NIFK cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NIFK
Entrez Gene ID	84365
Full Name	nucleolar protein interacting with the FHA domain of MKI67
Synonyms	MKI67IP,Nopp34
General protein information	Preferred Names nucleolar protein interacting with the FHA domain of MKI67 Names MKI67 FHA domain-interacting nucleolar phosphoprotein hNIFK nucleolar phosphoprotein Nopp34 nucleolar protein interacting with the FHA domain of pKi-67
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q14.3
Summary	This gene encodes a protein that interacts with the forkhead-associated domain of the Ki-67 antigen. The encoded protein may bind RNA and may play a role in mitosis and cell cycle progression. Multiple pseudogenes exist on chromosomes 5, 10, 12, 15, and 19.[provided by RefSeq, Jan 2009].
Disorder MIM:	611970

mRNA and Protein(s)

mRNA	Protein	Name
NM_032390.5	NP_115766.3	MKI67 FHA domain-interacting nucleolar phosphoprotein
NM_032390.4	NP_115766.3	MKI67 FHA domain-interacting nucleolar phosphoprotein

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MKI67IP	XP_003639749.1
Mus musculus (house mouse)	Nifk	NP_080748.3
Danio rerio (zebrafish)	nifk	NP_775395.1
	MKI67IP	XP_002799464.1
Oryza sativa (rice)	Os08g0412200	NP_001061793.1
Pan troglodytes (chimpanzee)	MKI67IP	XP_515769.3
Rattus norvegicus (Norway rat)	Nifk	NP_631925.2
Arabidopsis thaliana (thale cress)	AT5G04600	NP_196080.1
Homo sapiens (human)	NIFK	NP_115766.3
Xenopus tropicalis (tropical clawed frog)	nifk	NP_001005639.1
Bos taurus (cattle)	NIFK	NP_001029526.1
Gallus gallus (chicken)	MKI67IP	XP_422088.1

Gene Ontology
Bibliography

Related articles in PubMed

The cell proliferation antigen Ki-67 organises heterochromatin.
Sobecki M, Mrouj K, Camasses A, Parisis N, Nicolas E, Ll?res D, Gerbe F, Prieto S, Krasinska L, David A, Eguren M, Birling MC, Urbach S, Hem S, D?jardin J, Malumbres M, Jay P, Dulic V, Lafontaine DLj, Feil R, Fisher D
eLife5e13722(2016 Mar)

The nucleolar protein NIFK promotes cancer progression via CK1?/?-catenin in metastasis and Ki-67-dependent cell proliferation.
Lin TC, Su CY, Wu PY, Lai TC, Pan WA, Jan YH, Chang YC, Yeh CT, Chen CL, Ger LP, Chang HT, Yang CJ, Huang MS, Liu YP, Lin YF, Shyy JY, Tsai MD, Hsiao M
eLife5(2016 Mar)

The RNA recognition motif of NIFK is required for rRNA maturation during cell cycle progression.
Pan WA, Tsai HY, Wang SC, Hsiao M, Wu PY, Tsai MD
RNA biology12(3)255-67(2015)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NIFK is required for lung cancer progression via the RUNX1-dependent CK1alpha repression, which activates TCF4/beta-catenin signaling in metastasis and the Ki-67-dependent regulation in cell proliferation.
Title: The nucleolar protein NIFK promotes cancer progression via CK1?/?-catenin in metastasis and Ki-67-dependent cell proliferation.

The RNA recognition motif of NIFK is required for rRNA maturation during cell cycle progression.
Title: The RNA recognition motif of NIFK is required for rRNA maturation during cell cycle progression.

Potential encounter complexes of the Ki67FHA receptor and hNIFK peptide are misregistered states of the beta-sheet.
Title: Direct observations of shifts in the ?-sheet register of a protein-peptide complex using explicit solvent simulations.

Better basal gene expression was observed by co-cultured respiratory epithelial cells compared to dispase dissociated cells.
Title: Human nasal turbinates as a viable source of respiratory epithelial cells using co-culture system versus dispase-dissociation technique.

nmr analysis of the solution structure of the FHA domain of human Ki67 and mapping of the binding surface for NIFK binding
Title: Structure of human Ki67 FHA domain and its binding to a phosphoprotein fragment from hNIFK reveal unique recognition sites and new views to the structural basis of FHA domain functions.

