RAF1 cDNA ORF clone, Homo sapiens(human)

The following RAF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu28584 NM_001354690.2
Latest version!
Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu28584 NM_002880.3
Latest version!
Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu28584 XM_005265355.2
Latest version!
Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu28584 XM_011533974.3
Latest version!
Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu37400 NM_001354693.2
Latest version!
Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu37401 NM_001354691.2
Latest version!
Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu37401 NM_001354692.2
Latest version!
Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu37402 NM_001354695.2
Latest version!
Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu37400 XM_005265357.2
Latest version!
Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu37400 XM_017006966.1
Latest version!
Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu37401 XM_011533975.2
Latest version!
Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu37401 XM_005265358.4
Latest version!
Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu37402 XM_005265359.4
Latest version!
Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu103019 NM_001354689.3
Latest version!
Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu103097 NM_001354694.2
Latest version!
Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
View Old Accession Versions >>
OHu37401 NM_001354691.1 Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu28584 XM_011533974.2 Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu37401 NM_001354692.1 Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu103019 NM_001354689.1 Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu103097 NM_001354694.1 Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OHu103019 NM_001354689.2 Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu37402 NM_001354695.1 Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu28584 XM_005265355.1 Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu28584 XM_011533974.1 Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu28584 NM_001354690.1 Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu37400 NM_001354693.1 Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu28584
    Clone ID Related Accession (Same CDS sequence) XM_011533974.2 , NM_002880.3 , NM_001354690.1 , XM_011533974.3 , XM_005265355.1 , XM_005265355.2 , XM_011533974.1 , NM_001354690.2
    Accession Version NM_002880.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-07
    Organism Homo sapiens(human)
    Product RAF proto-oncogene serine/threonine-protein kinase isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC342671.1, BC018119.2 and BU683389.1. This sequence is a reference standard in the RefSeqGene project. On Jun 6, 2008 this sequence version replaced NM_002880.2. Transcript Variant: This variant (2), as well as variant 3, encodes isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.64938.1, SRR1803617.103457.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGCACA TACAGGGAGC TTGGAAGACG ATCAGCAATG GTTTTGGATT CAAAGATGCC 
    GTGTTTGATG GCTCCAGCTG CATCTCTCCT ACAATAGTTC AGCAGTTTGG CTATCAGCGC
    CGGGCATCAG ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT
    TTCTTGCCGA ACAAGCAAAG AACAGTGGTC AATGTGCGAA ATGGAATGAG CTTGCATGAC
    TGCCTTATGA AAGCACTCAA GGTGAGGGGC CTGCAACCAG AGTGCTGTGC AGTGTTCAGA
    CTTCTCCACG AACACAAAGG TAAAAAAGCA CGCTTAGATT GGAATACTGA TGCTGCGTCT
    TTGATTGGAG AAGAACTTCA AGTAGATTTC CTGGATCATG TTCCCCTCAC AACACACAAC
    TTTGCTCGGA AGACGTTCCT GAAGCTTGCC TTCTGTGACA TCTGTCAGAA ATTCCTGCTC
    AATGGATTTC GATGTCAGAC TTGTGGCTAC AAATTTCATG AGCACTGTAG CACCAAAGTA
    CCTACTATGT GTGTGGACTG GAGTAACATC AGACAACTCT TATTGTTTCC AAATTCCACT
    ATTGGTGATA GTGGAGTCCC AGCACTACCT TCTTTGACTA TGCGTCGTAT GCGAGAGTCT
    GTTTCCAGGA TGCCTGTTAG TTCTCAGCAC AGATATTCTA CACCTCACGC CTTCACCTTT
    AACACCTCCA GTCCCTCATC TGAAGGTTCC CTCTCCCAGA GGCAGAGGTC GACATCCACA
    CCTAATGTCC ACATGGTCAG CACCACCCTG CCTGTGGACA GCAGGATGAT TGAGGATGCA
    ATTCGAAGTC ACAGCGAATC AGCCTCACCT TCAGCCCTGT CCAGTAGCCC CAACAATCTG
    AGCCCAACAG GCTGGTCACA GCCGAAAACC CCCGTGCCAG CACAAAGAGA GCGGGCACCA
    GTATCTGGGA CCCAGGAGAA AAACAAAATT AGGCCTCGTG GACAGAGAGA TTCAAGCTAT
    TATTGGGAAA TAGAAGCCAG TGAAGTGATG CTGTCCACTC GGATTGGGTC AGGCTCTTTT
    GGAACTGTTT ATAAGGGTAA ATGGCACGGA GATGTTGCAG TAAAGATCCT AAAGGTTGTC
    GACCCAACCC CAGAGCAATT CCAGGCCTTC AGGAATGAGG TGGCTGTTCT GCGCAAAACA
    CGGCATGTGA ACATTCTGCT TTTCATGGGG TACATGACAA AGGACAACCT GGCAATTGTG
    ACCCAGTGGT GCGAGGGCAG CAGCCTCTAC AAACACCTGC ATGTCCAGGA GACCAAGTTT
    CAGATGTTCC AGCTAATTGA CATTGCCCGG CAGACGGCTC AGGGAATGGA CTATTTGCAT
    GCAAAGAACA TCATCCATAG AGACATGAAA TCCAACAATA TATTTCTCCA TGAAGGCTTA
    ACAGTGAAAA TTGGAGATTT TGGTTTGGCA ACAGTAAAGT CACGCTGGAG TGGTTCTCAG
    CAGGTTGAAC AACCTACTGG CTCTGTCCTC TGGATGGCCC CAGAGGTGAT CCGAATGCAG
    GATAACAACC CATTCAGTTT CCAGTCGGAT GTCTACTCCT ATGGCATCGT ATTGTATGAA
    CTGATGACGG GGGAGCTTCC TTATTCTCAC ATCAACAACC GAGATCAGAT CATCTTCATG
    GTGGGCCGAG GATATGCCTC CCCAGATCTT AGTAAGCTAT ATAAGAACTG CCCCAAAGCA
    ATGAAGAGGC TGGTAGCTGA CTGTGTGAAG AAAGTAAAGG AAGAGAGGCC TCTTTTTCCC
    CAGATCCTGT CTTCCATTGA GCTGCTCCAA CACTCTCTAC CGAAGATCAA CCGGAGCGCT
    TCCGAGCCAT CCTTGCATCG GGCAGCCCAC ACTGAGGATA TCAATGCTTG CACGCTGACC
    ACGTCCCCGA GGCTGCCTGT CTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_002871.1
    CDS416..2362
    Translation

    Target ORF information:

    RefSeq Version NM_002880.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_002880.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGCACA TACAGGGAGC TTGGAAGACG ATCAGCAATG GTTTTGGATT CAAAGATGCC 
    GTGTTTGATG GCTCCAGCTG CATCTCTCCT ACAATAGTTC AGCAGTTTGG CTATCAGCGC
    CGGGCATCAG ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT
    TTCTTGCCGA ACAAGCAAAG AACAGTGGTC AATGTGCGAA ATGGAATGAG CTTGCATGAC
    TGCCTTATGA AAGCACTCAA GGTGAGGGGC CTGCAACCAG AGTGCTGTGC AGTGTTCAGA
    CTTCTCCACG AACACAAAGG TAAAAAAGCA CGCTTAGATT GGAATACTGA TGCTGCGTCT
    TTGATTGGAG AAGAACTTCA AGTAGATTTC CTGGATCATG TTCCCCTCAC AACACACAAC
    TTTGCTCGGA AGACGTTCCT GAAGCTTGCC TTCTGTGACA TCTGTCAGAA ATTCCTGCTC
    AATGGATTTC GATGTCAGAC TTGTGGCTAC AAATTTCATG AGCACTGTAG CACCAAAGTA
    CCTACTATGT GTGTGGACTG GAGTAACATC AGACAACTCT TATTGTTTCC AAATTCCACT
    ATTGGTGATA GTGGAGTCCC AGCACTACCT TCTTTGACTA TGCGTCGTAT GCGAGAGTCT
    GTTTCCAGGA TGCCTGTTAG TTCTCAGCAC AGATATTCTA CACCTCACGC CTTCACCTTT
    AACACCTCCA GTCCCTCATC TGAAGGTTCC CTCTCCCAGA GGCAGAGGTC GACATCCACA
    CCTAATGTCC ACATGGTCAG CACCACCCTG CCTGTGGACA GCAGGATGAT TGAGGATGCA
    ATTCGAAGTC ACAGCGAATC AGCCTCACCT TCAGCCCTGT CCAGTAGCCC CAACAATCTG
    AGCCCAACAG GCTGGTCACA GCCGAAAACC CCCGTGCCAG CACAAAGAGA GCGGGCACCA
    GTATCTGGGA CCCAGGAGAA AAACAAAATT AGGCCTCGTG GACAGAGAGA TTCAAGCTAT
    TATTGGGAAA TAGAAGCCAG TGAAGTGATG CTGTCCACTC GGATTGGGTC AGGCTCTTTT
    GGAACTGTTT ATAAGGGTAA ATGGCACGGA GATGTTGCAG TAAAGATCCT AAAGGTTGTC
    GACCCAACCC CAGAGCAATT CCAGGCCTTC AGGAATGAGG TGGCTGTTCT GCGCAAAACA
    CGGCATGTGA ACATTCTGCT TTTCATGGGG TACATGACAA AGGACAACCT GGCAATTGTG
    ACCCAGTGGT GCGAGGGCAG CAGCCTCTAC AAACACCTGC ATGTCCAGGA GACCAAGTTT
    CAGATGTTCC AGCTAATTGA CATTGCCCGG CAGACGGCTC AGGGAATGGA CTATTTGCAT
    GCAAAGAACA TCATCCATAG AGACATGAAA TCCAACAATA TATTTCTCCA TGAAGGCTTA
    ACAGTGAAAA TTGGAGATTT TGGTTTGGCA ACAGTAAAGT CACGCTGGAG TGGTTCTCAG
    CAGGTTGAAC AACCTACTGG CTCTGTCCTC TGGATGGCCC CAGAGGTGAT CCGAATGCAG
    GATAACAACC CATTCAGTTT CCAGTCGGAT GTCTACTCCT ATGGCATCGT ATTGTATGAA
    CTGATGACGG GGGAGCTTCC TTATTCTCAC ATCAACAACC GAGATCAGAT CATCTTCATG
    GTGGGCCGAG GATATGCCTC CCCAGATCTT AGTAAGCTAT ATAAGAACTG CCCCAAAGCA
    ATGAAGAGGC TGGTAGCTGA CTGTGTGAAG AAAGTAAAGG AAGAGAGGCC TCTTTTTCCC
    CAGATCCTGT CTTCCATTGA GCTGCTCCAA CACTCTCTAC CGAAGATCAA CCGGAGCGCT
    TCCGAGCCAT CCTTGCATCG GGCAGCCCAC ACTGAGGATA TCAATGCTTG CACGCTGACC
    ACGTCCCCGA GGCTGCCTGT CTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28584
    Clone ID Related Accession (Same CDS sequence) XM_011533974.2 , NM_002880.3 , NM_001354690.1 , XM_011533974.3 , XM_005265355.1 , XM_005265355.2 , XM_011533974.1 , NM_001354690.2
    Accession Version NM_001354690.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product RAF proto-oncogene serine/threonine-protein kinase isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC026170.5 and AC018500.4. On Aug 18, 2017 this sequence version replaced XM_005265355.2. Transcript Variant: This variant (3), as well as variant 2, encodes isoform b. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.60548.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGCACA TACAGGGAGC TTGGAAGACG ATCAGCAATG GTTTTGGATT CAAAGATGCC 
    GTGTTTGATG GCTCCAGCTG CATCTCTCCT ACAATAGTTC AGCAGTTTGG CTATCAGCGC
    CGGGCATCAG ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT
    TTCTTGCCGA ACAAGCAAAG AACAGTGGTC AATGTGCGAA ATGGAATGAG CTTGCATGAC
    TGCCTTATGA AAGCACTCAA GGTGAGGGGC CTGCAACCAG AGTGCTGTGC AGTGTTCAGA
    CTTCTCCACG AACACAAAGG TAAAAAAGCA CGCTTAGATT GGAATACTGA TGCTGCGTCT
    TTGATTGGAG AAGAACTTCA AGTAGATTTC CTGGATCATG TTCCCCTCAC AACACACAAC
    TTTGCTCGGA AGACGTTCCT GAAGCTTGCC TTCTGTGACA TCTGTCAGAA ATTCCTGCTC
    AATGGATTTC GATGTCAGAC TTGTGGCTAC AAATTTCATG AGCACTGTAG CACCAAAGTA
    CCTACTATGT GTGTGGACTG GAGTAACATC AGACAACTCT TATTGTTTCC AAATTCCACT
    ATTGGTGATA GTGGAGTCCC AGCACTACCT TCTTTGACTA TGCGTCGTAT GCGAGAGTCT
    GTTTCCAGGA TGCCTGTTAG TTCTCAGCAC AGATATTCTA CACCTCACGC CTTCACCTTT
    AACACCTCCA GTCCCTCATC TGAAGGTTCC CTCTCCCAGA GGCAGAGGTC GACATCCACA
    CCTAATGTCC ACATGGTCAG CACCACCCTG CCTGTGGACA GCAGGATGAT TGAGGATGCA
    ATTCGAAGTC ACAGCGAATC AGCCTCACCT TCAGCCCTGT CCAGTAGCCC CAACAATCTG
    AGCCCAACAG GCTGGTCACA GCCGAAAACC CCCGTGCCAG CACAAAGAGA GCGGGCACCA
    GTATCTGGGA CCCAGGAGAA AAACAAAATT AGGCCTCGTG GACAGAGAGA TTCAAGCTAT
    TATTGGGAAA TAGAAGCCAG TGAAGTGATG CTGTCCACTC GGATTGGGTC AGGCTCTTTT
    GGAACTGTTT ATAAGGGTAA ATGGCACGGA GATGTTGCAG TAAAGATCCT AAAGGTTGTC
    GACCCAACCC CAGAGCAATT CCAGGCCTTC AGGAATGAGG TGGCTGTTCT GCGCAAAACA
    CGGCATGTGA ACATTCTGCT TTTCATGGGG TACATGACAA AGGACAACCT GGCAATTGTG
    ACCCAGTGGT GCGAGGGCAG CAGCCTCTAC AAACACCTGC ATGTCCAGGA GACCAAGTTT
    CAGATGTTCC AGCTAATTGA CATTGCCCGG CAGACGGCTC AGGGAATGGA CTATTTGCAT
    GCAAAGAACA TCATCCATAG AGACATGAAA TCCAACAATA TATTTCTCCA TGAAGGCTTA
    ACAGTGAAAA TTGGAGATTT TGGTTTGGCA ACAGTAAAGT CACGCTGGAG TGGTTCTCAG
    CAGGTTGAAC AACCTACTGG CTCTGTCCTC TGGATGGCCC CAGAGGTGAT CCGAATGCAG
    GATAACAACC CATTCAGTTT CCAGTCGGAT GTCTACTCCT ATGGCATCGT ATTGTATGAA
    CTGATGACGG GGGAGCTTCC TTATTCTCAC ATCAACAACC GAGATCAGAT CATCTTCATG
    GTGGGCCGAG GATATGCCTC CCCAGATCTT AGTAAGCTAT ATAAGAACTG CCCCAAAGCA
    ATGAAGAGGC TGGTAGCTGA CTGTGTGAAG AAAGTAAAGG AAGAGAGGCC TCTTTTTCCC
    CAGATCCTGT CTTCCATTGA GCTGCTCCAA CACTCTCTAC CGAAGATCAA CCGGAGCGCT
    TCCGAGCCAT CCTTGCATCG GGCAGCCCAC ACTGAGGATA TCAATGCTTG CACGCTGACC
    ACGTCCCCGA GGCTGCCTGT CTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001341619.1
    CDS167..2113
    Translation

    Target ORF information:

    RefSeq Version NM_001354690.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001354690.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGCACA TACAGGGAGC TTGGAAGACG ATCAGCAATG GTTTTGGATT CAAAGATGCC 
    GTGTTTGATG GCTCCAGCTG CATCTCTCCT ACAATAGTTC AGCAGTTTGG CTATCAGCGC
    CGGGCATCAG ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT
    TTCTTGCCGA ACAAGCAAAG AACAGTGGTC AATGTGCGAA ATGGAATGAG CTTGCATGAC
    TGCCTTATGA AAGCACTCAA GGTGAGGGGC CTGCAACCAG AGTGCTGTGC AGTGTTCAGA
    CTTCTCCACG AACACAAAGG TAAAAAAGCA CGCTTAGATT GGAATACTGA TGCTGCGTCT
    TTGATTGGAG AAGAACTTCA AGTAGATTTC CTGGATCATG TTCCCCTCAC AACACACAAC
    TTTGCTCGGA AGACGTTCCT GAAGCTTGCC TTCTGTGACA TCTGTCAGAA ATTCCTGCTC
    AATGGATTTC GATGTCAGAC TTGTGGCTAC AAATTTCATG AGCACTGTAG CACCAAAGTA
    CCTACTATGT GTGTGGACTG GAGTAACATC AGACAACTCT TATTGTTTCC AAATTCCACT
    ATTGGTGATA GTGGAGTCCC AGCACTACCT TCTTTGACTA TGCGTCGTAT GCGAGAGTCT
    GTTTCCAGGA TGCCTGTTAG TTCTCAGCAC AGATATTCTA CACCTCACGC CTTCACCTTT
    AACACCTCCA GTCCCTCATC TGAAGGTTCC CTCTCCCAGA GGCAGAGGTC GACATCCACA
    CCTAATGTCC ACATGGTCAG CACCACCCTG CCTGTGGACA GCAGGATGAT TGAGGATGCA
    ATTCGAAGTC ACAGCGAATC AGCCTCACCT TCAGCCCTGT CCAGTAGCCC CAACAATCTG
    AGCCCAACAG GCTGGTCACA GCCGAAAACC CCCGTGCCAG CACAAAGAGA GCGGGCACCA
    GTATCTGGGA CCCAGGAGAA AAACAAAATT AGGCCTCGTG GACAGAGAGA TTCAAGCTAT
    TATTGGGAAA TAGAAGCCAG TGAAGTGATG CTGTCCACTC GGATTGGGTC AGGCTCTTTT
    GGAACTGTTT ATAAGGGTAA ATGGCACGGA GATGTTGCAG TAAAGATCCT AAAGGTTGTC
    GACCCAACCC CAGAGCAATT CCAGGCCTTC AGGAATGAGG TGGCTGTTCT GCGCAAAACA
    CGGCATGTGA ACATTCTGCT TTTCATGGGG TACATGACAA AGGACAACCT GGCAATTGTG
    ACCCAGTGGT GCGAGGGCAG CAGCCTCTAC AAACACCTGC ATGTCCAGGA GACCAAGTTT
    CAGATGTTCC AGCTAATTGA CATTGCCCGG CAGACGGCTC AGGGAATGGA CTATTTGCAT
    GCAAAGAACA TCATCCATAG AGACATGAAA TCCAACAATA TATTTCTCCA TGAAGGCTTA
    ACAGTGAAAA TTGGAGATTT TGGTTTGGCA ACAGTAAAGT CACGCTGGAG TGGTTCTCAG
    CAGGTTGAAC AACCTACTGG CTCTGTCCTC TGGATGGCCC CAGAGGTGAT CCGAATGCAG
    GATAACAACC CATTCAGTTT CCAGTCGGAT GTCTACTCCT ATGGCATCGT ATTGTATGAA
    CTGATGACGG GGGAGCTTCC TTATTCTCAC ATCAACAACC GAGATCAGAT CATCTTCATG
    GTGGGCCGAG GATATGCCTC CCCAGATCTT AGTAAGCTAT ATAAGAACTG CCCCAAAGCA
    ATGAAGAGGC TGGTAGCTGA CTGTGTGAAG AAAGTAAAGG AAGAGAGGCC TCTTTTTCCC
    CAGATCCTGT CTTCCATTGA GCTGCTCCAA CACTCTCTAC CGAAGATCAA CCGGAGCGCT
    TCCGAGCCAT CCTTGCATCG GGCAGCCCAC ACTGAGGATA TCAATGCTTG CACGCTGACC
    ACGTCCCCGA GGCTGCCTGT CTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28584
    Clone ID Related Accession (Same CDS sequence) XM_011533974.2 , NM_002880.3 , NM_001354690.1 , XM_011533974.3 , XM_005265355.1 , XM_005265355.2 , XM_011533974.1 , NM_001354690.2
    Accession Version NM_001354690.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-07
    Organism Homo sapiens(human)
    Product RAF proto-oncogene serine/threonine-protein kinase isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC026170.5 and AC018500.4. On Jun 2, 2019 this sequence version replaced NM_001354690.1. Transcript Variant: This variant (3), as well as variant 2, encodes isoform b. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.60548.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGCACA TACAGGGAGC TTGGAAGACG ATCAGCAATG GTTTTGGATT CAAAGATGCC 
    GTGTTTGATG GCTCCAGCTG CATCTCTCCT ACAATAGTTC AGCAGTTTGG CTATCAGCGC
    CGGGCATCAG ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT
    TTCTTGCCGA ACAAGCAAAG AACAGTGGTC AATGTGCGAA ATGGAATGAG CTTGCATGAC
    TGCCTTATGA AAGCACTCAA GGTGAGGGGC CTGCAACCAG AGTGCTGTGC AGTGTTCAGA
    CTTCTCCACG AACACAAAGG TAAAAAAGCA CGCTTAGATT GGAATACTGA TGCTGCGTCT
    TTGATTGGAG AAGAACTTCA AGTAGATTTC CTGGATCATG TTCCCCTCAC AACACACAAC
    TTTGCTCGGA AGACGTTCCT GAAGCTTGCC TTCTGTGACA TCTGTCAGAA ATTCCTGCTC
    AATGGATTTC GATGTCAGAC TTGTGGCTAC AAATTTCATG AGCACTGTAG CACCAAAGTA
    CCTACTATGT GTGTGGACTG GAGTAACATC AGACAACTCT TATTGTTTCC AAATTCCACT
    ATTGGTGATA GTGGAGTCCC AGCACTACCT TCTTTGACTA TGCGTCGTAT GCGAGAGTCT
    GTTTCCAGGA TGCCTGTTAG TTCTCAGCAC AGATATTCTA CACCTCACGC CTTCACCTTT
    AACACCTCCA GTCCCTCATC TGAAGGTTCC CTCTCCCAGA GGCAGAGGTC GACATCCACA
    CCTAATGTCC ACATGGTCAG CACCACCCTG CCTGTGGACA GCAGGATGAT TGAGGATGCA
    ATTCGAAGTC ACAGCGAATC AGCCTCACCT TCAGCCCTGT CCAGTAGCCC CAACAATCTG
    AGCCCAACAG GCTGGTCACA GCCGAAAACC CCCGTGCCAG CACAAAGAGA GCGGGCACCA
    GTATCTGGGA CCCAGGAGAA AAACAAAATT AGGCCTCGTG GACAGAGAGA TTCAAGCTAT
    TATTGGGAAA TAGAAGCCAG TGAAGTGATG CTGTCCACTC GGATTGGGTC AGGCTCTTTT
    GGAACTGTTT ATAAGGGTAA ATGGCACGGA GATGTTGCAG TAAAGATCCT AAAGGTTGTC
    GACCCAACCC CAGAGCAATT CCAGGCCTTC AGGAATGAGG TGGCTGTTCT GCGCAAAACA
    CGGCATGTGA ACATTCTGCT TTTCATGGGG TACATGACAA AGGACAACCT GGCAATTGTG
    ACCCAGTGGT GCGAGGGCAG CAGCCTCTAC AAACACCTGC ATGTCCAGGA GACCAAGTTT
    CAGATGTTCC AGCTAATTGA CATTGCCCGG CAGACGGCTC AGGGAATGGA CTATTTGCAT
    GCAAAGAACA TCATCCATAG AGACATGAAA TCCAACAATA TATTTCTCCA TGAAGGCTTA
    ACAGTGAAAA TTGGAGATTT TGGTTTGGCA ACAGTAAAGT CACGCTGGAG TGGTTCTCAG
    CAGGTTGAAC AACCTACTGG CTCTGTCCTC TGGATGGCCC CAGAGGTGAT CCGAATGCAG
    GATAACAACC CATTCAGTTT CCAGTCGGAT GTCTACTCCT ATGGCATCGT ATTGTATGAA
    CTGATGACGG GGGAGCTTCC TTATTCTCAC ATCAACAACC GAGATCAGAT CATCTTCATG
    GTGGGCCGAG GATATGCCTC CCCAGATCTT AGTAAGCTAT ATAAGAACTG CCCCAAAGCA
    ATGAAGAGGC TGGTAGCTGA CTGTGTGAAG AAAGTAAAGG AAGAGAGGCC TCTTTTTCCC
    CAGATCCTGT CTTCCATTGA GCTGCTCCAA CACTCTCTAC CGAAGATCAA CCGGAGCGCT
    TCCGAGCCAT CCTTGCATCG GGCAGCCCAC ACTGAGGATA TCAATGCTTG CACGCTGACC
    ACGTCCCCGA GGCTGCCTGT CTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001341619.1
    CDS167..2113
    Translation

    Target ORF information:

    RefSeq Version NM_001354690.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001354690.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGCACA TACAGGGAGC TTGGAAGACG ATCAGCAATG GTTTTGGATT CAAAGATGCC 
    GTGTTTGATG GCTCCAGCTG CATCTCTCCT ACAATAGTTC AGCAGTTTGG CTATCAGCGC
    CGGGCATCAG ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT
    TTCTTGCCGA ACAAGCAAAG AACAGTGGTC AATGTGCGAA ATGGAATGAG CTTGCATGAC
    TGCCTTATGA AAGCACTCAA GGTGAGGGGC CTGCAACCAG AGTGCTGTGC AGTGTTCAGA
    CTTCTCCACG AACACAAAGG TAAAAAAGCA CGCTTAGATT GGAATACTGA TGCTGCGTCT
    TTGATTGGAG AAGAACTTCA AGTAGATTTC CTGGATCATG TTCCCCTCAC AACACACAAC
    TTTGCTCGGA AGACGTTCCT GAAGCTTGCC TTCTGTGACA TCTGTCAGAA ATTCCTGCTC
    AATGGATTTC GATGTCAGAC TTGTGGCTAC AAATTTCATG AGCACTGTAG CACCAAAGTA
    CCTACTATGT GTGTGGACTG GAGTAACATC AGACAACTCT TATTGTTTCC AAATTCCACT
    ATTGGTGATA GTGGAGTCCC AGCACTACCT TCTTTGACTA TGCGTCGTAT GCGAGAGTCT
    GTTTCCAGGA TGCCTGTTAG TTCTCAGCAC AGATATTCTA CACCTCACGC CTTCACCTTT
    AACACCTCCA GTCCCTCATC TGAAGGTTCC CTCTCCCAGA GGCAGAGGTC GACATCCACA
    CCTAATGTCC ACATGGTCAG CACCACCCTG CCTGTGGACA GCAGGATGAT TGAGGATGCA
    ATTCGAAGTC ACAGCGAATC AGCCTCACCT TCAGCCCTGT CCAGTAGCCC CAACAATCTG
    AGCCCAACAG GCTGGTCACA GCCGAAAACC CCCGTGCCAG CACAAAGAGA GCGGGCACCA
    GTATCTGGGA CCCAGGAGAA AAACAAAATT AGGCCTCGTG GACAGAGAGA TTCAAGCTAT
    TATTGGGAAA TAGAAGCCAG TGAAGTGATG CTGTCCACTC GGATTGGGTC AGGCTCTTTT
    GGAACTGTTT ATAAGGGTAA ATGGCACGGA GATGTTGCAG TAAAGATCCT AAAGGTTGTC
    GACCCAACCC CAGAGCAATT CCAGGCCTTC AGGAATGAGG TGGCTGTTCT GCGCAAAACA
    CGGCATGTGA ACATTCTGCT TTTCATGGGG TACATGACAA AGGACAACCT GGCAATTGTG
    ACCCAGTGGT GCGAGGGCAG CAGCCTCTAC AAACACCTGC ATGTCCAGGA GACCAAGTTT
    CAGATGTTCC AGCTAATTGA CATTGCCCGG CAGACGGCTC AGGGAATGGA CTATTTGCAT
    GCAAAGAACA TCATCCATAG AGACATGAAA TCCAACAATA TATTTCTCCA TGAAGGCTTA
    ACAGTGAAAA TTGGAGATTT TGGTTTGGCA ACAGTAAAGT CACGCTGGAG TGGTTCTCAG
    CAGGTTGAAC AACCTACTGG CTCTGTCCTC TGGATGGCCC CAGAGGTGAT CCGAATGCAG
    GATAACAACC CATTCAGTTT CCAGTCGGAT GTCTACTCCT ATGGCATCGT ATTGTATGAA
    CTGATGACGG GGGAGCTTCC TTATTCTCAC ATCAACAACC GAGATCAGAT CATCTTCATG
    GTGGGCCGAG GATATGCCTC CCCAGATCTT AGTAAGCTAT ATAAGAACTG CCCCAAAGCA
    ATGAAGAGGC TGGTAGCTGA CTGTGTGAAG AAAGTAAAGG AAGAGAGGCC TCTTTTTCCC
    CAGATCCTGT CTTCCATTGA GCTGCTCCAA CACTCTCTAC CGAAGATCAA CCGGAGCGCT
    TCCGAGCCAT CCTTGCATCG GGCAGCCCAC ACTGAGGATA TCAATGCTTG CACGCTGACC
    ACGTCCCCGA GGCTGCCTGT CTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28584
    Clone ID Related Accession (Same CDS sequence) XM_011533974.2 , NM_002880.3 , NM_001354690.1 , XM_011533974.3 , XM_005265355.1 , XM_005265355.2 , XM_011533974.1 , NM_001354690.2
    Accession Version XM_011533974.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product RAF proto-oncogene serine/threonine-protein kinase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011533974.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGCACA TACAGGGAGC TTGGAAGACG ATCAGCAATG GTTTTGGATT CAAAGATGCC 
    GTGTTTGATG GCTCCAGCTG CATCTCTCCT ACAATAGTTC AGCAGTTTGG CTATCAGCGC
    CGGGCATCAG ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT
    TTCTTGCCGA ACAAGCAAAG AACAGTGGTC AATGTGCGAA ATGGAATGAG CTTGCATGAC
    TGCCTTATGA AAGCACTCAA GGTGAGGGGC CTGCAACCAG AGTGCTGTGC AGTGTTCAGA
    CTTCTCCACG AACACAAAGG TAAAAAAGCA CGCTTAGATT GGAATACTGA TGCTGCGTCT
    TTGATTGGAG AAGAACTTCA AGTAGATTTC CTGGATCATG TTCCCCTCAC AACACACAAC
    TTTGCTCGGA AGACGTTCCT GAAGCTTGCC TTCTGTGACA TCTGTCAGAA ATTCCTGCTC
    AATGGATTTC GATGTCAGAC TTGTGGCTAC AAATTTCATG AGCACTGTAG CACCAAAGTA
    CCTACTATGT GTGTGGACTG GAGTAACATC AGACAACTCT TATTGTTTCC AAATTCCACT
    ATTGGTGATA GTGGAGTCCC AGCACTACCT TCTTTGACTA TGCGTCGTAT GCGAGAGTCT
    GTTTCCAGGA TGCCTGTTAG TTCTCAGCAC AGATATTCTA CACCTCACGC CTTCACCTTT
    AACACCTCCA GTCCCTCATC TGAAGGTTCC CTCTCCCAGA GGCAGAGGTC GACATCCACA
    CCTAATGTCC ACATGGTCAG CACCACCCTG CCTGTGGACA GCAGGATGAT TGAGGATGCA
    ATTCGAAGTC ACAGCGAATC AGCCTCACCT TCAGCCCTGT CCAGTAGCCC CAACAATCTG
    AGCCCAACAG GCTGGTCACA GCCGAAAACC CCCGTGCCAG CACAAAGAGA GCGGGCACCA
    GTATCTGGGA CCCAGGAGAA AAACAAAATT AGGCCTCGTG GACAGAGAGA TTCAAGCTAT
    TATTGGGAAA TAGAAGCCAG TGAAGTGATG CTGTCCACTC GGATTGGGTC AGGCTCTTTT
    GGAACTGTTT ATAAGGGTAA ATGGCACGGA GATGTTGCAG TAAAGATCCT AAAGGTTGTC
    GACCCAACCC CAGAGCAATT CCAGGCCTTC AGGAATGAGG TGGCTGTTCT GCGCAAAACA
    CGGCATGTGA ACATTCTGCT TTTCATGGGG TACATGACAA AGGACAACCT GGCAATTGTG
    ACCCAGTGGT GCGAGGGCAG CAGCCTCTAC AAACACCTGC ATGTCCAGGA GACCAAGTTT
    CAGATGTTCC AGCTAATTGA CATTGCCCGG CAGACGGCTC AGGGAATGGA CTATTTGCAT
    GCAAAGAACA TCATCCATAG AGACATGAAA TCCAACAATA TATTTCTCCA TGAAGGCTTA
    ACAGTGAAAA TTGGAGATTT TGGTTTGGCA ACAGTAAAGT CACGCTGGAG TGGTTCTCAG
    CAGGTTGAAC AACCTACTGG CTCTGTCCTC TGGATGGCCC CAGAGGTGAT CCGAATGCAG
    GATAACAACC CATTCAGTTT CCAGTCGGAT GTCTACTCCT ATGGCATCGT ATTGTATGAA
    CTGATGACGG GGGAGCTTCC TTATTCTCAC ATCAACAACC GAGATCAGAT CATCTTCATG
    GTGGGCCGAG GATATGCCTC CCCAGATCTT AGTAAGCTAT ATAAGAACTG CCCCAAAGCA
    ATGAAGAGGC TGGTAGCTGA CTGTGTGAAG AAAGTAAAGG AAGAGAGGCC TCTTTTTCCC
    CAGATCCTGT CTTCCATTGA GCTGCTCCAA CACTCTCTAC CGAAGATCAA CCGGAGCGCT
    TCCGAGCCAT CCTTGCATCG GGCAGCCCAC ACTGAGGATA TCAATGCTTG CACGCTGACC
    ACGTCCCCGA GGCTGCCTGT CTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011532276.1
    CDS459..2405
    Translation

    Target ORF information:

    RefSeq Version XM_011533974.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011533974.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGCACA TACAGGGAGC TTGGAAGACG ATCAGCAATG GTTTTGGATT CAAAGATGCC 
    GTGTTTGATG GCTCCAGCTG CATCTCTCCT ACAATAGTTC AGCAGTTTGG CTATCAGCGC
    CGGGCATCAG ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT
    TTCTTGCCGA ACAAGCAAAG AACAGTGGTC AATGTGCGAA ATGGAATGAG CTTGCATGAC
    TGCCTTATGA AAGCACTCAA GGTGAGGGGC CTGCAACCAG AGTGCTGTGC AGTGTTCAGA
    CTTCTCCACG AACACAAAGG TAAAAAAGCA CGCTTAGATT GGAATACTGA TGCTGCGTCT
    TTGATTGGAG AAGAACTTCA AGTAGATTTC CTGGATCATG TTCCCCTCAC AACACACAAC
    TTTGCTCGGA AGACGTTCCT GAAGCTTGCC TTCTGTGACA TCTGTCAGAA ATTCCTGCTC
    AATGGATTTC GATGTCAGAC TTGTGGCTAC AAATTTCATG AGCACTGTAG CACCAAAGTA
    CCTACTATGT GTGTGGACTG GAGTAACATC AGACAACTCT TATTGTTTCC AAATTCCACT
    ATTGGTGATA GTGGAGTCCC AGCACTACCT TCTTTGACTA TGCGTCGTAT GCGAGAGTCT
    GTTTCCAGGA TGCCTGTTAG TTCTCAGCAC AGATATTCTA CACCTCACGC CTTCACCTTT
    AACACCTCCA GTCCCTCATC TGAAGGTTCC CTCTCCCAGA GGCAGAGGTC GACATCCACA
    CCTAATGTCC ACATGGTCAG CACCACCCTG CCTGTGGACA GCAGGATGAT TGAGGATGCA
    ATTCGAAGTC ACAGCGAATC AGCCTCACCT TCAGCCCTGT CCAGTAGCCC CAACAATCTG
    AGCCCAACAG GCTGGTCACA GCCGAAAACC CCCGTGCCAG CACAAAGAGA GCGGGCACCA
    GTATCTGGGA CCCAGGAGAA AAACAAAATT AGGCCTCGTG GACAGAGAGA TTCAAGCTAT
    TATTGGGAAA TAGAAGCCAG TGAAGTGATG CTGTCCACTC GGATTGGGTC AGGCTCTTTT
    GGAACTGTTT ATAAGGGTAA ATGGCACGGA GATGTTGCAG TAAAGATCCT AAAGGTTGTC
    GACCCAACCC CAGAGCAATT CCAGGCCTTC AGGAATGAGG TGGCTGTTCT GCGCAAAACA
    CGGCATGTGA ACATTCTGCT TTTCATGGGG TACATGACAA AGGACAACCT GGCAATTGTG
    ACCCAGTGGT GCGAGGGCAG CAGCCTCTAC AAACACCTGC ATGTCCAGGA GACCAAGTTT
    CAGATGTTCC AGCTAATTGA CATTGCCCGG CAGACGGCTC AGGGAATGGA CTATTTGCAT
    GCAAAGAACA TCATCCATAG AGACATGAAA TCCAACAATA TATTTCTCCA TGAAGGCTTA
    ACAGTGAAAA TTGGAGATTT TGGTTTGGCA ACAGTAAAGT CACGCTGGAG TGGTTCTCAG
    CAGGTTGAAC AACCTACTGG CTCTGTCCTC TGGATGGCCC CAGAGGTGAT CCGAATGCAG
    GATAACAACC CATTCAGTTT CCAGTCGGAT GTCTACTCCT ATGGCATCGT ATTGTATGAA
    CTGATGACGG GGGAGCTTCC TTATTCTCAC ATCAACAACC GAGATCAGAT CATCTTCATG
    GTGGGCCGAG GATATGCCTC CCCAGATCTT AGTAAGCTAT ATAAGAACTG CCCCAAAGCA
    ATGAAGAGGC TGGTAGCTGA CTGTGTGAAG AAAGTAAAGG AAGAGAGGCC TCTTTTTCCC
    CAGATCCTGT CTTCCATTGA GCTGCTCCAA CACTCTCTAC CGAAGATCAA CCGGAGCGCT
    TCCGAGCCAT CCTTGCATCG GGCAGCCCAC ACTGAGGATA TCAATGCTTG CACGCTGACC
    ACGTCCCCGA GGCTGCCTGT CTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28584
    Clone ID Related Accession (Same CDS sequence) XM_011533974.2 , NM_002880.3 , NM_001354690.1 , XM_011533974.3 , XM_005265355.1 , XM_005265355.2 , XM_011533974.1 , NM_001354690.2
    Accession Version XM_011533974.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product RAF proto-oncogene serine/threonine-protein kinase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011533974.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGCACA TACAGGGAGC TTGGAAGACG ATCAGCAATG GTTTTGGATT CAAAGATGCC 
    GTGTTTGATG GCTCCAGCTG CATCTCTCCT ACAATAGTTC AGCAGTTTGG CTATCAGCGC
    CGGGCATCAG ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT
    TTCTTGCCGA ACAAGCAAAG AACAGTGGTC AATGTGCGAA ATGGAATGAG CTTGCATGAC
    TGCCTTATGA AAGCACTCAA GGTGAGGGGC CTGCAACCAG AGTGCTGTGC AGTGTTCAGA
    CTTCTCCACG AACACAAAGG TAAAAAAGCA CGCTTAGATT GGAATACTGA TGCTGCGTCT
    TTGATTGGAG AAGAACTTCA AGTAGATTTC CTGGATCATG TTCCCCTCAC AACACACAAC
    TTTGCTCGGA AGACGTTCCT GAAGCTTGCC TTCTGTGACA TCTGTCAGAA ATTCCTGCTC
    AATGGATTTC GATGTCAGAC TTGTGGCTAC AAATTTCATG AGCACTGTAG CACCAAAGTA
    CCTACTATGT GTGTGGACTG GAGTAACATC AGACAACTCT TATTGTTTCC AAATTCCACT
    ATTGGTGATA GTGGAGTCCC AGCACTACCT TCTTTGACTA TGCGTCGTAT GCGAGAGTCT
    GTTTCCAGGA TGCCTGTTAG TTCTCAGCAC AGATATTCTA CACCTCACGC CTTCACCTTT
    AACACCTCCA GTCCCTCATC TGAAGGTTCC CTCTCCCAGA GGCAGAGGTC GACATCCACA
    CCTAATGTCC ACATGGTCAG CACCACCCTG CCTGTGGACA GCAGGATGAT TGAGGATGCA
    ATTCGAAGTC ACAGCGAATC AGCCTCACCT TCAGCCCTGT CCAGTAGCCC CAACAATCTG
    AGCCCAACAG GCTGGTCACA GCCGAAAACC CCCGTGCCAG CACAAAGAGA GCGGGCACCA
    GTATCTGGGA CCCAGGAGAA AAACAAAATT AGGCCTCGTG GACAGAGAGA TTCAAGCTAT
    TATTGGGAAA TAGAAGCCAG TGAAGTGATG CTGTCCACTC GGATTGGGTC AGGCTCTTTT
    GGAACTGTTT ATAAGGGTAA ATGGCACGGA GATGTTGCAG TAAAGATCCT AAAGGTTGTC
    GACCCAACCC CAGAGCAATT CCAGGCCTTC AGGAATGAGG TGGCTGTTCT GCGCAAAACA
    CGGCATGTGA ACATTCTGCT TTTCATGGGG TACATGACAA AGGACAACCT GGCAATTGTG
    ACCCAGTGGT GCGAGGGCAG CAGCCTCTAC AAACACCTGC ATGTCCAGGA GACCAAGTTT
    CAGATGTTCC AGCTAATTGA CATTGCCCGG CAGACGGCTC AGGGAATGGA CTATTTGCAT
    GCAAAGAACA TCATCCATAG AGACATGAAA TCCAACAATA TATTTCTCCA TGAAGGCTTA
    ACAGTGAAAA TTGGAGATTT TGGTTTGGCA ACAGTAAAGT CACGCTGGAG TGGTTCTCAG
    CAGGTTGAAC AACCTACTGG CTCTGTCCTC TGGATGGCCC CAGAGGTGAT CCGAATGCAG
    GATAACAACC CATTCAGTTT CCAGTCGGAT GTCTACTCCT ATGGCATCGT ATTGTATGAA
    CTGATGACGG GGGAGCTTCC TTATTCTCAC ATCAACAACC GAGATCAGAT CATCTTCATG
    GTGGGCCGAG GATATGCCTC CCCAGATCTT AGTAAGCTAT ATAAGAACTG CCCCAAAGCA
    ATGAAGAGGC TGGTAGCTGA CTGTGTGAAG AAAGTAAAGG AAGAGAGGCC TCTTTTTCCC
    CAGATCCTGT CTTCCATTGA GCTGCTCCAA CACTCTCTAC CGAAGATCAA CCGGAGCGCT
    TCCGAGCCAT CCTTGCATCG GGCAGCCCAC ACTGAGGATA TCAATGCTTG CACGCTGACC
    ACGTCCCCGA GGCTGCCTGT CTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011532276.1
    CDS425..2371
    Translation

    Target ORF information:

    RefSeq Version XM_011533974.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011533974.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGCACA TACAGGGAGC TTGGAAGACG ATCAGCAATG GTTTTGGATT CAAAGATGCC 
    GTGTTTGATG GCTCCAGCTG CATCTCTCCT ACAATAGTTC AGCAGTTTGG CTATCAGCGC
    CGGGCATCAG ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT
    TTCTTGCCGA ACAAGCAAAG AACAGTGGTC AATGTGCGAA ATGGAATGAG CTTGCATGAC
    TGCCTTATGA AAGCACTCAA GGTGAGGGGC CTGCAACCAG AGTGCTGTGC AGTGTTCAGA
    CTTCTCCACG AACACAAAGG TAAAAAAGCA CGCTTAGATT GGAATACTGA TGCTGCGTCT
    TTGATTGGAG AAGAACTTCA AGTAGATTTC CTGGATCATG TTCCCCTCAC AACACACAAC
    TTTGCTCGGA AGACGTTCCT GAAGCTTGCC TTCTGTGACA TCTGTCAGAA ATTCCTGCTC
    AATGGATTTC GATGTCAGAC TTGTGGCTAC AAATTTCATG AGCACTGTAG CACCAAAGTA
    CCTACTATGT GTGTGGACTG GAGTAACATC AGACAACTCT TATTGTTTCC AAATTCCACT
    ATTGGTGATA GTGGAGTCCC AGCACTACCT TCTTTGACTA TGCGTCGTAT GCGAGAGTCT
    GTTTCCAGGA TGCCTGTTAG TTCTCAGCAC AGATATTCTA CACCTCACGC CTTCACCTTT
    AACACCTCCA GTCCCTCATC TGAAGGTTCC CTCTCCCAGA GGCAGAGGTC GACATCCACA
    CCTAATGTCC ACATGGTCAG CACCACCCTG CCTGTGGACA GCAGGATGAT TGAGGATGCA
    ATTCGAAGTC ACAGCGAATC AGCCTCACCT TCAGCCCTGT CCAGTAGCCC CAACAATCTG
    AGCCCAACAG GCTGGTCACA GCCGAAAACC CCCGTGCCAG CACAAAGAGA GCGGGCACCA
    GTATCTGGGA CCCAGGAGAA AAACAAAATT AGGCCTCGTG GACAGAGAGA TTCAAGCTAT
    TATTGGGAAA TAGAAGCCAG TGAAGTGATG CTGTCCACTC GGATTGGGTC AGGCTCTTTT
    GGAACTGTTT ATAAGGGTAA ATGGCACGGA GATGTTGCAG TAAAGATCCT AAAGGTTGTC
    GACCCAACCC CAGAGCAATT CCAGGCCTTC AGGAATGAGG TGGCTGTTCT GCGCAAAACA
    CGGCATGTGA ACATTCTGCT TTTCATGGGG TACATGACAA AGGACAACCT GGCAATTGTG
    ACCCAGTGGT GCGAGGGCAG CAGCCTCTAC AAACACCTGC ATGTCCAGGA GACCAAGTTT
    CAGATGTTCC AGCTAATTGA CATTGCCCGG CAGACGGCTC AGGGAATGGA CTATTTGCAT
    GCAAAGAACA TCATCCATAG AGACATGAAA TCCAACAATA TATTTCTCCA TGAAGGCTTA
    ACAGTGAAAA TTGGAGATTT TGGTTTGGCA ACAGTAAAGT CACGCTGGAG TGGTTCTCAG
    CAGGTTGAAC AACCTACTGG CTCTGTCCTC TGGATGGCCC CAGAGGTGAT CCGAATGCAG
    GATAACAACC CATTCAGTTT CCAGTCGGAT GTCTACTCCT ATGGCATCGT ATTGTATGAA
    CTGATGACGG GGGAGCTTCC TTATTCTCAC ATCAACAACC GAGATCAGAT CATCTTCATG
    GTGGGCCGAG GATATGCCTC CCCAGATCTT AGTAAGCTAT ATAAGAACTG CCCCAAAGCA
    ATGAAGAGGC TGGTAGCTGA CTGTGTGAAG AAAGTAAAGG AAGAGAGGCC TCTTTTTCCC
    CAGATCCTGT CTTCCATTGA GCTGCTCCAA CACTCTCTAC CGAAGATCAA CCGGAGCGCT
    TCCGAGCCAT CCTTGCATCG GGCAGCCCAC ACTGAGGATA TCAATGCTTG CACGCTGACC
    ACGTCCCCGA GGCTGCCTGT CTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28584
    Clone ID Related Accession (Same CDS sequence) XM_011533974.2 , NM_002880.3 , NM_001354690.1 , XM_011533974.3 , XM_005265355.1 , XM_005265355.2 , XM_011533974.1 , NM_001354690.2
    Accession Version XM_005265355.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product RAF proto-oncogene serine/threonine-protein kinase isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGCACA TACAGGGAGC TTGGAAGACG ATCAGCAATG GTTTTGGATT CAAAGATGCC 
    GTGTTTGATG GCTCCAGCTG CATCTCTCCT ACAATAGTTC AGCAGTTTGG CTATCAGCGC
    CGGGCATCAG ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT
    TTCTTGCCGA ACAAGCAAAG AACAGTGGTC AATGTGCGAA ATGGAATGAG CTTGCATGAC
    TGCCTTATGA AAGCACTCAA GGTGAGGGGC CTGCAACCAG AGTGCTGTGC AGTGTTCAGA
    CTTCTCCACG AACACAAAGG TAAAAAAGCA CGCTTAGATT GGAATACTGA TGCTGCGTCT
    TTGATTGGAG AAGAACTTCA AGTAGATTTC CTGGATCATG TTCCCCTCAC AACACACAAC
    TTTGCTCGGA AGACGTTCCT GAAGCTTGCC TTCTGTGACA TCTGTCAGAA ATTCCTGCTC
    AATGGATTTC GATGTCAGAC TTGTGGCTAC AAATTTCATG AGCACTGTAG CACCAAAGTA
    CCTACTATGT GTGTGGACTG GAGTAACATC AGACAACTCT TATTGTTTCC AAATTCCACT
    ATTGGTGATA GTGGAGTCCC AGCACTACCT TCTTTGACTA TGCGTCGTAT GCGAGAGTCT
    GTTTCCAGGA TGCCTGTTAG TTCTCAGCAC AGATATTCTA CACCTCACGC CTTCACCTTT
    AACACCTCCA GTCCCTCATC TGAAGGTTCC CTCTCCCAGA GGCAGAGGTC GACATCCACA
    CCTAATGTCC ACATGGTCAG CACCACCCTG CCTGTGGACA GCAGGATGAT TGAGGATGCA
    ATTCGAAGTC ACAGCGAATC AGCCTCACCT TCAGCCCTGT CCAGTAGCCC CAACAATCTG
    AGCCCAACAG GCTGGTCACA GCCGAAAACC CCCGTGCCAG CACAAAGAGA GCGGGCACCA
    GTATCTGGGA CCCAGGAGAA AAACAAAATT AGGCCTCGTG GACAGAGAGA TTCAAGCTAT
    TATTGGGAAA TAGAAGCCAG TGAAGTGATG CTGTCCACTC GGATTGGGTC AGGCTCTTTT
    GGAACTGTTT ATAAGGGTAA ATGGCACGGA GATGTTGCAG TAAAGATCCT AAAGGTTGTC
    GACCCAACCC CAGAGCAATT CCAGGCCTTC AGGAATGAGG TGGCTGTTCT GCGCAAAACA
    CGGCATGTGA ACATTCTGCT TTTCATGGGG TACATGACAA AGGACAACCT GGCAATTGTG
    ACCCAGTGGT GCGAGGGCAG CAGCCTCTAC AAACACCTGC ATGTCCAGGA GACCAAGTTT
    CAGATGTTCC AGCTAATTGA CATTGCCCGG CAGACGGCTC AGGGAATGGA CTATTTGCAT
    GCAAAGAACA TCATCCATAG AGACATGAAA TCCAACAATA TATTTCTCCA TGAAGGCTTA
    ACAGTGAAAA TTGGAGATTT TGGTTTGGCA ACAGTAAAGT CACGCTGGAG TGGTTCTCAG
    CAGGTTGAAC AACCTACTGG CTCTGTCCTC TGGATGGCCC CAGAGGTGAT CCGAATGCAG
    GATAACAACC CATTCAGTTT CCAGTCGGAT GTCTACTCCT ATGGCATCGT ATTGTATGAA
    CTGATGACGG GGGAGCTTCC TTATTCTCAC ATCAACAACC GAGATCAGAT CATCTTCATG
    GTGGGCCGAG GATATGCCTC CCCAGATCTT AGTAAGCTAT ATAAGAACTG CCCCAAAGCA
    ATGAAGAGGC TGGTAGCTGA CTGTGTGAAG AAAGTAAAGG AAGAGAGGCC TCTTTTTCCC
    CAGATCCTGT CTTCCATTGA GCTGCTCCAA CACTCTCTAC CGAAGATCAA CCGGAGCGCT
    TCCGAGCCAT CCTTGCATCG GGCAGCCCAC ACTGAGGATA TCAATGCTTG CACGCTGACC
    ACGTCCCCGA GGCTGCCTGT CTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005265412.1
    Translation

    Target ORF information:

