NIFK cDNA ORF clone, Homo sapiens(human)
| Gene Symbol | NIFK |
| Entrez Gene ID | 84365 |
| Full Name | nucleolar protein interacting with the FHA domain of MKI67 |
| Synonyms | MKI67IP,Nopp34 |
| General protein information |
|
| Gene Type | protein-coding |
| Organism | Homo sapiens(human) |
| Genome | |
| Summary | This gene encodes a protein that interacts with the forkhead-associated domain of the Ki-67 antigen. The encoded protein may bind RNA and may play a role in mitosis and cell cycle progression. Multiple pseudogenes exist on chromosomes 5, 10, 12, 15, and 19.[provided by RefSeq, Jan 2009]. |
| Disorder MIM: |
mRNA and Protein(s)
| mRNA | Protein | Name |
|---|---|---|
| NM_032390.5 | NP_115766.3 | MKI67 FHA domain-interacting nucleolar phosphoprotein |
| NM_032390.4 | NP_115766.3 | MKI67 FHA domain-interacting nucleolar phosphoprotein |
| Canis lupus familiaris (dog) | MKI67IP | XP_003639749.1 |
| Mus musculus (house mouse) | Nifk | NP_080748.3 |
| Danio rerio (zebrafish) | nifk | NP_775395.1 |
| MKI67IP | XP_002799464.1 | |
| Oryza sativa (rice) | Os08g0412200 | NP_001061793.1 |
| Pan troglodytes (chimpanzee) | MKI67IP | XP_515769.3 |
| Rattus norvegicus (Norway rat) | Nifk | NP_631925.2 |
| Arabidopsis thaliana (thale cress) | AT5G04600 | NP_196080.1 |
| Homo sapiens (human) | NIFK | NP_115766.3 |
| Xenopus tropicalis (tropical clawed frog) | nifk | NP_001005639.1 |
| Bos taurus (cattle) | NIFK | NP_001029526.1 |
| Gallus gallus (chicken) | MKI67IP | XP_422088.1 |
Related articles in PubMed
The cell proliferation antigen Ki-67 organises heterochromatin.
Sobecki M, Mrouj K, Camasses A, Parisis N, Nicolas E, Ll?res D, Gerbe F, Prieto S, Krasinska L, David A, Eguren M, Birling MC, Urbach S, Hem S, D?jardin J, Malumbres M, Jay P, Dulic V, Lafontaine DLj, Feil R, Fisher D
eLife5e13722(2016 Mar)
The nucleolar protein NIFK promotes cancer progression via CK1?/?-catenin in metastasis and Ki-67-dependent cell proliferation.
Lin TC, Su CY, Wu PY, Lai TC, Pan WA, Jan YH, Chang YC, Yeh CT, Chen CL, Ger LP, Chang HT, Yang CJ, Huang MS, Liu YP, Lin YF, Shyy JY, Tsai MD, Hsiao M
eLife5(2016 Mar)
The RNA recognition motif of NIFK is required for rRNA maturation during cell cycle progression.
Pan WA, Tsai HY, Wang SC, Hsiao M, Wu PY, Tsai MD
RNA biology12(3)255-67(2015)
Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)
The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
NIFK is required for lung cancer progression via the RUNX1-dependent CK1alpha repression, which activates TCF4/beta-catenin signaling in metastasis and the Ki-67-dependent regulation in cell proliferation.
Title: The nucleolar protein NIFK promotes cancer progression via CK1?/?-catenin in metastasis and Ki-67-dependent cell proliferation.
The RNA recognition motif of NIFK is required for rRNA maturation during cell cycle progression.
Title: The RNA recognition motif of NIFK is required for rRNA maturation during cell cycle progression.
Potential encounter complexes of the Ki67FHA receptor and hNIFK peptide are misregistered states of the beta-sheet.
Title: Direct observations of shifts in the ?-sheet register of a protein-peptide complex using explicit solvent simulations.
Better basal gene expression was observed by co-cultured respiratory epithelial cells compared to dispase dissociated cells.
Title: Human nasal turbinates as a viable source of respiratory epithelial cells using co-culture system versus dispase-dissociation technique.
nmr analysis of the solution structure of the FHA domain of human Ki67 and mapping of the binding surface for NIFK binding
Title: Structure of human Ki67 FHA domain and its binding to a phosphoprotein fragment from hNIFK reveal unique recognition sites and new views to the structural basis of FHA domain functions.
The following NIFK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NIFK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OHu04995 |
|
| Clone ID Related Accession (Same CDS sequence) | NM_032390.5 , NM_032390.4 | |
| Accession Version | NM_032390.5 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 882bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-29 | |
| Organism | Homo sapiens(human) | |
| Product | MKI67 FHA domain-interacting nucleolar phosphoprotein | |
| Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC022990.1, BC024238.2 and BE671589.1. On Nov 22, 2018 this sequence version replaced NM_032390.4. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.257496.1, SRR1660803.182395.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000285814.9/ ENSP00000285814.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length. | |
1 | ATGGCGACTT TTTCTGGCCC GGCTGGGCCA ATCCTGTCGC TTAATCCGCA GGAAGATGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_115766.3 |
| CDS | 26..907 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_032390.5 |
ORF Insert Sequence:
1 | ATGGCGACTT TTTCTGGCCC GGCTGGGCCA ATCCTGTCGC TTAATCCGCA GGAAGATGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu04995 |
|
| Clone ID Related Accession (Same CDS sequence) | NM_032390.5 , NM_032390.4 | |
| Accession Version | NM_032390.4 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 882bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-10-01 | |
| Organism | Homo sapiens(human) | |
| Product | MKI67 FHA domain-interacting nucleolar phosphoprotein | |
| Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA570244.1, BC022990.1, BC024238.2 and BE671589.1. On Jan 31, 2009 this sequence version replaced NM_032390.3. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.44628.1, SRR1660803.182395.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGCGACTT TTTCTGGCCC GGCTGGGCCA ATCCTGTCGC TTAATCCGCA GGAAGATGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_115766.3 |
| CDS | 78..959 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_032390.4 |
ORF Insert Sequence:
1 | ATGGCGACTT TTTCTGGCCC GGCTGGGCCA ATCCTGTCGC TTAATCCGCA GGAAGATGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
 |
The cell proliferation antigen Ki-67 organises heterochromatin. |
|
The nucleolar protein NIFK promotes cancer progression via CK1?/?-catenin in metastasis and Ki-67-dependent cell proliferation. |
|
The RNA recognition motif of NIFK is required for rRNA maturation during cell cycle progression. |
|
Insights into RNA biology from an atlas of mammalian mRNA-binding proteins. |
|
The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts. |