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CCNA1 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol CCNA1
Entrez Gene ID 8900
Full Name cyclin A1
Synonyms CT146
General protein information
Preferred Names

cyclin A1

Names

cyclin-A1
testicular tissue protein Li 34

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

13

13q13.3

Summary The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. The cyclin encoded by this gene was shown to be expressed in testis and brain, as well as in several leukemic cell lines, and is thought to primarily function in the control of the germline meiotic cell cycle. This cyclin binds both CDK2 and CDC2 kinases, which give two distinct kinase activities, one appearing in S phase, the other in G2, and thus regulate separate functions in cell cycle. This cyclin was found to bind to important cell cycle regulators, such as Rb family proteins, transcription factor E2F-1, and the p21 family proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:

604036


mRNA and Protein(s)

mRNA Protein Name
NM_001111045.1 NP_001104515.1 cyclin-A1 isoform b
XM_011535296.2 XP_011533598.1 cyclin-A1 isoform X1
XM_011535294.1 XP_011533596.1 cyclin-A1 isoform X1
XM_011535297.1 XP_011533599.1 cyclin-A1 isoform X1
XM_011535296.1 XP_011533598.1 cyclin-A1 isoform X1
NM_001111046.1 NP_001104516.1 cyclin-A1 isoform c
NM_001111047.1 NP_001104517.1 cyclin-A1 isoform c
XM_011535294.2 XP_011533596.1 cyclin-A1 isoform X1
XM_011535295.2 XP_011533597.1 cyclin-A1 isoform X1
XM_011535295.1 XP_011533597.1 cyclin-A1 isoform X1
NM_003914.3 NP_003905.1 cyclin-A1 isoform a



Pan troglodytes (chimpanzee) CCNA1 NP_001233366.1
Bos taurus (cattle) CCNA1 XP_005213798.1
Arabidopsis thaliana (thale cress) CYCA3;4 NP_175156.1
Xenopus tropicalis (tropical clawed frog) ccna1 NP_001016239.1
Homo sapiens (human) CCNA1 NP_003905.1
Rattus norvegicus (Norway rat) Ccna1 NP_001011949.1
Gallus gallus (chicken) CCNA1 XP_417097.1
Arabidopsis thaliana (thale cress) CYCA3;2 NP_564499.3
Oryza sativa (rice) Os12g0581800 NP_001067137.1
Oryza sativa (rice) Os03g0607600 NP_001050640.2
Mus musculus (house mouse) Ccna1 NP_031654.2
Macaca mulatta (rhesus monkey) CCNA1 XP_001084161.1
Canis lupus familiaris (dog) CCNA1 XP_005635513.1
Danio rerio (zebrafish) ccna1 NP_997983.1
Arabidopsis thaliana (thale cress) CYCA3;3 NP_175155.1
Arabidopsis thaliana (thale cress) CYCA3;1 NP_199122.1


Related articles in PubMed

Human papillomavirus type 16 E7 oncoprotein mediates CCNA1 promoter methylation.
Chalertpet K, Pakdeechaidan W, Patel V, Mutirangura A, Yanatatsaneejit P
Cancer science106(10)1333-40(2015 Oct)

Correlation of CCNA1 promoter methylation with malignant tumors: a meta-analysis introduction.
Yang B, Miao S, Zhang LN, Sun HB, Xu ZN, Han CS
BioMed research international2015134027(2015)

DNA methylation status of key cell-cycle regulators such as CDKNA2/p16 and CCNA1 correlates with treatment response to doxorubicin and 5-fluorouracil in locally advanced breast tumors.
Klajic J, Busato F, Edvardsen H, Touleimat N, Fleischer T, Bukholm I, Børresen-Dale AL, Lønning PE, Tost J, Kristensen VN
Clinical cancer research : an official journal of the American Association for Cancer Research20(24)6357-66(2014 Dec)

Elucidating the roles of miR-372 in cell proliferation and apoptosis of nasopharyngeal carcinoma TW01 cells.
Tan JK, Tan EL, Gan SY
Experimental oncology36(3)170-3(2014 Sep)

CCNA1 promoter methylation: a potential marker for grading Papanicolaou smear cervical squamous intraepithelial lesions.
Chujan S, Kitkumthorn N, Siriangkul S, Mutirangura A
Asian Pacific journal of cancer prevention : APJCP15(18)7971-5(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Cyclin A1 functions as an oncogene that controls proliferative and survival activities in tumourigenesis and chemoresistance of ovarian cancer.
Title: Cyclin A1 expression and paclitaxel resistance in human ovarian cancer cells.

In the bone marrow, cyclin A1 and aromatase enhanced local bone marrow-releasing factors, including androgen receptor, estrogen and matrix metalloproteinase MMP9 and promoted the metastatic growth of prostate cancer cells
Title: Cyclin A1 and P450 Aromatase Promote Metastatic Homing and Growth of Stem-like Prostate Cancer Cells in the Bone Marrow.

The data showed that E7 induced CCNA1 methylation by forming a complex with Dnmt1 at the CCNA1 promoter, resulting in the subsequent reduction of expression in cancers.
Title: Human papillomavirus type 16 E7 oncoprotein mediates CCNA1 promoter methylation.

These data from results indicated a significant connection of CCNA1 methylation with poor progression in human malignant tumors among both Caucasian and Asian populations.
Title: Correlation of CCNA1 promoter methylation with malignant tumors: a meta-analysis introduction.

DNA methylation status of key cell-cycle regulators such as CDKNA2/p16 and CCNA1 correlates with treatment response to doxorubicin and 5-fluorouracil in locally advanced breast tumors.
Title: DNA methylation status of key cell-cycle regulators such as CDKNA2/p16 and CCNA1 correlates with treatment response to doxorubicin and 5-fluorouracil in locally advanced breast tumors.

The following CCNA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CCNA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu25352 NM_001111046.1
Latest version!
Homo sapiens cyclin A1 (CCNA1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $223.30
$319.00
OHu25352 NM_001111047.1
Latest version!
Homo sapiens cyclin A1 (CCNA1), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $223.30
$319.00
OHu25558 NM_001111045.1
Latest version!
Homo sapiens cyclin A1 (CCNA1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $265.30
$379.00
OHu25352 XM_011535294.2
Latest version!
Homo sapiens cyclin A1 (CCNA1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $223.30
$319.00
OHu25352 XM_011535296.2
Latest version!
Homo sapiens cyclin A1 (CCNA1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $223.30
$319.00
OHu25352 XM_011535297.1
Latest version!
Homo sapiens cyclin A1 (CCNA1), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $223.30
$319.00
OHu25352 XM_011535295.2
Latest version!
Homo sapiens cyclin A1 (CCNA1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $223.30
$319.00
OHu25389 NM_003914.3
Latest version!
Homo sapiens cyclin A1 (CCNA1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $265.30
$379.00
View Old Accession Versions >>
OHu25352 XM_011535296.1 Homo sapiens cyclin A1 (CCNA1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $223.30
$319.00
OHu25352 XM_011535294.1 Homo sapiens cyclin A1 (CCNA1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $223.30
$319.00
OHu25352 XM_011535295.1 Homo sapiens cyclin A1 (CCNA1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $223.30
$319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu25352
Clone ID Related Accession (Same CDS sequence) XM_011535296.2 , XM_011535294.1 , XM_011535297.1 , XM_011535296.1 , NM_001111046.1 , NM_001111047.1 , XM_011535294.2 , XM_011535295.2 , XM_011535295.1
Accession Version NM_001111046.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1266bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-02
Organism Homo sapiens(Human)
Product cyclin-A1 isoform c
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG723613.1, AL359767.13, CD171793.1, U66838.1 and AA553454.1. Transcript Variant: This variant (3) contains a distinct 5' UTR and lacks an in-frame portion of the 5' coding region, compared to variant 1. Transcript variants 3 and 4 encode isoform c, which has a shorter N-terminus when compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.72660.1, SRR1803614.65892.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145313 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
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181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGCACTGCA GCAACCCCAA GAGTGGAGTT GTGCTGGCTA CAGTGGCCCG AGGTCCCGAT 
GCTTGTCAGA TACTCACCAG AGCCCCGCTG GGCCAGGATC CCCCGCAGAG GACAGTGCTA
GGGCTGCTAA CTGCAAATGG GCAGTACAGG AGGACCTGTG GCCAGGGGAT CACAAGAATC
AGGTGTTATT CTGGATCAGA AAATGCCTTC CCTCCAGCTG GAAAGAAAGC ACTCCCTGAC
TGTGGGGTCC AAGAGCCCCC CAAGCAAGGG TTTGACATCT ACATGGATGA ACTAGAGCAG
GGGGACAGAG ACAGCTGCTC GGTCAGAGAG GGGATGGCAT TTGAGGATGT GTATGAAGTA
GACACCGGCA CACTCAAGTC AGACCTGCAC TTCCTGCTGG ATTTCAACAC AGTTTCCCCT
ATGCTGGTAG ATTCATCTCT CCTCTCCCAG TCTGAAGATA TATCCAGTCT TGGCACAGAT
GTGATAAATG TGACTGAATA TGCTGAAGAA ATTTATCAGT ACCTTAGGGA AGCTGAAATA
AGGCACAGAC CCAAAGCACA CTACATGAAG AAGCAGCCAG ACATCACGGA AGGCATGCGC
ACGATTCTGG TGGACTGGCT GGTGGAGGTT GGGGAAGAAT ATAAACTTCG AGCAGAGACC
CTGTATCTGG CTGTCAACTT CCTGGACAGG TTCCTTTCAT GTATGTCTGT TCTGAGAGGG
AAACTGCAGC TCGTAGGAAC AGCAGCTATG CTTTTGGCTT CGAAATATGA AGAGATATAT
CCTCCTGAAG TAGACGAGTT TGTCTATATC ACCGATGATA CATACACAAA ACGACAACTG
TTAAAAATGG AACACTTGCT TCTGAAAGTT CTAGCTTTTG ATCTGACAGT ACCAACCACC
AACCAGTTTC TCCTTCAGTA CTTGAGGCGA CAAGGAGTGT GCGTCAGGAC TGAGAACCTG
GCTAAGTACG TAGCAGAGCT GAGTCTACTT GAAGCAGATC CATTCTTGAA ATATCTTCCT
TCACTGATAG CTGCAGCAGC TTTTTGCCTG GCAAACTATA CTGTGAACAA GCACTTTTGG
CCAGAAACCC TTGCTGCATT TACAGGGTAT TCATTAAGTG AAATTGTGCC TTGCCTGAGT
GAGCTTCATA AAGCGTACCT TGATATACCC CATCGACCTC AGCAAGCAAT TAGGGAGAAG
TACAAGGCTT CAAAGTACCT GTGTGTGTCC CTCATGGAGC CACCTGCAGT TCTTCTTCTA
CAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001104516.1
CDS362..1627
Misc Feature(1)440..>664
Misc Feature(2)869..1249
Misc Feature(3)order(956..961,977..982,986..991,1055..1057,1085..1090)
Misc Feature(4)order(1112..1114,1124..1126,1148..1150,1199..1201,1208..1213)
Misc Feature(5)order(1247..1252,1268..1273,1277..1282,1346..1348,1379..1384)
Misc Feature(6)1253..1606
Misc Feature(7)order(1406..1408,1418..1420,1439..1441,1484..1486,1493..1498)
Exon (1)1..337
Exon (2)338..526
Exon (3)527..773
Exon (4)774..898
Exon (5)899..1122
Exon (6)1123..1327
Exon (7)1328..1441
Exon (8)1442..1575
Exon (9)1576..1844
Translation