    RefSeq Version XM_005265355.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005265355.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGCACA TACAGGGAGC TTGGAAGACG ATCAGCAATG GTTTTGGATT CAAAGATGCC 
    GTGTTTGATG GCTCCAGCTG CATCTCTCCT ACAATAGTTC AGCAGTTTGG CTATCAGCGC
    CGGGCATCAG ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT
    TTCTTGCCGA ACAAGCAAAG AACAGTGGTC AATGTGCGAA ATGGAATGAG CTTGCATGAC
    TGCCTTATGA AAGCACTCAA GGTGAGGGGC CTGCAACCAG AGTGCTGTGC AGTGTTCAGA
    CTTCTCCACG AACACAAAGG TAAAAAAGCA CGCTTAGATT GGAATACTGA TGCTGCGTCT
    TTGATTGGAG AAGAACTTCA AGTAGATTTC CTGGATCATG TTCCCCTCAC AACACACAAC
    TTTGCTCGGA AGACGTTCCT GAAGCTTGCC TTCTGTGACA TCTGTCAGAA ATTCCTGCTC
    AATGGATTTC GATGTCAGAC TTGTGGCTAC AAATTTCATG AGCACTGTAG CACCAAAGTA
    CCTACTATGT GTGTGGACTG GAGTAACATC AGACAACTCT TATTGTTTCC AAATTCCACT
    ATTGGTGATA GTGGAGTCCC AGCACTACCT TCTTTGACTA TGCGTCGTAT GCGAGAGTCT
    GTTTCCAGGA TGCCTGTTAG TTCTCAGCAC AGATATTCTA CACCTCACGC CTTCACCTTT
    AACACCTCCA GTCCCTCATC TGAAGGTTCC CTCTCCCAGA GGCAGAGGTC GACATCCACA
    CCTAATGTCC ACATGGTCAG CACCACCCTG CCTGTGGACA GCAGGATGAT TGAGGATGCA
    ATTCGAAGTC ACAGCGAATC AGCCTCACCT TCAGCCCTGT CCAGTAGCCC CAACAATCTG
    AGCCCAACAG GCTGGTCACA GCCGAAAACC CCCGTGCCAG CACAAAGAGA GCGGGCACCA
    GTATCTGGGA CCCAGGAGAA AAACAAAATT AGGCCTCGTG GACAGAGAGA TTCAAGCTAT
    TATTGGGAAA TAGAAGCCAG TGAAGTGATG CTGTCCACTC GGATTGGGTC AGGCTCTTTT
    GGAACTGTTT ATAAGGGTAA ATGGCACGGA GATGTTGCAG TAAAGATCCT AAAGGTTGTC
    GACCCAACCC CAGAGCAATT CCAGGCCTTC AGGAATGAGG TGGCTGTTCT GCGCAAAACA
    CGGCATGTGA ACATTCTGCT TTTCATGGGG TACATGACAA AGGACAACCT GGCAATTGTG
    ACCCAGTGGT GCGAGGGCAG CAGCCTCTAC AAACACCTGC ATGTCCAGGA GACCAAGTTT
    CAGATGTTCC AGCTAATTGA CATTGCCCGG CAGACGGCTC AGGGAATGGA CTATTTGCAT
    GCAAAGAACA TCATCCATAG AGACATGAAA TCCAACAATA TATTTCTCCA TGAAGGCTTA
    ACAGTGAAAA TTGGAGATTT TGGTTTGGCA ACAGTAAAGT CACGCTGGAG TGGTTCTCAG
    CAGGTTGAAC AACCTACTGG CTCTGTCCTC TGGATGGCCC CAGAGGTGAT CCGAATGCAG
    GATAACAACC CATTCAGTTT CCAGTCGGAT GTCTACTCCT ATGGCATCGT ATTGTATGAA
    CTGATGACGG GGGAGCTTCC TTATTCTCAC ATCAACAACC GAGATCAGAT CATCTTCATG
    GTGGGCCGAG GATATGCCTC CCCAGATCTT AGTAAGCTAT ATAAGAACTG CCCCAAAGCA
    ATGAAGAGGC TGGTAGCTGA CTGTGTGAAG AAAGTAAAGG AAGAGAGGCC TCTTTTTCCC
    CAGATCCTGT CTTCCATTGA GCTGCTCCAA CACTCTCTAC CGAAGATCAA CCGGAGCGCT
    TCCGAGCCAT CCTTGCATCG GGCAGCCCAC ACTGAGGATA TCAATGCTTG CACGCTGACC
    ACGTCCCCGA GGCTGCCTGT CTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28584
    Clone ID Related Accession (Same CDS sequence) XM_011533974.2 , NM_002880.3 , NM_001354690.1 , XM_011533974.3 , XM_005265355.1 , XM_005265355.2 , XM_011533974.1 , NM_001354690.2
    Accession Version XM_005265355.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(Human)
    Product RAF proto-oncogene serine/threonine-protein kinase isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005265355.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGCACA TACAGGGAGC TTGGAAGACG ATCAGCAATG GTTTTGGATT CAAAGATGCC 
    GTGTTTGATG GCTCCAGCTG CATCTCTCCT ACAATAGTTC AGCAGTTTGG CTATCAGCGC
    CGGGCATCAG ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT
    TTCTTGCCGA ACAAGCAAAG AACAGTGGTC AATGTGCGAA ATGGAATGAG CTTGCATGAC
    TGCCTTATGA AAGCACTCAA GGTGAGGGGC CTGCAACCAG AGTGCTGTGC AGTGTTCAGA
    CTTCTCCACG AACACAAAGG TAAAAAAGCA CGCTTAGATT GGAATACTGA TGCTGCGTCT
    TTGATTGGAG AAGAACTTCA AGTAGATTTC CTGGATCATG TTCCCCTCAC AACACACAAC
    TTTGCTCGGA AGACGTTCCT GAAGCTTGCC TTCTGTGACA TCTGTCAGAA ATTCCTGCTC
    AATGGATTTC GATGTCAGAC TTGTGGCTAC AAATTTCATG AGCACTGTAG CACCAAAGTA
    CCTACTATGT GTGTGGACTG GAGTAACATC AGACAACTCT TATTGTTTCC AAATTCCACT
    ATTGGTGATA GTGGAGTCCC AGCACTACCT TCTTTGACTA TGCGTCGTAT GCGAGAGTCT
    GTTTCCAGGA TGCCTGTTAG TTCTCAGCAC AGATATTCTA CACCTCACGC CTTCACCTTT
    AACACCTCCA GTCCCTCATC TGAAGGTTCC CTCTCCCAGA GGCAGAGGTC GACATCCACA
    CCTAATGTCC ACATGGTCAG CACCACCCTG CCTGTGGACA GCAGGATGAT TGAGGATGCA
    ATTCGAAGTC ACAGCGAATC AGCCTCACCT TCAGCCCTGT CCAGTAGCCC CAACAATCTG
    AGCCCAACAG GCTGGTCACA GCCGAAAACC CCCGTGCCAG CACAAAGAGA GCGGGCACCA
    GTATCTGGGA CCCAGGAGAA AAACAAAATT AGGCCTCGTG GACAGAGAGA TTCAAGCTAT
    TATTGGGAAA TAGAAGCCAG TGAAGTGATG CTGTCCACTC GGATTGGGTC AGGCTCTTTT
    GGAACTGTTT ATAAGGGTAA ATGGCACGGA GATGTTGCAG TAAAGATCCT AAAGGTTGTC
    GACCCAACCC CAGAGCAATT CCAGGCCTTC AGGAATGAGG TGGCTGTTCT GCGCAAAACA
    CGGCATGTGA ACATTCTGCT TTTCATGGGG TACATGACAA AGGACAACCT GGCAATTGTG
    ACCCAGTGGT GCGAGGGCAG CAGCCTCTAC AAACACCTGC ATGTCCAGGA GACCAAGTTT
    CAGATGTTCC AGCTAATTGA CATTGCCCGG CAGACGGCTC AGGGAATGGA CTATTTGCAT
    GCAAAGAACA TCATCCATAG AGACATGAAA TCCAACAATA TATTTCTCCA TGAAGGCTTA
    ACAGTGAAAA TTGGAGATTT TGGTTTGGCA ACAGTAAAGT CACGCTGGAG TGGTTCTCAG
    CAGGTTGAAC AACCTACTGG CTCTGTCCTC TGGATGGCCC CAGAGGTGAT CCGAATGCAG
    GATAACAACC CATTCAGTTT CCAGTCGGAT GTCTACTCCT ATGGCATCGT ATTGTATGAA
    CTGATGACGG GGGAGCTTCC TTATTCTCAC ATCAACAACC GAGATCAGAT CATCTTCATG
    GTGGGCCGAG GATATGCCTC CCCAGATCTT AGTAAGCTAT ATAAGAACTG CCCCAAAGCA
    ATGAAGAGGC TGGTAGCTGA CTGTGTGAAG AAAGTAAAGG AAGAGAGGCC TCTTTTTCCC
    CAGATCCTGT CTTCCATTGA GCTGCTCCAA CACTCTCTAC CGAAGATCAA CCGGAGCGCT
    TCCGAGCCAT CCTTGCATCG GGCAGCCCAC ACTGAGGATA TCAATGCTTG CACGCTGACC
    ACGTCCCCGA GGCTGCCTGT CTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005265412.1
    CDS144..2090
    Translation

    Target ORF information:

    RefSeq Version XM_005265355.2
    Organism Homo sapiens(Human)
    Definition Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005265355.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGCACA TACAGGGAGC TTGGAAGACG ATCAGCAATG GTTTTGGATT CAAAGATGCC 
    GTGTTTGATG GCTCCAGCTG CATCTCTCCT ACAATAGTTC AGCAGTTTGG CTATCAGCGC
    CGGGCATCAG ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT
    TTCTTGCCGA ACAAGCAAAG AACAGTGGTC AATGTGCGAA ATGGAATGAG CTTGCATGAC
    TGCCTTATGA AAGCACTCAA GGTGAGGGGC CTGCAACCAG AGTGCTGTGC AGTGTTCAGA
    CTTCTCCACG AACACAAAGG TAAAAAAGCA CGCTTAGATT GGAATACTGA TGCTGCGTCT
    TTGATTGGAG AAGAACTTCA AGTAGATTTC CTGGATCATG TTCCCCTCAC AACACACAAC
    TTTGCTCGGA AGACGTTCCT GAAGCTTGCC TTCTGTGACA TCTGTCAGAA ATTCCTGCTC
    AATGGATTTC GATGTCAGAC TTGTGGCTAC AAATTTCATG AGCACTGTAG CACCAAAGTA
    CCTACTATGT GTGTGGACTG GAGTAACATC AGACAACTCT TATTGTTTCC AAATTCCACT
    ATTGGTGATA GTGGAGTCCC AGCACTACCT TCTTTGACTA TGCGTCGTAT GCGAGAGTCT
    GTTTCCAGGA TGCCTGTTAG TTCTCAGCAC AGATATTCTA CACCTCACGC CTTCACCTTT
    AACACCTCCA GTCCCTCATC TGAAGGTTCC CTCTCCCAGA GGCAGAGGTC GACATCCACA
    CCTAATGTCC ACATGGTCAG CACCACCCTG CCTGTGGACA GCAGGATGAT TGAGGATGCA
    ATTCGAAGTC ACAGCGAATC AGCCTCACCT TCAGCCCTGT CCAGTAGCCC CAACAATCTG
    AGCCCAACAG GCTGGTCACA GCCGAAAACC CCCGTGCCAG CACAAAGAGA GCGGGCACCA
    GTATCTGGGA CCCAGGAGAA AAACAAAATT AGGCCTCGTG GACAGAGAGA TTCAAGCTAT
    TATTGGGAAA TAGAAGCCAG TGAAGTGATG CTGTCCACTC GGATTGGGTC AGGCTCTTTT
    GGAACTGTTT ATAAGGGTAA ATGGCACGGA GATGTTGCAG TAAAGATCCT AAAGGTTGTC
    GACCCAACCC CAGAGCAATT CCAGGCCTTC AGGAATGAGG TGGCTGTTCT GCGCAAAACA
    CGGCATGTGA ACATTCTGCT TTTCATGGGG TACATGACAA AGGACAACCT GGCAATTGTG
    ACCCAGTGGT GCGAGGGCAG CAGCCTCTAC AAACACCTGC ATGTCCAGGA GACCAAGTTT
    CAGATGTTCC AGCTAATTGA CATTGCCCGG CAGACGGCTC AGGGAATGGA CTATTTGCAT
    GCAAAGAACA TCATCCATAG AGACATGAAA TCCAACAATA TATTTCTCCA TGAAGGCTTA
    ACAGTGAAAA TTGGAGATTT TGGTTTGGCA ACAGTAAAGT CACGCTGGAG TGGTTCTCAG
    CAGGTTGAAC AACCTACTGG CTCTGTCCTC TGGATGGCCC CAGAGGTGAT CCGAATGCAG
    GATAACAACC CATTCAGTTT CCAGTCGGAT GTCTACTCCT ATGGCATCGT ATTGTATGAA
    CTGATGACGG GGGAGCTTCC TTATTCTCAC ATCAACAACC GAGATCAGAT CATCTTCATG
    GTGGGCCGAG GATATGCCTC CCCAGATCTT AGTAAGCTAT ATAAGAACTG CCCCAAAGCA
    ATGAAGAGGC TGGTAGCTGA CTGTGTGAAG AAAGTAAAGG AAGAGAGGCC TCTTTTTCCC
    CAGATCCTGT CTTCCATTGA GCTGCTCCAA CACTCTCTAC CGAAGATCAA CCGGAGCGCT
    TCCGAGCCAT CCTTGCATCG GGCAGCCCAC ACTGAGGATA TCAATGCTTG CACGCTGACC
    ACGTCCCCGA GGCTGCCTGT CTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28584
    Clone ID Related Accession (Same CDS sequence) XM_011533974.2 , NM_002880.3 , NM_001354690.1 , XM_011533974.3 , XM_005265355.1 , XM_005265355.2 , XM_011533974.1 , NM_001354690.2
    Accession Version XM_011533974.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product RAF proto-oncogene serine/threonine-protein kinase isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGCACA TACAGGGAGC TTGGAAGACG ATCAGCAATG GTTTTGGATT CAAAGATGCC 
    GTGTTTGATG GCTCCAGCTG CATCTCTCCT ACAATAGTTC AGCAGTTTGG CTATCAGCGC
    CGGGCATCAG ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT
    TTCTTGCCGA ACAAGCAAAG AACAGTGGTC AATGTGCGAA ATGGAATGAG CTTGCATGAC
    TGCCTTATGA AAGCACTCAA GGTGAGGGGC CTGCAACCAG AGTGCTGTGC AGTGTTCAGA
    CTTCTCCACG AACACAAAGG TAAAAAAGCA CGCTTAGATT GGAATACTGA TGCTGCGTCT
    TTGATTGGAG AAGAACTTCA AGTAGATTTC CTGGATCATG TTCCCCTCAC AACACACAAC
    TTTGCTCGGA AGACGTTCCT GAAGCTTGCC TTCTGTGACA TCTGTCAGAA ATTCCTGCTC
    AATGGATTTC GATGTCAGAC TTGTGGCTAC AAATTTCATG AGCACTGTAG CACCAAAGTA
    CCTACTATGT GTGTGGACTG GAGTAACATC AGACAACTCT TATTGTTTCC AAATTCCACT
    ATTGGTGATA GTGGAGTCCC AGCACTACCT TCTTTGACTA TGCGTCGTAT GCGAGAGTCT
    GTTTCCAGGA TGCCTGTTAG TTCTCAGCAC AGATATTCTA CACCTCACGC CTTCACCTTT
    AACACCTCCA GTCCCTCATC TGAAGGTTCC CTCTCCCAGA GGCAGAGGTC GACATCCACA
    CCTAATGTCC ACATGGTCAG CACCACCCTG CCTGTGGACA GCAGGATGAT TGAGGATGCA
    ATTCGAAGTC ACAGCGAATC AGCCTCACCT TCAGCCCTGT CCAGTAGCCC CAACAATCTG
    AGCCCAACAG GCTGGTCACA GCCGAAAACC CCCGTGCCAG CACAAAGAGA GCGGGCACCA
    GTATCTGGGA CCCAGGAGAA AAACAAAATT AGGCCTCGTG GACAGAGAGA TTCAAGCTAT
    TATTGGGAAA TAGAAGCCAG TGAAGTGATG CTGTCCACTC GGATTGGGTC AGGCTCTTTT
    GGAACTGTTT ATAAGGGTAA ATGGCACGGA GATGTTGCAG TAAAGATCCT AAAGGTTGTC
    GACCCAACCC CAGAGCAATT CCAGGCCTTC AGGAATGAGG TGGCTGTTCT GCGCAAAACA
    CGGCATGTGA ACATTCTGCT TTTCATGGGG TACATGACAA AGGACAACCT GGCAATTGTG
    ACCCAGTGGT GCGAGGGCAG CAGCCTCTAC AAACACCTGC ATGTCCAGGA GACCAAGTTT
    CAGATGTTCC AGCTAATTGA CATTGCCCGG CAGACGGCTC AGGGAATGGA CTATTTGCAT
    GCAAAGAACA TCATCCATAG AGACATGAAA TCCAACAATA TATTTCTCCA TGAAGGCTTA
    ACAGTGAAAA TTGGAGATTT TGGTTTGGCA ACAGTAAAGT CACGCTGGAG TGGTTCTCAG
    CAGGTTGAAC AACCTACTGG CTCTGTCCTC TGGATGGCCC CAGAGGTGAT CCGAATGCAG
    GATAACAACC CATTCAGTTT CCAGTCGGAT GTCTACTCCT ATGGCATCGT ATTGTATGAA
    CTGATGACGG GGGAGCTTCC TTATTCTCAC ATCAACAACC GAGATCAGAT CATCTTCATG
    GTGGGCCGAG GATATGCCTC CCCAGATCTT AGTAAGCTAT ATAAGAACTG CCCCAAAGCA
    ATGAAGAGGC TGGTAGCTGA CTGTGTGAAG AAAGTAAAGG AAGAGAGGCC TCTTTTTCCC
    CAGATCCTGT CTTCCATTGA GCTGCTCCAA CACTCTCTAC CGAAGATCAA CCGGAGCGCT
    TCCGAGCCAT CCTTGCATCG GGCAGCCCAC ACTGAGGATA TCAATGCTTG CACGCTGACC
    ACGTCCCCGA GGCTGCCTGT CTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011532276.1
    Translation

    Target ORF information:

    RefSeq Version XM_011533974.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011533974.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGCACA TACAGGGAGC TTGGAAGACG ATCAGCAATG GTTTTGGATT CAAAGATGCC 
    GTGTTTGATG GCTCCAGCTG CATCTCTCCT ACAATAGTTC AGCAGTTTGG CTATCAGCGC
    CGGGCATCAG ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT
    TTCTTGCCGA ACAAGCAAAG AACAGTGGTC AATGTGCGAA ATGGAATGAG CTTGCATGAC
    TGCCTTATGA AAGCACTCAA GGTGAGGGGC CTGCAACCAG AGTGCTGTGC AGTGTTCAGA
    CTTCTCCACG AACACAAAGG TAAAAAAGCA CGCTTAGATT GGAATACTGA TGCTGCGTCT
    TTGATTGGAG AAGAACTTCA AGTAGATTTC CTGGATCATG TTCCCCTCAC AACACACAAC
    TTTGCTCGGA AGACGTTCCT GAAGCTTGCC TTCTGTGACA TCTGTCAGAA ATTCCTGCTC
    AATGGATTTC GATGTCAGAC TTGTGGCTAC AAATTTCATG AGCACTGTAG CACCAAAGTA
    CCTACTATGT GTGTGGACTG GAGTAACATC AGACAACTCT TATTGTTTCC AAATTCCACT
    ATTGGTGATA GTGGAGTCCC AGCACTACCT TCTTTGACTA TGCGTCGTAT GCGAGAGTCT
    GTTTCCAGGA TGCCTGTTAG TTCTCAGCAC AGATATTCTA CACCTCACGC CTTCACCTTT
    AACACCTCCA GTCCCTCATC TGAAGGTTCC CTCTCCCAGA GGCAGAGGTC GACATCCACA
    CCTAATGTCC ACATGGTCAG CACCACCCTG CCTGTGGACA GCAGGATGAT TGAGGATGCA
    ATTCGAAGTC ACAGCGAATC AGCCTCACCT TCAGCCCTGT CCAGTAGCCC CAACAATCTG
    AGCCCAACAG GCTGGTCACA GCCGAAAACC CCCGTGCCAG CACAAAGAGA GCGGGCACCA
    GTATCTGGGA CCCAGGAGAA AAACAAAATT AGGCCTCGTG GACAGAGAGA TTCAAGCTAT
    TATTGGGAAA TAGAAGCCAG TGAAGTGATG CTGTCCACTC GGATTGGGTC AGGCTCTTTT
    GGAACTGTTT ATAAGGGTAA ATGGCACGGA GATGTTGCAG TAAAGATCCT AAAGGTTGTC
    GACCCAACCC CAGAGCAATT CCAGGCCTTC AGGAATGAGG TGGCTGTTCT GCGCAAAACA
    CGGCATGTGA ACATTCTGCT TTTCATGGGG TACATGACAA AGGACAACCT GGCAATTGTG
    ACCCAGTGGT GCGAGGGCAG CAGCCTCTAC AAACACCTGC ATGTCCAGGA GACCAAGTTT
    CAGATGTTCC AGCTAATTGA CATTGCCCGG CAGACGGCTC AGGGAATGGA CTATTTGCAT
    GCAAAGAACA TCATCCATAG AGACATGAAA TCCAACAATA TATTTCTCCA TGAAGGCTTA
    ACAGTGAAAA TTGGAGATTT TGGTTTGGCA ACAGTAAAGT CACGCTGGAG TGGTTCTCAG
    CAGGTTGAAC AACCTACTGG CTCTGTCCTC TGGATGGCCC CAGAGGTGAT CCGAATGCAG
    GATAACAACC CATTCAGTTT CCAGTCGGAT GTCTACTCCT ATGGCATCGT ATTGTATGAA
    CTGATGACGG GGGAGCTTCC TTATTCTCAC ATCAACAACC GAGATCAGAT CATCTTCATG
    GTGGGCCGAG GATATGCCTC CCCAGATCTT AGTAAGCTAT ATAAGAACTG CCCCAAAGCA
    ATGAAGAGGC TGGTAGCTGA CTGTGTGAAG AAAGTAAAGG AAGAGAGGCC TCTTTTTCCC
    CAGATCCTGT CTTCCATTGA GCTGCTCCAA CACTCTCTAC CGAAGATCAA CCGGAGCGCT
    TCCGAGCCAT CCTTGCATCG GGCAGCCCAC ACTGAGGATA TCAATGCTTG CACGCTGACC
    ACGTCCCCGA GGCTGCCTGT CTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu37400
    Clone ID Related Accession (Same CDS sequence) XM_005265357.2 , NM_001354693.1 , XM_017006966.1 , NM_001354693.2
    Accession Version NM_001354693.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1848bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product RAF proto-oncogene serine/threonine-protein kinase isoform d
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC026170.5 and AC018500.4. On Aug 29, 2017 this sequence version replaced XM_005265357.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.189501.1, SRR1660807.90303.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGAGCACA TACAGGGAGC TTGGAAGACG ATCAGCAATG GTTTTGGATT CAAAGATGCC 
    GTGTTTGATG GCTCCAGCTG CATCTCTCCT ACAATAGTTC AGCAGTTTGG CTATCAGCGC
    CGGGCATCAG ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT
    TTCTTGCCGA ACAAGCAAAG AACAGTGGTC AATGTGCGAA ATGGAATGAG CTTGCATGAC
    TGCCTTATGA AAGCACTCAA GGTGAGGGGC CTGCAACCAG AGTGCTGTGC AGTGTTCAGA
    CTTCTCCACG AACACAAAGG TAAAAAAGCA CGCTTAGATT GGAATACTGA TGCTGCGTCT
    TTGATTGGAG AAGAACTTCA AGTAGATTTC CTGGATCATG TTCCCCTCAC AACACACAAC
    TTTGCTCGGA AGACGTTCCT GAAGCTTGCC TTCTGTGACA TCTGTCAGAA ATTCCTGCTC
    AATGGATTTC GATGTCAGAC TTGTGGCTAC AAATTTCATG AGCACTGTAG CACCAAAGTA
    CCTACTATGT GTGTGGACTG GAGTAACATC AGACAACTCT TTTCTCAGCA CAGATATTCT
    ACACCTCACG CCTTCACCTT TAACACCTCC AGTCCCTCAT CTGAAGGTTC CCTCTCCCAG
    AGGCAGAGGT CGACATCCAC ACCTAATGTC CACATGGTCA GCACCACCCT GCCTGTGGAC
    AGCAGGATGA TTGAGGATGC AATTCGAAGT CACAGCGAAT CAGCCTCACC TTCAGCCCTG
    TCCAGTAGCC CCAACAATCT GAGCCCAACA GGCTGGTCAC AGCCGAAAAC CCCCGTGCCA
    GCACAAAGAG AGCGGGCACC AGTATCTGGG ACCCAGGAGA AAAACAAAAT TAGGCCTCGT
    GGACAGAGAG ATTCAAGCTA TTATTGGGAA ATAGAAGCCA GTGAAGTGAT GCTGTCCACT
    CGGATTGGGT CAGGCTCTTT TGGAACTGTT TATAAGGGTA AATGGCACGG AGATGTTGCA
    GTAAAGATCC TAAAGGTTGT CGACCCAACC CCAGAGCAAT TCCAGGCCTT CAGGAATGAG
    GTGGCTGTTC TGCGCAAAAC ACGGCATGTG AACATTCTGC TTTTCATGGG GTACATGACA
    AAGGACAACC TGGCAATTGT GACCCAGTGG TGCGAGGGCA GCAGCCTCTA CAAACACCTG
    CATGTCCAGG AGACCAAGTT TCAGATGTTC CAGCTAATTG ACATTGCCCG GCAGACGGCT
    CAGGGAATGG ACTATTTGCA TGCAAAGAAC ATCATCCATA GAGACATGAA ATCCAACAAT
    ATATTTCTCC ATGAAGGCTT AACAGTGAAA ATTGGAGATT TTGGTTTGGC AACAGTAAAG
    TCACGCTGGA GTGGTTCTCA GCAGGTTGAA CAACCTACTG GCTCTGTCCT CTGGATGGCC
    CCAGAGGTGA TCCGAATGCA GGATAACAAC CCATTCAGTT TCCAGTCGGA TGTCTACTCC
    TATGGCATCG TATTGTATGA ACTGATGACG GGGGAGCTTC CTTATTCTCA CATCAACAAC
    CGAGATCAGA TCATCTTCAT GGTGGGCCGA GGATATGCCT CCCCAGATCT TAGTAAGCTA
    TATAAGAACT GCCCCAAAGC AATGAAGAGG CTGGTAGCTG ACTGTGTGAA GAAAGTAAAG
    GAAGAGAGGC CTCTTTTTCC CCAGATCCTG TCTTCCATTG AGCTGCTCCA ACACTCTCTA
    CCGAAGATCA ACCGGAGCGC TTCCGAGCCA TCCTTGCATC GGGCAGCCCA CACTGAGGAT
    ATCAATGCTT GCACGCTGAC CACGTCCCCG AGGCTGCCTG TCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001341622.1
    CDS416..2263
    Translation

    Target ORF information:

    RefSeq Version NM_001354693.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001354693.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGAGCACA TACAGGGAGC TTGGAAGACG ATCAGCAATG GTTTTGGATT CAAAGATGCC 
    GTGTTTGATG GCTCCAGCTG CATCTCTCCT ACAATAGTTC AGCAGTTTGG CTATCAGCGC
    CGGGCATCAG ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT
    TTCTTGCCGA ACAAGCAAAG AACAGTGGTC AATGTGCGAA ATGGAATGAG CTTGCATGAC
    TGCCTTATGA AAGCACTCAA GGTGAGGGGC CTGCAACCAG AGTGCTGTGC AGTGTTCAGA
    CTTCTCCACG AACACAAAGG TAAAAAAGCA CGCTTAGATT GGAATACTGA TGCTGCGTCT
    TTGATTGGAG AAGAACTTCA AGTAGATTTC CTGGATCATG TTCCCCTCAC AACACACAAC
    TTTGCTCGGA AGACGTTCCT GAAGCTTGCC TTCTGTGACA TCTGTCAGAA ATTCCTGCTC
    AATGGATTTC GATGTCAGAC TTGTGGCTAC AAATTTCATG AGCACTGTAG CACCAAAGTA
    CCTACTATGT GTGTGGACTG GAGTAACATC AGACAACTCT TTTCTCAGCA CAGATATTCT
    ACACCTCACG CCTTCACCTT TAACACCTCC AGTCCCTCAT CTGAAGGTTC CCTCTCCCAG
    AGGCAGAGGT CGACATCCAC ACCTAATGTC CACATGGTCA GCACCACCCT GCCTGTGGAC
    AGCAGGATGA TTGAGGATGC AATTCGAAGT CACAGCGAAT CAGCCTCACC TTCAGCCCTG
    TCCAGTAGCC CCAACAATCT GAGCCCAACA GGCTGGTCAC AGCCGAAAAC CCCCGTGCCA
    GCACAAAGAG AGCGGGCACC AGTATCTGGG ACCCAGGAGA AAAACAAAAT TAGGCCTCGT
    GGACAGAGAG ATTCAAGCTA TTATTGGGAA ATAGAAGCCA GTGAAGTGAT GCTGTCCACT
    CGGATTGGGT CAGGCTCTTT TGGAACTGTT TATAAGGGTA AATGGCACGG AGATGTTGCA
    GTAAAGATCC TAAAGGTTGT CGACCCAACC CCAGAGCAAT TCCAGGCCTT CAGGAATGAG
    GTGGCTGTTC TGCGCAAAAC ACGGCATGTG AACATTCTGC TTTTCATGGG GTACATGACA
    AAGGACAACC TGGCAATTGT GACCCAGTGG TGCGAGGGCA GCAGCCTCTA CAAACACCTG
    CATGTCCAGG AGACCAAGTT TCAGATGTTC CAGCTAATTG ACATTGCCCG GCAGACGGCT
    CAGGGAATGG ACTATTTGCA TGCAAAGAAC ATCATCCATA GAGACATGAA ATCCAACAAT
    ATATTTCTCC ATGAAGGCTT AACAGTGAAA ATTGGAGATT TTGGTTTGGC AACAGTAAAG
    TCACGCTGGA GTGGTTCTCA GCAGGTTGAA CAACCTACTG GCTCTGTCCT CTGGATGGCC
    CCAGAGGTGA TCCGAATGCA GGATAACAAC CCATTCAGTT TCCAGTCGGA TGTCTACTCC
    TATGGCATCG TATTGTATGA ACTGATGACG GGGGAGCTTC CTTATTCTCA CATCAACAAC
    CGAGATCAGA TCATCTTCAT GGTGGGCCGA GGATATGCCT CCCCAGATCT TAGTAAGCTA
    TATAAGAACT GCCCCAAAGC AATGAAGAGG CTGGTAGCTG ACTGTGTGAA GAAAGTAAAG
    GAAGAGAGGC CTCTTTTTCC CCAGATCCTG TCTTCCATTG AGCTGCTCCA ACACTCTCTA
    CCGAAGATCA ACCGGAGCGC TTCCGAGCCA TCCTTGCATC GGGCAGCCCA CACTGAGGAT
    ATCAATGCTT GCACGCTGAC CACGTCCCCG AGGCTGCCTG TCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu37400
    Clone ID Related Accession (Same CDS sequence) XM_005265357.2 , NM_001354693.1 , XM_017006966.1 , NM_001354693.2
    Accession Version NM_001354693.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1848bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-07
    Organism Homo sapiens(human)
    Product RAF proto-oncogene serine/threonine-protein kinase isoform d
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC026170.5 and AC018500.4. On Jun 2, 2019 this sequence version replaced NM_001354693.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.137096.1, SRR1660807.90303.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGAGCACA TACAGGGAGC TTGGAAGACG ATCAGCAATG GTTTTGGATT CAAAGATGCC 
    GTGTTTGATG GCTCCAGCTG CATCTCTCCT ACAATAGTTC AGCAGTTTGG CTATCAGCGC
    CGGGCATCAG ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT
    TTCTTGCCGA ACAAGCAAAG AACAGTGGTC AATGTGCGAA ATGGAATGAG CTTGCATGAC
    TGCCTTATGA AAGCACTCAA GGTGAGGGGC CTGCAACCAG AGTGCTGTGC AGTGTTCAGA
    CTTCTCCACG AACACAAAGG TAAAAAAGCA CGCTTAGATT GGAATACTGA TGCTGCGTCT
    TTGATTGGAG AAGAACTTCA AGTAGATTTC CTGGATCATG TTCCCCTCAC AACACACAAC
    TTTGCTCGGA AGACGTTCCT GAAGCTTGCC TTCTGTGACA TCTGTCAGAA ATTCCTGCTC
    AATGGATTTC GATGTCAGAC TTGTGGCTAC AAATTTCATG AGCACTGTAG CACCAAAGTA
    CCTACTATGT GTGTGGACTG GAGTAACATC AGACAACTCT TTTCTCAGCA CAGATATTCT
    ACACCTCACG CCTTCACCTT TAACACCTCC AGTCCCTCAT CTGAAGGTTC CCTCTCCCAG
    AGGCAGAGGT CGACATCCAC ACCTAATGTC CACATGGTCA GCACCACCCT GCCTGTGGAC
    AGCAGGATGA TTGAGGATGC AATTCGAAGT CACAGCGAAT CAGCCTCACC TTCAGCCCTG
    TCCAGTAGCC CCAACAATCT GAGCCCAACA GGCTGGTCAC AGCCGAAAAC CCCCGTGCCA
    GCACAAAGAG AGCGGGCACC AGTATCTGGG ACCCAGGAGA AAAACAAAAT TAGGCCTCGT
    GGACAGAGAG ATTCAAGCTA TTATTGGGAA ATAGAAGCCA GTGAAGTGAT GCTGTCCACT
    CGGATTGGGT CAGGCTCTTT TGGAACTGTT TATAAGGGTA AATGGCACGG AGATGTTGCA
    GTAAAGATCC TAAAGGTTGT CGACCCAACC CCAGAGCAAT TCCAGGCCTT CAGGAATGAG
    GTGGCTGTTC TGCGCAAAAC ACGGCATGTG AACATTCTGC TTTTCATGGG GTACATGACA
    AAGGACAACC TGGCAATTGT GACCCAGTGG TGCGAGGGCA GCAGCCTCTA CAAACACCTG
    CATGTCCAGG AGACCAAGTT TCAGATGTTC CAGCTAATTG ACATTGCCCG GCAGACGGCT
    CAGGGAATGG ACTATTTGCA TGCAAAGAAC ATCATCCATA GAGACATGAA ATCCAACAAT
    ATATTTCTCC ATGAAGGCTT AACAGTGAAA ATTGGAGATT TTGGTTTGGC AACAGTAAAG
    TCACGCTGGA GTGGTTCTCA GCAGGTTGAA CAACCTACTG GCTCTGTCCT CTGGATGGCC
    CCAGAGGTGA TCCGAATGCA GGATAACAAC CCATTCAGTT TCCAGTCGGA TGTCTACTCC
    TATGGCATCG TATTGTATGA ACTGATGACG GGGGAGCTTC CTTATTCTCA CATCAACAAC
    CGAGATCAGA TCATCTTCAT GGTGGGCCGA GGATATGCCT CCCCAGATCT TAGTAAGCTA
    TATAAGAACT GCCCCAAAGC AATGAAGAGG CTGGTAGCTG ACTGTGTGAA GAAAGTAAAG
    GAAGAGAGGC CTCTTTTTCC CCAGATCCTG TCTTCCATTG AGCTGCTCCA ACACTCTCTA
    CCGAAGATCA ACCGGAGCGC TTCCGAGCCA TCCTTGCATC GGGCAGCCCA CACTGAGGAT
    ATCAATGCTT GCACGCTGAC CACGTCCCCG AGGCTGCCTG TCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001341622.1
    CDS332..2179
    Translation

