CD3D cDNA ORF clone, Homo sapiens(human)
| Gene Symbol | CD3D |
| Entrez Gene ID | 915 |
| Full Name | CD3d molecule |
| Synonyms | CD3-DELTA,IMD19,T3D |
| General protein information |
|
| Gene Type | protein-coding |
| Organism | Homo sapiens(human) |
| Genome | |
| Summary | The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined. [provided by RefSeq, Feb 2009]. |
| Disorder MIM: | |
| Disorder Html: | Immunodeficiency 19, 615617 (3) |
mRNA and Protein(s)
| mRNA | Protein | Name |
|---|---|---|
| NM_001040651.2 | NP_001035741.1 | T-cell surface glycoprotein CD3 delta chain isoform B precursor |
| NM_001040651.1 | NP_001035741.1 | T-cell surface glycoprotein CD3 delta chain isoform B precursor |
| Homo sapiens (human) | CD3D | NP_000723.1 |
| CD3D | XP_001097302.1 | |
| Xenopus tropicalis (tropical clawed frog) | cd3g | NP_001135555.1 |
| Bos taurus (cattle) | CD3D | NP_001029205.1 |
| Rattus norvegicus (Norway rat) | Cd3d | NP_037301.1 |
| Mus musculus (house mouse) | Cd3d | NP_038515.3 |
| Gallus gallus (chicken) | CD3D | NP_990843.1 |
| Pan troglodytes (chimpanzee) | CD3D | XP_508789.2 |
| Canis lupus familiaris (dog) | CD3D | XP_536556.1 |
Related articles in PubMed
Balanced secretion of anti-CEA ? anti-CD3 diabody chains using the 2A self-cleaving peptide maximizes diabody assembly and tumor-specific cytotoxicity.
M?lgaard K, Compte M, Nu?ez-Prado N, Harwood SL, Sanz L, Alvarez-Vallina L
Gene therapy24(4)208-214(2017 Apr)
CD3D and PRKCQ work together to discriminate between B-cell and T-cell acute lymphoblastic leukemia.
Ma D, Zhong S, Liu X, Mai H, Mai G, Xu C, Zhou F
Computers in biology and medicine7716-22(2016 10)
Identification of a FOXP3(+)CD3(+)CD56(+) population with immunosuppressive function in cancer tissues of human hepatocellular carcinoma.
Li X, Peng J, Pang Y, Yu S, Yu X, Chen P, Wang W, Han W, Zhang J, Yin Y, Zhang Y
Scientific reports514757(2015 Oct)
Identification of the Docking Site for CD3 on the T Cell Receptor ? Chain by Solution NMR.
He Y, Rangarajan S, Kerzic M, Luo M, Chen Y, Wang Q, Yin Y, Workman CJ, Vignali KM, Vignali DA, Mariuzza RA, Orban J
The Journal of biological chemistry290(32)19796-805(2015 Aug)
Molecular architecture of the ?? T cell receptor-CD3 complex.
Birnbaum ME, Berry R, Hsiao YS, Chen Z, Shingu-Vazquez MA, Yu X, Waghray D, Fischer S, McCluskey J, Rossjohn J, Walz T, Garcia KC
Proceedings of the National Academy of Sciences of the United States of America111(49)17576-81(2014 Dec)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
we demonstrate that the incorporation of a 2A self-processing peptide derived from foot-and-mouth disease virus conveying co-translational cleavage into a two-chain anti-CD3 x anti-CEA diabody gene enables near-equimolar expression of diabody chains 1 and 2, and thus increases the final amount of assembled diabody.
Title: Balanced secretion of anti-CEA ? anti-CD3 diabody chains using the 2A self-cleaving peptide maximizes diabody assembly and tumor-specific cytotoxicity.
PCR and transcriptome analysis suggest that the genes CD3D and PKRCQ together can be used as a model for differentiating between B-cell and T-cell acute lymphoblastic leukemia.
Title: CD3D and PRKCQ work together to discriminate between B-cell and T-cell acute lymphoblastic leukemia.
A FOXP3(+)CD3(+)CD56(+)-expressed T-cell population with immunosuppressive function and reduced patient survival has been identified in cancer tissues of human hepatocellular carcinoma.
Title: Identification of a FOXP3(+)CD3(+)CD56(+) population with immunosuppressive function in cancer tissues of human hepatocellular carcinoma.
The docking site for CD3 subunits on the T Cell receptor beta chain has been identified by solution NMR.
Title: Identification of the Docking Site for CD3 on the T Cell Receptor ? Chain by Solution NMR.
analysis of the molecular organization of the TCR-CD3 complex
Title: Molecular architecture of the ?? T cell receptor-CD3 complex.
The following CD3D gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CD3D cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OHu05021 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001040651.2 , NM_001040651.1 | |
| Accession Version | NM_001040651.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 384bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-09-28 | |
| Organism | Homo sapiens(human) | |
| Product | T-cell surface glycoprotein CD3 delta chain isoform B precursor | |
| Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC070321.1, CD014058.1 and AP001582.4. On Jun 2, 2019 this sequence version replaced NM_001040651.1. Transcript Variant: This variant (2) lacks an exon in the coding region, compared to variant 1. The encoded protein (B) is shorter and lacks the transmembrane domain, compared to isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CD014058.1, AI219691.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2146236, SAMEA2150385 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length. | |
1 | ATGGAACATA GCACGTTTCT CTCTGGCCTG GTACTGGCTA CCCTTCTCTC GCAAGTGAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001035741.1 |
| CDS | 99..482 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001040651.2 |
ORF Insert Sequence:
1 | ATGGAACATA GCACGTTTCT CTCTGGCCTG GTACTGGCTA CCCTTCTCTC GCAAGTGAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu05021 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001040651.2 , NM_001040651.1 | |
| Accession Version | NM_001040651.1 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 384bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-06-22 | |
| Organism | Homo sapiens(human) | |
| Product | T-cell surface glycoprotein CD3 delta chain isoform B precursor | |
| Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC070321.1 and CD014058.1. Transcript Variant: This variant (2) lacks an exon in the coding region, compared to variant 1. The encoded protein (B) is shorter and lacks the transmembrane domain, compared to isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CD014058.1, AI219691.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2146236, SAMEA2150385 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length. | |
1 | ATGGAACATA GCACGTTTCT CTCTGGCCTG GTACTGGCTA CCCTTCTCTC GCAAGTGAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001035741.1 |
| CDS | 138..521 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001040651.1 |
ORF Insert Sequence:
1 | ATGGAACATA GCACGTTTCT CTCTGGCCTG GTACTGGCTA CCCTTCTCTC GCAAGTGAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
 |
Balanced secretion of anti-CEA ? anti-CD3 diabody chains using the 2A self-cleaving peptide maximizes diabody assembly and tumor-specific cytotoxicity. |
|
CD3D and PRKCQ work together to discriminate between B-cell and T-cell acute lymphoblastic leukemia. |
|
Identification of a FOXP3(+)CD3(+)CD56(+) population with immunosuppressive function in cancer tissues of human hepatocellular carcinoma. |
|
Identification of the Docking Site for CD3 on the T Cell Receptor ? Chain by Solution NMR. |
|
Molecular architecture of the ?? T cell receptor-CD3 complex. |