Target ORF information:

RefSeq Version NM_001111046.1
Organism Homo sapiens(Human)
Definition Homo sapiens cyclin A1 (CCNA1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001111046.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGCACTGCA GCAACCCCAA GAGTGGAGTT GTGCTGGCTA CAGTGGCCCG AGGTCCCGAT 
GCTTGTCAGA TACTCACCAG AGCCCCGCTG GGCCAGGATC CCCCGCAGAG GACAGTGCTA
GGGCTGCTAA CTGCAAATGG GCAGTACAGG AGGACCTGTG GCCAGGGGAT CACAAGAATC
AGGTGTTATT CTGGATCAGA AAATGCCTTC CCTCCAGCTG GAAAGAAAGC ACTCCCTGAC
TGTGGGGTCC AAGAGCCCCC CAAGCAAGGG TTTGACATCT ACATGGATGA ACTAGAGCAG
GGGGACAGAG ACAGCTGCTC GGTCAGAGAG GGGATGGCAT TTGAGGATGT GTATGAAGTA
GACACCGGCA CACTCAAGTC AGACCTGCAC TTCCTGCTGG ATTTCAACAC AGTTTCCCCT
ATGCTGGTAG ATTCATCTCT CCTCTCCCAG TCTGAAGATA TATCCAGTCT TGGCACAGAT
GTGATAAATG TGACTGAATA TGCTGAAGAA ATTTATCAGT ACCTTAGGGA AGCTGAAATA
AGGCACAGAC CCAAAGCACA CTACATGAAG AAGCAGCCAG ACATCACGGA AGGCATGCGC
ACGATTCTGG TGGACTGGCT GGTGGAGGTT GGGGAAGAAT ATAAACTTCG AGCAGAGACC
CTGTATCTGG CTGTCAACTT CCTGGACAGG TTCCTTTCAT GTATGTCTGT TCTGAGAGGG
AAACTGCAGC TCGTAGGAAC AGCAGCTATG CTTTTGGCTT CGAAATATGA AGAGATATAT
CCTCCTGAAG TAGACGAGTT TGTCTATATC ACCGATGATA CATACACAAA ACGACAACTG
TTAAAAATGG AACACTTGCT TCTGAAAGTT CTAGCTTTTG ATCTGACAGT ACCAACCACC
AACCAGTTTC TCCTTCAGTA CTTGAGGCGA CAAGGAGTGT GCGTCAGGAC TGAGAACCTG
GCTAAGTACG TAGCAGAGCT GAGTCTACTT GAAGCAGATC CATTCTTGAA ATATCTTCCT
TCACTGATAG CTGCAGCAGC TTTTTGCCTG GCAAACTATA CTGTGAACAA GCACTTTTGG
CCAGAAACCC TTGCTGCATT TACAGGGTAT TCATTAAGTG AAATTGTGCC TTGCCTGAGT
GAGCTTCATA AAGCGTACCT TGATATACCC CATCGACCTC AGCAAGCAAT TAGGGAGAAG
TACAAGGCTT CAAAGTACCT GTGTGTGTCC CTCATGGAGC CACCTGCAGT TCTTCTTCTA
CAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25352
Clone ID Related Accession (Same CDS sequence) XM_011535296.2 , XM_011535294.1 , XM_011535297.1 , XM_011535296.1 , NM_001111046.1 , NM_001111047.1 , XM_011535294.2 , XM_011535295.2 , XM_011535295.1
Accession Version NM_001111047.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1266bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-02
Organism Homo sapiens(Human)
Product cyclin-A1 isoform c
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG723613.1, AL359767.13, DC395631.1, BC036346.1 and AA553454.1. Transcript Variant: This variant (4) contains a distinct 5' UTR, lacks an in-frame portion of the 5' coding region, and uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. Transcript variants 3 and 4 encode isoform c, which has a shorter N-terminus when compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.188555.1, SRR1660803.194880.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145313 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGCACTGCA GCAACCCCAA GAGTGGAGTT GTGCTGGCTA CAGTGGCCCG AGGTCCCGAT 
GCTTGTCAGA TACTCACCAG AGCCCCGCTG GGCCAGGATC CCCCGCAGAG GACAGTGCTA
GGGCTGCTAA CTGCAAATGG GCAGTACAGG AGGACCTGTG GCCAGGGGAT CACAAGAATC
AGGTGTTATT CTGGATCAGA AAATGCCTTC CCTCCAGCTG GAAAGAAAGC ACTCCCTGAC
TGTGGGGTCC AAGAGCCCCC CAAGCAAGGG TTTGACATCT ACATGGATGA ACTAGAGCAG
GGGGACAGAG ACAGCTGCTC GGTCAGAGAG GGGATGGCAT TTGAGGATGT GTATGAAGTA
GACACCGGCA CACTCAAGTC AGACCTGCAC TTCCTGCTGG ATTTCAACAC AGTTTCCCCT
ATGCTGGTAG ATTCATCTCT CCTCTCCCAG TCTGAAGATA TATCCAGTCT TGGCACAGAT
GTGATAAATG TGACTGAATA TGCTGAAGAA ATTTATCAGT ACCTTAGGGA AGCTGAAATA
AGGCACAGAC CCAAAGCACA CTACATGAAG AAGCAGCCAG ACATCACGGA AGGCATGCGC
ACGATTCTGG TGGACTGGCT GGTGGAGGTT GGGGAAGAAT ATAAACTTCG AGCAGAGACC
CTGTATCTGG CTGTCAACTT CCTGGACAGG TTCCTTTCAT GTATGTCTGT TCTGAGAGGG
AAACTGCAGC TCGTAGGAAC AGCAGCTATG CTTTTGGCTT CGAAATATGA AGAGATATAT
CCTCCTGAAG TAGACGAGTT TGTCTATATC ACCGATGATA CATACACAAA ACGACAACTG
TTAAAAATGG AACACTTGCT TCTGAAAGTT CTAGCTTTTG ATCTGACAGT ACCAACCACC
AACCAGTTTC TCCTTCAGTA CTTGAGGCGA CAAGGAGTGT GCGTCAGGAC TGAGAACCTG
GCTAAGTACG TAGCAGAGCT GAGTCTACTT GAAGCAGATC CATTCTTGAA ATATCTTCCT
TCACTGATAG CTGCAGCAGC TTTTTGCCTG GCAAACTATA CTGTGAACAA GCACTTTTGG
CCAGAAACCC TTGCTGCATT TACAGGGTAT TCATTAAGTG AAATTGTGCC TTGCCTGAGT
GAGCTTCATA AAGCGTACCT TGATATACCC CATCGACCTC AGCAAGCAAT TAGGGAGAAG
TACAAGGCTT CAAAGTACCT GTGTGTGTCC CTCATGGAGC CACCTGCAGT TCTTCTTCTA
CAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001104517.1
CDS359..1624
Misc Feature(1)437..>661
Misc Feature(2)866..1246
Misc Feature(3)order(953..958,974..979,983..988,1052..1054,1082..1087)
Misc Feature(4)order(1109..1111,1121..1123,1145..1147,1196..1198,1205..1210)
Misc Feature(5)order(1244..1249,1265..1270,1274..1279,1343..1345,1376..1381)
Misc Feature(6)1250..1603
Misc Feature(7)order(1403..1405,1415..1417,1436..1438,1481..1483,1490..1495)
Exon (1)1..337
Exon (2)338..523
Exon (3)524..770
Exon (4)771..895
Exon (5)896..1119
Exon (6)1120..1324
Exon (7)1325..1438
Exon (8)1439..1572
Exon (9)1573..1841
Translation

Target ORF information:

RefSeq Version NM_001111047.1
Organism Homo sapiens(Human)
Definition Homo sapiens cyclin A1 (CCNA1), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001111047.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGCACTGCA GCAACCCCAA GAGTGGAGTT GTGCTGGCTA CAGTGGCCCG AGGTCCCGAT 
GCTTGTCAGA TACTCACCAG AGCCCCGCTG GGCCAGGATC CCCCGCAGAG GACAGTGCTA
GGGCTGCTAA CTGCAAATGG GCAGTACAGG AGGACCTGTG GCCAGGGGAT CACAAGAATC
AGGTGTTATT CTGGATCAGA AAATGCCTTC CCTCCAGCTG GAAAGAAAGC ACTCCCTGAC
TGTGGGGTCC AAGAGCCCCC CAAGCAAGGG TTTGACATCT ACATGGATGA ACTAGAGCAG
GGGGACAGAG ACAGCTGCTC GGTCAGAGAG GGGATGGCAT TTGAGGATGT GTATGAAGTA
GACACCGGCA CACTCAAGTC AGACCTGCAC TTCCTGCTGG ATTTCAACAC AGTTTCCCCT
ATGCTGGTAG ATTCATCTCT CCTCTCCCAG TCTGAAGATA TATCCAGTCT TGGCACAGAT
GTGATAAATG TGACTGAATA TGCTGAAGAA ATTTATCAGT ACCTTAGGGA AGCTGAAATA
AGGCACAGAC CCAAAGCACA CTACATGAAG AAGCAGCCAG ACATCACGGA AGGCATGCGC
ACGATTCTGG TGGACTGGCT GGTGGAGGTT GGGGAAGAAT ATAAACTTCG AGCAGAGACC
CTGTATCTGG CTGTCAACTT CCTGGACAGG TTCCTTTCAT GTATGTCTGT TCTGAGAGGG
AAACTGCAGC TCGTAGGAAC AGCAGCTATG CTTTTGGCTT CGAAATATGA AGAGATATAT
CCTCCTGAAG TAGACGAGTT TGTCTATATC ACCGATGATA CATACACAAA ACGACAACTG
TTAAAAATGG AACACTTGCT TCTGAAAGTT CTAGCTTTTG ATCTGACAGT ACCAACCACC
AACCAGTTTC TCCTTCAGTA CTTGAGGCGA CAAGGAGTGT GCGTCAGGAC TGAGAACCTG
GCTAAGTACG TAGCAGAGCT GAGTCTACTT GAAGCAGATC CATTCTTGAA ATATCTTCCT
TCACTGATAG CTGCAGCAGC TTTTTGCCTG GCAAACTATA CTGTGAACAA GCACTTTTGG
CCAGAAACCC TTGCTGCATT TACAGGGTAT TCATTAAGTG AAATTGTGCC TTGCCTGAGT
GAGCTTCATA AAGCGTACCT TGATATACCC CATCGACCTC AGCAAGCAAT TAGGGAGAAG
TACAAGGCTT CAAAGTACCT GTGTGTGTCC CTCATGGAGC CACCTGCAGT TCTTCTTCTA
CAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25558
Clone ID Related Accession (Same CDS sequence) NM_001111045.1
Accession Version NM_001111045.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1395bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-02
Organism Homo sapiens(Human)
Product cyclin-A1 isoform b
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC396283.1, DC411883.1, BC036346.1 and AA553454.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1, resulting in a protein (isoform b) that is 1 amino acid shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.88683.1, BC035479.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2144333 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGAGACCG GCTTTCCCGC AATCATGTAC CCTGGATCTT TTATTGGGGG CTGGGGAGAA 
GAGTATCTCA GCTGGGAAGG ACCGGGGCTC CCAGATTTCG TCTTCCAGCA GCCCGTGGAG
TCTGAAGCAA TGCACTGCAG CAACCCCAAG AGTGGAGTTG TGCTGGCTAC AGTGGCCCGA
GGTCCCGATG CTTGTCAGAT ACTCACCAGA GCCCCGCTGG GCCAGGATCC CCCGCAGAGG
ACAGTGCTAG GGCTGCTAAC TGCAAATGGG CAGTACAGGA GGACCTGTGG CCAGGGGATC
ACAAGAATCA GGTGTTATTC TGGATCAGAA AATGCCTTCC CTCCAGCTGG AAAGAAAGCA
CTCCCTGACT GTGGGGTCCA AGAGCCCCCC AAGCAAGGGT TTGACATCTA CATGGATGAA
CTAGAGCAGG GGGACAGAGA CAGCTGCTCG GTCAGAGAGG GGATGGCATT TGAGGATGTG
TATGAAGTAG ACACCGGCAC ACTCAAGTCA GACCTGCACT TCCTGCTGGA TTTCAACACA
GTTTCCCCTA TGCTGGTAGA TTCATCTCTC CTCTCCCAGT CTGAAGATAT ATCCAGTCTT
GGCACAGATG TGATAAATGT GACTGAATAT GCTGAAGAAA TTTATCAGTA CCTTAGGGAA
GCTGAAATAA GGCACAGACC CAAAGCACAC TACATGAAGA AGCAGCCAGA CATCACGGAA
GGCATGCGCA CGATTCTGGT GGACTGGCTG GTGGAGGTTG GGGAAGAATA TAAACTTCGA
GCAGAGACCC TGTATCTGGC TGTCAACTTC CTGGACAGGT TCCTTTCATG TATGTCTGTT
CTGAGAGGGA AACTGCAGCT CGTAGGAACA GCAGCTATGC TTTTGGCTTC GAAATATGAA
GAGATATATC CTCCTGAAGT AGACGAGTTT GTCTATATCA CCGATGATAC ATACACAAAA
CGACAACTGT TAAAAATGGA ACACTTGCTT CTGAAAGTTC TAGCTTTTGA TCTGACAGTA
CCAACCACCA ACCAGTTTCT CCTTCAGTAC TTGAGGCGAC AAGGAGTGTG CGTCAGGACT
GAGAACCTGG CTAAGTACGT AGCAGAGCTG AGTCTACTTG AAGCAGATCC ATTCTTGAAA
TATCTTCCTT CACTGATAGC TGCAGCAGCT TTTTGCCTGG CAAACTATAC TGTGAACAAG
CACTTTTGGC CAGAAACCCT TGCTGCATTT ACAGGGTATT CATTAAGTGA AATTGTGCCT
TGCCTGAGTG AGCTTCATAA AGCGTACCTT GATATACCCC ATCGACCTCA GCAAGCAATT
AGGGAGAAGT ACAAGGCTTC AAAGTACCTG TGTGTGTCCC TCATGGAGCC ACCTGCAGTT
CTTCTTCTAC AATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001104515.1
CDS351..1745
Misc Feature(1)240..242
Misc Feature(2)558..>782
Misc Feature(3)987..1367
Misc Feature(4)order(1074..1079,1095..1100,1104..1109,1173..1175,1203..1208)
Misc Feature(5)order(1230..1232,1242..1244,1266..1268,1317..1319,1326..1331)
Misc Feature(6)order(1365..1370,1386..1391,1395..1400,1464..1466,1497..1502)
Misc Feature(7)1371..1724
Misc Feature(8)order(1524..1526,1536..1538,1557..1559,1602..1604,1611..1616)
Exon (1)1..458
Exon (2)459..644
Exon (3)645..891
Exon (4)892..1016
Exon (5)1017..1240
Exon (6)1241..1445
Exon (7)1446..1559
Exon (8)1560..1693
Exon (9)1694..1962
Translation

Target ORF information:

RefSeq Version NM_001111045.1
Organism Homo sapiens(Human)
Definition Homo sapiens cyclin A1 (CCNA1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001111045.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGAGACCG GCTTTCCCGC AATCATGTAC CCTGGATCTT TTATTGGGGG CTGGGGAGAA 
GAGTATCTCA GCTGGGAAGG ACCGGGGCTC CCAGATTTCG TCTTCCAGCA GCCCGTGGAG
TCTGAAGCAA TGCACTGCAG CAACCCCAAG AGTGGAGTTG TGCTGGCTAC AGTGGCCCGA
GGTCCCGATG CTTGTCAGAT ACTCACCAGA GCCCCGCTGG GCCAGGATCC CCCGCAGAGG
ACAGTGCTAG GGCTGCTAAC TGCAAATGGG CAGTACAGGA GGACCTGTGG CCAGGGGATC
ACAAGAATCA GGTGTTATTC TGGATCAGAA AATGCCTTCC CTCCAGCTGG AAAGAAAGCA
CTCCCTGACT GTGGGGTCCA AGAGCCCCCC AAGCAAGGGT TTGACATCTA CATGGATGAA
CTAGAGCAGG GGGACAGAGA CAGCTGCTCG GTCAGAGAGG GGATGGCATT TGAGGATGTG
TATGAAGTAG ACACCGGCAC ACTCAAGTCA GACCTGCACT TCCTGCTGGA TTTCAACACA
GTTTCCCCTA TGCTGGTAGA TTCATCTCTC CTCTCCCAGT CTGAAGATAT ATCCAGTCTT
GGCACAGATG TGATAAATGT GACTGAATAT GCTGAAGAAA TTTATCAGTA CCTTAGGGAA
GCTGAAATAA GGCACAGACC CAAAGCACAC TACATGAAGA AGCAGCCAGA CATCACGGAA
GGCATGCGCA CGATTCTGGT GGACTGGCTG GTGGAGGTTG GGGAAGAATA TAAACTTCGA
GCAGAGACCC TGTATCTGGC TGTCAACTTC CTGGACAGGT TCCTTTCATG TATGTCTGTT
CTGAGAGGGA AACTGCAGCT CGTAGGAACA GCAGCTATGC TTTTGGCTTC GAAATATGAA
GAGATATATC CTCCTGAAGT AGACGAGTTT GTCTATATCA CCGATGATAC ATACACAAAA
CGACAACTGT TAAAAATGGA ACACTTGCTT CTGAAAGTTC TAGCTTTTGA TCTGACAGTA
CCAACCACCA ACCAGTTTCT CCTTCAGTAC TTGAGGCGAC AAGGAGTGTG CGTCAGGACT
GAGAACCTGG CTAAGTACGT AGCAGAGCTG AGTCTACTTG AAGCAGATCC ATTCTTGAAA
TATCTTCCTT CACTGATAGC TGCAGCAGCT TTTTGCCTGG CAAACTATAC TGTGAACAAG
CACTTTTGGC CAGAAACCCT TGCTGCATTT ACAGGGTATT CATTAAGTGA AATTGTGCCT
TGCCTGAGTG AGCTTCATAA AGCGTACCTT GATATACCCC ATCGACCTCA GCAAGCAATT
AGGGAGAAGT ACAAGGCTTC AAAGTACCTG TGTGTGTCCC TCATGGAGCC ACCTGCAGTT
CTTCTTCTAC AATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25352
Clone ID Related Accession (Same CDS sequence) XM_011535296.2 , XM_011535294.1 , XM_011535297.1 , XM_011535296.1 , NM_001111046.1 , NM_001111047.1 , XM_011535294.2 , XM_011535295.2 , XM_011535295.1
Accession Version XM_011535296.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1266bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product cyclin-A1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011535296.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGCACTGCA GCAACCCCAA GAGTGGAGTT GTGCTGGCTA CAGTGGCCCG AGGTCCCGAT 
GCTTGTCAGA TACTCACCAG AGCCCCGCTG GGCCAGGATC CCCCGCAGAG GACAGTGCTA
GGGCTGCTAA CTGCAAATGG GCAGTACAGG AGGACCTGTG GCCAGGGGAT CACAAGAATC
AGGTGTTATT CTGGATCAGA AAATGCCTTC CCTCCAGCTG GAAAGAAAGC ACTCCCTGAC
TGTGGGGTCC AAGAGCCCCC CAAGCAAGGG TTTGACATCT ACATGGATGA ACTAGAGCAG
GGGGACAGAG ACAGCTGCTC GGTCAGAGAG GGGATGGCAT TTGAGGATGT GTATGAAGTA
GACACCGGCA CACTCAAGTC AGACCTGCAC TTCCTGCTGG ATTTCAACAC AGTTTCCCCT
ATGCTGGTAG ATTCATCTCT CCTCTCCCAG TCTGAAGATA TATCCAGTCT TGGCACAGAT
GTGATAAATG TGACTGAATA TGCTGAAGAA ATTTATCAGT ACCTTAGGGA AGCTGAAATA
AGGCACAGAC CCAAAGCACA CTACATGAAG AAGCAGCCAG ACATCACGGA AGGCATGCGC
ACGATTCTGG TGGACTGGCT GGTGGAGGTT GGGGAAGAAT ATAAACTTCG AGCAGAGACC
CTGTATCTGG CTGTCAACTT CCTGGACAGG TTCCTTTCAT GTATGTCTGT TCTGAGAGGG
AAACTGCAGC TCGTAGGAAC AGCAGCTATG CTTTTGGCTT CGAAATATGA AGAGATATAT
CCTCCTGAAG TAGACGAGTT TGTCTATATC ACCGATGATA CATACACAAA ACGACAACTG
TTAAAAATGG AACACTTGCT TCTGAAAGTT CTAGCTTTTG ATCTGACAGT ACCAACCACC
AACCAGTTTC TCCTTCAGTA CTTGAGGCGA CAAGGAGTGT GCGTCAGGAC TGAGAACCTG
GCTAAGTACG TAGCAGAGCT GAGTCTACTT GAAGCAGATC CATTCTTGAA ATATCTTCCT
TCACTGATAG CTGCAGCAGC TTTTTGCCTG GCAAACTATA CTGTGAACAA GCACTTTTGG
CCAGAAACCC TTGCTGCATT TACAGGGTAT TCATTAAGTG AAATTGTGCC TTGCCTGAGT
GAGCTTCATA AAGCGTACCT TGATATACCC CATCGACCTC AGCAAGCAAT TAGGGAGAAG
TACAAGGCTT CAAAGTACCT GTGTGTGTCC CTCATGGAGC CACCTGCAGT TCTTCTTCTA
CAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011533598.1
CDS139..1404
Misc Feature(1)217..>441
Misc Feature(2)646..1026
Misc Feature(3)order(733..738,754..759,763..768,832..834,862..867)
Misc Feature(4)order(889..891,901..903,925..927,976..978,985..990)
Misc Feature(5)order(1024..1029,1045..1050,1054..1059,1123..1125,1156..1161)
Misc Feature(6)1030..1383
Misc Feature(7)order(1183..1185,1195..1197,1216..1218,1261..1263,1270..1275)
Translation

