SNRPA cDNA ORF clone, Homo sapiens (human)
| Gene Symbol | SNRPA |
| Entrez Gene ID | 6626 |
| Full Name | small nuclear ribonucleoprotein polypeptide A |
| Synonyms | Mud1, U1-A, U1A |
| Gene Type | protein-coding |
| Organism | Homo sapiens (human) |
| Genome | |
| Summary | The protein encoded by this gene associates with stem loop II of the U1 small nuclear ribonucleoprotein, which binds the 5' splice site of precursor mRNAs and is required for splicing. The encoded protein autoregulates itself by polyadenylation inhibition of its own pre-mRNA via dimerization and has been implicated in the coupling of splicing and polyadenylation. [provided by RefSeq, Oct 2010]. lac of sum |
| Disorder MIM: |
mRNA and Protein(s)
| mRNA | Protein | Name |
|---|---|---|
| NM_004596 | NP_004587 | U1 small nuclear ribonucleoprotein A |
| Homo sapiens (human) | SNRPA | NP_004587.1 |
| Pan troglodytes (chimpanzee) | SNRPA | XP_512674.2 |
| Macaca mulatta (Rhesus monkey) | SNRPA | XP_001098504.1 |
| Canis lupus familiaris (dog) | SNRPA | XP_533663.2 |
| Bos taurus (cattle) | SNRPA | NP_001039504.1 |
| Rattus norvegicus (Norway rat) | Snrpa | NP_001008304.1 |
| Danio rerio (zebrafish) | snrpa | NP_955965.1 |
| Drosophila melanogaster (fruit fly) | snf | NP_511045.1 |
| Caenorhabditis elegans | rnp-2 | NP_500504.1 |
| Arabidopsis thaliana (thale cress) | U1A | NP_182280.1 |
| Xenopus (Silurana) tropicalis (western clawed frog) | snrpa | NP_001016784.1 |
Related articles in PubMed
- Deubiquitinase FAM/USP9X interacts with the E3 ubiquitin ligase SMURF1 protein and protects it from ligase activity-dependent self-degradation. Xie Y, et al. J Biol Chem, 2013 Feb 1. PMID 23184937.
- Cleavage and polyadenylation specificity factor 1 (CPSF1) regulates alternative splicing of interleukin 7 receptor (IL7R) exon 6. Evsyukova I, et al. RNA, 2013 Jan. PMID 23151878.
- A census of human soluble protein complexes. Havugimana PC, et al. Cell, 2012 Aug 31. PMID 22939629.
- A high-throughput approach for measuring temporal changes in the interactome. Kristensen AR, et al. Nat Methods, 2012 Sep. PMID 22863883.
- Dynamic protein-protein interaction wiring of the human spliceosome. Hegele A, et al. Mol Cell, 2012 Feb 24. PMID 22365833.
See all (57) citations in PubMed
See citations in PubMed for homologs of this gene provided by HomoloGene
GeneRIFs: Gene References Into Functions What's a GeneRIF?
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An analysis of the kinetic data suggests three phases of U1A-SL2 RNA complex dissociation with time scales of approximately 100 mus, approximately 50 ms, and approximately 2 s. We propose that the first step of dissociation is a fast rearrangement of the complex to form a loosely bound complex.
Title: Multistep kinetics of the U1A-SL2 RNA complex dissociation.
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structural analysis of U1A protein-stem loop 2 RNA recognition
Title: U1A protein-stem loop 2 RNA recognition: prediction of structural differences from protein mutations.
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study shows that the yellow fever virus (YFV) NS5 protein is able to interact with U1A, a protein involved in splicing and polyadenylation; a region between amino acids 368 and 448 was identified as the site of interaction of the NS5 protein with U1A
Title: The small nuclear ribonucleoprotein U1A interacts with NS5 from yellow fever virus.
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Functional U1A site in a cellular gene and of a single gene containing two dissimilar elements that inhibit nuclear polyadenylation.
