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CCL2 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol CCL2
Entrez Gene ID 6347
Full Name C-C motif chemokine ligand 2
Synonyms GDCF-2,HC11,HSMCR30,MCAF,MCP-1,MCP1,SCYA2,SMC-CF
General protein information
Preferred Names

C-C motif chemokine ligand 2

Names

C-C motif chemokine 2
chemokine (C-C motif) ligand 2
monocyte chemoattractant protein-1
monocyte chemotactic and activating factor
monocyte chemotactic protein 1
monocyte secretory protein JE
small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je)
small inducible cytokine subfamily A (Cys-Cys), member 2
small-inducible cytokine A2

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

17

17q12

Summary This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2013].
Disorder MIM:

158105

Disorder Html: {HIV-1, resistance to}, 609423 (3); {Spina bifida, susceptibility

mRNA and Protein(s)

mRNA Protein Name
NM_002982.3 NP_002973.1 C-C motif chemokine 2 precursor



Pan troglodytes (chimpanzee) CCL2 XP_001174551.1
Bos taurus (cattle) CCL2 NP_776431.1
Canis lupus familiaris (dog) CCL2 NP_001003297.1
Macaca mulatta (rhesus monkey) CCL2 NP_001027993.1
Homo sapiens (human) CCL2 NP_002973.1
Mus musculus (house mouse) Ccl12 NP_035461.2
Rattus norvegicus (Norway rat) Ccl12 NP_001099292.1


Related articles in PubMed

CCL2 expression correlates with Snail expression and affects the prognosis of patients with gastric cancer.
Zhang J, Yan Y, Cui X, Zhang J, Yang Y, Li H, Wu H, Li J, Wang L, Li M, Liu X, Wang J, Duan X
Pathology, research and practice213(3)217-221(2017 Mar)

Specific tumor-derived CCL2 mediated by pyruvate kinase M2 in colorectal cancer cells contributes to macrophage recruitment in tumor microenvironment.
Zou K, Wang Y, Hu Y, Zheng L, Xu W, Li G
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine39(3)1010428317695962(2017 Mar)

Plasma Monocyte Chemoattractant Protein 1 as a Predictive Marker for Sepsis Prognosis: A Prospective Cohort Study.
Zhu T, Liao X, Feng T, Wu Q, Zhang J, Cao X, Li H
The Tohoku journal of experimental medicine241(2)139-147(2017 02)

Urine Epidermal Growth Factor, Monocyte Chemoattractant Protein-1 or Their Ratio as Biomarkers for Interstitial Fibrosis and Tubular Atrophy in Primary Glomerulonephritis.
Worawichawong S, Worawichawong S, Radinahamed P, Muntham D, Sathirapongsasuti N, Nongnuch A, Assanatham M, Kitiyakara C
Kidney & blood pressure research41(6)997-1007(2016)

Profiles of Inflammatory Cytokines in the Vitreous Fluid from Patients with Rhegmatogenous Retinal Detachment and Their Correlations with Clinical Features.
Takahashi S, Adachi K, Suzuki Y, Maeno A, Nakazawa M
BioMed research international20164256183(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

AG genotype of CCL2-2518 in combination with TT genotype of CFH Y402H showed significant protective effect against age-related macular degeneration.
Title: Evaluation of CC-cytokine ligand 2 and complementary factor H Y402H polymorphisms and their interactional association with age-related macular degeneration.

HUMSCs expressing ISZ-sTRAIL might be an efficient therapeutic approach against lung cancer and MCP-1/CCR2 axis is essential for the tumor tropism of HUMSCs
Title: Human umbilical cord mesenchymal stem cells delivering sTRAIL home to lung cancer mediated by MCP-1/CCR2 axis and exhibit antitumor effects.

Chemokine Ligand 2 (MCP-1/CCL2) and its receptor CCR2 are coexpressed in MePR-2B cells and are regulated upon differentiation, suggesting the presence of an autocrine and paracrine loop in differentiating cells. Moreover, we demonstrated that exogenous CCL2 is capable to affect neuro-glial differentiation in MePR-2B cells, thus providing novel evidences for the potential involvement of chemokine-mediated signaling in prog
Title: Secretome profiling of cytokines and growth factors reveals that neuro-glial differentiation is associated with the down-regulation of Chemokine Ligand 2 (MCP-1/CCL2) in amniotic fluid derived-mesenchymal progenitor cells.

Results indicate that urinary MCP-1 was associated with blood pressure elevation in this population of obese young adults.
Title: Urinary angiotensinogen excretion is associated with blood pressure in obese young adults.

MCP1 may have a role in coronary artery calcium progression in patients with HIV infection on stable antiretroviral therapy
Title: Non-Classical Monocytes and Monocyte Chemoattractant Protein-1 (MCP-1) Correlate with Coronary Artery Calcium Progression in Chronically HIV-1 Infected Adults on Stable Antiretroviral Therapy.