Target ORF information:

RefSeq Version XM_011535296.2
Organism Homo sapiens(Human)
Definition Homo sapiens cyclin A1 (CCNA1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011535296.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGCACTGCA GCAACCCCAA GAGTGGAGTT GTGCTGGCTA CAGTGGCCCG AGGTCCCGAT 
GCTTGTCAGA TACTCACCAG AGCCCCGCTG GGCCAGGATC CCCCGCAGAG GACAGTGCTA
GGGCTGCTAA CTGCAAATGG GCAGTACAGG AGGACCTGTG GCCAGGGGAT CACAAGAATC
AGGTGTTATT CTGGATCAGA AAATGCCTTC CCTCCAGCTG GAAAGAAAGC ACTCCCTGAC
TGTGGGGTCC AAGAGCCCCC CAAGCAAGGG TTTGACATCT ACATGGATGA ACTAGAGCAG
GGGGACAGAG ACAGCTGCTC GGTCAGAGAG GGGATGGCAT TTGAGGATGT GTATGAAGTA
GACACCGGCA CACTCAAGTC AGACCTGCAC TTCCTGCTGG ATTTCAACAC AGTTTCCCCT
ATGCTGGTAG ATTCATCTCT CCTCTCCCAG TCTGAAGATA TATCCAGTCT TGGCACAGAT
GTGATAAATG TGACTGAATA TGCTGAAGAA ATTTATCAGT ACCTTAGGGA AGCTGAAATA
AGGCACAGAC CCAAAGCACA CTACATGAAG AAGCAGCCAG ACATCACGGA AGGCATGCGC
ACGATTCTGG TGGACTGGCT GGTGGAGGTT GGGGAAGAAT ATAAACTTCG AGCAGAGACC
CTGTATCTGG CTGTCAACTT CCTGGACAGG TTCCTTTCAT GTATGTCTGT TCTGAGAGGG
AAACTGCAGC TCGTAGGAAC AGCAGCTATG CTTTTGGCTT CGAAATATGA AGAGATATAT
CCTCCTGAAG TAGACGAGTT TGTCTATATC ACCGATGATA CATACACAAA ACGACAACTG
TTAAAAATGG AACACTTGCT TCTGAAAGTT CTAGCTTTTG ATCTGACAGT ACCAACCACC
AACCAGTTTC TCCTTCAGTA CTTGAGGCGA CAAGGAGTGT GCGTCAGGAC TGAGAACCTG
GCTAAGTACG TAGCAGAGCT GAGTCTACTT GAAGCAGATC CATTCTTGAA ATATCTTCCT
TCACTGATAG CTGCAGCAGC TTTTTGCCTG GCAAACTATA CTGTGAACAA GCACTTTTGG
CCAGAAACCC TTGCTGCATT TACAGGGTAT TCATTAAGTG AAATTGTGCC TTGCCTGAGT
GAGCTTCATA AAGCGTACCT TGATATACCC CATCGACCTC AGCAAGCAAT TAGGGAGAAG
TACAAGGCTT CAAAGTACCT GTGTGTGTCC CTCATGGAGC CACCTGCAGT TCTTCTTCTA
CAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25352
Clone ID Related Accession (Same CDS sequence) XM_011535296.2 , XM_011535294.1 , XM_011535297.1 , XM_011535296.1 , NM_001111046.1 , NM_001111047.1 , XM_011535294.2 , XM_011535295.2 , XM_011535295.1
Accession Version XM_011535294.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1266bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-03-12
Organism Homo sapiens(Human)
Product cyclin-A1 isoform X1
Comment

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGCACTGCA GCAACCCCAA GAGTGGAGTT GTGCTGGCTA CAGTGGCCCG AGGTCCCGAT 
GCTTGTCAGA TACTCACCAG AGCCCCGCTG GGCCAGGATC CCCCGCAGAG GACAGTGCTA
GGGCTGCTAA CTGCAAATGG GCAGTACAGG AGGACCTGTG GCCAGGGGAT CACAAGAATC
AGGTGTTATT CTGGATCAGA AAATGCCTTC CCTCCAGCTG GAAAGAAAGC ACTCCCTGAC
TGTGGGGTCC AAGAGCCCCC CAAGCAAGGG TTTGACATCT ACATGGATGA ACTAGAGCAG
GGGGACAGAG ACAGCTGCTC GGTCAGAGAG GGGATGGCAT TTGAGGATGT GTATGAAGTA
GACACCGGCA CACTCAAGTC AGACCTGCAC TTCCTGCTGG ATTTCAACAC AGTTTCCCCT
ATGCTGGTAG ATTCATCTCT CCTCTCCCAG TCTGAAGATA TATCCAGTCT TGGCACAGAT
GTGATAAATG TGACTGAATA TGCTGAAGAA ATTTATCAGT ACCTTAGGGA AGCTGAAATA
AGGCACAGAC CCAAAGCACA CTACATGAAG AAGCAGCCAG ACATCACGGA AGGCATGCGC
ACGATTCTGG TGGACTGGCT GGTGGAGGTT GGGGAAGAAT ATAAACTTCG AGCAGAGACC
CTGTATCTGG CTGTCAACTT CCTGGACAGG TTCCTTTCAT GTATGTCTGT TCTGAGAGGG
AAACTGCAGC TCGTAGGAAC AGCAGCTATG CTTTTGGCTT CGAAATATGA AGAGATATAT
CCTCCTGAAG TAGACGAGTT TGTCTATATC ACCGATGATA CATACACAAA ACGACAACTG
TTAAAAATGG AACACTTGCT TCTGAAAGTT CTAGCTTTTG ATCTGACAGT ACCAACCACC
AACCAGTTTC TCCTTCAGTA CTTGAGGCGA CAAGGAGTGT GCGTCAGGAC TGAGAACCTG
GCTAAGTACG TAGCAGAGCT GAGTCTACTT GAAGCAGATC CATTCTTGAA ATATCTTCCT
TCACTGATAG CTGCAGCAGC TTTTTGCCTG GCAAACTATA CTGTGAACAA GCACTTTTGG
CCAGAAACCC TTGCTGCATT TACAGGGTAT TCATTAAGTG AAATTGTGCC TTGCCTGAGT
GAGCTTCATA AAGCGTACCT TGATATACCC CATCGACCTC AGCAAGCAAT TAGGGAGAAG
TACAAGGCTT CAAAGTACCT GTGTGTGTCC CTCATGGAGC CACCTGCAGT TCTTCTTCTA
CAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011533596.1
Translation

Target ORF information:

RefSeq Version XM_011535294.1
Organism Homo sapiens(Human)
Definition Homo sapiens cyclin A1 (CCNA1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011535294.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGCACTGCA GCAACCCCAA GAGTGGAGTT GTGCTGGCTA CAGTGGCCCG AGGTCCCGAT 
GCTTGTCAGA TACTCACCAG AGCCCCGCTG GGCCAGGATC CCCCGCAGAG GACAGTGCTA
GGGCTGCTAA CTGCAAATGG GCAGTACAGG AGGACCTGTG GCCAGGGGAT CACAAGAATC
AGGTGTTATT CTGGATCAGA AAATGCCTTC CCTCCAGCTG GAAAGAAAGC ACTCCCTGAC
TGTGGGGTCC AAGAGCCCCC CAAGCAAGGG TTTGACATCT ACATGGATGA ACTAGAGCAG
GGGGACAGAG ACAGCTGCTC GGTCAGAGAG GGGATGGCAT TTGAGGATGT GTATGAAGTA
GACACCGGCA CACTCAAGTC AGACCTGCAC TTCCTGCTGG ATTTCAACAC AGTTTCCCCT
ATGCTGGTAG ATTCATCTCT CCTCTCCCAG TCTGAAGATA TATCCAGTCT TGGCACAGAT
GTGATAAATG TGACTGAATA TGCTGAAGAA ATTTATCAGT ACCTTAGGGA AGCTGAAATA
AGGCACAGAC CCAAAGCACA CTACATGAAG AAGCAGCCAG ACATCACGGA AGGCATGCGC
ACGATTCTGG TGGACTGGCT GGTGGAGGTT GGGGAAGAAT ATAAACTTCG AGCAGAGACC
CTGTATCTGG CTGTCAACTT CCTGGACAGG TTCCTTTCAT GTATGTCTGT TCTGAGAGGG
AAACTGCAGC TCGTAGGAAC AGCAGCTATG CTTTTGGCTT CGAAATATGA AGAGATATAT
CCTCCTGAAG TAGACGAGTT TGTCTATATC ACCGATGATA CATACACAAA ACGACAACTG
TTAAAAATGG AACACTTGCT TCTGAAAGTT CTAGCTTTTG ATCTGACAGT ACCAACCACC
AACCAGTTTC TCCTTCAGTA CTTGAGGCGA CAAGGAGTGT GCGTCAGGAC TGAGAACCTG
GCTAAGTACG TAGCAGAGCT GAGTCTACTT GAAGCAGATC CATTCTTGAA ATATCTTCCT
TCACTGATAG CTGCAGCAGC TTTTTGCCTG GCAAACTATA CTGTGAACAA GCACTTTTGG
CCAGAAACCC TTGCTGCATT TACAGGGTAT TCATTAAGTG AAATTGTGCC TTGCCTGAGT
GAGCTTCATA AAGCGTACCT TGATATACCC CATCGACCTC AGCAAGCAAT TAGGGAGAAG
TACAAGGCTT CAAAGTACCT GTGTGTGTCC CTCATGGAGC CACCTGCAGT TCTTCTTCTA
CAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25352
Clone ID Related Accession (Same CDS sequence) XM_011535296.2 , XM_011535294.1 , XM_011535297.1 , XM_011535296.1 , NM_001111046.1 , NM_001111047.1 , XM_011535294.2 , XM_011535295.2 , XM_011535295.1
Accession Version XM_011535297.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1266bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-03-12
Organism Homo sapiens(Human)
Product cyclin-A1 isoform X1
Comment