    Target ORF information:

    RefSeq Version NM_001354693.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001354693.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGAGCACA TACAGGGAGC TTGGAAGACG ATCAGCAATG GTTTTGGATT CAAAGATGCC 
    GTGTTTGATG GCTCCAGCTG CATCTCTCCT ACAATAGTTC AGCAGTTTGG CTATCAGCGC
    CGGGCATCAG ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT
    TTCTTGCCGA ACAAGCAAAG AACAGTGGTC AATGTGCGAA ATGGAATGAG CTTGCATGAC
    TGCCTTATGA AAGCACTCAA GGTGAGGGGC CTGCAACCAG AGTGCTGTGC AGTGTTCAGA
    CTTCTCCACG AACACAAAGG TAAAAAAGCA CGCTTAGATT GGAATACTGA TGCTGCGTCT
    TTGATTGGAG AAGAACTTCA AGTAGATTTC CTGGATCATG TTCCCCTCAC AACACACAAC
    TTTGCTCGGA AGACGTTCCT GAAGCTTGCC TTCTGTGACA TCTGTCAGAA ATTCCTGCTC
    AATGGATTTC GATGTCAGAC TTGTGGCTAC AAATTTCATG AGCACTGTAG CACCAAAGTA
    CCTACTATGT GTGTGGACTG GAGTAACATC AGACAACTCT TTTCTCAGCA CAGATATTCT
    ACACCTCACG CCTTCACCTT TAACACCTCC AGTCCCTCAT CTGAAGGTTC CCTCTCCCAG
    AGGCAGAGGT CGACATCCAC ACCTAATGTC CACATGGTCA GCACCACCCT GCCTGTGGAC
    AGCAGGATGA TTGAGGATGC AATTCGAAGT CACAGCGAAT CAGCCTCACC TTCAGCCCTG
    TCCAGTAGCC CCAACAATCT GAGCCCAACA GGCTGGTCAC AGCCGAAAAC CCCCGTGCCA
    GCACAAAGAG AGCGGGCACC AGTATCTGGG ACCCAGGAGA AAAACAAAAT TAGGCCTCGT
    GGACAGAGAG ATTCAAGCTA TTATTGGGAA ATAGAAGCCA GTGAAGTGAT GCTGTCCACT
    CGGATTGGGT CAGGCTCTTT TGGAACTGTT TATAAGGGTA AATGGCACGG AGATGTTGCA
    GTAAAGATCC TAAAGGTTGT CGACCCAACC CCAGAGCAAT TCCAGGCCTT CAGGAATGAG
    GTGGCTGTTC TGCGCAAAAC ACGGCATGTG AACATTCTGC TTTTCATGGG GTACATGACA
    AAGGACAACC TGGCAATTGT GACCCAGTGG TGCGAGGGCA GCAGCCTCTA CAAACACCTG
    CATGTCCAGG AGACCAAGTT TCAGATGTTC CAGCTAATTG ACATTGCCCG GCAGACGGCT
    CAGGGAATGG ACTATTTGCA TGCAAAGAAC ATCATCCATA GAGACATGAA ATCCAACAAT
    ATATTTCTCC ATGAAGGCTT AACAGTGAAA ATTGGAGATT TTGGTTTGGC AACAGTAAAG
    TCACGCTGGA GTGGTTCTCA GCAGGTTGAA CAACCTACTG GCTCTGTCCT CTGGATGGCC
    CCAGAGGTGA TCCGAATGCA GGATAACAAC CCATTCAGTT TCCAGTCGGA TGTCTACTCC
    TATGGCATCG TATTGTATGA ACTGATGACG GGGGAGCTTC CTTATTCTCA CATCAACAAC
    CGAGATCAGA TCATCTTCAT GGTGGGCCGA GGATATGCCT CCCCAGATCT TAGTAAGCTA
    TATAAGAACT GCCCCAAAGC AATGAAGAGG CTGGTAGCTG ACTGTGTGAA GAAAGTAAAG
    GAAGAGAGGC CTCTTTTTCC CCAGATCCTG TCTTCCATTG AGCTGCTCCA ACACTCTCTA
    CCGAAGATCA ACCGGAGCGC TTCCGAGCCA TCCTTGCATC GGGCAGCCCA CACTGAGGAT
    ATCAATGCTT GCACGCTGAC CACGTCCCCG AGGCTGCCTG TCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu37401
    Clone ID Related Accession (Same CDS sequence) XM_011533975.2 , NM_001354691.2 , NM_001354691.1 , NM_001354692.2 , NM_001354692.1 , XM_005265358.4
    Accession Version NM_001354691.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1704bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-07
    Organism Homo sapiens(human)
    Product RAF proto-oncogene serine/threonine-protein kinase isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC026170.5 and AC018500.4. On Jun 2, 2019 this sequence version replaced NM_001354691.1. Transcript Variant: This variant (4), as well as variant 5, encodes isoform c. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.236853.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT TTCTTGCCGA 
    ACAAGCAAAG AACAGTGTAA AAAAGCACGC TTAGATTGGA ATACTGATGC TGCGTCTTTG
    ATTGGAGAAG AACTTCAAGT AGATTTCCTG GATCATGTTC CCCTCACAAC ACACAACTTT
    GCTCGGAAGA CGTTCCTGAA GCTTGCCTTC TGTGACATCT GTCAGAAATT CCTGCTCAAT
    GGATTTCGAT GTCAGACTTG TGGCTACAAA TTTCATGAGC ACTGTAGCAC CAAAGTACCT
    ACTATGTGTG TGGACTGGAG TAACATCAGA CAACTCTTAT TGTTTCCAAA TTCCACTATT
    GGTGATAGTG GAGTCCCAGC ACTACCTTCT TTGACTATGC GTCGTATGCG AGAGTCTGTT
    TCCAGGATGC CTGTTAGTTC TCAGCACAGA TATTCTACAC CTCACGCCTT CACCTTTAAC
    ACCTCCAGTC CCTCATCTGA AGGTTCCCTC TCCCAGAGGC AGAGGTCGAC ATCCACACCT
    AATGTCCACA TGGTCAGCAC CACCCTGCCT GTGGACAGCA GGATGATTGA GGATGCAATT
    CGAAGTCACA GCGAATCAGC CTCACCTTCA GCCCTGTCCA GTAGCCCCAA CAATCTGAGC
    CCAACAGGCT GGTCACAGCC GAAAACCCCC GTGCCAGCAC AAAGAGAGCG GGCACCAGTA
    TCTGGGACCC AGGAGAAAAA CAAAATTAGG CCTCGTGGAC AGAGAGATTC AAGCTATTAT
    TGGGAAATAG AAGCCAGTGA AGTGATGCTG TCCACTCGGA TTGGGTCAGG CTCTTTTGGA
    ACTGTTTATA AGGGTAAATG GCACGGAGAT GTTGCAGTAA AGATCCTAAA GGTTGTCGAC
    CCAACCCCAG AGCAATTCCA GGCCTTCAGG AATGAGGTGG CTGTTCTGCG CAAAACACGG
    CATGTGAACA TTCTGCTTTT CATGGGGTAC ATGACAAAGG ACAACCTGGC AATTGTGACC
    CAGTGGTGCG AGGGCAGCAG CCTCTACAAA CACCTGCATG TCCAGGAGAC CAAGTTTCAG
    ATGTTCCAGC TAATTGACAT TGCCCGGCAG ACGGCTCAGG GAATGGACTA TTTGCATGCA
    AAGAACATCA TCCATAGAGA CATGAAATCC AACAATATAT TTCTCCATGA AGGCTTAACA
    GTGAAAATTG GAGATTTTGG TTTGGCAACA GTAAAGTCAC GCTGGAGTGG TTCTCAGCAG
    GTTGAACAAC CTACTGGCTC TGTCCTCTGG ATGGCCCCAG AGGTGATCCG AATGCAGGAT
    AACAACCCAT TCAGTTTCCA GTCGGATGTC TACTCCTATG GCATCGTATT GTATGAACTG
    ATGACGGGGG AGCTTCCTTA TTCTCACATC AACAACCGAG ATCAGATCAT CTTCATGGTG
    GGCCGAGGAT ATGCCTCCCC AGATCTTAGT AAGCTATATA AGAACTGCCC CAAAGCAATG
    AAGAGGCTGG TAGCTGACTG TGTGAAGAAA GTAAAGGAAG AGAGGCCTCT TTTTCCCCAG
    ATCCTGTCTT CCATTGAGCT GCTCCAACAC TCTCTACCGA AGATCAACCG GAGCGCTTCC
    GAGCCATCCT TGCATCGGGC AGCCCACACT GAGGATATCA ATGCTTGCAC GCTGACCACG
    TCCCCGAGGC TGCCTGTCTT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001341620.1
    CDS555..2258
    Translation

    Target ORF information:

    RefSeq Version NM_001354691.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001354691.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT TTCTTGCCGA 
    ACAAGCAAAG AACAGTGTAA AAAAGCACGC TTAGATTGGA ATACTGATGC TGCGTCTTTG
    ATTGGAGAAG AACTTCAAGT AGATTTCCTG GATCATGTTC CCCTCACAAC ACACAACTTT
    GCTCGGAAGA CGTTCCTGAA GCTTGCCTTC TGTGACATCT GTCAGAAATT CCTGCTCAAT
    GGATTTCGAT GTCAGACTTG TGGCTACAAA TTTCATGAGC ACTGTAGCAC CAAAGTACCT
    ACTATGTGTG TGGACTGGAG TAACATCAGA CAACTCTTAT TGTTTCCAAA TTCCACTATT
    GGTGATAGTG GAGTCCCAGC ACTACCTTCT TTGACTATGC GTCGTATGCG AGAGTCTGTT
    TCCAGGATGC CTGTTAGTTC TCAGCACAGA TATTCTACAC CTCACGCCTT CACCTTTAAC
    ACCTCCAGTC CCTCATCTGA AGGTTCCCTC TCCCAGAGGC AGAGGTCGAC ATCCACACCT
    AATGTCCACA TGGTCAGCAC CACCCTGCCT GTGGACAGCA GGATGATTGA GGATGCAATT
    CGAAGTCACA GCGAATCAGC CTCACCTTCA GCCCTGTCCA GTAGCCCCAA CAATCTGAGC
    CCAACAGGCT GGTCACAGCC GAAAACCCCC GTGCCAGCAC AAAGAGAGCG GGCACCAGTA
    TCTGGGACCC AGGAGAAAAA CAAAATTAGG CCTCGTGGAC AGAGAGATTC AAGCTATTAT
    TGGGAAATAG AAGCCAGTGA AGTGATGCTG TCCACTCGGA TTGGGTCAGG CTCTTTTGGA
    ACTGTTTATA AGGGTAAATG GCACGGAGAT GTTGCAGTAA AGATCCTAAA GGTTGTCGAC
    CCAACCCCAG AGCAATTCCA GGCCTTCAGG AATGAGGTGG CTGTTCTGCG CAAAACACGG
    CATGTGAACA TTCTGCTTTT CATGGGGTAC ATGACAAAGG ACAACCTGGC AATTGTGACC
    CAGTGGTGCG AGGGCAGCAG CCTCTACAAA CACCTGCATG TCCAGGAGAC CAAGTTTCAG
    ATGTTCCAGC TAATTGACAT TGCCCGGCAG ACGGCTCAGG GAATGGACTA TTTGCATGCA
    AAGAACATCA TCCATAGAGA CATGAAATCC AACAATATAT TTCTCCATGA AGGCTTAACA
    GTGAAAATTG GAGATTTTGG TTTGGCAACA GTAAAGTCAC GCTGGAGTGG TTCTCAGCAG
    GTTGAACAAC CTACTGGCTC TGTCCTCTGG ATGGCCCCAG AGGTGATCCG AATGCAGGAT
    AACAACCCAT TCAGTTTCCA GTCGGATGTC TACTCCTATG GCATCGTATT GTATGAACTG
    ATGACGGGGG AGCTTCCTTA TTCTCACATC AACAACCGAG ATCAGATCAT CTTCATGGTG
    GGCCGAGGAT ATGCCTCCCC AGATCTTAGT AAGCTATATA AGAACTGCCC CAAAGCAATG
    AAGAGGCTGG TAGCTGACTG TGTGAAGAAA GTAAAGGAAG AGAGGCCTCT TTTTCCCCAG
    ATCCTGTCTT CCATTGAGCT GCTCCAACAC TCTCTACCGA AGATCAACCG GAGCGCTTCC
    GAGCCATCCT TGCATCGGGC AGCCCACACT GAGGATATCA ATGCTTGCAC GCTGACCACG
    TCCCCGAGGC TGCCTGTCTT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu37401
    Clone ID Related Accession (Same CDS sequence) XM_011533975.2 , NM_001354691.2 , NM_001354691.1 , NM_001354692.2 , NM_001354692.1 , XM_005265358.4
    Accession Version NM_001354691.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1704bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product RAF proto-oncogene serine/threonine-protein kinase isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC026170.5 and AC018500.4. On Aug 18, 2017 this sequence version replaced XM_011533975.2. Transcript Variant: This variant (4), as well as variant 5, encodes isoform c. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.236853.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT TTCTTGCCGA 
    ACAAGCAAAG AACAGTGTAA AAAAGCACGC TTAGATTGGA ATACTGATGC TGCGTCTTTG
    ATTGGAGAAG AACTTCAAGT AGATTTCCTG GATCATGTTC CCCTCACAAC ACACAACTTT
    GCTCGGAAGA CGTTCCTGAA GCTTGCCTTC TGTGACATCT GTCAGAAATT CCTGCTCAAT
    GGATTTCGAT GTCAGACTTG TGGCTACAAA TTTCATGAGC ACTGTAGCAC CAAAGTACCT
    ACTATGTGTG TGGACTGGAG TAACATCAGA CAACTCTTAT TGTTTCCAAA TTCCACTATT
    GGTGATAGTG GAGTCCCAGC ACTACCTTCT TTGACTATGC GTCGTATGCG AGAGTCTGTT
    TCCAGGATGC CTGTTAGTTC TCAGCACAGA TATTCTACAC CTCACGCCTT CACCTTTAAC
    ACCTCCAGTC CCTCATCTGA AGGTTCCCTC TCCCAGAGGC AGAGGTCGAC ATCCACACCT
    AATGTCCACA TGGTCAGCAC CACCCTGCCT GTGGACAGCA GGATGATTGA GGATGCAATT
    CGAAGTCACA GCGAATCAGC CTCACCTTCA GCCCTGTCCA GTAGCCCCAA CAATCTGAGC
    CCAACAGGCT GGTCACAGCC GAAAACCCCC GTGCCAGCAC AAAGAGAGCG GGCACCAGTA
    TCTGGGACCC AGGAGAAAAA CAAAATTAGG CCTCGTGGAC AGAGAGATTC AAGCTATTAT
    TGGGAAATAG AAGCCAGTGA AGTGATGCTG TCCACTCGGA TTGGGTCAGG CTCTTTTGGA
    ACTGTTTATA AGGGTAAATG GCACGGAGAT GTTGCAGTAA AGATCCTAAA GGTTGTCGAC
    CCAACCCCAG AGCAATTCCA GGCCTTCAGG AATGAGGTGG CTGTTCTGCG CAAAACACGG
    CATGTGAACA TTCTGCTTTT CATGGGGTAC ATGACAAAGG ACAACCTGGC AATTGTGACC
    CAGTGGTGCG AGGGCAGCAG CCTCTACAAA CACCTGCATG TCCAGGAGAC CAAGTTTCAG
    ATGTTCCAGC TAATTGACAT TGCCCGGCAG ACGGCTCAGG GAATGGACTA TTTGCATGCA
    AAGAACATCA TCCATAGAGA CATGAAATCC AACAATATAT TTCTCCATGA AGGCTTAACA
    GTGAAAATTG GAGATTTTGG TTTGGCAACA GTAAAGTCAC GCTGGAGTGG TTCTCAGCAG
    GTTGAACAAC CTACTGGCTC TGTCCTCTGG ATGGCCCCAG AGGTGATCCG AATGCAGGAT
    AACAACCCAT TCAGTTTCCA GTCGGATGTC TACTCCTATG GCATCGTATT GTATGAACTG
    ATGACGGGGG AGCTTCCTTA TTCTCACATC AACAACCGAG ATCAGATCAT CTTCATGGTG
    GGCCGAGGAT ATGCCTCCCC AGATCTTAGT AAGCTATATA AGAACTGCCC CAAAGCAATG
    AAGAGGCTGG TAGCTGACTG TGTGAAGAAA GTAAAGGAAG AGAGGCCTCT TTTTCCCCAG
    ATCCTGTCTT CCATTGAGCT GCTCCAACAC TCTCTACCGA AGATCAACCG GAGCGCTTCC
    GAGCCATCCT TGCATCGGGC AGCCCACACT GAGGATATCA ATGCTTGCAC GCTGACCACG
    TCCCCGAGGC TGCCTGTCTT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001341620.1
    CDS639..2342
    Translation

    Target ORF information:

    RefSeq Version NM_001354691.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001354691.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT TTCTTGCCGA 
    ACAAGCAAAG AACAGTGTAA AAAAGCACGC TTAGATTGGA ATACTGATGC TGCGTCTTTG
    ATTGGAGAAG AACTTCAAGT AGATTTCCTG GATCATGTTC CCCTCACAAC ACACAACTTT
    GCTCGGAAGA CGTTCCTGAA GCTTGCCTTC TGTGACATCT GTCAGAAATT CCTGCTCAAT
    GGATTTCGAT GTCAGACTTG TGGCTACAAA TTTCATGAGC ACTGTAGCAC CAAAGTACCT
    ACTATGTGTG TGGACTGGAG TAACATCAGA CAACTCTTAT TGTTTCCAAA TTCCACTATT
    GGTGATAGTG GAGTCCCAGC ACTACCTTCT TTGACTATGC GTCGTATGCG AGAGTCTGTT
    TCCAGGATGC CTGTTAGTTC TCAGCACAGA TATTCTACAC CTCACGCCTT CACCTTTAAC
    ACCTCCAGTC CCTCATCTGA AGGTTCCCTC TCCCAGAGGC AGAGGTCGAC ATCCACACCT
    AATGTCCACA TGGTCAGCAC CACCCTGCCT GTGGACAGCA GGATGATTGA GGATGCAATT
    CGAAGTCACA GCGAATCAGC CTCACCTTCA GCCCTGTCCA GTAGCCCCAA CAATCTGAGC
    CCAACAGGCT GGTCACAGCC GAAAACCCCC GTGCCAGCAC AAAGAGAGCG GGCACCAGTA
    TCTGGGACCC AGGAGAAAAA CAAAATTAGG CCTCGTGGAC AGAGAGATTC AAGCTATTAT
    TGGGAAATAG AAGCCAGTGA AGTGATGCTG TCCACTCGGA TTGGGTCAGG CTCTTTTGGA
    ACTGTTTATA AGGGTAAATG GCACGGAGAT GTTGCAGTAA AGATCCTAAA GGTTGTCGAC
    CCAACCCCAG AGCAATTCCA GGCCTTCAGG AATGAGGTGG CTGTTCTGCG CAAAACACGG
    CATGTGAACA TTCTGCTTTT CATGGGGTAC ATGACAAAGG ACAACCTGGC AATTGTGACC
    CAGTGGTGCG AGGGCAGCAG CCTCTACAAA CACCTGCATG TCCAGGAGAC CAAGTTTCAG
    ATGTTCCAGC TAATTGACAT TGCCCGGCAG ACGGCTCAGG GAATGGACTA TTTGCATGCA
    AAGAACATCA TCCATAGAGA CATGAAATCC AACAATATAT TTCTCCATGA AGGCTTAACA
    GTGAAAATTG GAGATTTTGG TTTGGCAACA GTAAAGTCAC GCTGGAGTGG TTCTCAGCAG
    GTTGAACAAC CTACTGGCTC TGTCCTCTGG ATGGCCCCAG AGGTGATCCG AATGCAGGAT
    AACAACCCAT TCAGTTTCCA GTCGGATGTC TACTCCTATG GCATCGTATT GTATGAACTG
    ATGACGGGGG AGCTTCCTTA TTCTCACATC AACAACCGAG ATCAGATCAT CTTCATGGTG
    GGCCGAGGAT ATGCCTCCCC AGATCTTAGT AAGCTATATA AGAACTGCCC CAAAGCAATG
    AAGAGGCTGG TAGCTGACTG TGTGAAGAAA GTAAAGGAAG AGAGGCCTCT TTTTCCCCAG
    ATCCTGTCTT CCATTGAGCT GCTCCAACAC TCTCTACCGA AGATCAACCG GAGCGCTTCC
    GAGCCATCCT TGCATCGGGC AGCCCACACT GAGGATATCA ATGCTTGCAC GCTGACCACG
    TCCCCGAGGC TGCCTGTCTT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu37401
    Clone ID Related Accession (Same CDS sequence) XM_011533975.2 , NM_001354691.2 , NM_001354691.1 , NM_001354692.2 , NM_001354692.1 , XM_005265358.4
    Accession Version NM_001354692.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1704bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-07
    Organism Homo sapiens(human)
    Product RAF proto-oncogene serine/threonine-protein kinase isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC026170.5 and AC018500.4. On Jun 2, 2019 this sequence version replaced NM_001354692.1. Transcript Variant: This variant (5), as well as variant 4, encodes isoform c. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.98132.1, SRR1660809.54041.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT TTCTTGCCGA 
    ACAAGCAAAG AACAGTGTAA AAAAGCACGC TTAGATTGGA ATACTGATGC TGCGTCTTTG
    ATTGGAGAAG AACTTCAAGT AGATTTCCTG GATCATGTTC CCCTCACAAC ACACAACTTT
    GCTCGGAAGA CGTTCCTGAA GCTTGCCTTC TGTGACATCT GTCAGAAATT CCTGCTCAAT
    GGATTTCGAT GTCAGACTTG TGGCTACAAA TTTCATGAGC ACTGTAGCAC CAAAGTACCT
    ACTATGTGTG TGGACTGGAG TAACATCAGA CAACTCTTAT TGTTTCCAAA TTCCACTATT
    GGTGATAGTG GAGTCCCAGC ACTACCTTCT TTGACTATGC GTCGTATGCG AGAGTCTGTT
    TCCAGGATGC CTGTTAGTTC TCAGCACAGA TATTCTACAC CTCACGCCTT CACCTTTAAC
    ACCTCCAGTC CCTCATCTGA AGGTTCCCTC TCCCAGAGGC AGAGGTCGAC ATCCACACCT
    AATGTCCACA TGGTCAGCAC CACCCTGCCT GTGGACAGCA GGATGATTGA GGATGCAATT
    CGAAGTCACA GCGAATCAGC CTCACCTTCA GCCCTGTCCA GTAGCCCCAA CAATCTGAGC
    CCAACAGGCT GGTCACAGCC GAAAACCCCC GTGCCAGCAC AAAGAGAGCG GGCACCAGTA
    TCTGGGACCC AGGAGAAAAA CAAAATTAGG CCTCGTGGAC AGAGAGATTC AAGCTATTAT
    TGGGAAATAG AAGCCAGTGA AGTGATGCTG TCCACTCGGA TTGGGTCAGG CTCTTTTGGA
    ACTGTTTATA AGGGTAAATG GCACGGAGAT GTTGCAGTAA AGATCCTAAA GGTTGTCGAC
    CCAACCCCAG AGCAATTCCA GGCCTTCAGG AATGAGGTGG CTGTTCTGCG CAAAACACGG
    CATGTGAACA TTCTGCTTTT CATGGGGTAC ATGACAAAGG ACAACCTGGC AATTGTGACC
    CAGTGGTGCG AGGGCAGCAG CCTCTACAAA CACCTGCATG TCCAGGAGAC CAAGTTTCAG
    ATGTTCCAGC TAATTGACAT TGCCCGGCAG ACGGCTCAGG GAATGGACTA TTTGCATGCA
    AAGAACATCA TCCATAGAGA CATGAAATCC AACAATATAT TTCTCCATGA AGGCTTAACA
    GTGAAAATTG GAGATTTTGG TTTGGCAACA GTAAAGTCAC GCTGGAGTGG TTCTCAGCAG
    GTTGAACAAC CTACTGGCTC TGTCCTCTGG ATGGCCCCAG AGGTGATCCG AATGCAGGAT
    AACAACCCAT TCAGTTTCCA GTCGGATGTC TACTCCTATG GCATCGTATT GTATGAACTG
    ATGACGGGGG AGCTTCCTTA TTCTCACATC AACAACCGAG ATCAGATCAT CTTCATGGTG
    GGCCGAGGAT ATGCCTCCCC AGATCTTAGT AAGCTATATA AGAACTGCCC CAAAGCAATG
    AAGAGGCTGG TAGCTGACTG TGTGAAGAAA GTAAAGGAAG AGAGGCCTCT TTTTCCCCAG
    ATCCTGTCTT CCATTGAGCT GCTCCAACAC TCTCTACCGA AGATCAACCG GAGCGCTTCC
    GAGCCATCCT TGCATCGGGC AGCCCACACT GAGGATATCA ATGCTTGCAC GCTGACCACG
    TCCCCGAGGC TGCCTGTCTT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001341621.1
    CDS462..2165
    Translation

    Target ORF information:

    RefSeq Version NM_001354692.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001354692.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT TTCTTGCCGA 
    ACAAGCAAAG AACAGTGTAA AAAAGCACGC TTAGATTGGA ATACTGATGC TGCGTCTTTG
    ATTGGAGAAG AACTTCAAGT AGATTTCCTG GATCATGTTC CCCTCACAAC ACACAACTTT
    GCTCGGAAGA CGTTCCTGAA GCTTGCCTTC TGTGACATCT GTCAGAAATT CCTGCTCAAT
    GGATTTCGAT GTCAGACTTG TGGCTACAAA TTTCATGAGC ACTGTAGCAC CAAAGTACCT
    ACTATGTGTG TGGACTGGAG TAACATCAGA CAACTCTTAT TGTTTCCAAA TTCCACTATT
    GGTGATAGTG GAGTCCCAGC ACTACCTTCT TTGACTATGC GTCGTATGCG AGAGTCTGTT
    TCCAGGATGC CTGTTAGTTC TCAGCACAGA TATTCTACAC CTCACGCCTT CACCTTTAAC
    ACCTCCAGTC CCTCATCTGA AGGTTCCCTC TCCCAGAGGC AGAGGTCGAC ATCCACACCT
    AATGTCCACA TGGTCAGCAC CACCCTGCCT GTGGACAGCA GGATGATTGA GGATGCAATT
    CGAAGTCACA GCGAATCAGC CTCACCTTCA GCCCTGTCCA GTAGCCCCAA CAATCTGAGC
    CCAACAGGCT GGTCACAGCC GAAAACCCCC GTGCCAGCAC AAAGAGAGCG GGCACCAGTA
    TCTGGGACCC AGGAGAAAAA CAAAATTAGG CCTCGTGGAC AGAGAGATTC AAGCTATTAT
    TGGGAAATAG AAGCCAGTGA AGTGATGCTG TCCACTCGGA TTGGGTCAGG CTCTTTTGGA
    ACTGTTTATA AGGGTAAATG GCACGGAGAT GTTGCAGTAA AGATCCTAAA GGTTGTCGAC
    CCAACCCCAG AGCAATTCCA GGCCTTCAGG AATGAGGTGG CTGTTCTGCG CAAAACACGG
    CATGTGAACA TTCTGCTTTT CATGGGGTAC ATGACAAAGG ACAACCTGGC AATTGTGACC
    CAGTGGTGCG AGGGCAGCAG CCTCTACAAA CACCTGCATG TCCAGGAGAC CAAGTTTCAG
    ATGTTCCAGC TAATTGACAT TGCCCGGCAG ACGGCTCAGG GAATGGACTA TTTGCATGCA
    AAGAACATCA TCCATAGAGA CATGAAATCC AACAATATAT TTCTCCATGA AGGCTTAACA
    GTGAAAATTG GAGATTTTGG TTTGGCAACA GTAAAGTCAC GCTGGAGTGG TTCTCAGCAG
    GTTGAACAAC CTACTGGCTC TGTCCTCTGG ATGGCCCCAG AGGTGATCCG AATGCAGGAT
    AACAACCCAT TCAGTTTCCA GTCGGATGTC TACTCCTATG GCATCGTATT GTATGAACTG
    ATGACGGGGG AGCTTCCTTA TTCTCACATC AACAACCGAG ATCAGATCAT CTTCATGGTG
    GGCCGAGGAT ATGCCTCCCC AGATCTTAGT AAGCTATATA AGAACTGCCC CAAAGCAATG
    AAGAGGCTGG TAGCTGACTG TGTGAAGAAA GTAAAGGAAG AGAGGCCTCT TTTTCCCCAG
    ATCCTGTCTT CCATTGAGCT GCTCCAACAC TCTCTACCGA AGATCAACCG GAGCGCTTCC
    GAGCCATCCT TGCATCGGGC AGCCCACACT GAGGATATCA ATGCTTGCAC GCTGACCACG
    TCCCCGAGGC TGCCTGTCTT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu37401
    Clone ID Related Accession (Same CDS sequence) XM_011533975.2 , NM_001354691.2 , NM_001354691.1 , NM_001354692.2 , NM_001354692.1 , XM_005265358.4
    Accession Version NM_001354692.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1704bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product RAF proto-oncogene serine/threonine-protein kinase isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC026170.5 and AC018500.4. On Aug 29, 2017 this sequence version replaced XM_005265358.4. Transcript Variant: This variant (5), as well as variant 4, encodes isoform c. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.54041.1, SRR1660805.179515.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT TTCTTGCCGA 
    ACAAGCAAAG AACAGTGTAA AAAAGCACGC TTAGATTGGA ATACTGATGC TGCGTCTTTG
    ATTGGAGAAG AACTTCAAGT AGATTTCCTG GATCATGTTC CCCTCACAAC ACACAACTTT
    GCTCGGAAGA CGTTCCTGAA GCTTGCCTTC TGTGACATCT GTCAGAAATT CCTGCTCAAT
    GGATTTCGAT GTCAGACTTG TGGCTACAAA TTTCATGAGC ACTGTAGCAC CAAAGTACCT
    ACTATGTGTG TGGACTGGAG TAACATCAGA CAACTCTTAT TGTTTCCAAA TTCCACTATT
    GGTGATAGTG GAGTCCCAGC ACTACCTTCT TTGACTATGC GTCGTATGCG AGAGTCTGTT
    TCCAGGATGC CTGTTAGTTC TCAGCACAGA TATTCTACAC CTCACGCCTT CACCTTTAAC
    ACCTCCAGTC CCTCATCTGA AGGTTCCCTC TCCCAGAGGC AGAGGTCGAC ATCCACACCT
    AATGTCCACA TGGTCAGCAC CACCCTGCCT GTGGACAGCA GGATGATTGA GGATGCAATT
    CGAAGTCACA GCGAATCAGC CTCACCTTCA GCCCTGTCCA GTAGCCCCAA CAATCTGAGC
    CCAACAGGCT GGTCACAGCC GAAAACCCCC GTGCCAGCAC AAAGAGAGCG GGCACCAGTA
    TCTGGGACCC AGGAGAAAAA CAAAATTAGG CCTCGTGGAC AGAGAGATTC AAGCTATTAT
    TGGGAAATAG AAGCCAGTGA AGTGATGCTG TCCACTCGGA TTGGGTCAGG CTCTTTTGGA
    ACTGTTTATA AGGGTAAATG GCACGGAGAT GTTGCAGTAA AGATCCTAAA GGTTGTCGAC
    CCAACCCCAG AGCAATTCCA GGCCTTCAGG AATGAGGTGG CTGTTCTGCG CAAAACACGG
    CATGTGAACA TTCTGCTTTT CATGGGGTAC ATGACAAAGG ACAACCTGGC AATTGTGACC
    CAGTGGTGCG AGGGCAGCAG CCTCTACAAA CACCTGCATG TCCAGGAGAC CAAGTTTCAG
    ATGTTCCAGC TAATTGACAT TGCCCGGCAG ACGGCTCAGG GAATGGACTA TTTGCATGCA
    AAGAACATCA TCCATAGAGA CATGAAATCC AACAATATAT TTCTCCATGA AGGCTTAACA
    GTGAAAATTG GAGATTTTGG TTTGGCAACA GTAAAGTCAC GCTGGAGTGG TTCTCAGCAG
    GTTGAACAAC CTACTGGCTC TGTCCTCTGG ATGGCCCCAG AGGTGATCCG AATGCAGGAT
    AACAACCCAT TCAGTTTCCA GTCGGATGTC TACTCCTATG GCATCGTATT GTATGAACTG
    ATGACGGGGG AGCTTCCTTA TTCTCACATC AACAACCGAG ATCAGATCAT CTTCATGGTG
    GGCCGAGGAT ATGCCTCCCC AGATCTTAGT AAGCTATATA AGAACTGCCC CAAAGCAATG
    AAGAGGCTGG TAGCTGACTG TGTGAAGAAA GTAAAGGAAG AGAGGCCTCT TTTTCCCCAG
    ATCCTGTCTT CCATTGAGCT GCTCCAACAC TCTCTACCGA AGATCAACCG GAGCGCTTCC
    GAGCCATCCT TGCATCGGGC AGCCCACACT GAGGATATCA ATGCTTGCAC GCTGACCACG
    TCCCCGAGGC TGCCTGTCTT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001341621.1
    CDS546..2249
    Translation

    Target ORF information:

    RefSeq Version NM_001354692.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001354692.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT TTCTTGCCGA 
    ACAAGCAAAG AACAGTGTAA AAAAGCACGC TTAGATTGGA ATACTGATGC TGCGTCTTTG
    ATTGGAGAAG AACTTCAAGT AGATTTCCTG GATCATGTTC CCCTCACAAC ACACAACTTT
    GCTCGGAAGA CGTTCCTGAA GCTTGCCTTC TGTGACATCT GTCAGAAATT CCTGCTCAAT
    GGATTTCGAT GTCAGACTTG TGGCTACAAA TTTCATGAGC ACTGTAGCAC CAAAGTACCT
    ACTATGTGTG TGGACTGGAG TAACATCAGA CAACTCTTAT TGTTTCCAAA TTCCACTATT
    GGTGATAGTG GAGTCCCAGC ACTACCTTCT TTGACTATGC GTCGTATGCG AGAGTCTGTT
    TCCAGGATGC CTGTTAGTTC TCAGCACAGA TATTCTACAC CTCACGCCTT CACCTTTAAC
    ACCTCCAGTC CCTCATCTGA AGGTTCCCTC TCCCAGAGGC AGAGGTCGAC ATCCACACCT
    AATGTCCACA TGGTCAGCAC CACCCTGCCT GTGGACAGCA GGATGATTGA GGATGCAATT
    CGAAGTCACA GCGAATCAGC CTCACCTTCA GCCCTGTCCA GTAGCCCCAA CAATCTGAGC
    CCAACAGGCT GGTCACAGCC GAAAACCCCC GTGCCAGCAC AAAGAGAGCG GGCACCAGTA
    TCTGGGACCC AGGAGAAAAA CAAAATTAGG CCTCGTGGAC AGAGAGATTC AAGCTATTAT
    TGGGAAATAG AAGCCAGTGA AGTGATGCTG TCCACTCGGA TTGGGTCAGG CTCTTTTGGA
    ACTGTTTATA AGGGTAAATG GCACGGAGAT GTTGCAGTAA AGATCCTAAA GGTTGTCGAC
    CCAACCCCAG AGCAATTCCA GGCCTTCAGG AATGAGGTGG CTGTTCTGCG CAAAACACGG
    CATGTGAACA TTCTGCTTTT CATGGGGTAC ATGACAAAGG ACAACCTGGC AATTGTGACC
    CAGTGGTGCG AGGGCAGCAG CCTCTACAAA CACCTGCATG TCCAGGAGAC CAAGTTTCAG
    ATGTTCCAGC TAATTGACAT TGCCCGGCAG ACGGCTCAGG GAATGGACTA TTTGCATGCA
    AAGAACATCA TCCATAGAGA CATGAAATCC AACAATATAT TTCTCCATGA AGGCTTAACA
    GTGAAAATTG GAGATTTTGG TTTGGCAACA GTAAAGTCAC GCTGGAGTGG TTCTCAGCAG
    GTTGAACAAC CTACTGGCTC TGTCCTCTGG ATGGCCCCAG AGGTGATCCG AATGCAGGAT
    AACAACCCAT TCAGTTTCCA GTCGGATGTC TACTCCTATG GCATCGTATT GTATGAACTG
    ATGACGGGGG AGCTTCCTTA TTCTCACATC AACAACCGAG ATCAGATCAT CTTCATGGTG
    GGCCGAGGAT ATGCCTCCCC AGATCTTAGT AAGCTATATA AGAACTGCCC CAAAGCAATG
    AAGAGGCTGG TAGCTGACTG TGTGAAGAAA GTAAAGGAAG AGAGGCCTCT TTTTCCCCAG
    ATCCTGTCTT CCATTGAGCT GCTCCAACAC TCTCTACCGA AGATCAACCG GAGCGCTTCC
    GAGCCATCCT TGCATCGGGC AGCCCACACT GAGGATATCA ATGCTTGCAC GCTGACCACG
    TCCCCGAGGC TGCCTGTCTT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu37402
    Clone ID Related Accession (Same CDS sequence) NM_001354695.1 , XM_005265359.4 , NM_001354695.2
    Accession Version NM_001354695.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1605bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product RAF proto-oncogene serine/threonine-protein kinase isoform f
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC026170.5 and AC018500.4. On Aug 29, 2017 this sequence version replaced XM_005265359.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.191340.1, SRR1803617.31217.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT TTCTTGCCGA 
    ACAAGCAAAG AACAGTGTAA AAAAGCACGC TTAGATTGGA ATACTGATGC TGCGTCTTTG
    ATTGGAGAAG AACTTCAAGT AGATTTCCTG GATCATGTTC CCCTCACAAC ACACAACTTT
    GCTCGGAAGA CGTTCCTGAA GCTTGCCTTC TGTGACATCT GTCAGAAATT CCTGCTCAAT
    GGATTTCGAT GTCAGACTTG TGGCTACAAA TTTCATGAGC ACTGTAGCAC CAAAGTACCT
    ACTATGTGTG TGGACTGGAG TAACATCAGA CAACTCTTTT CTCAGCACAG ATATTCTACA
    CCTCACGCCT TCACCTTTAA CACCTCCAGT CCCTCATCTG AAGGTTCCCT CTCCCAGAGG
    CAGAGGTCGA CATCCACACC TAATGTCCAC ATGGTCAGCA CCACCCTGCC TGTGGACAGC
    AGGATGATTG AGGATGCAAT TCGAAGTCAC AGCGAATCAG CCTCACCTTC AGCCCTGTCC
    AGTAGCCCCA ACAATCTGAG CCCAACAGGC TGGTCACAGC CGAAAACCCC CGTGCCAGCA
    CAAAGAGAGC GGGCACCAGT ATCTGGGACC CAGGAGAAAA ACAAAATTAG GCCTCGTGGA
    CAGAGAGATT CAAGCTATTA TTGGGAAATA GAAGCCAGTG AAGTGATGCT GTCCACTCGG
    ATTGGGTCAG GCTCTTTTGG AACTGTTTAT AAGGGTAAAT GGCACGGAGA TGTTGCAGTA
    AAGATCCTAA AGGTTGTCGA CCCAACCCCA GAGCAATTCC AGGCCTTCAG GAATGAGGTG
    GCTGTTCTGC GCAAAACACG GCATGTGAAC ATTCTGCTTT TCATGGGGTA CATGACAAAG
    GACAACCTGG CAATTGTGAC CCAGTGGTGC GAGGGCAGCA GCCTCTACAA ACACCTGCAT
    GTCCAGGAGA CCAAGTTTCA GATGTTCCAG CTAATTGACA TTGCCCGGCA GACGGCTCAG
    GGAATGGACT ATTTGCATGC AAAGAACATC ATCCATAGAG ACATGAAATC CAACAATATA
    TTTCTCCATG AAGGCTTAAC AGTGAAAATT GGAGATTTTG GTTTGGCAAC AGTAAAGTCA
    CGCTGGAGTG GTTCTCAGCA GGTTGAACAA CCTACTGGCT CTGTCCTCTG GATGGCCCCA
    GAGGTGATCC GAATGCAGGA TAACAACCCA TTCAGTTTCC AGTCGGATGT CTACTCCTAT
    GGCATCGTAT TGTATGAACT GATGACGGGG GAGCTTCCTT ATTCTCACAT CAACAACCGA
    GATCAGATCA TCTTCATGGT GGGCCGAGGA TATGCCTCCC CAGATCTTAG TAAGCTATAT
    AAGAACTGCC CCAAAGCAAT GAAGAGGCTG GTAGCTGACT GTGTGAAGAA AGTAAAGGAA
    GAGAGGCCTC TTTTTCCCCA GATCCTGTCT TCCATTGAGC TGCTCCAACA CTCTCTACCG
    AAGATCAACC GGAGCGCTTC CGAGCCATCC TTGCATCGGG CAGCCCACAC TGAGGATATC
    AATGCTTGCA CGCTGACCAC GTCCCCGAGG CTGCCTGTCT TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001341624.1
    CDS546..2150
    Translation

    Target ORF information:

    RefSeq Version NM_001354695.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001354695.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT TTCTTGCCGA 
    ACAAGCAAAG AACAGTGTAA AAAAGCACGC TTAGATTGGA ATACTGATGC TGCGTCTTTG
    ATTGGAGAAG AACTTCAAGT AGATTTCCTG GATCATGTTC CCCTCACAAC ACACAACTTT
    GCTCGGAAGA CGTTCCTGAA GCTTGCCTTC TGTGACATCT GTCAGAAATT CCTGCTCAAT
    GGATTTCGAT GTCAGACTTG TGGCTACAAA TTTCATGAGC ACTGTAGCAC CAAAGTACCT
    ACTATGTGTG TGGACTGGAG TAACATCAGA CAACTCTTTT CTCAGCACAG ATATTCTACA
    CCTCACGCCT TCACCTTTAA CACCTCCAGT CCCTCATCTG AAGGTTCCCT CTCCCAGAGG
    CAGAGGTCGA CATCCACACC TAATGTCCAC ATGGTCAGCA CCACCCTGCC TGTGGACAGC
    AGGATGATTG AGGATGCAAT TCGAAGTCAC AGCGAATCAG CCTCACCTTC AGCCCTGTCC
    AGTAGCCCCA ACAATCTGAG CCCAACAGGC TGGTCACAGC CGAAAACCCC CGTGCCAGCA
    CAAAGAGAGC GGGCACCAGT ATCTGGGACC CAGGAGAAAA ACAAAATTAG GCCTCGTGGA
    CAGAGAGATT CAAGCTATTA TTGGGAAATA GAAGCCAGTG AAGTGATGCT GTCCACTCGG
    ATTGGGTCAG GCTCTTTTGG AACTGTTTAT AAGGGTAAAT GGCACGGAGA TGTTGCAGTA
    AAGATCCTAA AGGTTGTCGA CCCAACCCCA GAGCAATTCC AGGCCTTCAG GAATGAGGTG
    GCTGTTCTGC GCAAAACACG GCATGTGAAC ATTCTGCTTT TCATGGGGTA CATGACAAAG
    GACAACCTGG CAATTGTGAC CCAGTGGTGC GAGGGCAGCA GCCTCTACAA ACACCTGCAT
    GTCCAGGAGA CCAAGTTTCA GATGTTCCAG CTAATTGACA TTGCCCGGCA GACGGCTCAG
    GGAATGGACT ATTTGCATGC AAAGAACATC ATCCATAGAG ACATGAAATC CAACAATATA
    TTTCTCCATG AAGGCTTAAC AGTGAAAATT GGAGATTTTG GTTTGGCAAC AGTAAAGTCA
    CGCTGGAGTG GTTCTCAGCA GGTTGAACAA CCTACTGGCT CTGTCCTCTG GATGGCCCCA
    GAGGTGATCC GAATGCAGGA TAACAACCCA TTCAGTTTCC AGTCGGATGT CTACTCCTAT
    GGCATCGTAT TGTATGAACT GATGACGGGG GAGCTTCCTT ATTCTCACAT CAACAACCGA
    GATCAGATCA TCTTCATGGT GGGCCGAGGA TATGCCTCCC CAGATCTTAG TAAGCTATAT
    AAGAACTGCC CCAAAGCAAT GAAGAGGCTG GTAGCTGACT GTGTGAAGAA AGTAAAGGAA
    GAGAGGCCTC TTTTTCCCCA GATCCTGTCT TCCATTGAGC TGCTCCAACA CTCTCTACCG
    AAGATCAACC GGAGCGCTTC CGAGCCATCC TTGCATCGGG CAGCCCACAC TGAGGATATC
    AATGCTTGCA CGCTGACCAC GTCCCCGAGG CTGCCTGTCT TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu37402
    Clone ID Related Accession (Same CDS sequence) NM_001354695.1 , XM_005265359.4 , NM_001354695.2
    Accession Version NM_001354695.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1605bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-07
    Organism Homo sapiens(human)
    Product RAF proto-oncogene serine/threonine-protein kinase isoform f
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC026170.5 and AC018500.4. On Jun 2, 2019 this sequence version replaced NM_001354695.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.191340.1, SRR1803615.247900.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT TTCTTGCCGA 
    ACAAGCAAAG AACAGTGTAA AAAAGCACGC TTAGATTGGA ATACTGATGC TGCGTCTTTG
    ATTGGAGAAG AACTTCAAGT AGATTTCCTG GATCATGTTC CCCTCACAAC ACACAACTTT
    GCTCGGAAGA CGTTCCTGAA GCTTGCCTTC TGTGACATCT GTCAGAAATT CCTGCTCAAT
    GGATTTCGAT GTCAGACTTG TGGCTACAAA TTTCATGAGC ACTGTAGCAC CAAAGTACCT
    ACTATGTGTG TGGACTGGAG TAACATCAGA CAACTCTTTT CTCAGCACAG ATATTCTACA
    CCTCACGCCT TCACCTTTAA CACCTCCAGT CCCTCATCTG AAGGTTCCCT CTCCCAGAGG
    CAGAGGTCGA CATCCACACC TAATGTCCAC ATGGTCAGCA CCACCCTGCC TGTGGACAGC
    AGGATGATTG AGGATGCAAT TCGAAGTCAC AGCGAATCAG CCTCACCTTC AGCCCTGTCC
    AGTAGCCCCA ACAATCTGAG CCCAACAGGC TGGTCACAGC CGAAAACCCC CGTGCCAGCA
    CAAAGAGAGC GGGCACCAGT ATCTGGGACC CAGGAGAAAA ACAAAATTAG GCCTCGTGGA
    CAGAGAGATT CAAGCTATTA TTGGGAAATA GAAGCCAGTG AAGTGATGCT GTCCACTCGG
    ATTGGGTCAG GCTCTTTTGG AACTGTTTAT AAGGGTAAAT GGCACGGAGA TGTTGCAGTA
    AAGATCCTAA AGGTTGTCGA CCCAACCCCA GAGCAATTCC AGGCCTTCAG GAATGAGGTG
    GCTGTTCTGC GCAAAACACG GCATGTGAAC ATTCTGCTTT TCATGGGGTA CATGACAAAG
    GACAACCTGG CAATTGTGAC CCAGTGGTGC GAGGGCAGCA GCCTCTACAA ACACCTGCAT
    GTCCAGGAGA CCAAGTTTCA GATGTTCCAG CTAATTGACA TTGCCCGGCA GACGGCTCAG
    GGAATGGACT ATTTGCATGC AAAGAACATC ATCCATAGAG ACATGAAATC CAACAATATA
    TTTCTCCATG AAGGCTTAAC AGTGAAAATT GGAGATTTTG GTTTGGCAAC AGTAAAGTCA
    CGCTGGAGTG GTTCTCAGCA GGTTGAACAA CCTACTGGCT CTGTCCTCTG GATGGCCCCA
    GAGGTGATCC GAATGCAGGA TAACAACCCA TTCAGTTTCC AGTCGGATGT CTACTCCTAT
    GGCATCGTAT TGTATGAACT GATGACGGGG GAGCTTCCTT ATTCTCACAT CAACAACCGA
    GATCAGATCA TCTTCATGGT GGGCCGAGGA TATGCCTCCC CAGATCTTAG TAAGCTATAT
    AAGAACTGCC CCAAAGCAAT GAAGAGGCTG GTAGCTGACT GTGTGAAGAA AGTAAAGGAA
    GAGAGGCCTC TTTTTCCCCA GATCCTGTCT TCCATTGAGC TGCTCCAACA CTCTCTACCG
    AAGATCAACC GGAGCGCTTC CGAGCCATCC TTGCATCGGG CAGCCCACAC TGAGGATATC
    AATGCTTGCA CGCTGACCAC GTCCCCGAGG CTGCCTGTCT TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001341624.1
    CDS462..2066
    Translation

    Target ORF information:

    RefSeq Version NM_001354695.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001354695.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT TTCTTGCCGA 
    ACAAGCAAAG AACAGTGTAA AAAAGCACGC TTAGATTGGA ATACTGATGC TGCGTCTTTG
    ATTGGAGAAG AACTTCAAGT AGATTTCCTG GATCATGTTC CCCTCACAAC ACACAACTTT
    GCTCGGAAGA CGTTCCTGAA GCTTGCCTTC TGTGACATCT GTCAGAAATT CCTGCTCAAT
    GGATTTCGAT GTCAGACTTG TGGCTACAAA TTTCATGAGC ACTGTAGCAC CAAAGTACCT
    ACTATGTGTG TGGACTGGAG TAACATCAGA CAACTCTTTT CTCAGCACAG ATATTCTACA
    CCTCACGCCT TCACCTTTAA CACCTCCAGT CCCTCATCTG AAGGTTCCCT CTCCCAGAGG
    CAGAGGTCGA CATCCACACC TAATGTCCAC ATGGTCAGCA CCACCCTGCC TGTGGACAGC
    AGGATGATTG AGGATGCAAT TCGAAGTCAC AGCGAATCAG CCTCACCTTC AGCCCTGTCC
    AGTAGCCCCA ACAATCTGAG CCCAACAGGC TGGTCACAGC CGAAAACCCC CGTGCCAGCA
    CAAAGAGAGC GGGCACCAGT ATCTGGGACC CAGGAGAAAA ACAAAATTAG GCCTCGTGGA
    CAGAGAGATT CAAGCTATTA TTGGGAAATA GAAGCCAGTG AAGTGATGCT GTCCACTCGG
    ATTGGGTCAG GCTCTTTTGG AACTGTTTAT AAGGGTAAAT GGCACGGAGA TGTTGCAGTA
    AAGATCCTAA AGGTTGTCGA CCCAACCCCA GAGCAATTCC AGGCCTTCAG GAATGAGGTG
    GCTGTTCTGC GCAAAACACG GCATGTGAAC ATTCTGCTTT TCATGGGGTA CATGACAAAG
    GACAACCTGG CAATTGTGAC CCAGTGGTGC GAGGGCAGCA GCCTCTACAA ACACCTGCAT
    GTCCAGGAGA CCAAGTTTCA GATGTTCCAG CTAATTGACA TTGCCCGGCA GACGGCTCAG
    GGAATGGACT ATTTGCATGC AAAGAACATC ATCCATAGAG ACATGAAATC CAACAATATA
    TTTCTCCATG AAGGCTTAAC AGTGAAAATT GGAGATTTTG GTTTGGCAAC AGTAAAGTCA
    CGCTGGAGTG GTTCTCAGCA GGTTGAACAA CCTACTGGCT CTGTCCTCTG GATGGCCCCA
    GAGGTGATCC GAATGCAGGA TAACAACCCA TTCAGTTTCC AGTCGGATGT CTACTCCTAT
    GGCATCGTAT TGTATGAACT GATGACGGGG GAGCTTCCTT ATTCTCACAT CAACAACCGA
    GATCAGATCA TCTTCATGGT GGGCCGAGGA TATGCCTCCC CAGATCTTAG TAAGCTATAT
    AAGAACTGCC CCAAAGCAAT GAAGAGGCTG GTAGCTGACT GTGTGAAGAA AGTAAAGGAA
    GAGAGGCCTC TTTTTCCCCA GATCCTGTCT TCCATTGAGC TGCTCCAACA CTCTCTACCG
    AAGATCAACC GGAGCGCTTC CGAGCCATCC TTGCATCGGG CAGCCCACAC TGAGGATATC
    AATGCTTGCA CGCTGACCAC GTCCCCGAGG CTGCCTGTCT TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu37400
    Clone ID Related Accession (Same CDS sequence) XM_005265357.2 , NM_001354693.1 , XM_017006966.1 , NM_001354693.2
    Accession Version XM_005265357.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1848bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(Human)
    Product RAF proto-oncogene serine/threonine-protein kinase isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005265357.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGAGCACA TACAGGGAGC TTGGAAGACG ATCAGCAATG GTTTTGGATT CAAAGATGCC 
    GTGTTTGATG GCTCCAGCTG CATCTCTCCT ACAATAGTTC AGCAGTTTGG CTATCAGCGC
    CGGGCATCAG ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT
    TTCTTGCCGA ACAAGCAAAG AACAGTGGTC AATGTGCGAA ATGGAATGAG CTTGCATGAC
    TGCCTTATGA AAGCACTCAA GGTGAGGGGC CTGCAACCAG AGTGCTGTGC AGTGTTCAGA
    CTTCTCCACG AACACAAAGG TAAAAAAGCA CGCTTAGATT GGAATACTGA TGCTGCGTCT
    TTGATTGGAG AAGAACTTCA AGTAGATTTC CTGGATCATG TTCCCCTCAC AACACACAAC
    TTTGCTCGGA AGACGTTCCT GAAGCTTGCC TTCTGTGACA TCTGTCAGAA ATTCCTGCTC
    AATGGATTTC GATGTCAGAC TTGTGGCTAC AAATTTCATG AGCACTGTAG CACCAAAGTA
    CCTACTATGT GTGTGGACTG GAGTAACATC AGACAACTCT TTTCTCAGCA CAGATATTCT
    ACACCTCACG CCTTCACCTT TAACACCTCC AGTCCCTCAT CTGAAGGTTC CCTCTCCCAG
    AGGCAGAGGT CGACATCCAC ACCTAATGTC CACATGGTCA GCACCACCCT GCCTGTGGAC
    AGCAGGATGA TTGAGGATGC AATTCGAAGT CACAGCGAAT CAGCCTCACC TTCAGCCCTG
    TCCAGTAGCC CCAACAATCT GAGCCCAACA GGCTGGTCAC AGCCGAAAAC CCCCGTGCCA
    GCACAAAGAG AGCGGGCACC AGTATCTGGG ACCCAGGAGA AAAACAAAAT TAGGCCTCGT
    GGACAGAGAG ATTCAAGCTA TTATTGGGAA ATAGAAGCCA GTGAAGTGAT GCTGTCCACT
    CGGATTGGGT CAGGCTCTTT TGGAACTGTT TATAAGGGTA AATGGCACGG AGATGTTGCA
    GTAAAGATCC TAAAGGTTGT CGACCCAACC CCAGAGCAAT TCCAGGCCTT CAGGAATGAG
    GTGGCTGTTC TGCGCAAAAC ACGGCATGTG AACATTCTGC TTTTCATGGG GTACATGACA
    AAGGACAACC TGGCAATTGT GACCCAGTGG TGCGAGGGCA GCAGCCTCTA CAAACACCTG
    CATGTCCAGG AGACCAAGTT TCAGATGTTC CAGCTAATTG ACATTGCCCG GCAGACGGCT
    CAGGGAATGG ACTATTTGCA TGCAAAGAAC ATCATCCATA GAGACATGAA ATCCAACAAT
    ATATTTCTCC ATGAAGGCTT AACAGTGAAA ATTGGAGATT TTGGTTTGGC AACAGTAAAG
    TCACGCTGGA GTGGTTCTCA GCAGGTTGAA CAACCTACTG GCTCTGTCCT CTGGATGGCC
    CCAGAGGTGA TCCGAATGCA GGATAACAAC CCATTCAGTT TCCAGTCGGA TGTCTACTCC
    TATGGCATCG TATTGTATGA ACTGATGACG GGGGAGCTTC CTTATTCTCA CATCAACAAC
    CGAGATCAGA TCATCTTCAT GGTGGGCCGA GGATATGCCT CCCCAGATCT TAGTAAGCTA
    TATAAGAACT GCCCCAAAGC AATGAAGAGG CTGGTAGCTG ACTGTGTGAA GAAAGTAAAG
    GAAGAGAGGC CTCTTTTTCC CCAGATCCTG TCTTCCATTG AGCTGCTCCA ACACTCTCTA
    CCGAAGATCA ACCGGAGCGC TTCCGAGCCA TCCTTGCATC GGGCAGCCCA CACTGAGGAT
    ATCAATGCTT GCACGCTGAC CACGTCCCCG AGGCTGCCTG TCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005265414.1
    CDS332..2179
    Translation