The following NIFK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NIFK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu04995	NM_032390.5 Latest version!	Homo sapiens nucleolar protein interacting with the FHA domain of MKI67 (NIFK), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
View Old Accession Versions >>
OHu04995	NM_032390.4	Homo sapiens nucleolar protein interacting with the FHA domain of MKI67 (NIFK), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NIFK ( NM_032390.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_115766.3 Homo sapiens nucleolar protein interacting with the FHA domain of MKI67 (NIFK), mRNA.

Price & Availability↑

CloneID	OHu04995
Clone ID Related Accession (Same CDS sequence)	NM_032390.5 , NM_032390.4
Accession Version	NM_032390.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	MKI67 FHA domain-interacting nucleolar phosphoprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC022990.1, BC024238.2 and BE671589.1. On Nov 22, 2018 this sequence version replaced NM_032390.4. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.257496.1, SRR1660803.182395.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000285814.9/ ENSP00000285814.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGACTT TTTCTGGCCC GGCTGGGCCA ATCCTGTCGC TTAATCCGCA GGAAGATGTC 
GAGTTTCAAA AGGAGGTGGC GCAGGTTCGC AAGCGCATAA CCCAGCGAAA AAAACAAGAA 
CAACTTACTC CTGGAGTAGT CTATGTGCGC CACCTACCTA ACCTACTTGA CGAAACCCAG 
ATCTTTTCAT ATTTCTCCCA GTTTGGCACT GTGACACGGT TCAGGCTGTC CAGAAGTAAA 
AGGACTGGAA ATAGCAAAGG CTATGCATTT GTGGAGTTTG AGTCTGAGGA TGTTGCCAAA 
ATAGTTGCTG AAACAATGAA CAACTACCTG TTTGGTGAAA GACTCTTGGA GTGTCATTTT 
ATGCCACCTG AAAAAGTACA TAAAGAACTC TTTAAAGACT GGAATATTCC ATTTAAGCAG 
CCATCATATC CATCAGTGAA ACGGTATAAT CGGAATCGGA CACTAACACA AAAGCTACGG 
ATGGAGGAGC GATTTAAAAA GAAAGAAAGA TTACTCAGGA AGAAATTAGC TAAAAAAGGA 
ATTGACTATG ATTTTCCTTC TTTGATTTTA CAGAAAACGG AAAGTATTTC AAAAACTAAT 
CGTCAGACGT CTACAAAAGG CCAGGTTTTA CGTAAGAAGA AGAAAAAAGT TTCAGGTACT 
CTTGACACTC CTGAGAAGAC TGTGGATAGC CAGGGCCCCA CACCAGTTTG TACACCAACA 
TTTTTGGAGA GGCGAAAATC TCAAGTGGCT GAACTGAATG ATGATGATAA AGATGATGAA 
ATAGTTTTCA AACAGCCCAT ATCCTGTGTA AAAGAAGAAA TACAAGAGAC TCAAACACCT 
ACACATTCAC GGAAAAAAAG ACGAAGAAGC AGCAATCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_115766.3
CDS	26..907
Translation	MATFSGPAGPILSLNPQEDVEFQKEVAQVRKRITQRKKQEQLTPGVVYVRHLPNLLDETQIFSYFSQFGTVTRFRLSRSKRTGNSKGYAFVEFESEDVAKIVAETMNNYLFGERLLECHFMPPEKVHKELFKDWNIPFKQPSYPSVKRYNRNRTLTQKLRMEERFKKKERLLRKKLAKKGIDYDFPSLILQKTESISKTNRQTSTKGQVLRKKKKKVSGTLDTPEKTVDSQGPTPVCTPTFLERRKSQVAELNDDDKDDEIVFKQPISCVKEEIQETQTPTHSRKKRRRSSNQ

Target ORF information:

RefSeq Version	NM_032390.5
Organism	Homo sapiens(human)
Definition	Homo sapiens nucleolar protein interacting with the FHA domain of MKI67 (NIFK), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032390.5

ORF Insert Sequence:

ATGGCGACTT TTTCTGGCCC GGCTGGGCCA ATCCTGTCGC TTAATCCGCA GGAAGATGTC 
GAGTTTCAAA AGGAGGTGGC GCAGGTTCGC AAGCGCATAA CCCAGCGAAA AAAACAAGAA 
CAACTTACTC CTGGAGTAGT CTATGTGCGC CACCTACCTA ACCTACTTGA CGAAACCCAG 
ATCTTTTCAT ATTTCTCCCA GTTTGGCACT GTGACACGGT TCAGGCTGTC CAGAAGTAAA 
AGGACTGGAA ATAGCAAAGG CTATGCATTT GTGGAGTTTG AGTCTGAGGA TGTTGCCAAA 
ATAGTTGCTG AAACAATGAA CAACTACCTG TTTGGTGAAA GACTCTTGGA GTGTCATTTT 
ATGCCACCTG AAAAAGTACA TAAAGAACTC TTTAAAGACT GGAATATTCC ATTTAAGCAG 
CCATCATATC CATCAGTGAA ACGGTATAAT CGGAATCGGA CACTAACACA AAAGCTACGG 
ATGGAGGAGC GATTTAAAAA GAAAGAAAGA TTACTCAGGA AGAAATTAGC TAAAAAAGGA 
ATTGACTATG ATTTTCCTTC TTTGATTTTA CAGAAAACGG AAAGTATTTC AAAAACTAAT 
CGTCAGACGT CTACAAAAGG CCAGGTTTTA CGTAAGAAGA AGAAAAAAGT TTCAGGTACT 
CTTGACACTC CTGAGAAGAC TGTGGATAGC CAGGGCCCCA CACCAGTTTG TACACCAACA 
TTTTTGGAGA GGCGAAAATC TCAAGTGGCT GAACTGAATG ATGATGATAA AGATGATGAA 
ATAGTTTTCA AACAGCCCAT ATCCTGTGTA AAAGAAGAAA TACAAGAGAC TCAAACACCT 
ACACATTCAC GGAAAAAAAG ACGAAGAAGC AGCAATCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIFK ( NM_032390.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_115766.3 Homo sapiens nucleolar protein interacting with the FHA domain of MKI67 (NIFK), mRNA.

Price & Availability↑

CloneID	OHu04995
Clone ID Related Accession (Same CDS sequence)	NM_032390.5 , NM_032390.4
Accession Version	NM_032390.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	MKI67 FHA domain-interacting nucleolar phosphoprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA570244.1, BC022990.1, BC024238.2 and BE671589.1. On Jan 31, 2009 this sequence version replaced NM_032390.3. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.44628.1, SRR1660803.182395.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGACTT TTTCTGGCCC GGCTGGGCCA ATCCTGTCGC TTAATCCGCA GGAAGATGTC 
GAGTTTCAAA AGGAGGTGGC GCAGGTTCGC AAGCGCATAA CCCAGCGAAA AAAACAAGAA 
CAACTTACTC CTGGAGTAGT CTATGTGCGC CACCTACCTA ACCTACTTGA CGAAACCCAG 
ATCTTTTCAT ATTTCTCCCA GTTTGGCACT GTGACACGGT TCAGGCTGTC CAGAAGTAAA 
AGGACTGGAA ATAGCAAAGG CTATGCATTT GTGGAGTTTG AGTCTGAGGA TGTTGCCAAA 
ATAGTTGCTG AAACAATGAA CAACTACCTG TTTGGTGAAA GACTCTTGGA GTGTCATTTT 
ATGCCACCTG AAAAAGTACA TAAAGAACTC TTTAAAGACT GGAATATTCC ATTTAAGCAG 
CCATCATATC CATCAGTGAA ACGGTATAAT CGGAATCGGA CACTAACACA AAAGCTACGG 
ATGGAGGAGC GATTTAAAAA GAAAGAAAGA TTACTCAGGA AGAAATTAGC TAAAAAAGGA 
ATTGACTATG ATTTTCCTTC TTTGATTTTA CAGAAAACGG AAAGTATTTC AAAAACTAAT 
CGTCAGACGT CTACAAAAGG CCAGGTTTTA CGTAAGAAGA AGAAAAAAGT TTCAGGTACT 
CTTGACACTC CTGAGAAGAC TGTGGATAGC CAGGGCCCCA CACCAGTTTG TACACCAACA 
TTTTTGGAGA GGCGAAAATC TCAAGTGGCT GAACTGAATG ATGATGATAA AGATGATGAA 
ATAGTTTTCA AACAGCCCAT ATCCTGTGTA AAAGAAGAAA TACAAGAGAC TCAAACACCT 
ACACATTCAC GGAAAAAAAG ACGAAGAAGC AGCAATCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_115766.3
CDS	78..959
Translation	MATFSGPAGPILSLNPQEDVEFQKEVAQVRKRITQRKKQEQLTPGVVYVRHLPNLLDETQIFSYFSQFGTVTRFRLSRSKRTGNSKGYAFVEFESEDVAKIVAETMNNYLFGERLLECHFMPPEKVHKELFKDWNIPFKQPSYPSVKRYNRNRTLTQKLRMEERFKKKERLLRKKLAKKGIDYDFPSLILQKTESISKTNRQTSTKGQVLRKKKKKVSGTLDTPEKTVDSQGPTPVCTPTFLERRKSQVAELNDDDKDDEIVFKQPISCVKEEIQETQTPTHSRKKRRRSSNQ

