AACS cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Gene Symbol	AACS
Entrez Gene ID	65985
Full Name	acetoacetyl-CoA synthetase
Synonyms	ACSF1,SUR-5
General protein information	Preferred Names acetoacetyl-CoA synthetase Names acetoacetyl-CoA synthetase acetoacetate-CoA ligase acyl-CoA synthetase family member 1 homolog of C. elegans supressor of ras 5 (sur-5) protein sur-5 homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q24.31
Disorder MIM:	614364

mRNA and Protein(s)

mRNA	Protein	Name
NM_001319840.1	NP_001306769.1	acetoacetyl-CoA synthetase isoform 3
NM_001319839.1	NP_001306768.1	acetoacetyl-CoA synthetase isoform 2
NM_023928.3	NP_076417.2	acetoacetyl-CoA synthetase
XM_011538692.2	XP_011536994.1	acetoacetyl-CoA synthetase isoform X1
NM_001319839.2	NP_001306768.1	acetoacetyl-CoA synthetase isoform 2
NM_023928.4	NP_076417.2	acetoacetyl-CoA synthetase isoform 1
NM_023928.5	NP_076417.2	acetoacetyl-CoA synthetase isoform 1
NM_001319840.2	NP_001306769.1	acetoacetyl-CoA synthetase isoform 3

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Aacs	NP_084486.1
Neurospora crassa	NCU00446	XP_956765.1
Danio rerio (zebrafish)	aacs	NP_957303.1
Caenorhabditis elegans (roundworm)	sur-5	NP_509229.1
Magnaporthe oryzae (rice blast fungus)	MGG_05196	XP_003712739.1
Canis lupus familiaris (dog)	AACS	XP_543365.3
Xenopus tropicalis (tropical clawed frog)	aacs	NP_001039244.1
Homo sapiens (human)	AACS	NP_076417.2
	AACS	XP_001103579.1
Rattus norvegicus (Norway rat)	Aacs	NP_075592.1
Pan troglodytes (chimpanzee)	AACS	XP_001135792.2
Bos taurus (cattle)	AACS	NP_001157401.1
Gallus gallus (chicken)	AACS	NP_001006184.1

Gene Ontology
Bibliography

Related articles in PubMed

Transcriptional regulation of the human acetoacetyl-CoA synthetase gene by PPARgamma.
Aguil? F, Camarero N, Relat J, Marrero PF, Haro D
The Biochemical journal427(2)255-64(2010 Mar)

Evidence for 26 distinct acyl-coenzyme A synthetase genes in the human genome.
Watkins PA, Maiguel D, Jia Z, Pevsner J
Journal of lipid research48(12)2736-50(2007 Dec)

Expression of acetoacetyl-CoA synthetase, a novel cytosolic ketone body-utilizing enzyme, in human brain.
Ohgami M, Takahashi N, Yamasaki M, Fukui T
Biochemical pharmacology65(6)989-94(2003 Mar)

Caenorhabditis elegans SUR-5, a novel but conserved protein, negatively regulates LET-60 Ras activity during vulval induction.
Gu T, Orita S, Han M
Molecular and cellular biology18(8)4556-64(1998 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the human AACS promoter is a PPARgamma target gene and this nuclear receptor is recruited to the AACS promoter by direct interaction with Sp1
Title: Transcriptional regulation of the human acetoacetyl-CoA synthetase gene by PPARgamma.

Observational study of gene-disease association. (HuGE Navigator)
Title: Multiple genetic variants along candidate pathways influence plasma high-density lipoprotein cholesterol concentrations.

The following AACS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AACS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu30063	NM_023928.5 Latest version!	Homo sapiens acetoacetyl-CoA synthetase (AACS), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu46803	NM_001319840.2 Latest version!	Homo sapiens acetoacetyl-CoA synthetase (AACS), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu46804	NM_001319839.2 Latest version!	Homo sapiens acetoacetyl-CoA synthetase (AACS), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu71674	XM_011538692.2 Latest version!	Homo sapiens acetoacetyl-CoA synthetase (AACS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
View Old Accession Versions >>
OHu46804	NM_001319839.1	Homo sapiens acetoacetyl-CoA synthetase (AACS), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu30063	NM_023928.3	Homo sapiens acetoacetyl-CoA synthetase (AACS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu30063	NM_023928.4	Homo sapiens acetoacetyl-CoA synthetase (AACS), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu46803	NM_001319840.1	Homo sapiens acetoacetyl-CoA synthetase (AACS), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AACS ( NM_023928.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_076417.2 Homo sapiens acetoacetyl-CoA synthetase (AACS), mRNA.

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CloneID	OHu30063
Clone ID Related Accession (Same CDS sequence)	NM_023928.3 , NM_023928.4 , NM_023928.5
Accession Version	NM_023928.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	acetoacetyl-CoA synthetase
Comment

ATGTCCAAGG AGGAGCGCCC CGGTCGGGAG GAGATCCTGG AGTGCCAGGT GATGTGGGAG 
CCTGACAGTA AGAAGAACAC GCAGATGGAC CGCTTCCGGG CGGCTGTGGG CGCCGCCTGC 
GGCCTGGCGC TGGAGAGTTA TGATGACTTG TACCATTGGT CCGTTGAGTC ATATTCAGAC 
TTCTGGGCAG AGTTCTGGAA ATTCAGTGGA ATTGTCTTCT CACGTGTGTA TGATGAGGTT 
GTGGACACAT CGAAAGGAAT CGCAGATGTC CCCGAGTGGT TCAAAGGCAG TCGGCTCAAC 
TATGCAGAAA ACCTCCTGCG GCACAAAGAG AATGACAGAG TTGCCCTTTA CATTGCAAGG 
GAAGGCAAAG AGGAAATTGT GAAGGTGACT TTTGAAGAGC TGAGGCAAGA AGTGGCTTTG 
TTTGCAGCAG CAATGAGGAA AATGGGTGTG AAGAAAGGAG ATCGGGTTGT TGGTTATTTA 
CCCAACAGTG AGCACGCTGT CGAGGCGATG CTGGCTGCGG CAAGCATTGG TGCCATCTGG 
AGCTCCACGT CCCCGGACTT CGGTGTGAAT GGTGTGCTGG ACCGGTTTTC TCAAATTCAG 
CCAAAGCTCA TCTTCTCTGT GGAGGCTGTT GTCTATAATG GCAAAGAGCA CAACCACATG 
GAAAAGCTGC AGCAGGTGGT TAAAGGCCTA CCAGACTTGA AGAAAGTGGT GGTGATTCCT 
TATGTGTCCT CCAGAGAGAA CATAGACCTT TCAAAGATTC CAAACAGTGT GTTTCTGGAT 
GACTTTCTTG CCACCGGCAC CAGTGAGCAG GCCCCGCAGC TGGAGTTCGA GCAGCTGCCC 
TTCAGCCACC CACTGTTCAT CATGTTCTCA TCGGGCACCA CGGGCGCACC CAAGTGCATG 
GTGCATTCCG CTGGGGGCAC CCTCATCCAG CATCTGAAGG AGCACCTGCT GCACGGCAAC 
ATGACCAGCA GTGACATCCT CCTGTGCTAC ACCACGGTCG GCTGGATGAT GTGGAACTGG 
ATGGTGTCCC TTCTGGCCAC AGGAGCGGCC ATGGTCTTGT ACGATGGCTC CCCCCTGGTG 
CCCACGCCCA ATGTGCTCTG GGACCTGGTT GACAGGATAG GCATCACTGT CCTGGTAACT 
GGGGCCAAGT GGCTGTCAGT GCTGGAAGAG AAGGCCATGA AGCCGGTGGA AACCCACAGT 
CTCCAGATGC TCCACACGAT CCTGTCCACT GGCTCCCCAC TGAAAGCCCA GAGCTACGAG 
TATGTCTACA GGTGCATCAA GAGCAGCATC CTCCTGGGCT CCATCTCAGG AGGCACCGAC 
ATCATCTCCT GCTTCATGGG CCACAATTTT TCTCTTCCTG TGTATAAAGG GGAGATTCAG 
GCCCGGAACC TGGGCATGGC CGTGGAAGCG TGGAACGAGG AAGGAAAGGC GGTCTGGGGA 
GAGAGCGGCG AGCTGGTGTG TACTAAGCCG ATCCCTTGCC AGCCCACACA CTTCTGGAAC 
GATGAGAACG GCAACAAGTA CAGGAAGGCG TATTTCTCCA AATTCCCAGG TATCTGGGCT 
CATGGCGACT ACTGCAGAAT CAACCCCAAG ACCGGGGGCA TCGTCATGCT TGGCCGGAGT 
GACGGCACCC TCAACCCCAA CGGGGTGCGG TTCGGCAGCT CGGAAATCTA TAACATTGTG 
GAATCCTTCG AGGAGGTGGA GGACAGCCTG TGTGTCCCCC AGTATAACAA GTACAGGGAG 
GAGAGGGTGA TCCTCTTCCT GAAGATGGCC TCCGGGCACG CCTTCCAGCC TGACTTGGTT 
AAGAGGATCC GTGACGCCAT CCGCATGGGC TTGTCTGCGC GACACGTGCC CAGCCTCATC 
CTGGAAACCA AGGGCATCCC GTATACGCTC AACGGCAAGA AAGTGGAAGT TGCCGTCAAA 
CAGATCATCG CTGGAAAAGC CGTGGAGCAA GGAGGTGCTT TCTCGAACCC CGAGACCCTG 
GATCTGTACC GGGACATCCC TGAGCTGCAG GGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_076417.2

Translation

MSKEERPGREEILECQVMWEPDSKKNTQMDRFRAAVGAACGLALESYDDLYHWSVESYSDFWAEFWKFSGIVFSRVYDEVVDTSKGIADVPEWFKGSRLNYAENLLRHKENDRVALYIAREGKEEIVKVTFEELRQEVALFAAAMRKMGVKKGDRVVGYLPNSEHAVEAMLAAASIGAIWSSTSPDFGVNGVLDRFSQIQPKLIFSVEAVVYNGKEHNHMEKLQQVVKGLPDLKKVVVIPYVSSRENIDLSKIPNSVFLDDFLATGTSEQAPQLEFEQLPFSHPLFIMFSSGTTGAPKCMVHSAGGTLIQHLKEHLLHGNMTSSDILLCYTTVGWMMWNWMVSLLATGAAMVLYDGSPLVPTPNVLWDLVDRIGITVLVTGAKWLSVLEEKAMKPVETHSLQMLHTILSTGSPLKAQSYEYVYRCIKSSILLGSISGGTDIISCFMGHNFSLPVYKGEIQARNLGMAVEAWNEEGKAVWGESGELVCTKPIPCQPTHFWNDENGNKYRKAYFSKFPGIWAHGDYCRINPKTGGIVMLGRSDGTLNPNGVRFGSSEIYNIVESFEEVEDSLCVPQYNKYREERVILFLKMASGHAFQPDLVKRIRDAIRMGLSARHVPSLILETKGIPYTLNGKKVEVAVKQIIAGKAVEQGGAFSNPETLDLYRDIPELQGF

Target ORF information:

RefSeq Version	NM_023928.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens acetoacetyl-CoA synthetase (AACS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023928.3

ORF Insert Sequence:

ATGTCCAAGG AGGAGCGCCC CGGTCGGGAG GAGATCCTGG AGTGCCAGGT GATGTGGGAG 
CCTGACAGTA AGAAGAACAC GCAGATGGAC CGCTTCCGGG CGGCTGTGGG CGCCGCCTGC 
GGCCTGGCGC TGGAGAGTTA TGATGACTTG TACCATTGGT CCGTTGAGTC ATATTCAGAC 
TTCTGGGCAG AGTTCTGGAA ATTCAGTGGA ATTGTCTTCT CACGTGTGTA TGATGAGGTT 
GTGGACACAT CGAAAGGAAT CGCAGATGTC CCCGAGTGGT TCAAAGGCAG TCGGCTCAAC 
TATGCAGAAA ACCTCCTGCG GCACAAAGAG AATGACAGAG TTGCCCTTTA CATTGCAAGG 
GAAGGCAAAG AGGAAATTGT GAAGGTGACT TTTGAAGAGC TGAGGCAAGA AGTGGCTTTG 
TTTGCAGCAG CAATGAGGAA AATGGGTGTG AAGAAAGGAG ATCGGGTTGT TGGTTATTTA 
CCCAACAGTG AGCACGCTGT CGAGGCGATG CTGGCTGCGG CAAGCATTGG TGCCATCTGG 
AGCTCCACGT CCCCGGACTT CGGTGTGAAT GGTGTGCTGG ACCGGTTTTC TCAAATTCAG 
CCAAAGCTCA TCTTCTCTGT GGAGGCTGTT GTCTATAATG GCAAAGAGCA CAACCACATG 
GAAAAGCTGC AGCAGGTGGT TAAAGGCCTA CCAGACTTGA AGAAAGTGGT GGTGATTCCT 
TATGTGTCCT CCAGAGAGAA CATAGACCTT TCAAAGATTC CAAACAGTGT GTTTCTGGAT 
GACTTTCTTG CCACCGGCAC CAGTGAGCAG GCCCCGCAGC TGGAGTTCGA GCAGCTGCCC 
TTCAGCCACC CACTGTTCAT CATGTTCTCA TCGGGCACCA CGGGCGCACC CAAGTGCATG 
GTGCATTCCG CTGGGGGCAC CCTCATCCAG CATCTGAAGG AGCACCTGCT GCACGGCAAC 
ATGACCAGCA GTGACATCCT CCTGTGCTAC ACCACGGTCG GCTGGATGAT GTGGAACTGG 
ATGGTGTCCC TTCTGGCCAC AGGAGCGGCC ATGGTCTTGT ACGATGGCTC CCCCCTGGTG 
CCCACGCCCA ATGTGCTCTG GGACCTGGTT GACAGGATAG GCATCACTGT CCTGGTAACT 
GGGGCCAAGT GGCTGTCAGT GCTGGAAGAG AAGGCCATGA AGCCGGTGGA AACCCACAGT 
CTCCAGATGC TCCACACGAT CCTGTCCACT GGCTCCCCAC TGAAAGCCCA GAGCTACGAG 
TATGTCTACA GGTGCATCAA GAGCAGCATC CTCCTGGGCT CCATCTCAGG AGGCACCGAC 
ATCATCTCCT GCTTCATGGG CCACAATTTT TCTCTTCCTG TGTATAAAGG GGAGATTCAG 
GCCCGGAACC TGGGCATGGC CGTGGAAGCG TGGAACGAGG AAGGAAAGGC GGTCTGGGGA 
GAGAGCGGCG AGCTGGTGTG TACTAAGCCG ATCCCTTGCC AGCCCACACA CTTCTGGAAC 
GATGAGAACG GCAACAAGTA CAGGAAGGCG TATTTCTCCA AATTCCCAGG TATCTGGGCT 
CATGGCGACT ACTGCAGAAT CAACCCCAAG ACCGGGGGCA TCGTCATGCT TGGCCGGAGT 
GACGGCACCC TCAACCCCAA CGGGGTGCGG TTCGGCAGCT CGGAAATCTA TAACATTGTG 
GAATCCTTCG AGGAGGTGGA GGACAGCCTG TGTGTCCCCC AGTATAACAA GTACAGGGAG 
GAGAGGGTGA TCCTCTTCCT GAAGATGGCC TCCGGGCACG CCTTCCAGCC TGACTTGGTT 
AAGAGGATCC GTGACGCCAT CCGCATGGGC TTGTCTGCGC GACACGTGCC CAGCCTCATC 
CTGGAAACCA AGGGCATCCC GTATACGCTC AACGGCAAGA AAGTGGAAGT TGCCGTCAAA 
CAGATCATCG CTGGAAAAGC CGTGGAGCAA GGAGGTGCTT TCTCGAACCC CGAGACCCTG 
GATCTGTACC GGGACATCCC TGAGCTGCAG GGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AACS ( NM_023928.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_076417.2 Homo sapiens acetoacetyl-CoA synthetase (AACS), transcript variant 1, mRNA.

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CloneID	OHu30063
Clone ID Related Accession (Same CDS sequence)	NM_023928.3 , NM_023928.4 , NM_023928.5
Accession Version	NM_023928.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	acetoacetyl-CoA synthetase isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY018418.1, BC040490.1, BC051862.1 and BM975973.1. On Feb 5, 2016 this sequence version replaced NM_023928.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC040490.1, SRR1803614.224335.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAAGG AGGAGCGCCC CGGTCGGGAG GAGATCCTGG AGTGCCAGGT GATGTGGGAG 
CCTGACAGTA AGAAGAACAC GCAGATGGAC CGCTTCCGGG CGGCTGTGGG CGCCGCCTGC 
GGCCTGGCGC TGGAGAGTTA TGATGACTTG TACCATTGGT CCGTTGAGTC ATATTCAGAC 
TTCTGGGCAG AGTTCTGGAA ATTCAGTGGA ATTGTCTTCT CACGTGTGTA TGATGAGGTT 
GTGGACACAT CGAAAGGAAT CGCAGATGTC CCCGAGTGGT TCAAAGGCAG TCGGCTCAAC 
TATGCAGAAA ACCTCCTGCG GCACAAAGAG AATGACAGAG TTGCCCTTTA CATTGCAAGG 
GAAGGCAAAG AGGAAATTGT GAAGGTGACT TTTGAAGAGC TGAGGCAAGA AGTGGCTTTG 
TTTGCAGCAG CAATGAGGAA AATGGGTGTG AAGAAAGGAG ATCGGGTTGT TGGTTATTTA 
CCCAACAGTG AGCACGCTGT CGAGGCGATG CTGGCTGCGG CAAGCATTGG TGCCATCTGG 
AGCTCCACGT CCCCGGACTT CGGTGTGAAT GGTGTGCTGG ACCGGTTTTC TCAAATTCAG 
CCAAAGCTCA TCTTCTCTGT GGAGGCTGTT GTCTATAATG GCAAAGAGCA CAACCACATG 
GAAAAGCTGC AGCAGGTGGT TAAAGGCCTA CCAGACTTGA AGAAAGTGGT GGTGATTCCT 
TATGTGTCCT CCAGAGAGAA CATAGACCTT TCAAAGATTC CAAACAGTGT GTTTCTGGAT 
GACTTTCTTG CCACCGGCAC CAGTGAGCAG GCCCCGCAGC TGGAGTTCGA GCAGCTGCCC 
TTCAGCCACC CACTGTTCAT CATGTTCTCA TCGGGCACCA CGGGCGCACC CAAGTGCATG 
GTGCATTCCG CTGGGGGCAC CCTCATCCAG CATCTGAAGG AGCACCTGCT GCACGGCAAC 
ATGACCAGCA GTGACATCCT CCTGTGCTAC ACCACGGTCG GCTGGATGAT GTGGAACTGG 
ATGGTGTCCC TTCTGGCCAC AGGAGCGGCC ATGGTCTTGT ACGATGGCTC CCCCCTGGTG 
CCCACGCCCA ATGTGCTCTG GGACCTGGTT GACAGGATAG GCATCACTGT CCTGGTAACT 
GGGGCCAAGT GGCTGTCAGT GCTGGAAGAG AAGGCCATGA AGCCGGTGGA AACCCACAGT 
CTCCAGATGC TCCACACGAT CCTGTCCACT GGCTCCCCAC TGAAAGCCCA GAGCTACGAG 
TATGTCTACA GGTGCATCAA GAGCAGCATC CTCCTGGGCT CCATCTCAGG AGGCACCGAC 
ATCATCTCCT GCTTCATGGG CCACAATTTT TCTCTTCCTG TGTATAAAGG GGAGATTCAG 
GCCCGGAACC TGGGCATGGC CGTGGAAGCG TGGAACGAGG AAGGAAAGGC GGTCTGGGGA 
GAGAGCGGCG AGCTGGTGTG TACTAAGCCG ATCCCTTGCC AGCCCACACA CTTCTGGAAC 
GATGAGAACG GCAACAAGTA CAGGAAGGCG TATTTCTCCA AATTCCCAGG TATCTGGGCT 
CATGGCGACT ACTGCAGAAT CAACCCCAAG ACCGGGGGCA TCGTCATGCT TGGCCGGAGT 
GACGGCACCC TCAACCCCAA CGGGGTGCGG TTCGGCAGCT CGGAAATCTA TAACATTGTG 
GAATCCTTCG AGGAGGTGGA GGACAGCCTG TGTGTCCCCC AGTATAACAA GTACAGGGAG 
GAGAGGGTGA TCCTCTTCCT GAAGATGGCC TCCGGGCACG CCTTCCAGCC TGACTTGGTT 
AAGAGGATCC GTGACGCCAT CCGCATGGGC TTGTCTGCGC GACACGTGCC CAGCCTCATC 
CTGGAAACCA AGGGCATCCC GTATACGCTC AACGGCAAGA AAGTGGAAGT TGCCGTCAAA 
CAGATCATCG CTGGAAAAGC CGTGGAGCAA GGAGGTGCTT TCTCGAACCC CGAGACCCTG 
GATCTGTACC GGGACATCCC TGAGCTGCAG GGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_076417.2
CDS	219..2237
Translation	MSKEERPGREEILECQVMWEPDSKKNTQMDRFRAAVGAACGLALESYDDLYHWSVESYSDFWAEFWKFSGIVFSRVYDEVVDTSKGIADVPEWFKGSRLNYAENLLRHKENDRVALYIAREGKEEIVKVTFEELRQEVALFAAAMRKMGVKKGDRVVGYLPNSEHAVEAMLAAASIGAIWSSTSPDFGVNGVLDRFSQIQPKLIFSVEAVVYNGKEHNHMEKLQQVVKGLPDLKKVVVIPYVSSRENIDLSKIPNSVFLDDFLATGTSEQAPQLEFEQLPFSHPLFIMFSSGTTGAPKCMVHSAGGTLIQHLKEHLLHGNMTSSDILLCYTTVGWMMWNWMVSLLATGAAMVLYDGSPLVPTPNVLWDLVDRIGITVLVTGAKWLSVLEEKAMKPVETHSLQMLHTILSTGSPLKAQSYEYVYRCIKSSILLGSISGGTDIISCFMGHNFSLPVYKGEIQARNLGMAVEAWNEEGKAVWGESGELVCTKPIPCQPTHFWNDENGNKYRKAYFSKFPGIWAHGDYCRINPKTGGIVMLGRSDGTLNPNGVRFGSSEIYNIVESFEEVEDSLCVPQYNKYREERVILFLKMASGHAFQPDLVKRIRDAIRMGLSARHVPSLILETKGIPYTLNGKKVEVAVKQIIAGKAVEQGGAFSNPETLDLYRDIPELQGF

Target ORF information:

RefSeq Version	NM_023928.4
Organism	Homo sapiens(human)
Definition	Homo sapiens acetoacetyl-CoA synthetase (AACS), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023928.4

ORF Insert Sequence:

ATGTCCAAGG AGGAGCGCCC CGGTCGGGAG GAGATCCTGG AGTGCCAGGT GATGTGGGAG 
CCTGACAGTA AGAAGAACAC GCAGATGGAC CGCTTCCGGG CGGCTGTGGG CGCCGCCTGC 
GGCCTGGCGC TGGAGAGTTA TGATGACTTG TACCATTGGT CCGTTGAGTC ATATTCAGAC 
TTCTGGGCAG AGTTCTGGAA ATTCAGTGGA ATTGTCTTCT CACGTGTGTA TGATGAGGTT 
GTGGACACAT CGAAAGGAAT CGCAGATGTC CCCGAGTGGT TCAAAGGCAG TCGGCTCAAC 
TATGCAGAAA ACCTCCTGCG GCACAAAGAG AATGACAGAG TTGCCCTTTA CATTGCAAGG 
GAAGGCAAAG AGGAAATTGT GAAGGTGACT TTTGAAGAGC TGAGGCAAGA AGTGGCTTTG 
TTTGCAGCAG CAATGAGGAA AATGGGTGTG AAGAAAGGAG ATCGGGTTGT TGGTTATTTA 
CCCAACAGTG AGCACGCTGT CGAGGCGATG CTGGCTGCGG CAAGCATTGG TGCCATCTGG 
AGCTCCACGT CCCCGGACTT CGGTGTGAAT GGTGTGCTGG ACCGGTTTTC TCAAATTCAG 
CCAAAGCTCA TCTTCTCTGT GGAGGCTGTT GTCTATAATG GCAAAGAGCA CAACCACATG 
GAAAAGCTGC AGCAGGTGGT TAAAGGCCTA CCAGACTTGA AGAAAGTGGT GGTGATTCCT 
TATGTGTCCT CCAGAGAGAA CATAGACCTT TCAAAGATTC CAAACAGTGT GTTTCTGGAT 
GACTTTCTTG CCACCGGCAC CAGTGAGCAG GCCCCGCAGC TGGAGTTCGA GCAGCTGCCC 
TTCAGCCACC CACTGTTCAT CATGTTCTCA TCGGGCACCA CGGGCGCACC CAAGTGCATG 
GTGCATTCCG CTGGGGGCAC CCTCATCCAG CATCTGAAGG AGCACCTGCT GCACGGCAAC 
ATGACCAGCA GTGACATCCT CCTGTGCTAC ACCACGGTCG GCTGGATGAT GTGGAACTGG 
ATGGTGTCCC TTCTGGCCAC AGGAGCGGCC ATGGTCTTGT ACGATGGCTC CCCCCTGGTG 
CCCACGCCCA ATGTGCTCTG GGACCTGGTT GACAGGATAG GCATCACTGT CCTGGTAACT 
GGGGCCAAGT GGCTGTCAGT GCTGGAAGAG AAGGCCATGA AGCCGGTGGA AACCCACAGT 
CTCCAGATGC TCCACACGAT CCTGTCCACT GGCTCCCCAC TGAAAGCCCA GAGCTACGAG 
TATGTCTACA GGTGCATCAA GAGCAGCATC CTCCTGGGCT CCATCTCAGG AGGCACCGAC 
ATCATCTCCT GCTTCATGGG CCACAATTTT TCTCTTCCTG TGTATAAAGG GGAGATTCAG 
GCCCGGAACC TGGGCATGGC CGTGGAAGCG TGGAACGAGG AAGGAAAGGC GGTCTGGGGA 
GAGAGCGGCG AGCTGGTGTG TACTAAGCCG ATCCCTTGCC AGCCCACACA CTTCTGGAAC 
GATGAGAACG GCAACAAGTA CAGGAAGGCG TATTTCTCCA AATTCCCAGG TATCTGGGCT 
CATGGCGACT ACTGCAGAAT CAACCCCAAG ACCGGGGGCA TCGTCATGCT TGGCCGGAGT 
GACGGCACCC TCAACCCCAA CGGGGTGCGG TTCGGCAGCT CGGAAATCTA TAACATTGTG 
GAATCCTTCG AGGAGGTGGA GGACAGCCTG TGTGTCCCCC AGTATAACAA GTACAGGGAG 
GAGAGGGTGA TCCTCTTCCT GAAGATGGCC TCCGGGCACG CCTTCCAGCC TGACTTGGTT 
AAGAGGATCC GTGACGCCAT CCGCATGGGC TTGTCTGCGC GACACGTGCC CAGCCTCATC 
CTGGAAACCA AGGGCATCCC GTATACGCTC AACGGCAAGA AAGTGGAAGT TGCCGTCAAA 
CAGATCATCG CTGGAAAAGC CGTGGAGCAA GGAGGTGCTT TCTCGAACCC CGAGACCCTG 
GATCTGTACC GGGACATCCC TGAGCTGCAG GGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AACS ( NM_023928.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_076417.2 Homo sapiens acetoacetyl-CoA synthetase (AACS), transcript variant 1, mRNA.

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CloneID	OHu30063
Clone ID Related Accession (Same CDS sequence)	NM_023928.3 , NM_023928.4 , NM_023928.5
Accession Version	NM_023928.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-22
Organism	Homo sapiens(human)
Product	acetoacetyl-CoA synthetase isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC040490.1 and BC051862.1. On Nov 23, 2018 this sequence version replaced NM_023928.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC040490.1, SRR1803614.224335.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000316519.11/ ENSP00000324842.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCCAAGG AGGAGCGCCC CGGTCGGGAG GAGATCCTGG AGTGCCAGGT GATGTGGGAG 
CCTGACAGTA AGAAGAACAC GCAGATGGAC CGCTTCCGGG CGGCTGTGGG CGCCGCCTGC 
GGCCTGGCGC TGGAGAGTTA TGATGACTTG TACCATTGGT CCGTTGAGTC ATATTCAGAC 
TTCTGGGCAG AGTTCTGGAA ATTCAGTGGA ATTGTCTTCT CACGTGTGTA TGATGAGGTT 
GTGGACACAT CGAAAGGAAT CGCAGATGTC CCCGAGTGGT TCAAAGGCAG TCGGCTCAAC 
TATGCAGAAA ACCTCCTGCG GCACAAAGAG AATGACAGAG TTGCCCTTTA CATTGCAAGG 
GAAGGCAAAG AGGAAATTGT GAAGGTGACT TTTGAAGAGC TGAGGCAAGA AGTGGCTTTG 
TTTGCAGCAG CAATGAGGAA AATGGGTGTG AAGAAAGGAG ATCGGGTTGT TGGTTATTTA 
CCCAACAGTG AGCACGCTGT CGAGGCGATG CTGGCTGCGG CAAGCATTGG TGCCATCTGG 
AGCTCCACGT CCCCGGACTT CGGTGTGAAT GGTGTGCTGG ACCGGTTTTC TCAAATTCAG 
CCAAAGCTCA TCTTCTCTGT GGAGGCTGTT GTCTATAATG GCAAAGAGCA CAACCACATG 
GAAAAGCTGC AGCAGGTGGT TAAAGGCCTA CCAGACTTGA AGAAAGTGGT GGTGATTCCT 
TATGTGTCCT CCAGAGAGAA CATAGACCTT TCAAAGATTC CAAACAGTGT GTTTCTGGAT 
GACTTTCTTG CCACCGGCAC CAGTGAGCAG GCCCCGCAGC TGGAGTTCGA GCAGCTGCCC 
TTCAGCCACC CACTGTTCAT CATGTTCTCA TCGGGCACCA CGGGCGCACC CAAGTGCATG 
GTGCATTCCG CTGGGGGCAC CCTCATCCAG CATCTGAAGG AGCACCTGCT GCACGGCAAC 
ATGACCAGCA GTGACATCCT CCTGTGCTAC ACCACGGTCG GCTGGATGAT GTGGAACTGG 
ATGGTGTCCC TTCTGGCCAC AGGAGCGGCC ATGGTCTTGT ACGATGGCTC CCCCCTGGTG 
CCCACGCCCA ATGTGCTCTG GGACCTGGTT GACAGGATAG GCATCACTGT CCTGGTAACT 
GGGGCCAAGT GGCTGTCAGT GCTGGAAGAG AAGGCCATGA AGCCGGTGGA AACCCACAGT 
CTCCAGATGC TCCACACGAT CCTGTCCACT GGCTCCCCAC TGAAAGCCCA GAGCTACGAG 
TATGTCTACA GGTGCATCAA GAGCAGCATC CTCCTGGGCT CCATCTCAGG AGGCACCGAC 
ATCATCTCCT GCTTCATGGG CCACAATTTT TCTCTTCCTG TGTATAAAGG GGAGATTCAG 
GCCCGGAACC TGGGCATGGC CGTGGAAGCG TGGAACGAGG AAGGAAAGGC GGTCTGGGGA 
GAGAGCGGCG AGCTGGTGTG TACTAAGCCG ATCCCTTGCC AGCCCACACA CTTCTGGAAC 
GATGAGAACG GCAACAAGTA CAGGAAGGCG TATTTCTCCA AATTCCCAGG TATCTGGGCT 
CATGGCGACT ACTGCAGAAT CAACCCCAAG ACCGGGGGCA TCGTCATGCT TGGCCGGAGT 
GACGGCACCC TCAACCCCAA CGGGGTGCGG TTCGGCAGCT CGGAAATCTA TAACATTGTG 
GAATCCTTCG AGGAGGTGGA GGACAGCCTG TGTGTCCCCC AGTATAACAA GTACAGGGAG 
GAGAGGGTGA TCCTCTTCCT GAAGATGGCC TCCGGGCACG CCTTCCAGCC TGACTTGGTT 
AAGAGGATCC GTGACGCCAT CCGCATGGGC TTGTCTGCGC GACACGTGCC CAGCCTCATC 
CTGGAAACCA AGGGCATCCC GTATACGCTC AACGGCAAGA AAGTGGAAGT TGCCGTCAAA 
CAGATCATCG CTGGAAAAGC CGTGGAGCAA GGAGGTGCTT TCTCGAACCC CGAGACCCTG 
GATCTGTACC GGGACATCCC TGAGCTGCAG GGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_076417.2
CDS	151..2169
Translation	MSKEERPGREEILECQVMWEPDSKKNTQMDRFRAAVGAACGLALESYDDLYHWSVESYSDFWAEFWKFSGIVFSRVYDEVVDTSKGIADVPEWFKGSRLNYAENLLRHKENDRVALYIAREGKEEIVKVTFEELRQEVALFAAAMRKMGVKKGDRVVGYLPNSEHAVEAMLAAASIGAIWSSTSPDFGVNGVLDRFSQIQPKLIFSVEAVVYNGKEHNHMEKLQQVVKGLPDLKKVVVIPYVSSRENIDLSKIPNSVFLDDFLATGTSEQAPQLEFEQLPFSHPLFIMFSSGTTGAPKCMVHSAGGTLIQHLKEHLLHGNMTSSDILLCYTTVGWMMWNWMVSLLATGAAMVLYDGSPLVPTPNVLWDLVDRIGITVLVTGAKWLSVLEEKAMKPVETHSLQMLHTILSTGSPLKAQSYEYVYRCIKSSILLGSISGGTDIISCFMGHNFSLPVYKGEIQARNLGMAVEAWNEEGKAVWGESGELVCTKPIPCQPTHFWNDENGNKYRKAYFSKFPGIWAHGDYCRINPKTGGIVMLGRSDGTLNPNGVRFGSSEIYNIVESFEEVEDSLCVPQYNKYREERVILFLKMASGHAFQPDLVKRIRDAIRMGLSARHVPSLILETKGIPYTLNGKKVEVAVKQIIAGKAVEQGGAFSNPETLDLYRDIPELQGF

Target ORF information:

RefSeq Version	NM_023928.5
Organism	Homo sapiens(human)
Definition	Homo sapiens acetoacetyl-CoA synthetase (AACS), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023928.5

ORF Insert Sequence:

ATGTCCAAGG AGGAGCGCCC CGGTCGGGAG GAGATCCTGG AGTGCCAGGT GATGTGGGAG 
CCTGACAGTA AGAAGAACAC GCAGATGGAC CGCTTCCGGG CGGCTGTGGG CGCCGCCTGC 
GGCCTGGCGC TGGAGAGTTA TGATGACTTG TACCATTGGT CCGTTGAGTC ATATTCAGAC 
TTCTGGGCAG AGTTCTGGAA ATTCAGTGGA ATTGTCTTCT CACGTGTGTA TGATGAGGTT 
GTGGACACAT CGAAAGGAAT CGCAGATGTC CCCGAGTGGT TCAAAGGCAG TCGGCTCAAC 
TATGCAGAAA ACCTCCTGCG GCACAAAGAG AATGACAGAG TTGCCCTTTA CATTGCAAGG 
GAAGGCAAAG AGGAAATTGT GAAGGTGACT TTTGAAGAGC TGAGGCAAGA AGTGGCTTTG 
TTTGCAGCAG CAATGAGGAA AATGGGTGTG AAGAAAGGAG ATCGGGTTGT TGGTTATTTA 
CCCAACAGTG AGCACGCTGT CGAGGCGATG CTGGCTGCGG CAAGCATTGG TGCCATCTGG 
AGCTCCACGT CCCCGGACTT CGGTGTGAAT GGTGTGCTGG ACCGGTTTTC TCAAATTCAG 
CCAAAGCTCA TCTTCTCTGT GGAGGCTGTT GTCTATAATG GCAAAGAGCA CAACCACATG 
GAAAAGCTGC AGCAGGTGGT TAAAGGCCTA CCAGACTTGA AGAAAGTGGT GGTGATTCCT 
TATGTGTCCT CCAGAGAGAA CATAGACCTT TCAAAGATTC CAAACAGTGT GTTTCTGGAT 
GACTTTCTTG CCACCGGCAC CAGTGAGCAG GCCCCGCAGC TGGAGTTCGA GCAGCTGCCC 
TTCAGCCACC CACTGTTCAT CATGTTCTCA TCGGGCACCA CGGGCGCACC CAAGTGCATG 
GTGCATTCCG CTGGGGGCAC CCTCATCCAG CATCTGAAGG AGCACCTGCT GCACGGCAAC 
ATGACCAGCA GTGACATCCT CCTGTGCTAC ACCACGGTCG GCTGGATGAT GTGGAACTGG 
ATGGTGTCCC TTCTGGCCAC AGGAGCGGCC ATGGTCTTGT ACGATGGCTC CCCCCTGGTG 
CCCACGCCCA ATGTGCTCTG GGACCTGGTT GACAGGATAG GCATCACTGT CCTGGTAACT 
GGGGCCAAGT GGCTGTCAGT GCTGGAAGAG AAGGCCATGA AGCCGGTGGA AACCCACAGT 
CTCCAGATGC TCCACACGAT CCTGTCCACT GGCTCCCCAC TGAAAGCCCA GAGCTACGAG 
TATGTCTACA GGTGCATCAA GAGCAGCATC CTCCTGGGCT CCATCTCAGG AGGCACCGAC 
ATCATCTCCT GCTTCATGGG CCACAATTTT TCTCTTCCTG TGTATAAAGG GGAGATTCAG 
GCCCGGAACC TGGGCATGGC CGTGGAAGCG TGGAACGAGG AAGGAAAGGC GGTCTGGGGA 
GAGAGCGGCG AGCTGGTGTG TACTAAGCCG ATCCCTTGCC AGCCCACACA CTTCTGGAAC 
GATGAGAACG GCAACAAGTA CAGGAAGGCG TATTTCTCCA AATTCCCAGG TATCTGGGCT 
CATGGCGACT ACTGCAGAAT CAACCCCAAG ACCGGGGGCA TCGTCATGCT TGGCCGGAGT 
GACGGCACCC TCAACCCCAA CGGGGTGCGG TTCGGCAGCT CGGAAATCTA TAACATTGTG 
GAATCCTTCG AGGAGGTGGA GGACAGCCTG TGTGTCCCCC AGTATAACAA GTACAGGGAG 
GAGAGGGTGA TCCTCTTCCT GAAGATGGCC TCCGGGCACG CCTTCCAGCC TGACTTGGTT 
AAGAGGATCC GTGACGCCAT CCGCATGGGC TTGTCTGCGC GACACGTGCC CAGCCTCATC 
CTGGAAACCA AGGGCATCCC GTATACGCTC AACGGCAAGA AAGTGGAAGT TGCCGTCAAA 
CAGATCATCG CTGGAAAAGC CGTGGAGCAA GGAGGTGCTT TCTCGAACCC CGAGACCCTG 
GATCTGTACC GGGACATCCC TGAGCTGCAG GGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AACS ( NM_001319840.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001306769.1 Homo sapiens acetoacetyl-CoA synthetase (AACS), transcript variant 3, mRNA.

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CloneID	OHu46803
Clone ID Related Accession (Same CDS sequence)	NM_001319840.1 , NM_001319840.2
Accession Version	NM_001319840.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	acetoacetyl-CoA synthetase isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC122688.5. On Feb 5, 2016 this sequence version replaced XM_005253609.2. Transcript Variant: This variant (3) lacks an alternate in-frame exon compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.171002.1, SRR1803616.234506.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAAGG AGGAGCGCCC CGGTCGGGAG GAGATCCTGG AGTGCCAGGT GATGTGGGAG 
CCTGACAGTA AGAAGAACAC GCAGATGGAC CGCTTCCGGG CGGCTGTGGG CGCCGCCTGC 
GGCCTGGCGC TGGAGAGTTA TGATGACTTG TACCATTGGT CCGTTGAGTC ATATTCAGAC 
TTCTGGGCAG AGTTCTGGAA ATTCAGTGGA ATTGTCTTCT CACGTGTGTA TGATGAGGTT 
GTGGACACAT CGAAAGGAAT CGCAGATGTC CCCGAGTGGT TCAAAGGCAG TCGGCTCAAC 
TATGCAGAAA ACCTCCTGCG GCACAAAGAG AATGACAGAG TTGCCCTTTA CATTGCAAGG 
GAAGGCAAAG AGGAAATTGT GAAGGTGACT TTTGAAGAGC TGAGGCAAGA AGTGGCTTTG 
TTTGCAGCAG CAATGAGGAA AATGGGTGTG AAGAAAGGAG ATCGGGTTGT TGGTTATTTA 
CCCAACAGTG AGCACGCTGT CGAGGCGATG CTGGCTGCGG CAAGCATTGG TGCCATCTGG 
AGCTCCACGT CCCCGGACTT CGGTGTGAAT GGTGTGCTGG ACCGGTTTTC TCAAATTCAG 
CCAAAGCTCA TCTTCTCTGT GGAGGCTGTT GTCTATAATG GCAAAGAGCA CAACCACATG 
GAAAAGCTGC AGCAGGTGGT TAAAGGCCTA CCAGACTTGA AGAAAGTGGT GGTGATTCCT 
TATGTGTCCT CCAGAGAGAA CATAGACCTT TCAAAGATTC CAAACAGTGT GTTTCTGGAT 
GACTTTCTTG CCACCGGCAC CAGTGAGCAG GCCCCGCAGC TGGAGTTCGA GCAGCTGCCC 
TTCAGCCACC CACTGTTCAT CATGTTCTCA TCGGGCACCA CGGGCGCACC CAAGTGCATG 
GTGCATTCCG CTGGGGGCAC CCTCATCCAG CATCTGAAGG AGCACCTGCT GCACGGCAAC 
ATGACCAGCA GTGACATCCT CCTGTGCTAC ACCACGGTCG GCTGGATGAT GTGGAACTGG 
ATGGTGTCCC TTCTGGCCAC AGGAGCGGCC ATGGTCTTGT ACGATGGCTC CCCCCTGGTG 
CCCACGCCCA ATGTGCTCTG GGACCTGGTT GACAGGATAG GCATCACTGT CCTGGTAACT 
GGGGCCAAGT GGCTGTCAGT GCTGGAAGAG AAGGCCATGA AGCCGGGAGG CACCGACATC 
ATCTCCTGCT TCATGGGCCA CAATTTTTCT CTTCCTGTGT ATAAAGGGGA GATTCAGGCC 
CGGAACCTGG GCATGGCCGT GGAAGCGTGG AACGAGGAAG GAAAGGCGGT CTGGGGAGAG 
AGCGGCGAGC TGGTGTGTAC TAAGCCGATC CCTTGCCAGC CCACACACTT CTGGAACGAT 
GAGAACGGCA ACAAGTACAG GAAGGCGTAT TTCTCCAAAT TCCCAGGTAT CTGGGCTCAT 
GGCGACTACT GCAGAATCAA CCCCAAGACC GGGGGCATCG TCATGCTTGG CCGGAGTGAC 
GGCACCCTCA ACCCCAACGG GGTGCGGTTC GGCAGCTCGG AAATCTATAA CATTGTGGAA 
TCCTTCGAGG AGGTGGAGGA CAGCCTGTGT GTCCCCCAGT ATAACAAGTA CAGGGAGGAG 
AGGGTGATCC TCTTCCTGAA GATGGCCTCC GGGCACGCCT TCCAGCCTGA CTTGGTTAAG 
AGGATCCGTG ACGCCATCCG CATGGGCTTG TCTGCGCGAC ACGTGCCCAG CCTCATCCTG 
GAAACCAAGG GCATCCCGTA TACGCTCAAC GGCAAGAAAG TGGAAGTTGC CGTCAAACAG 
ATCATCGCTG GAAAAGCCGT GGAGCAAGGA GGTGCTTTCT CGAACCCCGA GACCCTGGAT 
CTGTACCGGG ACATCCCTGA GCTGCAGGGC TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001306769.1
CDS	219..2114
Translation	MSKEERPGREEILECQVMWEPDSKKNTQMDRFRAAVGAACGLALESYDDLYHWSVESYSDFWAEFWKFSGIVFSRVYDEVVDTSKGIADVPEWFKGSRLNYAENLLRHKENDRVALYIAREGKEEIVKVTFEELRQEVALFAAAMRKMGVKKGDRVVGYLPNSEHAVEAMLAAASIGAIWSSTSPDFGVNGVLDRFSQIQPKLIFSVEAVVYNGKEHNHMEKLQQVVKGLPDLKKVVVIPYVSSRENIDLSKIPNSVFLDDFLATGTSEQAPQLEFEQLPFSHPLFIMFSSGTTGAPKCMVHSAGGTLIQHLKEHLLHGNMTSSDILLCYTTVGWMMWNWMVSLLATGAAMVLYDGSPLVPTPNVLWDLVDRIGITVLVTGAKWLSVLEEKAMKPGGTDIISCFMGHNFSLPVYKGEIQARNLGMAVEAWNEEGKAVWGESGELVCTKPIPCQPTHFWNDENGNKYRKAYFSKFPGIWAHGDYCRINPKTGGIVMLGRSDGTLNPNGVRFGSSEIYNIVESFEEVEDSLCVPQYNKYREERVILFLKMASGHAFQPDLVKRIRDAIRMGLSARHVPSLILETKGIPYTLNGKKVEVAVKQIIAGKAVEQGGAFSNPETLDLYRDIPELQGF

Target ORF information:

RefSeq Version	NM_001319840.1
Organism	Homo sapiens(human)
Definition	Homo sapiens acetoacetyl-CoA synthetase (AACS), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001319840.1

ORF Insert Sequence:

ATGTCCAAGG AGGAGCGCCC CGGTCGGGAG GAGATCCTGG AGTGCCAGGT GATGTGGGAG 
CCTGACAGTA AGAAGAACAC GCAGATGGAC CGCTTCCGGG CGGCTGTGGG CGCCGCCTGC 
GGCCTGGCGC TGGAGAGTTA TGATGACTTG TACCATTGGT CCGTTGAGTC ATATTCAGAC 
TTCTGGGCAG AGTTCTGGAA ATTCAGTGGA ATTGTCTTCT CACGTGTGTA TGATGAGGTT 
GTGGACACAT CGAAAGGAAT CGCAGATGTC CCCGAGTGGT TCAAAGGCAG TCGGCTCAAC 
TATGCAGAAA ACCTCCTGCG GCACAAAGAG AATGACAGAG TTGCCCTTTA CATTGCAAGG 
GAAGGCAAAG AGGAAATTGT GAAGGTGACT TTTGAAGAGC TGAGGCAAGA AGTGGCTTTG 
TTTGCAGCAG CAATGAGGAA AATGGGTGTG AAGAAAGGAG ATCGGGTTGT TGGTTATTTA 
CCCAACAGTG AGCACGCTGT CGAGGCGATG CTGGCTGCGG CAAGCATTGG TGCCATCTGG 
AGCTCCACGT CCCCGGACTT CGGTGTGAAT GGTGTGCTGG ACCGGTTTTC TCAAATTCAG 
CCAAAGCTCA TCTTCTCTGT GGAGGCTGTT GTCTATAATG GCAAAGAGCA CAACCACATG 
GAAAAGCTGC AGCAGGTGGT TAAAGGCCTA CCAGACTTGA AGAAAGTGGT GGTGATTCCT 
TATGTGTCCT CCAGAGAGAA CATAGACCTT TCAAAGATTC CAAACAGTGT GTTTCTGGAT 
GACTTTCTTG CCACCGGCAC CAGTGAGCAG GCCCCGCAGC TGGAGTTCGA GCAGCTGCCC 
TTCAGCCACC CACTGTTCAT CATGTTCTCA TCGGGCACCA CGGGCGCACC CAAGTGCATG 
GTGCATTCCG CTGGGGGCAC CCTCATCCAG CATCTGAAGG AGCACCTGCT GCACGGCAAC 
ATGACCAGCA GTGACATCCT CCTGTGCTAC ACCACGGTCG GCTGGATGAT GTGGAACTGG 
ATGGTGTCCC TTCTGGCCAC AGGAGCGGCC ATGGTCTTGT ACGATGGCTC CCCCCTGGTG 
CCCACGCCCA ATGTGCTCTG GGACCTGGTT GACAGGATAG GCATCACTGT CCTGGTAACT 
GGGGCCAAGT GGCTGTCAGT GCTGGAAGAG AAGGCCATGA AGCCGGGAGG CACCGACATC 
ATCTCCTGCT TCATGGGCCA CAATTTTTCT CTTCCTGTGT ATAAAGGGGA GATTCAGGCC 
CGGAACCTGG GCATGGCCGT GGAAGCGTGG AACGAGGAAG GAAAGGCGGT CTGGGGAGAG 
AGCGGCGAGC TGGTGTGTAC TAAGCCGATC CCTTGCCAGC CCACACACTT CTGGAACGAT 
GAGAACGGCA ACAAGTACAG GAAGGCGTAT TTCTCCAAAT TCCCAGGTAT CTGGGCTCAT 
GGCGACTACT GCAGAATCAA CCCCAAGACC GGGGGCATCG TCATGCTTGG CCGGAGTGAC 
GGCACCCTCA ACCCCAACGG GGTGCGGTTC GGCAGCTCGG AAATCTATAA CATTGTGGAA 
TCCTTCGAGG AGGTGGAGGA CAGCCTGTGT GTCCCCCAGT ATAACAAGTA CAGGGAGGAG 
AGGGTGATCC TCTTCCTGAA GATGGCCTCC GGGCACGCCT TCCAGCCTGA CTTGGTTAAG 
AGGATCCGTG ACGCCATCCG CATGGGCTTG TCTGCGCGAC ACGTGCCCAG CCTCATCCTG 
GAAACCAAGG GCATCCCGTA TACGCTCAAC GGCAAGAAAG TGGAAGTTGC CGTCAAACAG 
ATCATCGCTG GAAAAGCCGT GGAGCAAGGA GGTGCTTTCT CGAACCCCGA GACCCTGGAT 
CTGTACCGGG ACATCCCTGA GCTGCAGGGC TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AACS ( NM_001319840.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001306769.1 Homo sapiens acetoacetyl-CoA synthetase (AACS), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu46803
Clone ID Related Accession (Same CDS sequence)	NM_001319840.1 , NM_001319840.2
Accession Version	NM_001319840.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	acetoacetyl-CoA synthetase isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC122688.5. On May 31, 2019 this sequence version replaced NM_001319840.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.171002.1, SRR1803617.251472.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAAGG AGGAGCGCCC CGGTCGGGAG GAGATCCTGG AGTGCCAGGT GATGTGGGAG 
CCTGACAGTA AGAAGAACAC GCAGATGGAC CGCTTCCGGG CGGCTGTGGG CGCCGCCTGC 
GGCCTGGCGC TGGAGAGTTA TGATGACTTG TACCATTGGT CCGTTGAGTC ATATTCAGAC 
TTCTGGGCAG AGTTCTGGAA ATTCAGTGGA ATTGTCTTCT CACGTGTGTA TGATGAGGTT 
GTGGACACAT CGAAAGGAAT CGCAGATGTC CCCGAGTGGT TCAAAGGCAG TCGGCTCAAC 
TATGCAGAAA ACCTCCTGCG GCACAAAGAG AATGACAGAG TTGCCCTTTA CATTGCAAGG 
GAAGGCAAAG AGGAAATTGT GAAGGTGACT TTTGAAGAGC TGAGGCAAGA AGTGGCTTTG 
TTTGCAGCAG CAATGAGGAA AATGGGTGTG AAGAAAGGAG ATCGGGTTGT TGGTTATTTA 
CCCAACAGTG AGCACGCTGT CGAGGCGATG CTGGCTGCGG CAAGCATTGG TGCCATCTGG 
AGCTCCACGT CCCCGGACTT CGGTGTGAAT GGTGTGCTGG ACCGGTTTTC TCAAATTCAG 
CCAAAGCTCA TCTTCTCTGT GGAGGCTGTT GTCTATAATG GCAAAGAGCA CAACCACATG 
GAAAAGCTGC AGCAGGTGGT TAAAGGCCTA CCAGACTTGA AGAAAGTGGT GGTGATTCCT 
TATGTGTCCT CCAGAGAGAA CATAGACCTT TCAAAGATTC CAAACAGTGT GTTTCTGGAT 
GACTTTCTTG CCACCGGCAC CAGTGAGCAG GCCCCGCAGC TGGAGTTCGA GCAGCTGCCC 
TTCAGCCACC CACTGTTCAT CATGTTCTCA TCGGGCACCA CGGGCGCACC CAAGTGCATG 
GTGCATTCCG CTGGGGGCAC CCTCATCCAG CATCTGAAGG AGCACCTGCT GCACGGCAAC 
ATGACCAGCA GTGACATCCT CCTGTGCTAC ACCACGGTCG GCTGGATGAT GTGGAACTGG 
ATGGTGTCCC TTCTGGCCAC AGGAGCGGCC ATGGTCTTGT ACGATGGCTC CCCCCTGGTG 
CCCACGCCCA ATGTGCTCTG GGACCTGGTT GACAGGATAG GCATCACTGT CCTGGTAACT 
GGGGCCAAGT GGCTGTCAGT GCTGGAAGAG AAGGCCATGA AGCCGGGAGG CACCGACATC 
ATCTCCTGCT TCATGGGCCA CAATTTTTCT CTTCCTGTGT ATAAAGGGGA GATTCAGGCC 
CGGAACCTGG GCATGGCCGT GGAAGCGTGG AACGAGGAAG GAAAGGCGGT CTGGGGAGAG 
AGCGGCGAGC TGGTGTGTAC TAAGCCGATC CCTTGCCAGC CCACACACTT CTGGAACGAT 
GAGAACGGCA ACAAGTACAG GAAGGCGTAT TTCTCCAAAT TCCCAGGTAT CTGGGCTCAT 
GGCGACTACT GCAGAATCAA CCCCAAGACC GGGGGCATCG TCATGCTTGG CCGGAGTGAC 
GGCACCCTCA ACCCCAACGG GGTGCGGTTC GGCAGCTCGG AAATCTATAA CATTGTGGAA 
TCCTTCGAGG AGGTGGAGGA CAGCCTGTGT GTCCCCCAGT ATAACAAGTA CAGGGAGGAG 
AGGGTGATCC TCTTCCTGAA GATGGCCTCC GGGCACGCCT TCCAGCCTGA CTTGGTTAAG 
AGGATCCGTG ACGCCATCCG CATGGGCTTG TCTGCGCGAC ACGTGCCCAG CCTCATCCTG 
GAAACCAAGG GCATCCCGTA TACGCTCAAC GGCAAGAAAG TGGAAGTTGC CGTCAAACAG 
ATCATCGCTG GAAAAGCCGT GGAGCAAGGA GGTGCTTTCT CGAACCCCGA GACCCTGGAT 
CTGTACCGGG ACATCCCTGA GCTGCAGGGC TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001306769.1
CDS	151..2046
Translation	MSKEERPGREEILECQVMWEPDSKKNTQMDRFRAAVGAACGLALESYDDLYHWSVESYSDFWAEFWKFSGIVFSRVYDEVVDTSKGIADVPEWFKGSRLNYAENLLRHKENDRVALYIAREGKEEIVKVTFEELRQEVALFAAAMRKMGVKKGDRVVGYLPNSEHAVEAMLAAASIGAIWSSTSPDFGVNGVLDRFSQIQPKLIFSVEAVVYNGKEHNHMEKLQQVVKGLPDLKKVVVIPYVSSRENIDLSKIPNSVFLDDFLATGTSEQAPQLEFEQLPFSHPLFIMFSSGTTGAPKCMVHSAGGTLIQHLKEHLLHGNMTSSDILLCYTTVGWMMWNWMVSLLATGAAMVLYDGSPLVPTPNVLWDLVDRIGITVLVTGAKWLSVLEEKAMKPGGTDIISCFMGHNFSLPVYKGEIQARNLGMAVEAWNEEGKAVWGESGELVCTKPIPCQPTHFWNDENGNKYRKAYFSKFPGIWAHGDYCRINPKTGGIVMLGRSDGTLNPNGVRFGSSEIYNIVESFEEVEDSLCVPQYNKYREERVILFLKMASGHAFQPDLVKRIRDAIRMGLSARHVPSLILETKGIPYTLNGKKVEVAVKQIIAGKAVEQGGAFSNPETLDLYRDIPELQGF

Target ORF information:

RefSeq Version	NM_001319840.2
Organism	Homo sapiens(human)
Definition	Homo sapiens acetoacetyl-CoA synthetase (AACS), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001319840.2

ORF Insert Sequence:

ATGTCCAAGG AGGAGCGCCC CGGTCGGGAG GAGATCCTGG AGTGCCAGGT GATGTGGGAG 
CCTGACAGTA AGAAGAACAC GCAGATGGAC CGCTTCCGGG CGGCTGTGGG CGCCGCCTGC 
GGCCTGGCGC TGGAGAGTTA TGATGACTTG TACCATTGGT CCGTTGAGTC ATATTCAGAC 
TTCTGGGCAG AGTTCTGGAA ATTCAGTGGA ATTGTCTTCT CACGTGTGTA TGATGAGGTT 
GTGGACACAT CGAAAGGAAT CGCAGATGTC CCCGAGTGGT TCAAAGGCAG TCGGCTCAAC 
TATGCAGAAA ACCTCCTGCG GCACAAAGAG AATGACAGAG TTGCCCTTTA CATTGCAAGG 
GAAGGCAAAG AGGAAATTGT GAAGGTGACT TTTGAAGAGC TGAGGCAAGA AGTGGCTTTG 
TTTGCAGCAG CAATGAGGAA AATGGGTGTG AAGAAAGGAG ATCGGGTTGT TGGTTATTTA 
CCCAACAGTG AGCACGCTGT CGAGGCGATG CTGGCTGCGG CAAGCATTGG TGCCATCTGG 
AGCTCCACGT CCCCGGACTT CGGTGTGAAT GGTGTGCTGG ACCGGTTTTC TCAAATTCAG 
CCAAAGCTCA TCTTCTCTGT GGAGGCTGTT GTCTATAATG GCAAAGAGCA CAACCACATG 
GAAAAGCTGC AGCAGGTGGT TAAAGGCCTA CCAGACTTGA AGAAAGTGGT GGTGATTCCT 
TATGTGTCCT CCAGAGAGAA CATAGACCTT TCAAAGATTC CAAACAGTGT GTTTCTGGAT 
GACTTTCTTG CCACCGGCAC CAGTGAGCAG GCCCCGCAGC TGGAGTTCGA GCAGCTGCCC 
TTCAGCCACC CACTGTTCAT CATGTTCTCA TCGGGCACCA CGGGCGCACC CAAGTGCATG 
GTGCATTCCG CTGGGGGCAC CCTCATCCAG CATCTGAAGG AGCACCTGCT GCACGGCAAC 
ATGACCAGCA GTGACATCCT CCTGTGCTAC ACCACGGTCG GCTGGATGAT GTGGAACTGG 
ATGGTGTCCC TTCTGGCCAC AGGAGCGGCC ATGGTCTTGT ACGATGGCTC CCCCCTGGTG 
CCCACGCCCA ATGTGCTCTG GGACCTGGTT GACAGGATAG GCATCACTGT CCTGGTAACT 
GGGGCCAAGT GGCTGTCAGT GCTGGAAGAG AAGGCCATGA AGCCGGGAGG CACCGACATC 
ATCTCCTGCT TCATGGGCCA CAATTTTTCT CTTCCTGTGT ATAAAGGGGA GATTCAGGCC 
CGGAACCTGG GCATGGCCGT GGAAGCGTGG AACGAGGAAG GAAAGGCGGT CTGGGGAGAG 
AGCGGCGAGC TGGTGTGTAC TAAGCCGATC CCTTGCCAGC CCACACACTT CTGGAACGAT 
GAGAACGGCA ACAAGTACAG GAAGGCGTAT TTCTCCAAAT TCCCAGGTAT CTGGGCTCAT 
GGCGACTACT GCAGAATCAA CCCCAAGACC GGGGGCATCG TCATGCTTGG CCGGAGTGAC 
GGCACCCTCA ACCCCAACGG GGTGCGGTTC GGCAGCTCGG AAATCTATAA CATTGTGGAA 
TCCTTCGAGG AGGTGGAGGA CAGCCTGTGT GTCCCCCAGT ATAACAAGTA CAGGGAGGAG 
AGGGTGATCC TCTTCCTGAA GATGGCCTCC GGGCACGCCT TCCAGCCTGA CTTGGTTAAG 
AGGATCCGTG ACGCCATCCG CATGGGCTTG TCTGCGCGAC ACGTGCCCAG CCTCATCCTG 
GAAACCAAGG GCATCCCGTA TACGCTCAAC GGCAAGAAAG TGGAAGTTGC CGTCAAACAG 
ATCATCGCTG GAAAAGCCGT GGAGCAAGGA GGTGCTTTCT CGAACCCCGA GACCCTGGAT 
CTGTACCGGG ACATCCCTGA GCTGCAGGGC TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AACS ( NM_001319839.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001306768.1 Homo sapiens acetoacetyl-CoA synthetase (AACS), transcript variant 2, mRNA.

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CloneID	OHu46804
Clone ID Related Accession (Same CDS sequence)	NM_001319839.1 , NM_001319839.2
Accession Version	NM_001319839.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	acetoacetyl-CoA synthetase isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY018418.1, BC041000.1, BC051862.1, BC040490.1 and BM975973.1. On Feb 5, 2016 this sequence version replaced XM_005253610.2. Transcript Variant: This variant (2) lacks an alternate exon in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (2) has a distinct, shorter C-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC041000.1, SRR1803616.201580.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAAGG AGGAGCGCCC CGGTCGGGAG GAGATCCTGG AGTGCCAGGT GATGTGGGAG 
CCTGACAGTA AGAAGAACAC GCAGATGGAC CGCTTCCGGG CGGCTGTGGG CGCCGCCTGC 
GGCCTGGCGC TGGAGAGTTA TGATGACTTG TACCATTGGT CCGTTGAGTC ATATTCAGAC 
TTCTGGGCAG AGTTCTGGAA ATTCAGTGGA ATTGTCTTCT CACGTGTGTA TGATGAGGTT 
GTGGACACAT CGAAAGGAAT CGCAGATGTC CCCGAGTGGT TCAAAGGCAG TCGGCTCAAC 
TATGCAGAAA ACCTCCTGCG GCACAAAGAG AATGACAGAG TTGCCCTTTA CATTGCAAGG 
GAAGGCAAAG AGGAAATTGT GAAGGTGACT TTTGAAGAGC TGAGGCAAGA AGTGGCTTTG 
TTTGCAGCAG CAATGAGGAA AATGGGTGTG AAGAAAGGAG ATCGGGTTGT TGGTTATTTA 
CCCAACAGTG AGCACGCTGT CGAGGCGATG CTGGCTGCGG CAAGCATTGG TGCCATCTGG 
AGCTCCACGT CCCCGGACTT CGGTGTGAAT GGTGTGCTGG ACCGGTTTTC TCAAATTCAG 
CCAAAGCTCA TCTTCTCTGT GGAGGCTGTT GTCTATAATG GCAAAGAGCA CAACCACATG 
GAAAAGCTGC AGCAGGTGGT TAAAGGCCTA CCAGACTTGA AGAAAGTGGT GGTGATTCCT 
TATGTGTCCT CCAGAGAGAA CATAGACCTT TCAAAGATTC CAAACAGTGT GTTTCTGGAT 
GACTTTCTTG CCACCGGCAC CAGTGAGCAG GCCCCGCAGC TGGAGTTCGA GCAGCTGCCC 
TTCAGCCACC CACTGTTCAT CATGTTCTCA TCGGGCACCA CGGGCGCACC CAAGTGCATG 
GTGCATTCCG CTGGGGGCAC CCTCATCCAG CATCTGAAGG AGCACCTGCT GCACGGCAAC 
ATGACCAGCA GTGACATCCT CCTGTGCTAC ACCACGGTCG GCTGGATGAT GTGGAACTGG 
ATGGTGTCCC TTCTGGCCAC AGGAGCGGCC ATGGTCTTGT ACGATGGCTC CCCCCTGGTG 
CCCACGCCCA ATGTGCTCTG GGACCTGGTT GACAGGATAG GCATCACTGT CCTGGTAACT 
GGGGCCAAGT GGCTGTCAGT GCTGGAAGAG AAGGCCATGA AGCCGGTGGA AACCCACAGT 
CTCCAGATGC TCCACACGAT CCTGTCCACT GGCTCCCCAC TGAAAGCCCA GAGCTACGAG 
TATGTCTACA GGTGCATCAA GAGCAGCATC CTCCTGGGCT CCATCTCAGG AGGCACCGAC 
ATCATCTCCT GCTTCATGGG CCACAATTTT TCTCTTCCTG TGTATAAAGG GGAGATTCAG 
GCCCGGAACC TGGGCATGGC CGTGGAAGCG TGGAACGAGG AAGGAAAGGC GGTCTGGGGA 
GAGAGCGGCG AGCTGGTGTG TACTAAGCCG ATCCCTTGCC AGCCCACACA CTTCTGGAAC 
GATGAGAACG GCAACAAGTA CAGGAAGGCG TATTTCTCCA AATTCCCAGG TATCTGGGCT 
CATGGCGACT ACTGCAGAAT CAACCCCAAG ACCGGGGGCA TCGTCATGCT TGGCCGGAGT 
GACGGCACCC TCAACCCCAA CGGGGTGCGG TTCGGCAGCT CGGAAATCTA TAACATTGTA 
TACGCTCAAC GGCAAGAAAG TGGAAGTTGC CGTCAAACAG ATCATCGCTG GAAAAGCCGT 
GGAGCAAGGA GGTGCTTTCT CGAACCCCGA GACCCTGGAT CTGTACCGGG ACATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001306768.1
CDS	219..2018
Translation	MSKEERPGREEILECQVMWEPDSKKNTQMDRFRAAVGAACGLALESYDDLYHWSVESYSDFWAEFWKFSGIVFSRVYDEVVDTSKGIADVPEWFKGSRLNYAENLLRHKENDRVALYIAREGKEEIVKVTFEELRQEVALFAAAMRKMGVKKGDRVVGYLPNSEHAVEAMLAAASIGAIWSSTSPDFGVNGVLDRFSQIQPKLIFSVEAVVYNGKEHNHMEKLQQVVKGLPDLKKVVVIPYVSSRENIDLSKIPNSVFLDDFLATGTSEQAPQLEFEQLPFSHPLFIMFSSGTTGAPKCMVHSAGGTLIQHLKEHLLHGNMTSSDILLCYTTVGWMMWNWMVSLLATGAAMVLYDGSPLVPTPNVLWDLVDRIGITVLVTGAKWLSVLEEKAMKPVETHSLQMLHTILSTGSPLKAQSYEYVYRCIKSSILLGSISGGTDIISCFMGHNFSLPVYKGEIQARNLGMAVEAWNEEGKAVWGESGELVCTKPIPCQPTHFWNDENGNKYRKAYFSKFPGIWAHGDYCRINPKTGGIVMLGRSDGTLNPNGVRFGSSEIYNIVYAQRQESGSCRQTDHRWKSRGARRCFLEPRDPGSVPGHP

Target ORF information:

RefSeq Version	NM_001319839.1
Organism	Homo sapiens(human)
Definition	Homo sapiens acetoacetyl-CoA synthetase (AACS), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001319839.1

ORF Insert Sequence:

ATGTCCAAGG AGGAGCGCCC CGGTCGGGAG GAGATCCTGG AGTGCCAGGT GATGTGGGAG 
CCTGACAGTA AGAAGAACAC GCAGATGGAC CGCTTCCGGG CGGCTGTGGG CGCCGCCTGC 
GGCCTGGCGC TGGAGAGTTA TGATGACTTG TACCATTGGT CCGTTGAGTC ATATTCAGAC 
TTCTGGGCAG AGTTCTGGAA ATTCAGTGGA ATTGTCTTCT CACGTGTGTA TGATGAGGTT 
GTGGACACAT CGAAAGGAAT CGCAGATGTC CCCGAGTGGT TCAAAGGCAG TCGGCTCAAC 
TATGCAGAAA ACCTCCTGCG GCACAAAGAG AATGACAGAG TTGCCCTTTA CATTGCAAGG 
GAAGGCAAAG AGGAAATTGT GAAGGTGACT TTTGAAGAGC TGAGGCAAGA AGTGGCTTTG 
TTTGCAGCAG CAATGAGGAA AATGGGTGTG AAGAAAGGAG ATCGGGTTGT TGGTTATTTA 
CCCAACAGTG AGCACGCTGT CGAGGCGATG CTGGCTGCGG CAAGCATTGG TGCCATCTGG 
AGCTCCACGT CCCCGGACTT CGGTGTGAAT GGTGTGCTGG ACCGGTTTTC TCAAATTCAG 
CCAAAGCTCA TCTTCTCTGT GGAGGCTGTT GTCTATAATG GCAAAGAGCA CAACCACATG 
GAAAAGCTGC AGCAGGTGGT TAAAGGCCTA CCAGACTTGA AGAAAGTGGT GGTGATTCCT 
TATGTGTCCT CCAGAGAGAA CATAGACCTT TCAAAGATTC CAAACAGTGT GTTTCTGGAT 
GACTTTCTTG CCACCGGCAC CAGTGAGCAG GCCCCGCAGC TGGAGTTCGA GCAGCTGCCC 
TTCAGCCACC CACTGTTCAT CATGTTCTCA TCGGGCACCA CGGGCGCACC CAAGTGCATG 
GTGCATTCCG CTGGGGGCAC CCTCATCCAG CATCTGAAGG AGCACCTGCT GCACGGCAAC 
ATGACCAGCA GTGACATCCT CCTGTGCTAC ACCACGGTCG GCTGGATGAT GTGGAACTGG 
ATGGTGTCCC TTCTGGCCAC AGGAGCGGCC ATGGTCTTGT ACGATGGCTC CCCCCTGGTG 
CCCACGCCCA ATGTGCTCTG GGACCTGGTT GACAGGATAG GCATCACTGT CCTGGTAACT 
GGGGCCAAGT GGCTGTCAGT GCTGGAAGAG AAGGCCATGA AGCCGGTGGA AACCCACAGT 
CTCCAGATGC TCCACACGAT CCTGTCCACT GGCTCCCCAC TGAAAGCCCA GAGCTACGAG 
TATGTCTACA GGTGCATCAA GAGCAGCATC CTCCTGGGCT CCATCTCAGG AGGCACCGAC 
ATCATCTCCT GCTTCATGGG CCACAATTTT TCTCTTCCTG TGTATAAAGG GGAGATTCAG 
GCCCGGAACC TGGGCATGGC CGTGGAAGCG TGGAACGAGG AAGGAAAGGC GGTCTGGGGA 
GAGAGCGGCG AGCTGGTGTG TACTAAGCCG ATCCCTTGCC AGCCCACACA CTTCTGGAAC 
GATGAGAACG GCAACAAGTA CAGGAAGGCG TATTTCTCCA AATTCCCAGG TATCTGGGCT 
CATGGCGACT ACTGCAGAAT CAACCCCAAG ACCGGGGGCA TCGTCATGCT TGGCCGGAGT 
GACGGCACCC TCAACCCCAA CGGGGTGCGG TTCGGCAGCT CGGAAATCTA TAACATTGTA 
TACGCTCAAC GGCAAGAAAG TGGAAGTTGC CGTCAAACAG ATCATCGCTG GAAAAGCCGT 
GGAGCAAGGA GGTGCTTTCT CGAACCCCGA GACCCTGGAT CTGTACCGGG ACATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AACS ( NM_001319839.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001306768.1 Homo sapiens acetoacetyl-CoA synthetase (AACS), transcript variant 2, mRNA.