    Target ORF information:

    RefSeq Version XM_005265357.2
    Organism Homo sapiens(Human)
    Definition Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005265357.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGAGCACA TACAGGGAGC TTGGAAGACG ATCAGCAATG GTTTTGGATT CAAAGATGCC 
    GTGTTTGATG GCTCCAGCTG CATCTCTCCT ACAATAGTTC AGCAGTTTGG CTATCAGCGC
    CGGGCATCAG ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT
    TTCTTGCCGA ACAAGCAAAG AACAGTGGTC AATGTGCGAA ATGGAATGAG CTTGCATGAC
    TGCCTTATGA AAGCACTCAA GGTGAGGGGC CTGCAACCAG AGTGCTGTGC AGTGTTCAGA
    CTTCTCCACG AACACAAAGG TAAAAAAGCA CGCTTAGATT GGAATACTGA TGCTGCGTCT
    TTGATTGGAG AAGAACTTCA AGTAGATTTC CTGGATCATG TTCCCCTCAC AACACACAAC
    TTTGCTCGGA AGACGTTCCT GAAGCTTGCC TTCTGTGACA TCTGTCAGAA ATTCCTGCTC
    AATGGATTTC GATGTCAGAC TTGTGGCTAC AAATTTCATG AGCACTGTAG CACCAAAGTA
    CCTACTATGT GTGTGGACTG GAGTAACATC AGACAACTCT TTTCTCAGCA CAGATATTCT
    ACACCTCACG CCTTCACCTT TAACACCTCC AGTCCCTCAT CTGAAGGTTC CCTCTCCCAG
    AGGCAGAGGT CGACATCCAC ACCTAATGTC CACATGGTCA GCACCACCCT GCCTGTGGAC
    AGCAGGATGA TTGAGGATGC AATTCGAAGT CACAGCGAAT CAGCCTCACC TTCAGCCCTG
    TCCAGTAGCC CCAACAATCT GAGCCCAACA GGCTGGTCAC AGCCGAAAAC CCCCGTGCCA
    GCACAAAGAG AGCGGGCACC AGTATCTGGG ACCCAGGAGA AAAACAAAAT TAGGCCTCGT
    GGACAGAGAG ATTCAAGCTA TTATTGGGAA ATAGAAGCCA GTGAAGTGAT GCTGTCCACT
    CGGATTGGGT CAGGCTCTTT TGGAACTGTT TATAAGGGTA AATGGCACGG AGATGTTGCA
    GTAAAGATCC TAAAGGTTGT CGACCCAACC CCAGAGCAAT TCCAGGCCTT CAGGAATGAG
    GTGGCTGTTC TGCGCAAAAC ACGGCATGTG AACATTCTGC TTTTCATGGG GTACATGACA
    AAGGACAACC TGGCAATTGT GACCCAGTGG TGCGAGGGCA GCAGCCTCTA CAAACACCTG
    CATGTCCAGG AGACCAAGTT TCAGATGTTC CAGCTAATTG ACATTGCCCG GCAGACGGCT
    CAGGGAATGG ACTATTTGCA TGCAAAGAAC ATCATCCATA GAGACATGAA ATCCAACAAT
    ATATTTCTCC ATGAAGGCTT AACAGTGAAA ATTGGAGATT TTGGTTTGGC AACAGTAAAG
    TCACGCTGGA GTGGTTCTCA GCAGGTTGAA CAACCTACTG GCTCTGTCCT CTGGATGGCC
    CCAGAGGTGA TCCGAATGCA GGATAACAAC CCATTCAGTT TCCAGTCGGA TGTCTACTCC
    TATGGCATCG TATTGTATGA ACTGATGACG GGGGAGCTTC CTTATTCTCA CATCAACAAC
    CGAGATCAGA TCATCTTCAT GGTGGGCCGA GGATATGCCT CCCCAGATCT TAGTAAGCTA
    TATAAGAACT GCCCCAAAGC AATGAAGAGG CTGGTAGCTG ACTGTGTGAA GAAAGTAAAG
    GAAGAGAGGC CTCTTTTTCC CCAGATCCTG TCTTCCATTG AGCTGCTCCA ACACTCTCTA
    CCGAAGATCA ACCGGAGCGC TTCCGAGCCA TCCTTGCATC GGGCAGCCCA CACTGAGGAT
    ATCAATGCTT GCACGCTGAC CACGTCCCCG AGGCTGCCTG TCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu37400
    Clone ID Related Accession (Same CDS sequence) XM_005265357.2 , NM_001354693.1 , XM_017006966.1 , NM_001354693.2
    Accession Version XM_017006966.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1848bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product RAF proto-oncogene serine/threonine-protein kinase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGAGCACA TACAGGGAGC TTGGAAGACG ATCAGCAATG GTTTTGGATT CAAAGATGCC 
    GTGTTTGATG GCTCCAGCTG CATCTCTCCT ACAATAGTTC AGCAGTTTGG CTATCAGCGC
    CGGGCATCAG ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT
    TTCTTGCCGA ACAAGCAAAG AACAGTGGTC AATGTGCGAA ATGGAATGAG CTTGCATGAC
    TGCCTTATGA AAGCACTCAA GGTGAGGGGC CTGCAACCAG AGTGCTGTGC AGTGTTCAGA
    CTTCTCCACG AACACAAAGG TAAAAAAGCA CGCTTAGATT GGAATACTGA TGCTGCGTCT
    TTGATTGGAG AAGAACTTCA AGTAGATTTC CTGGATCATG TTCCCCTCAC AACACACAAC
    TTTGCTCGGA AGACGTTCCT GAAGCTTGCC TTCTGTGACA TCTGTCAGAA ATTCCTGCTC
    AATGGATTTC GATGTCAGAC TTGTGGCTAC AAATTTCATG AGCACTGTAG CACCAAAGTA
    CCTACTATGT GTGTGGACTG GAGTAACATC AGACAACTCT TTTCTCAGCA CAGATATTCT
    ACACCTCACG CCTTCACCTT TAACACCTCC AGTCCCTCAT CTGAAGGTTC CCTCTCCCAG
    AGGCAGAGGT CGACATCCAC ACCTAATGTC CACATGGTCA GCACCACCCT GCCTGTGGAC
    AGCAGGATGA TTGAGGATGC AATTCGAAGT CACAGCGAAT CAGCCTCACC TTCAGCCCTG
    TCCAGTAGCC CCAACAATCT GAGCCCAACA GGCTGGTCAC AGCCGAAAAC CCCCGTGCCA
    GCACAAAGAG AGCGGGCACC AGTATCTGGG ACCCAGGAGA AAAACAAAAT TAGGCCTCGT
    GGACAGAGAG ATTCAAGCTA TTATTGGGAA ATAGAAGCCA GTGAAGTGAT GCTGTCCACT
    CGGATTGGGT CAGGCTCTTT TGGAACTGTT TATAAGGGTA AATGGCACGG AGATGTTGCA
    GTAAAGATCC TAAAGGTTGT CGACCCAACC CCAGAGCAAT TCCAGGCCTT CAGGAATGAG
    GTGGCTGTTC TGCGCAAAAC ACGGCATGTG AACATTCTGC TTTTCATGGG GTACATGACA
    AAGGACAACC TGGCAATTGT GACCCAGTGG TGCGAGGGCA GCAGCCTCTA CAAACACCTG
    CATGTCCAGG AGACCAAGTT TCAGATGTTC CAGCTAATTG ACATTGCCCG GCAGACGGCT
    CAGGGAATGG ACTATTTGCA TGCAAAGAAC ATCATCCATA GAGACATGAA ATCCAACAAT
    ATATTTCTCC ATGAAGGCTT AACAGTGAAA ATTGGAGATT TTGGTTTGGC AACAGTAAAG
    TCACGCTGGA GTGGTTCTCA GCAGGTTGAA CAACCTACTG GCTCTGTCCT CTGGATGGCC
    CCAGAGGTGA TCCGAATGCA GGATAACAAC CCATTCAGTT TCCAGTCGGA TGTCTACTCC
    TATGGCATCG TATTGTATGA ACTGATGACG GGGGAGCTTC CTTATTCTCA CATCAACAAC
    CGAGATCAGA TCATCTTCAT GGTGGGCCGA GGATATGCCT CCCCAGATCT TAGTAAGCTA
    TATAAGAACT GCCCCAAAGC AATGAAGAGG CTGGTAGCTG ACTGTGTGAA GAAAGTAAAG
    GAAGAGAGGC CTCTTTTTCC CCAGATCCTG TCTTCCATTG AGCTGCTCCA ACACTCTCTA
    CCGAAGATCA ACCGGAGCGC TTCCGAGCCA TCCTTGCATC GGGCAGCCCA CACTGAGGAT
    ATCAATGCTT GCACGCTGAC CACGTCCCCG AGGCTGCCTG TCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016862455.1
    CDS120..1967
    Translation

    Target ORF information:

    RefSeq Version XM_017006966.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017006966.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGAGCACA TACAGGGAGC TTGGAAGACG ATCAGCAATG GTTTTGGATT CAAAGATGCC 
    GTGTTTGATG GCTCCAGCTG CATCTCTCCT ACAATAGTTC AGCAGTTTGG CTATCAGCGC
    CGGGCATCAG ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT
    TTCTTGCCGA ACAAGCAAAG AACAGTGGTC AATGTGCGAA ATGGAATGAG CTTGCATGAC
    TGCCTTATGA AAGCACTCAA GGTGAGGGGC CTGCAACCAG AGTGCTGTGC AGTGTTCAGA
    CTTCTCCACG AACACAAAGG TAAAAAAGCA CGCTTAGATT GGAATACTGA TGCTGCGTCT
    TTGATTGGAG AAGAACTTCA AGTAGATTTC CTGGATCATG TTCCCCTCAC AACACACAAC
    TTTGCTCGGA AGACGTTCCT GAAGCTTGCC TTCTGTGACA TCTGTCAGAA ATTCCTGCTC
    AATGGATTTC GATGTCAGAC TTGTGGCTAC AAATTTCATG AGCACTGTAG CACCAAAGTA
    CCTACTATGT GTGTGGACTG GAGTAACATC AGACAACTCT TTTCTCAGCA CAGATATTCT
    ACACCTCACG CCTTCACCTT TAACACCTCC AGTCCCTCAT CTGAAGGTTC CCTCTCCCAG
    AGGCAGAGGT CGACATCCAC ACCTAATGTC CACATGGTCA GCACCACCCT GCCTGTGGAC
    AGCAGGATGA TTGAGGATGC AATTCGAAGT CACAGCGAAT CAGCCTCACC TTCAGCCCTG
    TCCAGTAGCC CCAACAATCT GAGCCCAACA GGCTGGTCAC AGCCGAAAAC CCCCGTGCCA
    GCACAAAGAG AGCGGGCACC AGTATCTGGG ACCCAGGAGA AAAACAAAAT TAGGCCTCGT
    GGACAGAGAG ATTCAAGCTA TTATTGGGAA ATAGAAGCCA GTGAAGTGAT GCTGTCCACT
    CGGATTGGGT CAGGCTCTTT TGGAACTGTT TATAAGGGTA AATGGCACGG AGATGTTGCA
    GTAAAGATCC TAAAGGTTGT CGACCCAACC CCAGAGCAAT TCCAGGCCTT CAGGAATGAG
    GTGGCTGTTC TGCGCAAAAC ACGGCATGTG AACATTCTGC TTTTCATGGG GTACATGACA
    AAGGACAACC TGGCAATTGT GACCCAGTGG TGCGAGGGCA GCAGCCTCTA CAAACACCTG
    CATGTCCAGG AGACCAAGTT TCAGATGTTC CAGCTAATTG ACATTGCCCG GCAGACGGCT
    CAGGGAATGG ACTATTTGCA TGCAAAGAAC ATCATCCATA GAGACATGAA ATCCAACAAT
    ATATTTCTCC ATGAAGGCTT AACAGTGAAA ATTGGAGATT TTGGTTTGGC AACAGTAAAG
    TCACGCTGGA GTGGTTCTCA GCAGGTTGAA CAACCTACTG GCTCTGTCCT CTGGATGGCC
    CCAGAGGTGA TCCGAATGCA GGATAACAAC CCATTCAGTT TCCAGTCGGA TGTCTACTCC
    TATGGCATCG TATTGTATGA ACTGATGACG GGGGAGCTTC CTTATTCTCA CATCAACAAC
    CGAGATCAGA TCATCTTCAT GGTGGGCCGA GGATATGCCT CCCCAGATCT TAGTAAGCTA
    TATAAGAACT GCCCCAAAGC AATGAAGAGG CTGGTAGCTG ACTGTGTGAA GAAAGTAAAG
    GAAGAGAGGC CTCTTTTTCC CCAGATCCTG TCTTCCATTG AGCTGCTCCA ACACTCTCTA
    CCGAAGATCA ACCGGAGCGC TTCCGAGCCA TCCTTGCATC GGGCAGCCCA CACTGAGGAT
    ATCAATGCTT GCACGCTGAC CACGTCCCCG AGGCTGCCTG TCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu37401
    Clone ID Related Accession (Same CDS sequence) XM_011533975.2 , NM_001354691.2 , NM_001354691.1 , NM_001354692.2 , NM_001354692.1 , XM_005265358.4
    Accession Version XM_011533975.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1704bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(Human)
    Product RAF proto-oncogene serine/threonine-protein kinase isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011533975.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT TTCTTGCCGA 
    ACAAGCAAAG AACAGTGTAA AAAAGCACGC TTAGATTGGA ATACTGATGC TGCGTCTTTG
    ATTGGAGAAG AACTTCAAGT AGATTTCCTG GATCATGTTC CCCTCACAAC ACACAACTTT
    GCTCGGAAGA CGTTCCTGAA GCTTGCCTTC TGTGACATCT GTCAGAAATT CCTGCTCAAT
    GGATTTCGAT GTCAGACTTG TGGCTACAAA TTTCATGAGC ACTGTAGCAC CAAAGTACCT
    ACTATGTGTG TGGACTGGAG TAACATCAGA CAACTCTTAT TGTTTCCAAA TTCCACTATT
    GGTGATAGTG GAGTCCCAGC ACTACCTTCT TTGACTATGC GTCGTATGCG AGAGTCTGTT
    TCCAGGATGC CTGTTAGTTC TCAGCACAGA TATTCTACAC CTCACGCCTT CACCTTTAAC
    ACCTCCAGTC CCTCATCTGA AGGTTCCCTC TCCCAGAGGC AGAGGTCGAC ATCCACACCT
    AATGTCCACA TGGTCAGCAC CACCCTGCCT GTGGACAGCA GGATGATTGA GGATGCAATT
    CGAAGTCACA GCGAATCAGC CTCACCTTCA GCCCTGTCCA GTAGCCCCAA CAATCTGAGC
    CCAACAGGCT GGTCACAGCC GAAAACCCCC GTGCCAGCAC AAAGAGAGCG GGCACCAGTA
    TCTGGGACCC AGGAGAAAAA CAAAATTAGG CCTCGTGGAC AGAGAGATTC AAGCTATTAT
    TGGGAAATAG AAGCCAGTGA AGTGATGCTG TCCACTCGGA TTGGGTCAGG CTCTTTTGGA
    ACTGTTTATA AGGGTAAATG GCACGGAGAT GTTGCAGTAA AGATCCTAAA GGTTGTCGAC
    CCAACCCCAG AGCAATTCCA GGCCTTCAGG AATGAGGTGG CTGTTCTGCG CAAAACACGG
    CATGTGAACA TTCTGCTTTT CATGGGGTAC ATGACAAAGG ACAACCTGGC AATTGTGACC
    CAGTGGTGCG AGGGCAGCAG CCTCTACAAA CACCTGCATG TCCAGGAGAC CAAGTTTCAG
    ATGTTCCAGC TAATTGACAT TGCCCGGCAG ACGGCTCAGG GAATGGACTA TTTGCATGCA
    AAGAACATCA TCCATAGAGA CATGAAATCC AACAATATAT TTCTCCATGA AGGCTTAACA
    GTGAAAATTG GAGATTTTGG TTTGGCAACA GTAAAGTCAC GCTGGAGTGG TTCTCAGCAG
    GTTGAACAAC CTACTGGCTC TGTCCTCTGG ATGGCCCCAG AGGTGATCCG AATGCAGGAT
    AACAACCCAT TCAGTTTCCA GTCGGATGTC TACTCCTATG GCATCGTATT GTATGAACTG
    ATGACGGGGG AGCTTCCTTA TTCTCACATC AACAACCGAG ATCAGATCAT CTTCATGGTG
    GGCCGAGGAT ATGCCTCCCC AGATCTTAGT AAGCTATATA AGAACTGCCC CAAAGCAATG
    AAGAGGCTGG TAGCTGACTG TGTGAAGAAA GTAAAGGAAG AGAGGCCTCT TTTTCCCCAG
    ATCCTGTCTT CCATTGAGCT GCTCCAACAC TCTCTACCGA AGATCAACCG GAGCGCTTCC
    GAGCCATCCT TGCATCGGGC AGCCCACACT GAGGATATCA ATGCTTGCAC GCTGACCACG
    TCCCCGAGGC TGCCTGTCTT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011532277.1
    CDS591..2294
    Translation

    Target ORF information:

    RefSeq Version XM_011533975.2
    Organism Homo sapiens(Human)
    Definition Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011533975.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT TTCTTGCCGA 
    ACAAGCAAAG AACAGTGTAA AAAAGCACGC TTAGATTGGA ATACTGATGC TGCGTCTTTG
    ATTGGAGAAG AACTTCAAGT AGATTTCCTG GATCATGTTC CCCTCACAAC ACACAACTTT
    GCTCGGAAGA CGTTCCTGAA GCTTGCCTTC TGTGACATCT GTCAGAAATT CCTGCTCAAT
    GGATTTCGAT GTCAGACTTG TGGCTACAAA TTTCATGAGC ACTGTAGCAC CAAAGTACCT
    ACTATGTGTG TGGACTGGAG TAACATCAGA CAACTCTTAT TGTTTCCAAA TTCCACTATT
    GGTGATAGTG GAGTCCCAGC ACTACCTTCT TTGACTATGC GTCGTATGCG AGAGTCTGTT
    TCCAGGATGC CTGTTAGTTC TCAGCACAGA TATTCTACAC CTCACGCCTT CACCTTTAAC
    ACCTCCAGTC CCTCATCTGA AGGTTCCCTC TCCCAGAGGC AGAGGTCGAC ATCCACACCT
    AATGTCCACA TGGTCAGCAC CACCCTGCCT GTGGACAGCA GGATGATTGA GGATGCAATT
    CGAAGTCACA GCGAATCAGC CTCACCTTCA GCCCTGTCCA GTAGCCCCAA CAATCTGAGC
    CCAACAGGCT GGTCACAGCC GAAAACCCCC GTGCCAGCAC AAAGAGAGCG GGCACCAGTA
    TCTGGGACCC AGGAGAAAAA CAAAATTAGG CCTCGTGGAC AGAGAGATTC AAGCTATTAT
    TGGGAAATAG AAGCCAGTGA AGTGATGCTG TCCACTCGGA TTGGGTCAGG CTCTTTTGGA
    ACTGTTTATA AGGGTAAATG GCACGGAGAT GTTGCAGTAA AGATCCTAAA GGTTGTCGAC
    CCAACCCCAG AGCAATTCCA GGCCTTCAGG AATGAGGTGG CTGTTCTGCG CAAAACACGG
    CATGTGAACA TTCTGCTTTT CATGGGGTAC ATGACAAAGG ACAACCTGGC AATTGTGACC
    CAGTGGTGCG AGGGCAGCAG CCTCTACAAA CACCTGCATG TCCAGGAGAC CAAGTTTCAG
    ATGTTCCAGC TAATTGACAT TGCCCGGCAG ACGGCTCAGG GAATGGACTA TTTGCATGCA
    AAGAACATCA TCCATAGAGA CATGAAATCC AACAATATAT TTCTCCATGA AGGCTTAACA
    GTGAAAATTG GAGATTTTGG TTTGGCAACA GTAAAGTCAC GCTGGAGTGG TTCTCAGCAG
    GTTGAACAAC CTACTGGCTC TGTCCTCTGG ATGGCCCCAG AGGTGATCCG AATGCAGGAT
    AACAACCCAT TCAGTTTCCA GTCGGATGTC TACTCCTATG GCATCGTATT GTATGAACTG
    ATGACGGGGG AGCTTCCTTA TTCTCACATC AACAACCGAG ATCAGATCAT CTTCATGGTG
    GGCCGAGGAT ATGCCTCCCC AGATCTTAGT AAGCTATATA AGAACTGCCC CAAAGCAATG
    AAGAGGCTGG TAGCTGACTG TGTGAAGAAA GTAAAGGAAG AGAGGCCTCT TTTTCCCCAG
    ATCCTGTCTT CCATTGAGCT GCTCCAACAC TCTCTACCGA AGATCAACCG GAGCGCTTCC
    GAGCCATCCT TGCATCGGGC AGCCCACACT GAGGATATCA ATGCTTGCAC GCTGACCACG
    TCCCCGAGGC TGCCTGTCTT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu37401
    Clone ID Related Accession (Same CDS sequence) XM_011533975.2 , NM_001354691.2 , NM_001354691.1 , NM_001354692.2 , NM_001354692.1 , XM_005265358.4
    Accession Version XM_005265358.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1704bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(Human)
    Product RAF proto-oncogene serine/threonine-protein kinase isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005265358.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT TTCTTGCCGA 
    ACAAGCAAAG AACAGTGTAA AAAAGCACGC TTAGATTGGA ATACTGATGC TGCGTCTTTG
    ATTGGAGAAG AACTTCAAGT AGATTTCCTG GATCATGTTC CCCTCACAAC ACACAACTTT
    GCTCGGAAGA CGTTCCTGAA GCTTGCCTTC TGTGACATCT GTCAGAAATT CCTGCTCAAT
    GGATTTCGAT GTCAGACTTG TGGCTACAAA TTTCATGAGC ACTGTAGCAC CAAAGTACCT
    ACTATGTGTG TGGACTGGAG TAACATCAGA CAACTCTTAT TGTTTCCAAA TTCCACTATT
    GGTGATAGTG GAGTCCCAGC ACTACCTTCT TTGACTATGC GTCGTATGCG AGAGTCTGTT
    TCCAGGATGC CTGTTAGTTC TCAGCACAGA TATTCTACAC CTCACGCCTT CACCTTTAAC
    ACCTCCAGTC CCTCATCTGA AGGTTCCCTC TCCCAGAGGC AGAGGTCGAC ATCCACACCT
    AATGTCCACA TGGTCAGCAC CACCCTGCCT GTGGACAGCA GGATGATTGA GGATGCAATT
    CGAAGTCACA GCGAATCAGC CTCACCTTCA GCCCTGTCCA GTAGCCCCAA CAATCTGAGC
    CCAACAGGCT GGTCACAGCC GAAAACCCCC GTGCCAGCAC AAAGAGAGCG GGCACCAGTA
    TCTGGGACCC AGGAGAAAAA CAAAATTAGG CCTCGTGGAC AGAGAGATTC AAGCTATTAT
    TGGGAAATAG AAGCCAGTGA AGTGATGCTG TCCACTCGGA TTGGGTCAGG CTCTTTTGGA
    ACTGTTTATA AGGGTAAATG GCACGGAGAT GTTGCAGTAA AGATCCTAAA GGTTGTCGAC
    CCAACCCCAG AGCAATTCCA GGCCTTCAGG AATGAGGTGG CTGTTCTGCG CAAAACACGG
    CATGTGAACA TTCTGCTTTT CATGGGGTAC ATGACAAAGG ACAACCTGGC AATTGTGACC
    CAGTGGTGCG AGGGCAGCAG CCTCTACAAA CACCTGCATG TCCAGGAGAC CAAGTTTCAG
    ATGTTCCAGC TAATTGACAT TGCCCGGCAG ACGGCTCAGG GAATGGACTA TTTGCATGCA
    AAGAACATCA TCCATAGAGA CATGAAATCC AACAATATAT TTCTCCATGA AGGCTTAACA
    GTGAAAATTG GAGATTTTGG TTTGGCAACA GTAAAGTCAC GCTGGAGTGG TTCTCAGCAG
    GTTGAACAAC CTACTGGCTC TGTCCTCTGG ATGGCCCCAG AGGTGATCCG AATGCAGGAT
    AACAACCCAT TCAGTTTCCA GTCGGATGTC TACTCCTATG GCATCGTATT GTATGAACTG
    ATGACGGGGG AGCTTCCTTA TTCTCACATC AACAACCGAG ATCAGATCAT CTTCATGGTG
    GGCCGAGGAT ATGCCTCCCC AGATCTTAGT AAGCTATATA AGAACTGCCC CAAAGCAATG
    AAGAGGCTGG TAGCTGACTG TGTGAAGAAA GTAAAGGAAG AGAGGCCTCT TTTTCCCCAG
    ATCCTGTCTT CCATTGAGCT GCTCCAACAC TCTCTACCGA AGATCAACCG GAGCGCTTCC
    GAGCCATCCT TGCATCGGGC AGCCCACACT GAGGATATCA ATGCTTGCAC GCTGACCACG
    TCCCCGAGGC TGCCTGTCTT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005265415.1
    CDS541..2244
    Translation