Title: A bipartite U1 site represses U1A expression by synergizing with PIE to inhibit nuclear polyadenylation.
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Identification of PSF, p54(nrb), PTB, and U1A as proteins specifically bound to the COX-2 polyadenylation signal upstream sequence elements .
Title: Specific trans-acting proteins interact with auxiliary RNA polyadenylation elements in the COX-2 3'-UTR.
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13)C NMR relaxation studies of base and ribose dynamics for the RNA internal loop target of human U1A protein located within the 3'-untranslated region (3'-UTR) of the mRNA coding for U1A itself
Title: 13C NMR relaxation studies of RNA base and ribose nuclei reveal a complex pattern of motions in the RNA binding site for human U1A protein.
The following SNRPA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SNRPA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
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| CloneID | OHu15951  |
|
| Clone ID Related Accession (Same CDS sequence) | NM_004596 | |
| Accession Version | NM_004596.4 | Documents for ORF clone product in dufault vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 849bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | COA |
| ORF Insert Method | CloneEZ® Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 15-MAR-2015 | |
| Organism | Homo sapiens (human) | |
| Product | U1 small nuclear ribonucleoprotein A | |
| Comment | REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN286572.1, BC000405.2, AC020945.6 and BQ002918.1. On Apr 28, 2010 this sequence version replaced gi:39725715. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC000405.2, X06347.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGCAGTTC CCGAGACCCG CCCTAACCAC ACTATTTATA TCAACAACCT CAATGAGAAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_004587.1 |
| CDS | 556..1404 |
| Misc Feature(1) | 508..510(+) |
| Misc Feature(2) | 559..561(+) |
| Misc Feature(3) | 574..840(+) |
| Misc Feature(4) | 586..807(+) |
| Misc Feature(5) | 592..831(+) |
| Misc Feature(6) | 733..735(+) |
| Misc Feature(7) | 946..948(+) |
| Misc Feature(8) | 1162..1401(+) |
| Misc Feature(9) | 1183..1362(+) |
| Exon (1) | 1..628 Gene:SNRPA Gene Synonym: |
| Exon (2) | 629..801 Gene:SNRPA Gene Synonym: |
| Exon (3) | 802..981 Gene:SNRPA Gene Synonym: |
| Exon (4) | 982..1155 Gene:SNRPA Gene Synonym: |
| Exon (5) | 1156..1244 Gene:SNRPA Gene Synonym: |
| Exon (6) | 1245..1629 Gene:SNRPA Gene Synonym: |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_004596 |
ORF Insert Sequence:
1 | ATGGCAGTTC CCGAGACCCG CCCTAACCAC ACTATTTATA TCAACAACCT CAATGAGAAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
 |
U1A regulates 3' processing of the survival motor neuron mRNA |
|
U1A protein-stem loop 2 RNA recognition: prediction of structural differences from protein mutations |
|
The small nuclear ribonucleoprotein U1A interacts with NS5 from yellow fever virus |
|
Multistep kinetics of the U1A-SL2 RNA complex dissociation |
|
Identification of molecular contacts between the U1 A small nuclear ribonucleoprotein and U1 RNA |
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Structure, chromosomal localization and evolutionary conservation of the gene encoding human U1 snRNP-specific A protein |
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RNA-binding domain of the A protein component of the U1 small nuclear ribonucleoprotein analyzed by NMR spectroscopy is structurally similar to ribosomal proteins |
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Quantitative determination that one of two potential RNA-binding domains of the A protein component of the U1 small nuclear ribonucleoprotein complex binds with high affinity to stem-loop II of U1 RNA |
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Assignment of seven genes to distinct intervals on the midportion of human chromosome 19q surrounding the myotonic dystrophy gene region |
|
cDNA cloning of the human U1 snRNA-associated A protein: extensive homology between U1 and U2 snRNP-specific proteins |