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CloneID	OHu46804
Clone ID Related Accession (Same CDS sequence)	NM_001319839.1 , NM_001319839.2
Accession Version	NM_001319839.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Homo sapiens(human)
Product	acetoacetyl-CoA synthetase isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY018418.1, BC041000.1, BC051862.1, BC040490.1 and BM975973.1. On May 31, 2019 this sequence version replaced NM_001319839.1. Transcript Variant: This variant (2) lacks an alternate exon in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (2) has a distinct, shorter C-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.82294.1, SRR1803616.201580.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAAGG AGGAGCGCCC CGGTCGGGAG GAGATCCTGG AGTGCCAGGT GATGTGGGAG 
CCTGACAGTA AGAAGAACAC GCAGATGGAC CGCTTCCGGG CGGCTGTGGG CGCCGCCTGC 
GGCCTGGCGC TGGAGAGTTA TGATGACTTG TACCATTGGT CCGTTGAGTC ATATTCAGAC 
TTCTGGGCAG AGTTCTGGAA ATTCAGTGGA ATTGTCTTCT CACGTGTGTA TGATGAGGTT 
GTGGACACAT CGAAAGGAAT CGCAGATGTC CCCGAGTGGT TCAAAGGCAG TCGGCTCAAC 
TATGCAGAAA ACCTCCTGCG GCACAAAGAG AATGACAGAG TTGCCCTTTA CATTGCAAGG 
GAAGGCAAAG AGGAAATTGT GAAGGTGACT TTTGAAGAGC TGAGGCAAGA AGTGGCTTTG 
TTTGCAGCAG CAATGAGGAA AATGGGTGTG AAGAAAGGAG ATCGGGTTGT TGGTTATTTA 
CCCAACAGTG AGCACGCTGT CGAGGCGATG CTGGCTGCGG CAAGCATTGG TGCCATCTGG 
AGCTCCACGT CCCCGGACTT CGGTGTGAAT GGTGTGCTGG ACCGGTTTTC TCAAATTCAG 
CCAAAGCTCA TCTTCTCTGT GGAGGCTGTT GTCTATAATG GCAAAGAGCA CAACCACATG 
GAAAAGCTGC AGCAGGTGGT TAAAGGCCTA CCAGACTTGA AGAAAGTGGT GGTGATTCCT 
TATGTGTCCT CCAGAGAGAA CATAGACCTT TCAAAGATTC CAAACAGTGT GTTTCTGGAT 
GACTTTCTTG CCACCGGCAC CAGTGAGCAG GCCCCGCAGC TGGAGTTCGA GCAGCTGCCC 
TTCAGCCACC CACTGTTCAT CATGTTCTCA TCGGGCACCA CGGGCGCACC CAAGTGCATG 
GTGCATTCCG CTGGGGGCAC CCTCATCCAG CATCTGAAGG AGCACCTGCT GCACGGCAAC 
ATGACCAGCA GTGACATCCT CCTGTGCTAC ACCACGGTCG GCTGGATGAT GTGGAACTGG 
ATGGTGTCCC TTCTGGCCAC AGGAGCGGCC ATGGTCTTGT ACGATGGCTC CCCCCTGGTG 
CCCACGCCCA ATGTGCTCTG GGACCTGGTT GACAGGATAG GCATCACTGT CCTGGTAACT 
GGGGCCAAGT GGCTGTCAGT GCTGGAAGAG AAGGCCATGA AGCCGGTGGA AACCCACAGT 
CTCCAGATGC TCCACACGAT CCTGTCCACT GGCTCCCCAC TGAAAGCCCA GAGCTACGAG 
TATGTCTACA GGTGCATCAA GAGCAGCATC CTCCTGGGCT CCATCTCAGG AGGCACCGAC 
ATCATCTCCT GCTTCATGGG CCACAATTTT TCTCTTCCTG TGTATAAAGG GGAGATTCAG 
GCCCGGAACC TGGGCATGGC CGTGGAAGCG TGGAACGAGG AAGGAAAGGC GGTCTGGGGA 
GAGAGCGGCG AGCTGGTGTG TACTAAGCCG ATCCCTTGCC AGCCCACACA CTTCTGGAAC 
GATGAGAACG GCAACAAGTA CAGGAAGGCG TATTTCTCCA AATTCCCAGG TATCTGGGCT 
CATGGCGACT ACTGCAGAAT CAACCCCAAG ACCGGGGGCA TCGTCATGCT TGGCCGGAGT 
GACGGCACCC TCAACCCCAA CGGGGTGCGG TTCGGCAGCT CGGAAATCTA TAACATTGTA 
TACGCTCAAC GGCAAGAAAG TGGAAGTTGC CGTCAAACAG ATCATCGCTG GAAAAGCCGT 
GGAGCAAGGA GGTGCTTTCT CGAACCCCGA GACCCTGGAT CTGTACCGGG ACATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001306768.1
CDS	151..1950
Translation	MSKEERPGREEILECQVMWEPDSKKNTQMDRFRAAVGAACGLALESYDDLYHWSVESYSDFWAEFWKFSGIVFSRVYDEVVDTSKGIADVPEWFKGSRLNYAENLLRHKENDRVALYIAREGKEEIVKVTFEELRQEVALFAAAMRKMGVKKGDRVVGYLPNSEHAVEAMLAAASIGAIWSSTSPDFGVNGVLDRFSQIQPKLIFSVEAVVYNGKEHNHMEKLQQVVKGLPDLKKVVVIPYVSSRENIDLSKIPNSVFLDDFLATGTSEQAPQLEFEQLPFSHPLFIMFSSGTTGAPKCMVHSAGGTLIQHLKEHLLHGNMTSSDILLCYTTVGWMMWNWMVSLLATGAAMVLYDGSPLVPTPNVLWDLVDRIGITVLVTGAKWLSVLEEKAMKPVETHSLQMLHTILSTGSPLKAQSYEYVYRCIKSSILLGSISGGTDIISCFMGHNFSLPVYKGEIQARNLGMAVEAWNEEGKAVWGESGELVCTKPIPCQPTHFWNDENGNKYRKAYFSKFPGIWAHGDYCRINPKTGGIVMLGRSDGTLNPNGVRFGSSEIYNIVYAQRQESGSCRQTDHRWKSRGARRCFLEPRDPGSVPGHP

Target ORF information:

RefSeq Version	NM_001319839.2
Organism	Homo sapiens(human)
Definition	Homo sapiens acetoacetyl-CoA synthetase (AACS), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001319839.2

ORF Insert Sequence:

ATGTCCAAGG AGGAGCGCCC CGGTCGGGAG GAGATCCTGG AGTGCCAGGT GATGTGGGAG 
CCTGACAGTA AGAAGAACAC GCAGATGGAC CGCTTCCGGG CGGCTGTGGG CGCCGCCTGC 
GGCCTGGCGC TGGAGAGTTA TGATGACTTG TACCATTGGT CCGTTGAGTC ATATTCAGAC 
TTCTGGGCAG AGTTCTGGAA ATTCAGTGGA ATTGTCTTCT CACGTGTGTA TGATGAGGTT 
GTGGACACAT CGAAAGGAAT CGCAGATGTC CCCGAGTGGT TCAAAGGCAG TCGGCTCAAC 
TATGCAGAAA ACCTCCTGCG GCACAAAGAG AATGACAGAG TTGCCCTTTA CATTGCAAGG 
GAAGGCAAAG AGGAAATTGT GAAGGTGACT TTTGAAGAGC TGAGGCAAGA AGTGGCTTTG 
TTTGCAGCAG CAATGAGGAA AATGGGTGTG AAGAAAGGAG ATCGGGTTGT TGGTTATTTA 
CCCAACAGTG AGCACGCTGT CGAGGCGATG CTGGCTGCGG CAAGCATTGG TGCCATCTGG 
AGCTCCACGT CCCCGGACTT CGGTGTGAAT GGTGTGCTGG ACCGGTTTTC TCAAATTCAG 
CCAAAGCTCA TCTTCTCTGT GGAGGCTGTT GTCTATAATG GCAAAGAGCA CAACCACATG 
GAAAAGCTGC AGCAGGTGGT TAAAGGCCTA CCAGACTTGA AGAAAGTGGT GGTGATTCCT 
TATGTGTCCT CCAGAGAGAA CATAGACCTT TCAAAGATTC CAAACAGTGT GTTTCTGGAT 
GACTTTCTTG CCACCGGCAC CAGTGAGCAG GCCCCGCAGC TGGAGTTCGA GCAGCTGCCC 
TTCAGCCACC CACTGTTCAT CATGTTCTCA TCGGGCACCA CGGGCGCACC CAAGTGCATG 
GTGCATTCCG CTGGGGGCAC CCTCATCCAG CATCTGAAGG AGCACCTGCT GCACGGCAAC 
ATGACCAGCA GTGACATCCT CCTGTGCTAC ACCACGGTCG GCTGGATGAT GTGGAACTGG 
ATGGTGTCCC TTCTGGCCAC AGGAGCGGCC ATGGTCTTGT ACGATGGCTC CCCCCTGGTG 
CCCACGCCCA ATGTGCTCTG GGACCTGGTT GACAGGATAG GCATCACTGT CCTGGTAACT 
GGGGCCAAGT GGCTGTCAGT GCTGGAAGAG AAGGCCATGA AGCCGGTGGA AACCCACAGT 
CTCCAGATGC TCCACACGAT CCTGTCCACT GGCTCCCCAC TGAAAGCCCA GAGCTACGAG 
TATGTCTACA GGTGCATCAA GAGCAGCATC CTCCTGGGCT CCATCTCAGG AGGCACCGAC 
ATCATCTCCT GCTTCATGGG CCACAATTTT TCTCTTCCTG TGTATAAAGG GGAGATTCAG 
GCCCGGAACC TGGGCATGGC CGTGGAAGCG TGGAACGAGG AAGGAAAGGC GGTCTGGGGA 
GAGAGCGGCG AGCTGGTGTG TACTAAGCCG ATCCCTTGCC AGCCCACACA CTTCTGGAAC 
GATGAGAACG GCAACAAGTA CAGGAAGGCG TATTTCTCCA AATTCCCAGG TATCTGGGCT 
CATGGCGACT ACTGCAGAAT CAACCCCAAG ACCGGGGGCA TCGTCATGCT TGGCCGGAGT 
GACGGCACCC TCAACCCCAA CGGGGTGCGG TTCGGCAGCT CGGAAATCTA TAACATTGTA 
TACGCTCAAC GGCAAGAAAG TGGAAGTTGC CGTCAAACAG ATCATCGCTG GAAAAGCCGT 
GGAGCAAGGA GGTGCTTTCT CGAACCCCGA GACCCTGGAT CTGTACCGGG ACATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AACS ( XM_011538692.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011536994.1 Homo sapiens acetoacetyl-CoA synthetase (AACS), transcript variant X1, mRNA.

