CHFR cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHFR
Entrez Gene ID	55743
Full Name	checkpoint with forkhead and ring finger domains
Synonyms	RNF116,RNF196
General protein information	Preferred Names checkpoint with forkhead and ring finger domains Names E3 ubiquitin-protein ligase CHFR RING finger protein 196 RING-type E3 ubiquitin transferase CHFR checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q24.33
Summary	This gene encodes an E3 ubiquitin-protein ligase required for the maintenance of the antephase checkpoint that regulates cell cycle entry into mitosis and, therefore, may play a key role in cell cycle progression and tumorigenesis. The encoded protein has an N-terminal forkhead-associated domain, a central RING-finger domain, and a cysteine-rich C-terminal region. Alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq, Mar 2014].
Disorder MIM:	605209

mRNA and Protein(s)

mRNA	Protein	Name
NM_018223.2	NP_060693.2	E3 ubiquitin-protein ligase CHFR isoform 4
NM_001161344.1	NP_001154816.1	E3 ubiquitin-protein ligase CHFR isoform 1
NM_001161345.1	NP_001154817.1	E3 ubiquitin-protein ligase CHFR isoform 2
NM_001161346.2	NP_001154818.1	E3 ubiquitin-protein ligase CHFR isoform 3
NM_001161346.1	NP_001154818.1	E3 ubiquitin-protein ligase CHFR isoform 3
NM_001161347.1	NP_001154819.1	E3 ubiquitin-protein ligase CHFR isoform 5

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Chfr	NP_001009258.1
Xenopus tropicalis (tropical clawed frog)	chfr	NP_989378.1
	CHFR	XP_001083796.2
Oryza sativa (rice)	Os11g0175500	NP_001065879.2
Homo sapiens (human)	CHFR	NP_001154816.1
Pan troglodytes (chimpanzee)	CHFR	XP_003314094.2
Bos taurus (cattle)	CHFR	NP_001192403.1
Danio rerio (zebrafish)	chfr	NP_001093485.1
Canis lupus familiaris (dog)	CHFR	XP_534631.3
Arabidopsis thaliana (thale cress)	AT1G47570	NP_001077687.1
Mus musculus (house mouse)	Chfr	NP_766305.2
Gallus gallus (chicken)	CHFR	XP_004945549.1

Gene Ontology
Bibliography

Related articles in PubMed

Methylation-independent CHFR expression is a potential biomarker affecting prognosis in acute myeloid leukemia.
Zhou JD, Zhang TJ, Li XX, Ma JC, Guo H, Wen XM, Yao DM, Zhang W, Lin J, Qian J
Journal of cellular physiology(2017 Nov)

Sustained expression of miR-26a promotes chromosomal instability and tumorigenesis through regulation of CHFR.
Castellano L, Dabrowska A, Pellegrino L, Ottaviani S, Cathcart P, Frampton AE, Krell J, Stebbing J
Nucleic acids research45(8)4401-4412(2017 May)

CHFR negatively regulates SIRT1 activity upon oxidative stress.
Kim M, Kwon YE, Song JO, Bae SJ, Seol JH
Scientific reports637578(2016 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Re-expression of CHFR in miR-26a overexpressing cells partially rescues normal mitosis and impairs the tumorigenesis exerted by miR-26a.
Title: Sustained expression of miR-26a promotes chromosomal instability and tumorigenesis through regulation of CHFR.

Data show that hypermethylation of the CHFR promoter, frequent in acute myeloid leukemia, is associated with adverse outcome, and can thus be used for risk stratification.
Title: CHFR hypermethylation, a frequent event in acute myeloid leukemia, is independently associated with an adverse outcome.

Small molecule inhibition of CHFR-PARP1 interaction is a novel potential therapeutic approach to increase the efficacy of taxane-based chemotherapy in cancer.
Title: Small molecule inhibition of the CHFR-PARP1 interaction as novel approach to overcome intrinsic taxane resistance in cancer.

Aberrant methylation of CHFR may be associated with the pathogenesis, progression for B-cell non-Hodgkin lymphoma(B-NHL), which might be a novel molecular marker as prognosis and treatment for B-NHL.
Title: Aberrant expression of the CHFR prophase checkpoint gene in human B-cell non-Hodgkin lymphoma.

CHFR methylation has a role in methylation of DNA damage repair and apoptotic pathway genes in non-small cell lung cancer
Title: CHFR methylation strongly correlates with methylation of DNA damage repair and apoptotic pathway genes in non-small cell lung cancer.

The following CHFR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHFR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu15061	NM_001161344.1 Latest version!	Homo sapiens checkpoint with forkhead and ring finger domains (CHFR), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu22042	NM_018223.2 Latest version!	Homo sapiens checkpoint with forkhead and ring finger domains (CHFR), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu15152	NM_001161346.1 Latest version!	Homo sapiens checkpoint with forkhead and ring finger domains (CHFR), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu15331	NM_001161345.1 Latest version!	Homo sapiens checkpoint with forkhead and ring finger domains (CHFR), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu15351	NM_001161347.1 Latest version!	Homo sapiens checkpoint with forkhead and ring finger domains (CHFR), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu109451	NM_001161346.2 Latest version!	Homo sapiens checkpoint with forkhead and ring finger domains (CHFR), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHFR ( NM_001161344.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001154816.1 Homo sapiens checkpoint with forkhead and ring finger domains (CHFR), transcript variant 1, mRNA.

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CloneID	OHu15061
Clone ID Related Accession (Same CDS sequence)	NM_001161344.1
Accession Version	NM_001161344.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase CHFR isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP313700.1, AF170724.1, AC127070.10 and BC012072.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.193923.1, AF170724.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCGGC CCGAGGAAGG CAAGCAGTCG CCGCCGCCGC AGCCCTGGGG ACGGCTCCTG 
CGTCTGGGCG CGGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAGCGGGA GTGGACCATC 
GGGCGGAGAC GAGGTTGCGA CCTTTCCTTC CCCAGCAATA AACTGGTCTC TGGAGATCAC 
TGTAGAATTG TAGTGGATGA AAAATCAGGT CAGGTGACAC TGGAAGATAC CAGCACCAGT 
GGAACAGTGA TTAACAAGCT GAAGGTTGTT AAGAAGCAGA CATGCCCTTT ACAGACTGGG 
GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAACCGGAAC ACAACGTGGC ATACCTCTAT 
GAATCTTTAA GTGAAAAGCA AGGCATGACA CAAGAATCCT TTGAAGCTAA CAAGGAAAAT 
GTGTTCCATG GGACCAAAGA TACCTCAGGT GCAGGTGCAG GGCGAGGGGC CGATCCCCGG 
GTCCCTCCGT CGTCGCCCGC CACTCAGGTG TGCTTTGAGG AACCACAGCC ATCAACATCG 
ACGTCAGACC TCTTCCCCAC AGCCTCGGCC TCTTCCACGG AGCCTTCTCC TGCAGGGCGA 
GAGCGTTCCT CCAGTTGTGG GTCTGGGGGT GGTGGCATCT CCCCTAAAGG AAGTGGTCCC 
TCTGTGGCAA GTGATGAAGT CTCCAGCTTT GCCTCAGCTC TCCCAGACAG AAAGACTGCG 
TCCTTTTCGT CGTTGGAACC CCAGGATCAG GAGGATTTGG AGCCCGTGAA GAAGAAAATG 
AGAGGAGATG GGGACCTTGA CCTGAACGGG CAGTTGTTGG TCGCACAACC GCGTAGAAAT 
GCCCAAACCG TCCACGAGGA CGTCAGAGCA GCGGCTGGGA AGCCAGACAA GATGGAGGAG 
ACGCTGACAT GCATCATCTG CCAGGACCTG CTGCACGACT GCGTGAGTTT GCAGCCCTGC 
ATGCACACGT TCTGCGCGGC TTGCTACTCG GGCTGGATGG AGCGCTCGTC CCTGTGTCCT 
ACCTGCCGCT GTCCCGTGGA GCGGATCTGT AAAAACCACA TCCTCAACAA CCTCGTGGAA 
GCATACCTCA TCCAGCATCC AGACAAGAGT CGCAGTGAAG AAGATGTGCA AAGTATGGAT 
GCCAGGAATA AAATCACTCA AGACATGCTG CAGCCCAAAG TCAGGCGGTC TTTTTCTGAT 
GAAGAAGGGA GTTCAGAGGA CCTGCTGGAG CTGTCAGACG TTGACAGTGA GTCCTCAGAC 
ATTAGCCAGC CATACGTCGT GTGCCGGCAG TGTCCTGAGT ACAGAAGGCA GGCGGCGCAG 
CCTCCCCACT GCCCAGCACC CGAGGGCGAG CCAGGAGCCC CACAGGCCCT GGGGGATGCA 
CCCTCCACGT CCGTCAGCCT GACGACAGCA GTCCAGGATT ACGTGTGCCC TCTGCAAGGA 
AGCCACGCCC TGTGCACCTG CTGCTTCCAG CCCATGCCCG ACCGGAGAGC GGAGCGCGAG 
CAGGACCCGC GTGTCGCCCC TCAGCAGTGT GCGGTCTGCC TGCAGCCTTT CTGCCACCTG 
TACTGGGGCT GCACCCGGAC CGGCTGCTAC GGCTGCCTGG CCCCGTTTTG TGAGCTCAAC 
CTGGGTGACA AGTGTCTGGA CGGCGTGCTG AACAACAACA GCTACGAGTC AGACATCCTG 
AAGAATTACC TGGCAACCAG AGGTTTGACA TGGAAAAACA TGTTGACCGA GAGCCTCGTG 
GCTCTCCAGC GGGGAGTGTT TCTGCTGTCT GATTACAGAG TCACGGGAGA CACCGTTCTG 
TGTTACTGCT GTGGCCTGCG CAGCTTCCGT GAGCTGACCT ATCAGTATCG GCAGAACATT 
CCTGCTTCCG AGTTGCCAGT GGCCGTAACA TCCCGTCCTG ACTGCTACTG GGGCCGTAAC 
TGCCGCACTC AGGTGAAAGC TCACCACGCC ATGAAATTCA ATCATATCTG TGAACAGACA 
AGGTTCAAAA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001154816.1
CDS	85..2079
Translation	MERPEEGKQSPPPQPWGRLLRLGAEEGEPHVLLRKREWTIGRRRGCDLSFPSNKLVSGDHCRIVVDEKSGQVTLEDTSTSGTVINKLKVVKKQTCPLQTGDVIYLVYRKNEPEHNVAYLYESLSEKQGMTQESFEANKENVFHGTKDTSGAGAGRGADPRVPPSSPATQVCFEEPQPSTSTSDLFPTASASSTEPSPAGRERSSSCGSGGGGISPKGSGPSVASDEVSSFASALPDRKTASFSSLEPQDQEDLEPVKKKMRGDGDLDLNGQLLVAQPRRNAQTVHEDVRAAAGKPDKMEETLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSSLCPTCRCPVERICKNHILNNLVEAYLIQHPDKSRSEEDVQSMDARNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESSDISQPYVVCRQCPEYRRQAAQPPHCPAPEGEPGAPQALGDAPSTSVSLTTAVQDYVCPLQGSHALCTCCFQPMPDRRAEREQDPRVAPQQCAVCLQPFCHLYWGCTRTGCYGCLAPFCELNLGDKCLDGVLNNNSYESDILKNYLATRGLTWKNMLTESLVALQRGVFLLSDYRVTGDTVLCYCCGLRSFRELTYQYRQNIPASELPVAVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN

Target ORF information:

RefSeq Version	NM_001161344.1
Organism	Homo sapiens(human)
Definition	Homo sapiens checkpoint with forkhead and ring finger domains (CHFR), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001161344.1

ORF Insert Sequence:

ATGGAGCGGC CCGAGGAAGG CAAGCAGTCG CCGCCGCCGC AGCCCTGGGG ACGGCTCCTG 
CGTCTGGGCG CGGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAGCGGGA GTGGACCATC 
GGGCGGAGAC GAGGTTGCGA CCTTTCCTTC CCCAGCAATA AACTGGTCTC TGGAGATCAC 
TGTAGAATTG TAGTGGATGA AAAATCAGGT CAGGTGACAC TGGAAGATAC CAGCACCAGT 
GGAACAGTGA TTAACAAGCT GAAGGTTGTT AAGAAGCAGA CATGCCCTTT ACAGACTGGG 
GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAACCGGAAC ACAACGTGGC ATACCTCTAT 
GAATCTTTAA GTGAAAAGCA AGGCATGACA CAAGAATCCT TTGAAGCTAA CAAGGAAAAT 
GTGTTCCATG GGACCAAAGA TACCTCAGGT GCAGGTGCAG GGCGAGGGGC CGATCCCCGG 
GTCCCTCCGT CGTCGCCCGC CACTCAGGTG TGCTTTGAGG AACCACAGCC ATCAACATCG 
ACGTCAGACC TCTTCCCCAC AGCCTCGGCC TCTTCCACGG AGCCTTCTCC TGCAGGGCGA 
GAGCGTTCCT CCAGTTGTGG GTCTGGGGGT GGTGGCATCT CCCCTAAAGG AAGTGGTCCC 
TCTGTGGCAA GTGATGAAGT CTCCAGCTTT GCCTCAGCTC TCCCAGACAG AAAGACTGCG 
TCCTTTTCGT CGTTGGAACC CCAGGATCAG GAGGATTTGG AGCCCGTGAA GAAGAAAATG 
AGAGGAGATG GGGACCTTGA CCTGAACGGG CAGTTGTTGG TCGCACAACC GCGTAGAAAT 
GCCCAAACCG TCCACGAGGA CGTCAGAGCA GCGGCTGGGA AGCCAGACAA GATGGAGGAG 
ACGCTGACAT GCATCATCTG CCAGGACCTG CTGCACGACT GCGTGAGTTT GCAGCCCTGC 
ATGCACACGT TCTGCGCGGC TTGCTACTCG GGCTGGATGG AGCGCTCGTC CCTGTGTCCT 
ACCTGCCGCT GTCCCGTGGA GCGGATCTGT AAAAACCACA TCCTCAACAA CCTCGTGGAA 
GCATACCTCA TCCAGCATCC AGACAAGAGT CGCAGTGAAG AAGATGTGCA AAGTATGGAT 
GCCAGGAATA AAATCACTCA AGACATGCTG CAGCCCAAAG TCAGGCGGTC TTTTTCTGAT 
GAAGAAGGGA GTTCAGAGGA CCTGCTGGAG CTGTCAGACG TTGACAGTGA GTCCTCAGAC 
ATTAGCCAGC CATACGTCGT GTGCCGGCAG TGTCCTGAGT ACAGAAGGCA GGCGGCGCAG 
CCTCCCCACT GCCCAGCACC CGAGGGCGAG CCAGGAGCCC CACAGGCCCT GGGGGATGCA 
CCCTCCACGT CCGTCAGCCT GACGACAGCA GTCCAGGATT ACGTGTGCCC TCTGCAAGGA 
AGCCACGCCC TGTGCACCTG CTGCTTCCAG CCCATGCCCG ACCGGAGAGC GGAGCGCGAG 
CAGGACCCGC GTGTCGCCCC TCAGCAGTGT GCGGTCTGCC TGCAGCCTTT CTGCCACCTG 
TACTGGGGCT GCACCCGGAC CGGCTGCTAC GGCTGCCTGG CCCCGTTTTG TGAGCTCAAC 
CTGGGTGACA AGTGTCTGGA CGGCGTGCTG AACAACAACA GCTACGAGTC AGACATCCTG 
AAGAATTACC TGGCAACCAG AGGTTTGACA TGGAAAAACA TGTTGACCGA GAGCCTCGTG 
GCTCTCCAGC GGGGAGTGTT TCTGCTGTCT GATTACAGAG TCACGGGAGA CACCGTTCTG 
TGTTACTGCT GTGGCCTGCG CAGCTTCCGT GAGCTGACCT ATCAGTATCG GCAGAACATT 
CCTGCTTCCG AGTTGCCAGT GGCCGTAACA TCCCGTCCTG ACTGCTACTG GGGCCGTAAC 
TGCCGCACTC AGGTGAAAGC TCACCACGCC ATGAAATTCA ATCATATCTG TGAACAGACA 
AGGTTCAAAA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHFR ( NM_018223.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_060693.2 Homo sapiens checkpoint with forkhead and ring finger domains (CHFR), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu22042
Clone ID Related Accession (Same CDS sequence)	NM_018223.2
Accession Version	NM_018223.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-01
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase CHFR isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP313700.1, AK001658.1, AK027687.1, AC127070.10 and BC012072.1. This sequence is a reference standard in the RefSeqGene project. On Jun 6, 2009 this sequence version replaced NM_018223.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK001658.1, SRR1660809.224931.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCGGC CCGAGGAAGG CAAGCAGTCG CCGCCGCCGC AGCCCTGGGG ACGGCTCCTG 
CGTCTGGGCG CGGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAGCGGGA GTGGACCATC 
GGGCGGAGAC GAGGTTGCGA CCTTTCCTTC CCCAGCAATA AACTGGTCTC TGGAGATCAC 
TGTAGAATTG TAGTGGATGA AAAATCAGGT CAGGTGACAC TGGAAGATAC CAGCACCAGT 
GGAACAGTGA TTAACAAGCT GAAGGTTGTT AAGAAGCAGA CATGCCCTTT ACAGACTGGG 
GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAACCGGAAC ACAACGTGGC ATACCTCTAT 
GAATCTTTAA GTGAAAAGCA AGGCATGACA CAAGAATCCT TTGAGATGGT GCCTTGCTGT 
GTTGCCCAGG CTGGTCTAAA ACTCCTGGGA TCAAGTGATC CTCCCACCTT GGCCTCCCAA 
AGTATTGTGA TTACAGGGTC TGGGGGTGGT GGCATCTCCC CTAAAGGAAG TGGTCCCTCT 
GTGGCAAGTG ATGAAGTCTC CAGCTTTGCC TCAGCTCTCC CAGACAGAAA GACTGCGTCC 
TTTTCGTCGT TGGAACCCCA GGATCAGGAG GATTTGGAGC CCGTGAAGAA GAAAATGAGA 
GGAGATGGGG ACCTTGACCT GAACGGGCAG TTGTTGGTCG CACAACCGCG TAGAAATGCC 
CAAACCGTCC ACGAGGACGT CAGAGCAGCG GCTGGGAAGC CAGACAAGAT GGAGGAGACG 
CTGACATGCA TCATCTGCCA GGACCTGCTG CACGACTGCG TGAGTTTGCA GCCCTGCATG 
CACACGTTCT GCGCGGCTTG CTACTCGGGC TGGATGGAGC GCTCGTCCCT GTGTCCTACC 
TGCCGCTGTC CCGTGGAGCG GATCTGTAAA AACCACATCC TCAACAACCT CGTGGAAGCA 
TACCTCATCC AGCATCCAGA CAAGAGTCGC AGTGAAGAAG ATGTGCAAAG TATGGATGCC 
AGGAATAAAA TCACTCAAGA CATGCTGCAG CCCAAAGTCA GGCGGTCTTT TTCTGATGAA 
GAAGGGAGTT CAGAGGACCT GCTGGAGCTG TCAGACGTTG ACAGTGAGTC CTCAGACATT 
AGCCAGCCAT ACGTCGTGTG CCGGCAGTGT CCTGAGTACA GAAGGCAGGC GGCGCAGCCT 
CCCCACTGCC CAGCACCCGA GGGCGAGCCA GGAGCCCCAC AGGCCCTGGG GGATGCACCC 
TCCACGTCCG TCAGCCTGAC GACAGCAGTC CAGGATTACG TGTGCCCTCT GCAAGGAAGC 
CACGCCCTGT GCACCTGCTG CTTCCAGCCC ATGCCCGACC GGAGAGCGGA GCGCGAGCAG 
GACCCGCGTG TCGCCCCTCA GCAGTGTGCG GTCTGCCTGC AGCCTTTCTG CCACCTGTAC 
TGGGGCTGCA CCCGGACCGG CTGCTACGGC TGCCTGGCCC CGTTTTGTGA GCTCAACCTG 
GGTGACAAGT GTCTGGACGG CGTGCTGAAC AACAACAGCT ACGAGTCAGA CATCCTGAAG 
AATTACCTGG CAACCAGAGG TTTGACATGG AAAAACATGT TGACCGAGAG CCTCGTGGCT 
CTCCAGCGGG GAGTGTTTCT GCTGTCTGAT TACAGAGTCA CGGGAGACAC CGTTCTGTGT 
TACTGCTGTG GCCTGCGCAG CTTCCGTGAG CTGACCTATC AGTATCGGCA GAACATTCCT 
GCTTCCGAGT TGCCAGTGGC CGTAACATCC CGTCCTGACT GCTACTGGGG CCGTAACTGC 
CGCACTCAGG TGAAAGCTCA CCACGCCATG AAATTCAATC ATATCTGTGA ACAGACAAGG 
TTCAAAAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060693.2
CDS	85..1956
Translation	MERPEEGKQSPPPQPWGRLLRLGAEEGEPHVLLRKREWTIGRRRGCDLSFPSNKLVSGDHCRIVVDEKSGQVTLEDTSTSGTVINKLKVVKKQTCPLQTGDVIYLVYRKNEPEHNVAYLYESLSEKQGMTQESFEMVPCCVAQAGLKLLGSSDPPTLASQSIVITGSGGGGISPKGSGPSVASDEVSSFASALPDRKTASFSSLEPQDQEDLEPVKKKMRGDGDLDLNGQLLVAQPRRNAQTVHEDVRAAAGKPDKMEETLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSSLCPTCRCPVERICKNHILNNLVEAYLIQHPDKSRSEEDVQSMDARNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESSDISQPYVVCRQCPEYRRQAAQPPHCPAPEGEPGAPQALGDAPSTSVSLTTAVQDYVCPLQGSHALCTCCFQPMPDRRAEREQDPRVAPQQCAVCLQPFCHLYWGCTRTGCYGCLAPFCELNLGDKCLDGVLNNNSYESDILKNYLATRGLTWKNMLTESLVALQRGVFLLSDYRVTGDTVLCYCCGLRSFRELTYQYRQNIPASELPVAVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN

Target ORF information:

RefSeq Version	NM_018223.2
Organism	Homo sapiens(human)
Definition	Homo sapiens checkpoint with forkhead and ring finger domains (CHFR), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018223.2

ORF Insert Sequence:

ATGGAGCGGC CCGAGGAAGG CAAGCAGTCG CCGCCGCCGC AGCCCTGGGG ACGGCTCCTG 
CGTCTGGGCG CGGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAGCGGGA GTGGACCATC 
GGGCGGAGAC GAGGTTGCGA CCTTTCCTTC CCCAGCAATA AACTGGTCTC TGGAGATCAC 
TGTAGAATTG TAGTGGATGA AAAATCAGGT CAGGTGACAC TGGAAGATAC CAGCACCAGT 
GGAACAGTGA TTAACAAGCT GAAGGTTGTT AAGAAGCAGA CATGCCCTTT ACAGACTGGG 
GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAACCGGAAC ACAACGTGGC ATACCTCTAT 
GAATCTTTAA GTGAAAAGCA AGGCATGACA CAAGAATCCT TTGAGATGGT GCCTTGCTGT 
GTTGCCCAGG CTGGTCTAAA ACTCCTGGGA TCAAGTGATC CTCCCACCTT GGCCTCCCAA 
AGTATTGTGA TTACAGGGTC TGGGGGTGGT GGCATCTCCC CTAAAGGAAG TGGTCCCTCT 
GTGGCAAGTG ATGAAGTCTC CAGCTTTGCC TCAGCTCTCC CAGACAGAAA GACTGCGTCC 
TTTTCGTCGT TGGAACCCCA GGATCAGGAG GATTTGGAGC CCGTGAAGAA GAAAATGAGA 
GGAGATGGGG ACCTTGACCT GAACGGGCAG TTGTTGGTCG CACAACCGCG TAGAAATGCC 
CAAACCGTCC ACGAGGACGT CAGAGCAGCG GCTGGGAAGC CAGACAAGAT GGAGGAGACG 
CTGACATGCA TCATCTGCCA GGACCTGCTG CACGACTGCG TGAGTTTGCA GCCCTGCATG 
CACACGTTCT GCGCGGCTTG CTACTCGGGC TGGATGGAGC GCTCGTCCCT GTGTCCTACC 
TGCCGCTGTC CCGTGGAGCG GATCTGTAAA AACCACATCC TCAACAACCT CGTGGAAGCA 
TACCTCATCC AGCATCCAGA CAAGAGTCGC AGTGAAGAAG ATGTGCAAAG TATGGATGCC 
AGGAATAAAA TCACTCAAGA CATGCTGCAG CCCAAAGTCA GGCGGTCTTT TTCTGATGAA 
GAAGGGAGTT CAGAGGACCT GCTGGAGCTG TCAGACGTTG ACAGTGAGTC CTCAGACATT 
AGCCAGCCAT ACGTCGTGTG CCGGCAGTGT CCTGAGTACA GAAGGCAGGC GGCGCAGCCT 
CCCCACTGCC CAGCACCCGA GGGCGAGCCA GGAGCCCCAC AGGCCCTGGG GGATGCACCC 
TCCACGTCCG TCAGCCTGAC GACAGCAGTC CAGGATTACG TGTGCCCTCT GCAAGGAAGC 
CACGCCCTGT GCACCTGCTG CTTCCAGCCC ATGCCCGACC GGAGAGCGGA GCGCGAGCAG 
GACCCGCGTG TCGCCCCTCA GCAGTGTGCG GTCTGCCTGC AGCCTTTCTG CCACCTGTAC 
TGGGGCTGCA CCCGGACCGG CTGCTACGGC TGCCTGGCCC CGTTTTGTGA GCTCAACCTG 
GGTGACAAGT GTCTGGACGG CGTGCTGAAC AACAACAGCT ACGAGTCAGA CATCCTGAAG 
AATTACCTGG CAACCAGAGG TTTGACATGG AAAAACATGT TGACCGAGAG CCTCGTGGCT 
CTCCAGCGGG GAGTGTTTCT GCTGTCTGAT TACAGAGTCA CGGGAGACAC CGTTCTGTGT 
TACTGCTGTG GCCTGCGCAG CTTCCGTGAG CTGACCTATC AGTATCGGCA GAACATTCCT 
GCTTCCGAGT TGCCAGTGGC CGTAACATCC CGTCCTGACT GCTACTGGGG CCGTAACTGC 
CGCACTCAGG TGAAAGCTCA CCACGCCATG AAATTCAATC ATATCTGTGA ACAGACAAGG 
TTCAAAAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHFR ( NM_001161346.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001154818.1 Homo sapiens checkpoint with forkhead and ring finger domains (CHFR), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu15152
Clone ID Related Accession (Same CDS sequence)	NM_001161346.1
Accession Version	NM_001161346.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase CHFR isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP313700.1, AK027687.1, AC127070.10 and BC012072.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. This results in a shorter protein (isoform 3) compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.125085.1, SRR1803613.158.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGCGGC CCGAGGAAGG CAAGCAGTCG CCGCCGCCGC AGCCCTGGGG ACGGCTCCTG 
CGTCTGGGCG CGGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAGCGGGA GTGGACCATC 
GGGCGGAGAC GAGGTTGCGA CCTTTCCTTC CCCAGCAATA AACTGGTCTC TGGAGATCAC 
TGTAGAATTG TAGTGGATGA AAAATCAGGT CAGGTGACAC TGGAAGATAC CAGCACCAGT 
GGAACAGTGA TTAACAAGCT GAAGGTTGTT AAGAAGCAGA CATGCCCTTT ACAGACTGGG 
GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAACCGGAAC ACAACGTGGC ATACCTCTAT 
GAATCTTTAA GTGAAAAGCA AGGCATGACA CAAGAATCCT TTGATACCTC AGGTGCAGGT 
GCAGGGCGAG GGGCCGATCC CCGGGTCCCT CCGTCGTCGC CCGCCACTCA GGTGTGCTTT 
GAGGAACCAC AGCCATCAAC ATCGACGTCA GACCTCTTCC CCACAGCCTC GGCCTCTTCC 
ACGGAGCCTT CTCCTGCAGG GCGAGAGCGT TCCTCCAGTT GTGGGTCTGG GGGTGGTGGC 
ATCTCCCCTA AAGGAAGTGG TCCCTCTGTG GCAAGTGATG AAGTCTCCAG CTTTGCCTCA 
GCTCTCCCAG ACAGAAAGAC TGCGTCCTTT TCGTCGTTGG AACCCCAGGA TCAGGAGGAT 
TTGGAGCCCG TGAAGAAGAA AATGAGAGGA GATGGGGACC TTGACCTGAA CGGGCAGTTG 
TTGGTCGCAC AACCGCGTAG AAATGCCCAA ACCGTCCACG AGGACGTCAG AGCAGCGGCT 
GGGAAGCCAG ACAAGATGGA GGAGACGCTG ACATGCATCA TCTGCCAGGA CCTGCTGCAC 
GACTGCGTGA GTTTGCAGCC CTGCATGCAC ACGTTCTGCG CGGCTTGCTA CTCGGGCTGG 
ATGGAGCGCT CGTCCCTGTG TCCTACCTGC CGCTGTCCCG TGGAGCGGAT CTGTAAAAAC 
CACATCCTCA ACAACCTCGT GGAAGCATAC CTCATCCAGC ATCCAGACAA GAGTCGCAGT 
GAAGAAGATG TGCAAAGTAT GGATGCCAGG AATAAAATCA CTCAAGACAT GCTGCAGCCC 
AAAGTCAGGC GGTCTTTTTC TGATGAAGAA GGGAGTTCAG AGGACCTGCT GGAGCTGTCA 
GACGTTGACA GTGAGTCCTC AGACATTAGC CAGCCATACG TCGTGTGCCG GCAGTGTCCT 
GAGTACAGAA GGCAGGCGGC GCAGCCTCCC CACTGCCCAG CACCCGAGGG CGAGCCAGGA 
GCCCCACAGG CCCTGGGGGA TGCACCCTCC ACGTCCGTCA GCCTGACGAC AGCAGTCCAG 
GATTACGTGT GCCCTCTGCA AGGAAGCCAC GCCCTGTGCA CCTGCTGCTT CCAGCCCATG 
CCCGACCGGA GAGCGGAGCG CGAGCAGGAC CCGCGTGTCG CCCCTCAGCA GTGTGCGGTC 
TGCCTGCAGC CTTTCTGCCA CCTGTACTGG GGCTGCACCC GGACCGGCTG CTACGGCTGC 
CTGGCCCCGT TTTGTGAGCT CAACCTGGGT GACAAGTGTC TGGACGGCGT GCTGAACAAC 
AACAGCTACG AGTCAGACAT CCTGAAGAAT TACCTGGCAA CCAGAGGTTT GACATGGAAA 
AACATGTTGA CCGAGAGCCT CGTGGCTCTC CAGCGGGGAG TGTTTCTGCT GTCTGATTAC 
AGAGTCACGG GAGACACCGT TCTGTGTTAC TGCTGTGGCC TGCGCAGCTT CCGTGAGCTG 
ACCTATCAGT ATCGGCAGAA CATTCCTGCT TCCGAGTTGC CAGTGGCCGT AACATCCCGT 
CCTGACTGCT ACTGGGGCCG TAACTGCCGC ACTCAGGTGA AAGCTCACCA CGCCATGAAA 
TTCAATCATA TCTGTGAACA GACAAGGTTC AAAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001154818.1
CDS	85..2043
Translation	MERPEEGKQSPPPQPWGRLLRLGAEEGEPHVLLRKREWTIGRRRGCDLSFPSNKLVSGDHCRIVVDEKSGQVTLEDTSTSGTVINKLKVVKKQTCPLQTGDVIYLVYRKNEPEHNVAYLYESLSEKQGMTQESFDTSGAGAGRGADPRVPPSSPATQVCFEEPQPSTSTSDLFPTASASSTEPSPAGRERSSSCGSGGGGISPKGSGPSVASDEVSSFASALPDRKTASFSSLEPQDQEDLEPVKKKMRGDGDLDLNGQLLVAQPRRNAQTVHEDVRAAAGKPDKMEETLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSSLCPTCRCPVERICKNHILNNLVEAYLIQHPDKSRSEEDVQSMDARNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESSDISQPYVVCRQCPEYRRQAAQPPHCPAPEGEPGAPQALGDAPSTSVSLTTAVQDYVCPLQGSHALCTCCFQPMPDRRAEREQDPRVAPQQCAVCLQPFCHLYWGCTRTGCYGCLAPFCELNLGDKCLDGVLNNNSYESDILKNYLATRGLTWKNMLTESLVALQRGVFLLSDYRVTGDTVLCYCCGLRSFRELTYQYRQNIPASELPVAVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN

Target ORF information:

RefSeq Version	NM_001161346.1
Organism	Homo sapiens(human)
Definition	Homo sapiens checkpoint with forkhead and ring finger domains (CHFR), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001161346.1

ORF Insert Sequence:

ATGGAGCGGC CCGAGGAAGG CAAGCAGTCG CCGCCGCCGC AGCCCTGGGG ACGGCTCCTG 
CGTCTGGGCG CGGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAGCGGGA GTGGACCATC 
GGGCGGAGAC GAGGTTGCGA CCTTTCCTTC CCCAGCAATA AACTGGTCTC TGGAGATCAC 
TGTAGAATTG TAGTGGATGA AAAATCAGGT CAGGTGACAC TGGAAGATAC CAGCACCAGT 
GGAACAGTGA TTAACAAGCT GAAGGTTGTT AAGAAGCAGA CATGCCCTTT ACAGACTGGG 
GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAACCGGAAC ACAACGTGGC ATACCTCTAT 
GAATCTTTAA GTGAAAAGCA AGGCATGACA CAAGAATCCT TTGATACCTC AGGTGCAGGT 
GCAGGGCGAG GGGCCGATCC CCGGGTCCCT CCGTCGTCGC CCGCCACTCA GGTGTGCTTT 
GAGGAACCAC AGCCATCAAC ATCGACGTCA GACCTCTTCC CCACAGCCTC GGCCTCTTCC 
ACGGAGCCTT CTCCTGCAGG GCGAGAGCGT TCCTCCAGTT GTGGGTCTGG GGGTGGTGGC 
ATCTCCCCTA AAGGAAGTGG TCCCTCTGTG GCAAGTGATG AAGTCTCCAG CTTTGCCTCA 
GCTCTCCCAG ACAGAAAGAC TGCGTCCTTT TCGTCGTTGG AACCCCAGGA TCAGGAGGAT 
TTGGAGCCCG TGAAGAAGAA AATGAGAGGA GATGGGGACC TTGACCTGAA CGGGCAGTTG 
TTGGTCGCAC AACCGCGTAG AAATGCCCAA ACCGTCCACG AGGACGTCAG AGCAGCGGCT 
GGGAAGCCAG ACAAGATGGA GGAGACGCTG ACATGCATCA TCTGCCAGGA CCTGCTGCAC 
GACTGCGTGA GTTTGCAGCC CTGCATGCAC ACGTTCTGCG CGGCTTGCTA CTCGGGCTGG 
ATGGAGCGCT CGTCCCTGTG TCCTACCTGC CGCTGTCCCG TGGAGCGGAT CTGTAAAAAC 
CACATCCTCA ACAACCTCGT GGAAGCATAC CTCATCCAGC ATCCAGACAA GAGTCGCAGT 
GAAGAAGATG TGCAAAGTAT GGATGCCAGG AATAAAATCA CTCAAGACAT GCTGCAGCCC 
AAAGTCAGGC GGTCTTTTTC TGATGAAGAA GGGAGTTCAG AGGACCTGCT GGAGCTGTCA 
GACGTTGACA GTGAGTCCTC AGACATTAGC CAGCCATACG TCGTGTGCCG GCAGTGTCCT 
GAGTACAGAA GGCAGGCGGC GCAGCCTCCC CACTGCCCAG CACCCGAGGG CGAGCCAGGA 
GCCCCACAGG CCCTGGGGGA TGCACCCTCC ACGTCCGTCA GCCTGACGAC AGCAGTCCAG 
GATTACGTGT GCCCTCTGCA AGGAAGCCAC GCCCTGTGCA CCTGCTGCTT CCAGCCCATG 
CCCGACCGGA GAGCGGAGCG CGAGCAGGAC CCGCGTGTCG CCCCTCAGCA GTGTGCGGTC 
TGCCTGCAGC CTTTCTGCCA CCTGTACTGG GGCTGCACCC GGACCGGCTG CTACGGCTGC 
CTGGCCCCGT TTTGTGAGCT CAACCTGGGT GACAAGTGTC TGGACGGCGT GCTGAACAAC 
AACAGCTACG AGTCAGACAT CCTGAAGAAT TACCTGGCAA CCAGAGGTTT GACATGGAAA 
AACATGTTGA CCGAGAGCCT CGTGGCTCTC CAGCGGGGAG TGTTTCTGCT GTCTGATTAC 
AGAGTCACGG GAGACACCGT TCTGTGTTAC TGCTGTGGCC TGCGCAGCTT CCGTGAGCTG 
ACCTATCAGT ATCGGCAGAA CATTCCTGCT TCCGAGTTGC CAGTGGCCGT AACATCCCGT 
CCTGACTGCT ACTGGGGCCG TAACTGCCGC ACTCAGGTGA AAGCTCACCA CGCCATGAAA 
TTCAATCATA TCTGTGAACA GACAAGGTTC AAAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHFR ( NM_001161345.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001154817.1 Homo sapiens checkpoint with forkhead and ring finger domains (CHFR), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu15331
Clone ID Related Accession (Same CDS sequence)	NM_001161345.1
Accession Version	NM_001161345.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase CHFR isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP313700.1, AK304333.1, AK027687.1, AC127070.10 and BC012072.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK304333.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCGGC CCGAGGAAGG CAAGCAGTCG CCGCCGCCGC AGCCCTGGGG ACGGCTCCTG 
CGTCTGGGCG CGGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAGCGGGA GTGGACCATC 
GGGCGGAGAC GAGGTTGCGA CCTTTCCTTC CCCAGCAATA AACTGGTCTC TGGAGATCAC 
TGTAGAATTG TAGTGGATGA AAAATCAGGT CAGGTGACAC TGGAAGATAC CAGCACCAGT 
GGAACAGTGA TTAACAAGCT GAAGGTTGTT AAGAAGCAGA CATGCCCTTT ACAGACTGGG 
GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAACCGGAAC ACAACGTGGC ATACCTCTAT 
GAATCTTTAA GTGAAAAGCA AGGCATGACA CAAGAATCCT TTGAAGCTAA CAAGGAAAAT 
GTGTTCCATG GGACCAAAGA TACCTCAGGT GCAGGTGCAG GGCGAGGGGC CGATCCCCGG 
GTCCCTCCGT CGTCGCCCGC CACTCAGGTG TGCTTTGAGG AACCACAGCC ATCAACATCG 
ACGTCAGACC TCTTCCCCAC AGCCTCGGCC TCTTCCACGG AGCCTTCTCC TGCAGGGCGA 
GAGCGTTCCT CCAGTTGTGG GTCTGGGGGT GGTGGCATCT CCCCTAAAGG AAGTGGTCCC 
TCTGTGGCAA GTGATGAAGT CTCCAGCTTT GCCTCAGCTC TCCCAGACAG AAAGACTGCG 
TCCTTTTCGT CGTTGGAACC CCAGGATCAG GAGGATTTGG AGCCCGTGAA GAAGAAAATG 
AGAGGAGATG GGGACCTTGA CCTGAACGGG CAGTTGTTGG TCGCACAACC GCGTAGAAAT 
GCCCAAACCG TCCACGAGGA CGTCAGAGCA GCGGCTGGGA AGCCAGACAA GATGGAGGAG 
ACGCTGACAT GCATCATCTG CCAGGACCTG CTGCACGACT GCGTGAGTTT GCAGCCCTGC 
ATGCACACGT TCTGCGCGGC TTGCTACTCG GGCTGGATGG AGCGCTCGTC CCTGTGTCCT 
ACCTGCCGCT GTCCCGTGGA GCGGATCTGT AAAAACCACA TCCTCAACAA CCTCGTGGAA 
GCATACCTCA TCCAGCATCC AGACAAGAGT CGCAGTGAAG AAGATGTGCA AAGTATGGAT 
GCCAGGAATA AAATCACTCA AGACATGCTG CAGCCCAAAG TCAGGCGGTC TTTTTCTGAT 
GAAGAAGGGA GTTCAGAGGA CCTGCTGGAG CTGTCAGACG TTGACAGTGA GTCCTCAGAC 
ATTAGCCAGC CATACGTCGT GTGCCGGCAG TGTCCTGAGT ACAGAAGGCA GGCGGCGCAG 
CCTCCCCACT GCCCAGCACC CGAGGGCGAG CCAGGAGCCC CACAGGCCCT GGGGGATGCA 
CCCTCCACGT CCGTCAGCCT GACGACAGTC CAGGATTACG TGTGCCCTCT GCAAGGAAGC 
CACGCCCTGT GCACCTGCTG CTTCCAGCCC ATGCCCGACC GGAGAGCGGA GCGCGAGCAG 
GACCCGCGTG TCGCCCCTCA GCAGTGTGCG GTCTGCCTGC AGCCTTTCTG CCACCTGTAC 
TGGGGCTGCA CCCGGACCGG CTGCTACGGC TGCCTGGCCC CGTTTTGTGA GCTCAACCTG 
GGTGACAAGT GTCTGGACGG CGTGCTGAAC AACAACAGCT ACGAGTCAGA CATCCTGAAG 
AATTACCTGG CAACCAGAGG TTTGACATGG AAAAACATGT TGACCGAGAG CCTCGTGGCT 
CTCCAGCGGG GAGTGTTTCT GCTGTCTGAT TACAGAGTCA CGGGAGACAC CGTTCTGTGT 
TACTGCTGTG GCCTGCGCAG CTTCCGTGAG CTGACCTATC AGTATCGGCA GAACATTCCT 
GCTTCCGAGT TGCCAGTGGC CGTAACATCC CGTCCTGACT GCTACTGGGG CCGTAACTGC 
CGCACTCAGG TGAAAGCTCA CCACGCCATG AAATTCAATC ATATCTGTGA ACAGACAAGG 
TTCAAAAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001154817.1
CDS	85..2076
Translation	MERPEEGKQSPPPQPWGRLLRLGAEEGEPHVLLRKREWTIGRRRGCDLSFPSNKLVSGDHCRIVVDEKSGQVTLEDTSTSGTVINKLKVVKKQTCPLQTGDVIYLVYRKNEPEHNVAYLYESLSEKQGMTQESFEANKENVFHGTKDTSGAGAGRGADPRVPPSSPATQVCFEEPQPSTSTSDLFPTASASSTEPSPAGRERSSSCGSGGGGISPKGSGPSVASDEVSSFASALPDRKTASFSSLEPQDQEDLEPVKKKMRGDGDLDLNGQLLVAQPRRNAQTVHEDVRAAAGKPDKMEETLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSSLCPTCRCPVERICKNHILNNLVEAYLIQHPDKSRSEEDVQSMDARNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESSDISQPYVVCRQCPEYRRQAAQPPHCPAPEGEPGAPQALGDAPSTSVSLTTVQDYVCPLQGSHALCTCCFQPMPDRRAEREQDPRVAPQQCAVCLQPFCHLYWGCTRTGCYGCLAPFCELNLGDKCLDGVLNNNSYESDILKNYLATRGLTWKNMLTESLVALQRGVFLLSDYRVTGDTVLCYCCGLRSFRELTYQYRQNIPASELPVAVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN

Target ORF information:

RefSeq Version	NM_001161345.1
Organism	Homo sapiens(human)
Definition	Homo sapiens checkpoint with forkhead and ring finger domains (CHFR), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001161345.1

ORF Insert Sequence:

ATGGAGCGGC CCGAGGAAGG CAAGCAGTCG CCGCCGCCGC AGCCCTGGGG ACGGCTCCTG 
CGTCTGGGCG CGGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAGCGGGA GTGGACCATC 
GGGCGGAGAC GAGGTTGCGA CCTTTCCTTC CCCAGCAATA AACTGGTCTC TGGAGATCAC 
TGTAGAATTG TAGTGGATGA AAAATCAGGT CAGGTGACAC TGGAAGATAC CAGCACCAGT 
GGAACAGTGA TTAACAAGCT GAAGGTTGTT AAGAAGCAGA CATGCCCTTT ACAGACTGGG 
GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAACCGGAAC ACAACGTGGC ATACCTCTAT 
GAATCTTTAA GTGAAAAGCA AGGCATGACA CAAGAATCCT TTGAAGCTAA CAAGGAAAAT 
GTGTTCCATG GGACCAAAGA TACCTCAGGT GCAGGTGCAG GGCGAGGGGC CGATCCCCGG 
GTCCCTCCGT CGTCGCCCGC CACTCAGGTG TGCTTTGAGG AACCACAGCC ATCAACATCG 
ACGTCAGACC TCTTCCCCAC AGCCTCGGCC TCTTCCACGG AGCCTTCTCC TGCAGGGCGA 
GAGCGTTCCT CCAGTTGTGG GTCTGGGGGT GGTGGCATCT CCCCTAAAGG AAGTGGTCCC 
TCTGTGGCAA GTGATGAAGT CTCCAGCTTT GCCTCAGCTC TCCCAGACAG AAAGACTGCG 
TCCTTTTCGT CGTTGGAACC CCAGGATCAG GAGGATTTGG AGCCCGTGAA GAAGAAAATG 
AGAGGAGATG GGGACCTTGA CCTGAACGGG CAGTTGTTGG TCGCACAACC GCGTAGAAAT 
GCCCAAACCG TCCACGAGGA CGTCAGAGCA GCGGCTGGGA AGCCAGACAA GATGGAGGAG 
ACGCTGACAT GCATCATCTG CCAGGACCTG CTGCACGACT GCGTGAGTTT GCAGCCCTGC 
ATGCACACGT TCTGCGCGGC TTGCTACTCG GGCTGGATGG AGCGCTCGTC CCTGTGTCCT 
ACCTGCCGCT GTCCCGTGGA GCGGATCTGT AAAAACCACA TCCTCAACAA CCTCGTGGAA 
GCATACCTCA TCCAGCATCC AGACAAGAGT CGCAGTGAAG AAGATGTGCA AAGTATGGAT 
GCCAGGAATA AAATCACTCA AGACATGCTG CAGCCCAAAG TCAGGCGGTC TTTTTCTGAT 
GAAGAAGGGA GTTCAGAGGA CCTGCTGGAG CTGTCAGACG TTGACAGTGA GTCCTCAGAC 
ATTAGCCAGC CATACGTCGT GTGCCGGCAG TGTCCTGAGT ACAGAAGGCA GGCGGCGCAG 
CCTCCCCACT GCCCAGCACC CGAGGGCGAG CCAGGAGCCC CACAGGCCCT GGGGGATGCA 
CCCTCCACGT CCGTCAGCCT GACGACAGTC CAGGATTACG TGTGCCCTCT GCAAGGAAGC 
CACGCCCTGT GCACCTGCTG CTTCCAGCCC ATGCCCGACC GGAGAGCGGA GCGCGAGCAG 
GACCCGCGTG TCGCCCCTCA GCAGTGTGCG GTCTGCCTGC AGCCTTTCTG CCACCTGTAC 
TGGGGCTGCA CCCGGACCGG CTGCTACGGC TGCCTGGCCC CGTTTTGTGA GCTCAACCTG 
GGTGACAAGT GTCTGGACGG CGTGCTGAAC AACAACAGCT ACGAGTCAGA CATCCTGAAG 
AATTACCTGG CAACCAGAGG TTTGACATGG AAAAACATGT TGACCGAGAG CCTCGTGGCT 
CTCCAGCGGG GAGTGTTTCT GCTGTCTGAT TACAGAGTCA CGGGAGACAC CGTTCTGTGT 
TACTGCTGTG GCCTGCGCAG CTTCCGTGAG CTGACCTATC AGTATCGGCA GAACATTCCT 
GCTTCCGAGT TGCCAGTGGC CGTAACATCC CGTCCTGACT GCTACTGGGG CCGTAACTGC 
CGCACTCAGG TGAAAGCTCA CCACGCCATG AAATTCAATC ATATCTGTGA ACAGACAAGG 
TTCAAAAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHFR ( NM_001161347.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001154819.1 Homo sapiens checkpoint with forkhead and ring finger domains (CHFR), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu15351
Clone ID Related Accession (Same CDS sequence)	NM_001161347.1
Accession Version	NM_001161347.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-01
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase CHFR isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP313700.1, AK302785.1, AK027687.1, AC127070.10 and BC012072.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302785.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCGGC CCGAGGAAGG CAAGCAGTCG CCGCCGCCGC AGCCCTGGGG ACGGCTCCTG 
CGTCTGGGCG CGGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAGCGGGA GTGGACCATC 
GGGCGGAGAC GAGGTTGCGA CCTTTCCTTC CCCAGCAATA AACTGGTCTC TGGAGATCAC 
TGTAGAATTG TAGTGGATGA AAAATCAGGT CAGGTGACAC TGGAAGATAC CAGCACCAGT 
GGAACAGTGA TTAACAAGCT GAAGGTTGTT AAGAAGCAGA CATGCCCTTT ACAGACTGGG 
GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAACCGGAAC ACAGGTCTGG GGGTGGTGGC 
ATCTCCCCTA AAGGAAGTGG TCCCTCTGTG GCAAGTGATG AAGTCTCCAG CTTTGCCTCA 
GCTCTCCCAG ACAGAAAGAC TGCGTCCTTT TCGTCGTTGG AACCCCAGGA TCAGGAGGAT 
TTGGAGCCCG TGAAGAAGAA AATGAGAGGA GATGGGGACC TTGACCTGAA CGGGCAGTTG 
TTGGTCGCAC AACCGCGTAG AAATGCCCAA ACCGTCCACG AGGACGTCAG AGCAGCGGCT 
GGGAAGCCAG ACAAGATGGA GGAGACGCTG ACATGCATCA TCTGCCAGGA CCTGCTGCAC 
GACTGCGTGA GTTTGCAGCC CTGCATGCAC ACGTTCTGCG CGGCTTGCTA CTCGGGCTGG 
ATGGAGCGCT CGTCCCTGTG TCCTACCTGC CGCTGTCCCG TGGAGCGGAT CTGTAAAAAC 
CACATCCTCA ACAACCTCGT GGAAGCATAC CTCATCCAGC ATCCAGACAA GAGTCGCAGT 
GAAGAAGATG TGCAAAGTAT GGATGCCAGG AATAAAATCA CTCAAGACAT GCTGCAGCCC 
AAAGTCAGGC GGTCTTTTTC TGATGAAGAA GGGAGTTCAG AGGACCTGCT GGAGCTGTCA 
GACGTTGACA GTGAGTCCTC AGACATTAGC CAGCCATACG TCGTGTGCCG GCAGTGTCCT 
GAGTACAGAA GGCAGGCGGC GCAGCCTCCC CACTGCCCAG CACCCGAGGG CGAGCCAGGA 
GCCCCACAGG CCCTGGGGGA TGCACCCTCC ACGTCCGTCA GCCTGACGAC AGCAGTCCAG 
GATTACGTGT GCCCTCTGCA AGGAAGCCAC GCCCTGTGCA CCTGCTGCTT CCAGCCCATG 
CCCGACCGGA GAGCGGAGCG CGAGCAGGAC CCGCGTGTCG CCCCTCAGCA GTGTGCGGTC 
TGCCTGCAGC CTTTCTGCCA CCTGTACTGG GGCTGCACCC GGACCGGCTG CTACGGCTGC 
CTGGCCCCGT TTTGTGAGCT CAACCTGGGT GACAAGTGTC TGGACGGCGT GCTGAACAAC 
AACAGCTACG AGTCAGACAT CCTGAAGAAT TACCTGGCAA CCAGAGGTTT GACATGGAAA 
AACATGTTGA CCGAGAGCCT CGTGGCTCTC CAGCGGGGAG TGTTTCTGCT GTCTGATTAC 
AGAGTCACGG GAGACACCGT TCTGTGTTAC TGCTGTGGCC TGCGCAGCTT CCGTGAGCTG 
ACCTATCAGT ATCGGCAGAA CATTCCTGCT TCCGAGTTGC CAGTGGCCGT AACATCCCGT 
CCTGACTGCT ACTGGGGCCG TAACTGCCGC ACTCAGGTGA AAGCTCACCA CGCCATGAAA 
TTCAATCATA TCTGTGAACA GACAAGGTTC AAAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001154819.1
CDS	85..1803
Translation	MERPEEGKQSPPPQPWGRLLRLGAEEGEPHVLLRKREWTIGRRRGCDLSFPSNKLVSGDHCRIVVDEKSGQVTLEDTSTSGTVINKLKVVKKQTCPLQTGDVIYLVYRKNEPEHRSGGGGISPKGSGPSVASDEVSSFASALPDRKTASFSSLEPQDQEDLEPVKKKMRGDGDLDLNGQLLVAQPRRNAQTVHEDVRAAAGKPDKMEETLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSSLCPTCRCPVERICKNHILNNLVEAYLIQHPDKSRSEEDVQSMDARNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESSDISQPYVVCRQCPEYRRQAAQPPHCPAPEGEPGAPQALGDAPSTSVSLTTAVQDYVCPLQGSHALCTCCFQPMPDRRAEREQDPRVAPQQCAVCLQPFCHLYWGCTRTGCYGCLAPFCELNLGDKCLDGVLNNNSYESDILKNYLATRGLTWKNMLTESLVALQRGVFLLSDYRVTGDTVLCYCCGLRSFRELTYQYRQNIPASELPVAVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN

Target ORF information:

RefSeq Version	NM_001161347.1
Organism	Homo sapiens(human)
Definition	Homo sapiens checkpoint with forkhead and ring finger domains (CHFR), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001161347.1

ORF Insert Sequence:

ATGGAGCGGC CCGAGGAAGG CAAGCAGTCG CCGCCGCCGC AGCCCTGGGG ACGGCTCCTG 
CGTCTGGGCG CGGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAGCGGGA GTGGACCATC 
GGGCGGAGAC GAGGTTGCGA CCTTTCCTTC CCCAGCAATA AACTGGTCTC TGGAGATCAC 
TGTAGAATTG TAGTGGATGA AAAATCAGGT CAGGTGACAC TGGAAGATAC CAGCACCAGT 
GGAACAGTGA TTAACAAGCT GAAGGTTGTT AAGAAGCAGA CATGCCCTTT ACAGACTGGG 
GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAACCGGAAC ACAGGTCTGG GGGTGGTGGC 
ATCTCCCCTA AAGGAAGTGG TCCCTCTGTG GCAAGTGATG AAGTCTCCAG CTTTGCCTCA 
GCTCTCCCAG ACAGAAAGAC TGCGTCCTTT TCGTCGTTGG AACCCCAGGA TCAGGAGGAT 
TTGGAGCCCG TGAAGAAGAA AATGAGAGGA GATGGGGACC TTGACCTGAA CGGGCAGTTG 
TTGGTCGCAC AACCGCGTAG AAATGCCCAA ACCGTCCACG AGGACGTCAG AGCAGCGGCT 
GGGAAGCCAG ACAAGATGGA GGAGACGCTG ACATGCATCA TCTGCCAGGA CCTGCTGCAC 
GACTGCGTGA GTTTGCAGCC CTGCATGCAC ACGTTCTGCG CGGCTTGCTA CTCGGGCTGG 
ATGGAGCGCT CGTCCCTGTG TCCTACCTGC CGCTGTCCCG TGGAGCGGAT CTGTAAAAAC 
CACATCCTCA ACAACCTCGT GGAAGCATAC CTCATCCAGC ATCCAGACAA GAGTCGCAGT 
GAAGAAGATG TGCAAAGTAT GGATGCCAGG AATAAAATCA CTCAAGACAT GCTGCAGCCC 
AAAGTCAGGC GGTCTTTTTC TGATGAAGAA GGGAGTTCAG AGGACCTGCT GGAGCTGTCA 
GACGTTGACA GTGAGTCCTC AGACATTAGC CAGCCATACG TCGTGTGCCG GCAGTGTCCT 
GAGTACAGAA GGCAGGCGGC GCAGCCTCCC CACTGCCCAG CACCCGAGGG CGAGCCAGGA 
GCCCCACAGG CCCTGGGGGA TGCACCCTCC ACGTCCGTCA GCCTGACGAC AGCAGTCCAG 
GATTACGTGT GCCCTCTGCA AGGAAGCCAC GCCCTGTGCA CCTGCTGCTT CCAGCCCATG 
CCCGACCGGA GAGCGGAGCG CGAGCAGGAC CCGCGTGTCG CCCCTCAGCA GTGTGCGGTC 
TGCCTGCAGC CTTTCTGCCA CCTGTACTGG GGCTGCACCC GGACCGGCTG CTACGGCTGC 
CTGGCCCCGT TTTGTGAGCT CAACCTGGGT GACAAGTGTC TGGACGGCGT GCTGAACAAC 
AACAGCTACG AGTCAGACAT CCTGAAGAAT TACCTGGCAA CCAGAGGTTT GACATGGAAA 
AACATGTTGA CCGAGAGCCT CGTGGCTCTC CAGCGGGGAG TGTTTCTGCT GTCTGATTAC 
AGAGTCACGG GAGACACCGT TCTGTGTTAC TGCTGTGGCC TGCGCAGCTT CCGTGAGCTG 
ACCTATCAGT ATCGGCAGAA CATTCCTGCT TCCGAGTTGC CAGTGGCCGT AACATCCCGT 
CCTGACTGCT ACTGGGGCCG TAACTGCCGC ACTCAGGTGA AAGCTCACCA CGCCATGAAA 
TTCAATCATA TCTGTGAACA GACAAGGTTC AAAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHFR ( NM_001161346.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001154818.1 Homo sapiens checkpoint with forkhead and ring finger domains (CHFR), transcript variant 3, mRNA.

