WNT8B cDNA ORF clone, Homo sapiens(human)
| Gene Symbol | WNT8B |
| Entrez Gene ID | 7479 |
| Full Name | Wnt family member 8B |
| General protein information |
|
| Gene Type | protein-coding |
| Organism | Homo sapiens(human) |
| Genome | |
| Summary | The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 95%, 86% and 71% amino acid identity to the mouse, zebrafish and Xenopus Wnt8B proteins, respectively. The expression patterns of the human and mouse genes appear identical and are restricted to the developing brain. The chromosomal location of this gene to 10q24 suggests it as a candidate gene for partial epilepsy. [provided by RefSeq, Jul 2008]. |
| Disorder MIM: |
mRNA and Protein(s)
| mRNA | Protein | Name |
|---|---|---|
| NM_003393.3 | NP_003384.2 | protein Wnt-8b precursor |
| NM_003393.4 | NP_003384.2 | protein Wnt-8b precursor |
| Rattus norvegicus (Norway rat) | Wnt8b | NP_001099829.1 |
| Gallus gallus (chicken) | WNT8B | XP_426508.3 |
| Homo sapiens (human) | WNT8B | NP_003384.2 |
| Canis lupus familiaris (dog) | WNT8B | XP_005637677.1 |
| Pan troglodytes (chimpanzee) | WNT8B | XP_521584.2 |
| WNT8B | XP_001108075.1 | |
| Mus musculus (house mouse) | Wnt8b | NP_035850.2 |
| Danio rerio (zebrafish) | wnt8b | NP_571034.1 |
| Xenopus tropicalis (tropical clawed frog) | wnt8b | XP_004915998.1 |
| Bos taurus (cattle) | WNT8B | XP_002698537.2 |
Related articles in PubMed
Mapping of Wnt-Frizzled interactions by multiplex CRISPR targeting of receptor gene families.
Voloshanenko O, Gmach P, Winter J, Kranz D, Boutros M
FASEB journal : official publication of the Federation of American Societies for Experimental Biology31(11)4832-4844(2017 Nov)
Genome-wide association study identifies novel loci associated with concentrations of four plasma phospholipid fatty acids in the de novo lipogenesis pathway: results from the Cohorts for Heart and Aging Research in Genomic Epidemiology (CHARGE) consortium.
Wu JH, Lemaitre RN, Manichaikul A, Guan W, Tanaka T, Foy M, Kabagambe EK, Djousse L, Siscovick D, Fretts AM, Johnson C, King IB, Psaty BM, McKnight B, Rich SS, Chen YD, Nettleton JA, Tang W, Bandinelli S, Jacobs DR Jr, Browning BL, Laurie CC, Gu X, Tsai MY, Steffen LM, Ferrucci L, Fornage M, Mozaffarian D
Circulation. Cardiovascular genetics6(2)171-83(2013 Apr)
[Mutation and expression of WNT8b gene and SHH gene in Hirschsprung disease].
Gao H, Zhang ZB, Jiang ZJ, Wang DJ, Huang Y, Wang WL
Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery13(10)758-61(2010 Oct)
Conserved POU/OCT- and GATA-binding sites in 5'-flanking promoter region of mammalian WNT8B orthologs.
Katoh M, Katoh M
International journal of oncology30(5)1273-7(2007 May)
A scan of chromosome 10 identifies a novel locus showing strong association with late-onset Alzheimer disease.
Grupe A, Li Y, Rowland C, Nowotny P, Hinrichs AL, Smemo S, Kauwe JS, Maxwell TJ, Cherny S, Doil L, Tacey K, van Luchene R, Myers A, Wavrant-De Vri?ze F, Kaleem M, Hollingworth P, Jehu L, Foy C, Archer N, Hamilton G, Holmans P, Morris CM, Catanese J, Sninsky J, White TJ, Powell J, Hardy J, O'Donovan M, Lovestone S, Jones L, Morris JC, Thal L, Owen M, Williams J, Goate A
American journal of human genetics78(1)78-88(2006 Jan)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
WNT8b and SHH mutations and abnormal expressions are present in the peripheral blood of children with sporadic Hirschsprung disease.