Target ORF information:

RefSeq Version	NM_032390.4
Organism	Homo sapiens(human)
Definition	Homo sapiens nucleolar protein interacting with the FHA domain of MKI67 (NIFK), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032390.4

ORF Insert Sequence:

ATGGCGACTT TTTCTGGCCC GGCTGGGCCA ATCCTGTCGC TTAATCCGCA GGAAGATGTC 
GAGTTTCAAA AGGAGGTGGC GCAGGTTCGC AAGCGCATAA CCCAGCGAAA AAAACAAGAA 
CAACTTACTC CTGGAGTAGT CTATGTGCGC CACCTACCTA ACCTACTTGA CGAAACCCAG 
ATCTTTTCAT ATTTCTCCCA GTTTGGCACT GTGACACGGT TCAGGCTGTC CAGAAGTAAA 
AGGACTGGAA ATAGCAAAGG CTATGCATTT GTGGAGTTTG AGTCTGAGGA TGTTGCCAAA 
ATAGTTGCTG AAACAATGAA CAACTACCTG TTTGGTGAAA GACTCTTGGA GTGTCATTTT 
ATGCCACCTG AAAAAGTACA TAAAGAACTC TTTAAAGACT GGAATATTCC ATTTAAGCAG 
CCATCATATC CATCAGTGAA ACGGTATAAT CGGAATCGGA CACTAACACA AAAGCTACGG 
ATGGAGGAGC GATTTAAAAA GAAAGAAAGA TTACTCAGGA AGAAATTAGC TAAAAAAGGA 
ATTGACTATG ATTTTCCTTC TTTGATTTTA CAGAAAACGG AAAGTATTTC AAAAACTAAT 
CGTCAGACGT CTACAAAAGG CCAGGTTTTA CGTAAGAAGA AGAAAAAAGT TTCAGGTACT 
CTTGACACTC CTGAGAAGAC TGTGGATAGC CAGGGCCCCA CACCAGTTTG TACACCAACA 
TTTTTGGAGA GGCGAAAATC TCAAGTGGCT GAACTGAATG ATGATGATAA AGATGATGAA 
ATAGTTTTCA AACAGCCCAT ATCCTGTGTA AAAGAAGAAA TACAAGAGAC TCAAACACCT 
ACACATTCAC GGAAAAAAAG ACGAAGAAGC AGCAATCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The cell proliferation antigen Ki-67 organises heterochromatin.
eLife5e13722(2016 Mar)
Sobecki M,Mrouj K,Camasses A,Parisis N,Nicolas E,Ll?res D,Gerbe F,Prieto S,Krasinska L,David A,Eguren M,Birling MC,Urbach S,Hem S,D?jardin J,Malumbres M,Jay P,Dulic V,Lafontaine DLj,Feil R,Fisher D

The nucleolar protein NIFK promotes cancer progression via CK1?/?-catenin in metastasis and Ki-67-dependent cell proliferation.
eLife5(2016 Mar)
Lin TC,Su CY,Wu PY,Lai TC,Pan WA,Jan YH,Chang YC,Yeh CT,Chen CL,Ger LP,Chang HT,Yang CJ,Huang MS,Liu YP,Lin YF,Shyy JY,Tsai MD,Hsiao M

The RNA recognition motif of NIFK is required for rRNA maturation during cell cycle progression.
RNA biology12(3)255-67(2015)
Pan WA,Tsai HY,Wang SC,Hsiao M,Wu PY,Tsai MD

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

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NIFK cDNA ORF clone, Homo sapiens(human)

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Related articles in PubMed

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ORF Insert Sequence:

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