ROCK2 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_017005378.2	XP_016860867.1	rho-associated protein kinase 2 isoform X2
NM_004850.3	NP_004841.2	rho-associated protein kinase 2
XM_017005379.1	XP_016860868.1	rho-associated protein kinase 2 isoform X3
XM_011510417.2	XP_011508719.1	rho-associated protein kinase 2 isoform X3
NM_004850.4	NP_004841.2	rho-associated protein kinase 2 isoform 1
XM_017005379.2	XP_016860868.1	rho-associated protein kinase 2 isoform X3
NM_004850.5	NP_004841.2	rho-associated protein kinase 2 isoform 1
NM_001321643.1	NP_001308572.1	rho-associated protein kinase 2 isoform 2
XM_017005378.1	XP_016860867.1	rho-associated protein kinase 2 isoform X2
NM_001321643.2	NP_001308572.1	rho-associated protein kinase 2 isoform 2
XM_005246190.3	XP_005246247.1	rho-associated protein kinase 2 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26833	NM_004850.5 Latest version!	Homo sapiens Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu102041	NM_001321643.2 Latest version!	Homo sapiens Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$643.30 $919.00
OHu62859	XM_011510417.2 Latest version!	Homo sapiens Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$643.30 $919.00
OHu62859	XM_017005379.2 Latest version!	Homo sapiens Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$643.30 $919.00
OHu35229	XM_005246190.3 Latest version!	Homo sapiens Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu92799	XM_017005378.2 Latest version!	Homo sapiens Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
View Old Accession Versions >>
OHu26833	NM_004850.3	Homo sapiens Rho-associated, coiled-coil containing protein kinase 2 (ROCK2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu62859	XM_017005379.1	Homo sapiens Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$643.30 $919.00
OHu26833	NM_004850.4	Homo sapiens Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu92799	XM_017005378.1	Homo sapiens Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu102041	NM_001321643.1	Homo sapiens Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$643.30 $919.00
Hide Old Accession Versions >>

ROCK2 ( NM_004850.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004841.2 Homo sapiens Rho-associated, coiled-coil containing protein kinase 2 (ROCK2), mRNA.

Price & Availability↑

CloneID	OHu26833
Clone ID Related Accession (Same CDS sequence)	NM_004850.3 , NM_004850.4 , NM_004850.5
Accession Version	NM_004850.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	rho-associated protein kinase 2
Comment

ATGAGCCGGC CCCCGCCGAC GGGGAAAATG CCCGGCGCCC CCGAGACCGC GCCGGGGGAC 
GGGGCAGGCG CGAGCCGCCA GAGGAAGCTG GAGGCGCTGA TCCGAGACCC TCGCTCCCCC 
ATCAACGTGG AGAGCTTGCT GGATGGCTTA AATTCCTTGG TCCTTGATTT AGATTTTCCT 
GCTTTGAGGA AAAACAAGAA CATAGATAAT TTCTTAAATA GATATGAGAA AATTGTGAAA 
AAAATCAGAG GTCTACAGAT GAAGGCAGAA GACTATGATG TTGTAAAAGT TATTGGAAGA 
GGTGCTTTTG GTGAAGTGCA GTTGGTTCGT CACAAGGCAT CGCAGAAGGT TTATGCTATG 
AAGCTTCTTA GTAAGTTTGA AATGATAAAA AGATCAGATT CTGCCTTTTT TTGGGAAGAA 
AGAGATATTA TGGCCTTTGC CAATAGCCCC TGGGTGGTTC AGCTTTTTTA TGCCTTTCAA 
GATGATAGGT ATCTGTACAT GGTAATGGAG TACATGCCTG GTGGAGACCT TGTAAACCTT 
ATGAGTAATT ATGATGTGCC TGAAAAATGG GCCAAATTTT ACACTGCTGA AGTTGTTCTT 
GCTCTGGATG CAATACACTC CATGGGTTTA ATACACAGAG ATGTGAAGCC TGACAACATG 
CTCTTGGATA AACATGGACA TCTAAAATTA GCAGATTTTG GCACGTGTAT GAAGATGGAT 
GAAACAGGCA TGGTACATTG TGATACAGCA GTTGGAACAC CGGATTATAT ATCACCTGAG 
GTTCTGAAAT CACAAGGGGG TGATGGTTTC TATGGGCGAG AATGTGATTG GTGGTCTGTA 
GGTGTTTTCC TTTATGAGAT GCTAGTGGGG GATACTCCAT TTTATGCGGA TTCACTTGTA 
GGAACATATA GCAAAATTAT GGATCATAAG AATTCACTGT GTTTCCCTGA AGATGCAGAA 
ATTTCCAAAC ATGCAAAGAA TCTCATCTGT GCTTTCTTAA CAGATAGGGA GGTACGACTT 
GGGAGAAATG GGGTGGAAGA AATCAGACAG CATCCTTTCT TTAAGAATGA TCAGTGGCAT 
TGGGATAACA TAAGAGAAAC GGCAGCTCCT GTAGTACCTG AACTCAGCAG TGACATAGAC 
AGCAGCAATT TCGATGACAT TGAAGATGAC AAAGGAGATG TAGAAACCTT CCCAATTCCT 
AAAGCTTTTG TTGGAAATCA GCTGCCTTTC ATCGGATTTA CCTACTATAG AGAAAATTTA 
TTATTAAGTG ACTCTCCATC TTGTAGAGAA ACTGATTCCA TACAATCAAG GAAAAATGAA 
GAAAGTCAAG AGATTCAGAA AAAACTGTAT ACATTAGAAG AACATCTTAG CAATGAGATG 
CAAGCCAAAG AGGAACTGGA ACAGAAGTGC AAATCTGTTA ATACTCGCCT AGAAAAAACA 
GCAAAGGAGC TAGAAGAGGA GATTACCTTA CGGAAAAGTG TGGAATCAGC ATTAAGACAG 
TTAGAAAGAG AAAAGGCGCT TCTTCAGCAC AAAAATGCAG AATATCAGAG GAAAGCTGAT 
CATGAAGCAG ACAAAAAACG AAATTTGGAA AATGATGTTA ACAGCTTAAA AGATCAACTT 
GAAGATTTGA AAAAAAGAAA TCAAAACTCT CAAATATCCA CTGAGAAAGT GAATCAACTC 
CAGAGACAAC TGGATGAAAC CAATGCTTTA CTGCGAACAG AGTCTGATAC TGCAGCCCGG 
TTAAGGAAAA CCCAGGCAGA AAGTTCAAAA CAGATTCAGC AGCTGGAATC TAACAATAGA 
GATCTACAAG ATAAAAACTG CCTGCTGGAG ACTGCCAAGT TAAAACTTGA AAAGGAATTT 
ATCAATCTTC AGTCAGCTCT AGAATCTGAA AGGAGGGATC GAACCCATGG ATCAGAGATA 
ATTAATGATT TACAAGGTAG AATATGTGGC CTAGAAGAAG ATTTAAAGAA CGGCAAAATC 
TTACTAGCGA AAGTAGAACT GGAGAAGAGA CAACTTCAGG AGAGATTTAC TGATTTGGAA 
AAGGAAAAAA GCAACATGGA AATAGATATG ACATACCAAC TAAAAGTTAT ACAGCAGAGC 
CTAGAACAAG AAGAAGCTGA ACATAAGGCC ACAAAGGCAC GACTAGCAGA TAAAAATAAG 
ATCTATGAGT CCATCGAAGA AGCCAAATCA GAAGCCATGA AAGAAATGGA GAAGAAGCTC 
TTGGAGGAAA GAACTTTAAA ACAGAAAGTG GAGAACCTAT TGCTAGAAGC TGAGAAAAGA 
TGTTCTCTAT TAGACTGTGA CCTCAAACAG TCACAGCAGA AAATAAATGA GCTCCTTAAA 
CAGAAAGATG TGCTAAATGA GGATGTTAGA AACCTGACAT TAAAAATAGA GCAAGAAACT 
CAGAAGCGCT GCCTTACACA AAATGACCTG AAGATGCAAA CACAACAGGT TAACACACTA 
AAAATGTCAG AAAAGCAGTT AAAGCAAGAA AATAACCATC TCATGGAAAT GAAAATGAAC 
TTGGAAAAAC AAAATGCTGA ACTTCGAAAA GAACGTCAGG ATGCAGATGG GCAAATGAAA 
GAGCTCCAGG ATCAGCTCGA AGCAGAACAG TATTTCTCAA CCCTTTATAA AACACAAGTT 
AGGGAGCTTA AAGAAGAATG TGAAGAAAAG ACCAAACTTG GTAAAGAATT GCAGCAGAAG 
AAACAGGAAT TACAGGATGA ACGGGACTCT TTGGCTGCCC AACTGGAGAT CACCTTGACC 
AAAGCAGATT CTGAGCAACT GGCTCGTTCA ATTGCTGAAG AACAATATTC TGATTTGGAA 
AAAGAGAAGA TCATGAAAGA GCTGGAGATC AAAGAGATGA TGGCTAGACA CAAACAGGAA 
CTTACGGAAA AAGATGCTAC AATTGCTTCT CTTGAGGAAA CTAATAGGAC ACTAACTAGT 
GATGTTGCCA ATCTTGCAAA TGAGAAAGAA GAATTAAATA ACAAATTGAA AGATGTTCAA 
GAGCAACTGT CAAGATTGAA AGATGAAGAA ATAAGCGCAG CAGCTATTAA AGCACAGTTT 
GAGAAGCAGC TATTAACAGA AAGAACACTC AAAACTCAAG CTGTGAATAA GTTGGCTGAG 
ATCATGAATC GAAAAGAACC TGTCAAGCGT GGTAATGACA CAGATGTGCG GAGAAAAGAG 
AAGGAGAATA GAAAGCTACA TATGGAGCTT AAATCTGAAC GTGAGAAATT GACCCAGCAG 
ATGATCAAGT ATCAGAAAGA ACTGAATGAA ATGCAGGCAC AAATAGCTGA AGAGAGCCAG 
ATTCGAATTG AACTGCAGAT GACATTGGAC AGTAAAGACA GTGACATTGA GCAGCTGCGG 
TCACAACTCC AAGCCTTGCA TATTGGTCTG GATAGTTCCA GTATAGGCAG TGGACCAGGG 
GATGCTGAGG CAGATGATGG GTTTCCAGAA TCAAGATTAG AAGGATGGCT TTCATTGCCT 
GTACGAAACA ACACTAAGAA ATTTGGATGG GTTAAAAAGT ATGTGATTGT AAGCAGTAAG 
AAGATTCTTT TCTATGACAG TGAACAAGAT AAAGAACAAT CCAATCCTTA CATGGTTTTA 
GATATAGACA AGTTATTTCA TGTCCGACCA GTTACACAGA CAGATGTGTA TAGAGCAGAT 
GCTAAAGAAA TTCCAAGGAT ATTCCAGATT CTGTATGCCA ATGAAGGAGA AAGTAAGAAG 
GAACAAGAAT TTCCAGTGGA GCCAGTTGGA GAAAAATCTA ATTATATTTG CCACAAGGGA 
CATGAGTTTA TTCCTACTCT TTATCATTTC CCAACCAACT GTGAGGCTTG TATGAAGCCC 
CTGTGGCACA TGTTTAAGCC TCCTCCTGCT TTGGAGTGCC GCCGTTGCCA TATTAAGTGT 
CATAAAGATC ATATGGACAA AAAGGAGGAG ATTATAGCAC CTTGCAAAGT ATATTATGAT 
ATTTCAACGG CAAAGAATCT GTTATTACTA GCAAATTCTA CAGAAGAGCA GCAGAAGTGG 
GTTAGTCGGT TGGTGAAAAA GATACCTAAA AAGCCCCCAG CTCCAGACCC TTTTGCCCGA 
TCATCTCCTA GAACTTCAAT GAAGATACAG CAAAACCAGT CTATTAGACG GCCAAGTCGA 
CAGCTTGCCC CAAACAAACC TAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_004841.2

Translation

MSRPPPTGKMPGAPETAPGDGAGASRQRKLEALIRDPRSPINVESLLDGLNSLVLDLDFPALRKNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGFYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDAEISKHAKNLICAFLTDREVRLGRNGVEEIRQHPFFKNDQWHWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYRENLLLSDSPSCRETDSIQSRKNEESQEIQKKLYTLEEHLSNEMQAKEELEQKCKSVNTRLEKTAKELEEEITLRKSVESALRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQNSQISTEKVNQLQRQLDETNALLRTESDTAARLRKTQAESSKQIQQLESNNRDLQDKNCLLETAKLKLEKEFINLQSALESERRDRTHGSEIINDLQGRICGLEEDLKNGKILLAKVELEKRQLQERFTDLEKEKSNMEIDMTYQLKVIQQSLEQEEAEHKATKARLADKNKIYESIEEAKSEAMKEMEKKLLEERTLKQKVENLLLEAEKRCSLLDCDLKQSQQKINELLKQKDVLNEDVRNLTLKIEQETQKRCLTQNDLKMQTQQVNTLKMSEKQLKQENNHLMEMKMNLEKQNAELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEECEEKTKLGKELQQKKQELQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELTEKDATIASLEETNRTLTSDVANLANEKEELNNKLKDVQEQLSRLKDEEISAAAIKAQFEKQLLTERTLKTQAVNKLAEIMNRKEPVKRGNDTDVRRKEKENRKLHMELKSEREKLTQQMIKYQKELNEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQALHIGLDSSSIGSGPGDAEADDGFPESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEQEFPVEPVGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISTAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPS

Target ORF information:

RefSeq Version	NM_004850.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens Rho-associated, coiled-coil containing protein kinase 2 (ROCK2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004850.3

ORF Insert Sequence:

ATGAGCCGGC CCCCGCCGAC GGGGAAAATG CCCGGCGCCC CCGAGACCGC GCCGGGGGAC 
GGGGCAGGCG CGAGCCGCCA GAGGAAGCTG GAGGCGCTGA TCCGAGACCC TCGCTCCCCC 
ATCAACGTGG AGAGCTTGCT GGATGGCTTA AATTCCTTGG TCCTTGATTT AGATTTTCCT 
GCTTTGAGGA AAAACAAGAA CATAGATAAT TTCTTAAATA GATATGAGAA AATTGTGAAA 
AAAATCAGAG GTCTACAGAT GAAGGCAGAA GACTATGATG TTGTAAAAGT TATTGGAAGA 
GGTGCTTTTG GTGAAGTGCA GTTGGTTCGT CACAAGGCAT CGCAGAAGGT TTATGCTATG 
AAGCTTCTTA GTAAGTTTGA AATGATAAAA AGATCAGATT CTGCCTTTTT TTGGGAAGAA 
AGAGATATTA TGGCCTTTGC CAATAGCCCC TGGGTGGTTC AGCTTTTTTA TGCCTTTCAA 
GATGATAGGT ATCTGTACAT GGTAATGGAG TACATGCCTG GTGGAGACCT TGTAAACCTT 
ATGAGTAATT ATGATGTGCC TGAAAAATGG GCCAAATTTT ACACTGCTGA AGTTGTTCTT 
GCTCTGGATG CAATACACTC CATGGGTTTA ATACACAGAG ATGTGAAGCC TGACAACATG 
CTCTTGGATA AACATGGACA TCTAAAATTA GCAGATTTTG GCACGTGTAT GAAGATGGAT 
GAAACAGGCA TGGTACATTG TGATACAGCA GTTGGAACAC CGGATTATAT ATCACCTGAG 
GTTCTGAAAT CACAAGGGGG TGATGGTTTC TATGGGCGAG AATGTGATTG GTGGTCTGTA 
GGTGTTTTCC TTTATGAGAT GCTAGTGGGG GATACTCCAT TTTATGCGGA TTCACTTGTA 
GGAACATATA GCAAAATTAT GGATCATAAG AATTCACTGT GTTTCCCTGA AGATGCAGAA 
ATTTCCAAAC ATGCAAAGAA TCTCATCTGT GCTTTCTTAA CAGATAGGGA GGTACGACTT 
GGGAGAAATG GGGTGGAAGA AATCAGACAG CATCCTTTCT TTAAGAATGA TCAGTGGCAT 
TGGGATAACA TAAGAGAAAC GGCAGCTCCT GTAGTACCTG AACTCAGCAG TGACATAGAC 
AGCAGCAATT TCGATGACAT TGAAGATGAC AAAGGAGATG TAGAAACCTT CCCAATTCCT 
AAAGCTTTTG TTGGAAATCA GCTGCCTTTC ATCGGATTTA CCTACTATAG AGAAAATTTA 
TTATTAAGTG ACTCTCCATC TTGTAGAGAA ACTGATTCCA TACAATCAAG GAAAAATGAA 
GAAAGTCAAG AGATTCAGAA AAAACTGTAT ACATTAGAAG AACATCTTAG CAATGAGATG 
CAAGCCAAAG AGGAACTGGA ACAGAAGTGC AAATCTGTTA ATACTCGCCT AGAAAAAACA 
GCAAAGGAGC TAGAAGAGGA GATTACCTTA CGGAAAAGTG TGGAATCAGC ATTAAGACAG 
TTAGAAAGAG AAAAGGCGCT TCTTCAGCAC AAAAATGCAG AATATCAGAG GAAAGCTGAT 
CATGAAGCAG ACAAAAAACG AAATTTGGAA AATGATGTTA ACAGCTTAAA AGATCAACTT 
GAAGATTTGA AAAAAAGAAA TCAAAACTCT CAAATATCCA CTGAGAAAGT GAATCAACTC 
CAGAGACAAC TGGATGAAAC CAATGCTTTA CTGCGAACAG AGTCTGATAC TGCAGCCCGG 
TTAAGGAAAA CCCAGGCAGA AAGTTCAAAA CAGATTCAGC AGCTGGAATC TAACAATAGA 
GATCTACAAG ATAAAAACTG CCTGCTGGAG ACTGCCAAGT TAAAACTTGA AAAGGAATTT 
ATCAATCTTC AGTCAGCTCT AGAATCTGAA AGGAGGGATC GAACCCATGG ATCAGAGATA 
ATTAATGATT TACAAGGTAG AATATGTGGC CTAGAAGAAG ATTTAAAGAA CGGCAAAATC 
TTACTAGCGA AAGTAGAACT GGAGAAGAGA CAACTTCAGG AGAGATTTAC TGATTTGGAA 
AAGGAAAAAA GCAACATGGA AATAGATATG ACATACCAAC TAAAAGTTAT ACAGCAGAGC 
CTAGAACAAG AAGAAGCTGA ACATAAGGCC ACAAAGGCAC GACTAGCAGA TAAAAATAAG 
ATCTATGAGT CCATCGAAGA AGCCAAATCA GAAGCCATGA AAGAAATGGA GAAGAAGCTC 
TTGGAGGAAA GAACTTTAAA ACAGAAAGTG GAGAACCTAT TGCTAGAAGC TGAGAAAAGA 
TGTTCTCTAT TAGACTGTGA CCTCAAACAG TCACAGCAGA AAATAAATGA GCTCCTTAAA 
CAGAAAGATG TGCTAAATGA GGATGTTAGA AACCTGACAT TAAAAATAGA GCAAGAAACT 
CAGAAGCGCT GCCTTACACA AAATGACCTG AAGATGCAAA CACAACAGGT TAACACACTA 
AAAATGTCAG AAAAGCAGTT AAAGCAAGAA AATAACCATC TCATGGAAAT GAAAATGAAC 
TTGGAAAAAC AAAATGCTGA ACTTCGAAAA GAACGTCAGG ATGCAGATGG GCAAATGAAA 
GAGCTCCAGG ATCAGCTCGA AGCAGAACAG TATTTCTCAA CCCTTTATAA AACACAAGTT 
AGGGAGCTTA AAGAAGAATG TGAAGAAAAG ACCAAACTTG GTAAAGAATT GCAGCAGAAG 
AAACAGGAAT TACAGGATGA ACGGGACTCT TTGGCTGCCC AACTGGAGAT CACCTTGACC 
AAAGCAGATT CTGAGCAACT GGCTCGTTCA ATTGCTGAAG AACAATATTC TGATTTGGAA 
AAAGAGAAGA TCATGAAAGA GCTGGAGATC AAAGAGATGA TGGCTAGACA CAAACAGGAA 
CTTACGGAAA AAGATGCTAC AATTGCTTCT CTTGAGGAAA CTAATAGGAC ACTAACTAGT 
GATGTTGCCA ATCTTGCAAA TGAGAAAGAA GAATTAAATA ACAAATTGAA AGATGTTCAA 
GAGCAACTGT CAAGATTGAA AGATGAAGAA ATAAGCGCAG CAGCTATTAA AGCACAGTTT 
GAGAAGCAGC TATTAACAGA AAGAACACTC AAAACTCAAG CTGTGAATAA GTTGGCTGAG 
ATCATGAATC GAAAAGAACC TGTCAAGCGT GGTAATGACA CAGATGTGCG GAGAAAAGAG 
AAGGAGAATA GAAAGCTACA TATGGAGCTT AAATCTGAAC GTGAGAAATT GACCCAGCAG 
ATGATCAAGT ATCAGAAAGA ACTGAATGAA ATGCAGGCAC AAATAGCTGA AGAGAGCCAG 
ATTCGAATTG AACTGCAGAT GACATTGGAC AGTAAAGACA GTGACATTGA GCAGCTGCGG 
TCACAACTCC AAGCCTTGCA TATTGGTCTG GATAGTTCCA GTATAGGCAG TGGACCAGGG 
GATGCTGAGG CAGATGATGG GTTTCCAGAA TCAAGATTAG AAGGATGGCT TTCATTGCCT 
GTACGAAACA ACACTAAGAA ATTTGGATGG GTTAAAAAGT ATGTGATTGT AAGCAGTAAG 
AAGATTCTTT TCTATGACAG TGAACAAGAT AAAGAACAAT CCAATCCTTA CATGGTTTTA 
GATATAGACA AGTTATTTCA TGTCCGACCA GTTACACAGA CAGATGTGTA TAGAGCAGAT 
GCTAAAGAAA TTCCAAGGAT ATTCCAGATT CTGTATGCCA ATGAAGGAGA AAGTAAGAAG 
GAACAAGAAT TTCCAGTGGA GCCAGTTGGA GAAAAATCTA ATTATATTTG CCACAAGGGA 
CATGAGTTTA TTCCTACTCT TTATCATTTC CCAACCAACT GTGAGGCTTG TATGAAGCCC 
CTGTGGCACA TGTTTAAGCC TCCTCCTGCT TTGGAGTGCC GCCGTTGCCA TATTAAGTGT 
CATAAAGATC ATATGGACAA AAAGGAGGAG ATTATAGCAC CTTGCAAAGT ATATTATGAT 
ATTTCAACGG CAAAGAATCT GTTATTACTA GCAAATTCTA CAGAAGAGCA GCAGAAGTGG 
GTTAGTCGGT TGGTGAAAAA GATACCTAAA AAGCCCCCAG CTCCAGACCC TTTTGCCCGA 
TCATCTCCTA GAACTTCAAT GAAGATACAG CAAAACCAGT CTATTAGACG GCCAAGTCGA 
CAGCTTGCCC CAAACAAACC TAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROCK2 ( NM_004850.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004841.2 Homo sapiens Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26833
Clone ID Related Accession (Same CDS sequence)	NM_004850.3 , NM_004850.4 , NM_004850.5
Accession Version	NM_004850.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-10
Organism	Homo sapiens(human)
Product	rho-associated protein kinase 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB014519.1, AC018463.11, BC111801.1 and AL049383.1. This sequence is a reference standard in the RefSeqGene project. On Mar 29, 2016 this sequence version replaced NM_004850.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB014519.1, SRR1803614.259314.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCCGGC CCCCGCCGAC GGGGAAAATG CCCGGCGCCC CCGAGACCGC GCCGGGGGAC 
GGGGCAGGCG CGAGCCGCCA GAGGAAGCTG GAGGCGCTGA TCCGAGACCC TCGCTCCCCC 
ATCAACGTGG AGAGCTTGCT GGATGGCTTA AATTCCTTGG TCCTTGATTT AGATTTTCCT 
GCTTTGAGGA AAAACAAGAA CATAGATAAT TTCTTAAATA GATATGAGAA AATTGTGAAA 
AAAATCAGAG GTCTACAGAT GAAGGCAGAA GACTATGATG TTGTAAAAGT TATTGGAAGA 
GGTGCTTTTG GTGAAGTGCA GTTGGTTCGT CACAAGGCAT CGCAGAAGGT TTATGCTATG 
AAGCTTCTTA GTAAGTTTGA AATGATAAAA AGATCAGATT CTGCCTTTTT TTGGGAAGAA 
AGAGATATTA TGGCCTTTGC CAATAGCCCC TGGGTGGTTC AGCTTTTTTA TGCCTTTCAA 
GATGATAGGT ATCTGTACAT GGTAATGGAG TACATGCCTG GTGGAGACCT TGTAAACCTT 
ATGAGTAATT ATGATGTGCC TGAAAAATGG GCCAAATTTT ACACTGCTGA AGTTGTTCTT 
GCTCTGGATG CAATACACTC CATGGGTTTA ATACACAGAG ATGTGAAGCC TGACAACATG 
CTCTTGGATA AACATGGACA TCTAAAATTA GCAGATTTTG GCACGTGTAT GAAGATGGAT 
GAAACAGGCA TGGTACATTG TGATACAGCA GTTGGAACAC CGGATTATAT ATCACCTGAG 
GTTCTGAAAT CACAAGGGGG TGATGGTTTC TATGGGCGAG AATGTGATTG GTGGTCTGTA 
GGTGTTTTCC TTTATGAGAT GCTAGTGGGG GATACTCCAT TTTATGCGGA TTCACTTGTA 
GGAACATATA GCAAAATTAT GGATCATAAG AATTCACTGT GTTTCCCTGA AGATGCAGAA 
ATTTCCAAAC ATGCAAAGAA TCTCATCTGT GCTTTCTTAA CAGATAGGGA GGTACGACTT 
GGGAGAAATG GGGTGGAAGA AATCAGACAG CATCCTTTCT TTAAGAATGA TCAGTGGCAT 
TGGGATAACA TAAGAGAAAC GGCAGCTCCT GTAGTACCTG AACTCAGCAG TGACATAGAC 
AGCAGCAATT TCGATGACAT TGAAGATGAC AAAGGAGATG TAGAAACCTT CCCAATTCCT 
AAAGCTTTTG TTGGAAATCA GCTGCCTTTC ATCGGATTTA CCTACTATAG AGAAAATTTA 
TTATTAAGTG ACTCTCCATC TTGTAGAGAA ACTGATTCCA TACAATCAAG GAAAAATGAA 
GAAAGTCAAG AGATTCAGAA AAAACTGTAT ACATTAGAAG AACATCTTAG CAATGAGATG 
CAAGCCAAAG AGGAACTGGA ACAGAAGTGC AAATCTGTTA ATACTCGCCT AGAAAAAACA 
GCAAAGGAGC TAGAAGAGGA GATTACCTTA CGGAAAAGTG TGGAATCAGC ATTAAGACAG 
TTAGAAAGAG AAAAGGCGCT TCTTCAGCAC AAAAATGCAG AATATCAGAG GAAAGCTGAT 
CATGAAGCAG ACAAAAAACG AAATTTGGAA AATGATGTTA ACAGCTTAAA AGATCAACTT 
GAAGATTTGA AAAAAAGAAA TCAAAACTCT CAAATATCCA CTGAGAAAGT GAATCAACTC 
CAGAGACAAC TGGATGAAAC CAATGCTTTA CTGCGAACAG AGTCTGATAC TGCAGCCCGG 
TTAAGGAAAA CCCAGGCAGA AAGTTCAAAA CAGATTCAGC AGCTGGAATC TAACAATAGA 
GATCTACAAG ATAAAAACTG CCTGCTGGAG ACTGCCAAGT TAAAACTTGA AAAGGAATTT 
ATCAATCTTC AGTCAGCTCT AGAATCTGAA AGGAGGGATC GAACCCATGG ATCAGAGATA 
ATTAATGATT TACAAGGTAG AATATGTGGC CTAGAAGAAG ATTTAAAGAA CGGCAAAATC 
TTACTAGCGA AAGTAGAACT GGAGAAGAGA CAACTTCAGG AGAGATTTAC TGATTTGGAA 
AAGGAAAAAA GCAACATGGA AATAGATATG ACATACCAAC TAAAAGTTAT ACAGCAGAGC 
CTAGAACAAG AAGAAGCTGA ACATAAGGCC ACAAAGGCAC GACTAGCAGA TAAAAATAAG 
ATCTATGAGT CCATCGAAGA AGCCAAATCA GAAGCCATGA AAGAAATGGA GAAGAAGCTC 
TTGGAGGAAA GAACTTTAAA ACAGAAAGTG GAGAACCTAT TGCTAGAAGC TGAGAAAAGA 
TGTTCTCTAT TAGACTGTGA CCTCAAACAG TCACAGCAGA AAATAAATGA GCTCCTTAAA 
CAGAAAGATG TGCTAAATGA GGATGTTAGA AACCTGACAT TAAAAATAGA GCAAGAAACT 
CAGAAGCGCT GCCTTACACA AAATGACCTG AAGATGCAAA CACAACAGGT TAACACACTA 
AAAATGTCAG AAAAGCAGTT AAAGCAAGAA AATAACCATC TCATGGAAAT GAAAATGAAC 
TTGGAAAAAC AAAATGCTGA ACTTCGAAAA GAACGTCAGG ATGCAGATGG GCAAATGAAA 
GAGCTCCAGG ATCAGCTCGA AGCAGAACAG TATTTCTCAA CCCTTTATAA AACACAAGTT 
AGGGAGCTTA AAGAAGAATG TGAAGAAAAG ACCAAACTTG GTAAAGAATT GCAGCAGAAG 
AAACAGGAAT TACAGGATGA ACGGGACTCT TTGGCTGCCC AACTGGAGAT CACCTTGACC 
AAAGCAGATT CTGAGCAACT GGCTCGTTCA ATTGCTGAAG AACAATATTC TGATTTGGAA 
AAAGAGAAGA TCATGAAAGA GCTGGAGATC AAAGAGATGA TGGCTAGACA CAAACAGGAA 
CTTACGGAAA AAGATGCTAC AATTGCTTCT CTTGAGGAAA CTAATAGGAC ACTAACTAGT 
GATGTTGCCA ATCTTGCAAA TGAGAAAGAA GAATTAAATA ACAAATTGAA AGATGTTCAA 
GAGCAACTGT CAAGATTGAA AGATGAAGAA ATAAGCGCAG CAGCTATTAA AGCACAGTTT 
GAGAAGCAGC TATTAACAGA AAGAACACTC AAAACTCAAG CTGTGAATAA GTTGGCTGAG 
ATCATGAATC GAAAAGAACC TGTCAAGCGT GGTAATGACA CAGATGTGCG GAGAAAAGAG 
AAGGAGAATA GAAAGCTACA TATGGAGCTT AAATCTGAAC GTGAGAAATT GACCCAGCAG 
ATGATCAAGT ATCAGAAAGA ACTGAATGAA ATGCAGGCAC AAATAGCTGA AGAGAGCCAG 
ATTCGAATTG AACTGCAGAT GACATTGGAC AGTAAAGACA GTGACATTGA GCAGCTGCGG 
TCACAACTCC AAGCCTTGCA TATTGGTCTG GATAGTTCCA GTATAGGCAG TGGACCAGGG 
GATGCTGAGG CAGATGATGG GTTTCCAGAA TCAAGATTAG AAGGATGGCT TTCATTGCCT 
GTACGAAACA ACACTAAGAA ATTTGGATGG GTTAAAAAGT ATGTGATTGT AAGCAGTAAG 
AAGATTCTTT TCTATGACAG TGAACAAGAT AAAGAACAAT CCAATCCTTA CATGGTTTTA 
GATATAGACA AGTTATTTCA TGTCCGACCA GTTACACAGA CAGATGTGTA TAGAGCAGAT 
GCTAAAGAAA TTCCAAGGAT ATTCCAGATT CTGTATGCCA ATGAAGGAGA AAGTAAGAAG 
GAACAAGAAT TTCCAGTGGA GCCAGTTGGA GAAAAATCTA ATTATATTTG CCACAAGGGA 
CATGAGTTTA TTCCTACTCT TTATCATTTC CCAACCAACT GTGAGGCTTG TATGAAGCCC 
CTGTGGCACA TGTTTAAGCC TCCTCCTGCT TTGGAGTGCC GCCGTTGCCA TATTAAGTGT 
CATAAAGATC ATATGGACAA AAAGGAGGAG ATTATAGCAC CTTGCAAAGT ATATTATGAT 
ATTTCAACGG CAAAGAATCT GTTATTACTA GCAAATTCTA CAGAAGAGCA GCAGAAGTGG 
GTTAGTCGGT TGGTGAAAAA GATACCTAAA AAGCCCCCAG CTCCAGACCC TTTTGCCCGA 
TCATCTCCTA GAACTTCAAT GAAGATACAG CAAAACCAGT CTATTAGACG GCCAAGTCGA 
CAGCTTGCCC CAAACAAACC TAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004841.2
CDS	450..4616
Translation	MSRPPPTGKMPGAPETAPGDGAGASRQRKLEALIRDPRSPINVESLLDGLNSLVLDLDFPALRKNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGFYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDAEISKHAKNLICAFLTDREVRLGRNGVEEIRQHPFFKNDQWHWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYRENLLLSDSPSCRETDSIQSRKNEESQEIQKKLYTLEEHLSNEMQAKEELEQKCKSVNTRLEKTAKELEEEITLRKSVESALRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQNSQISTEKVNQLQRQLDETNALLRTESDTAARLRKTQAESSKQIQQLESNNRDLQDKNCLLETAKLKLEKEFINLQSALESERRDRTHGSEIINDLQGRICGLEEDLKNGKILLAKVELEKRQLQERFTDLEKEKSNMEIDMTYQLKVIQQSLEQEEAEHKATKARLADKNKIYESIEEAKSEAMKEMEKKLLEERTLKQKVENLLLEAEKRCSLLDCDLKQSQQKINELLKQKDVLNEDVRNLTLKIEQETQKRCLTQNDLKMQTQQVNTLKMSEKQLKQENNHLMEMKMNLEKQNAELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEECEEKTKLGKELQQKKQELQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELTEKDATIASLEETNRTLTSDVANLANEKEELNNKLKDVQEQLSRLKDEEISAAAIKAQFEKQLLTERTLKTQAVNKLAEIMNRKEPVKRGNDTDVRRKEKENRKLHMELKSEREKLTQQMIKYQKELNEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQALHIGLDSSSIGSGPGDAEADDGFPESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEQEFPVEPVGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISTAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPS

Target ORF information:

RefSeq Version	NM_004850.4
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004850.4

ORF Insert Sequence:

ATGAGCCGGC CCCCGCCGAC GGGGAAAATG CCCGGCGCCC CCGAGACCGC GCCGGGGGAC 
GGGGCAGGCG CGAGCCGCCA GAGGAAGCTG GAGGCGCTGA TCCGAGACCC TCGCTCCCCC 
ATCAACGTGG AGAGCTTGCT GGATGGCTTA AATTCCTTGG TCCTTGATTT AGATTTTCCT 
GCTTTGAGGA AAAACAAGAA CATAGATAAT TTCTTAAATA GATATGAGAA AATTGTGAAA 
AAAATCAGAG GTCTACAGAT GAAGGCAGAA GACTATGATG TTGTAAAAGT TATTGGAAGA 
GGTGCTTTTG GTGAAGTGCA GTTGGTTCGT CACAAGGCAT CGCAGAAGGT TTATGCTATG 
AAGCTTCTTA GTAAGTTTGA AATGATAAAA AGATCAGATT CTGCCTTTTT TTGGGAAGAA 
AGAGATATTA TGGCCTTTGC CAATAGCCCC TGGGTGGTTC AGCTTTTTTA TGCCTTTCAA 
GATGATAGGT ATCTGTACAT GGTAATGGAG TACATGCCTG GTGGAGACCT TGTAAACCTT 
ATGAGTAATT ATGATGTGCC TGAAAAATGG GCCAAATTTT ACACTGCTGA AGTTGTTCTT 
GCTCTGGATG CAATACACTC CATGGGTTTA ATACACAGAG ATGTGAAGCC TGACAACATG 
CTCTTGGATA AACATGGACA TCTAAAATTA GCAGATTTTG GCACGTGTAT GAAGATGGAT 
GAAACAGGCA TGGTACATTG TGATACAGCA GTTGGAACAC CGGATTATAT ATCACCTGAG 
GTTCTGAAAT CACAAGGGGG TGATGGTTTC TATGGGCGAG AATGTGATTG GTGGTCTGTA 
GGTGTTTTCC TTTATGAGAT GCTAGTGGGG GATACTCCAT TTTATGCGGA TTCACTTGTA 
GGAACATATA GCAAAATTAT GGATCATAAG AATTCACTGT GTTTCCCTGA AGATGCAGAA 
ATTTCCAAAC ATGCAAAGAA TCTCATCTGT GCTTTCTTAA CAGATAGGGA GGTACGACTT 
GGGAGAAATG GGGTGGAAGA AATCAGACAG CATCCTTTCT TTAAGAATGA TCAGTGGCAT 
TGGGATAACA TAAGAGAAAC GGCAGCTCCT GTAGTACCTG AACTCAGCAG TGACATAGAC 
AGCAGCAATT TCGATGACAT TGAAGATGAC AAAGGAGATG TAGAAACCTT CCCAATTCCT 
AAAGCTTTTG TTGGAAATCA GCTGCCTTTC ATCGGATTTA CCTACTATAG AGAAAATTTA 
TTATTAAGTG ACTCTCCATC TTGTAGAGAA ACTGATTCCA TACAATCAAG GAAAAATGAA 
GAAAGTCAAG AGATTCAGAA AAAACTGTAT ACATTAGAAG AACATCTTAG CAATGAGATG 
CAAGCCAAAG AGGAACTGGA ACAGAAGTGC AAATCTGTTA ATACTCGCCT AGAAAAAACA 
GCAAAGGAGC TAGAAGAGGA GATTACCTTA CGGAAAAGTG TGGAATCAGC ATTAAGACAG 
TTAGAAAGAG AAAAGGCGCT TCTTCAGCAC AAAAATGCAG AATATCAGAG GAAAGCTGAT 
CATGAAGCAG ACAAAAAACG AAATTTGGAA AATGATGTTA ACAGCTTAAA AGATCAACTT 
GAAGATTTGA AAAAAAGAAA TCAAAACTCT CAAATATCCA CTGAGAAAGT GAATCAACTC 
CAGAGACAAC TGGATGAAAC CAATGCTTTA CTGCGAACAG AGTCTGATAC TGCAGCCCGG 
TTAAGGAAAA CCCAGGCAGA AAGTTCAAAA CAGATTCAGC AGCTGGAATC TAACAATAGA 
GATCTACAAG ATAAAAACTG CCTGCTGGAG ACTGCCAAGT TAAAACTTGA AAAGGAATTT 
ATCAATCTTC AGTCAGCTCT AGAATCTGAA AGGAGGGATC GAACCCATGG ATCAGAGATA 
ATTAATGATT TACAAGGTAG AATATGTGGC CTAGAAGAAG ATTTAAAGAA CGGCAAAATC 
TTACTAGCGA AAGTAGAACT GGAGAAGAGA CAACTTCAGG AGAGATTTAC TGATTTGGAA 
AAGGAAAAAA GCAACATGGA AATAGATATG ACATACCAAC TAAAAGTTAT ACAGCAGAGC 
CTAGAACAAG AAGAAGCTGA ACATAAGGCC ACAAAGGCAC GACTAGCAGA TAAAAATAAG 
ATCTATGAGT CCATCGAAGA AGCCAAATCA GAAGCCATGA AAGAAATGGA GAAGAAGCTC 
TTGGAGGAAA GAACTTTAAA ACAGAAAGTG GAGAACCTAT TGCTAGAAGC TGAGAAAAGA 
TGTTCTCTAT TAGACTGTGA CCTCAAACAG TCACAGCAGA AAATAAATGA GCTCCTTAAA 
CAGAAAGATG TGCTAAATGA GGATGTTAGA AACCTGACAT TAAAAATAGA GCAAGAAACT 
CAGAAGCGCT GCCTTACACA AAATGACCTG AAGATGCAAA CACAACAGGT TAACACACTA 
AAAATGTCAG AAAAGCAGTT AAAGCAAGAA AATAACCATC TCATGGAAAT GAAAATGAAC 
TTGGAAAAAC AAAATGCTGA ACTTCGAAAA GAACGTCAGG ATGCAGATGG GCAAATGAAA 
GAGCTCCAGG ATCAGCTCGA AGCAGAACAG TATTTCTCAA CCCTTTATAA AACACAAGTT 
AGGGAGCTTA AAGAAGAATG TGAAGAAAAG ACCAAACTTG GTAAAGAATT GCAGCAGAAG 
AAACAGGAAT TACAGGATGA ACGGGACTCT TTGGCTGCCC AACTGGAGAT CACCTTGACC 
AAAGCAGATT CTGAGCAACT GGCTCGTTCA ATTGCTGAAG AACAATATTC TGATTTGGAA 
AAAGAGAAGA TCATGAAAGA GCTGGAGATC AAAGAGATGA TGGCTAGACA CAAACAGGAA 
CTTACGGAAA AAGATGCTAC AATTGCTTCT CTTGAGGAAA CTAATAGGAC ACTAACTAGT 
GATGTTGCCA ATCTTGCAAA TGAGAAAGAA GAATTAAATA ACAAATTGAA AGATGTTCAA 
GAGCAACTGT CAAGATTGAA AGATGAAGAA ATAAGCGCAG CAGCTATTAA AGCACAGTTT 
GAGAAGCAGC TATTAACAGA AAGAACACTC AAAACTCAAG CTGTGAATAA GTTGGCTGAG 
ATCATGAATC GAAAAGAACC TGTCAAGCGT GGTAATGACA CAGATGTGCG GAGAAAAGAG 
AAGGAGAATA GAAAGCTACA TATGGAGCTT AAATCTGAAC GTGAGAAATT GACCCAGCAG 
ATGATCAAGT ATCAGAAAGA ACTGAATGAA ATGCAGGCAC AAATAGCTGA AGAGAGCCAG 
ATTCGAATTG AACTGCAGAT GACATTGGAC AGTAAAGACA GTGACATTGA GCAGCTGCGG 
TCACAACTCC AAGCCTTGCA TATTGGTCTG GATAGTTCCA GTATAGGCAG TGGACCAGGG 
GATGCTGAGG CAGATGATGG GTTTCCAGAA TCAAGATTAG AAGGATGGCT TTCATTGCCT 
GTACGAAACA ACACTAAGAA ATTTGGATGG GTTAAAAAGT ATGTGATTGT AAGCAGTAAG 
AAGATTCTTT TCTATGACAG TGAACAAGAT AAAGAACAAT CCAATCCTTA CATGGTTTTA 
GATATAGACA AGTTATTTCA TGTCCGACCA GTTACACAGA CAGATGTGTA TAGAGCAGAT 
GCTAAAGAAA TTCCAAGGAT ATTCCAGATT CTGTATGCCA ATGAAGGAGA AAGTAAGAAG 
GAACAAGAAT TTCCAGTGGA GCCAGTTGGA GAAAAATCTA ATTATATTTG CCACAAGGGA 
CATGAGTTTA TTCCTACTCT TTATCATTTC CCAACCAACT GTGAGGCTTG TATGAAGCCC 
CTGTGGCACA TGTTTAAGCC TCCTCCTGCT TTGGAGTGCC GCCGTTGCCA TATTAAGTGT 
CATAAAGATC ATATGGACAA AAAGGAGGAG ATTATAGCAC CTTGCAAAGT ATATTATGAT 
ATTTCAACGG CAAAGAATCT GTTATTACTA GCAAATTCTA CAGAAGAGCA GCAGAAGTGG 
GTTAGTCGGT TGGTGAAAAA GATACCTAAA AAGCCCCCAG CTCCAGACCC TTTTGCCCGA 
TCATCTCCTA GAACTTCAAT GAAGATACAG CAAAACCAGT CTATTAGACG GCCAAGTCGA 
CAGCTTGCCC CAAACAAACC TAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROCK2 ( NM_004850.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_004841.2 Homo sapiens Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant 1, mRNA.

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CloneID	OHu26833
Clone ID Related Accession (Same CDS sequence)	NM_004850.3 , NM_004850.4 , NM_004850.5
Accession Version	NM_004850.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	rho-associated protein kinase 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC099344.4, AB014519.1, AC018463.11, BC111801.1 and AL049383.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_004850.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB014519.1, SRR1803614.259314.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000315872.11/ ENSP00000317985.6 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGCCGGC CCCCGCCGAC GGGGAAAATG CCCGGCGCCC CCGAGACCGC GCCGGGGGAC 
GGGGCAGGCG CGAGCCGCCA GAGGAAGCTG GAGGCGCTGA TCCGAGACCC TCGCTCCCCC 
ATCAACGTGG AGAGCTTGCT GGATGGCTTA AATTCCTTGG TCCTTGATTT AGATTTTCCT 
GCTTTGAGGA AAAACAAGAA CATAGATAAT TTCTTAAATA GATATGAGAA AATTGTGAAA 
AAAATCAGAG GTCTACAGAT GAAGGCAGAA GACTATGATG TTGTAAAAGT TATTGGAAGA 
GGTGCTTTTG GTGAAGTGCA GTTGGTTCGT CACAAGGCAT CGCAGAAGGT TTATGCTATG 
AAGCTTCTTA GTAAGTTTGA AATGATAAAA AGATCAGATT CTGCCTTTTT TTGGGAAGAA 
AGAGATATTA TGGCCTTTGC CAATAGCCCC TGGGTGGTTC AGCTTTTTTA TGCCTTTCAA 
GATGATAGGT ATCTGTACAT GGTAATGGAG TACATGCCTG GTGGAGACCT TGTAAACCTT 
ATGAGTAATT ATGATGTGCC TGAAAAATGG GCCAAATTTT ACACTGCTGA AGTTGTTCTT 
GCTCTGGATG CAATACACTC CATGGGTTTA ATACACAGAG ATGTGAAGCC TGACAACATG 
CTCTTGGATA AACATGGACA TCTAAAATTA GCAGATTTTG GCACGTGTAT GAAGATGGAT 
GAAACAGGCA TGGTACATTG TGATACAGCA GTTGGAACAC CGGATTATAT ATCACCTGAG 
GTTCTGAAAT CACAAGGGGG TGATGGTTTC TATGGGCGAG AATGTGATTG GTGGTCTGTA 
GGTGTTTTCC TTTATGAGAT GCTAGTGGGG GATACTCCAT TTTATGCGGA TTCACTTGTA 
GGAACATATA GCAAAATTAT GGATCATAAG AATTCACTGT GTTTCCCTGA AGATGCAGAA 
ATTTCCAAAC ATGCAAAGAA TCTCATCTGT GCTTTCTTAA CAGATAGGGA GGTACGACTT 
GGGAGAAATG GGGTGGAAGA AATCAGACAG CATCCTTTCT TTAAGAATGA TCAGTGGCAT 
TGGGATAACA TAAGAGAAAC GGCAGCTCCT GTAGTACCTG AACTCAGCAG TGACATAGAC 
AGCAGCAATT TCGATGACAT TGAAGATGAC AAAGGAGATG TAGAAACCTT CCCAATTCCT 
AAAGCTTTTG TTGGAAATCA GCTGCCTTTC ATCGGATTTA CCTACTATAG AGAAAATTTA 
TTATTAAGTG ACTCTCCATC TTGTAGAGAA ACTGATTCCA TACAATCAAG GAAAAATGAA 
GAAAGTCAAG AGATTCAGAA AAAACTGTAT ACATTAGAAG AACATCTTAG CAATGAGATG 
CAAGCCAAAG AGGAACTGGA ACAGAAGTGC AAATCTGTTA ATACTCGCCT AGAAAAAACA 
GCAAAGGAGC TAGAAGAGGA GATTACCTTA CGGAAAAGTG TGGAATCAGC ATTAAGACAG 
TTAGAAAGAG AAAAGGCGCT TCTTCAGCAC AAAAATGCAG AATATCAGAG GAAAGCTGAT 
CATGAAGCAG ACAAAAAACG AAATTTGGAA AATGATGTTA ACAGCTTAAA AGATCAACTT 
GAAGATTTGA AAAAAAGAAA TCAAAACTCT CAAATATCCA CTGAGAAAGT GAATCAACTC 
CAGAGACAAC TGGATGAAAC CAATGCTTTA CTGCGAACAG AGTCTGATAC TGCAGCCCGG 
TTAAGGAAAA CCCAGGCAGA AAGTTCAAAA CAGATTCAGC AGCTGGAATC TAACAATAGA 
GATCTACAAG ATAAAAACTG CCTGCTGGAG ACTGCCAAGT TAAAACTTGA AAAGGAATTT 
ATCAATCTTC AGTCAGCTCT AGAATCTGAA AGGAGGGATC GAACCCATGG ATCAGAGATA 
ATTAATGATT TACAAGGTAG AATATGTGGC CTAGAAGAAG ATTTAAAGAA CGGCAAAATC 
TTACTAGCGA AAGTAGAACT GGAGAAGAGA CAACTTCAGG AGAGATTTAC TGATTTGGAA 
AAGGAAAAAA GCAACATGGA AATAGATATG ACATACCAAC TAAAAGTTAT ACAGCAGAGC 
CTAGAACAAG AAGAAGCTGA ACATAAGGCC ACAAAGGCAC GACTAGCAGA TAAAAATAAG 
ATCTATGAGT CCATCGAAGA AGCCAAATCA GAAGCCATGA AAGAAATGGA GAAGAAGCTC 
TTGGAGGAAA GAACTTTAAA ACAGAAAGTG GAGAACCTAT TGCTAGAAGC TGAGAAAAGA 
TGTTCTCTAT TAGACTGTGA CCTCAAACAG TCACAGCAGA AAATAAATGA GCTCCTTAAA 
CAGAAAGATG TGCTAAATGA GGATGTTAGA AACCTGACAT TAAAAATAGA GCAAGAAACT 
CAGAAGCGCT GCCTTACACA AAATGACCTG AAGATGCAAA CACAACAGGT TAACACACTA 
AAAATGTCAG AAAAGCAGTT AAAGCAAGAA AATAACCATC TCATGGAAAT GAAAATGAAC 
TTGGAAAAAC AAAATGCTGA ACTTCGAAAA GAACGTCAGG ATGCAGATGG GCAAATGAAA 
GAGCTCCAGG ATCAGCTCGA AGCAGAACAG TATTTCTCAA CCCTTTATAA AACACAAGTT 
AGGGAGCTTA AAGAAGAATG TGAAGAAAAG ACCAAACTTG GTAAAGAATT GCAGCAGAAG 
AAACAGGAAT TACAGGATGA ACGGGACTCT TTGGCTGCCC AACTGGAGAT CACCTTGACC 
AAAGCAGATT CTGAGCAACT GGCTCGTTCA ATTGCTGAAG AACAATATTC TGATTTGGAA 
AAAGAGAAGA TCATGAAAGA GCTGGAGATC AAAGAGATGA TGGCTAGACA CAAACAGGAA 
CTTACGGAAA AAGATGCTAC AATTGCTTCT CTTGAGGAAA CTAATAGGAC ACTAACTAGT 
GATGTTGCCA ATCTTGCAAA TGAGAAAGAA GAATTAAATA ACAAATTGAA AGATGTTCAA 
GAGCAACTGT CAAGATTGAA AGATGAAGAA ATAAGCGCAG CAGCTATTAA AGCACAGTTT 
GAGAAGCAGC TATTAACAGA AAGAACACTC AAAACTCAAG CTGTGAATAA GTTGGCTGAG 
ATCATGAATC GAAAAGAACC TGTCAAGCGT GGTAATGACA CAGATGTGCG GAGAAAAGAG 
AAGGAGAATA GAAAGCTACA TATGGAGCTT AAATCTGAAC GTGAGAAATT GACCCAGCAG 
ATGATCAAGT ATCAGAAAGA ACTGAATGAA ATGCAGGCAC AAATAGCTGA AGAGAGCCAG 
ATTCGAATTG AACTGCAGAT GACATTGGAC AGTAAAGACA GTGACATTGA GCAGCTGCGG 
TCACAACTCC AAGCCTTGCA TATTGGTCTG GATAGTTCCA GTATAGGCAG TGGACCAGGG 
GATGCTGAGG CAGATGATGG GTTTCCAGAA TCAAGATTAG AAGGATGGCT TTCATTGCCT 
GTACGAAACA ACACTAAGAA ATTTGGATGG GTTAAAAAGT ATGTGATTGT AAGCAGTAAG 
AAGATTCTTT TCTATGACAG TGAACAAGAT AAAGAACAAT CCAATCCTTA CATGGTTTTA 
GATATAGACA AGTTATTTCA TGTCCGACCA GTTACACAGA CAGATGTGTA TAGAGCAGAT 
GCTAAAGAAA TTCCAAGGAT ATTCCAGATT CTGTATGCCA ATGAAGGAGA AAGTAAGAAG 
GAACAAGAAT TTCCAGTGGA GCCAGTTGGA GAAAAATCTA ATTATATTTG CCACAAGGGA 
CATGAGTTTA TTCCTACTCT TTATCATTTC CCAACCAACT GTGAGGCTTG TATGAAGCCC 
CTGTGGCACA TGTTTAAGCC TCCTCCTGCT TTGGAGTGCC GCCGTTGCCA TATTAAGTGT 
CATAAAGATC ATATGGACAA AAAGGAGGAG ATTATAGCAC CTTGCAAAGT ATATTATGAT 
ATTTCAACGG CAAAGAATCT GTTATTACTA GCAAATTCTA CAGAAGAGCA GCAGAAGTGG 
GTTAGTCGGT TGGTGAAAAA GATACCTAAA AAGCCCCCAG CTCCAGACCC TTTTGCCCGA 
TCATCTCCTA GAACTTCAAT GAAGATACAG CAAAACCAGT CTATTAGACG GCCAAGTCGA 
CAGCTTGCCC CAAACAAACC TAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004841.2
CDS	501..4667
Translation	MSRPPPTGKMPGAPETAPGDGAGASRQRKLEALIRDPRSPINVESLLDGLNSLVLDLDFPALRKNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGFYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDAEISKHAKNLICAFLTDREVRLGRNGVEEIRQHPFFKNDQWHWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYRENLLLSDSPSCRETDSIQSRKNEESQEIQKKLYTLEEHLSNEMQAKEELEQKCKSVNTRLEKTAKELEEEITLRKSVESALRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQNSQISTEKVNQLQRQLDETNALLRTESDTAARLRKTQAESSKQIQQLESNNRDLQDKNCLLETAKLKLEKEFINLQSALESERRDRTHGSEIINDLQGRICGLEEDLKNGKILLAKVELEKRQLQERFTDLEKEKSNMEIDMTYQLKVIQQSLEQEEAEHKATKARLADKNKIYESIEEAKSEAMKEMEKKLLEERTLKQKVENLLLEAEKRCSLLDCDLKQSQQKINELLKQKDVLNEDVRNLTLKIEQETQKRCLTQNDLKMQTQQVNTLKMSEKQLKQENNHLMEMKMNLEKQNAELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEECEEKTKLGKELQQKKQELQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELTEKDATIASLEETNRTLTSDVANLANEKEELNNKLKDVQEQLSRLKDEEISAAAIKAQFEKQLLTERTLKTQAVNKLAEIMNRKEPVKRGNDTDVRRKEKENRKLHMELKSEREKLTQQMIKYQKELNEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQALHIGLDSSSIGSGPGDAEADDGFPESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEQEFPVEPVGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISTAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPS

Target ORF information:

RefSeq Version	NM_004850.5
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004850.5

ORF Insert Sequence:

ATGAGCCGGC CCCCGCCGAC GGGGAAAATG CCCGGCGCCC CCGAGACCGC GCCGGGGGAC 
GGGGCAGGCG CGAGCCGCCA GAGGAAGCTG GAGGCGCTGA TCCGAGACCC TCGCTCCCCC 
ATCAACGTGG AGAGCTTGCT GGATGGCTTA AATTCCTTGG TCCTTGATTT AGATTTTCCT 
GCTTTGAGGA AAAACAAGAA CATAGATAAT TTCTTAAATA GATATGAGAA AATTGTGAAA 
AAAATCAGAG GTCTACAGAT GAAGGCAGAA GACTATGATG TTGTAAAAGT TATTGGAAGA 
GGTGCTTTTG GTGAAGTGCA GTTGGTTCGT CACAAGGCAT CGCAGAAGGT TTATGCTATG 
AAGCTTCTTA GTAAGTTTGA AATGATAAAA AGATCAGATT CTGCCTTTTT TTGGGAAGAA 
AGAGATATTA TGGCCTTTGC CAATAGCCCC TGGGTGGTTC AGCTTTTTTA TGCCTTTCAA 
GATGATAGGT ATCTGTACAT GGTAATGGAG TACATGCCTG GTGGAGACCT TGTAAACCTT 
ATGAGTAATT ATGATGTGCC TGAAAAATGG GCCAAATTTT ACACTGCTGA AGTTGTTCTT 
GCTCTGGATG CAATACACTC CATGGGTTTA ATACACAGAG ATGTGAAGCC TGACAACATG 
CTCTTGGATA AACATGGACA TCTAAAATTA GCAGATTTTG GCACGTGTAT GAAGATGGAT 
GAAACAGGCA TGGTACATTG TGATACAGCA GTTGGAACAC CGGATTATAT ATCACCTGAG 
GTTCTGAAAT CACAAGGGGG TGATGGTTTC TATGGGCGAG AATGTGATTG GTGGTCTGTA 
GGTGTTTTCC TTTATGAGAT GCTAGTGGGG GATACTCCAT TTTATGCGGA TTCACTTGTA 
GGAACATATA GCAAAATTAT GGATCATAAG AATTCACTGT GTTTCCCTGA AGATGCAGAA 
ATTTCCAAAC ATGCAAAGAA TCTCATCTGT GCTTTCTTAA CAGATAGGGA GGTACGACTT 
GGGAGAAATG GGGTGGAAGA AATCAGACAG CATCCTTTCT TTAAGAATGA TCAGTGGCAT 
TGGGATAACA TAAGAGAAAC GGCAGCTCCT GTAGTACCTG AACTCAGCAG TGACATAGAC 
AGCAGCAATT TCGATGACAT TGAAGATGAC AAAGGAGATG TAGAAACCTT CCCAATTCCT 
AAAGCTTTTG TTGGAAATCA GCTGCCTTTC ATCGGATTTA CCTACTATAG AGAAAATTTA 
TTATTAAGTG ACTCTCCATC TTGTAGAGAA ACTGATTCCA TACAATCAAG GAAAAATGAA 
GAAAGTCAAG AGATTCAGAA AAAACTGTAT ACATTAGAAG AACATCTTAG CAATGAGATG 
CAAGCCAAAG AGGAACTGGA ACAGAAGTGC AAATCTGTTA ATACTCGCCT AGAAAAAACA 
GCAAAGGAGC TAGAAGAGGA GATTACCTTA CGGAAAAGTG TGGAATCAGC ATTAAGACAG 
TTAGAAAGAG AAAAGGCGCT TCTTCAGCAC AAAAATGCAG AATATCAGAG GAAAGCTGAT 
CATGAAGCAG ACAAAAAACG AAATTTGGAA AATGATGTTA ACAGCTTAAA AGATCAACTT 
GAAGATTTGA AAAAAAGAAA TCAAAACTCT CAAATATCCA CTGAGAAAGT GAATCAACTC 
CAGAGACAAC TGGATGAAAC CAATGCTTTA CTGCGAACAG AGTCTGATAC TGCAGCCCGG 
TTAAGGAAAA CCCAGGCAGA AAGTTCAAAA CAGATTCAGC AGCTGGAATC TAACAATAGA 
GATCTACAAG ATAAAAACTG CCTGCTGGAG ACTGCCAAGT TAAAACTTGA AAAGGAATTT 
ATCAATCTTC AGTCAGCTCT AGAATCTGAA AGGAGGGATC GAACCCATGG ATCAGAGATA 
ATTAATGATT TACAAGGTAG AATATGTGGC CTAGAAGAAG ATTTAAAGAA CGGCAAAATC 
TTACTAGCGA AAGTAGAACT GGAGAAGAGA CAACTTCAGG AGAGATTTAC TGATTTGGAA 
AAGGAAAAAA GCAACATGGA AATAGATATG ACATACCAAC TAAAAGTTAT ACAGCAGAGC 
CTAGAACAAG AAGAAGCTGA ACATAAGGCC ACAAAGGCAC GACTAGCAGA TAAAAATAAG 
ATCTATGAGT CCATCGAAGA AGCCAAATCA GAAGCCATGA AAGAAATGGA GAAGAAGCTC 
TTGGAGGAAA GAACTTTAAA ACAGAAAGTG GAGAACCTAT TGCTAGAAGC TGAGAAAAGA 
TGTTCTCTAT TAGACTGTGA CCTCAAACAG TCACAGCAGA AAATAAATGA GCTCCTTAAA 
CAGAAAGATG TGCTAAATGA GGATGTTAGA AACCTGACAT TAAAAATAGA GCAAGAAACT 
CAGAAGCGCT GCCTTACACA AAATGACCTG AAGATGCAAA CACAACAGGT TAACACACTA 
AAAATGTCAG AAAAGCAGTT AAAGCAAGAA AATAACCATC TCATGGAAAT GAAAATGAAC 
TTGGAAAAAC AAAATGCTGA ACTTCGAAAA GAACGTCAGG ATGCAGATGG GCAAATGAAA 
GAGCTCCAGG ATCAGCTCGA AGCAGAACAG TATTTCTCAA CCCTTTATAA AACACAAGTT 
AGGGAGCTTA AAGAAGAATG TGAAGAAAAG ACCAAACTTG GTAAAGAATT GCAGCAGAAG 
AAACAGGAAT TACAGGATGA ACGGGACTCT TTGGCTGCCC AACTGGAGAT CACCTTGACC 
AAAGCAGATT CTGAGCAACT GGCTCGTTCA ATTGCTGAAG AACAATATTC TGATTTGGAA 
AAAGAGAAGA TCATGAAAGA GCTGGAGATC AAAGAGATGA TGGCTAGACA CAAACAGGAA 
CTTACGGAAA AAGATGCTAC AATTGCTTCT CTTGAGGAAA CTAATAGGAC ACTAACTAGT 
GATGTTGCCA ATCTTGCAAA TGAGAAAGAA GAATTAAATA ACAAATTGAA AGATGTTCAA 
GAGCAACTGT CAAGATTGAA AGATGAAGAA ATAAGCGCAG CAGCTATTAA AGCACAGTTT 
GAGAAGCAGC TATTAACAGA AAGAACACTC AAAACTCAAG CTGTGAATAA GTTGGCTGAG 
ATCATGAATC GAAAAGAACC TGTCAAGCGT GGTAATGACA CAGATGTGCG GAGAAAAGAG 
AAGGAGAATA GAAAGCTACA TATGGAGCTT AAATCTGAAC GTGAGAAATT GACCCAGCAG 
ATGATCAAGT ATCAGAAAGA ACTGAATGAA ATGCAGGCAC AAATAGCTGA AGAGAGCCAG 
ATTCGAATTG AACTGCAGAT GACATTGGAC AGTAAAGACA GTGACATTGA GCAGCTGCGG 
TCACAACTCC AAGCCTTGCA TATTGGTCTG GATAGTTCCA GTATAGGCAG TGGACCAGGG 
GATGCTGAGG CAGATGATGG GTTTCCAGAA TCAAGATTAG AAGGATGGCT TTCATTGCCT 
GTACGAAACA ACACTAAGAA ATTTGGATGG GTTAAAAAGT ATGTGATTGT AAGCAGTAAG 
AAGATTCTTT TCTATGACAG TGAACAAGAT AAAGAACAAT CCAATCCTTA CATGGTTTTA 
GATATAGACA AGTTATTTCA TGTCCGACCA GTTACACAGA CAGATGTGTA TAGAGCAGAT 
GCTAAAGAAA TTCCAAGGAT ATTCCAGATT CTGTATGCCA ATGAAGGAGA AAGTAAGAAG 
GAACAAGAAT TTCCAGTGGA GCCAGTTGGA GAAAAATCTA ATTATATTTG CCACAAGGGA 
CATGAGTTTA TTCCTACTCT TTATCATTTC CCAACCAACT GTGAGGCTTG TATGAAGCCC 
CTGTGGCACA TGTTTAAGCC TCCTCCTGCT TTGGAGTGCC GCCGTTGCCA TATTAAGTGT 
CATAAAGATC ATATGGACAA AAAGGAGGAG ATTATAGCAC CTTGCAAAGT ATATTATGAT 
ATTTCAACGG CAAAGAATCT GTTATTACTA GCAAATTCTA CAGAAGAGCA GCAGAAGTGG 
GTTAGTCGGT TGGTGAAAAA GATACCTAAA AAGCCCCCAG CTCCAGACCC TTTTGCCCGA 
TCATCTCCTA GAACTTCAAT GAAGATACAG CAAAACCAGT CTATTAGACG GCCAAGTCGA 
CAGCTTGCCC CAAACAAACC TAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROCK2 ( NM_001321643.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001308572.1 Homo sapiens Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant 2, mRNA.

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CloneID	OHu102041
Clone ID Related Accession (Same CDS sequence)	NM_001321643.1 , NM_001321643.2
Accession Version	NM_001321643.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	rho-associated protein kinase 2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC099344.4, AB014519.1, AC018463.11, BC111801.1 and AL049383.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.148944.1, SRR1803613.224578.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGGCAG AAGACTATGA TGTTGTAAAA GTTATTGGAA GAGGTGCTTT TGGTGAAGTG 
CAGTTGGTTC GTCACAAGGC ATCGCAGAAG GTTTATGCTA TGAAGCTTCT TAGTAAGTTT 
GAAATGATAA AAAGATCAGA TTCTGCCTTT TTTTGGGAAG AAAGAGATAT TATGGCCTTT 
GCCAATAGCC CCTGGGTGGT TCAGCTTTTT TATGCCTTTC AAGATGATAG GTATCTGTAC 
ATGGTAATGG AGTACATGCC TGGTGGAGAC CTTGTAAACC TTATGAGTAA TTATGATGTG 
CCTGAAAAAT GGGCCAAATT TTACACTGCT GAAGTTGTTC TTGCTCTGGA TGCAATACAC 
TCCATGGGTT TAATACACAG AGATGTGAAG CCTGACAACA TGCTCTTGGA TAAACATGGA 
CATCTAAAAT TAGCAGATTT TGGCACGTGT ATGAAGATGG ATGAAACAGG CATGGTACAT 
TGTGATACAG CAGTTGGAAC ACCGGATTAT ATATCACCTG AGGTTCTGAA ATCACAAGGG 
GGTGATGGTT TCTATGGGCG AGAATGTGAT TGGTGGTCTG TAGGTGTTTT CCTTTATGAG 
ATGCTAGTGG GGGATACTCC ATTTTATGCG GATTCACTTG TAGGAACATA TAGCAAAATT 
ATGGATCATA AGAATTCACT GTGTTTCCCT GAAGATGCAG AAATTTCCAA ACATGCAAAG 
AATCTCATCT GTGCTTTCTT AACAGATAGG GAGGTACGAC TTGGGAGAAA TGGGGTGGAA 
GAAATCAGAC AGCATCCTTT CTTTAAGAAT GATCAGTGGC ATTGGGATAA CATAAGAGAA 
ACGGCAGCTC CTGTAGTACC TGAACTCAGC AGTGACATAG ACAGCAGCAA TTTCGATGAC 
ATTGAAGATG ACAAAGGAGA TGTAGAAACC TTCCCAATTC CTAAAGCTTT TGTTGGAAAT 
CAGCTGCCTT TCATCGGATT TACCTACTAT AGAGAAAATT TATTATTAAG TGACTCTCCA 
TCTTGTAGAG AAACTGATTC CATACAATCA AGGAAAAATG AAGAAAGTCA AGAGATTCAG 
AAAAAACTGT ATACATTAGA AGAACATCTT AGCAATGAGA TGCAAGCCAA AGAGGAACTG 
GAACAGAAGT GCAAATCTGT TAATACTCGC CTAGAAAAAA CAGCAAAGGA GCTAGAAGAG 
GAGATTACCT TACGGAAAAG TGTGGAATCA GCATTAAGAC AGTTAGAAAG AGAAAAGGCG 
CTTCTTCAGC ACAAAAATGC AGAATATCAG AGGAAAGCTG ATCATGAAGC AGACAAAAAA 
CGAAATTTGG AAAATGATGT TAACAGCTTA AAAGATCAAC TTGAAGATTT GAAAAAAAGA 
AATCAAAACT CTCAAATATC CACTGAGAAA GTGAATCAAC TCCAGAGACA ACTGGATGAA 
ACCAATGCTT TACTGCGAAC AGAGTCTGAT ACTGCAGCCC GGTTAAGGAA AACCCAGGCA 
GAAAGTTCAA AACAGATTCA GCAGCTGGAA TCTAACAATA GAGATCTACA AGATAAAAAC 
TGCCTGCTGG AGACTGCCAA GTTAAAACTT GAAAAGGAAT TTATCAATCT TCAGTCAGCT 
CTAGAATCTG AAAGGAGGGA TCGAACCCAT GGATCAGAGA TAATTAATGA TTTACAAGGT 
AGAATATGTG GCCTAGAAGA AGATTTAAAG AACGGCAAAA TCTTACTAGC GAAAGTAGAA 
CTGGAGAAGA GACAACTTCA GGAGAGATTT ACTGATTTGG AAAAGGAAAA AAGCAACATG 
GAAATAGATA TGACATACCA ACTAAAAGTT ATACAGCAGA GCCTAGAACA AGAAGAAGCT 
GAACATAAGG CCACAAAGGC ACGACTAGCA GATAAAAATA AGATCTATGA GTCCATCGAA 
GAAGCCAAAT CAGAAGCCAT GAAAGAAATG GAGAAGAAGC TCTTGGAGGA AAGAACTTTA 
AAACAGAAAG TGGAGAACCT ATTGCTAGAA GCTGAGAAAA GATGTTCTCT ATTAGACTGT 
GACCTCAAAC AGTCACAGCA GAAAATAAAT GAGCTCCTTA AACAGAAAGA TGTGCTAAAT 
GAGGATGTTA GAAACCTGAC ATTAAAAATA GAGCAAGAAA CTCAGAAGCG CTGCCTTACA 
CAAAATGACC TGAAGATGCA AACACAACAG GTTAACACAC TAAAAATGTC AGAAAAGCAG 
TTAAAGCAAG AAAATAACCA TCTCATGGAA ATGAAAATGA ACTTGGAAAA ACAAAATGCT 
GAACTTCGAA AAGAACGTCA GGATGCAGAT GGGCAAATGA AAGAGCTCCA GGATCAGCTC 
GAAGCAGAAC AGTATTTCTC AACCCTTTAT AAAACACAAG TTAGGGAGCT TAAAGAAGAA 
TGTGAAGAAA AGACCAAACT TGGTAAAGAA TTGCAGCAGA AGAAACAGGA ATTACAGGAT 
GAACGGGACT CTTTGGCTGC CCAACTGGAG ATCACCTTGA CCAAAGCAGA TTCTGAGCAA 
CTGGCTCGTT CAATTGCTGA AGAACAATAT TCTGATTTGG AAAAAGAGAA GATCATGAAA 
GAGCTGGAGA TCAAAGAGAT GATGGCTAGA CACAAACAGG AACTTACGGA AAAAGATGCT 
ACAATTGCTT CTCTTGAGGA AACTAATAGG ACACTAACTA GTGATGTTGC CAATCTTGCA 
AATGAGAAAG AAGAATTAAA TAACAAATTG AAAGATGTTC AAGAGCAACT GTCAAGATTG 
AAAGATGAAG AAATAAGCGC AGCAGCTATT AAAGCACAGT TTGAGAAGCA GCTATTAACA 
GAAAGAACAC TCAAAACTCA AGCTGTGAAT AAGTTGGCTG AGATCATGAA TCGAAAAGAA 
CCTGTCAAGC GTGGTAATGA CACAGATGTG CGGAGAAAAG AGAAGGAGAA TAGAAAGCTA 
CATATGGAGC TTAAATCTGA ACGTGAGAAA TTGACCCAGC AGATGATCAA GTATCAGAAA 
GAACTGAATG AAATGCAGGC ACAAATAGCT GAAGAGAGCC AGATTCGAAT TGAACTGCAG 
ATGACATTGG ACAGTAAAGA CAGTGACATT GAGCAGCTGC GGTCACAACT CCAAGCCTTG 
CATATTGGTC TGGATAGTTC CAGTATAGGC AGTGGACCAG GGGATGCTGA GGCAGATGAT 
GGGTTTCCAG AATCAAGATT AGAAGGATGG CTTTCATTGC CTGTACGAAA CAACACTAAG 
AAATTTGGAT GGGTTAAAAA GTATGTGATT GTAAGCAGTA AGAAGATTCT TTTCTATGAC 
AGTGAACAAG ATAAAGAACA ATCCAATCCT TACATGGTTT TAGATATAGA CAAGTTATTT 
CATGTCCGAC CAGTTACACA GACAGATGTG TATAGAGCAG ATGCTAAAGA AATTCCAAGG 
ATATTCCAGA TTCTGTATGC CAATGAAGGA GAAAGTAAGA AGGAACAAGA ATTTCCAGTG 
GAGCCAGTTG GAGAAAAATC TAATTATATT TGCCACAAGG GACATGAGTT TATTCCTACT 
CTTTATCATT TCCCAACCAA CTGTGAGGCT TGTATGAAGC CCCTGTGGCA CATGTTTAAG 
CCTCCTCCTG CTTTGGAGTG CCGCCGTTGC CATATTAAGT GTCATAAAGA TCATATGGAC 
AAAAAGGAGG AGATTATAGC ACCTTGCAAA GTATATTATG ATATTTCAAC GGCAAAGAAT 
CTGTTATTAC TAGCAAATTC TACAGAAGAG CAGCAGAAGT GGGTTAGTCG GTTGGTGAAA 
AAGATACCTA AAAAGCCCCC AGCTCCAGAC CCTTTTGCCC GATCATCTCC TAGAACTTCA 
ATGAAGATAC AGCAAAACCA GTCTATTAGA CGGCCAAGTC GACAGCTTGC CCCAAACAAA 
CCTAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001308572.1
CDS	252..4160
Translation	MKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGFYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDAEISKHAKNLICAFLTDREVRLGRNGVEEIRQHPFFKNDQWHWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYRENLLLSDSPSCRETDSIQSRKNEESQEIQKKLYTLEEHLSNEMQAKEELEQKCKSVNTRLEKTAKELEEEITLRKSVESALRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQNSQISTEKVNQLQRQLDETNALLRTESDTAARLRKTQAESSKQIQQLESNNRDLQDKNCLLETAKLKLEKEFINLQSALESERRDRTHGSEIINDLQGRICGLEEDLKNGKILLAKVELEKRQLQERFTDLEKEKSNMEIDMTYQLKVIQQSLEQEEAEHKATKARLADKNKIYESIEEAKSEAMKEMEKKLLEERTLKQKVENLLLEAEKRCSLLDCDLKQSQQKINELLKQKDVLNEDVRNLTLKIEQETQKRCLTQNDLKMQTQQVNTLKMSEKQLKQENNHLMEMKMNLEKQNAELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEECEEKTKLGKELQQKKQELQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELTEKDATIASLEETNRTLTSDVANLANEKEELNNKLKDVQEQLSRLKDEEISAAAIKAQFEKQLLTERTLKTQAVNKLAEIMNRKEPVKRGNDTDVRRKEKENRKLHMELKSEREKLTQQMIKYQKELNEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQALHIGLDSSSIGSGPGDAEADDGFPESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEQEFPVEPVGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISTAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPS

Target ORF information:

RefSeq Version	NM_001321643.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001321643.1

ORF Insert Sequence:

ATGAAGGCAG AAGACTATGA TGTTGTAAAA GTTATTGGAA GAGGTGCTTT TGGTGAAGTG 
CAGTTGGTTC GTCACAAGGC ATCGCAGAAG GTTTATGCTA TGAAGCTTCT TAGTAAGTTT 
GAAATGATAA AAAGATCAGA TTCTGCCTTT TTTTGGGAAG AAAGAGATAT TATGGCCTTT 
GCCAATAGCC CCTGGGTGGT TCAGCTTTTT TATGCCTTTC AAGATGATAG GTATCTGTAC 
ATGGTAATGG AGTACATGCC TGGTGGAGAC CTTGTAAACC TTATGAGTAA TTATGATGTG 
CCTGAAAAAT GGGCCAAATT TTACACTGCT GAAGTTGTTC TTGCTCTGGA TGCAATACAC 
TCCATGGGTT TAATACACAG AGATGTGAAG CCTGACAACA TGCTCTTGGA TAAACATGGA 
CATCTAAAAT TAGCAGATTT TGGCACGTGT ATGAAGATGG ATGAAACAGG CATGGTACAT 
TGTGATACAG CAGTTGGAAC ACCGGATTAT ATATCACCTG AGGTTCTGAA ATCACAAGGG 
GGTGATGGTT TCTATGGGCG AGAATGTGAT TGGTGGTCTG TAGGTGTTTT CCTTTATGAG 
ATGCTAGTGG GGGATACTCC ATTTTATGCG GATTCACTTG TAGGAACATA TAGCAAAATT 
ATGGATCATA AGAATTCACT GTGTTTCCCT GAAGATGCAG AAATTTCCAA ACATGCAAAG 
AATCTCATCT GTGCTTTCTT AACAGATAGG GAGGTACGAC TTGGGAGAAA TGGGGTGGAA 
GAAATCAGAC AGCATCCTTT CTTTAAGAAT GATCAGTGGC ATTGGGATAA CATAAGAGAA 
ACGGCAGCTC CTGTAGTACC TGAACTCAGC AGTGACATAG ACAGCAGCAA TTTCGATGAC 
ATTGAAGATG ACAAAGGAGA TGTAGAAACC TTCCCAATTC CTAAAGCTTT TGTTGGAAAT 
CAGCTGCCTT TCATCGGATT TACCTACTAT AGAGAAAATT TATTATTAAG TGACTCTCCA 
TCTTGTAGAG AAACTGATTC CATACAATCA AGGAAAAATG AAGAAAGTCA AGAGATTCAG 
AAAAAACTGT ATACATTAGA AGAACATCTT AGCAATGAGA TGCAAGCCAA AGAGGAACTG 
GAACAGAAGT GCAAATCTGT TAATACTCGC CTAGAAAAAA CAGCAAAGGA GCTAGAAGAG 
GAGATTACCT TACGGAAAAG TGTGGAATCA GCATTAAGAC AGTTAGAAAG AGAAAAGGCG 
CTTCTTCAGC ACAAAAATGC AGAATATCAG AGGAAAGCTG ATCATGAAGC AGACAAAAAA 
CGAAATTTGG AAAATGATGT TAACAGCTTA AAAGATCAAC TTGAAGATTT GAAAAAAAGA 
AATCAAAACT CTCAAATATC CACTGAGAAA GTGAATCAAC TCCAGAGACA ACTGGATGAA 
ACCAATGCTT TACTGCGAAC AGAGTCTGAT ACTGCAGCCC GGTTAAGGAA AACCCAGGCA 
GAAAGTTCAA AACAGATTCA GCAGCTGGAA TCTAACAATA GAGATCTACA AGATAAAAAC 
TGCCTGCTGG AGACTGCCAA GTTAAAACTT GAAAAGGAAT TTATCAATCT TCAGTCAGCT 
CTAGAATCTG AAAGGAGGGA TCGAACCCAT GGATCAGAGA TAATTAATGA TTTACAAGGT 
AGAATATGTG GCCTAGAAGA AGATTTAAAG AACGGCAAAA TCTTACTAGC GAAAGTAGAA 
CTGGAGAAGA GACAACTTCA GGAGAGATTT ACTGATTTGG AAAAGGAAAA AAGCAACATG 
GAAATAGATA TGACATACCA ACTAAAAGTT ATACAGCAGA GCCTAGAACA AGAAGAAGCT 
GAACATAAGG CCACAAAGGC ACGACTAGCA GATAAAAATA AGATCTATGA GTCCATCGAA 
GAAGCCAAAT CAGAAGCCAT GAAAGAAATG GAGAAGAAGC TCTTGGAGGA AAGAACTTTA 
AAACAGAAAG TGGAGAACCT ATTGCTAGAA GCTGAGAAAA GATGTTCTCT ATTAGACTGT 
GACCTCAAAC AGTCACAGCA GAAAATAAAT GAGCTCCTTA AACAGAAAGA TGTGCTAAAT 
GAGGATGTTA GAAACCTGAC ATTAAAAATA GAGCAAGAAA CTCAGAAGCG CTGCCTTACA 
CAAAATGACC TGAAGATGCA AACACAACAG GTTAACACAC TAAAAATGTC AGAAAAGCAG 
TTAAAGCAAG AAAATAACCA TCTCATGGAA ATGAAAATGA ACTTGGAAAA ACAAAATGCT 
GAACTTCGAA AAGAACGTCA GGATGCAGAT GGGCAAATGA AAGAGCTCCA GGATCAGCTC 
GAAGCAGAAC AGTATTTCTC AACCCTTTAT AAAACACAAG TTAGGGAGCT TAAAGAAGAA 
TGTGAAGAAA AGACCAAACT TGGTAAAGAA TTGCAGCAGA AGAAACAGGA ATTACAGGAT 
GAACGGGACT CTTTGGCTGC CCAACTGGAG ATCACCTTGA CCAAAGCAGA TTCTGAGCAA 
CTGGCTCGTT CAATTGCTGA AGAACAATAT TCTGATTTGG AAAAAGAGAA GATCATGAAA 
GAGCTGGAGA TCAAAGAGAT GATGGCTAGA CACAAACAGG AACTTACGGA AAAAGATGCT 
ACAATTGCTT CTCTTGAGGA AACTAATAGG ACACTAACTA GTGATGTTGC CAATCTTGCA 
AATGAGAAAG AAGAATTAAA TAACAAATTG AAAGATGTTC AAGAGCAACT GTCAAGATTG 
AAAGATGAAG AAATAAGCGC AGCAGCTATT AAAGCACAGT TTGAGAAGCA GCTATTAACA 
GAAAGAACAC TCAAAACTCA AGCTGTGAAT AAGTTGGCTG AGATCATGAA TCGAAAAGAA 
CCTGTCAAGC GTGGTAATGA CACAGATGTG CGGAGAAAAG AGAAGGAGAA TAGAAAGCTA 
CATATGGAGC TTAAATCTGA ACGTGAGAAA TTGACCCAGC AGATGATCAA GTATCAGAAA 
GAACTGAATG AAATGCAGGC ACAAATAGCT GAAGAGAGCC AGATTCGAAT TGAACTGCAG 
ATGACATTGG ACAGTAAAGA CAGTGACATT GAGCAGCTGC GGTCACAACT CCAAGCCTTG 
CATATTGGTC TGGATAGTTC CAGTATAGGC AGTGGACCAG GGGATGCTGA GGCAGATGAT 
GGGTTTCCAG AATCAAGATT AGAAGGATGG CTTTCATTGC CTGTACGAAA CAACACTAAG 
AAATTTGGAT GGGTTAAAAA GTATGTGATT GTAAGCAGTA AGAAGATTCT TTTCTATGAC 
AGTGAACAAG ATAAAGAACA ATCCAATCCT TACATGGTTT TAGATATAGA CAAGTTATTT 
CATGTCCGAC CAGTTACACA GACAGATGTG TATAGAGCAG ATGCTAAAGA AATTCCAAGG 
ATATTCCAGA TTCTGTATGC CAATGAAGGA GAAAGTAAGA AGGAACAAGA ATTTCCAGTG 
GAGCCAGTTG GAGAAAAATC TAATTATATT TGCCACAAGG GACATGAGTT TATTCCTACT 
CTTTATCATT TCCCAACCAA CTGTGAGGCT TGTATGAAGC CCCTGTGGCA CATGTTTAAG 
CCTCCTCCTG CTTTGGAGTG CCGCCGTTGC CATATTAAGT GTCATAAAGA TCATATGGAC 
AAAAAGGAGG AGATTATAGC ACCTTGCAAA GTATATTATG ATATTTCAAC GGCAAAGAAT 
CTGTTATTAC TAGCAAATTC TACAGAAGAG CAGCAGAAGT GGGTTAGTCG GTTGGTGAAA 
AAGATACCTA AAAAGCCCCC AGCTCCAGAC CCTTTTGCCC GATCATCTCC TAGAACTTCA 
ATGAAGATAC AGCAAAACCA GTCTATTAGA CGGCCAAGTC GACAGCTTGC CCCAAACAAA 
CCTAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROCK2 ( NM_001321643.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001308572.1 Homo sapiens Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu102041
Clone ID Related Accession (Same CDS sequence)	NM_001321643.1 , NM_001321643.2
Accession Version	NM_001321643.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	rho-associated protein kinase 2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC099344.4, AB014519.1, AC018463.11, BC111801.1 and AL049383.1. On May 31, 2019 this sequence version replaced NM_001321643.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.148944.1, SRR1803613.224578.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGGCAG AAGACTATGA TGTTGTAAAA GTTATTGGAA GAGGTGCTTT TGGTGAAGTG 
CAGTTGGTTC GTCACAAGGC ATCGCAGAAG GTTTATGCTA TGAAGCTTCT TAGTAAGTTT 
GAAATGATAA AAAGATCAGA TTCTGCCTTT TTTTGGGAAG AAAGAGATAT TATGGCCTTT 
GCCAATAGCC CCTGGGTGGT TCAGCTTTTT TATGCCTTTC AAGATGATAG GTATCTGTAC 
ATGGTAATGG AGTACATGCC TGGTGGAGAC CTTGTAAACC TTATGAGTAA TTATGATGTG 
CCTGAAAAAT GGGCCAAATT TTACACTGCT GAAGTTGTTC TTGCTCTGGA TGCAATACAC 
TCCATGGGTT TAATACACAG AGATGTGAAG CCTGACAACA TGCTCTTGGA TAAACATGGA 
CATCTAAAAT TAGCAGATTT TGGCACGTGT ATGAAGATGG ATGAAACAGG CATGGTACAT 
TGTGATACAG CAGTTGGAAC ACCGGATTAT ATATCACCTG AGGTTCTGAA ATCACAAGGG 
GGTGATGGTT TCTATGGGCG AGAATGTGAT TGGTGGTCTG TAGGTGTTTT CCTTTATGAG 
ATGCTAGTGG GGGATACTCC ATTTTATGCG GATTCACTTG TAGGAACATA TAGCAAAATT 
ATGGATCATA AGAATTCACT GTGTTTCCCT GAAGATGCAG AAATTTCCAA ACATGCAAAG 
AATCTCATCT GTGCTTTCTT AACAGATAGG GAGGTACGAC TTGGGAGAAA TGGGGTGGAA 
GAAATCAGAC AGCATCCTTT CTTTAAGAAT GATCAGTGGC ATTGGGATAA CATAAGAGAA 
ACGGCAGCTC CTGTAGTACC TGAACTCAGC AGTGACATAG ACAGCAGCAA TTTCGATGAC 
ATTGAAGATG ACAAAGGAGA TGTAGAAACC TTCCCAATTC CTAAAGCTTT TGTTGGAAAT 
CAGCTGCCTT TCATCGGATT TACCTACTAT AGAGAAAATT TATTATTAAG TGACTCTCCA 
TCTTGTAGAG AAACTGATTC CATACAATCA AGGAAAAATG AAGAAAGTCA AGAGATTCAG 
AAAAAACTGT ATACATTAGA AGAACATCTT AGCAATGAGA TGCAAGCCAA AGAGGAACTG 
GAACAGAAGT GCAAATCTGT TAATACTCGC CTAGAAAAAA CAGCAAAGGA GCTAGAAGAG 
GAGATTACCT TACGGAAAAG TGTGGAATCA GCATTAAGAC AGTTAGAAAG AGAAAAGGCG 
CTTCTTCAGC ACAAAAATGC AGAATATCAG AGGAAAGCTG ATCATGAAGC AGACAAAAAA 
CGAAATTTGG AAAATGATGT TAACAGCTTA AAAGATCAAC TTGAAGATTT GAAAAAAAGA 
AATCAAAACT CTCAAATATC CACTGAGAAA GTGAATCAAC TCCAGAGACA ACTGGATGAA 
ACCAATGCTT TACTGCGAAC AGAGTCTGAT ACTGCAGCCC GGTTAAGGAA AACCCAGGCA 
GAAAGTTCAA AACAGATTCA GCAGCTGGAA TCTAACAATA GAGATCTACA AGATAAAAAC 
TGCCTGCTGG AGACTGCCAA GTTAAAACTT GAAAAGGAAT TTATCAATCT TCAGTCAGCT 
CTAGAATCTG AAAGGAGGGA TCGAACCCAT GGATCAGAGA TAATTAATGA TTTACAAGGT 
AGAATATGTG GCCTAGAAGA AGATTTAAAG AACGGCAAAA TCTTACTAGC GAAAGTAGAA 
CTGGAGAAGA GACAACTTCA GGAGAGATTT ACTGATTTGG AAAAGGAAAA AAGCAACATG 
GAAATAGATA TGACATACCA ACTAAAAGTT ATACAGCAGA GCCTAGAACA AGAAGAAGCT 
GAACATAAGG CCACAAAGGC ACGACTAGCA GATAAAAATA AGATCTATGA GTCCATCGAA 
GAAGCCAAAT CAGAAGCCAT GAAAGAAATG GAGAAGAAGC TCTTGGAGGA AAGAACTTTA 
AAACAGAAAG TGGAGAACCT ATTGCTAGAA GCTGAGAAAA GATGTTCTCT ATTAGACTGT 
GACCTCAAAC AGTCACAGCA GAAAATAAAT GAGCTCCTTA AACAGAAAGA TGTGCTAAAT 
GAGGATGTTA GAAACCTGAC ATTAAAAATA GAGCAAGAAA CTCAGAAGCG CTGCCTTACA 
CAAAATGACC TGAAGATGCA AACACAACAG GTTAACACAC TAAAAATGTC AGAAAAGCAG 
TTAAAGCAAG AAAATAACCA TCTCATGGAA ATGAAAATGA ACTTGGAAAA ACAAAATGCT 
GAACTTCGAA AAGAACGTCA GGATGCAGAT GGGCAAATGA AAGAGCTCCA GGATCAGCTC 
GAAGCAGAAC AGTATTTCTC AACCCTTTAT AAAACACAAG TTAGGGAGCT TAAAGAAGAA 
TGTGAAGAAA AGACCAAACT TGGTAAAGAA TTGCAGCAGA AGAAACAGGA ATTACAGGAT 
GAACGGGACT CTTTGGCTGC CCAACTGGAG ATCACCTTGA CCAAAGCAGA TTCTGAGCAA 
CTGGCTCGTT CAATTGCTGA AGAACAATAT TCTGATTTGG AAAAAGAGAA GATCATGAAA 
GAGCTGGAGA TCAAAGAGAT GATGGCTAGA CACAAACAGG AACTTACGGA AAAAGATGCT 
ACAATTGCTT CTCTTGAGGA AACTAATAGG ACACTAACTA GTGATGTTGC CAATCTTGCA 
AATGAGAAAG AAGAATTAAA TAACAAATTG AAAGATGTTC AAGAGCAACT GTCAAGATTG 
AAAGATGAAG AAATAAGCGC AGCAGCTATT AAAGCACAGT TTGAGAAGCA GCTATTAACA 
GAAAGAACAC TCAAAACTCA AGCTGTGAAT AAGTTGGCTG AGATCATGAA TCGAAAAGAA 
CCTGTCAAGC GTGGTAATGA CACAGATGTG CGGAGAAAAG AGAAGGAGAA TAGAAAGCTA 
CATATGGAGC TTAAATCTGA ACGTGAGAAA TTGACCCAGC AGATGATCAA GTATCAGAAA 
GAACTGAATG AAATGCAGGC ACAAATAGCT GAAGAGAGCC AGATTCGAAT TGAACTGCAG 
ATGACATTGG ACAGTAAAGA CAGTGACATT GAGCAGCTGC GGTCACAACT CCAAGCCTTG 
CATATTGGTC TGGATAGTTC CAGTATAGGC AGTGGACCAG GGGATGCTGA GGCAGATGAT 
GGGTTTCCAG AATCAAGATT AGAAGGATGG CTTTCATTGC CTGTACGAAA CAACACTAAG 
AAATTTGGAT GGGTTAAAAA GTATGTGATT GTAAGCAGTA AGAAGATTCT TTTCTATGAC 
AGTGAACAAG ATAAAGAACA ATCCAATCCT TACATGGTTT TAGATATAGA CAAGTTATTT 
CATGTCCGAC CAGTTACACA GACAGATGTG TATAGAGCAG ATGCTAAAGA AATTCCAAGG 
ATATTCCAGA TTCTGTATGC CAATGAAGGA GAAAGTAAGA AGGAACAAGA ATTTCCAGTG 
GAGCCAGTTG GAGAAAAATC TAATTATATT TGCCACAAGG GACATGAGTT TATTCCTACT 
CTTTATCATT TCCCAACCAA CTGTGAGGCT TGTATGAAGC CCCTGTGGCA CATGTTTAAG 
CCTCCTCCTG CTTTGGAGTG CCGCCGTTGC CATATTAAGT GTCATAAAGA TCATATGGAC 
AAAAAGGAGG AGATTATAGC ACCTTGCAAA GTATATTATG ATATTTCAAC GGCAAAGAAT 
CTGTTATTAC TAGCAAATTC TACAGAAGAG CAGCAGAAGT GGGTTAGTCG GTTGGTGAAA 
AAGATACCTA AAAAGCCCCC AGCTCCAGAC CCTTTTGCCC GATCATCTCC TAGAACTTCA 
ATGAAGATAC AGCAAAACCA GTCTATTAGA CGGCCAAGTC GACAGCTTGC CCCAAACAAA 
CCTAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001308572.1
CDS	282..4190
Translation	MKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGFYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDAEISKHAKNLICAFLTDREVRLGRNGVEEIRQHPFFKNDQWHWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYRENLLLSDSPSCRETDSIQSRKNEESQEIQKKLYTLEEHLSNEMQAKEELEQKCKSVNTRLEKTAKELEEEITLRKSVESALRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQNSQISTEKVNQLQRQLDETNALLRTESDTAARLRKTQAESSKQIQQLESNNRDLQDKNCLLETAKLKLEKEFINLQSALESERRDRTHGSEIINDLQGRICGLEEDLKNGKILLAKVELEKRQLQERFTDLEKEKSNMEIDMTYQLKVIQQSLEQEEAEHKATKARLADKNKIYESIEEAKSEAMKEMEKKLLEERTLKQKVENLLLEAEKRCSLLDCDLKQSQQKINELLKQKDVLNEDVRNLTLKIEQETQKRCLTQNDLKMQTQQVNTLKMSEKQLKQENNHLMEMKMNLEKQNAELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEECEEKTKLGKELQQKKQELQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELTEKDATIASLEETNRTLTSDVANLANEKEELNNKLKDVQEQLSRLKDEEISAAAIKAQFEKQLLTERTLKTQAVNKLAEIMNRKEPVKRGNDTDVRRKEKENRKLHMELKSEREKLTQQMIKYQKELNEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQALHIGLDSSSIGSGPGDAEADDGFPESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEQEFPVEPVGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISTAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPS

Target ORF information:

RefSeq Version	NM_001321643.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001321643.2

ORF Insert Sequence:

ATGAAGGCAG AAGACTATGA TGTTGTAAAA GTTATTGGAA GAGGTGCTTT TGGTGAAGTG 
CAGTTGGTTC GTCACAAGGC ATCGCAGAAG GTTTATGCTA TGAAGCTTCT TAGTAAGTTT 
GAAATGATAA AAAGATCAGA TTCTGCCTTT TTTTGGGAAG AAAGAGATAT TATGGCCTTT 
GCCAATAGCC CCTGGGTGGT TCAGCTTTTT TATGCCTTTC AAGATGATAG GTATCTGTAC 
ATGGTAATGG AGTACATGCC TGGTGGAGAC CTTGTAAACC TTATGAGTAA TTATGATGTG 
CCTGAAAAAT GGGCCAAATT TTACACTGCT GAAGTTGTTC TTGCTCTGGA TGCAATACAC 
TCCATGGGTT TAATACACAG AGATGTGAAG CCTGACAACA TGCTCTTGGA TAAACATGGA 
CATCTAAAAT TAGCAGATTT TGGCACGTGT ATGAAGATGG ATGAAACAGG CATGGTACAT 
TGTGATACAG CAGTTGGAAC ACCGGATTAT ATATCACCTG AGGTTCTGAA ATCACAAGGG 
GGTGATGGTT TCTATGGGCG AGAATGTGAT TGGTGGTCTG TAGGTGTTTT CCTTTATGAG 
ATGCTAGTGG GGGATACTCC ATTTTATGCG GATTCACTTG TAGGAACATA TAGCAAAATT 
ATGGATCATA AGAATTCACT GTGTTTCCCT GAAGATGCAG AAATTTCCAA ACATGCAAAG 
AATCTCATCT GTGCTTTCTT AACAGATAGG GAGGTACGAC TTGGGAGAAA TGGGGTGGAA 
GAAATCAGAC AGCATCCTTT CTTTAAGAAT GATCAGTGGC ATTGGGATAA CATAAGAGAA 
ACGGCAGCTC CTGTAGTACC TGAACTCAGC AGTGACATAG ACAGCAGCAA TTTCGATGAC 
ATTGAAGATG ACAAAGGAGA TGTAGAAACC TTCCCAATTC CTAAAGCTTT TGTTGGAAAT 
CAGCTGCCTT TCATCGGATT TACCTACTAT AGAGAAAATT TATTATTAAG TGACTCTCCA 
TCTTGTAGAG AAACTGATTC CATACAATCA AGGAAAAATG AAGAAAGTCA AGAGATTCAG 
AAAAAACTGT ATACATTAGA AGAACATCTT AGCAATGAGA TGCAAGCCAA AGAGGAACTG 
GAACAGAAGT GCAAATCTGT TAATACTCGC CTAGAAAAAA CAGCAAAGGA GCTAGAAGAG 
GAGATTACCT TACGGAAAAG TGTGGAATCA GCATTAAGAC AGTTAGAAAG AGAAAAGGCG 
CTTCTTCAGC ACAAAAATGC AGAATATCAG AGGAAAGCTG ATCATGAAGC AGACAAAAAA 
CGAAATTTGG AAAATGATGT TAACAGCTTA AAAGATCAAC TTGAAGATTT GAAAAAAAGA 
AATCAAAACT CTCAAATATC CACTGAGAAA GTGAATCAAC TCCAGAGACA ACTGGATGAA 
ACCAATGCTT TACTGCGAAC AGAGTCTGAT ACTGCAGCCC GGTTAAGGAA AACCCAGGCA 
GAAAGTTCAA AACAGATTCA GCAGCTGGAA TCTAACAATA GAGATCTACA AGATAAAAAC 
TGCCTGCTGG AGACTGCCAA GTTAAAACTT GAAAAGGAAT TTATCAATCT TCAGTCAGCT 
CTAGAATCTG AAAGGAGGGA TCGAACCCAT GGATCAGAGA TAATTAATGA TTTACAAGGT 
AGAATATGTG GCCTAGAAGA AGATTTAAAG AACGGCAAAA TCTTACTAGC GAAAGTAGAA 
CTGGAGAAGA GACAACTTCA GGAGAGATTT ACTGATTTGG AAAAGGAAAA AAGCAACATG 
GAAATAGATA TGACATACCA ACTAAAAGTT ATACAGCAGA GCCTAGAACA AGAAGAAGCT 
GAACATAAGG CCACAAAGGC ACGACTAGCA GATAAAAATA AGATCTATGA GTCCATCGAA 
GAAGCCAAAT CAGAAGCCAT GAAAGAAATG GAGAAGAAGC TCTTGGAGGA AAGAACTTTA 
AAACAGAAAG TGGAGAACCT ATTGCTAGAA GCTGAGAAAA GATGTTCTCT ATTAGACTGT 
GACCTCAAAC AGTCACAGCA GAAAATAAAT GAGCTCCTTA AACAGAAAGA TGTGCTAAAT 
GAGGATGTTA GAAACCTGAC ATTAAAAATA GAGCAAGAAA CTCAGAAGCG CTGCCTTACA 
CAAAATGACC TGAAGATGCA AACACAACAG GTTAACACAC TAAAAATGTC AGAAAAGCAG 
TTAAAGCAAG AAAATAACCA TCTCATGGAA ATGAAAATGA ACTTGGAAAA ACAAAATGCT 
GAACTTCGAA AAGAACGTCA GGATGCAGAT GGGCAAATGA AAGAGCTCCA GGATCAGCTC 
GAAGCAGAAC AGTATTTCTC AACCCTTTAT AAAACACAAG TTAGGGAGCT TAAAGAAGAA 
TGTGAAGAAA AGACCAAACT TGGTAAAGAA TTGCAGCAGA AGAAACAGGA ATTACAGGAT 
GAACGGGACT CTTTGGCTGC CCAACTGGAG ATCACCTTGA CCAAAGCAGA TTCTGAGCAA 
CTGGCTCGTT CAATTGCTGA AGAACAATAT TCTGATTTGG AAAAAGAGAA GATCATGAAA 
GAGCTGGAGA TCAAAGAGAT GATGGCTAGA CACAAACAGG AACTTACGGA AAAAGATGCT 
ACAATTGCTT CTCTTGAGGA AACTAATAGG ACACTAACTA GTGATGTTGC CAATCTTGCA 
AATGAGAAAG AAGAATTAAA TAACAAATTG AAAGATGTTC AAGAGCAACT GTCAAGATTG 
AAAGATGAAG AAATAAGCGC AGCAGCTATT AAAGCACAGT TTGAGAAGCA GCTATTAACA 
GAAAGAACAC TCAAAACTCA AGCTGTGAAT AAGTTGGCTG AGATCATGAA TCGAAAAGAA 
CCTGTCAAGC GTGGTAATGA CACAGATGTG CGGAGAAAAG AGAAGGAGAA TAGAAAGCTA 
CATATGGAGC TTAAATCTGA ACGTGAGAAA TTGACCCAGC AGATGATCAA GTATCAGAAA 
GAACTGAATG AAATGCAGGC ACAAATAGCT GAAGAGAGCC AGATTCGAAT TGAACTGCAG 
ATGACATTGG ACAGTAAAGA CAGTGACATT GAGCAGCTGC GGTCACAACT CCAAGCCTTG 
CATATTGGTC TGGATAGTTC CAGTATAGGC AGTGGACCAG GGGATGCTGA GGCAGATGAT 
GGGTTTCCAG AATCAAGATT AGAAGGATGG CTTTCATTGC CTGTACGAAA CAACACTAAG 
AAATTTGGAT GGGTTAAAAA GTATGTGATT GTAAGCAGTA AGAAGATTCT TTTCTATGAC 
AGTGAACAAG ATAAAGAACA ATCCAATCCT TACATGGTTT TAGATATAGA CAAGTTATTT 
CATGTCCGAC CAGTTACACA GACAGATGTG TATAGAGCAG ATGCTAAAGA AATTCCAAGG 
ATATTCCAGA TTCTGTATGC CAATGAAGGA GAAAGTAAGA AGGAACAAGA ATTTCCAGTG 
GAGCCAGTTG GAGAAAAATC TAATTATATT TGCCACAAGG GACATGAGTT TATTCCTACT 
CTTTATCATT TCCCAACCAA CTGTGAGGCT TGTATGAAGC CCCTGTGGCA CATGTTTAAG 
CCTCCTCCTG CTTTGGAGTG CCGCCGTTGC CATATTAAGT GTCATAAAGA TCATATGGAC 
AAAAAGGAGG AGATTATAGC ACCTTGCAAA GTATATTATG ATATTTCAAC GGCAAAGAAT 
CTGTTATTAC TAGCAAATTC TACAGAAGAG CAGCAGAAGT GGGTTAGTCG GTTGGTGAAA 
AAGATACCTA AAAAGCCCCC AGCTCCAGAC CCTTTTGCCC GATCATCTCC TAGAACTTCA 
ATGAAGATAC AGCAAAACCA GTCTATTAGA CGGCCAAGTC GACAGCTTGC CCCAAACAAA 
CCTAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROCK2 ( XM_017005379.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860868.1 Homo sapiens Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X4, mRNA.

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CloneID	OHu62859
Clone ID Related Accession (Same CDS sequence)	XM_017005379.1 , XM_011510417.2 , XM_017005379.2
Accession Version	XM_017005379.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rho-associated protein kinase 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCAG AAGACTATGA TGTTGTAAAA GTTATTGGAA GAGGTGCTTT TGGTGAAGTG 
CAGTTGGTTC GTCACAAGGC ATCGCAGAAG GTTTATGCTA TGAAGCTTCT TAGTAAGTTT 
GAAATGATAA AAAGATCAGA TTCTGCCTTT TTTTGGGAAG AAAGAGATAT TATGGCCTTT 
GCCAATAGCC CCTGGGTGGT TCAGCTTTTT TATGCCTTTC AAGATGATAG GTATCTGTAC 
ATGGTAATGG AGTACATGCC TGGTGGAGAC CTTGTAAACC TTATGAGTAA TTATGATGTG 
CCTGAAAAAT GGGCCAAATT TTACACTGCT GAAGTTGTTC TTGCTCTGGA TGCAATACAC 
TCCATGGGTT TAATACACAG AGATGTGAAG CCTGACAACA TGCTCTTGGA TAAACATGGA 
CATCTAAAAT TAGCAGATTT TGGCACGTGT ATGAAGATGG ATGAAACAGG CATGGTACAT 
TGTGATACAG CAGTTGGAAC ACCGGATTAT ATATCACCTG AGGTTCTGAA ATCACAAGGG 
GGTGATGGTT TCTATGGGCG AGAATGTGAT TGGTGGTCTG TAGGTGTTTT CCTTTATGAG 
ATGCTAGTGG GGGATACTCC ATTTTATGCG GATTCACTTG TAGGAACATA TAGCAAAATT 
ATGGATCATA AGAATTCACT GTGTTTCCCT GAAGATGCAG AAATTTCCAA ACATGCAAAG 
AATCTCATCT GTGCTTTCTT AACAGATAGG GAGGTACGAC TTGGGAGAAA TGGGGTGGAA 
GAAATCAGAC AGCATCCTTT CTTTAAGAAT GATCAGTGGC ATTGGGATAA CATAAGAGAA 
ACGGCAGCTC CTGTAGTACC TGAACTCAGC AGTGACATAG ACAGCAGCAA TTTCGATGAC 
ATTGAAGATG ACAAAGGAGA TGTAGAAACC TTCCCAATTC CTAAAGCTTT TGTTGGAAAT 
CAGCTGCCTT TCATCGGATT TACCTACTAT AGAGAAAATT TATTATTAAG TGACTCTCCA 
TCTTGTAGAG AAACTGATTC CATACAATCA AGGAAAAATG AAGAAAGTCA AGAGATTCAG 
AAAAAACTGT ATACATTAGA AGAACATCTT AGCAATGAGA TGCAAGCCAA AGAGGAACTG 
GAACAGAAGT GCAAATCTGT TAATACTCGC CTAGAAAAAA CAGCAAAGGA GCTAGAAGAG 
GAGATTACCT TACGGAAAAG TGTGGAATCA GCATTAAGAC AGTTAGAAAG AGAAAAGGCG 
CTTCTTCAGC ACAAAAATGC AGAATATCAG AGGAAAGCTG ATCATGAAGC AGACAAAAAA 
CGAAATTTGG AAAATGATGT TAACAGCTTA AAAGATCAAC TTGAAGATTT GAAAAAAAGA 
AATCAAAACT CTCAAATATC CACTGAGAAA GTGAATCAAC TCCAGAGACA ACTGGATGAA 
ACCAATGCTT TACTGCGAAC AGAGTCTGAT ACTGCAGCCC GGTTAAGGAA AACCCAGGCA 
GAAAGTTCAA AACAGATTCA GCAGCTGGAA TCTAACAATA GAGATCTACA AGATAAAAAC 
TGCCTGCTGG AGACTGCCAA GTTAAAACTT GAAAAGGAAT TTATCAATCT TCAGTCAGCT 
CTAGAATCTG AAAGGAGGGA TCGAACCCAT GGATCAGAGA TAATTAATGA TTTACAAGGT 
AGAATATGTG GCCTAGAAGA AGATTTAAAG AACGGCAAAA TCTTACTAGC GAAAGTAGAA 
CTGGAGAAGA GACAACTTCA GGAGAGATTT ACTGATTTGG AAAAGGAAAA AAGCAACATG 
GAAATAGATA TGACATACCA ACTAAAAGTT ATACAGCAGA GCCTAGAACA AGAAGAAGCT 
GAACATAAGG CCACAAAGGC ACGACTAGCA GATAAAAATA AGATCTATGA GTCCATCGAA 
GAAGCCAAAT CAGAAGCCAT GAAAGAAATG GAGAAGAAGC TCTTGGAGGA AAGAACTTTA 
AAACAGAAAG TGGAGAACCT ATTGCTAGAA GCTGAGAAAA GATGTTCTCT ATTAGACTGT 
GACCTCAAAC AGTCACAGCA GAAAATAAAT GAGCTCCTTA AACAGAAAGA TGTGCTAAAT 
GAGGATGTTA GAAACCTGAC ATTAAAAATA GAGCAAGAAA CTCAGAAGCG CTGCCTTACA 
CAAAATGACC TGAAGATGCA AACACAACAG GTTAACACAC TAAAAATGTC AGAAAAGCAG 
TTAAAGCAAG AAAATAACCA TCTCATGGAA ATGAAAATGA ACTTGGAAAA ACAAAATGCT 
GAACTTCGAA AAGAACGTCA GGATGCAGAT GGGCAAATGA AAGAGCTCCA GGATCAGCTC 
GAAGCAGAAC AGTATTTCTC AACCCTTTAT AAAACACAAG TTAGGGAGCT TAAAGAAGAA 
TGTGAAGAAA AGACCAAACT TGGTAAAGAA TTGCAGCAGA AGAAACAGGA ATTACAGGAT 
GAACGGGACT CTTTGGCTGC CCAACTGGAG ATCACCTTGA CCAAAGCAGA TTCTGAGCAA 
CTGGCTCGTT CAATTGCTGA AGAACAATAT TCTGATTTGG AAAAAGAGAA GATCATGAAA 
GAGCTGGAGA TCAAAGAGAT GATGGCTAGA CACAAACAGG AACTTACGGA AAAAGATGCT 
ACAATTGCTT CTCTTGAGGA AACTAATAGG ACACTAACTA GTGATGTTGC CAATCTTGCA 
AATGAGAAAG AAGAATTAAA TAACAAATTG AAAGATGTTC AAGAGCAACT GTCAAGATTG 
AAAGATGAAG AAATAAGCGC AGCAGCTATT AAAGCACAGT TTGAGAAGCA GCTATTAACA 
GAAAGAACAC TCAAAACTCA AGCTGTGAAT AAGTTGGCTG AGATCATGAA TCGAAAAGAA 
CCTGTCAAGC GTGGTAATGA CACAGATGTG CGGAGAAAAG AGAAGGAGAA TAGAAAGCTA 
CATATGGAGC TTAAATCTGA ACGTGAGAAA TTGACCCAGC AGATGATCAA GTATCAGAAA 
GAACTGAATG AAATGCAGGC ACAAATAGCT GAAGAGAGCC AGATTCGAAT TGAACTGCAG 
ATGACATTGG ACAGTAAAGA CAGTGACATT GAGCAGCTGC GGTCACAACT CCAAGCCTTG 
CATATTGGTC TGGATAGTTC CAGTATAGGC AGTGGACCAG GGGATGCTGA GGCAGATGAT 
GGGTTTCCAG AATCAAGATT AGAAGGATGG CTTTCATTGC CTGTACGAAA CAACACTAAG 
AAATTTGGAT GGGTTAAAAA GTATGTGATT GTAAGCAGTA AGAAGATTCT TTTCTATGAC 
AGTGAACAAG ATAAAGAACA ATCCAATCCT TACATGGTTT TAGATATAGA CAAGTTATTT 
CATGTCCGAC CAGTTACACA GACAGATGTG TATAGAGCAG ATGCTAAAGA AATTCCAAGG 
ATATTCCAGA TTCTGTATGC CAATGAAGGA GAAAGTAAGA AGGAACAAGA ATTTCCAGTG 
GAGCCAGTTG GAGAAAAATC TAATTATATT TGCCACAAGG GACATGAGTT TATTCCTACT 
CTTTATCATT TCCCAACCAA CTGTGAGGCT TGTATGAAGC CCCTGTGGCA CATGTTTAAG 
CCTCCTCCTG CTTTGGAGTG CCGCCGTTGC CATATTAAGT GTCATAAAGA TCATATGGAC 
AAAAAGGAGG AGATTATAGC ACCTTGCAAA GTATATTATG ATATTTCAAC GGCAAAGAAT 
CTGTTATTAC TAGCAAATTC TACAGAAGAG CAGCAGAAGT GGGTTAGTCG GTTGGTGAAA 
AAGATACCTA AAAAGCCCCC AGCTCCAGAC CCTTTTGCCC GATCATCTCC TAGAACTTCA 
ATGAAGATAC AGCAAAACCA GTCTATTAGA CGGCCAAGTC GACAGCTTGC CCCAAACAAA 
CCTAGATTGC TTGATTTGGC ATTTAAAGAC TGGGATTGGT CATTTGATGA TGTTGATGGT 
GGTGGTGATG ATGATGACGA TGTTTTTGAT TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860868.1
CDS	137..4132
Translation	MKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGFYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDAEISKHAKNLICAFLTDREVRLGRNGVEEIRQHPFFKNDQWHWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYRENLLLSDSPSCRETDSIQSRKNEESQEIQKKLYTLEEHLSNEMQAKEELEQKCKSVNTRLEKTAKELEEEITLRKSVESALRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQNSQISTEKVNQLQRQLDETNALLRTESDTAARLRKTQAESSKQIQQLESNNRDLQDKNCLLETAKLKLEKEFINLQSALESERRDRTHGSEIINDLQGRICGLEEDLKNGKILLAKVELEKRQLQERFTDLEKEKSNMEIDMTYQLKVIQQSLEQEEAEHKATKARLADKNKIYESIEEAKSEAMKEMEKKLLEERTLKQKVENLLLEAEKRCSLLDCDLKQSQQKINELLKQKDVLNEDVRNLTLKIEQETQKRCLTQNDLKMQTQQVNTLKMSEKQLKQENNHLMEMKMNLEKQNAELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEECEEKTKLGKELQQKKQELQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELTEKDATIASLEETNRTLTSDVANLANEKEELNNKLKDVQEQLSRLKDEEISAAAIKAQFEKQLLTERTLKTQAVNKLAEIMNRKEPVKRGNDTDVRRKEKENRKLHMELKSEREKLTQQMIKYQKELNEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQALHIGLDSSSIGSGPGDAEADDGFPESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEQEFPVEPVGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISTAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPRLLDLAFKDWDWSFDDVDGGGDDDDDVFDF