Title: [Mutation and expression of WNT8b gene and SHH gene in Hirschsprung disease].
WNT8B expression in hepatocyte progenitors derived from human ES cells is due to POU5F1 (OCT3/OCT4) and GATA3, and WNT8B expression in diffuse-type gastric cancer is due to POU5F1 and GATA6
Title: Conserved POU/OCT- and GATA-binding sites in 5'-flanking promoter region of mammalian WNT8B orthologs.
Observational study of gene-disease association. (HuGE Navigator)
Title: A scan of chromosome 10 identifies a novel locus showing strong association with late-onset Alzheimer disease.
Expression and regulation of WNT8B mRNA in human tumor cell lines
Title: Expression and regulation of WNT8A and WNT8B mRNAs in human tumor cell lines: up-regulation of WNT8B mRNA by beta-estradiol in MCF-7 cells, and down-regulation of WNT8A and WNT8B mRNAs by retinoic acid in NT2 cells.
significantly up-regulated in gastric cancer cell lines KATO-III (signet-ring cell carcinoma) and MKN45 (poorly differentiated adenocarcinoma), and also in 5 out of 10 cases of primary gastric cancer
Title: Up-regulation of WNT8B mRNA in human gastric cancer.
The following WNT8B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WNT8B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OHu17417 | |
| Clone ID Related Accession (Same CDS sequence) | NM_003393.3 , NM_003393.4 | |
| Accession Version | NM_003393.3 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1056bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-02-16 | |
| Organism | Homo sapiens(human) | |
| Product | protein Wnt-8b precursor | |
| Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN276452.1 and AB073637.1. On Jul 26, 2006 this sequence version replaced NM_003393.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB073637.1, Y11094.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2148874 [ECO:0000350] ##Evidence-Data-END## | |
1 | ATGTTTCTTT CAAAGCCTTC TGTGTACATC TGTCTTTTCA CCTGTGTCCT CCAACTCAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_003384.2 |
| CDS | 115..1170 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_003393.3 |
ORF Insert Sequence:
1 | ATGTTTCTTT CAAAGCCTTC TGTGTACATC TGTCTTTTCA CCTGTGTCCT CCAACTCAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu17417 | |
| Clone ID Related Accession (Same CDS sequence) | NM_003393.3 , NM_003393.4 | |
| Accession Version | NM_003393.4 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1056bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-10-24 | |
| Organism | Homo sapiens(human) | |
| Product | protein Wnt-8b precursor | |
| Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL359759.19, CN276452.1, AB073637.1 and AL133352.12. On May 17, 2019 this sequence version replaced NM_003393.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y11094.1, GQ891471.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2148874 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000343737.6/ ENSP00000340677.5 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## | |
1 | ATGTTTCTTT CAAAGCCTTC TGTGTACATC TGTCTTTTCA CCTGTGTCCT CCAACTCAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_003384.2 |
| CDS | 161..1216 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_003393.4 |
ORF Insert Sequence:
1 | ATGTTTCTTT CAAAGCCTTC TGTGTACATC TGTCTTTTCA CCTGTGTCCT CCAACTCAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
 |
Mapping of Wnt-Frizzled interactions by multiplex CRISPR targeting of receptor gene families. |
|
Genome-wide association study identifies novel loci associated with concentrations of four plasma phospholipid fatty acids in the de novo lipogenesis pathway: results from the Cohorts for Heart and Aging Research in Genomic Epidemiology (CHARGE) consortium. |
|
[Mutation and expression of WNT8b gene and SHH gene in Hirschsprung disease]. |
|
Conserved POU/OCT- and GATA-binding sites in 5'-flanking promoter region of mammalian WNT8B orthologs. |
|
A scan of chromosome 10 identifies a novel locus showing strong association with late-onset Alzheimer disease. |