The following CCL2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CCL2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu18411 NM_002982.3
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Homo sapiens C-C motif chemokine ligand 2 (CCL2), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu18411
Clone ID Related Accession (Same CDS sequence) NM_002982.3
Accession Version NM_002982.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 300bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-30
Organism Homo sapiens(Human)
Product C-C motif chemokine 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG530064.1 and BC009716.1. This sequence is a reference standard in the RefSeqGene project. On Nov 29, 2004 this sequence version replaced NM_002982.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CA449867.1, CD522574.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
ATGAAAGTCT CTGCCGCCCT TCTGTGCCTG CTGCTCATAG CAGCCACCTT CATTCCCCAA 
GGGCTCGCTC AGCCAGATGC AATCAATGCC CCAGTCACCT GCTGTTATAA CTTCACCAAT
AGGAAGATCT CAGTGCAGAG GCTCGCGAGC TATAGAAGAA TCACCAGCAG CAAGTGTCCC
AAAGAAGCTG TGATCTTCAA GACCATTGTG GCCAAGGAGA TCTGTGCTGA CCCCAAGCAG
AAGTGGGTTC AGGATTCCAT GGACCACCTG GACAAGCAAA CCCAAACTCC GAAGACTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002973.1
CDS74..373
Misc Feature(1)143..145
Misc Feature(2)152..154
Misc Feature(3)152..154
Misc Feature(4)152..154
Misc Feature(5)164..166
Misc Feature(6)173..346
Misc Feature(7)order(173..187,191..214,245..250,314..346)
Misc Feature(8)order(179..181,185..187,224..226,230..232,248..250,263..271,293..301,317..322,329..334,341..346)
Misc Feature(9)order(179..181,185..187,239..241,245..250,266..268,290..292,296..298)
Misc Feature(10)order(179..181,266..268,293..295)
Misc Feature(11)order(179..187,191..214)
Misc Feature(12)179..181
Misc Feature(13)order(185..187,224..226,230..232,263..265,269..271,293..295,299..301,317..322,329..334,341..346)
Misc Feature(14)order(191..199,206..214,281..283,287..289)
Misc Feature(15)194..196
Misc Feature(16)197..199
Misc Feature(17)212..214
Misc Feature(18)order(230..232,254..256,308..310,314..316,323..328,335..337)
Misc Feature(19)245..250
Misc Feature(20)245..247
Misc Feature(21)254..256
Misc Feature(22)order(275..277,281..283)
Misc Feature(23)287..289
Misc Feature(24)314..316
Misc Feature(25)338..340
Exon (1)1..149
Exon (2)150..267
Exon (3)268..749
Translation

Target ORF information:

RefSeq Version NM_002982.3
Organism Homo sapiens(Human)
Definition Homo sapiens C-C motif chemokine ligand 2 (CCL2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002982.3

ORF Insert Sequence:

1
61
121
181
241
ATGAAAGTCT CTGCCGCCCT TCTGTGCCTG CTGCTCATAG CAGCCACCTT CATTCCCCAA 
GGGCTCGCTC AGCCAGATGC AATCAATGCC CCAGTCACCT GCTGTTATAA CTTCACCAAT
AGGAAGATCT CAGTGCAGAG GCTCGCGAGC TATAGAAGAA TCACCAGCAG CAAGTGTCCC
AAAGAAGCTG TGATCTTCAA GACCATTGTG GCCAAGGAGA TCTGTGCTGA CCCCAAGCAG
AAGTGGGTTC AGGATTCCAT GGACCACCTG GACAAGCAAA CCCAAACTCC GAAGACTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

CCL2 expression correlates with Snail expression and affects the prognosis of patients with gastric cancer.
Pathology, research and practice213(3)217-221(2017 Mar)
Zhang J,Yan Y,Cui X,Zhang J,Yang Y,Li H,Wu H,Li J,Wang L,Li M,Liu X,Wang J,Duan X


book

Specific tumor-derived CCL2 mediated by pyruvate kinase M2 in colorectal cancer cells contributes to macrophage recruitment in tumor microenvironment.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine39(3)1010428317695962(2017 Mar)
Zou K,Wang Y,Hu Y,Zheng L,Xu W,Li G


book

Plasma Monocyte Chemoattractant Protein 1 as a Predictive Marker for Sepsis Prognosis: A Prospective Cohort Study.
The Tohoku journal of experimental medicine241(2)139-147(2017 02)
Zhu T,Liao X,Feng T,Wu Q,Zhang J,Cao X,Li H


book

Urine Epidermal Growth Factor, Monocyte Chemoattractant Protein-1 or Their Ratio as Biomarkers for Interstitial Fibrosis and Tubular Atrophy in Primary Glomerulonephritis.
Kidney & blood pressure research41(6)997-1007(2016)
Worawichawong S,Worawichawong S,Radinahamed P,Muntham D,Sathirapongsasuti N,Nongnuch A,Assanatham M,Kitiyakara C


book

Profiles of Inflammatory Cytokines in the Vitreous Fluid from Patients with Rhegmatogenous Retinal Detachment and Their Correlations with Clinical Features.
BioMed research international20164256183(2016)
Takahashi S,Adachi K,Suzuki Y,Maeno A,Nakazawa M


 
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