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CloneID	OHu109451
Clone ID Related Accession (Same CDS sequence)	NM_001161346.2
Accession Version	NM_001161346.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase CHFR isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC127070.10. On Aug 2, 2019 this sequence version replaced NM_001161346.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.125085.1, SRR1803615.188338.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGCGGC CCGAGGAAGG CAAGCAGTCG CCGCCGCCGC AGCCCTGGGG ACGGCTCCTG 
CGTCTGGGCG CGGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAGCGGGA GTGGACCATC 
GGGCGGAGAC GAGGTTGCGA CCTTTCCTTC CCCAGCAATA AACTGGTCTC TGGAGATCAC 
TGTAGAATTG TAGTGGATGA AAAATCAGGT CAGGTGACAC TGGAAGATAC CAGCACCAGT 
GGAACAGTGA TTAACAAGCT GAAGGTTGTT AAGAAGCAGA CATGCCCTTT ACAGACTGGG 
GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAACCGGAAC ACAACGTGGC ATACCTCTAT 
GAATCTTTAA GTGAAAAGCA AGGCATGACA CAAGAATCCT TTGATACCTC AGGTGCAGGT 
GCAGGGCGAG GGGCCGATCC CCGGGTCCCT CCGTCGTCGC CCGCCACTCA GGTGTGCTTT 
GAGGAACCAC AGCCATCAAC ATCGACGTCA GACCTCTTCC CCACAGCCTC GGCCTCTTCC 
ACGGAGCCTT CTCCTGCAGG GCGAGAGCGT TCCTCCAGTT GTGGGTCTGG GGGTGGTGGC 
ATCTCCCCTA AAGGAAGTGG TCCCTCTGTG GCAAGTGATG AAGTCTCCAG CTTTGCCTCA 
GCTCTCCCAG ACAGAAAGAC TGCGTCCTTT TCGTCGTTGG AACCCCAGGA TCAGGAGGAT 
TTGGAGCCCG TGAAGAAGAA AATGAGAGGA GATGGGGACC TTGACCTGAA CGGGCAGTTG 
TTGGTCGCAC AACCGCGTAG AAATGCCCAA ACCGTCCACG AGGACGTCAG AGCAGCGGCT 
GGGAAGCCAG ACAAGATGGA GGAGACGCTG ACATGCATCA TCTGCCAGGA CCTGCTGCAC 
GACTGCGTGA GTTTGCAGCC CTGCATGCAC ACGTTCTGCG CGGCTTGCTA CTCGGGCTGG 
ATGGAGCGCT CGTCCCTGTG TCCTACCTGC CGCTGTCCCG TGGAGCGGAT CTGTAAAAAC 
CACATCCTCA ACAACCTCGT GGAAGCATAC CTCATCCAGC ATCCAGACAA GAGTCGCAGT 
GAAGAAGATG TGCAAAGTAT GGATGCCAGA AATAAAATCA CTCAAGACAT GCTGCAGCCC 
AAAGTCAGGC GGTCTTTTTC TGATGAAGAA GGGAGTTCAG AGGACCTGCT GGAGCTGTCA 
GACGTTGACA GTGAGTCCTC AGACATTAGC CAGCCATACG TCGTGTGCCG GCAGTGTCCT 
GAGTACAGAA GGCAGGCGGC GCAGCCTCCC CACTGCCCAG CACCCGAGGG CGAGCCAGGA 
GCCCCACAGG CCCTGGGGGA TGCACCCTCC ACGTCCGTCA GCCTGACGAC AGCAGTCCAG 
GATTACGTGT GCCCTCTGCA AGGAAGCCAC GCCCTGTGCA CCTGCTGCTT CCAGCCCATG 
CCCGACCGGA GAGCGGAGCG CGAGCAGGAC CCGCGTGTCG CCCCTCAGCA GTGTGCGGTC 
TGCCTGCAGC CTTTCTGCCA CCTGTACTGG GGCTGCACCC GGACCGGCTG CTACGGCTGC 
CTGGCCCCGT TTTGTGAGCT CAACCTGGGT GACAAGTGTC TGGACGGCGT GCTGAACAAC 
AACAGCTACG AGTCAGACAT CCTGAAGAAT TACCTGGCAA CCAGAGGTTT GACATGGAAA 
AACATGTTGA CCGAGAGCCT CGTGGCTCTC CAGCGGGGAG TGTTTCTGCT GTCTGATTAC 
AGAGTCACGG GAGACACCGT TCTGTGTTAC TGCTGTGGCC TGCGCAGCTT CCGTGAGCTG 
ACCTATCAGT ATCGGCAGAA CATTCCTGCT TCCGAGTTGC CAGTGGCCGT AACATCCCGT 
CCTGACTGCT ACTGGGGCCG TAACTGCCGC ACTCAGGTGA AAGCTCACCA CGCCATGAAA 
TTCAATCATA TCTGTGAACA GACAAGGTTC AAAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001154818.1
CDS	72..2030
Translation	MERPEEGKQSPPPQPWGRLLRLGAEEGEPHVLLRKREWTIGRRRGCDLSFPSNKLVSGDHCRIVVDEKSGQVTLEDTSTSGTVINKLKVVKKQTCPLQTGDVIYLVYRKNEPEHNVAYLYESLSEKQGMTQESFDTSGAGAGRGADPRVPPSSPATQVCFEEPQPSTSTSDLFPTASASSTEPSPAGRERSSSCGSGGGGISPKGSGPSVASDEVSSFASALPDRKTASFSSLEPQDQEDLEPVKKKMRGDGDLDLNGQLLVAQPRRNAQTVHEDVRAAAGKPDKMEETLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSSLCPTCRCPVERICKNHILNNLVEAYLIQHPDKSRSEEDVQSMDARNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESSDISQPYVVCRQCPEYRRQAAQPPHCPAPEGEPGAPQALGDAPSTSVSLTTAVQDYVCPLQGSHALCTCCFQPMPDRRAEREQDPRVAPQQCAVCLQPFCHLYWGCTRTGCYGCLAPFCELNLGDKCLDGVLNNNSYESDILKNYLATRGLTWKNMLTESLVALQRGVFLLSDYRVTGDTVLCYCCGLRSFRELTYQYRQNIPASELPVAVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN

Target ORF information:

RefSeq Version	NM_001161346.2
Organism	Homo sapiens(human)
Definition	Homo sapiens checkpoint with forkhead and ring finger domains (CHFR), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001161346.2

ORF Insert Sequence:

ATGGAGCGGC CCGAGGAAGG CAAGCAGTCG CCGCCGCCGC AGCCCTGGGG ACGGCTCCTG 
CGTCTGGGCG CGGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAGCGGGA GTGGACCATC 
GGGCGGAGAC GAGGTTGCGA CCTTTCCTTC CCCAGCAATA AACTGGTCTC TGGAGATCAC 
TGTAGAATTG TAGTGGATGA AAAATCAGGT CAGGTGACAC TGGAAGATAC CAGCACCAGT 
GGAACAGTGA TTAACAAGCT GAAGGTTGTT AAGAAGCAGA CATGCCCTTT ACAGACTGGG 
GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAACCGGAAC ACAACGTGGC ATACCTCTAT 
GAATCTTTAA GTGAAAAGCA AGGCATGACA CAAGAATCCT TTGATACCTC AGGTGCAGGT 
GCAGGGCGAG GGGCCGATCC CCGGGTCCCT CCGTCGTCGC CCGCCACTCA GGTGTGCTTT 
GAGGAACCAC AGCCATCAAC ATCGACGTCA GACCTCTTCC CCACAGCCTC GGCCTCTTCC 
ACGGAGCCTT CTCCTGCAGG GCGAGAGCGT TCCTCCAGTT GTGGGTCTGG GGGTGGTGGC 
ATCTCCCCTA AAGGAAGTGG TCCCTCTGTG GCAAGTGATG AAGTCTCCAG CTTTGCCTCA 
GCTCTCCCAG ACAGAAAGAC TGCGTCCTTT TCGTCGTTGG AACCCCAGGA TCAGGAGGAT 
TTGGAGCCCG TGAAGAAGAA AATGAGAGGA GATGGGGACC TTGACCTGAA CGGGCAGTTG 
TTGGTCGCAC AACCGCGTAG AAATGCCCAA ACCGTCCACG AGGACGTCAG AGCAGCGGCT 
GGGAAGCCAG ACAAGATGGA GGAGACGCTG ACATGCATCA TCTGCCAGGA CCTGCTGCAC 
GACTGCGTGA GTTTGCAGCC CTGCATGCAC ACGTTCTGCG CGGCTTGCTA CTCGGGCTGG 
ATGGAGCGCT CGTCCCTGTG TCCTACCTGC CGCTGTCCCG TGGAGCGGAT CTGTAAAAAC 
CACATCCTCA ACAACCTCGT GGAAGCATAC CTCATCCAGC ATCCAGACAA GAGTCGCAGT 
GAAGAAGATG TGCAAAGTAT GGATGCCAGA AATAAAATCA CTCAAGACAT GCTGCAGCCC 
AAAGTCAGGC GGTCTTTTTC TGATGAAGAA GGGAGTTCAG AGGACCTGCT GGAGCTGTCA 
GACGTTGACA GTGAGTCCTC AGACATTAGC CAGCCATACG TCGTGTGCCG GCAGTGTCCT 
GAGTACAGAA GGCAGGCGGC GCAGCCTCCC CACTGCCCAG CACCCGAGGG CGAGCCAGGA 
GCCCCACAGG CCCTGGGGGA TGCACCCTCC ACGTCCGTCA GCCTGACGAC AGCAGTCCAG 
GATTACGTGT GCCCTCTGCA AGGAAGCCAC GCCCTGTGCA CCTGCTGCTT CCAGCCCATG 
CCCGACCGGA GAGCGGAGCG CGAGCAGGAC CCGCGTGTCG CCCCTCAGCA GTGTGCGGTC 
TGCCTGCAGC CTTTCTGCCA CCTGTACTGG GGCTGCACCC GGACCGGCTG CTACGGCTGC 
CTGGCCCCGT TTTGTGAGCT CAACCTGGGT GACAAGTGTC TGGACGGCGT GCTGAACAAC 
AACAGCTACG AGTCAGACAT CCTGAAGAAT TACCTGGCAA CCAGAGGTTT GACATGGAAA 
AACATGTTGA CCGAGAGCCT CGTGGCTCTC CAGCGGGGAG TGTTTCTGCT GTCTGATTAC 
AGAGTCACGG GAGACACCGT TCTGTGTTAC TGCTGTGGCC TGCGCAGCTT CCGTGAGCTG 
ACCTATCAGT ATCGGCAGAA CATTCCTGCT TCCGAGTTGC CAGTGGCCGT AACATCCCGT 
CCTGACTGCT ACTGGGGCCG TAACTGCCGC ACTCAGGTGA AAGCTCACCA CGCCATGAAA 
TTCAATCATA TCTGTGAACA GACAAGGTTC AAAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Methylation-independent CHFR expression is a potential biomarker affecting prognosis in acute myeloid leukemia.
Journal of cellular physiology(2017 Nov)
Zhou JD,Zhang TJ,Li XX,Ma JC,Guo H,Wen XM,Yao DM,Zhang W,Lin J,Qian J

Sustained expression of miR-26a promotes chromosomal instability and tumorigenesis through regulation of CHFR.
Nucleic acids research45(8)4401-4412(2017 May)
Castellano L,Dabrowska A,Pellegrino L,Ottaviani S,Cathcart P,Frampton AE,Krell J,Stebbing J

CHFR negatively regulates SIRT1 activity upon oxidative stress.
Scientific reports637578(2016 Nov)
Kim M,Kwon YE,Song JO,Bae SJ,Seol JH

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CHFR cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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