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGCACTGCA GCAACCCCAA GAGTGGAGTT GTGCTGGCTA CAGTGGCCCG AGGTCCCGAT 
GCTTGTCAGA TACTCACCAG AGCCCCGCTG GGCCAGGATC CCCCGCAGAG GACAGTGCTA
GGGCTGCTAA CTGCAAATGG GCAGTACAGG AGGACCTGTG GCCAGGGGAT CACAAGAATC
AGGTGTTATT CTGGATCAGA AAATGCCTTC CCTCCAGCTG GAAAGAAAGC ACTCCCTGAC
TGTGGGGTCC AAGAGCCCCC CAAGCAAGGG TTTGACATCT ACATGGATGA ACTAGAGCAG
GGGGACAGAG ACAGCTGCTC GGTCAGAGAG GGGATGGCAT TTGAGGATGT GTATGAAGTA
GACACCGGCA CACTCAAGTC AGACCTGCAC TTCCTGCTGG ATTTCAACAC AGTTTCCCCT
ATGCTGGTAG ATTCATCTCT CCTCTCCCAG TCTGAAGATA TATCCAGTCT TGGCACAGAT
GTGATAAATG TGACTGAATA TGCTGAAGAA ATTTATCAGT ACCTTAGGGA AGCTGAAATA
AGGCACAGAC CCAAAGCACA CTACATGAAG AAGCAGCCAG ACATCACGGA AGGCATGCGC
ACGATTCTGG TGGACTGGCT GGTGGAGGTT GGGGAAGAAT ATAAACTTCG AGCAGAGACC
CTGTATCTGG CTGTCAACTT CCTGGACAGG TTCCTTTCAT GTATGTCTGT TCTGAGAGGG
AAACTGCAGC TCGTAGGAAC AGCAGCTATG CTTTTGGCTT CGAAATATGA AGAGATATAT
CCTCCTGAAG TAGACGAGTT TGTCTATATC ACCGATGATA CATACACAAA ACGACAACTG
TTAAAAATGG AACACTTGCT TCTGAAAGTT CTAGCTTTTG ATCTGACAGT ACCAACCACC
AACCAGTTTC TCCTTCAGTA CTTGAGGCGA CAAGGAGTGT GCGTCAGGAC TGAGAACCTG
GCTAAGTACG TAGCAGAGCT GAGTCTACTT GAAGCAGATC CATTCTTGAA ATATCTTCCT
TCACTGATAG CTGCAGCAGC TTTTTGCCTG GCAAACTATA CTGTGAACAA GCACTTTTGG
CCAGAAACCC TTGCTGCATT TACAGGGTAT TCATTAAGTG AAATTGTGCC TTGCCTGAGT
GAGCTTCATA AAGCGTACCT TGATATACCC CATCGACCTC AGCAAGCAAT TAGGGAGAAG
TACAAGGCTT CAAAGTACCT GTGTGTGTCC CTCATGGAGC CACCTGCAGT TCTTCTTCTA
CAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011533599.1
Translation

Target ORF information:

RefSeq Version XM_011535297.1
Organism Homo sapiens(Human)
Definition Homo sapiens cyclin A1 (CCNA1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011535297.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGCACTGCA GCAACCCCAA GAGTGGAGTT GTGCTGGCTA CAGTGGCCCG AGGTCCCGAT 
GCTTGTCAGA TACTCACCAG AGCCCCGCTG GGCCAGGATC CCCCGCAGAG GACAGTGCTA
GGGCTGCTAA CTGCAAATGG GCAGTACAGG AGGACCTGTG GCCAGGGGAT CACAAGAATC
AGGTGTTATT CTGGATCAGA AAATGCCTTC CCTCCAGCTG GAAAGAAAGC ACTCCCTGAC
TGTGGGGTCC AAGAGCCCCC CAAGCAAGGG TTTGACATCT ACATGGATGA ACTAGAGCAG
GGGGACAGAG ACAGCTGCTC GGTCAGAGAG GGGATGGCAT TTGAGGATGT GTATGAAGTA
GACACCGGCA CACTCAAGTC AGACCTGCAC TTCCTGCTGG ATTTCAACAC AGTTTCCCCT
ATGCTGGTAG ATTCATCTCT CCTCTCCCAG TCTGAAGATA TATCCAGTCT TGGCACAGAT
GTGATAAATG TGACTGAATA TGCTGAAGAA ATTTATCAGT ACCTTAGGGA AGCTGAAATA
AGGCACAGAC CCAAAGCACA CTACATGAAG AAGCAGCCAG ACATCACGGA AGGCATGCGC
ACGATTCTGG TGGACTGGCT GGTGGAGGTT GGGGAAGAAT ATAAACTTCG AGCAGAGACC
CTGTATCTGG CTGTCAACTT CCTGGACAGG TTCCTTTCAT GTATGTCTGT TCTGAGAGGG
AAACTGCAGC TCGTAGGAAC AGCAGCTATG CTTTTGGCTT CGAAATATGA AGAGATATAT
CCTCCTGAAG TAGACGAGTT TGTCTATATC ACCGATGATA CATACACAAA ACGACAACTG
TTAAAAATGG AACACTTGCT TCTGAAAGTT CTAGCTTTTG ATCTGACAGT ACCAACCACC
AACCAGTTTC TCCTTCAGTA CTTGAGGCGA CAAGGAGTGT GCGTCAGGAC TGAGAACCTG
GCTAAGTACG TAGCAGAGCT GAGTCTACTT GAAGCAGATC CATTCTTGAA ATATCTTCCT
TCACTGATAG CTGCAGCAGC TTTTTGCCTG GCAAACTATA CTGTGAACAA GCACTTTTGG
CCAGAAACCC TTGCTGCATT TACAGGGTAT TCATTAAGTG AAATTGTGCC TTGCCTGAGT
GAGCTTCATA AAGCGTACCT TGATATACCC CATCGACCTC AGCAAGCAAT TAGGGAGAAG
TACAAGGCTT CAAAGTACCT GTGTGTGTCC CTCATGGAGC CACCTGCAGT TCTTCTTCTA
CAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25352
Clone ID Related Accession (Same CDS sequence) XM_011535296.2 , XM_011535294.1 , XM_011535297.1 , XM_011535296.1 , NM_001111046.1 , NM_001111047.1 , XM_011535294.2 , XM_011535295.2 , XM_011535295.1
Accession Version XM_011535296.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1266bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-03-12
Organism Homo sapiens(Human)
Product cyclin-A1 isoform X1
Comment

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGCACTGCA GCAACCCCAA GAGTGGAGTT GTGCTGGCTA CAGTGGCCCG AGGTCCCGAT 
GCTTGTCAGA TACTCACCAG AGCCCCGCTG GGCCAGGATC CCCCGCAGAG GACAGTGCTA
GGGCTGCTAA CTGCAAATGG GCAGTACAGG AGGACCTGTG GCCAGGGGAT CACAAGAATC
AGGTGTTATT CTGGATCAGA AAATGCCTTC CCTCCAGCTG GAAAGAAAGC ACTCCCTGAC
TGTGGGGTCC AAGAGCCCCC CAAGCAAGGG TTTGACATCT ACATGGATGA ACTAGAGCAG
GGGGACAGAG ACAGCTGCTC GGTCAGAGAG GGGATGGCAT TTGAGGATGT GTATGAAGTA
GACACCGGCA CACTCAAGTC AGACCTGCAC TTCCTGCTGG ATTTCAACAC AGTTTCCCCT
ATGCTGGTAG ATTCATCTCT CCTCTCCCAG TCTGAAGATA TATCCAGTCT TGGCACAGAT
GTGATAAATG TGACTGAATA TGCTGAAGAA ATTTATCAGT ACCTTAGGGA AGCTGAAATA
AGGCACAGAC CCAAAGCACA CTACATGAAG AAGCAGCCAG ACATCACGGA AGGCATGCGC
ACGATTCTGG TGGACTGGCT GGTGGAGGTT GGGGAAGAAT ATAAACTTCG AGCAGAGACC
CTGTATCTGG CTGTCAACTT CCTGGACAGG TTCCTTTCAT GTATGTCTGT TCTGAGAGGG
AAACTGCAGC TCGTAGGAAC AGCAGCTATG CTTTTGGCTT CGAAATATGA AGAGATATAT
CCTCCTGAAG TAGACGAGTT TGTCTATATC ACCGATGATA CATACACAAA ACGACAACTG
TTAAAAATGG AACACTTGCT TCTGAAAGTT CTAGCTTTTG ATCTGACAGT ACCAACCACC
AACCAGTTTC TCCTTCAGTA CTTGAGGCGA CAAGGAGTGT GCGTCAGGAC TGAGAACCTG
GCTAAGTACG TAGCAGAGCT GAGTCTACTT GAAGCAGATC CATTCTTGAA ATATCTTCCT
TCACTGATAG CTGCAGCAGC TTTTTGCCTG GCAAACTATA CTGTGAACAA GCACTTTTGG
CCAGAAACCC TTGCTGCATT TACAGGGTAT TCATTAAGTG AAATTGTGCC TTGCCTGAGT
GAGCTTCATA AAGCGTACCT TGATATACCC CATCGACCTC AGCAAGCAAT TAGGGAGAAG
TACAAGGCTT CAAAGTACCT GTGTGTGTCC CTCATGGAGC CACCTGCAGT TCTTCTTCTA
CAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011533598.1
Translation

Target ORF information:

RefSeq Version XM_011535296.1
Organism Homo sapiens(Human)
Definition Homo sapiens cyclin A1 (CCNA1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011535296.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGCACTGCA GCAACCCCAA GAGTGGAGTT GTGCTGGCTA CAGTGGCCCG AGGTCCCGAT 
GCTTGTCAGA TACTCACCAG AGCCCCGCTG GGCCAGGATC CCCCGCAGAG GACAGTGCTA
GGGCTGCTAA CTGCAAATGG GCAGTACAGG AGGACCTGTG GCCAGGGGAT CACAAGAATC
AGGTGTTATT CTGGATCAGA AAATGCCTTC CCTCCAGCTG GAAAGAAAGC ACTCCCTGAC
TGTGGGGTCC AAGAGCCCCC CAAGCAAGGG TTTGACATCT ACATGGATGA ACTAGAGCAG
GGGGACAGAG ACAGCTGCTC GGTCAGAGAG GGGATGGCAT TTGAGGATGT GTATGAAGTA
GACACCGGCA CACTCAAGTC AGACCTGCAC TTCCTGCTGG ATTTCAACAC AGTTTCCCCT
ATGCTGGTAG ATTCATCTCT CCTCTCCCAG TCTGAAGATA TATCCAGTCT TGGCACAGAT
GTGATAAATG TGACTGAATA TGCTGAAGAA ATTTATCAGT ACCTTAGGGA AGCTGAAATA
AGGCACAGAC CCAAAGCACA CTACATGAAG AAGCAGCCAG ACATCACGGA AGGCATGCGC
ACGATTCTGG TGGACTGGCT GGTGGAGGTT GGGGAAGAAT ATAAACTTCG AGCAGAGACC
CTGTATCTGG CTGTCAACTT CCTGGACAGG TTCCTTTCAT GTATGTCTGT TCTGAGAGGG
AAACTGCAGC TCGTAGGAAC AGCAGCTATG CTTTTGGCTT CGAAATATGA AGAGATATAT
CCTCCTGAAG TAGACGAGTT TGTCTATATC ACCGATGATA CATACACAAA ACGACAACTG
TTAAAAATGG AACACTTGCT TCTGAAAGTT CTAGCTTTTG ATCTGACAGT ACCAACCACC
AACCAGTTTC TCCTTCAGTA CTTGAGGCGA CAAGGAGTGT GCGTCAGGAC TGAGAACCTG
GCTAAGTACG TAGCAGAGCT GAGTCTACTT GAAGCAGATC CATTCTTGAA ATATCTTCCT
TCACTGATAG CTGCAGCAGC TTTTTGCCTG GCAAACTATA CTGTGAACAA GCACTTTTGG
CCAGAAACCC TTGCTGCATT TACAGGGTAT TCATTAAGTG AAATTGTGCC TTGCCTGAGT
GAGCTTCATA AAGCGTACCT TGATATACCC CATCGACCTC AGCAAGCAAT TAGGGAGAAG
TACAAGGCTT CAAAGTACCT GTGTGTGTCC CTCATGGAGC CACCTGCAGT TCTTCTTCTA
CAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25352
Clone ID Related Accession (Same CDS sequence) XM_011535296.2 , XM_011535294.1 , XM_011535297.1 , XM_011535296.1 , NM_001111046.1 , NM_001111047.1 , XM_011535294.2 , XM_011535295.2 , XM_011535295.1
Accession Version XM_011535294.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1266bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product cyclin-A1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011535294.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGCACTGCA GCAACCCCAA GAGTGGAGTT GTGCTGGCTA CAGTGGCCCG AGGTCCCGAT 
GCTTGTCAGA TACTCACCAG AGCCCCGCTG GGCCAGGATC CCCCGCAGAG GACAGTGCTA
GGGCTGCTAA CTGCAAATGG GCAGTACAGG AGGACCTGTG GCCAGGGGAT CACAAGAATC
AGGTGTTATT CTGGATCAGA AAATGCCTTC CCTCCAGCTG GAAAGAAAGC ACTCCCTGAC
TGTGGGGTCC AAGAGCCCCC CAAGCAAGGG TTTGACATCT ACATGGATGA ACTAGAGCAG
GGGGACAGAG ACAGCTGCTC GGTCAGAGAG GGGATGGCAT TTGAGGATGT GTATGAAGTA
GACACCGGCA CACTCAAGTC AGACCTGCAC TTCCTGCTGG ATTTCAACAC AGTTTCCCCT
ATGCTGGTAG ATTCATCTCT CCTCTCCCAG TCTGAAGATA TATCCAGTCT TGGCACAGAT
GTGATAAATG TGACTGAATA TGCTGAAGAA ATTTATCAGT ACCTTAGGGA AGCTGAAATA
AGGCACAGAC CCAAAGCACA CTACATGAAG AAGCAGCCAG ACATCACGGA AGGCATGCGC
ACGATTCTGG TGGACTGGCT GGTGGAGGTT GGGGAAGAAT ATAAACTTCG AGCAGAGACC
CTGTATCTGG CTGTCAACTT CCTGGACAGG TTCCTTTCAT GTATGTCTGT TCTGAGAGGG
AAACTGCAGC TCGTAGGAAC AGCAGCTATG CTTTTGGCTT CGAAATATGA AGAGATATAT
CCTCCTGAAG TAGACGAGTT TGTCTATATC ACCGATGATA CATACACAAA ACGACAACTG
TTAAAAATGG AACACTTGCT TCTGAAAGTT CTAGCTTTTG ATCTGACAGT ACCAACCACC
AACCAGTTTC TCCTTCAGTA CTTGAGGCGA CAAGGAGTGT GCGTCAGGAC TGAGAACCTG
GCTAAGTACG TAGCAGAGCT GAGTCTACTT GAAGCAGATC CATTCTTGAA ATATCTTCCT
TCACTGATAG CTGCAGCAGC TTTTTGCCTG GCAAACTATA CTGTGAACAA GCACTTTTGG
CCAGAAACCC TTGCTGCATT TACAGGGTAT TCATTAAGTG AAATTGTGCC TTGCCTGAGT
GAGCTTCATA AAGCGTACCT TGATATACCC CATCGACCTC AGCAAGCAAT TAGGGAGAAG
TACAAGGCTT CAAAGTACCT GTGTGTGTCC CTCATGGAGC CACCTGCAGT TCTTCTTCTA
CAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011533596.1
CDS1314..2579
Misc Feature(1)1392..>1616
Misc Feature(2)1821..2201
Misc Feature(3)order(1908..1913,1929..1934,1938..1943,2007..2009,2037..2042)
Misc Feature(4)order(2064..2066,2076..2078,2100..2102,2151..2153,2160..2165)
Misc Feature(5)order(2199..2204,2220..2225,2229..2234,2298..2300,2331..2336)
Misc Feature(6)2205..2558
Misc Feature(7)order(2358..2360,2370..2372,2391..2393,2436..2438,2445..2450)
Translation

Target ORF information:

RefSeq Version XM_011535294.2
Organism Homo sapiens(Human)
Definition Homo sapiens cyclin A1 (CCNA1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011535294.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGCACTGCA GCAACCCCAA GAGTGGAGTT GTGCTGGCTA CAGTGGCCCG AGGTCCCGAT 
GCTTGTCAGA TACTCACCAG AGCCCCGCTG GGCCAGGATC CCCCGCAGAG GACAGTGCTA
GGGCTGCTAA CTGCAAATGG GCAGTACAGG AGGACCTGTG GCCAGGGGAT CACAAGAATC
AGGTGTTATT CTGGATCAGA AAATGCCTTC CCTCCAGCTG GAAAGAAAGC ACTCCCTGAC
TGTGGGGTCC AAGAGCCCCC CAAGCAAGGG TTTGACATCT ACATGGATGA ACTAGAGCAG
GGGGACAGAG ACAGCTGCTC GGTCAGAGAG GGGATGGCAT TTGAGGATGT GTATGAAGTA
GACACCGGCA CACTCAAGTC AGACCTGCAC TTCCTGCTGG ATTTCAACAC AGTTTCCCCT
ATGCTGGTAG ATTCATCTCT CCTCTCCCAG TCTGAAGATA TATCCAGTCT TGGCACAGAT
GTGATAAATG TGACTGAATA TGCTGAAGAA ATTTATCAGT ACCTTAGGGA AGCTGAAATA
AGGCACAGAC CCAAAGCACA CTACATGAAG AAGCAGCCAG ACATCACGGA AGGCATGCGC
ACGATTCTGG TGGACTGGCT GGTGGAGGTT GGGGAAGAAT ATAAACTTCG AGCAGAGACC
CTGTATCTGG CTGTCAACTT CCTGGACAGG TTCCTTTCAT GTATGTCTGT TCTGAGAGGG
AAACTGCAGC TCGTAGGAAC AGCAGCTATG CTTTTGGCTT CGAAATATGA AGAGATATAT
CCTCCTGAAG TAGACGAGTT TGTCTATATC ACCGATGATA CATACACAAA ACGACAACTG
TTAAAAATGG AACACTTGCT TCTGAAAGTT CTAGCTTTTG ATCTGACAGT ACCAACCACC
AACCAGTTTC TCCTTCAGTA CTTGAGGCGA CAAGGAGTGT GCGTCAGGAC TGAGAACCTG
GCTAAGTACG TAGCAGAGCT GAGTCTACTT GAAGCAGATC CATTCTTGAA ATATCTTCCT
TCACTGATAG CTGCAGCAGC TTTTTGCCTG GCAAACTATA CTGTGAACAA GCACTTTTGG
CCAGAAACCC TTGCTGCATT TACAGGGTAT TCATTAAGTG AAATTGTGCC TTGCCTGAGT
GAGCTTCATA AAGCGTACCT TGATATACCC CATCGACCTC AGCAAGCAAT TAGGGAGAAG
TACAAGGCTT CAAAGTACCT GTGTGTGTCC CTCATGGAGC CACCTGCAGT TCTTCTTCTA
CAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25352
Clone ID Related Accession (Same CDS sequence) XM_011535296.2 , XM_011535294.1 , XM_011535297.1 , XM_011535296.1 , NM_001111046.1 , NM_001111047.1 , XM_011535294.2 , XM_011535295.2 , XM_011535295.1
Accession Version XM_011535295.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1266bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product cyclin-A1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011535295.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGCACTGCA GCAACCCCAA GAGTGGAGTT GTGCTGGCTA CAGTGGCCCG AGGTCCCGAT 
GCTTGTCAGA TACTCACCAG AGCCCCGCTG GGCCAGGATC CCCCGCAGAG GACAGTGCTA
GGGCTGCTAA CTGCAAATGG GCAGTACAGG AGGACCTGTG GCCAGGGGAT CACAAGAATC
AGGTGTTATT CTGGATCAGA AAATGCCTTC CCTCCAGCTG GAAAGAAAGC ACTCCCTGAC
TGTGGGGTCC AAGAGCCCCC CAAGCAAGGG TTTGACATCT ACATGGATGA ACTAGAGCAG
GGGGACAGAG ACAGCTGCTC GGTCAGAGAG GGGATGGCAT TTGAGGATGT GTATGAAGTA
GACACCGGCA CACTCAAGTC AGACCTGCAC TTCCTGCTGG ATTTCAACAC AGTTTCCCCT
ATGCTGGTAG ATTCATCTCT CCTCTCCCAG TCTGAAGATA TATCCAGTCT TGGCACAGAT
GTGATAAATG TGACTGAATA TGCTGAAGAA ATTTATCAGT ACCTTAGGGA AGCTGAAATA
AGGCACAGAC CCAAAGCACA CTACATGAAG AAGCAGCCAG ACATCACGGA AGGCATGCGC
ACGATTCTGG TGGACTGGCT GGTGGAGGTT GGGGAAGAAT ATAAACTTCG AGCAGAGACC
CTGTATCTGG CTGTCAACTT CCTGGACAGG TTCCTTTCAT GTATGTCTGT TCTGAGAGGG
AAACTGCAGC TCGTAGGAAC AGCAGCTATG CTTTTGGCTT CGAAATATGA AGAGATATAT
CCTCCTGAAG TAGACGAGTT TGTCTATATC ACCGATGATA CATACACAAA ACGACAACTG
TTAAAAATGG AACACTTGCT TCTGAAAGTT CTAGCTTTTG ATCTGACAGT ACCAACCACC
AACCAGTTTC TCCTTCAGTA CTTGAGGCGA CAAGGAGTGT GCGTCAGGAC TGAGAACCTG
GCTAAGTACG TAGCAGAGCT GAGTCTACTT GAAGCAGATC CATTCTTGAA ATATCTTCCT
TCACTGATAG CTGCAGCAGC TTTTTGCCTG GCAAACTATA CTGTGAACAA GCACTTTTGG
CCAGAAACCC TTGCTGCATT TACAGGGTAT TCATTAAGTG AAATTGTGCC TTGCCTGAGT
GAGCTTCATA AAGCGTACCT TGATATACCC CATCGACCTC AGCAAGCAAT TAGGGAGAAG
TACAAGGCTT CAAAGTACCT GTGTGTGTCC CTCATGGAGC CACCTGCAGT TCTTCTTCTA
CAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011533597.1
CDS1311..2576
Misc Feature(1)1389..>1613
Misc Feature(2)1818..2198
Misc Feature(3)order(1905..1910,1926..1931,1935..1940,2004..2006,2034..2039)
Misc Feature(4)order(2061..2063,2073..2075,2097..2099,2148..2150,2157..2162)
Misc Feature(5)order(2196..2201,2217..2222,2226..2231,2295..2297,2328..2333)
Misc Feature(6)2202..2555
Misc Feature(7)order(2355..2357,2367..2369,2388..2390,2433..2435,2442..2447)
Translation