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CloneID	OHu71674
Clone ID Related Accession (Same CDS sequence)	XM_011538692.2
Accession Version	XM_011538692.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1695bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	acetoacetyl-CoA synthetase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011538692.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAC GTCTCGACGT ACCATATGGG GCTGGGGAAG GCAAAGAGGA AATTGTGAAG 
GTGACTTTTG AAGAGCTGAG GCAAGAAGTG GCTTTGTTTG CAGCAGCAAT GAGGAAAATG 
GGTGTGAAGA AAGGAGATCG GGTTGTTGGT TATTTACCCA ACAGTGAGCA CGCTGTCGAG 
GCGATGCTGG CTGCGGCAAG CATTGGTGCC ATCTGGAGCT CCACGTCCCC GGACTTCGGT 
GTGAATGGTG TGCTGGACCG GTTTTCTCAA ATTCAGCCAA AGCTCATCTT CTCTGTGGAG 
GCTGTTGTCT ATAATGGCAA AGAGCACAAC CACATGGAAA AGCTGCAGCA GGTGGTTAAA 
GGCCTACCAG ACTTGAAGAA AGTGGTGGTG ATTCCTTATG TGTCCTCCAG AGAGAACATA 
GACCTTTCAA AGATTCCAAA CAGTGTGTTT CTGGATGACT TTCTTGCCAC CGGCACCAGT 
GAGCAGGCCC CGCAGCTGGA GTTCGAGCAG CTGCCCTTCA GCCACCCACT GTTCATCATG 
TTCTCATCGG GCACCACGGG CGCACCCAAG TGCATGGTGC ATTCCGCTGG GGGCACCCTC 
ATCCAGCATC TGAAGGAGCA CCTGCTGCAC GGCAACATGA CCAGCAGTGA CATCCTCCTG 
TGCTACACCA CGGTCGGCTG GATGATGTGG AACTGGATGG TGTCCCTTCT GGCCACAGGA 
GCGGCCATGG TCTTGTACGA TGGCTCCCCC CTGGTGCCCA CGCCCAATGT GCTCTGGGAC 
CTGGTTGACA GGATAGGCAT CACTGTCCTG GTAACTGGGG CCAAGTGGCT GTCAGTGCTG 
GAAGAGAAGG CCATGAAGCC GGTGGAAACC CACAGTCTCC AGATGCTCCA CACGATCCTG 
TCCACTGGCT CCCCACTGAA AGCCCAGAGC TACGAGTATG TCTACAGGTG CATCAAGAGC 
AGCATCCTCC TGGGCTCCAT CTCAGGAGGC ACCGACATCA TCTCCTGCTT CATGGGCCAC 
AATTTTTCTC TTCCTGTGTA TAAAGGGGAG ATTCAGGCCC GGAACCTGGG CATGGCCGTG 
GAAGCGTGGA ACGAGGAAGG AAAGGCGGTC TGGGGAGAGA GCGGCGAGCT GGTGTGTACT 
AAGCCGATCC CTTGCCAGCC CACACACTTC TGGAACGATG AGAACGGCAA CAAGTACAGG 
AAGGCGTATT TCTCCAAATT CCCAGGTATC TGGGCTCATG GCGACTACTG CAGAATCAAC 
CCCAAGACCG GGGGCATCGT CATGCTTGGC CGGAGTGACG GCACCCTCAA CCCCAACGGG 
GTGCGGTTCG GCAGCTCGGA AATCTATAAC ATTGTGGAAT CCTTCGAGGA GGTGGAGGAC 
AGCCTGTGTG TCCCCCAGTA TAACAAGTAC AGGGAGGAGA GGGTGATCCT CTTCCTGAAG 
ATGGCCTCCG GGCACGCCTT CCAGCCTGAC TTGGTTAAGA GGATCCGTGA CGCCATCCGC 
ATGGGCTTGT CTGCGCGACA CGTGCCCAGC CTCATCCTGG AAACCAAGGG CATCCCGTAT 
ACGCTCAACG GCAAGAAAGT GGAAGTTGCC GTCAAACAGA TCATCGCTGG AAAAGCCGTG 
GAGCAAGGAG GTGCTTTCTC GAACCCCGAG ACCCTGGATC TGTACCGGGA CATCCCTGAG 
CTGCAGGGCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011536994.1
CDS	54..1748
Translation	MEQRLDVPYGAGEGKEEIVKVTFEELRQEVALFAAAMRKMGVKKGDRVVGYLPNSEHAVEAMLAAASIGAIWSSTSPDFGVNGVLDRFSQIQPKLIFSVEAVVYNGKEHNHMEKLQQVVKGLPDLKKVVVIPYVSSRENIDLSKIPNSVFLDDFLATGTSEQAPQLEFEQLPFSHPLFIMFSSGTTGAPKCMVHSAGGTLIQHLKEHLLHGNMTSSDILLCYTTVGWMMWNWMVSLLATGAAMVLYDGSPLVPTPNVLWDLVDRIGITVLVTGAKWLSVLEEKAMKPVETHSLQMLHTILSTGSPLKAQSYEYVYRCIKSSILLGSISGGTDIISCFMGHNFSLPVYKGEIQARNLGMAVEAWNEEGKAVWGESGELVCTKPIPCQPTHFWNDENGNKYRKAYFSKFPGIWAHGDYCRINPKTGGIVMLGRSDGTLNPNGVRFGSSEIYNIVESFEEVEDSLCVPQYNKYREERVILFLKMASGHAFQPDLVKRIRDAIRMGLSARHVPSLILETKGIPYTLNGKKVEVAVKQIIAGKAVEQGGAFSNPETLDLYRDIPELQGF

Target ORF information:

RefSeq Version	XM_011538692.2
Organism	Homo sapiens(human)
Definition	Homo sapiens acetoacetyl-CoA synthetase (AACS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011538692.2

ORF Insert Sequence:

ATGGAGCAAC GTCTCGACGT ACCATATGGG GCTGGGGAAG GCAAAGAGGA AATTGTGAAG 
GTGACTTTTG AAGAGCTGAG GCAAGAAGTG GCTTTGTTTG CAGCAGCAAT GAGGAAAATG 
GGTGTGAAGA AAGGAGATCG GGTTGTTGGT TATTTACCCA ACAGTGAGCA CGCTGTCGAG 
GCGATGCTGG CTGCGGCAAG CATTGGTGCC ATCTGGAGCT CCACGTCCCC GGACTTCGGT 
GTGAATGGTG TGCTGGACCG GTTTTCTCAA ATTCAGCCAA AGCTCATCTT CTCTGTGGAG 
GCTGTTGTCT ATAATGGCAA AGAGCACAAC CACATGGAAA AGCTGCAGCA GGTGGTTAAA 
GGCCTACCAG ACTTGAAGAA AGTGGTGGTG ATTCCTTATG TGTCCTCCAG AGAGAACATA 
GACCTTTCAA AGATTCCAAA CAGTGTGTTT CTGGATGACT TTCTTGCCAC CGGCACCAGT 
GAGCAGGCCC CGCAGCTGGA GTTCGAGCAG CTGCCCTTCA GCCACCCACT GTTCATCATG 
TTCTCATCGG GCACCACGGG CGCACCCAAG TGCATGGTGC ATTCCGCTGG GGGCACCCTC 
ATCCAGCATC TGAAGGAGCA CCTGCTGCAC GGCAACATGA CCAGCAGTGA CATCCTCCTG 
TGCTACACCA CGGTCGGCTG GATGATGTGG AACTGGATGG TGTCCCTTCT GGCCACAGGA 
GCGGCCATGG TCTTGTACGA TGGCTCCCCC CTGGTGCCCA CGCCCAATGT GCTCTGGGAC 
CTGGTTGACA GGATAGGCAT CACTGTCCTG GTAACTGGGG CCAAGTGGCT GTCAGTGCTG 
GAAGAGAAGG CCATGAAGCC GGTGGAAACC CACAGTCTCC AGATGCTCCA CACGATCCTG 
TCCACTGGCT CCCCACTGAA AGCCCAGAGC TACGAGTATG TCTACAGGTG CATCAAGAGC 
AGCATCCTCC TGGGCTCCAT CTCAGGAGGC ACCGACATCA TCTCCTGCTT CATGGGCCAC 
AATTTTTCTC TTCCTGTGTA TAAAGGGGAG ATTCAGGCCC GGAACCTGGG CATGGCCGTG 
GAAGCGTGGA ACGAGGAAGG AAAGGCGGTC TGGGGAGAGA GCGGCGAGCT GGTGTGTACT 
AAGCCGATCC CTTGCCAGCC CACACACTTC TGGAACGATG AGAACGGCAA CAAGTACAGG 
AAGGCGTATT TCTCCAAATT CCCAGGTATC TGGGCTCATG GCGACTACTG CAGAATCAAC 
CCCAAGACCG GGGGCATCGT CATGCTTGGC CGGAGTGACG GCACCCTCAA CCCCAACGGG 
GTGCGGTTCG GCAGCTCGGA AATCTATAAC ATTGTGGAAT CCTTCGAGGA GGTGGAGGAC 
AGCCTGTGTG TCCCCCAGTA TAACAAGTAC AGGGAGGAGA GGGTGATCCT CTTCCTGAAG 
ATGGCCTCCG GGCACGCCTT CCAGCCTGAC TTGGTTAAGA GGATCCGTGA CGCCATCCGC 
ATGGGCTTGT CTGCGCGACA CGTGCCCAGC CTCATCCTGG AAACCAAGGG CATCCCGTAT 
ACGCTCAACG GCAAGAAAGT GGAAGTTGCC GTCAAACAGA TCATCGCTGG AAAAGCCGTG 
GAGCAAGGAG GTGCTTTCTC GAACCCCGAG ACCCTGGATC TGTACCGGGA CATCCCTGAG 
CTGCAGGGCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcriptional regulation of the human acetoacetyl-CoA synthetase gene by PPARgamma.
The Biochemical journal427(2)255-64(2010 Mar)
Aguil? F,Camarero N,Relat J,Marrero PF,Haro D

Evidence for 26 distinct acyl-coenzyme A synthetase genes in the human genome.
Journal of lipid research48(12)2736-50(2007 Dec)
Watkins PA,Maiguel D,Jia Z,Pevsner J

Expression of acetoacetyl-CoA synthetase, a novel cytosolic ketone body-utilizing enzyme, in human brain.
Biochemical pharmacology65(6)989-94(2003 Mar)
Ohgami M,Takahashi N,Yamasaki M,Fukui T

Caenorhabditis elegans SUR-5, a novel but conserved protein, negatively regulates LET-60 Ras activity during vulval induction.
Molecular and cellular biology18(8)4556-64(1998 Aug)
Gu T,Orita S,Han M

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AACS cDNA ORF clone, Homo sapiens(human)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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