    Target ORF information:

    RefSeq Version XM_005265358.4
    Organism Homo sapiens(Human)
    Definition Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005265358.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT TTCTTGCCGA 
    ACAAGCAAAG AACAGTGTAA AAAAGCACGC TTAGATTGGA ATACTGATGC TGCGTCTTTG
    ATTGGAGAAG AACTTCAAGT AGATTTCCTG GATCATGTTC CCCTCACAAC ACACAACTTT
    GCTCGGAAGA CGTTCCTGAA GCTTGCCTTC TGTGACATCT GTCAGAAATT CCTGCTCAAT
    GGATTTCGAT GTCAGACTTG TGGCTACAAA TTTCATGAGC ACTGTAGCAC CAAAGTACCT
    ACTATGTGTG TGGACTGGAG TAACATCAGA CAACTCTTAT TGTTTCCAAA TTCCACTATT
    GGTGATAGTG GAGTCCCAGC ACTACCTTCT TTGACTATGC GTCGTATGCG AGAGTCTGTT
    TCCAGGATGC CTGTTAGTTC TCAGCACAGA TATTCTACAC CTCACGCCTT CACCTTTAAC
    ACCTCCAGTC CCTCATCTGA AGGTTCCCTC TCCCAGAGGC AGAGGTCGAC ATCCACACCT
    AATGTCCACA TGGTCAGCAC CACCCTGCCT GTGGACAGCA GGATGATTGA GGATGCAATT
    CGAAGTCACA GCGAATCAGC CTCACCTTCA GCCCTGTCCA GTAGCCCCAA CAATCTGAGC
    CCAACAGGCT GGTCACAGCC GAAAACCCCC GTGCCAGCAC AAAGAGAGCG GGCACCAGTA
    TCTGGGACCC AGGAGAAAAA CAAAATTAGG CCTCGTGGAC AGAGAGATTC AAGCTATTAT
    TGGGAAATAG AAGCCAGTGA AGTGATGCTG TCCACTCGGA TTGGGTCAGG CTCTTTTGGA
    ACTGTTTATA AGGGTAAATG GCACGGAGAT GTTGCAGTAA AGATCCTAAA GGTTGTCGAC
    CCAACCCCAG AGCAATTCCA GGCCTTCAGG AATGAGGTGG CTGTTCTGCG CAAAACACGG
    CATGTGAACA TTCTGCTTTT CATGGGGTAC ATGACAAAGG ACAACCTGGC AATTGTGACC
    CAGTGGTGCG AGGGCAGCAG CCTCTACAAA CACCTGCATG TCCAGGAGAC CAAGTTTCAG
    ATGTTCCAGC TAATTGACAT TGCCCGGCAG ACGGCTCAGG GAATGGACTA TTTGCATGCA
    AAGAACATCA TCCATAGAGA CATGAAATCC AACAATATAT TTCTCCATGA AGGCTTAACA
    GTGAAAATTG GAGATTTTGG TTTGGCAACA GTAAAGTCAC GCTGGAGTGG TTCTCAGCAG
    GTTGAACAAC CTACTGGCTC TGTCCTCTGG ATGGCCCCAG AGGTGATCCG AATGCAGGAT
    AACAACCCAT TCAGTTTCCA GTCGGATGTC TACTCCTATG GCATCGTATT GTATGAACTG
    ATGACGGGGG AGCTTCCTTA TTCTCACATC AACAACCGAG ATCAGATCAT CTTCATGGTG
    GGCCGAGGAT ATGCCTCCCC AGATCTTAGT AAGCTATATA AGAACTGCCC CAAAGCAATG
    AAGAGGCTGG TAGCTGACTG TGTGAAGAAA GTAAAGGAAG AGAGGCCTCT TTTTCCCCAG
    ATCCTGTCTT CCATTGAGCT GCTCCAACAC TCTCTACCGA AGATCAACCG GAGCGCTTCC
    GAGCCATCCT TGCATCGGGC AGCCCACACT GAGGATATCA ATGCTTGCAC GCTGACCACG
    TCCCCGAGGC TGCCTGTCTT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu37402
    Clone ID Related Accession (Same CDS sequence) NM_001354695.1 , XM_005265359.4 , NM_001354695.2
    Accession Version XM_005265359.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1605bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(Human)
    Product RAF proto-oncogene serine/threonine-protein kinase isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005265359.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT TTCTTGCCGA 
    ACAAGCAAAG AACAGTGTAA AAAAGCACGC TTAGATTGGA ATACTGATGC TGCGTCTTTG
    ATTGGAGAAG AACTTCAAGT AGATTTCCTG GATCATGTTC CCCTCACAAC ACACAACTTT
    GCTCGGAAGA CGTTCCTGAA GCTTGCCTTC TGTGACATCT GTCAGAAATT CCTGCTCAAT
    GGATTTCGAT GTCAGACTTG TGGCTACAAA TTTCATGAGC ACTGTAGCAC CAAAGTACCT
    ACTATGTGTG TGGACTGGAG TAACATCAGA CAACTCTTTT CTCAGCACAG ATATTCTACA
    CCTCACGCCT TCACCTTTAA CACCTCCAGT CCCTCATCTG AAGGTTCCCT CTCCCAGAGG
    CAGAGGTCGA CATCCACACC TAATGTCCAC ATGGTCAGCA CCACCCTGCC TGTGGACAGC
    AGGATGATTG AGGATGCAAT TCGAAGTCAC AGCGAATCAG CCTCACCTTC AGCCCTGTCC
    AGTAGCCCCA ACAATCTGAG CCCAACAGGC TGGTCACAGC CGAAAACCCC CGTGCCAGCA
    CAAAGAGAGC GGGCACCAGT ATCTGGGACC CAGGAGAAAA ACAAAATTAG GCCTCGTGGA
    CAGAGAGATT CAAGCTATTA TTGGGAAATA GAAGCCAGTG AAGTGATGCT GTCCACTCGG
    ATTGGGTCAG GCTCTTTTGG AACTGTTTAT AAGGGTAAAT GGCACGGAGA TGTTGCAGTA
    AAGATCCTAA AGGTTGTCGA CCCAACCCCA GAGCAATTCC AGGCCTTCAG GAATGAGGTG
    GCTGTTCTGC GCAAAACACG GCATGTGAAC ATTCTGCTTT TCATGGGGTA CATGACAAAG
    GACAACCTGG CAATTGTGAC CCAGTGGTGC GAGGGCAGCA GCCTCTACAA ACACCTGCAT
    GTCCAGGAGA CCAAGTTTCA GATGTTCCAG CTAATTGACA TTGCCCGGCA GACGGCTCAG
    GGAATGGACT ATTTGCATGC AAAGAACATC ATCCATAGAG ACATGAAATC CAACAATATA
    TTTCTCCATG AAGGCTTAAC AGTGAAAATT GGAGATTTTG GTTTGGCAAC AGTAAAGTCA
    CGCTGGAGTG GTTCTCAGCA GGTTGAACAA CCTACTGGCT CTGTCCTCTG GATGGCCCCA
    GAGGTGATCC GAATGCAGGA TAACAACCCA TTCAGTTTCC AGTCGGATGT CTACTCCTAT
    GGCATCGTAT TGTATGAACT GATGACGGGG GAGCTTCCTT ATTCTCACAT CAACAACCGA
    GATCAGATCA TCTTCATGGT GGGCCGAGGA TATGCCTCCC CAGATCTTAG TAAGCTATAT
    AAGAACTGCC CCAAAGCAAT GAAGAGGCTG GTAGCTGACT GTGTGAAGAA AGTAAAGGAA
    GAGAGGCCTC TTTTTCCCCA GATCCTGTCT TCCATTGAGC TGCTCCAACA CTCTCTACCG
    AAGATCAACC GGAGCGCTTC CGAGCCATCC TTGCATCGGG CAGCCCACAC TGAGGATATC
    AATGCTTGCA CGCTGACCAC GTCCCCGAGG CTGCCTGTCT TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005265416.1
    CDS541..2145
    Translation

    Target ORF information:

    RefSeq Version XM_005265359.4
    Organism Homo sapiens(Human)
    Definition Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005265359.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT TTCTTGCCGA 
    ACAAGCAAAG AACAGTGTAA AAAAGCACGC TTAGATTGGA ATACTGATGC TGCGTCTTTG
    ATTGGAGAAG AACTTCAAGT AGATTTCCTG GATCATGTTC CCCTCACAAC ACACAACTTT
    GCTCGGAAGA CGTTCCTGAA GCTTGCCTTC TGTGACATCT GTCAGAAATT CCTGCTCAAT
    GGATTTCGAT GTCAGACTTG TGGCTACAAA TTTCATGAGC ACTGTAGCAC CAAAGTACCT
    ACTATGTGTG TGGACTGGAG TAACATCAGA CAACTCTTTT CTCAGCACAG ATATTCTACA
    CCTCACGCCT TCACCTTTAA CACCTCCAGT CCCTCATCTG AAGGTTCCCT CTCCCAGAGG
    CAGAGGTCGA CATCCACACC TAATGTCCAC ATGGTCAGCA CCACCCTGCC TGTGGACAGC
    AGGATGATTG AGGATGCAAT TCGAAGTCAC AGCGAATCAG CCTCACCTTC AGCCCTGTCC
    AGTAGCCCCA ACAATCTGAG CCCAACAGGC TGGTCACAGC CGAAAACCCC CGTGCCAGCA
    CAAAGAGAGC GGGCACCAGT ATCTGGGACC CAGGAGAAAA ACAAAATTAG GCCTCGTGGA
    CAGAGAGATT CAAGCTATTA TTGGGAAATA GAAGCCAGTG AAGTGATGCT GTCCACTCGG
    ATTGGGTCAG GCTCTTTTGG AACTGTTTAT AAGGGTAAAT GGCACGGAGA TGTTGCAGTA
    AAGATCCTAA AGGTTGTCGA CCCAACCCCA GAGCAATTCC AGGCCTTCAG GAATGAGGTG
    GCTGTTCTGC GCAAAACACG GCATGTGAAC ATTCTGCTTT TCATGGGGTA CATGACAAAG
    GACAACCTGG CAATTGTGAC CCAGTGGTGC GAGGGCAGCA GCCTCTACAA ACACCTGCAT
    GTCCAGGAGA CCAAGTTTCA GATGTTCCAG CTAATTGACA TTGCCCGGCA GACGGCTCAG
    GGAATGGACT ATTTGCATGC AAAGAACATC ATCCATAGAG ACATGAAATC CAACAATATA
    TTTCTCCATG AAGGCTTAAC AGTGAAAATT GGAGATTTTG GTTTGGCAAC AGTAAAGTCA
    CGCTGGAGTG GTTCTCAGCA GGTTGAACAA CCTACTGGCT CTGTCCTCTG GATGGCCCCA
    GAGGTGATCC GAATGCAGGA TAACAACCCA TTCAGTTTCC AGTCGGATGT CTACTCCTAT
    GGCATCGTAT TGTATGAACT GATGACGGGG GAGCTTCCTT ATTCTCACAT CAACAACCGA
    GATCAGATCA TCTTCATGGT GGGCCGAGGA TATGCCTCCC CAGATCTTAG TAAGCTATAT
    AAGAACTGCC CCAAAGCAAT GAAGAGGCTG GTAGCTGACT GTGTGAAGAA AGTAAAGGAA
    GAGAGGCCTC TTTTTCCCCA GATCCTGTCT TCCATTGAGC TGCTCCAACA CTCTCTACCG
    AAGATCAACC GGAGCGCTTC CGAGCCATCC TTGCATCGGG CAGCCCACAC TGAGGATATC
    AATGCTTGCA CGCTGACCAC GTCCCCGAGG CTGCCTGTCT TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu103019
    Clone ID Related Accession (Same CDS sequence) NM_001354689.1 , NM_001354689.2 , NM_001354689.3
    Accession Version NM_001354689.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2007bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product RAF proto-oncogene serine/threonine-protein kinase isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC026170.5 and AC018500.4. Transcript Variant: This variant (1) encodes the longest isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.160683.1, SRR1660807.161082.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAGCACA TACAGGGAGC TTGGAAGACG ATCAGCAATG GTTTTGGATT CAAAGATGCC 
    GTGTTTGATG GCTCCAGCTG CATCTCTCCT ACAATAGTTC AGCAGTTTGG CTATCAGCGC
    CGGGCATCAG ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT
    TTCTTGCCGA ACAAGCAAAG AACAGTGGTC AATGTGCGAA ATGGAATGAG CTTGCATGAC
    TGCCTTATGA AAGCACTCAA GGTGAGGGGC CTGCAACCAG AGTGCTGTGC AGTGTTCAGA
    CTTCTCCACG AACACAAAGG TAAAAAAGCA CGCTTAGATT GGAATACTGA TGCTGCGTCT
    TTGATTGGAG AAGAACTTCA AGTAGATTTC CTGGATCATG TTCCCCTCAC AACACACAAC
    TTTGCTCGGA AGACGTTCCT GAAGCTTGCC TTCTGTGACA TCTGTCAGAA ATTCCTGCTC
    AATGGATTTC GATGTCAGAC TTGTGGCTAC AAATTTCATG AGCACTGTAG CACCAAAGTA
    CCTACTATGT GTGTGGACTG GAGTAACATC AGACAACTCT TATTGTTTCC AAATTCCACT
    ATTGGTGATA GTGGAGTCCC AGCACTACCT TCTTTGACTA TGCGTCGTAT GCGAGAGTCT
    GTTTCCAGGA TGCCTGTTAG TTCTCAGCAC AGATATTCTA CACCTCACGC CTTCACCTTT
    AACACCTCCA GTCCCTCATC TGAAGGTTCC CTCTCCCAGA GGCAGAGGTC GACATCCACA
    CCTAATGTCC ACATGGTCAG CACCACCCTG CCTGTGGACA GCAGGATGAT TGAGAATAAC
    AACCTGAGTG CTTCTCCCAG GGCGTGGTCC AGACGATTTT GTTTGAGGGG AAGAGATGCA
    ATTCGAAGTC ACAGCGAATC AGCCTCACCT TCAGCCCTGT CCAGTAGCCC CAACAATCTG
    AGCCCAACAG GCTGGTCACA GCCGAAAACC CCCGTGCCAG CACAAAGAGA GCGGGCACCA
    GTATCTGGGA CCCAGGAGAA AAACAAAATT AGGCCTCGTG GACAGAGAGA TTCAAGCTAT
    TATTGGGAAA TAGAAGCCAG TGAAGTGATG CTGTCCACTC GGATTGGGTC AGGCTCTTTT
    GGAACTGTTT ATAAGGGTAA ATGGCACGGA GATGTTGCAG TAAAGATCCT AAAGGTTGTC
    GACCCAACCC CAGAGCAATT CCAGGCCTTC AGGAATGAGG TGGCTGTTCT GCGCAAAACA
    CGGCATGTGA ACATTCTGCT TTTCATGGGG TACATGACAA AGGACAACCT GGCAATTGTG
    ACCCAGTGGT GCGAGGGCAG CAGCCTCTAC AAACACCTGC ATGTCCAGGA GACCAAGTTT
    CAGATGTTCC AGCTAATTGA CATTGCCCGG CAGACGGCTC AGGGAATGGA CTATTTGCAT
    GCAAAGAACA TCATCCATAG AGACATGAAA TCCAACAATA TATTTCTCCA TGAAGGCTTA
    ACAGTGAAAA TTGGAGATTT TGGTTTGGCA ACAGTAAAGT CACGCTGGAG TGGTTCTCAG
    CAGGTTGAAC AACCTACTGG CTCTGTCCTC TGGATGGCCC CAGAGGTGAT CCGAATGCAG
    GATAACAACC CATTCAGTTT CCAGTCGGAT GTCTACTCCT ATGGCATCGT ATTGTATGAA
    CTGATGACGG GGGAGCTTCC TTATTCTCAC ATCAACAACC GAGATCAGAT CATCTTCATG
    GTGGGCCGAG GATATGCCTC CCCAGATCTT AGTAAGCTAT ATAAGAACTG CCCCAAAGCA
    ATGAAGAGGC TGGTAGCTGA CTGTGTGAAG AAAGTAAAGG AAGAGAGGCC TCTTTTTCCC
    CAGATCCTGT CTTCCATTGA GCTGCTCCAA CACTCTCTAC CGAAGATCAA CCGGAGCGCT
    TCCGAGCCAT CCTTGCATCG GGCAGCCCAC ACTGAGGATA TCAATGCTTG CACGCTGACC
    ACGTCCCCGA GGCTGCCTGT CTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001341618.1
    CDS416..2422
    Translation

    Target ORF information:

    RefSeq Version NM_001354689.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001354689.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAGCACA TACAGGGAGC TTGGAAGACG ATCAGCAATG GTTTTGGATT CAAAGATGCC 
    GTGTTTGATG GCTCCAGCTG CATCTCTCCT ACAATAGTTC AGCAGTTTGG CTATCAGCGC
    CGGGCATCAG ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT
    TTCTTGCCGA ACAAGCAAAG AACAGTGGTC AATGTGCGAA ATGGAATGAG CTTGCATGAC
    TGCCTTATGA AAGCACTCAA GGTGAGGGGC CTGCAACCAG AGTGCTGTGC AGTGTTCAGA
    CTTCTCCACG AACACAAAGG TAAAAAAGCA CGCTTAGATT GGAATACTGA TGCTGCGTCT
    TTGATTGGAG AAGAACTTCA AGTAGATTTC CTGGATCATG TTCCCCTCAC AACACACAAC
    TTTGCTCGGA AGACGTTCCT GAAGCTTGCC TTCTGTGACA TCTGTCAGAA ATTCCTGCTC
    AATGGATTTC GATGTCAGAC TTGTGGCTAC AAATTTCATG AGCACTGTAG CACCAAAGTA
    CCTACTATGT GTGTGGACTG GAGTAACATC AGACAACTCT TATTGTTTCC AAATTCCACT
    ATTGGTGATA GTGGAGTCCC AGCACTACCT TCTTTGACTA TGCGTCGTAT GCGAGAGTCT
    GTTTCCAGGA TGCCTGTTAG TTCTCAGCAC AGATATTCTA CACCTCACGC CTTCACCTTT
    AACACCTCCA GTCCCTCATC TGAAGGTTCC CTCTCCCAGA GGCAGAGGTC GACATCCACA
    CCTAATGTCC ACATGGTCAG CACCACCCTG CCTGTGGACA GCAGGATGAT TGAGAATAAC
    AACCTGAGTG CTTCTCCCAG GGCGTGGTCC AGACGATTTT GTTTGAGGGG AAGAGATGCA
    ATTCGAAGTC ACAGCGAATC AGCCTCACCT TCAGCCCTGT CCAGTAGCCC CAACAATCTG
    AGCCCAACAG GCTGGTCACA GCCGAAAACC CCCGTGCCAG CACAAAGAGA GCGGGCACCA
    GTATCTGGGA CCCAGGAGAA AAACAAAATT AGGCCTCGTG GACAGAGAGA TTCAAGCTAT
    TATTGGGAAA TAGAAGCCAG TGAAGTGATG CTGTCCACTC GGATTGGGTC AGGCTCTTTT
    GGAACTGTTT ATAAGGGTAA ATGGCACGGA GATGTTGCAG TAAAGATCCT AAAGGTTGTC
    GACCCAACCC CAGAGCAATT CCAGGCCTTC AGGAATGAGG TGGCTGTTCT GCGCAAAACA
    CGGCATGTGA ACATTCTGCT TTTCATGGGG TACATGACAA AGGACAACCT GGCAATTGTG
    ACCCAGTGGT GCGAGGGCAG CAGCCTCTAC AAACACCTGC ATGTCCAGGA GACCAAGTTT
    CAGATGTTCC AGCTAATTGA CATTGCCCGG CAGACGGCTC AGGGAATGGA CTATTTGCAT
    GCAAAGAACA TCATCCATAG AGACATGAAA TCCAACAATA TATTTCTCCA TGAAGGCTTA
    ACAGTGAAAA TTGGAGATTT TGGTTTGGCA ACAGTAAAGT CACGCTGGAG TGGTTCTCAG
    CAGGTTGAAC AACCTACTGG CTCTGTCCTC TGGATGGCCC CAGAGGTGAT CCGAATGCAG
    GATAACAACC CATTCAGTTT CCAGTCGGAT GTCTACTCCT ATGGCATCGT ATTGTATGAA
    CTGATGACGG GGGAGCTTCC TTATTCTCAC ATCAACAACC GAGATCAGAT CATCTTCATG
    GTGGGCCGAG GATATGCCTC CCCAGATCTT AGTAAGCTAT ATAAGAACTG CCCCAAAGCA
    ATGAAGAGGC TGGTAGCTGA CTGTGTGAAG AAAGTAAAGG AAGAGAGGCC TCTTTTTCCC
    CAGATCCTGT CTTCCATTGA GCTGCTCCAA CACTCTCTAC CGAAGATCAA CCGGAGCGCT
    TCCGAGCCAT CCTTGCATCG GGCAGCCCAC ACTGAGGATA TCAATGCTTG CACGCTGACC
    ACGTCCCCGA GGCTGCCTGT CTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu103019
    Clone ID Related Accession (Same CDS sequence) NM_001354689.1 , NM_001354689.2 , NM_001354689.3
    Accession Version NM_001354689.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2007bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-06
    Organism Homo sapiens(human)
    Product RAF proto-oncogene serine/threonine-protein kinase isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC026170.5 and AC018500.4. On Jun 2, 2019 this sequence version replaced NM_001354689.1. Transcript Variant: This variant (1) encodes the longest isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.252894.1, SRR1660807.160683.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAGCACA TACAGGGAGC TTGGAAGACG ATCAGCAATG GTTTTGGATT CAAAGATGCC 
    GTGTTTGATG GCTCCAGCTG CATCTCTCCT ACAATAGTTC AGCAGTTTGG CTATCAGCGC
    CGGGCATCAG ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT
    TTCTTGCCGA ACAAGCAAAG AACAGTGGTC AATGTGCGAA ATGGAATGAG CTTGCATGAC
    TGCCTTATGA AAGCACTCAA GGTGAGGGGC CTGCAACCAG AGTGCTGTGC AGTGTTCAGA
    CTTCTCCACG AACACAAAGG TAAAAAAGCA CGCTTAGATT GGAATACTGA TGCTGCGTCT
    TTGATTGGAG AAGAACTTCA AGTAGATTTC CTGGATCATG TTCCCCTCAC AACACACAAC
    TTTGCTCGGA AGACGTTCCT GAAGCTTGCC TTCTGTGACA TCTGTCAGAA ATTCCTGCTC
    AATGGATTTC GATGTCAGAC TTGTGGCTAC AAATTTCATG AGCACTGTAG CACCAAAGTA
    CCTACTATGT GTGTGGACTG GAGTAACATC AGACAACTCT TATTGTTTCC AAATTCCACT
    ATTGGTGATA GTGGAGTCCC AGCACTACCT TCTTTGACTA TGCGTCGTAT GCGAGAGTCT
    GTTTCCAGGA TGCCTGTTAG TTCTCAGCAC AGATATTCTA CACCTCACGC CTTCACCTTT
    AACACCTCCA GTCCCTCATC TGAAGGTTCC CTCTCCCAGA GGCAGAGGTC GACATCCACA
    CCTAATGTCC ACATGGTCAG CACCACCCTG CCTGTGGACA GCAGGATGAT TGAGAATAAC
    AACCTGAGTG CTTCTCCCAG GGCGTGGTCC AGACGATTTT GTTTGAGGGG AAGAGATGCA
    ATTCGAAGTC ACAGCGAATC AGCCTCACCT TCAGCCCTGT CCAGTAGCCC CAACAATCTG
    AGCCCAACAG GCTGGTCACA GCCGAAAACC CCCGTGCCAG CACAAAGAGA GCGGGCACCA
    GTATCTGGGA CCCAGGAGAA AAACAAAATT AGGCCTCGTG GACAGAGAGA TTCAAGCTAT
    TATTGGGAAA TAGAAGCCAG TGAAGTGATG CTGTCCACTC GGATTGGGTC AGGCTCTTTT
    GGAACTGTTT ATAAGGGTAA ATGGCACGGA GATGTTGCAG TAAAGATCCT AAAGGTTGTC
    GACCCAACCC CAGAGCAATT CCAGGCCTTC AGGAATGAGG TGGCTGTTCT GCGCAAAACA
    CGGCATGTGA ACATTCTGCT TTTCATGGGG TACATGACAA AGGACAACCT GGCAATTGTG
    ACCCAGTGGT GCGAGGGCAG CAGCCTCTAC AAACACCTGC ATGTCCAGGA GACCAAGTTT
    CAGATGTTCC AGCTAATTGA CATTGCCCGG CAGACGGCTC AGGGAATGGA CTATTTGCAT
    GCAAAGAACA TCATCCATAG AGACATGAAA TCCAACAATA TATTTCTCCA TGAAGGCTTA
    ACAGTGAAAA TTGGAGATTT TGGTTTGGCA ACAGTAAAGT CACGCTGGAG TGGTTCTCAG
    CAGGTTGAAC AACCTACTGG CTCTGTCCTC TGGATGGCCC CAGAGGTGAT CCGAATGCAG
    GATAACAACC CATTCAGTTT CCAGTCGGAT GTCTACTCCT ATGGCATCGT ATTGTATGAA
    CTGATGACGG GGGAGCTTCC TTATTCTCAC ATCAACAACC GAGATCAGAT CATCTTCATG
    GTGGGCCGAG GATATGCCTC CCCAGATCTT AGTAAGCTAT ATAAGAACTG CCCCAAAGCA
    ATGAAGAGGC TGGTAGCTGA CTGTGTGAAG AAAGTAAAGG AAGAGAGGCC TCTTTTTCCC
    CAGATCCTGT CTTCCATTGA GCTGCTCCAA CACTCTCTAC CGAAGATCAA CCGGAGCGCT
    TCCGAGCCAT CCTTGCATCG GGCAGCCCAC ACTGAGGATA TCAATGCTTG CACGCTGACC
    ACGTCCCCGA GGCTGCCTGT CTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001341618.1
    CDS332..2338
    Translation

    Target ORF information:

    RefSeq Version NM_001354689.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001354689.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAGCACA TACAGGGAGC TTGGAAGACG ATCAGCAATG GTTTTGGATT CAAAGATGCC 
    GTGTTTGATG GCTCCAGCTG CATCTCTCCT ACAATAGTTC AGCAGTTTGG CTATCAGCGC
    CGGGCATCAG ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT
    TTCTTGCCGA ACAAGCAAAG AACAGTGGTC AATGTGCGAA ATGGAATGAG CTTGCATGAC
    TGCCTTATGA AAGCACTCAA GGTGAGGGGC CTGCAACCAG AGTGCTGTGC AGTGTTCAGA
    CTTCTCCACG AACACAAAGG TAAAAAAGCA CGCTTAGATT GGAATACTGA TGCTGCGTCT
    TTGATTGGAG AAGAACTTCA AGTAGATTTC CTGGATCATG TTCCCCTCAC AACACACAAC
    TTTGCTCGGA AGACGTTCCT GAAGCTTGCC TTCTGTGACA TCTGTCAGAA ATTCCTGCTC
    AATGGATTTC GATGTCAGAC TTGTGGCTAC AAATTTCATG AGCACTGTAG CACCAAAGTA
    CCTACTATGT GTGTGGACTG GAGTAACATC AGACAACTCT TATTGTTTCC AAATTCCACT
    ATTGGTGATA GTGGAGTCCC AGCACTACCT TCTTTGACTA TGCGTCGTAT GCGAGAGTCT
    GTTTCCAGGA TGCCTGTTAG TTCTCAGCAC AGATATTCTA CACCTCACGC CTTCACCTTT
    AACACCTCCA GTCCCTCATC TGAAGGTTCC CTCTCCCAGA GGCAGAGGTC GACATCCACA
    CCTAATGTCC ACATGGTCAG CACCACCCTG CCTGTGGACA GCAGGATGAT TGAGAATAAC
    AACCTGAGTG CTTCTCCCAG GGCGTGGTCC AGACGATTTT GTTTGAGGGG AAGAGATGCA
    ATTCGAAGTC ACAGCGAATC AGCCTCACCT TCAGCCCTGT CCAGTAGCCC CAACAATCTG
    AGCCCAACAG GCTGGTCACA GCCGAAAACC CCCGTGCCAG CACAAAGAGA GCGGGCACCA
    GTATCTGGGA CCCAGGAGAA AAACAAAATT AGGCCTCGTG GACAGAGAGA TTCAAGCTAT
    TATTGGGAAA TAGAAGCCAG TGAAGTGATG CTGTCCACTC GGATTGGGTC AGGCTCTTTT
    GGAACTGTTT ATAAGGGTAA ATGGCACGGA GATGTTGCAG TAAAGATCCT AAAGGTTGTC
    GACCCAACCC CAGAGCAATT CCAGGCCTTC AGGAATGAGG TGGCTGTTCT GCGCAAAACA
    CGGCATGTGA ACATTCTGCT TTTCATGGGG TACATGACAA AGGACAACCT GGCAATTGTG
    ACCCAGTGGT GCGAGGGCAG CAGCCTCTAC AAACACCTGC ATGTCCAGGA GACCAAGTTT
    CAGATGTTCC AGCTAATTGA CATTGCCCGG CAGACGGCTC AGGGAATGGA CTATTTGCAT
    GCAAAGAACA TCATCCATAG AGACATGAAA TCCAACAATA TATTTCTCCA TGAAGGCTTA
    ACAGTGAAAA TTGGAGATTT TGGTTTGGCA ACAGTAAAGT CACGCTGGAG TGGTTCTCAG
    CAGGTTGAAC AACCTACTGG CTCTGTCCTC TGGATGGCCC CAGAGGTGAT CCGAATGCAG
    GATAACAACC CATTCAGTTT CCAGTCGGAT GTCTACTCCT ATGGCATCGT ATTGTATGAA
    CTGATGACGG GGGAGCTTCC TTATTCTCAC ATCAACAACC GAGATCAGAT CATCTTCATG
    GTGGGCCGAG GATATGCCTC CCCAGATCTT AGTAAGCTAT ATAAGAACTG CCCCAAAGCA
    ATGAAGAGGC TGGTAGCTGA CTGTGTGAAG AAAGTAAAGG AAGAGAGGCC TCTTTTTCCC
    CAGATCCTGT CTTCCATTGA GCTGCTCCAA CACTCTCTAC CGAAGATCAA CCGGAGCGCT
    TCCGAGCCAT CCTTGCATCG GGCAGCCCAC ACTGAGGATA TCAATGCTTG CACGCTGACC
    ACGTCCCCGA GGCTGCCTGT CTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu103019
    Clone ID Related Accession (Same CDS sequence) NM_001354689.1 , NM_001354689.2 , NM_001354689.3
    Accession Version NM_001354689.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2007bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-07
    Organism Homo sapiens(human)
    Product RAF proto-oncogene serine/threonine-protein kinase isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC026170.5 and AC018500.4. This sequence is a reference standard in the RefSeqGene project. On Jul 15, 2019 this sequence version replaced NM_001354689.2. Transcript Variant: This variant (1) encodes the longest isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.252894.1, SRR1660807.160683.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAGCACA TACAGGGAGC TTGGAAGACG ATCAGCAATG GTTTTGGATT CAAAGATGCC 
    GTGTTTGATG GCTCCAGCTG CATCTCTCCT ACAATAGTTC AGCAGTTTGG CTATCAGCGC
    CGGGCATCAG ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT
    TTCTTGCCGA ACAAGCAAAG AACAGTGGTC AATGTGCGAA ATGGAATGAG CTTGCATGAC
    TGCCTTATGA AAGCACTCAA GGTGAGGGGC CTGCAACCAG AGTGCTGTGC AGTGTTCAGA
    CTTCTCCACG AACACAAAGG TAAAAAAGCA CGCTTAGATT GGAATACTGA TGCTGCGTCT
    TTGATTGGAG AAGAACTTCA AGTAGATTTC CTGGATCATG TTCCCCTCAC AACACACAAC
    TTTGCTCGGA AGACGTTCCT GAAGCTTGCC TTCTGTGACA TCTGTCAGAA ATTCCTGCTC
    AATGGATTTC GATGTCAGAC TTGTGGCTAC AAATTTCATG AGCACTGTAG CACCAAAGTA
    CCTACTATGT GTGTGGACTG GAGTAACATC AGACAACTCT TATTGTTTCC AAATTCCACT
    ATTGGTGATA GTGGAGTCCC AGCACTACCT TCTTTGACTA TGCGTCGTAT GCGAGAGTCT
    GTTTCCAGGA TGCCTGTTAG TTCTCAGCAC AGATATTCTA CACCTCACGC CTTCACCTTT
    AACACCTCCA GTCCCTCATC TGAAGGTTCC CTCTCCCAGA GGCAGAGGTC GACATCCACA
    CCTAATGTCC ACATGGTCAG CACCACCCTG CCTGTGGACA GCAGGATGAT TGAGAATAAC
    AACCTGAGTG CTTCTCCCAG GGCGTGGTCC AGACGATTTT GTTTGAGGGG AAGAGATGCA
    ATTCGAAGTC ACAGCGAATC AGCCTCACCT TCAGCCCTGT CCAGTAGCCC CAACAATCTG
    AGCCCAACAG GCTGGTCACA GCCGAAAACC CCCGTGCCAG CACAAAGAGA GCGGGCACCA
    GTATCTGGGA CCCAGGAGAA AAACAAAATT AGGCCTCGTG GACAGAGAGA TTCAAGCTAT
    TATTGGGAAA TAGAAGCCAG TGAAGTGATG CTGTCCACTC GGATTGGGTC AGGCTCTTTT
    GGAACTGTTT ATAAGGGTAA ATGGCACGGA GATGTTGCAG TAAAGATCCT AAAGGTTGTC
    GACCCAACCC CAGAGCAATT CCAGGCCTTC AGGAATGAGG TGGCTGTTCT GCGCAAAACA
    CGGCATGTGA ACATTCTGCT TTTCATGGGG TACATGACAA AGGACAACCT GGCAATTGTG
    ACCCAGTGGT GCGAGGGCAG CAGCCTCTAC AAACACCTGC ATGTCCAGGA GACCAAGTTT
    CAGATGTTCC AGCTAATTGA CATTGCCCGG CAGACGGCTC AGGGAATGGA CTATTTGCAT
    GCAAAGAACA TCATCCATAG AGACATGAAA TCCAACAATA TATTTCTCCA TGAAGGCTTA
    ACAGTGAAAA TTGGAGATTT TGGTTTGGCA ACAGTAAAGT CACGCTGGAG TGGTTCTCAG
    CAGGTTGAAC AACCTACTGG CTCTGTCCTC TGGATGGCCC CAGAGGTGAT CCGAATGCAG
    GATAACAACC CATTCAGTTT CCAGTCGGAT GTCTACTCCT ATGGCATCGT ATTGTATGAA
    CTGATGACGG GGGAGCTTCC TTATTCTCAC ATCAACAACC GAGATCAGAT CATCTTCATG
    GTGGGCCGAG GATATGCCTC CCCAGATCTT AGTAAGCTAT ATAAGAACTG CCCCAAAGCA
    ATGAAGAGGC TGGTAGCTGA CTGTGTGAAG AAAGTAAAGG AAGAGAGGCC TCTTTTTCCC
    CAGATCCTGT CTTCCATTGA GCTGCTCCAA CACTCTCTAC CGAAGATCAA CCGGAGCGCT
    TCCGAGCCAT CCTTGCATCG GGCAGCCCAC ACTGAGGATA TCAATGCTTG CACGCTGACC
    ACGTCCCCGA GGCTGCCTGT CTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001341618.1
    CDS332..2338
    Translation

    Target ORF information:

    RefSeq Version NM_001354689.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001354689.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAGCACA TACAGGGAGC TTGGAAGACG ATCAGCAATG GTTTTGGATT CAAAGATGCC 
    GTGTTTGATG GCTCCAGCTG CATCTCTCCT ACAATAGTTC AGCAGTTTGG CTATCAGCGC
    CGGGCATCAG ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT
    TTCTTGCCGA ACAAGCAAAG AACAGTGGTC AATGTGCGAA ATGGAATGAG CTTGCATGAC
    TGCCTTATGA AAGCACTCAA GGTGAGGGGC CTGCAACCAG AGTGCTGTGC AGTGTTCAGA
    CTTCTCCACG AACACAAAGG TAAAAAAGCA CGCTTAGATT GGAATACTGA TGCTGCGTCT
    TTGATTGGAG AAGAACTTCA AGTAGATTTC CTGGATCATG TTCCCCTCAC AACACACAAC
    TTTGCTCGGA AGACGTTCCT GAAGCTTGCC TTCTGTGACA TCTGTCAGAA ATTCCTGCTC
    AATGGATTTC GATGTCAGAC TTGTGGCTAC AAATTTCATG AGCACTGTAG CACCAAAGTA
    CCTACTATGT GTGTGGACTG GAGTAACATC AGACAACTCT TATTGTTTCC AAATTCCACT
    ATTGGTGATA GTGGAGTCCC AGCACTACCT TCTTTGACTA TGCGTCGTAT GCGAGAGTCT
    GTTTCCAGGA TGCCTGTTAG TTCTCAGCAC AGATATTCTA CACCTCACGC CTTCACCTTT
    AACACCTCCA GTCCCTCATC TGAAGGTTCC CTCTCCCAGA GGCAGAGGTC GACATCCACA
    CCTAATGTCC ACATGGTCAG CACCACCCTG CCTGTGGACA GCAGGATGAT TGAGAATAAC
    AACCTGAGTG CTTCTCCCAG GGCGTGGTCC AGACGATTTT GTTTGAGGGG AAGAGATGCA
    ATTCGAAGTC ACAGCGAATC AGCCTCACCT TCAGCCCTGT CCAGTAGCCC CAACAATCTG
    AGCCCAACAG GCTGGTCACA GCCGAAAACC CCCGTGCCAG CACAAAGAGA GCGGGCACCA
    GTATCTGGGA CCCAGGAGAA AAACAAAATT AGGCCTCGTG GACAGAGAGA TTCAAGCTAT
    TATTGGGAAA TAGAAGCCAG TGAAGTGATG CTGTCCACTC GGATTGGGTC AGGCTCTTTT
    GGAACTGTTT ATAAGGGTAA ATGGCACGGA GATGTTGCAG TAAAGATCCT AAAGGTTGTC
    GACCCAACCC CAGAGCAATT CCAGGCCTTC AGGAATGAGG TGGCTGTTCT GCGCAAAACA
    CGGCATGTGA ACATTCTGCT TTTCATGGGG TACATGACAA AGGACAACCT GGCAATTGTG
    ACCCAGTGGT GCGAGGGCAG CAGCCTCTAC AAACACCTGC ATGTCCAGGA GACCAAGTTT
    CAGATGTTCC AGCTAATTGA CATTGCCCGG CAGACGGCTC AGGGAATGGA CTATTTGCAT
    GCAAAGAACA TCATCCATAG AGACATGAAA TCCAACAATA TATTTCTCCA TGAAGGCTTA
    ACAGTGAAAA TTGGAGATTT TGGTTTGGCA ACAGTAAAGT CACGCTGGAG TGGTTCTCAG
    CAGGTTGAAC AACCTACTGG CTCTGTCCTC TGGATGGCCC CAGAGGTGAT CCGAATGCAG
    GATAACAACC CATTCAGTTT CCAGTCGGAT GTCTACTCCT ATGGCATCGT ATTGTATGAA
    CTGATGACGG GGGAGCTTCC TTATTCTCAC ATCAACAACC GAGATCAGAT CATCTTCATG
    GTGGGCCGAG GATATGCCTC CCCAGATCTT AGTAAGCTAT ATAAGAACTG CCCCAAAGCA
    ATGAAGAGGC TGGTAGCTGA CTGTGTGAAG AAAGTAAAGG AAGAGAGGCC TCTTTTTCCC
    CAGATCCTGT CTTCCATTGA GCTGCTCCAA CACTCTCTAC CGAAGATCAA CCGGAGCGCT
    TCCGAGCCAT CCTTGCATCG GGCAGCCCAC ACTGAGGATA TCAATGCTTG CACGCTGACC
    ACGTCCCCGA GGCTGCCTGT CTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu103097
    Clone ID Related Accession (Same CDS sequence) NM_001354694.2 , NM_001354694.1
    Accession Version NM_001354694.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1764bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-07
    Organism Homo sapiens(human)
    Product RAF proto-oncogene serine/threonine-protein kinase isoform e
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC026170.5 and AC018500.4. On Jun 2, 2019 this sequence version replaced NM_001354694.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.74921.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT TTCTTGCCGA 
    ACAAGCAAAG AACAGTGTAA AAAAGCACGC TTAGATTGGA ATACTGATGC TGCGTCTTTG
    ATTGGAGAAG AACTTCAAGT AGATTTCCTG GATCATGTTC CCCTCACAAC ACACAACTTT
    GCTCGGAAGA CGTTCCTGAA GCTTGCCTTC TGTGACATCT GTCAGAAATT CCTGCTCAAT
    GGATTTCGAT GTCAGACTTG TGGCTACAAA TTTCATGAGC ACTGTAGCAC CAAAGTACCT
    ACTATGTGTG TGGACTGGAG TAACATCAGA CAACTCTTAT TGTTTCCAAA TTCCACTATT
    GGTGATAGTG GAGTCCCAGC ACTACCTTCT TTGACTATGC GTCGTATGCG AGAGTCTGTT
    TCCAGGATGC CTGTTAGTTC TCAGCACAGA TATTCTACAC CTCACGCCTT CACCTTTAAC
    ACCTCCAGTC CCTCATCTGA AGGTTCCCTC TCCCAGAGGC AGAGGTCGAC ATCCACACCT
    AATGTCCACA TGGTCAGCAC CACCCTGCCT GTGGACAGCA GGATGATTGA GAATAACAAC
    CTGAGTGCTT CTCCCAGGGC GTGGTCCAGA CGATTTTGTT TGAGGGGAAG AGATGCAATT
    CGAAGTCACA GCGAATCAGC CTCACCTTCA GCCCTGTCCA GTAGCCCCAA CAATCTGAGC
    CCAACAGGCT GGTCACAGCC GAAAACCCCC GTGCCAGCAC AAAGAGAGCG GGCACCAGTA
    TCTGGGACCC AGGAGAAAAA CAAAATTAGG CCTCGTGGAC AGAGAGATTC AAGCTATTAT
    TGGGAAATAG AAGCCAGTGA AGTGATGCTG TCCACTCGGA TTGGGTCAGG CTCTTTTGGA
    ACTGTTTATA AGGGTAAATG GCACGGAGAT GTTGCAGTAA AGATCCTAAA GGTTGTCGAC
    CCAACCCCAG AGCAATTCCA GGCCTTCAGG AATGAGGTGG CTGTTCTGCG CAAAACACGG
    CATGTGAACA TTCTGCTTTT CATGGGGTAC ATGACAAAGG ACAACCTGGC AATTGTGACC
    CAGTGGTGCG AGGGCAGCAG CCTCTACAAA CACCTGCATG TCCAGGAGAC CAAGTTTCAG
    ATGTTCCAGC TAATTGACAT TGCCCGGCAG ACGGCTCAGG GAATGGACTA TTTGCATGCA
    AAGAACATCA TCCATAGAGA CATGAAATCC AACAATATAT TTCTCCATGA AGGCTTAACA
    GTGAAAATTG GAGATTTTGG TTTGGCAACA GTAAAGTCAC GCTGGAGTGG TTCTCAGCAG
    GTTGAACAAC CTACTGGCTC TGTCCTCTGG ATGGCCCCAG AGGTGATCCG AATGCAGGAT
    AACAACCCAT TCAGTTTCCA GTCGGATGTC TACTCCTATG GCATCGTATT GTATGAACTG
    ATGACGGGGG AGCTTCCTTA TTCTCACATC AACAACCGAG ATCAGATCAT CTTCATGGTG
    GGCCGAGGAT ATGCCTCCCC AGATCTTAGT AAGCTATATA AGAACTGCCC CAAAGCAATG
    AAGAGGCTGG TAGCTGACTG TGTGAAGAAA GTAAAGGAAG AGAGGCCTCT TTTTCCCCAG
    ATCCTGTCTT CCATTGAGCT GCTCCAACAC TCTCTACCGA AGATCAACCG GAGCGCTTCC
    GAGCCATCCT TGCATCGGGC AGCCCACACT GAGGATATCA ATGCTTGCAC GCTGACCACG
    TCCCCGAGGC TGCCTGTCTT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001341623.1
    CDS462..2225
    Translation

    Target ORF information:

    RefSeq Version NM_001354694.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001354694.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT TTCTTGCCGA 
    ACAAGCAAAG AACAGTGTAA AAAAGCACGC TTAGATTGGA ATACTGATGC TGCGTCTTTG
    ATTGGAGAAG AACTTCAAGT AGATTTCCTG GATCATGTTC CCCTCACAAC ACACAACTTT
    GCTCGGAAGA CGTTCCTGAA GCTTGCCTTC TGTGACATCT GTCAGAAATT CCTGCTCAAT
    GGATTTCGAT GTCAGACTTG TGGCTACAAA TTTCATGAGC ACTGTAGCAC CAAAGTACCT
    ACTATGTGTG TGGACTGGAG TAACATCAGA CAACTCTTAT TGTTTCCAAA TTCCACTATT
    GGTGATAGTG GAGTCCCAGC ACTACCTTCT TTGACTATGC GTCGTATGCG AGAGTCTGTT
    TCCAGGATGC CTGTTAGTTC TCAGCACAGA TATTCTACAC CTCACGCCTT CACCTTTAAC
    ACCTCCAGTC CCTCATCTGA AGGTTCCCTC TCCCAGAGGC AGAGGTCGAC ATCCACACCT
    AATGTCCACA TGGTCAGCAC CACCCTGCCT GTGGACAGCA GGATGATTGA GAATAACAAC
    CTGAGTGCTT CTCCCAGGGC GTGGTCCAGA CGATTTTGTT TGAGGGGAAG AGATGCAATT
    CGAAGTCACA GCGAATCAGC CTCACCTTCA GCCCTGTCCA GTAGCCCCAA CAATCTGAGC
    CCAACAGGCT GGTCACAGCC GAAAACCCCC GTGCCAGCAC AAAGAGAGCG GGCACCAGTA
    TCTGGGACCC AGGAGAAAAA CAAAATTAGG CCTCGTGGAC AGAGAGATTC AAGCTATTAT
    TGGGAAATAG AAGCCAGTGA AGTGATGCTG TCCACTCGGA TTGGGTCAGG CTCTTTTGGA
    ACTGTTTATA AGGGTAAATG GCACGGAGAT GTTGCAGTAA AGATCCTAAA GGTTGTCGAC
    CCAACCCCAG AGCAATTCCA GGCCTTCAGG AATGAGGTGG CTGTTCTGCG CAAAACACGG
    CATGTGAACA TTCTGCTTTT CATGGGGTAC ATGACAAAGG ACAACCTGGC AATTGTGACC
    CAGTGGTGCG AGGGCAGCAG CCTCTACAAA CACCTGCATG TCCAGGAGAC CAAGTTTCAG
    ATGTTCCAGC TAATTGACAT TGCCCGGCAG ACGGCTCAGG GAATGGACTA TTTGCATGCA
    AAGAACATCA TCCATAGAGA CATGAAATCC AACAATATAT TTCTCCATGA AGGCTTAACA
    GTGAAAATTG GAGATTTTGG TTTGGCAACA GTAAAGTCAC GCTGGAGTGG TTCTCAGCAG
    GTTGAACAAC CTACTGGCTC TGTCCTCTGG ATGGCCCCAG AGGTGATCCG AATGCAGGAT
    AACAACCCAT TCAGTTTCCA GTCGGATGTC TACTCCTATG GCATCGTATT GTATGAACTG
    ATGACGGGGG AGCTTCCTTA TTCTCACATC AACAACCGAG ATCAGATCAT CTTCATGGTG
    GGCCGAGGAT ATGCCTCCCC AGATCTTAGT AAGCTATATA AGAACTGCCC CAAAGCAATG
    AAGAGGCTGG TAGCTGACTG TGTGAAGAAA GTAAAGGAAG AGAGGCCTCT TTTTCCCCAG
    ATCCTGTCTT CCATTGAGCT GCTCCAACAC TCTCTACCGA AGATCAACCG GAGCGCTTCC
    GAGCCATCCT TGCATCGGGC AGCCCACACT GAGGATATCA ATGCTTGCAC GCTGACCACG
    TCCCCGAGGC TGCCTGTCTT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu103097
    Clone ID Related Accession (Same CDS sequence) NM_001354694.2 , NM_001354694.1
    Accession Version NM_001354694.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1764bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product RAF proto-oncogene serine/threonine-protein kinase isoform e
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC026170.5 and AC018500.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.74921.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT TTCTTGCCGA 
    ACAAGCAAAG AACAGTGTAA AAAAGCACGC TTAGATTGGA ATACTGATGC TGCGTCTTTG
    ATTGGAGAAG AACTTCAAGT AGATTTCCTG GATCATGTTC CCCTCACAAC ACACAACTTT
    GCTCGGAAGA CGTTCCTGAA GCTTGCCTTC TGTGACATCT GTCAGAAATT CCTGCTCAAT
    GGATTTCGAT GTCAGACTTG TGGCTACAAA TTTCATGAGC ACTGTAGCAC CAAAGTACCT
    ACTATGTGTG TGGACTGGAG TAACATCAGA CAACTCTTAT TGTTTCCAAA TTCCACTATT
    GGTGATAGTG GAGTCCCAGC ACTACCTTCT TTGACTATGC GTCGTATGCG AGAGTCTGTT
    TCCAGGATGC CTGTTAGTTC TCAGCACAGA TATTCTACAC CTCACGCCTT CACCTTTAAC
    ACCTCCAGTC CCTCATCTGA AGGTTCCCTC TCCCAGAGGC AGAGGTCGAC ATCCACACCT
    AATGTCCACA TGGTCAGCAC CACCCTGCCT GTGGACAGCA GGATGATTGA GAATAACAAC
    CTGAGTGCTT CTCCCAGGGC GTGGTCCAGA CGATTTTGTT TGAGGGGAAG AGATGCAATT
    CGAAGTCACA GCGAATCAGC CTCACCTTCA GCCCTGTCCA GTAGCCCCAA CAATCTGAGC
    CCAACAGGCT GGTCACAGCC GAAAACCCCC GTGCCAGCAC AAAGAGAGCG GGCACCAGTA
    TCTGGGACCC AGGAGAAAAA CAAAATTAGG CCTCGTGGAC AGAGAGATTC AAGCTATTAT
    TGGGAAATAG AAGCCAGTGA AGTGATGCTG TCCACTCGGA TTGGGTCAGG CTCTTTTGGA
    ACTGTTTATA AGGGTAAATG GCACGGAGAT GTTGCAGTAA AGATCCTAAA GGTTGTCGAC
    CCAACCCCAG AGCAATTCCA GGCCTTCAGG AATGAGGTGG CTGTTCTGCG CAAAACACGG
    CATGTGAACA TTCTGCTTTT CATGGGGTAC ATGACAAAGG ACAACCTGGC AATTGTGACC
    CAGTGGTGCG AGGGCAGCAG CCTCTACAAA CACCTGCATG TCCAGGAGAC CAAGTTTCAG
    ATGTTCCAGC TAATTGACAT TGCCCGGCAG ACGGCTCAGG GAATGGACTA TTTGCATGCA
    AAGAACATCA TCCATAGAGA CATGAAATCC AACAATATAT TTCTCCATGA AGGCTTAACA
    GTGAAAATTG GAGATTTTGG TTTGGCAACA GTAAAGTCAC GCTGGAGTGG TTCTCAGCAG
    GTTGAACAAC CTACTGGCTC TGTCCTCTGG ATGGCCCCAG AGGTGATCCG AATGCAGGAT
    AACAACCCAT TCAGTTTCCA GTCGGATGTC TACTCCTATG GCATCGTATT GTATGAACTG
    ATGACGGGGG AGCTTCCTTA TTCTCACATC AACAACCGAG ATCAGATCAT CTTCATGGTG
    GGCCGAGGAT ATGCCTCCCC AGATCTTAGT AAGCTATATA AGAACTGCCC CAAAGCAATG
    AAGAGGCTGG TAGCTGACTG TGTGAAGAAA GTAAAGGAAG AGAGGCCTCT TTTTCCCCAG
    ATCCTGTCTT CCATTGAGCT GCTCCAACAC TCTCTACCGA AGATCAACCG GAGCGCTTCC
    GAGCCATCCT TGCATCGGGC AGCCCACACT GAGGATATCA ATGCTTGCAC GCTGACCACG
    TCCCCGAGGC TGCCTGTCTT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001341623.1
    CDS546..2309
    Translation

    Target ORF information:

    RefSeq Version NM_001354694.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001354694.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC TATCCGTGTT TTCTTGCCGA 
    ACAAGCAAAG AACAGTGTAA AAAAGCACGC TTAGATTGGA ATACTGATGC TGCGTCTTTG
    ATTGGAGAAG AACTTCAAGT AGATTTCCTG GATCATGTTC CCCTCACAAC ACACAACTTT
    GCTCGGAAGA CGTTCCTGAA GCTTGCCTTC TGTGACATCT GTCAGAAATT CCTGCTCAAT
    GGATTTCGAT GTCAGACTTG TGGCTACAAA TTTCATGAGC ACTGTAGCAC CAAAGTACCT
    ACTATGTGTG TGGACTGGAG TAACATCAGA CAACTCTTAT TGTTTCCAAA TTCCACTATT
    GGTGATAGTG GAGTCCCAGC ACTACCTTCT TTGACTATGC GTCGTATGCG AGAGTCTGTT
    TCCAGGATGC CTGTTAGTTC TCAGCACAGA TATTCTACAC CTCACGCCTT CACCTTTAAC
    ACCTCCAGTC CCTCATCTGA AGGTTCCCTC TCCCAGAGGC AGAGGTCGAC ATCCACACCT
    AATGTCCACA TGGTCAGCAC CACCCTGCCT GTGGACAGCA GGATGATTGA GAATAACAAC
    CTGAGTGCTT CTCCCAGGGC GTGGTCCAGA CGATTTTGTT TGAGGGGAAG AGATGCAATT
    CGAAGTCACA GCGAATCAGC CTCACCTTCA GCCCTGTCCA GTAGCCCCAA CAATCTGAGC
    CCAACAGGCT GGTCACAGCC GAAAACCCCC GTGCCAGCAC AAAGAGAGCG GGCACCAGTA
    TCTGGGACCC AGGAGAAAAA CAAAATTAGG CCTCGTGGAC AGAGAGATTC AAGCTATTAT
    TGGGAAATAG AAGCCAGTGA AGTGATGCTG TCCACTCGGA TTGGGTCAGG CTCTTTTGGA
    ACTGTTTATA AGGGTAAATG GCACGGAGAT GTTGCAGTAA AGATCCTAAA GGTTGTCGAC
    CCAACCCCAG AGCAATTCCA GGCCTTCAGG AATGAGGTGG CTGTTCTGCG CAAAACACGG
    CATGTGAACA TTCTGCTTTT CATGGGGTAC ATGACAAAGG ACAACCTGGC AATTGTGACC
    CAGTGGTGCG AGGGCAGCAG CCTCTACAAA CACCTGCATG TCCAGGAGAC CAAGTTTCAG
    ATGTTCCAGC TAATTGACAT TGCCCGGCAG ACGGCTCAGG GAATGGACTA TTTGCATGCA
    AAGAACATCA TCCATAGAGA CATGAAATCC AACAATATAT TTCTCCATGA AGGCTTAACA
    GTGAAAATTG GAGATTTTGG TTTGGCAACA GTAAAGTCAC GCTGGAGTGG TTCTCAGCAG
    GTTGAACAAC CTACTGGCTC TGTCCTCTGG ATGGCCCCAG AGGTGATCCG AATGCAGGAT
    AACAACCCAT TCAGTTTCCA GTCGGATGTC TACTCCTATG GCATCGTATT GTATGAACTG
    ATGACGGGGG AGCTTCCTTA TTCTCACATC AACAACCGAG ATCAGATCAT CTTCATGGTG
    GGCCGAGGAT ATGCCTCCCC AGATCTTAGT AAGCTATATA AGAACTGCCC CAAAGCAATG
    AAGAGGCTGG TAGCTGACTG TGTGAAGAAA GTAAAGGAAG AGAGGCCTCT TTTTCCCCAG
    ATCCTGTCTT CCATTGAGCT GCTCCAACAC TCTCTACCGA AGATCAACCG GAGCGCTTCC
    GAGCCATCCT TGCATCGGGC AGCCCACACT GAGGATATCA ATGCTTGCAC GCTGACCACG
    TCCCCGAGGC TGCCTGTCTT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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