA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_002730
Organism Homo sapiens (human)
Definition Homo sapiens protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_002730

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGGCAACG CCGCCGCCGC CAAGAAGGGC AGCGAGCAGG AGAGCGTGAA AGAATTCTTA 
GCCAAAGCCA AAGAAGATTT TCTTAAAAAA TGGGAAAGTC CCGCTCAGAA CACAGCCCAC 
TTGGATCAGT TTGAACGAAT CAAGACCCTC GGCACGGGCT CCTTCGGGCG GGTGATGCTG 
GTGAAACACA AGGAGACCGG GAACCACTAT GCCATGAAGA TCCTCGACAA ACAGAAGGTG 
GTGAAACTGA AACAGATCGA ACACACCCTG AATGAAAAGC GCATCCTGCA AGCTGTCAAC 
TTTCCGTTCC TCGTCAAACT CGAGTTCTCC TTCAAGGACA ACTCAAACTT ATACATGGTC 
ATGGAGTACG TGCCCGGCGG GGAGATGTTC TCACACCTAC GGCGGATCGG AAGGTTCAGT 
GAGCCCCATG CCCGTTTCTA CGCGGCCCAG ATCGTCCTGA CCTTTGAGTA TCTGCACTCG 
CTGGATCTCA TCTACAGGGA CCTGAAGCCG GAGAATCTGC TCATTGACCA GCAGGGCTAC 
ATTCAGGTGA CAGACTTCGG TTTCGCCAAG CGCGTGAAGG GCCGCACTTG GACCTTGTGC 
GGCACCCCTG AGTACCTGGC CCCTGAGATT ATCCTGAGCA AAGGCTACAA CAAGGCCGTG 
GACTGGTGGG CCCTGGGGGT TCTTATCTAT GAAATGGCCG CTGGCTACCC GCCCTTCTTC 
GCAGACCAGC CCATCCAGAT CTATGAGAAG ATCGTCTCTG GGAAGGTGCG CTTCCCTTCC 
CACTTCAGCT CTGACTTGAA GGACCTGCTG CGGAACCTCC TGCAGGTAGA TCTCACCAAG 
CGCTTTGGGA ACCTCAAGAA TGGGGTCAAC GATATCAAGA ACCACAAGTG GTTTGCCACA 
ACTGACTGGA TTGCCATCTA CCAGAGGAAG GTGGAAGCTC CCTTCATACC AAAGTTTAAA 
GGCCCTGGGG ATACGAGTAA CTTTGACGAC TATGAGGAAG AAGAAATCCG GGTCTCCATC 
AATGAGAAGT GTGGCAAGGA GTTTTCTGAG TTTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Recurrent activating mutation in PRKACA in cortisol-producing adrenal tumors
Nat. Genet. 46 (6), 613-617 (2014)
Goh G, Scholl UI, Healy JM, Choi M, Prasad ML, Nelson-Williams C, Kunstman JW, Korah R, Suttorp AC, Dietrich D, Haase M, Willenberg HS, Stalberg P, Hellman P, Akerstrom G, Bjorklund P, Carling T and Lifton RP.

book

Recurrent somatic mutations underlie corticotropin-independent Cushing's syndrome
Science 344 (6186), 917-920 (2014)
Sato Y, Maekawa S, Ishii R, Sanada M, Morikawa T, Shiraishi Y, Yoshida K, Nagata Y, Sato-Otsubo A, Yoshizato T, Suzuki H, Shiozawa Y, Kataoka K, Kon A, Aoki K, Chiba K, Tanaka H, Kume H, Miyano S, Fukayama M, Nureki O, Homma Y and Ogawa S.

book

Activating hotspot L205R mutation in PRKACA and adrenal Cushing's syndrome
Science 344 (6186), 913-917 (2014)
Cao Y, He M, Gao Z, Peng Y, Li Y, Li L, Zhou W, Li X, Zhong X, Lei Y, Su T, Wang H, Jiang Y, Yang L, Wei W, Yang X, Jiang X, Liu L, He J, Ye J, Wei Q, Li Y, Wang W, Wang J and Ning G.

book

Constitutive activation of PKA catalytic subunit in adrenal Cushing's syndrome
N. Engl. J. Med. 370 (11), 1019-1028 (2014)
Beuschlein F, Fassnacht M, Assie G, Calebiro D, Stratakis CA, Osswald A, Ronchi CL, Wieland T, Sbiera S, Faucz FR, Schaak K, Schmittfull A, Schwarzmayr T, Barreau O, Vezzosi D, Rizk-Rabin M, Zabel U, Szarek E, Salpea P, Forlino A, Vetro A, Zuffardi O, Kisker C, Diener S, Meitinger T, Lohse MJ, Reincke M, Bertherat J, Strom TM and Allolio B.

book

The unique catalytic subunit of sperm cAMP-dependent protein kinase is the product of an alternative Calpha mRNA expressed specifically in spermatogenic cells
Mol. Biol. Cell 11 (9), 3031-3044 (2000)
Agustin JT, Wilkerson CG and Witman GB.

book

A novel isoform of human cyclic 3',5'-adenosine monophosphate-dependent protein kinase, c alpha-s, localizes to sperm midpiece
Biol. Reprod. 63 (2), 607-611 (2000)
Reinton N, Orstavik S, Haugen TB, Jahnsen T, Tasken K and Skalhegg BS.

book

Expression of a nonmyristylated variant of the catalytic subunit of protein kinase A during male germ-cell development
Proc. Natl. Acad. Sci. U.S.A. 97 (12), 6433-6438 (2000)
Desseyn JL, Burton KA and McKnight GS.

book

A cDNA clone encoding human cAMP-dependent protein kinase catalytic subunit C alpha
Nucleic Acids Res. 16 (16), 8189-8190 (1988)
Maldonado F and Hanks SK.

book

Sequence of two phosphorylated sites in the catalytic subunit of bovine cardiac muscle adenosine 3':5'-monophosphate-dependent protein kinase
J. Biol. Chem. 254 (14), 6211-6214 (1979)
Shoji,S., Titani,K., Demaille,J.G. and Fischer,E.H.

book

Direct phosphorylation of brain tyrosine hydroxylase by cyclic AMP-dependent protein kinase: mechanism of enzyme activation
Proc. Natl. Acad. Sci. U.S.A. 75 (10), 4744-4748 (1978)
Joh,T.H., Park,D.H. and Reis,D.J.

 
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