Target ORF information:

RefSeq Version XM_011535295.2
Organism Homo sapiens(Human)
Definition Homo sapiens cyclin A1 (CCNA1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011535295.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGCACTGCA GCAACCCCAA GAGTGGAGTT GTGCTGGCTA CAGTGGCCCG AGGTCCCGAT 
GCTTGTCAGA TACTCACCAG AGCCCCGCTG GGCCAGGATC CCCCGCAGAG GACAGTGCTA
GGGCTGCTAA CTGCAAATGG GCAGTACAGG AGGACCTGTG GCCAGGGGAT CACAAGAATC
AGGTGTTATT CTGGATCAGA AAATGCCTTC CCTCCAGCTG GAAAGAAAGC ACTCCCTGAC
TGTGGGGTCC AAGAGCCCCC CAAGCAAGGG TTTGACATCT ACATGGATGA ACTAGAGCAG
GGGGACAGAG ACAGCTGCTC GGTCAGAGAG GGGATGGCAT TTGAGGATGT GTATGAAGTA
GACACCGGCA CACTCAAGTC AGACCTGCAC TTCCTGCTGG ATTTCAACAC AGTTTCCCCT
ATGCTGGTAG ATTCATCTCT CCTCTCCCAG TCTGAAGATA TATCCAGTCT TGGCACAGAT
GTGATAAATG TGACTGAATA TGCTGAAGAA ATTTATCAGT ACCTTAGGGA AGCTGAAATA
AGGCACAGAC CCAAAGCACA CTACATGAAG AAGCAGCCAG ACATCACGGA AGGCATGCGC
ACGATTCTGG TGGACTGGCT GGTGGAGGTT GGGGAAGAAT ATAAACTTCG AGCAGAGACC
CTGTATCTGG CTGTCAACTT CCTGGACAGG TTCCTTTCAT GTATGTCTGT TCTGAGAGGG
AAACTGCAGC TCGTAGGAAC AGCAGCTATG CTTTTGGCTT CGAAATATGA AGAGATATAT
CCTCCTGAAG TAGACGAGTT TGTCTATATC ACCGATGATA CATACACAAA ACGACAACTG
TTAAAAATGG AACACTTGCT TCTGAAAGTT CTAGCTTTTG ATCTGACAGT ACCAACCACC
AACCAGTTTC TCCTTCAGTA CTTGAGGCGA CAAGGAGTGT GCGTCAGGAC TGAGAACCTG
GCTAAGTACG TAGCAGAGCT GAGTCTACTT GAAGCAGATC CATTCTTGAA ATATCTTCCT
TCACTGATAG CTGCAGCAGC TTTTTGCCTG GCAAACTATA CTGTGAACAA GCACTTTTGG
CCAGAAACCC TTGCTGCATT TACAGGGTAT TCATTAAGTG AAATTGTGCC TTGCCTGAGT
GAGCTTCATA AAGCGTACCT TGATATACCC CATCGACCTC AGCAAGCAAT TAGGGAGAAG
TACAAGGCTT CAAAGTACCT GTGTGTGTCC CTCATGGAGC CACCTGCAGT TCTTCTTCTA
CAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25352
Clone ID Related Accession (Same CDS sequence) XM_011535296.2 , XM_011535294.1 , XM_011535297.1 , XM_011535296.1 , NM_001111046.1 , NM_001111047.1 , XM_011535294.2 , XM_011535295.2 , XM_011535295.1
Accession Version XM_011535295.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1266bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-03-12
Organism Homo sapiens(Human)
Product cyclin-A1 isoform X1
Comment

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGCACTGCA GCAACCCCAA GAGTGGAGTT GTGCTGGCTA CAGTGGCCCG AGGTCCCGAT 
GCTTGTCAGA TACTCACCAG AGCCCCGCTG GGCCAGGATC CCCCGCAGAG GACAGTGCTA
GGGCTGCTAA CTGCAAATGG GCAGTACAGG AGGACCTGTG GCCAGGGGAT CACAAGAATC
AGGTGTTATT CTGGATCAGA AAATGCCTTC CCTCCAGCTG GAAAGAAAGC ACTCCCTGAC
TGTGGGGTCC AAGAGCCCCC CAAGCAAGGG TTTGACATCT ACATGGATGA ACTAGAGCAG
GGGGACAGAG ACAGCTGCTC GGTCAGAGAG GGGATGGCAT TTGAGGATGT GTATGAAGTA
GACACCGGCA CACTCAAGTC AGACCTGCAC TTCCTGCTGG ATTTCAACAC AGTTTCCCCT
ATGCTGGTAG ATTCATCTCT CCTCTCCCAG TCTGAAGATA TATCCAGTCT TGGCACAGAT
GTGATAAATG TGACTGAATA TGCTGAAGAA ATTTATCAGT ACCTTAGGGA AGCTGAAATA
AGGCACAGAC CCAAAGCACA CTACATGAAG AAGCAGCCAG ACATCACGGA AGGCATGCGC
ACGATTCTGG TGGACTGGCT GGTGGAGGTT GGGGAAGAAT ATAAACTTCG AGCAGAGACC
CTGTATCTGG CTGTCAACTT CCTGGACAGG TTCCTTTCAT GTATGTCTGT TCTGAGAGGG
AAACTGCAGC TCGTAGGAAC AGCAGCTATG CTTTTGGCTT CGAAATATGA AGAGATATAT
CCTCCTGAAG TAGACGAGTT TGTCTATATC ACCGATGATA CATACACAAA ACGACAACTG
TTAAAAATGG AACACTTGCT TCTGAAAGTT CTAGCTTTTG ATCTGACAGT ACCAACCACC
AACCAGTTTC TCCTTCAGTA CTTGAGGCGA CAAGGAGTGT GCGTCAGGAC TGAGAACCTG
GCTAAGTACG TAGCAGAGCT GAGTCTACTT GAAGCAGATC CATTCTTGAA ATATCTTCCT
TCACTGATAG CTGCAGCAGC TTTTTGCCTG GCAAACTATA CTGTGAACAA GCACTTTTGG
CCAGAAACCC TTGCTGCATT TACAGGGTAT TCATTAAGTG AAATTGTGCC TTGCCTGAGT
GAGCTTCATA AAGCGTACCT TGATATACCC CATCGACCTC AGCAAGCAAT TAGGGAGAAG
TACAAGGCTT CAAAGTACCT GTGTGTGTCC CTCATGGAGC CACCTGCAGT TCTTCTTCTA
CAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011533597.1
Translation

Target ORF information:

RefSeq Version XM_011535295.1
Organism Homo sapiens(Human)
Definition Homo sapiens cyclin A1 (CCNA1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011535295.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGCACTGCA GCAACCCCAA GAGTGGAGTT GTGCTGGCTA CAGTGGCCCG AGGTCCCGAT 
GCTTGTCAGA TACTCACCAG AGCCCCGCTG GGCCAGGATC CCCCGCAGAG GACAGTGCTA
GGGCTGCTAA CTGCAAATGG GCAGTACAGG AGGACCTGTG GCCAGGGGAT CACAAGAATC
AGGTGTTATT CTGGATCAGA AAATGCCTTC CCTCCAGCTG GAAAGAAAGC ACTCCCTGAC
TGTGGGGTCC AAGAGCCCCC CAAGCAAGGG TTTGACATCT ACATGGATGA ACTAGAGCAG
GGGGACAGAG ACAGCTGCTC GGTCAGAGAG GGGATGGCAT TTGAGGATGT GTATGAAGTA
GACACCGGCA CACTCAAGTC AGACCTGCAC TTCCTGCTGG ATTTCAACAC AGTTTCCCCT
ATGCTGGTAG ATTCATCTCT CCTCTCCCAG TCTGAAGATA TATCCAGTCT TGGCACAGAT
GTGATAAATG TGACTGAATA TGCTGAAGAA ATTTATCAGT ACCTTAGGGA AGCTGAAATA
AGGCACAGAC CCAAAGCACA CTACATGAAG AAGCAGCCAG ACATCACGGA AGGCATGCGC
ACGATTCTGG TGGACTGGCT GGTGGAGGTT GGGGAAGAAT ATAAACTTCG AGCAGAGACC
CTGTATCTGG CTGTCAACTT CCTGGACAGG TTCCTTTCAT GTATGTCTGT TCTGAGAGGG
AAACTGCAGC TCGTAGGAAC AGCAGCTATG CTTTTGGCTT CGAAATATGA AGAGATATAT
CCTCCTGAAG TAGACGAGTT TGTCTATATC ACCGATGATA CATACACAAA ACGACAACTG
TTAAAAATGG AACACTTGCT TCTGAAAGTT CTAGCTTTTG ATCTGACAGT ACCAACCACC
AACCAGTTTC TCCTTCAGTA CTTGAGGCGA CAAGGAGTGT GCGTCAGGAC TGAGAACCTG
GCTAAGTACG TAGCAGAGCT GAGTCTACTT GAAGCAGATC CATTCTTGAA ATATCTTCCT
TCACTGATAG CTGCAGCAGC TTTTTGCCTG GCAAACTATA CTGTGAACAA GCACTTTTGG
CCAGAAACCC TTGCTGCATT TACAGGGTAT TCATTAAGTG AAATTGTGCC TTGCCTGAGT
GAGCTTCATA AAGCGTACCT TGATATACCC CATCGACCTC AGCAAGCAAT TAGGGAGAAG
TACAAGGCTT CAAAGTACCT GTGTGTGTCC CTCATGGAGC CACCTGCAGT TCTTCTTCTA
CAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25389
Clone ID Related Accession (Same CDS sequence) NM_003914.3
Accession Version NM_003914.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1398bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-10-06
Organism Homo sapiens(Human)
Product cyclin-A1 isoform a
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC396283.1, DC411883.1, U66838.1 and AA553454.1. On Nov 30, 2007 this sequence version replaced NM_003914.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK314261.1, U66838.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2144333 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGAGACCG GCTTTCCCGC AATCATGTAC CCTGGATCTT TTATTGGGGG CTGGGGAGAA 
GAGTATCTCA GCTGGGAAGG ACCGGGGCTC CCAGATTTCG TCTTCCAGCA GCAGCCCGTG
GAGTCTGAAG CAATGCACTG CAGCAACCCC AAGAGTGGAG TTGTGCTGGC TACAGTGGCC
CGAGGTCCCG ATGCTTGTCA GATACTCACC AGAGCCCCGC TGGGCCAGGA TCCCCCGCAG
AGGACAGTGC TAGGGCTGCT AACTGCAAAT GGGCAGTACA GGAGGACCTG TGGCCAGGGG
ATCACAAGAA TCAGGTGTTA TTCTGGATCA GAAAATGCCT TCCCTCCAGC TGGAAAGAAA
GCACTCCCTG ACTGTGGGGT CCAAGAGCCC CCCAAGCAAG GGTTTGACAT CTACATGGAT
GAACTAGAGC AGGGGGACAG AGACAGCTGC TCGGTCAGAG AGGGGATGGC ATTTGAGGAT
GTGTATGAAG TAGACACCGG CACACTCAAG TCAGACCTGC ACTTCCTGCT GGATTTCAAC
ACAGTTTCCC CTATGCTGGT AGATTCATCT CTCCTCTCCC AGTCTGAAGA TATATCCAGT
CTTGGCACAG ATGTGATAAA TGTGACTGAA TATGCTGAAG AAATTTATCA GTACCTTAGG
GAAGCTGAAA TAAGGCACAG ACCCAAAGCA CACTACATGA AGAAGCAGCC AGACATCACG
GAAGGCATGC GCACGATTCT GGTGGACTGG CTGGTGGAGG TTGGGGAAGA ATATAAACTT
CGAGCAGAGA CCCTGTATCT GGCTGTCAAC TTCCTGGACA GGTTCCTTTC ATGTATGTCT
GTTCTGAGAG GGAAACTGCA GCTCGTAGGA ACAGCAGCTA TGCTTTTGGC TTCGAAATAT
GAAGAGATAT ATCCTCCTGA AGTAGACGAG TTTGTCTATA TCACCGATGA TACATACACA
AAACGACAAC TGTTAAAAAT GGAACACTTG CTTCTGAAAG TTCTAGCTTT TGATCTGACA
GTACCAACCA CCAACCAGTT TCTCCTTCAG TACTTGAGGC GACAAGGAGT GTGCGTCAGG
ACTGAGAACC TGGCTAAGTA CGTAGCAGAG CTGAGTCTAC TTGAAGCAGA TCCATTCTTG
AAATATCTTC CTTCACTGAT AGCTGCAGCA GCTTTTTGCC TGGCAAACTA TACTGTGAAC
AAGCACTTTT GGCCAGAAAC CCTTGCTGCA TTTACAGGGT ATTCATTAAG TGAAATTGTG
CCTTGCCTGA GTGAGCTTCA TAAAGCGTAC CTTGATATAC CCCATCGACC TCAGCAAGCA
ATTAGGGAGA AGTACAAGGC TTCAAAGTAC CTGTGTGTGT CCCTCATGGA GCCACCTGCA
GTTCTTCTTC TACAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_003905.1
CDS351..1748
Misc Feature(1)240..242
Misc Feature(2)561..>785
Misc Feature(3)990..1370
Misc Feature(4)order(1077..1082,1098..1103,1107..1112,1176..1178,1206..1211)
Misc Feature(5)order(1233..1235,1245..1247,1269..1271,1320..1322,1329..1334)
Misc Feature(6)order(1368..1373,1389..1394,1398..1403,1467..1469,1500..1505)
Misc Feature(7)1374..1727
Misc Feature(8)order(1527..1529,1539..1541,1560..1562,1605..1607,1614..1619)
Exon (1)1..458
Exon (2)459..647
Exon (3)648..894
Exon (4)895..1019
Exon (5)1020..1243
Exon (6)1244..1448
Exon (7)1449..1562
Exon (8)1563..1696
Exon (9)1697..1965
Translation

Target ORF information:

RefSeq Version NM_003914.3
Organism Homo sapiens(Human)
Definition Homo sapiens cyclin A1 (CCNA1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_003914.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGAGACCG GCTTTCCCGC AATCATGTAC CCTGGATCTT TTATTGGGGG CTGGGGAGAA 
GAGTATCTCA GCTGGGAAGG ACCGGGGCTC CCAGATTTCG TCTTCCAGCA GCAGCCCGTG
GAGTCTGAAG CAATGCACTG CAGCAACCCC AAGAGTGGAG TTGTGCTGGC TACAGTGGCC
CGAGGTCCCG ATGCTTGTCA GATACTCACC AGAGCCCCGC TGGGCCAGGA TCCCCCGCAG
AGGACAGTGC TAGGGCTGCT AACTGCAAAT GGGCAGTACA GGAGGACCTG TGGCCAGGGG
ATCACAAGAA TCAGGTGTTA TTCTGGATCA GAAAATGCCT TCCCTCCAGC TGGAAAGAAA
GCACTCCCTG ACTGTGGGGT CCAAGAGCCC CCCAAGCAAG GGTTTGACAT CTACATGGAT
GAACTAGAGC AGGGGGACAG AGACAGCTGC TCGGTCAGAG AGGGGATGGC ATTTGAGGAT
GTGTATGAAG TAGACACCGG CACACTCAAG TCAGACCTGC ACTTCCTGCT GGATTTCAAC
ACAGTTTCCC CTATGCTGGT AGATTCATCT CTCCTCTCCC AGTCTGAAGA TATATCCAGT
CTTGGCACAG ATGTGATAAA TGTGACTGAA TATGCTGAAG AAATTTATCA GTACCTTAGG
GAAGCTGAAA TAAGGCACAG ACCCAAAGCA CACTACATGA AGAAGCAGCC AGACATCACG
GAAGGCATGC GCACGATTCT GGTGGACTGG CTGGTGGAGG TTGGGGAAGA ATATAAACTT
CGAGCAGAGA CCCTGTATCT GGCTGTCAAC TTCCTGGACA GGTTCCTTTC ATGTATGTCT
GTTCTGAGAG GGAAACTGCA GCTCGTAGGA ACAGCAGCTA TGCTTTTGGC TTCGAAATAT
GAAGAGATAT ATCCTCCTGA AGTAGACGAG TTTGTCTATA TCACCGATGA TACATACACA
AAACGACAAC TGTTAAAAAT GGAACACTTG CTTCTGAAAG TTCTAGCTTT TGATCTGACA
GTACCAACCA CCAACCAGTT TCTCCTTCAG TACTTGAGGC GACAAGGAGT GTGCGTCAGG
ACTGAGAACC TGGCTAAGTA CGTAGCAGAG CTGAGTCTAC TTGAAGCAGA TCCATTCTTG
AAATATCTTC CTTCACTGAT AGCTGCAGCA GCTTTTTGCC TGGCAAACTA TACTGTGAAC
AAGCACTTTT GGCCAGAAAC CCTTGCTGCA TTTACAGGGT ATTCATTAAG TGAAATTGTG
CCTTGCCTGA GTGAGCTTCA TAAAGCGTAC CTTGATATAC CCCATCGACC TCAGCAAGCA
ATTAGGGAGA AGTACAAGGC TTCAAAGTAC CTGTGTGTGT CCCTCATGGA GCCACCTGCA
GTTCTTCTTC TACAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Human papillomavirus type 16 E7 oncoprotein mediates CCNA1 promoter methylation.
Cancer science106(10)1333-40(2015 Oct)
Chalertpet K,Pakdeechaidan W,Patel V,Mutirangura A,Yanatatsaneejit P


book

Correlation of CCNA1 promoter methylation with malignant tumors: a meta-analysis introduction.
BioMed research international2015134027(2015)
Yang B,Miao S,Zhang LN,Sun HB,Xu ZN,Han CS


book

DNA methylation status of key cell-cycle regulators such as CDKNA2/p16 and CCNA1 correlates with treatment response to doxorubicin and 5-fluorouracil in locally advanced breast tumors.
Clinical cancer research : an official journal of the American Association for Cancer Research20(24)6357-66(2014 Dec)
Klajic J,Busato F,Edvardsen H,Touleimat N,Fleischer T,Bukholm I,Børresen-Dale AL,Lønning PE,Tost J,Kristensen VN


book

Elucidating the roles of miR-372 in cell proliferation and apoptosis of nasopharyngeal carcinoma TW01 cells.
Experimental oncology36(3)170-3(2014 Sep)
Tan JK,Tan EL,Gan SY


book

CCNA1 promoter methylation: a potential marker for grading Papanicolaou smear cervical squamous intraepithelial lesions.
Asian Pacific journal of cancer prevention : APJCP15(18)7971-5(2014)
Chujan S,Kitkumthorn N,Siriangkul S,Mutirangura A


 
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