Target ORF information:

RefSeq Version	XM_017005379.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005379.1

ORF Insert Sequence:

ATGAAGGCAG AAGACTATGA TGTTGTAAAA GTTATTGGAA GAGGTGCTTT TGGTGAAGTG 
CAGTTGGTTC GTCACAAGGC ATCGCAGAAG GTTTATGCTA TGAAGCTTCT TAGTAAGTTT 
GAAATGATAA AAAGATCAGA TTCTGCCTTT TTTTGGGAAG AAAGAGATAT TATGGCCTTT 
GCCAATAGCC CCTGGGTGGT TCAGCTTTTT TATGCCTTTC AAGATGATAG GTATCTGTAC 
ATGGTAATGG AGTACATGCC TGGTGGAGAC CTTGTAAACC TTATGAGTAA TTATGATGTG 
CCTGAAAAAT GGGCCAAATT TTACACTGCT GAAGTTGTTC TTGCTCTGGA TGCAATACAC 
TCCATGGGTT TAATACACAG AGATGTGAAG CCTGACAACA TGCTCTTGGA TAAACATGGA 
CATCTAAAAT TAGCAGATTT TGGCACGTGT ATGAAGATGG ATGAAACAGG CATGGTACAT 
TGTGATACAG CAGTTGGAAC ACCGGATTAT ATATCACCTG AGGTTCTGAA ATCACAAGGG 
GGTGATGGTT TCTATGGGCG AGAATGTGAT TGGTGGTCTG TAGGTGTTTT CCTTTATGAG 
ATGCTAGTGG GGGATACTCC ATTTTATGCG GATTCACTTG TAGGAACATA TAGCAAAATT 
ATGGATCATA AGAATTCACT GTGTTTCCCT GAAGATGCAG AAATTTCCAA ACATGCAAAG 
AATCTCATCT GTGCTTTCTT AACAGATAGG GAGGTACGAC TTGGGAGAAA TGGGGTGGAA 
GAAATCAGAC AGCATCCTTT CTTTAAGAAT GATCAGTGGC ATTGGGATAA CATAAGAGAA 
ACGGCAGCTC CTGTAGTACC TGAACTCAGC AGTGACATAG ACAGCAGCAA TTTCGATGAC 
ATTGAAGATG ACAAAGGAGA TGTAGAAACC TTCCCAATTC CTAAAGCTTT TGTTGGAAAT 
CAGCTGCCTT TCATCGGATT TACCTACTAT AGAGAAAATT TATTATTAAG TGACTCTCCA 
TCTTGTAGAG AAACTGATTC CATACAATCA AGGAAAAATG AAGAAAGTCA AGAGATTCAG 
AAAAAACTGT ATACATTAGA AGAACATCTT AGCAATGAGA TGCAAGCCAA AGAGGAACTG 
GAACAGAAGT GCAAATCTGT TAATACTCGC CTAGAAAAAA CAGCAAAGGA GCTAGAAGAG 
GAGATTACCT TACGGAAAAG TGTGGAATCA GCATTAAGAC AGTTAGAAAG AGAAAAGGCG 
CTTCTTCAGC ACAAAAATGC AGAATATCAG AGGAAAGCTG ATCATGAAGC AGACAAAAAA 
CGAAATTTGG AAAATGATGT TAACAGCTTA AAAGATCAAC TTGAAGATTT GAAAAAAAGA 
AATCAAAACT CTCAAATATC CACTGAGAAA GTGAATCAAC TCCAGAGACA ACTGGATGAA 
ACCAATGCTT TACTGCGAAC AGAGTCTGAT ACTGCAGCCC GGTTAAGGAA AACCCAGGCA 
GAAAGTTCAA AACAGATTCA GCAGCTGGAA TCTAACAATA GAGATCTACA AGATAAAAAC 
TGCCTGCTGG AGACTGCCAA GTTAAAACTT GAAAAGGAAT TTATCAATCT TCAGTCAGCT 
CTAGAATCTG AAAGGAGGGA TCGAACCCAT GGATCAGAGA TAATTAATGA TTTACAAGGT 
AGAATATGTG GCCTAGAAGA AGATTTAAAG AACGGCAAAA TCTTACTAGC GAAAGTAGAA 
CTGGAGAAGA GACAACTTCA GGAGAGATTT ACTGATTTGG AAAAGGAAAA AAGCAACATG 
GAAATAGATA TGACATACCA ACTAAAAGTT ATACAGCAGA GCCTAGAACA AGAAGAAGCT 
GAACATAAGG CCACAAAGGC ACGACTAGCA GATAAAAATA AGATCTATGA GTCCATCGAA 
GAAGCCAAAT CAGAAGCCAT GAAAGAAATG GAGAAGAAGC TCTTGGAGGA AAGAACTTTA 
AAACAGAAAG TGGAGAACCT ATTGCTAGAA GCTGAGAAAA GATGTTCTCT ATTAGACTGT 
GACCTCAAAC AGTCACAGCA GAAAATAAAT GAGCTCCTTA AACAGAAAGA TGTGCTAAAT 
GAGGATGTTA GAAACCTGAC ATTAAAAATA GAGCAAGAAA CTCAGAAGCG CTGCCTTACA 
CAAAATGACC TGAAGATGCA AACACAACAG GTTAACACAC TAAAAATGTC AGAAAAGCAG 
TTAAAGCAAG AAAATAACCA TCTCATGGAA ATGAAAATGA ACTTGGAAAA ACAAAATGCT 
GAACTTCGAA AAGAACGTCA GGATGCAGAT GGGCAAATGA AAGAGCTCCA GGATCAGCTC 
GAAGCAGAAC AGTATTTCTC AACCCTTTAT AAAACACAAG TTAGGGAGCT TAAAGAAGAA 
TGTGAAGAAA AGACCAAACT TGGTAAAGAA TTGCAGCAGA AGAAACAGGA ATTACAGGAT 
GAACGGGACT CTTTGGCTGC CCAACTGGAG ATCACCTTGA CCAAAGCAGA TTCTGAGCAA 
CTGGCTCGTT CAATTGCTGA AGAACAATAT TCTGATTTGG AAAAAGAGAA GATCATGAAA 
GAGCTGGAGA TCAAAGAGAT GATGGCTAGA CACAAACAGG AACTTACGGA AAAAGATGCT 
ACAATTGCTT CTCTTGAGGA AACTAATAGG ACACTAACTA GTGATGTTGC CAATCTTGCA 
AATGAGAAAG AAGAATTAAA TAACAAATTG AAAGATGTTC AAGAGCAACT GTCAAGATTG 
AAAGATGAAG AAATAAGCGC AGCAGCTATT AAAGCACAGT TTGAGAAGCA GCTATTAACA 
GAAAGAACAC TCAAAACTCA AGCTGTGAAT AAGTTGGCTG AGATCATGAA TCGAAAAGAA 
CCTGTCAAGC GTGGTAATGA CACAGATGTG CGGAGAAAAG AGAAGGAGAA TAGAAAGCTA 
CATATGGAGC TTAAATCTGA ACGTGAGAAA TTGACCCAGC AGATGATCAA GTATCAGAAA 
GAACTGAATG AAATGCAGGC ACAAATAGCT GAAGAGAGCC AGATTCGAAT TGAACTGCAG 
ATGACATTGG ACAGTAAAGA CAGTGACATT GAGCAGCTGC GGTCACAACT CCAAGCCTTG 
CATATTGGTC TGGATAGTTC CAGTATAGGC AGTGGACCAG GGGATGCTGA GGCAGATGAT 
GGGTTTCCAG AATCAAGATT AGAAGGATGG CTTTCATTGC CTGTACGAAA CAACACTAAG 
AAATTTGGAT GGGTTAAAAA GTATGTGATT GTAAGCAGTA AGAAGATTCT TTTCTATGAC 
AGTGAACAAG ATAAAGAACA ATCCAATCCT TACATGGTTT TAGATATAGA CAAGTTATTT 
CATGTCCGAC CAGTTACACA GACAGATGTG TATAGAGCAG ATGCTAAAGA AATTCCAAGG 
ATATTCCAGA TTCTGTATGC CAATGAAGGA GAAAGTAAGA AGGAACAAGA ATTTCCAGTG 
GAGCCAGTTG GAGAAAAATC TAATTATATT TGCCACAAGG GACATGAGTT TATTCCTACT 
CTTTATCATT TCCCAACCAA CTGTGAGGCT TGTATGAAGC CCCTGTGGCA CATGTTTAAG 
CCTCCTCCTG CTTTGGAGTG CCGCCGTTGC CATATTAAGT GTCATAAAGA TCATATGGAC 
AAAAAGGAGG AGATTATAGC ACCTTGCAAA GTATATTATG ATATTTCAAC GGCAAAGAAT 
CTGTTATTAC TAGCAAATTC TACAGAAGAG CAGCAGAAGT GGGTTAGTCG GTTGGTGAAA 
AAGATACCTA AAAAGCCCCC AGCTCCAGAC CCTTTTGCCC GATCATCTCC TAGAACTTCA 
ATGAAGATAC AGCAAAACCA GTCTATTAGA CGGCCAAGTC GACAGCTTGC CCCAAACAAA 
CCTAGATTGC TTGATTTGGC ATTTAAAGAC TGGGATTGGT CATTTGATGA TGTTGATGGT 
GGTGGTGATG ATGATGACGA TGTTTTTGAT TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROCK2 ( XM_011510417.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508719.1 Homo sapiens Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X4, mRNA.

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CloneID	OHu62859
Clone ID Related Accession (Same CDS sequence)	XM_017005379.1 , XM_011510417.2 , XM_017005379.2
Accession Version	XM_011510417.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rho-associated protein kinase 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510417.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCAG AAGACTATGA TGTTGTAAAA GTTATTGGAA GAGGTGCTTT TGGTGAAGTG 
CAGTTGGTTC GTCACAAGGC ATCGCAGAAG GTTTATGCTA TGAAGCTTCT TAGTAAGTTT 
GAAATGATAA AAAGATCAGA TTCTGCCTTT TTTTGGGAAG AAAGAGATAT TATGGCCTTT 
GCCAATAGCC CCTGGGTGGT TCAGCTTTTT TATGCCTTTC AAGATGATAG GTATCTGTAC 
ATGGTAATGG AGTACATGCC TGGTGGAGAC CTTGTAAACC TTATGAGTAA TTATGATGTG 
CCTGAAAAAT GGGCCAAATT TTACACTGCT GAAGTTGTTC TTGCTCTGGA TGCAATACAC 
TCCATGGGTT TAATACACAG AGATGTGAAG CCTGACAACA TGCTCTTGGA TAAACATGGA 
CATCTAAAAT TAGCAGATTT TGGCACGTGT ATGAAGATGG ATGAAACAGG CATGGTACAT 
TGTGATACAG CAGTTGGAAC ACCGGATTAT ATATCACCTG AGGTTCTGAA ATCACAAGGG 
GGTGATGGTT TCTATGGGCG AGAATGTGAT TGGTGGTCTG TAGGTGTTTT CCTTTATGAG 
ATGCTAGTGG GGGATACTCC ATTTTATGCG GATTCACTTG TAGGAACATA TAGCAAAATT 
ATGGATCATA AGAATTCACT GTGTTTCCCT GAAGATGCAG AAATTTCCAA ACATGCAAAG 
AATCTCATCT GTGCTTTCTT AACAGATAGG GAGGTACGAC TTGGGAGAAA TGGGGTGGAA 
GAAATCAGAC AGCATCCTTT CTTTAAGAAT GATCAGTGGC ATTGGGATAA CATAAGAGAA 
ACGGCAGCTC CTGTAGTACC TGAACTCAGC AGTGACATAG ACAGCAGCAA TTTCGATGAC 
ATTGAAGATG ACAAAGGAGA TGTAGAAACC TTCCCAATTC CTAAAGCTTT TGTTGGAAAT 
CAGCTGCCTT TCATCGGATT TACCTACTAT AGAGAAAATT TATTATTAAG TGACTCTCCA 
TCTTGTAGAG AAACTGATTC CATACAATCA AGGAAAAATG AAGAAAGTCA AGAGATTCAG 
AAAAAACTGT ATACATTAGA AGAACATCTT AGCAATGAGA TGCAAGCCAA AGAGGAACTG 
GAACAGAAGT GCAAATCTGT TAATACTCGC CTAGAAAAAA CAGCAAAGGA GCTAGAAGAG 
GAGATTACCT TACGGAAAAG TGTGGAATCA GCATTAAGAC AGTTAGAAAG AGAAAAGGCG 
CTTCTTCAGC ACAAAAATGC AGAATATCAG AGGAAAGCTG ATCATGAAGC AGACAAAAAA 
CGAAATTTGG AAAATGATGT TAACAGCTTA AAAGATCAAC TTGAAGATTT GAAAAAAAGA 
AATCAAAACT CTCAAATATC CACTGAGAAA GTGAATCAAC TCCAGAGACA ACTGGATGAA 
ACCAATGCTT TACTGCGAAC AGAGTCTGAT ACTGCAGCCC GGTTAAGGAA AACCCAGGCA 
GAAAGTTCAA AACAGATTCA GCAGCTGGAA TCTAACAATA GAGATCTACA AGATAAAAAC 
TGCCTGCTGG AGACTGCCAA GTTAAAACTT GAAAAGGAAT TTATCAATCT TCAGTCAGCT 
CTAGAATCTG AAAGGAGGGA TCGAACCCAT GGATCAGAGA TAATTAATGA TTTACAAGGT 
AGAATATGTG GCCTAGAAGA AGATTTAAAG AACGGCAAAA TCTTACTAGC GAAAGTAGAA 
CTGGAGAAGA GACAACTTCA GGAGAGATTT ACTGATTTGG AAAAGGAAAA AAGCAACATG 
GAAATAGATA TGACATACCA ACTAAAAGTT ATACAGCAGA GCCTAGAACA AGAAGAAGCT 
GAACATAAGG CCACAAAGGC ACGACTAGCA GATAAAAATA AGATCTATGA GTCCATCGAA 
GAAGCCAAAT CAGAAGCCAT GAAAGAAATG GAGAAGAAGC TCTTGGAGGA AAGAACTTTA 
AAACAGAAAG TGGAGAACCT ATTGCTAGAA GCTGAGAAAA GATGTTCTCT ATTAGACTGT 
GACCTCAAAC AGTCACAGCA GAAAATAAAT GAGCTCCTTA AACAGAAAGA TGTGCTAAAT 
GAGGATGTTA GAAACCTGAC ATTAAAAATA GAGCAAGAAA CTCAGAAGCG CTGCCTTACA 
CAAAATGACC TGAAGATGCA AACACAACAG GTTAACACAC TAAAAATGTC AGAAAAGCAG 
TTAAAGCAAG AAAATAACCA TCTCATGGAA ATGAAAATGA ACTTGGAAAA ACAAAATGCT 
GAACTTCGAA AAGAACGTCA GGATGCAGAT GGGCAAATGA AAGAGCTCCA GGATCAGCTC 
GAAGCAGAAC AGTATTTCTC AACCCTTTAT AAAACACAAG TTAGGGAGCT TAAAGAAGAA 
TGTGAAGAAA AGACCAAACT TGGTAAAGAA TTGCAGCAGA AGAAACAGGA ATTACAGGAT 
GAACGGGACT CTTTGGCTGC CCAACTGGAG ATCACCTTGA CCAAAGCAGA TTCTGAGCAA 
CTGGCTCGTT CAATTGCTGA AGAACAATAT TCTGATTTGG AAAAAGAGAA GATCATGAAA 
GAGCTGGAGA TCAAAGAGAT GATGGCTAGA CACAAACAGG AACTTACGGA AAAAGATGCT 
ACAATTGCTT CTCTTGAGGA AACTAATAGG ACACTAACTA GTGATGTTGC CAATCTTGCA 
AATGAGAAAG AAGAATTAAA TAACAAATTG AAAGATGTTC AAGAGCAACT GTCAAGATTG 
AAAGATGAAG AAATAAGCGC AGCAGCTATT AAAGCACAGT TTGAGAAGCA GCTATTAACA 
GAAAGAACAC TCAAAACTCA AGCTGTGAAT AAGTTGGCTG AGATCATGAA TCGAAAAGAA 
CCTGTCAAGC GTGGTAATGA CACAGATGTG CGGAGAAAAG AGAAGGAGAA TAGAAAGCTA 
CATATGGAGC TTAAATCTGA ACGTGAGAAA TTGACCCAGC AGATGATCAA GTATCAGAAA 
GAACTGAATG AAATGCAGGC ACAAATAGCT GAAGAGAGCC AGATTCGAAT TGAACTGCAG 
ATGACATTGG ACAGTAAAGA CAGTGACATT GAGCAGCTGC GGTCACAACT CCAAGCCTTG 
CATATTGGTC TGGATAGTTC CAGTATAGGC AGTGGACCAG GGGATGCTGA GGCAGATGAT 
GGGTTTCCAG AATCAAGATT AGAAGGATGG CTTTCATTGC CTGTACGAAA CAACACTAAG 
AAATTTGGAT GGGTTAAAAA GTATGTGATT GTAAGCAGTA AGAAGATTCT TTTCTATGAC 
AGTGAACAAG ATAAAGAACA ATCCAATCCT TACATGGTTT TAGATATAGA CAAGTTATTT 
CATGTCCGAC CAGTTACACA GACAGATGTG TATAGAGCAG ATGCTAAAGA AATTCCAAGG 
ATATTCCAGA TTCTGTATGC CAATGAAGGA GAAAGTAAGA AGGAACAAGA ATTTCCAGTG 
GAGCCAGTTG GAGAAAAATC TAATTATATT TGCCACAAGG GACATGAGTT TATTCCTACT 
CTTTATCATT TCCCAACCAA CTGTGAGGCT TGTATGAAGC CCCTGTGGCA CATGTTTAAG 
CCTCCTCCTG CTTTGGAGTG CCGCCGTTGC CATATTAAGT GTCATAAAGA TCATATGGAC 
AAAAAGGAGG AGATTATAGC ACCTTGCAAA GTATATTATG ATATTTCAAC GGCAAAGAAT 
CTGTTATTAC TAGCAAATTC TACAGAAGAG CAGCAGAAGT GGGTTAGTCG GTTGGTGAAA 
AAGATACCTA AAAAGCCCCC AGCTCCAGAC CCTTTTGCCC GATCATCTCC TAGAACTTCA 
ATGAAGATAC AGCAAAACCA GTCTATTAGA CGGCCAAGTC GACAGCTTGC CCCAAACAAA 
CCTAGATTGC TTGATTTGGC ATTTAAAGAC TGGGATTGGT CATTTGATGA TGTTGATGGT 
GGTGGTGATG ATGATGACGA TGTTTTTGAT TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508719.1
CDS	207..4202
Translation	MKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGFYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDAEISKHAKNLICAFLTDREVRLGRNGVEEIRQHPFFKNDQWHWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYRENLLLSDSPSCRETDSIQSRKNEESQEIQKKLYTLEEHLSNEMQAKEELEQKCKSVNTRLEKTAKELEEEITLRKSVESALRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQNSQISTEKVNQLQRQLDETNALLRTESDTAARLRKTQAESSKQIQQLESNNRDLQDKNCLLETAKLKLEKEFINLQSALESERRDRTHGSEIINDLQGRICGLEEDLKNGKILLAKVELEKRQLQERFTDLEKEKSNMEIDMTYQLKVIQQSLEQEEAEHKATKARLADKNKIYESIEEAKSEAMKEMEKKLLEERTLKQKVENLLLEAEKRCSLLDCDLKQSQQKINELLKQKDVLNEDVRNLTLKIEQETQKRCLTQNDLKMQTQQVNTLKMSEKQLKQENNHLMEMKMNLEKQNAELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEECEEKTKLGKELQQKKQELQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELTEKDATIASLEETNRTLTSDVANLANEKEELNNKLKDVQEQLSRLKDEEISAAAIKAQFEKQLLTERTLKTQAVNKLAEIMNRKEPVKRGNDTDVRRKEKENRKLHMELKSEREKLTQQMIKYQKELNEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQALHIGLDSSSIGSGPGDAEADDGFPESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEQEFPVEPVGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISTAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPRLLDLAFKDWDWSFDDVDGGGDDDDDVFDF

Target ORF information:

RefSeq Version	XM_011510417.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510417.2

ORF Insert Sequence:

ATGAAGGCAG AAGACTATGA TGTTGTAAAA GTTATTGGAA GAGGTGCTTT TGGTGAAGTG 
CAGTTGGTTC GTCACAAGGC ATCGCAGAAG GTTTATGCTA TGAAGCTTCT TAGTAAGTTT 
GAAATGATAA AAAGATCAGA TTCTGCCTTT TTTTGGGAAG AAAGAGATAT TATGGCCTTT 
GCCAATAGCC CCTGGGTGGT TCAGCTTTTT TATGCCTTTC AAGATGATAG GTATCTGTAC 
ATGGTAATGG AGTACATGCC TGGTGGAGAC CTTGTAAACC TTATGAGTAA TTATGATGTG 
CCTGAAAAAT GGGCCAAATT TTACACTGCT GAAGTTGTTC TTGCTCTGGA TGCAATACAC 
TCCATGGGTT TAATACACAG AGATGTGAAG CCTGACAACA TGCTCTTGGA TAAACATGGA 
CATCTAAAAT TAGCAGATTT TGGCACGTGT ATGAAGATGG ATGAAACAGG CATGGTACAT 
TGTGATACAG CAGTTGGAAC ACCGGATTAT ATATCACCTG AGGTTCTGAA ATCACAAGGG 
GGTGATGGTT TCTATGGGCG AGAATGTGAT TGGTGGTCTG TAGGTGTTTT CCTTTATGAG 
ATGCTAGTGG GGGATACTCC ATTTTATGCG GATTCACTTG TAGGAACATA TAGCAAAATT 
ATGGATCATA AGAATTCACT GTGTTTCCCT GAAGATGCAG AAATTTCCAA ACATGCAAAG 
AATCTCATCT GTGCTTTCTT AACAGATAGG GAGGTACGAC TTGGGAGAAA TGGGGTGGAA 
GAAATCAGAC AGCATCCTTT CTTTAAGAAT GATCAGTGGC ATTGGGATAA CATAAGAGAA 
ACGGCAGCTC CTGTAGTACC TGAACTCAGC AGTGACATAG ACAGCAGCAA TTTCGATGAC 
ATTGAAGATG ACAAAGGAGA TGTAGAAACC TTCCCAATTC CTAAAGCTTT TGTTGGAAAT 
CAGCTGCCTT TCATCGGATT TACCTACTAT AGAGAAAATT TATTATTAAG TGACTCTCCA 
TCTTGTAGAG AAACTGATTC CATACAATCA AGGAAAAATG AAGAAAGTCA AGAGATTCAG 
AAAAAACTGT ATACATTAGA AGAACATCTT AGCAATGAGA TGCAAGCCAA AGAGGAACTG 
GAACAGAAGT GCAAATCTGT TAATACTCGC CTAGAAAAAA CAGCAAAGGA GCTAGAAGAG 
GAGATTACCT TACGGAAAAG TGTGGAATCA GCATTAAGAC AGTTAGAAAG AGAAAAGGCG 
CTTCTTCAGC ACAAAAATGC AGAATATCAG AGGAAAGCTG ATCATGAAGC AGACAAAAAA 
CGAAATTTGG AAAATGATGT TAACAGCTTA AAAGATCAAC TTGAAGATTT GAAAAAAAGA 
AATCAAAACT CTCAAATATC CACTGAGAAA GTGAATCAAC TCCAGAGACA ACTGGATGAA 
ACCAATGCTT TACTGCGAAC AGAGTCTGAT ACTGCAGCCC GGTTAAGGAA AACCCAGGCA 
GAAAGTTCAA AACAGATTCA GCAGCTGGAA TCTAACAATA GAGATCTACA AGATAAAAAC 
TGCCTGCTGG AGACTGCCAA GTTAAAACTT GAAAAGGAAT TTATCAATCT TCAGTCAGCT 
CTAGAATCTG AAAGGAGGGA TCGAACCCAT GGATCAGAGA TAATTAATGA TTTACAAGGT 
AGAATATGTG GCCTAGAAGA AGATTTAAAG AACGGCAAAA TCTTACTAGC GAAAGTAGAA 
CTGGAGAAGA GACAACTTCA GGAGAGATTT ACTGATTTGG AAAAGGAAAA AAGCAACATG 
GAAATAGATA TGACATACCA ACTAAAAGTT ATACAGCAGA GCCTAGAACA AGAAGAAGCT 
GAACATAAGG CCACAAAGGC ACGACTAGCA GATAAAAATA AGATCTATGA GTCCATCGAA 
GAAGCCAAAT CAGAAGCCAT GAAAGAAATG GAGAAGAAGC TCTTGGAGGA AAGAACTTTA 
AAACAGAAAG TGGAGAACCT ATTGCTAGAA GCTGAGAAAA GATGTTCTCT ATTAGACTGT 
GACCTCAAAC AGTCACAGCA GAAAATAAAT GAGCTCCTTA AACAGAAAGA TGTGCTAAAT 
GAGGATGTTA GAAACCTGAC ATTAAAAATA GAGCAAGAAA CTCAGAAGCG CTGCCTTACA 
CAAAATGACC TGAAGATGCA AACACAACAG GTTAACACAC TAAAAATGTC AGAAAAGCAG 
TTAAAGCAAG AAAATAACCA TCTCATGGAA ATGAAAATGA ACTTGGAAAA ACAAAATGCT 
GAACTTCGAA AAGAACGTCA GGATGCAGAT GGGCAAATGA AAGAGCTCCA GGATCAGCTC 
GAAGCAGAAC AGTATTTCTC AACCCTTTAT AAAACACAAG TTAGGGAGCT TAAAGAAGAA 
TGTGAAGAAA AGACCAAACT TGGTAAAGAA TTGCAGCAGA AGAAACAGGA ATTACAGGAT 
GAACGGGACT CTTTGGCTGC CCAACTGGAG ATCACCTTGA CCAAAGCAGA TTCTGAGCAA 
CTGGCTCGTT CAATTGCTGA AGAACAATAT TCTGATTTGG AAAAAGAGAA GATCATGAAA 
GAGCTGGAGA TCAAAGAGAT GATGGCTAGA CACAAACAGG AACTTACGGA AAAAGATGCT 
ACAATTGCTT CTCTTGAGGA AACTAATAGG ACACTAACTA GTGATGTTGC CAATCTTGCA 
AATGAGAAAG AAGAATTAAA TAACAAATTG AAAGATGTTC AAGAGCAACT GTCAAGATTG 
AAAGATGAAG AAATAAGCGC AGCAGCTATT AAAGCACAGT TTGAGAAGCA GCTATTAACA 
GAAAGAACAC TCAAAACTCA AGCTGTGAAT AAGTTGGCTG AGATCATGAA TCGAAAAGAA 
CCTGTCAAGC GTGGTAATGA CACAGATGTG CGGAGAAAAG AGAAGGAGAA TAGAAAGCTA 
CATATGGAGC TTAAATCTGA ACGTGAGAAA TTGACCCAGC AGATGATCAA GTATCAGAAA 
GAACTGAATG AAATGCAGGC ACAAATAGCT GAAGAGAGCC AGATTCGAAT TGAACTGCAG 
ATGACATTGG ACAGTAAAGA CAGTGACATT GAGCAGCTGC GGTCACAACT CCAAGCCTTG 
CATATTGGTC TGGATAGTTC CAGTATAGGC AGTGGACCAG GGGATGCTGA GGCAGATGAT 
GGGTTTCCAG AATCAAGATT AGAAGGATGG CTTTCATTGC CTGTACGAAA CAACACTAAG 
AAATTTGGAT GGGTTAAAAA GTATGTGATT GTAAGCAGTA AGAAGATTCT TTTCTATGAC 
AGTGAACAAG ATAAAGAACA ATCCAATCCT TACATGGTTT TAGATATAGA CAAGTTATTT 
CATGTCCGAC CAGTTACACA GACAGATGTG TATAGAGCAG ATGCTAAAGA AATTCCAAGG 
ATATTCCAGA TTCTGTATGC CAATGAAGGA GAAAGTAAGA AGGAACAAGA ATTTCCAGTG 
GAGCCAGTTG GAGAAAAATC TAATTATATT TGCCACAAGG GACATGAGTT TATTCCTACT 
CTTTATCATT TCCCAACCAA CTGTGAGGCT TGTATGAAGC CCCTGTGGCA CATGTTTAAG 
CCTCCTCCTG CTTTGGAGTG CCGCCGTTGC CATATTAAGT GTCATAAAGA TCATATGGAC 
AAAAAGGAGG AGATTATAGC ACCTTGCAAA GTATATTATG ATATTTCAAC GGCAAAGAAT 
CTGTTATTAC TAGCAAATTC TACAGAAGAG CAGCAGAAGT GGGTTAGTCG GTTGGTGAAA 
AAGATACCTA AAAAGCCCCC AGCTCCAGAC CCTTTTGCCC GATCATCTCC TAGAACTTCA 
ATGAAGATAC AGCAAAACCA GTCTATTAGA CGGCCAAGTC GACAGCTTGC CCCAAACAAA 
CCTAGATTGC TTGATTTGGC ATTTAAAGAC TGGGATTGGT CATTTGATGA TGTTGATGGT 
GGTGGTGATG ATGATGACGA TGTTTTTGAT TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROCK2 ( XM_017005379.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860868.1 Homo sapiens Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X3, mRNA.

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CloneID	OHu62859
Clone ID Related Accession (Same CDS sequence)	XM_017005379.1 , XM_011510417.2 , XM_017005379.2
Accession Version	XM_017005379.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rho-associated protein kinase 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017005379.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCAG AAGACTATGA TGTTGTAAAA GTTATTGGAA GAGGTGCTTT TGGTGAAGTG 
CAGTTGGTTC GTCACAAGGC ATCGCAGAAG GTTTATGCTA TGAAGCTTCT TAGTAAGTTT 
GAAATGATAA AAAGATCAGA TTCTGCCTTT TTTTGGGAAG AAAGAGATAT TATGGCCTTT 
GCCAATAGCC CCTGGGTGGT TCAGCTTTTT TATGCCTTTC AAGATGATAG GTATCTGTAC 
ATGGTAATGG AGTACATGCC TGGTGGAGAC CTTGTAAACC TTATGAGTAA TTATGATGTG 
CCTGAAAAAT GGGCCAAATT TTACACTGCT GAAGTTGTTC TTGCTCTGGA TGCAATACAC 
TCCATGGGTT TAATACACAG AGATGTGAAG CCTGACAACA TGCTCTTGGA TAAACATGGA 
CATCTAAAAT TAGCAGATTT TGGCACGTGT ATGAAGATGG ATGAAACAGG CATGGTACAT 
TGTGATACAG CAGTTGGAAC ACCGGATTAT ATATCACCTG AGGTTCTGAA ATCACAAGGG 
GGTGATGGTT TCTATGGGCG AGAATGTGAT TGGTGGTCTG TAGGTGTTTT CCTTTATGAG 
ATGCTAGTGG GGGATACTCC ATTTTATGCG GATTCACTTG TAGGAACATA TAGCAAAATT 
ATGGATCATA AGAATTCACT GTGTTTCCCT GAAGATGCAG AAATTTCCAA ACATGCAAAG 
AATCTCATCT GTGCTTTCTT AACAGATAGG GAGGTACGAC TTGGGAGAAA TGGGGTGGAA 
GAAATCAGAC AGCATCCTTT CTTTAAGAAT GATCAGTGGC ATTGGGATAA CATAAGAGAA 
ACGGCAGCTC CTGTAGTACC TGAACTCAGC AGTGACATAG ACAGCAGCAA TTTCGATGAC 
ATTGAAGATG ACAAAGGAGA TGTAGAAACC TTCCCAATTC CTAAAGCTTT TGTTGGAAAT 
CAGCTGCCTT TCATCGGATT TACCTACTAT AGAGAAAATT TATTATTAAG TGACTCTCCA 
TCTTGTAGAG AAACTGATTC CATACAATCA AGGAAAAATG AAGAAAGTCA AGAGATTCAG 
AAAAAACTGT ATACATTAGA AGAACATCTT AGCAATGAGA TGCAAGCCAA AGAGGAACTG 
GAACAGAAGT GCAAATCTGT TAATACTCGC CTAGAAAAAA CAGCAAAGGA GCTAGAAGAG 
GAGATTACCT TACGGAAAAG TGTGGAATCA GCATTAAGAC AGTTAGAAAG AGAAAAGGCG 
CTTCTTCAGC ACAAAAATGC AGAATATCAG AGGAAAGCTG ATCATGAAGC AGACAAAAAA 
CGAAATTTGG AAAATGATGT TAACAGCTTA AAAGATCAAC TTGAAGATTT GAAAAAAAGA 
AATCAAAACT CTCAAATATC CACTGAGAAA GTGAATCAAC TCCAGAGACA ACTGGATGAA 
ACCAATGCTT TACTGCGAAC AGAGTCTGAT ACTGCAGCCC GGTTAAGGAA AACCCAGGCA 
GAAAGTTCAA AACAGATTCA GCAGCTGGAA TCTAACAATA GAGATCTACA AGATAAAAAC 
TGCCTGCTGG AGACTGCCAA GTTAAAACTT GAAAAGGAAT TTATCAATCT TCAGTCAGCT 
CTAGAATCTG AAAGGAGGGA TCGAACCCAT GGATCAGAGA TAATTAATGA TTTACAAGGT 
AGAATATGTG GCCTAGAAGA AGATTTAAAG AACGGCAAAA TCTTACTAGC GAAAGTAGAA 
CTGGAGAAGA GACAACTTCA GGAGAGATTT ACTGATTTGG AAAAGGAAAA AAGCAACATG 
GAAATAGATA TGACATACCA ACTAAAAGTT ATACAGCAGA GCCTAGAACA AGAAGAAGCT 
GAACATAAGG CCACAAAGGC ACGACTAGCA GATAAAAATA AGATCTATGA GTCCATCGAA 
GAAGCCAAAT CAGAAGCCAT GAAAGAAATG GAGAAGAAGC TCTTGGAGGA AAGAACTTTA 
AAACAGAAAG TGGAGAACCT ATTGCTAGAA GCTGAGAAAA GATGTTCTCT ATTAGACTGT 
GACCTCAAAC AGTCACAGCA GAAAATAAAT GAGCTCCTTA AACAGAAAGA TGTGCTAAAT 
GAGGATGTTA GAAACCTGAC ATTAAAAATA GAGCAAGAAA CTCAGAAGCG CTGCCTTACA 
CAAAATGACC TGAAGATGCA AACACAACAG GTTAACACAC TAAAAATGTC AGAAAAGCAG 
TTAAAGCAAG AAAATAACCA TCTCATGGAA ATGAAAATGA ACTTGGAAAA ACAAAATGCT 
GAACTTCGAA AAGAACGTCA GGATGCAGAT GGGCAAATGA AAGAGCTCCA GGATCAGCTC 
GAAGCAGAAC AGTATTTCTC AACCCTTTAT AAAACACAAG TTAGGGAGCT TAAAGAAGAA 
TGTGAAGAAA AGACCAAACT TGGTAAAGAA TTGCAGCAGA AGAAACAGGA ATTACAGGAT 
GAACGGGACT CTTTGGCTGC CCAACTGGAG ATCACCTTGA CCAAAGCAGA TTCTGAGCAA 
CTGGCTCGTT CAATTGCTGA AGAACAATAT TCTGATTTGG AAAAAGAGAA GATCATGAAA 
GAGCTGGAGA TCAAAGAGAT GATGGCTAGA CACAAACAGG AACTTACGGA AAAAGATGCT 
ACAATTGCTT CTCTTGAGGA AACTAATAGG ACACTAACTA GTGATGTTGC CAATCTTGCA 
AATGAGAAAG AAGAATTAAA TAACAAATTG AAAGATGTTC AAGAGCAACT GTCAAGATTG 
AAAGATGAAG AAATAAGCGC AGCAGCTATT AAAGCACAGT TTGAGAAGCA GCTATTAACA 
GAAAGAACAC TCAAAACTCA AGCTGTGAAT AAGTTGGCTG AGATCATGAA TCGAAAAGAA 
CCTGTCAAGC GTGGTAATGA CACAGATGTG CGGAGAAAAG AGAAGGAGAA TAGAAAGCTA 
CATATGGAGC TTAAATCTGA ACGTGAGAAA TTGACCCAGC AGATGATCAA GTATCAGAAA 
GAACTGAATG AAATGCAGGC ACAAATAGCT GAAGAGAGCC AGATTCGAAT TGAACTGCAG 
ATGACATTGG ACAGTAAAGA CAGTGACATT GAGCAGCTGC GGTCACAACT CCAAGCCTTG 
CATATTGGTC TGGATAGTTC CAGTATAGGC AGTGGACCAG GGGATGCTGA GGCAGATGAT 
GGGTTTCCAG AATCAAGATT AGAAGGATGG CTTTCATTGC CTGTACGAAA CAACACTAAG 
AAATTTGGAT GGGTTAAAAA GTATGTGATT GTAAGCAGTA AGAAGATTCT TTTCTATGAC 
AGTGAACAAG ATAAAGAACA ATCCAATCCT TACATGGTTT TAGATATAGA CAAGTTATTT 
CATGTCCGAC CAGTTACACA GACAGATGTG TATAGAGCAG ATGCTAAAGA AATTCCAAGG 
ATATTCCAGA TTCTGTATGC CAATGAAGGA GAAAGTAAGA AGGAACAAGA ATTTCCAGTG 
GAGCCAGTTG GAGAAAAATC TAATTATATT TGCCACAAGG GACATGAGTT TATTCCTACT 
CTTTATCATT TCCCAACCAA CTGTGAGGCT TGTATGAAGC CCCTGTGGCA CATGTTTAAG 
CCTCCTCCTG CTTTGGAGTG CCGCCGTTGC CATATTAAGT GTCATAAAGA TCATATGGAC 
AAAAAGGAGG AGATTATAGC ACCTTGCAAA GTATATTATG ATATTTCAAC GGCAAAGAAT 
CTGTTATTAC TAGCAAATTC TACAGAAGAG CAGCAGAAGT GGGTTAGTCG GTTGGTGAAA 
AAGATACCTA AAAAGCCCCC AGCTCCAGAC CCTTTTGCCC GATCATCTCC TAGAACTTCA 
ATGAAGATAC AGCAAAACCA GTCTATTAGA CGGCCAAGTC GACAGCTTGC CCCAAACAAA 
CCTAGATTGC TTGATTTGGC ATTTAAAGAC TGGGATTGGT CATTTGATGA TGTTGATGGT 
GGTGGTGATG ATGATGACGA TGTTTTTGAT TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860868.1
CDS	609..4604
Translation	MKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGFYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDAEISKHAKNLICAFLTDREVRLGRNGVEEIRQHPFFKNDQWHWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYRENLLLSDSPSCRETDSIQSRKNEESQEIQKKLYTLEEHLSNEMQAKEELEQKCKSVNTRLEKTAKELEEEITLRKSVESALRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQNSQISTEKVNQLQRQLDETNALLRTESDTAARLRKTQAESSKQIQQLESNNRDLQDKNCLLETAKLKLEKEFINLQSALESERRDRTHGSEIINDLQGRICGLEEDLKNGKILLAKVELEKRQLQERFTDLEKEKSNMEIDMTYQLKVIQQSLEQEEAEHKATKARLADKNKIYESIEEAKSEAMKEMEKKLLEERTLKQKVENLLLEAEKRCSLLDCDLKQSQQKINELLKQKDVLNEDVRNLTLKIEQETQKRCLTQNDLKMQTQQVNTLKMSEKQLKQENNHLMEMKMNLEKQNAELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEECEEKTKLGKELQQKKQELQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELTEKDATIASLEETNRTLTSDVANLANEKEELNNKLKDVQEQLSRLKDEEISAAAIKAQFEKQLLTERTLKTQAVNKLAEIMNRKEPVKRGNDTDVRRKEKENRKLHMELKSEREKLTQQMIKYQKELNEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQALHIGLDSSSIGSGPGDAEADDGFPESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEQEFPVEPVGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISTAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPRLLDLAFKDWDWSFDDVDGGGDDDDDVFDF

Target ORF information:

RefSeq Version	XM_017005379.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005379.2

ORF Insert Sequence:

ATGAAGGCAG AAGACTATGA TGTTGTAAAA GTTATTGGAA GAGGTGCTTT TGGTGAAGTG 
CAGTTGGTTC GTCACAAGGC ATCGCAGAAG GTTTATGCTA TGAAGCTTCT TAGTAAGTTT 
GAAATGATAA AAAGATCAGA TTCTGCCTTT TTTTGGGAAG AAAGAGATAT TATGGCCTTT 
GCCAATAGCC CCTGGGTGGT TCAGCTTTTT TATGCCTTTC AAGATGATAG GTATCTGTAC 
ATGGTAATGG AGTACATGCC TGGTGGAGAC CTTGTAAACC TTATGAGTAA TTATGATGTG 
CCTGAAAAAT GGGCCAAATT TTACACTGCT GAAGTTGTTC TTGCTCTGGA TGCAATACAC 
TCCATGGGTT TAATACACAG AGATGTGAAG CCTGACAACA TGCTCTTGGA TAAACATGGA 
CATCTAAAAT TAGCAGATTT TGGCACGTGT ATGAAGATGG ATGAAACAGG CATGGTACAT 
TGTGATACAG CAGTTGGAAC ACCGGATTAT ATATCACCTG AGGTTCTGAA ATCACAAGGG 
GGTGATGGTT TCTATGGGCG AGAATGTGAT TGGTGGTCTG TAGGTGTTTT CCTTTATGAG 
ATGCTAGTGG GGGATACTCC ATTTTATGCG GATTCACTTG TAGGAACATA TAGCAAAATT 
ATGGATCATA AGAATTCACT GTGTTTCCCT GAAGATGCAG AAATTTCCAA ACATGCAAAG 
AATCTCATCT GTGCTTTCTT AACAGATAGG GAGGTACGAC TTGGGAGAAA TGGGGTGGAA 
GAAATCAGAC AGCATCCTTT CTTTAAGAAT GATCAGTGGC ATTGGGATAA CATAAGAGAA 
ACGGCAGCTC CTGTAGTACC TGAACTCAGC AGTGACATAG ACAGCAGCAA TTTCGATGAC 
ATTGAAGATG ACAAAGGAGA TGTAGAAACC TTCCCAATTC CTAAAGCTTT TGTTGGAAAT 
CAGCTGCCTT TCATCGGATT TACCTACTAT AGAGAAAATT TATTATTAAG TGACTCTCCA 
TCTTGTAGAG AAACTGATTC CATACAATCA AGGAAAAATG AAGAAAGTCA AGAGATTCAG 
AAAAAACTGT ATACATTAGA AGAACATCTT AGCAATGAGA TGCAAGCCAA AGAGGAACTG 
GAACAGAAGT GCAAATCTGT TAATACTCGC CTAGAAAAAA CAGCAAAGGA GCTAGAAGAG 
GAGATTACCT TACGGAAAAG TGTGGAATCA GCATTAAGAC AGTTAGAAAG AGAAAAGGCG 
CTTCTTCAGC ACAAAAATGC AGAATATCAG AGGAAAGCTG ATCATGAAGC AGACAAAAAA 
CGAAATTTGG AAAATGATGT TAACAGCTTA AAAGATCAAC TTGAAGATTT GAAAAAAAGA 
AATCAAAACT CTCAAATATC CACTGAGAAA GTGAATCAAC TCCAGAGACA ACTGGATGAA 
ACCAATGCTT TACTGCGAAC AGAGTCTGAT ACTGCAGCCC GGTTAAGGAA AACCCAGGCA 
GAAAGTTCAA AACAGATTCA GCAGCTGGAA TCTAACAATA GAGATCTACA AGATAAAAAC 
TGCCTGCTGG AGACTGCCAA GTTAAAACTT GAAAAGGAAT TTATCAATCT TCAGTCAGCT 
CTAGAATCTG AAAGGAGGGA TCGAACCCAT GGATCAGAGA TAATTAATGA TTTACAAGGT 
AGAATATGTG GCCTAGAAGA AGATTTAAAG AACGGCAAAA TCTTACTAGC GAAAGTAGAA 
CTGGAGAAGA GACAACTTCA GGAGAGATTT ACTGATTTGG AAAAGGAAAA AAGCAACATG 
GAAATAGATA TGACATACCA ACTAAAAGTT ATACAGCAGA GCCTAGAACA AGAAGAAGCT 
GAACATAAGG CCACAAAGGC ACGACTAGCA GATAAAAATA AGATCTATGA GTCCATCGAA 
GAAGCCAAAT CAGAAGCCAT GAAAGAAATG GAGAAGAAGC TCTTGGAGGA AAGAACTTTA 
AAACAGAAAG TGGAGAACCT ATTGCTAGAA GCTGAGAAAA GATGTTCTCT ATTAGACTGT 
GACCTCAAAC AGTCACAGCA GAAAATAAAT GAGCTCCTTA AACAGAAAGA TGTGCTAAAT 
GAGGATGTTA GAAACCTGAC ATTAAAAATA GAGCAAGAAA CTCAGAAGCG CTGCCTTACA 
CAAAATGACC TGAAGATGCA AACACAACAG GTTAACACAC TAAAAATGTC AGAAAAGCAG 
TTAAAGCAAG AAAATAACCA TCTCATGGAA ATGAAAATGA ACTTGGAAAA ACAAAATGCT 
GAACTTCGAA AAGAACGTCA GGATGCAGAT GGGCAAATGA AAGAGCTCCA GGATCAGCTC 
GAAGCAGAAC AGTATTTCTC AACCCTTTAT AAAACACAAG TTAGGGAGCT TAAAGAAGAA 
TGTGAAGAAA AGACCAAACT TGGTAAAGAA TTGCAGCAGA AGAAACAGGA ATTACAGGAT 
GAACGGGACT CTTTGGCTGC CCAACTGGAG ATCACCTTGA CCAAAGCAGA TTCTGAGCAA 
CTGGCTCGTT CAATTGCTGA AGAACAATAT TCTGATTTGG AAAAAGAGAA GATCATGAAA 
GAGCTGGAGA TCAAAGAGAT GATGGCTAGA CACAAACAGG AACTTACGGA AAAAGATGCT 
ACAATTGCTT CTCTTGAGGA AACTAATAGG ACACTAACTA GTGATGTTGC CAATCTTGCA 
AATGAGAAAG AAGAATTAAA TAACAAATTG AAAGATGTTC AAGAGCAACT GTCAAGATTG 
AAAGATGAAG AAATAAGCGC AGCAGCTATT AAAGCACAGT TTGAGAAGCA GCTATTAACA 
GAAAGAACAC TCAAAACTCA AGCTGTGAAT AAGTTGGCTG AGATCATGAA TCGAAAAGAA 
CCTGTCAAGC GTGGTAATGA CACAGATGTG CGGAGAAAAG AGAAGGAGAA TAGAAAGCTA 
CATATGGAGC TTAAATCTGA ACGTGAGAAA TTGACCCAGC AGATGATCAA GTATCAGAAA 
GAACTGAATG AAATGCAGGC ACAAATAGCT GAAGAGAGCC AGATTCGAAT TGAACTGCAG 
ATGACATTGG ACAGTAAAGA CAGTGACATT GAGCAGCTGC GGTCACAACT CCAAGCCTTG 
CATATTGGTC TGGATAGTTC CAGTATAGGC AGTGGACCAG GGGATGCTGA GGCAGATGAT 
GGGTTTCCAG AATCAAGATT AGAAGGATGG CTTTCATTGC CTGTACGAAA CAACACTAAG 
AAATTTGGAT GGGTTAAAAA GTATGTGATT GTAAGCAGTA AGAAGATTCT TTTCTATGAC 
AGTGAACAAG ATAAAGAACA ATCCAATCCT TACATGGTTT TAGATATAGA CAAGTTATTT 
CATGTCCGAC CAGTTACACA GACAGATGTG TATAGAGCAG ATGCTAAAGA AATTCCAAGG 
ATATTCCAGA TTCTGTATGC CAATGAAGGA GAAAGTAAGA AGGAACAAGA ATTTCCAGTG 
GAGCCAGTTG GAGAAAAATC TAATTATATT TGCCACAAGG GACATGAGTT TATTCCTACT 
CTTTATCATT TCCCAACCAA CTGTGAGGCT TGTATGAAGC CCCTGTGGCA CATGTTTAAG 
CCTCCTCCTG CTTTGGAGTG CCGCCGTTGC CATATTAAGT GTCATAAAGA TCATATGGAC 
AAAAAGGAGG AGATTATAGC ACCTTGCAAA GTATATTATG ATATTTCAAC GGCAAAGAAT 
CTGTTATTAC TAGCAAATTC TACAGAAGAG CAGCAGAAGT GGGTTAGTCG GTTGGTGAAA 
AAGATACCTA AAAAGCCCCC AGCTCCAGAC CCTTTTGCCC GATCATCTCC TAGAACTTCA 
ATGAAGATAC AGCAAAACCA GTCTATTAGA CGGCCAAGTC GACAGCTTGC CCCAAACAAA 
CCTAGATTGC TTGATTTGGC ATTTAAAGAC TGGGATTGGT CATTTGATGA TGTTGATGGT 
GGTGGTGATG ATGATGACGA TGTTTTTGAT TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROCK2 ( XM_005246190.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005246247.1 Homo sapiens Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu35229
Clone ID Related Accession (Same CDS sequence)	XM_005246190.3
Accession Version	XM_005246190.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rho-associated protein kinase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005246190.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCGGC CCCCGCCGAC GGGGAAAATG CCCGGCGCCC CCGAGACCGC GCCGGGGGAC 
GGGGCAGGCG CGAGCCGCCA GAGGAAGCTG GAGGCGCTGA TCCGAGACCC TCGCTCCCCC 
ATCAACGTGG AGAGCTTGCT GGATGGCTTA AATTCCTTGG TCCTTGATTT AGATTTTCCT 
GCTTTGAGGA AAAACAAGAA CATAGATAAT TTCTTAAATA GATATGAGAA AATTGTGAAA 
AAAATCAGAG GTCTACAGAT GAAGGCAGAA GACTATGATG TTGTAAAAGT TATTGGAAGA 
GGTGCTTTTG GTGAAGTGCA GTTGGTTCGT CACAAGGCAT CGCAGAAGGT TTATGCTATG 
AAGCTTCTTA GTAAGTTTGA AATGATAAAA AGATCAGATT CTGCCTTTTT TTGGGAAGAA 
AGAGATATTA TGGCCTTTGC CAATAGCCCC TGGGTGGTTC AGCTTTTTTA TGCCTTTCAA 
GATGATAGGT ATCTGTACAT GGTAATGGAG TACATGCCTG GTGGAGACCT TGTAAACCTT 
ATGAGTAATT ATGATGTGCC TGAAAAATGG GCCAAATTTT ACACTGCTGA AGTTGTTCTT 
GCTCTGGATG CAATACACTC CATGGGTTTA ATACACAGAG ATGTGAAGCC TGACAACATG 
CTCTTGGATA AACATGGACA TCTAAAATTA GCAGATTTTG GCACGTGTAT GAAGATGGAT 
GAAACAGGCA TGGTACATTG TGATACAGCA GTTGGAACAC CGGATTATAT ATCACCTGAG 
GTTCTGAAAT CACAAGGGGG TGATGGTTTC TATGGGCGAG AATGTGATTG GTGGTCTGTA 
GGTGTTTTCC TTTATGAGAT GCTAGTGGGG GATACTCCAT TTTATGCGGA TTCACTTGTA 
GGAACATATA GCAAAATTAT GGATCATAAG AATTCACTGT GTTTCCCTGA AGATGCAGAA 
ATTTCCAAAC ATGCAAAGAA TCTCATCTGT GCTTTCTTAA CAGATAGGGA GGTACGACTT 
GGGAGAAATG GGGTGGAAGA AATCAGACAG CATCCTTTCT TTAAGAATGA TCAGTGGCAT 
TGGGATAACA TAAGAGAAAC GGCAGCTCCT GTAGTACCTG AACTCAGCAG TGACATAGAC 
AGCAGCAATT TCGATGACAT TGAAGATGAC AAAGGAGATG TAGAAACCTT CCCAATTCCT 
AAAGCTTTTG TTGGAAATCA GCTGCCTTTC ATCGGATTTA CCTACTATAG AGAAAATTTA 
TTATTAAGTG ACTCTCCATC TTGTAGAGAA ACTGATTCCA TACAATCAAG GAAAAATGAA 
GAAAGTCAAG AGATTCAGAA AAAACTGTAT ACATTAGAAG AACATCTTAG CAATGAGATG 
CAAGCCAAAG AGGAACTGGA ACAGAAGTGC AAATCTGTTA ATACTCGCCT AGAAAAAACA 
GCAAAGGAGC TAGAAGAGGA GATTACCTTA CGGAAAAGTG TGGAATCAGC ATTAAGACAG 
TTAGAAAGAG AAAAGGCGCT TCTTCAGCAC AAAAATGCAG AATATCAGAG GAAAGCTGAT 
CATGAAGCAG ACAAAAAACG AAATTTGGAA AATGATGTTA ACAGCTTAAA AGATCAACTT 
GAAGATTTGA AAAAAAGAAA TCAAAACTCT CAAATATCCA CTGAGAAAGT GAATCAACTC 
CAGAGACAAC TGGATGAAAC CAATGCTTTA CTGCGAACAG AGTCTGATAC TGCAGCCCGG 
TTAAGGAAAA CCCAGGCAGA AAGTTCAAAA CAGATTCAGC AGCTGGAATC TAACAATAGA 
GATCTACAAG ATAAAAACTG CCTGCTGGAG ACTGCCAAGT TAAAACTTGA AAAGGAATTT 
ATCAATCTTC AGTCAGCTCT AGAATCTGAA AGGAGGGATC GAACCCATGG ATCAGAGATA 
ATTAATGATT TACAAGGTAG AATATGTGGC CTAGAAGAAG ATTTAAAGAA CGGCAAAATC 
TTACTAGCGA AAGTAGAACT GGAGAAGAGA CAACTTCAGG AGAGATTTAC TGATTTGGAA 
AAGGAAAAAA GCAACATGGA AATAGATATG ACATACCAAC TAAAAGTTAT ACAGCAGAGC 
CTAGAACAAG AAGAAGCTGA ACATAAGGCC ACAAAGGCAC GACTAGCAGA TAAAAATAAG 
ATCTATGAGT CCATCGAAGA AGCCAAATCA GAAGCCATGA AAGAAATGGA GAAGAAGCTC 
TTGGAGGAAA GAACTTTAAA ACAGAAAGTG GAGAACCTAT TGCTAGAAGC TGAGAAAAGA 
TGTTCTCTAT TAGACTGTGA CCTCAAACAG TCACAGCAGA AAATAAATGA GCTCCTTAAA 
CAGAAAGATG TGCTAAATGA GGATGTTAGA AACCTGACAT TAAAAATAGA GCAAGAAACT 
CAGAAGCGCT GCCTTACACA AAATGACCTG AAGATGCAAA CACAACAGGT TAACACACTA 
AAAATGTCAG AAAAGCAGTT AAAGCAAGAA AATAACCATC TCATGGAAAT GAAAATGAAC 
TTGGAAAAAC AAAATGCTGA ACTTCGAAAA GAACGTCAGG ATGCAGATGG GCAAATGAAA 
GAGCTCCAGG ATCAGCTCGA AGCAGAACAG TATTTCTCAA CCCTTTATAA AACACAAGTT 
AGGGAGCTTA AAGAAGAATG TGAAGAAAAG ACCAAACTTG GTAAAGAATT GCAGCAGAAG 
AAACAGGAAT TACAGGATGA ACGGGACTCT TTGGCTGCCC AACTGGAGAT CACCTTGACC 
AAAGCAGATT CTGAGCAACT GGCTCGTTCA ATTGCTGAAG AACAATATTC TGATTTGGAA 
AAAGAGAAGA TCATGAAAGA GCTGGAGATC AAAGAGATGA TGGCTAGACA CAAACAGGAA 
CTTACGGAAA AAGATGCTAC AATTGCTTCT CTTGAGGAAA CTAATAGGAC ACTAACTAGT 
GATGTTGCCA ATCTTGCAAA TGAGAAAGAA GAATTAAATA ACAAATTGAA AGATGTTCAA 
GAGCAACTGT CAAGATTGAA AGATGAAGAA ATAAGCGCAG CAGCTATTAA AGCACAGTTT 
GAGAAGCAGC TATTAACAGA AAGAACACTC AAAACTCAAG CTGTGAATAA GTTGGCTGAG 
ATCATGAATC GAAAAGAACC TGTCAAGCGT GGTAATGACA CAGATGTGCG GAGAAAAGAG 
AAGGAGAATA GAAAGCTACA TATGGAGCTT AAATCTGAAC GTGAGAAATT GACCCAGCAG 
ATGATCAAGT ATCAGAAAGA ACTGAATGAA ATGCAGGCAC AAATAGCTGA AGAGAGCCAG 
ATTCGAATTG AACTGCAGAT GACATTGGAC AGTAAAGACA GTGACATTGA GCAGCTGCGG 
TCACAACTCC AAGCCTTGCA TATTGGTCTG GATAGTTCCA GTATAGGCAG TGGACCAGGG 
GATGCTGAGG CAGATGATGG GTTTCCAGAA TCAAGATTAG AAGGATGGCT TTCATTGCCT 
GTACGAAACA ACACTAAGAA ATTTGGATGG GTTAAAAAGT ATGTGATTGT AAGCAGTAAG 
AAGATTCTTT TCTATGACAG TGAACAAGAT AAAGAACAAT CCAATCCTTA CATGGTTTTA 
GATATAGACA AGTTATTTCA TGTCCGACCA GTTACACAGA CAGATGTGTA TAGAGCAGAT 
GCTAAAGAAA TTCCAAGGAT ATTCCAGATT CTGTATGCCA ATGAAGGAGA AAGTAAGAAG 
GAACAAGAAT TTCCAGTGGA GCCAGTTGGA GAAAAATCTA ATTATATTTG CCACAAGGGA 
CATGAGTTTA TTCCTACTCT TTATCATTTC CCAACCAACT GTGAGGCTTG TATGAAGCCC 
CTGTGGCACA TGTTTAAGCC TCCTCCTGCT TTGGAGTGCC GCCGTTGCCA TATTAAGTGT 
CATAAAGATC ATATGGACAA AAAGGAGGAG ATTATAGCAC CTTGCAAAGT ATATTATGAT 
ATTTCAACGG CAAAGAATCT GTTATTACTA GCAAATTCTA CAGAAGAGCA GCAGAAGTGG 
GTTAGTCGGT TGGTGAAAAA GATACCTAAA AAGCCCCCAG CTCCAGACCC TTTTGCCCGA 
TCATCTCCTA GAACTTCAAT GAAGATACAG CAAAACCAGT CTATTAGACG GCCAAGTCGA 
CAGCTTGCCC CAAACAAACC TAGATTGCTT GATTTGGCAT TTAAAGACTG GGATTGGTCA 
TTTGATGATG TTGATGGTGG TGGTGATGAT GATGACGATG TTTTTGATTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005246247.1
CDS	484..4737
Translation	MSRPPPTGKMPGAPETAPGDGAGASRQRKLEALIRDPRSPINVESLLDGLNSLVLDLDFPALRKNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGFYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDAEISKHAKNLICAFLTDREVRLGRNGVEEIRQHPFFKNDQWHWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYRENLLLSDSPSCRETDSIQSRKNEESQEIQKKLYTLEEHLSNEMQAKEELEQKCKSVNTRLEKTAKELEEEITLRKSVESALRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQNSQISTEKVNQLQRQLDETNALLRTESDTAARLRKTQAESSKQIQQLESNNRDLQDKNCLLETAKLKLEKEFINLQSALESERRDRTHGSEIINDLQGRICGLEEDLKNGKILLAKVELEKRQLQERFTDLEKEKSNMEIDMTYQLKVIQQSLEQEEAEHKATKARLADKNKIYESIEEAKSEAMKEMEKKLLEERTLKQKVENLLLEAEKRCSLLDCDLKQSQQKINELLKQKDVLNEDVRNLTLKIEQETQKRCLTQNDLKMQTQQVNTLKMSEKQLKQENNHLMEMKMNLEKQNAELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEECEEKTKLGKELQQKKQELQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELTEKDATIASLEETNRTLTSDVANLANEKEELNNKLKDVQEQLSRLKDEEISAAAIKAQFEKQLLTERTLKTQAVNKLAEIMNRKEPVKRGNDTDVRRKEKENRKLHMELKSEREKLTQQMIKYQKELNEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQALHIGLDSSSIGSGPGDAEADDGFPESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEQEFPVEPVGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISTAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPRLLDLAFKDWDWSFDDVDGGGDDDDDVFDF

Target ORF information:

RefSeq Version	XM_005246190.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005246190.3

ORF Insert Sequence:

ATGAGCCGGC CCCCGCCGAC GGGGAAAATG CCCGGCGCCC CCGAGACCGC GCCGGGGGAC 
GGGGCAGGCG CGAGCCGCCA GAGGAAGCTG GAGGCGCTGA TCCGAGACCC TCGCTCCCCC 
ATCAACGTGG AGAGCTTGCT GGATGGCTTA AATTCCTTGG TCCTTGATTT AGATTTTCCT 
GCTTTGAGGA AAAACAAGAA CATAGATAAT TTCTTAAATA GATATGAGAA AATTGTGAAA 
AAAATCAGAG GTCTACAGAT GAAGGCAGAA GACTATGATG TTGTAAAAGT TATTGGAAGA 
GGTGCTTTTG GTGAAGTGCA GTTGGTTCGT CACAAGGCAT CGCAGAAGGT TTATGCTATG 
AAGCTTCTTA GTAAGTTTGA AATGATAAAA AGATCAGATT CTGCCTTTTT TTGGGAAGAA 
AGAGATATTA TGGCCTTTGC CAATAGCCCC TGGGTGGTTC AGCTTTTTTA TGCCTTTCAA 
GATGATAGGT ATCTGTACAT GGTAATGGAG TACATGCCTG GTGGAGACCT TGTAAACCTT 
ATGAGTAATT ATGATGTGCC TGAAAAATGG GCCAAATTTT ACACTGCTGA AGTTGTTCTT 
GCTCTGGATG CAATACACTC CATGGGTTTA ATACACAGAG ATGTGAAGCC TGACAACATG 
CTCTTGGATA AACATGGACA TCTAAAATTA GCAGATTTTG GCACGTGTAT GAAGATGGAT 
GAAACAGGCA TGGTACATTG TGATACAGCA GTTGGAACAC CGGATTATAT ATCACCTGAG 
GTTCTGAAAT CACAAGGGGG TGATGGTTTC TATGGGCGAG AATGTGATTG GTGGTCTGTA 
GGTGTTTTCC TTTATGAGAT GCTAGTGGGG GATACTCCAT TTTATGCGGA TTCACTTGTA 
GGAACATATA GCAAAATTAT GGATCATAAG AATTCACTGT GTTTCCCTGA AGATGCAGAA 
ATTTCCAAAC ATGCAAAGAA TCTCATCTGT GCTTTCTTAA CAGATAGGGA GGTACGACTT 
GGGAGAAATG GGGTGGAAGA AATCAGACAG CATCCTTTCT TTAAGAATGA TCAGTGGCAT 
TGGGATAACA TAAGAGAAAC GGCAGCTCCT GTAGTACCTG AACTCAGCAG TGACATAGAC 
AGCAGCAATT TCGATGACAT TGAAGATGAC AAAGGAGATG TAGAAACCTT CCCAATTCCT 
AAAGCTTTTG TTGGAAATCA GCTGCCTTTC ATCGGATTTA CCTACTATAG AGAAAATTTA 
TTATTAAGTG ACTCTCCATC TTGTAGAGAA ACTGATTCCA TACAATCAAG GAAAAATGAA 
GAAAGTCAAG AGATTCAGAA AAAACTGTAT ACATTAGAAG AACATCTTAG CAATGAGATG 
CAAGCCAAAG AGGAACTGGA ACAGAAGTGC AAATCTGTTA ATACTCGCCT AGAAAAAACA 
GCAAAGGAGC TAGAAGAGGA GATTACCTTA CGGAAAAGTG TGGAATCAGC ATTAAGACAG 
TTAGAAAGAG AAAAGGCGCT TCTTCAGCAC AAAAATGCAG AATATCAGAG GAAAGCTGAT 
CATGAAGCAG ACAAAAAACG AAATTTGGAA AATGATGTTA ACAGCTTAAA AGATCAACTT 
GAAGATTTGA AAAAAAGAAA TCAAAACTCT CAAATATCCA CTGAGAAAGT GAATCAACTC 
CAGAGACAAC TGGATGAAAC CAATGCTTTA CTGCGAACAG AGTCTGATAC TGCAGCCCGG 
TTAAGGAAAA CCCAGGCAGA AAGTTCAAAA CAGATTCAGC AGCTGGAATC TAACAATAGA 
GATCTACAAG ATAAAAACTG CCTGCTGGAG ACTGCCAAGT TAAAACTTGA AAAGGAATTT 
ATCAATCTTC AGTCAGCTCT AGAATCTGAA AGGAGGGATC GAACCCATGG ATCAGAGATA 
ATTAATGATT TACAAGGTAG AATATGTGGC CTAGAAGAAG ATTTAAAGAA CGGCAAAATC 
TTACTAGCGA AAGTAGAACT GGAGAAGAGA CAACTTCAGG AGAGATTTAC TGATTTGGAA 
AAGGAAAAAA GCAACATGGA AATAGATATG ACATACCAAC TAAAAGTTAT ACAGCAGAGC 
CTAGAACAAG AAGAAGCTGA ACATAAGGCC ACAAAGGCAC GACTAGCAGA TAAAAATAAG 
ATCTATGAGT CCATCGAAGA AGCCAAATCA GAAGCCATGA AAGAAATGGA GAAGAAGCTC 
TTGGAGGAAA GAACTTTAAA ACAGAAAGTG GAGAACCTAT TGCTAGAAGC TGAGAAAAGA 
TGTTCTCTAT TAGACTGTGA CCTCAAACAG TCACAGCAGA AAATAAATGA GCTCCTTAAA 
CAGAAAGATG TGCTAAATGA GGATGTTAGA AACCTGACAT TAAAAATAGA GCAAGAAACT 
CAGAAGCGCT GCCTTACACA AAATGACCTG AAGATGCAAA CACAACAGGT TAACACACTA 
AAAATGTCAG AAAAGCAGTT AAAGCAAGAA AATAACCATC TCATGGAAAT GAAAATGAAC 
TTGGAAAAAC AAAATGCTGA ACTTCGAAAA GAACGTCAGG ATGCAGATGG GCAAATGAAA 
GAGCTCCAGG ATCAGCTCGA AGCAGAACAG TATTTCTCAA CCCTTTATAA AACACAAGTT 
AGGGAGCTTA AAGAAGAATG TGAAGAAAAG ACCAAACTTG GTAAAGAATT GCAGCAGAAG 
AAACAGGAAT TACAGGATGA ACGGGACTCT TTGGCTGCCC AACTGGAGAT CACCTTGACC 
AAAGCAGATT CTGAGCAACT GGCTCGTTCA ATTGCTGAAG AACAATATTC TGATTTGGAA 
AAAGAGAAGA TCATGAAAGA GCTGGAGATC AAAGAGATGA TGGCTAGACA CAAACAGGAA 
CTTACGGAAA AAGATGCTAC AATTGCTTCT CTTGAGGAAA CTAATAGGAC ACTAACTAGT 
GATGTTGCCA ATCTTGCAAA TGAGAAAGAA GAATTAAATA ACAAATTGAA AGATGTTCAA 
GAGCAACTGT CAAGATTGAA AGATGAAGAA ATAAGCGCAG CAGCTATTAA AGCACAGTTT 
GAGAAGCAGC TATTAACAGA AAGAACACTC AAAACTCAAG CTGTGAATAA GTTGGCTGAG 
ATCATGAATC GAAAAGAACC TGTCAAGCGT GGTAATGACA CAGATGTGCG GAGAAAAGAG 
AAGGAGAATA GAAAGCTACA TATGGAGCTT AAATCTGAAC GTGAGAAATT GACCCAGCAG 
ATGATCAAGT ATCAGAAAGA ACTGAATGAA ATGCAGGCAC AAATAGCTGA AGAGAGCCAG 
ATTCGAATTG AACTGCAGAT GACATTGGAC AGTAAAGACA GTGACATTGA GCAGCTGCGG 
TCACAACTCC AAGCCTTGCA TATTGGTCTG GATAGTTCCA GTATAGGCAG TGGACCAGGG 
GATGCTGAGG CAGATGATGG GTTTCCAGAA TCAAGATTAG AAGGATGGCT TTCATTGCCT 
GTACGAAACA ACACTAAGAA ATTTGGATGG GTTAAAAAGT ATGTGATTGT AAGCAGTAAG 
AAGATTCTTT TCTATGACAG TGAACAAGAT AAAGAACAAT CCAATCCTTA CATGGTTTTA 
GATATAGACA AGTTATTTCA TGTCCGACCA GTTACACAGA CAGATGTGTA TAGAGCAGAT 
GCTAAAGAAA TTCCAAGGAT ATTCCAGATT CTGTATGCCA ATGAAGGAGA AAGTAAGAAG 
GAACAAGAAT TTCCAGTGGA GCCAGTTGGA GAAAAATCTA ATTATATTTG CCACAAGGGA 
CATGAGTTTA TTCCTACTCT TTATCATTTC CCAACCAACT GTGAGGCTTG TATGAAGCCC 
CTGTGGCACA TGTTTAAGCC TCCTCCTGCT TTGGAGTGCC GCCGTTGCCA TATTAAGTGT 
CATAAAGATC ATATGGACAA AAAGGAGGAG ATTATAGCAC CTTGCAAAGT ATATTATGAT 
ATTTCAACGG CAAAGAATCT GTTATTACTA GCAAATTCTA CAGAAGAGCA GCAGAAGTGG 
GTTAGTCGGT TGGTGAAAAA GATACCTAAA AAGCCCCCAG CTCCAGACCC TTTTGCCCGA 
TCATCTCCTA GAACTTCAAT GAAGATACAG CAAAACCAGT CTATTAGACG GCCAAGTCGA 
CAGCTTGCCC CAAACAAACC TAGATTGCTT GATTTGGCAT TTAAAGACTG GGATTGGTCA 
TTTGATGATG TTGATGGTGG TGGTGATGAT GATGACGATG TTTTTGATTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROCK2 ( XM_017005378.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860867.1 Homo sapiens Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X2, mRNA.

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CloneID	OHu92799
Clone ID Related Accession (Same CDS sequence)	XM_017005378.2 , XM_017005378.1
Accession Version	XM_017005378.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4215bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rho-associated protein kinase 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017005378.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCGGC CCCCGCCGAC GGGGAAAATG CCCGGCGCCC CCGAGACCGC GCCGGGGGAC 
GGGGCAGGCG CGAGCCGCCA GAGGAAGCTG GAGGCGCTGA TCCGAGACCC TCGCTCCCCC 
ATCAACGTGG AGAGCTTGCT GGATGGCTTA AATTCCTTGG TCCTTGATTT AGATTTTCCT 
GCTTTGAGGA AAAACAAGAA CATAGATAAT TTCTTAAATA GATATGAGAA AATTGTGAAA 
AAAATCAGAG GTCTACAGAT GAAGGCAGAA GACTATGATG TTGTAAAAGT TATTGGAAGA 
GGTGCTTTTG GTGAAGTGCA GTTGGTTCGT CACAAGGCAT CGCAGAAGGT TTATGCTATG 
AAGCTTCTTA GTAAGTTTGA AATGATAAAA AGATCAGATT CTGCCTTTTT TTGGGAAGAA 
AGAGATATTA TGGCCTTTGC CAATAGCCCC TGGGTGGTTC AGCTTTTTTA TGCCTTTCAA 
GATGATAGGT ATCTGTACAT GGTAATGGAG TACATGCCTG GTGGAGACCT TGTAAACCTT 
ATGAGTAATT ATGATGTGCC TGAAAAATGG GCCAAATTTT ACACTGCTGA AGTTGTTCTT 
GCTCTGGATG CAATACACTC CATGGGTTTA ATACACAGAG ATGTGAAGCC TGACAACATG 
CTCTTGGATA AACATGGACA TCTAAAATTA GCAGATTTTG GCACGTGTAT GAAGATGGAT 
GAAACAGGCA TGGTACATTG TGATACAGCA GTTGGAACAC CGGATTATAT ATCACCTGAG 
GTTCTGAAAT CACAAGGGGG TGATGGTTTC TATGGGCGAG AATGTGATTG GTGGTCTGTA 
GGTGTTTTCC TTTATGAGAT GCTAGTGGGG GATACTCCAT TTTATGCGGA TTCACTTGTA 
GGAACATATA GCAAAATTAT GGATCATAAG AATTCACTGT GTTTCCCTGA AGATGCAGAA 
ATTTCCAAAC ATGCAAAGAA TCTCATCTGT GCTTTCTTAA CAGATAGGGA GGTACGACTT 
GGGAGAAATG GGGTGGAAGA AATCAGACAG CATCCTTTCT TTAAGAATGA TCAGTGGCAT 
TGGGATAACA TAAGAGAAAC GGCAGCTCCT GTAGTACCTG AACTCAGCAG TGACATAGAC 
AGCAGCAATT TCGATGACAT TGAAGATGAC AAAGGAGATG TAGAAACCTT CCCAATTCCT 
AAAGCTTTTG TTGGAAATCA GCTGCCTTTC ATCGGATTTA CCTACTATAG AGAAAATTTA 
TTATTAAGTG ACTCTCCATC TTGTAGAGAA ACTGATTCCA TACAATCAAG GAAAAATGAA 
GAAAGTCAAG AGATTCAGAA AAAACTGTAT ACATTAGAAG AACATCTTAG CAATGAGATG 
CAAGCCAAAG AGGAACTGGA ACAGAAGTGC AAATCTGTTA ATACTCGCCT AGAAAAAACA 
GCAAAGGAGC TAGAAGAGGA GATTACCTTA CGGAAAAGTG TGGAATCAGC ATTAAGACAG 
TTAGAAAGAG AAAAGGCGCT TCTTCAGCAC AAAAATGCAG AATATCAGAG GAAAGCTGAT 
CATGAAGCAG ACAAAAAACG AAATTTGGAA AATGATGTTA ACAGCTTAAA AGATCAACTT 
GAAGATTTGA AAAAAAGAAA TCAAAACTCT CAAATATCCA CTGAGAAAGT GAATCAACTC 
CAGAGACAAC TGGATGAAAC CAATGCTTTA CTGCGAACAG AGTCTGATAC TGCAGCCCGG 
TTAAGGAAAA CCCAGGCAGA AAGTTCAAAA CAGATTCAGC AGCTGGAATC TAACAATAGA 
GATCTACAAG ATAAAAACTG CCTGCTGGAG ACTGCCAAGT TAAAACTTGA AAAGGAATTT 
ATCAATCTTC AGTCAGCTCT AGAATCTGAA AGGAGGGATC GAACCCATGG ATCAGAGATA 
ATTAATGATT TACAAGGTAG AATATGTGGC CTAGAAGAAG ATTTAAAGAA CGGCAAAATC 
TTACTAGCGA AAGTAGAACT GGAGAAGAGA CAACTTCAGG AGAGATTTAC TGATTTGGAA 
AAGGAAAAAA GCAACATGGA AATAGATATG ACATACCAAC TAAAAGTTAT ACAGCAGAGC 
CTAGAACAAG AAGAAGCTGA ACATAAGGCC ACAAAGGCAC GACTAGCAGA TAAAAATAAG 
ATCTATGAGT CCATCGAAGA AGCCAAATCA GAAGCCATGA AAGAAATGGA GAAGAAGCTC 
TTGGAGGAAA GAACTTTAAA ACAGAAAGTG GAGAACCTAT TGCTAGAAGC TGAGAAAAGA 
TGTTCTCTAT TAGACTGTGA CCTCAAACAG TCACAGCAGA AAATAAATGA GCTCCTTAAA 
CAGAAAGATG TGCTAAATGA GGATGTTAGA AACCTGACAT TAAAAATAGA GCAAGAAACT 
CAGAAGCGCT GCCTTACACA AAATGACCTG AAGATGCAAA CACAACAGGT TAACACACTA 
AAAATGTCAG AAAAGCAGTT AAAGCAAGAA AATAACCATC TCATGGAAAT GAAAATGAAC 
TTGGAAAAAC AAAATGCTGA ACTTCGAAAA GAACGTCAGG ATGCAGATGG GCAAATGAAA 
GAGCTCCAGG ATCAGCTCGA AGCAGAACAG TATTTCTCAA CCCTTTATAA AACACAAGTT 
AGGGAGCTTA AAGAAGAATG TGAAGAAAAG ACCAAACTTG GTAAAGAATT GCAGCAGAAG 
AAACAGGAAT TACAGGATGA ACGGGACTCT TTGGCTGCCC AACTGGAGAT CACCTTGACC 
AAAGCAGATT CTGAGCAACT GGCTCGTTCA ATTGCTGAAG AACAATATTC TGATTTGGAA 
AAAGAGAAGA TCATGAAAGA GCTGGAGATC AAAGAGATGA TGGCTAGACA CAAACAGGAA 
CTTACGGAAA AAGATGCTAC AATTGCTTCT CTTGAGGAAA CTAATAGGAC ACTAACTAGT 
GATGTTGCCA ATCTTGCAAA TGAGAAAGAA GAATTAAATA ACAAATTGAA AGATGTTCAA 
GAGCAACTGT CAAGATTGAA AGATGAAGAA ATAAGCGCAG CAGCTATTAA AGCACAGTTT 
GAGAAGCAGC TATTAACAGA AAGAACACTC AAAACTCAAG CTGTGAATAA GTTGGCTGAG 
ATCATGAATC GAAAAGAACC TGTCAAGCGT GGTAATGACA CAGATGTGCG GAGAAAAGAG 
AAGGAGAATA GAAAGCTACA TATGGAGCTT AAATCTGAAC GTGAGAAATT GACCCAGCAG 
ATGATCAAGT ATCAGAAAGA ACTGAATGAA ATGCAGGCAC AAATAGCTGA AGAGAGCCAG 
ATTCGAATTG AACTGCAGAT GACATTGGAC AGTAAAGACA GTGACATTGA GCAGCTGCGG 
TCACAACTCC AAGCCTTGCA TATTGGTCTG GATAGTTCCA GTATAGGCAG TGGACCAGGG 
GATGCTGAGG CAGATGATGG GTTTCCAGAA TCAAGATTAG AAGGATGGCT TTCATTGCCT 
GTACGAAACA ACACTAAGAA ATTTGGATGG GTTAAAAAGT ATGTGATTGT AAGCAGTAAG 
AAGATTCTTT TCTATGACAG TGAACAAGAT AAAGAACAAT CCAATCCTTA CATGGTTTTA 
GATATAGACA AGTTATTTCA TGTCCGACCA GTTACACAGA CAGATGTGTA TAGAGCAGAT 
GCTAAAGAAA TTCCAAGGAT ATTCCAGATT CTGTATGCCA ATGAAGGAGA AAGTAAGAAG 
GAACAAGAAT TTCCAGTGGA GCCAGTTGGA GAAAAATCTA ATTATATTTG CCACAAGGGA 
CATGAGTTTA TTCCTACTCT TTATCATTTC CCAACCAACT GTGAGGCTTG TATGAAGCCC 
CTGTGGCACA TGTTTAAGCC TCCTCCTGCT TTGGAGTGCC GCCGTTGCCA TATTAAGTGT 
CATAAAGATC ATATGGACAA AAAGGAGGAG ATTATAGCAC CTTGCAAAGT ATATTATGAT 
ATTTCAACGG CAAAGAATCT GTTATTACTA GCAAATTCTA CAGAAGAGCA GCAGAAGTGG 
GTTAGTCGGT TGGTGAAAAA GATACCTAAA AAGCCCCCAG CTCCAGACCC TTTTGCCCGA 
TCATCTCCTA GAACTTCAAT GAAGATACAG CAAAACCAGT CTATTAGACG GCCAAGTCGA 
CAGCTTGCCC CAAACAAACC TAGGGATGAA CAGAGCGCTT GGGGAAACGA GGGAAGCATG 
GAGAATTCTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860867.1
CDS	340..4554
Translation	MSRPPPTGKMPGAPETAPGDGAGASRQRKLEALIRDPRSPINVESLLDGLNSLVLDLDFPALRKNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGFYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDAEISKHAKNLICAFLTDREVRLGRNGVEEIRQHPFFKNDQWHWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYRENLLLSDSPSCRETDSIQSRKNEESQEIQKKLYTLEEHLSNEMQAKEELEQKCKSVNTRLEKTAKELEEEITLRKSVESALRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQNSQISTEKVNQLQRQLDETNALLRTESDTAARLRKTQAESSKQIQQLESNNRDLQDKNCLLETAKLKLEKEFINLQSALESERRDRTHGSEIINDLQGRICGLEEDLKNGKILLAKVELEKRQLQERFTDLEKEKSNMEIDMTYQLKVIQQSLEQEEAEHKATKARLADKNKIYESIEEAKSEAMKEMEKKLLEERTLKQKVENLLLEAEKRCSLLDCDLKQSQQKINELLKQKDVLNEDVRNLTLKIEQETQKRCLTQNDLKMQTQQVNTLKMSEKQLKQENNHLMEMKMNLEKQNAELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEECEEKTKLGKELQQKKQELQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELTEKDATIASLEETNRTLTSDVANLANEKEELNNKLKDVQEQLSRLKDEEISAAAIKAQFEKQLLTERTLKTQAVNKLAEIMNRKEPVKRGNDTDVRRKEKENRKLHMELKSEREKLTQQMIKYQKELNEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQALHIGLDSSSIGSGPGDAEADDGFPESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEQEFPVEPVGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISTAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPRDEQSAWGNEGSMENSA

Target ORF information:

RefSeq Version	XM_017005378.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005378.2

ORF Insert Sequence:

ATGAGCCGGC CCCCGCCGAC GGGGAAAATG CCCGGCGCCC CCGAGACCGC GCCGGGGGAC 
GGGGCAGGCG CGAGCCGCCA GAGGAAGCTG GAGGCGCTGA TCCGAGACCC TCGCTCCCCC 
ATCAACGTGG AGAGCTTGCT GGATGGCTTA AATTCCTTGG TCCTTGATTT AGATTTTCCT 
GCTTTGAGGA AAAACAAGAA CATAGATAAT TTCTTAAATA GATATGAGAA AATTGTGAAA 
AAAATCAGAG GTCTACAGAT GAAGGCAGAA GACTATGATG TTGTAAAAGT TATTGGAAGA 
GGTGCTTTTG GTGAAGTGCA GTTGGTTCGT CACAAGGCAT CGCAGAAGGT TTATGCTATG 
AAGCTTCTTA GTAAGTTTGA AATGATAAAA AGATCAGATT CTGCCTTTTT TTGGGAAGAA 
AGAGATATTA TGGCCTTTGC CAATAGCCCC TGGGTGGTTC AGCTTTTTTA TGCCTTTCAA 
GATGATAGGT ATCTGTACAT GGTAATGGAG TACATGCCTG GTGGAGACCT TGTAAACCTT 
ATGAGTAATT ATGATGTGCC TGAAAAATGG GCCAAATTTT ACACTGCTGA AGTTGTTCTT 
GCTCTGGATG CAATACACTC CATGGGTTTA ATACACAGAG ATGTGAAGCC TGACAACATG 
CTCTTGGATA AACATGGACA TCTAAAATTA GCAGATTTTG GCACGTGTAT GAAGATGGAT 
GAAACAGGCA TGGTACATTG TGATACAGCA GTTGGAACAC CGGATTATAT ATCACCTGAG 
GTTCTGAAAT CACAAGGGGG TGATGGTTTC TATGGGCGAG AATGTGATTG GTGGTCTGTA 
GGTGTTTTCC TTTATGAGAT GCTAGTGGGG GATACTCCAT TTTATGCGGA TTCACTTGTA 
GGAACATATA GCAAAATTAT GGATCATAAG AATTCACTGT GTTTCCCTGA AGATGCAGAA 
ATTTCCAAAC ATGCAAAGAA TCTCATCTGT GCTTTCTTAA CAGATAGGGA GGTACGACTT 
GGGAGAAATG GGGTGGAAGA AATCAGACAG CATCCTTTCT TTAAGAATGA TCAGTGGCAT 
TGGGATAACA TAAGAGAAAC GGCAGCTCCT GTAGTACCTG AACTCAGCAG TGACATAGAC 
AGCAGCAATT TCGATGACAT TGAAGATGAC AAAGGAGATG TAGAAACCTT CCCAATTCCT 
AAAGCTTTTG TTGGAAATCA GCTGCCTTTC ATCGGATTTA CCTACTATAG AGAAAATTTA 
TTATTAAGTG ACTCTCCATC TTGTAGAGAA ACTGATTCCA TACAATCAAG GAAAAATGAA 
GAAAGTCAAG AGATTCAGAA AAAACTGTAT ACATTAGAAG AACATCTTAG CAATGAGATG 
CAAGCCAAAG AGGAACTGGA ACAGAAGTGC AAATCTGTTA ATACTCGCCT AGAAAAAACA 
GCAAAGGAGC TAGAAGAGGA GATTACCTTA CGGAAAAGTG TGGAATCAGC ATTAAGACAG 
TTAGAAAGAG AAAAGGCGCT TCTTCAGCAC AAAAATGCAG AATATCAGAG GAAAGCTGAT 
CATGAAGCAG ACAAAAAACG AAATTTGGAA AATGATGTTA ACAGCTTAAA AGATCAACTT 
GAAGATTTGA AAAAAAGAAA TCAAAACTCT CAAATATCCA CTGAGAAAGT GAATCAACTC 
CAGAGACAAC TGGATGAAAC CAATGCTTTA CTGCGAACAG AGTCTGATAC TGCAGCCCGG 
TTAAGGAAAA CCCAGGCAGA AAGTTCAAAA CAGATTCAGC AGCTGGAATC TAACAATAGA 
GATCTACAAG ATAAAAACTG CCTGCTGGAG ACTGCCAAGT TAAAACTTGA AAAGGAATTT 
ATCAATCTTC AGTCAGCTCT AGAATCTGAA AGGAGGGATC GAACCCATGG ATCAGAGATA 
ATTAATGATT TACAAGGTAG AATATGTGGC CTAGAAGAAG ATTTAAAGAA CGGCAAAATC 
TTACTAGCGA AAGTAGAACT GGAGAAGAGA CAACTTCAGG AGAGATTTAC TGATTTGGAA 
AAGGAAAAAA GCAACATGGA AATAGATATG ACATACCAAC TAAAAGTTAT ACAGCAGAGC 
CTAGAACAAG AAGAAGCTGA ACATAAGGCC ACAAAGGCAC GACTAGCAGA TAAAAATAAG 
ATCTATGAGT CCATCGAAGA AGCCAAATCA GAAGCCATGA AAGAAATGGA GAAGAAGCTC 
TTGGAGGAAA GAACTTTAAA ACAGAAAGTG GAGAACCTAT TGCTAGAAGC TGAGAAAAGA 
TGTTCTCTAT TAGACTGTGA CCTCAAACAG TCACAGCAGA AAATAAATGA GCTCCTTAAA 
CAGAAAGATG TGCTAAATGA GGATGTTAGA AACCTGACAT TAAAAATAGA GCAAGAAACT 
CAGAAGCGCT GCCTTACACA AAATGACCTG AAGATGCAAA CACAACAGGT TAACACACTA 
AAAATGTCAG AAAAGCAGTT AAAGCAAGAA AATAACCATC TCATGGAAAT GAAAATGAAC 
TTGGAAAAAC AAAATGCTGA ACTTCGAAAA GAACGTCAGG ATGCAGATGG GCAAATGAAA 
GAGCTCCAGG ATCAGCTCGA AGCAGAACAG TATTTCTCAA CCCTTTATAA AACACAAGTT 
AGGGAGCTTA AAGAAGAATG TGAAGAAAAG ACCAAACTTG GTAAAGAATT GCAGCAGAAG 
AAACAGGAAT TACAGGATGA ACGGGACTCT TTGGCTGCCC AACTGGAGAT CACCTTGACC 
AAAGCAGATT CTGAGCAACT GGCTCGTTCA ATTGCTGAAG AACAATATTC TGATTTGGAA 
AAAGAGAAGA TCATGAAAGA GCTGGAGATC AAAGAGATGA TGGCTAGACA CAAACAGGAA 
CTTACGGAAA AAGATGCTAC AATTGCTTCT CTTGAGGAAA CTAATAGGAC ACTAACTAGT 
GATGTTGCCA ATCTTGCAAA TGAGAAAGAA GAATTAAATA ACAAATTGAA AGATGTTCAA 
GAGCAACTGT CAAGATTGAA AGATGAAGAA ATAAGCGCAG CAGCTATTAA AGCACAGTTT 
GAGAAGCAGC TATTAACAGA AAGAACACTC AAAACTCAAG CTGTGAATAA GTTGGCTGAG 
ATCATGAATC GAAAAGAACC TGTCAAGCGT GGTAATGACA CAGATGTGCG GAGAAAAGAG 
AAGGAGAATA GAAAGCTACA TATGGAGCTT AAATCTGAAC GTGAGAAATT GACCCAGCAG 
ATGATCAAGT ATCAGAAAGA ACTGAATGAA ATGCAGGCAC AAATAGCTGA AGAGAGCCAG 
ATTCGAATTG AACTGCAGAT GACATTGGAC AGTAAAGACA GTGACATTGA GCAGCTGCGG 
TCACAACTCC AAGCCTTGCA TATTGGTCTG GATAGTTCCA GTATAGGCAG TGGACCAGGG 
GATGCTGAGG CAGATGATGG GTTTCCAGAA TCAAGATTAG AAGGATGGCT TTCATTGCCT 
GTACGAAACA ACACTAAGAA ATTTGGATGG GTTAAAAAGT ATGTGATTGT AAGCAGTAAG 
AAGATTCTTT TCTATGACAG TGAACAAGAT AAAGAACAAT CCAATCCTTA CATGGTTTTA 
GATATAGACA AGTTATTTCA TGTCCGACCA GTTACACAGA CAGATGTGTA TAGAGCAGAT 
GCTAAAGAAA TTCCAAGGAT ATTCCAGATT CTGTATGCCA ATGAAGGAGA AAGTAAGAAG 
GAACAAGAAT TTCCAGTGGA GCCAGTTGGA GAAAAATCTA ATTATATTTG CCACAAGGGA 
CATGAGTTTA TTCCTACTCT TTATCATTTC CCAACCAACT GTGAGGCTTG TATGAAGCCC 
CTGTGGCACA TGTTTAAGCC TCCTCCTGCT TTGGAGTGCC GCCGTTGCCA TATTAAGTGT 
CATAAAGATC ATATGGACAA AAAGGAGGAG ATTATAGCAC CTTGCAAAGT ATATTATGAT 
ATTTCAACGG CAAAGAATCT GTTATTACTA GCAAATTCTA CAGAAGAGCA GCAGAAGTGG 
GTTAGTCGGT TGGTGAAAAA GATACCTAAA AAGCCCCCAG CTCCAGACCC TTTTGCCCGA 
TCATCTCCTA GAACTTCAAT GAAGATACAG CAAAACCAGT CTATTAGACG GCCAAGTCGA 
CAGCTTGCCC CAAACAAACC TAGGGATGAA CAGAGCGCTT GGGGAAACGA GGGAAGCATG 
GAGAATTCTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROCK2 ( XM_017005378.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860867.1 Homo sapiens Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X2, mRNA.

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CloneID	OHu92799
Clone ID Related Accession (Same CDS sequence)	XM_017005378.2 , XM_017005378.1
Accession Version	XM_017005378.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4215bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rho-associated protein kinase 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCGGC CCCCGCCGAC GGGGAAAATG CCCGGCGCCC CCGAGACCGC GCCGGGGGAC 
GGGGCAGGCG CGAGCCGCCA GAGGAAGCTG GAGGCGCTGA TCCGAGACCC TCGCTCCCCC 
ATCAACGTGG AGAGCTTGCT GGATGGCTTA AATTCCTTGG TCCTTGATTT AGATTTTCCT 
GCTTTGAGGA AAAACAAGAA CATAGATAAT TTCTTAAATA GATATGAGAA AATTGTGAAA 
AAAATCAGAG GTCTACAGAT GAAGGCAGAA GACTATGATG TTGTAAAAGT TATTGGAAGA 
GGTGCTTTTG GTGAAGTGCA GTTGGTTCGT CACAAGGCAT CGCAGAAGGT TTATGCTATG 
AAGCTTCTTA GTAAGTTTGA AATGATAAAA AGATCAGATT CTGCCTTTTT TTGGGAAGAA 
AGAGATATTA TGGCCTTTGC CAATAGCCCC TGGGTGGTTC AGCTTTTTTA TGCCTTTCAA 
GATGATAGGT ATCTGTACAT GGTAATGGAG TACATGCCTG GTGGAGACCT TGTAAACCTT 
ATGAGTAATT ATGATGTGCC TGAAAAATGG GCCAAATTTT ACACTGCTGA AGTTGTTCTT 
GCTCTGGATG CAATACACTC CATGGGTTTA ATACACAGAG ATGTGAAGCC TGACAACATG 
CTCTTGGATA AACATGGACA TCTAAAATTA GCAGATTTTG GCACGTGTAT GAAGATGGAT 
GAAACAGGCA TGGTACATTG TGATACAGCA GTTGGAACAC CGGATTATAT ATCACCTGAG 
GTTCTGAAAT CACAAGGGGG TGATGGTTTC TATGGGCGAG AATGTGATTG GTGGTCTGTA 
GGTGTTTTCC TTTATGAGAT GCTAGTGGGG GATACTCCAT TTTATGCGGA TTCACTTGTA 
GGAACATATA GCAAAATTAT GGATCATAAG AATTCACTGT GTTTCCCTGA AGATGCAGAA 
ATTTCCAAAC ATGCAAAGAA TCTCATCTGT GCTTTCTTAA CAGATAGGGA GGTACGACTT 
GGGAGAAATG GGGTGGAAGA AATCAGACAG CATCCTTTCT TTAAGAATGA TCAGTGGCAT 
TGGGATAACA TAAGAGAAAC GGCAGCTCCT GTAGTACCTG AACTCAGCAG TGACATAGAC 
AGCAGCAATT TCGATGACAT TGAAGATGAC AAAGGAGATG TAGAAACCTT CCCAATTCCT 
AAAGCTTTTG TTGGAAATCA GCTGCCTTTC ATCGGATTTA CCTACTATAG AGAAAATTTA 
TTATTAAGTG ACTCTCCATC TTGTAGAGAA ACTGATTCCA TACAATCAAG GAAAAATGAA 
GAAAGTCAAG AGATTCAGAA AAAACTGTAT ACATTAGAAG AACATCTTAG CAATGAGATG 
CAAGCCAAAG AGGAACTGGA ACAGAAGTGC AAATCTGTTA ATACTCGCCT AGAAAAAACA 
GCAAAGGAGC TAGAAGAGGA GATTACCTTA CGGAAAAGTG TGGAATCAGC ATTAAGACAG 
TTAGAAAGAG AAAAGGCGCT TCTTCAGCAC AAAAATGCAG AATATCAGAG GAAAGCTGAT 
CATGAAGCAG ACAAAAAACG AAATTTGGAA AATGATGTTA ACAGCTTAAA AGATCAACTT 
GAAGATTTGA AAAAAAGAAA TCAAAACTCT CAAATATCCA CTGAGAAAGT GAATCAACTC 
CAGAGACAAC TGGATGAAAC CAATGCTTTA CTGCGAACAG AGTCTGATAC TGCAGCCCGG 
TTAAGGAAAA CCCAGGCAGA AAGTTCAAAA CAGATTCAGC AGCTGGAATC TAACAATAGA 
GATCTACAAG ATAAAAACTG CCTGCTGGAG ACTGCCAAGT TAAAACTTGA AAAGGAATTT 
ATCAATCTTC AGTCAGCTCT AGAATCTGAA AGGAGGGATC GAACCCATGG ATCAGAGATA 
ATTAATGATT TACAAGGTAG AATATGTGGC CTAGAAGAAG ATTTAAAGAA CGGCAAAATC 
TTACTAGCGA AAGTAGAACT GGAGAAGAGA CAACTTCAGG AGAGATTTAC TGATTTGGAA 
AAGGAAAAAA GCAACATGGA AATAGATATG ACATACCAAC TAAAAGTTAT ACAGCAGAGC 
CTAGAACAAG AAGAAGCTGA ACATAAGGCC ACAAAGGCAC GACTAGCAGA TAAAAATAAG 
ATCTATGAGT CCATCGAAGA AGCCAAATCA GAAGCCATGA AAGAAATGGA GAAGAAGCTC 
TTGGAGGAAA GAACTTTAAA ACAGAAAGTG GAGAACCTAT TGCTAGAAGC TGAGAAAAGA 
TGTTCTCTAT TAGACTGTGA CCTCAAACAG TCACAGCAGA AAATAAATGA GCTCCTTAAA 
CAGAAAGATG TGCTAAATGA GGATGTTAGA AACCTGACAT TAAAAATAGA GCAAGAAACT 
CAGAAGCGCT GCCTTACACA AAATGACCTG AAGATGCAAA CACAACAGGT TAACACACTA 
AAAATGTCAG AAAAGCAGTT AAAGCAAGAA AATAACCATC TCATGGAAAT GAAAATGAAC 
TTGGAAAAAC AAAATGCTGA ACTTCGAAAA GAACGTCAGG ATGCAGATGG GCAAATGAAA 
GAGCTCCAGG ATCAGCTCGA AGCAGAACAG TATTTCTCAA CCCTTTATAA AACACAAGTT 
AGGGAGCTTA AAGAAGAATG TGAAGAAAAG ACCAAACTTG GTAAAGAATT GCAGCAGAAG 
AAACAGGAAT TACAGGATGA ACGGGACTCT TTGGCTGCCC AACTGGAGAT CACCTTGACC 
AAAGCAGATT CTGAGCAACT GGCTCGTTCA ATTGCTGAAG AACAATATTC TGATTTGGAA 
AAAGAGAAGA TCATGAAAGA GCTGGAGATC AAAGAGATGA TGGCTAGACA CAAACAGGAA 
CTTACGGAAA AAGATGCTAC AATTGCTTCT CTTGAGGAAA CTAATAGGAC ACTAACTAGT 
GATGTTGCCA ATCTTGCAAA TGAGAAAGAA GAATTAAATA ACAAATTGAA AGATGTTCAA 
GAGCAACTGT CAAGATTGAA AGATGAAGAA ATAAGCGCAG CAGCTATTAA AGCACAGTTT 
GAGAAGCAGC TATTAACAGA AAGAACACTC AAAACTCAAG CTGTGAATAA GTTGGCTGAG 
ATCATGAATC GAAAAGAACC TGTCAAGCGT GGTAATGACA CAGATGTGCG GAGAAAAGAG 
AAGGAGAATA GAAAGCTACA TATGGAGCTT AAATCTGAAC GTGAGAAATT GACCCAGCAG 
ATGATCAAGT ATCAGAAAGA ACTGAATGAA ATGCAGGCAC AAATAGCTGA AGAGAGCCAG 
ATTCGAATTG AACTGCAGAT GACATTGGAC AGTAAAGACA GTGACATTGA GCAGCTGCGG 
TCACAACTCC AAGCCTTGCA TATTGGTCTG GATAGTTCCA GTATAGGCAG TGGACCAGGG 
GATGCTGAGG CAGATGATGG GTTTCCAGAA TCAAGATTAG AAGGATGGCT TTCATTGCCT 
GTACGAAACA ACACTAAGAA ATTTGGATGG GTTAAAAAGT ATGTGATTGT AAGCAGTAAG 
AAGATTCTTT TCTATGACAG TGAACAAGAT AAAGAACAAT CCAATCCTTA CATGGTTTTA 
GATATAGACA AGTTATTTCA TGTCCGACCA GTTACACAGA CAGATGTGTA TAGAGCAGAT 
GCTAAAGAAA TTCCAAGGAT ATTCCAGATT CTGTATGCCA ATGAAGGAGA AAGTAAGAAG 
GAACAAGAAT TTCCAGTGGA GCCAGTTGGA GAAAAATCTA ATTATATTTG CCACAAGGGA 
CATGAGTTTA TTCCTACTCT TTATCATTTC CCAACCAACT GTGAGGCTTG TATGAAGCCC 
CTGTGGCACA TGTTTAAGCC TCCTCCTGCT TTGGAGTGCC GCCGTTGCCA TATTAAGTGT 
CATAAAGATC ATATGGACAA AAAGGAGGAG ATTATAGCAC CTTGCAAAGT ATATTATGAT 
ATTTCAACGG CAAAGAATCT GTTATTACTA GCAAATTCTA CAGAAGAGCA GCAGAAGTGG 
GTTAGTCGGT TGGTGAAAAA GATACCTAAA AAGCCCCCAG CTCCAGACCC TTTTGCCCGA 
TCATCTCCTA GAACTTCAAT GAAGATACAG CAAAACCAGT CTATTAGACG GCCAAGTCGA 
CAGCTTGCCC CAAACAAACC TAGGGATGAA CAGAGCGCTT GGGGAAACGA GGGAAGCATG 
GAGAATTCTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860867.1
CDS	481..4695
Translation	MSRPPPTGKMPGAPETAPGDGAGASRQRKLEALIRDPRSPINVESLLDGLNSLVLDLDFPALRKNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGFYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDAEISKHAKNLICAFLTDREVRLGRNGVEEIRQHPFFKNDQWHWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYRENLLLSDSPSCRETDSIQSRKNEESQEIQKKLYTLEEHLSNEMQAKEELEQKCKSVNTRLEKTAKELEEEITLRKSVESALRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQNSQISTEKVNQLQRQLDETNALLRTESDTAARLRKTQAESSKQIQQLESNNRDLQDKNCLLETAKLKLEKEFINLQSALESERRDRTHGSEIINDLQGRICGLEEDLKNGKILLAKVELEKRQLQERFTDLEKEKSNMEIDMTYQLKVIQQSLEQEEAEHKATKARLADKNKIYESIEEAKSEAMKEMEKKLLEERTLKQKVENLLLEAEKRCSLLDCDLKQSQQKINELLKQKDVLNEDVRNLTLKIEQETQKRCLTQNDLKMQTQQVNTLKMSEKQLKQENNHLMEMKMNLEKQNAELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEECEEKTKLGKELQQKKQELQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELTEKDATIASLEETNRTLTSDVANLANEKEELNNKLKDVQEQLSRLKDEEISAAAIKAQFEKQLLTERTLKTQAVNKLAEIMNRKEPVKRGNDTDVRRKEKENRKLHMELKSEREKLTQQMIKYQKELNEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQALHIGLDSSSIGSGPGDAEADDGFPESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEQEFPVEPVGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISTAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPRDEQSAWGNEGSMENSA

Target ORF information:

RefSeq Version	XM_017005378.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005378.1

ORF Insert Sequence:

ATGAGCCGGC CCCCGCCGAC GGGGAAAATG CCCGGCGCCC CCGAGACCGC GCCGGGGGAC 
GGGGCAGGCG CGAGCCGCCA GAGGAAGCTG GAGGCGCTGA TCCGAGACCC TCGCTCCCCC 
ATCAACGTGG AGAGCTTGCT GGATGGCTTA AATTCCTTGG TCCTTGATTT AGATTTTCCT 
GCTTTGAGGA AAAACAAGAA CATAGATAAT TTCTTAAATA GATATGAGAA AATTGTGAAA 
AAAATCAGAG GTCTACAGAT GAAGGCAGAA GACTATGATG TTGTAAAAGT TATTGGAAGA 
GGTGCTTTTG GTGAAGTGCA GTTGGTTCGT CACAAGGCAT CGCAGAAGGT TTATGCTATG 
AAGCTTCTTA GTAAGTTTGA AATGATAAAA AGATCAGATT CTGCCTTTTT TTGGGAAGAA 
AGAGATATTA TGGCCTTTGC CAATAGCCCC TGGGTGGTTC AGCTTTTTTA TGCCTTTCAA 
GATGATAGGT ATCTGTACAT GGTAATGGAG TACATGCCTG GTGGAGACCT TGTAAACCTT 
ATGAGTAATT ATGATGTGCC TGAAAAATGG GCCAAATTTT ACACTGCTGA AGTTGTTCTT 
GCTCTGGATG CAATACACTC CATGGGTTTA ATACACAGAG ATGTGAAGCC TGACAACATG 
CTCTTGGATA AACATGGACA TCTAAAATTA GCAGATTTTG GCACGTGTAT GAAGATGGAT 
GAAACAGGCA TGGTACATTG TGATACAGCA GTTGGAACAC CGGATTATAT ATCACCTGAG 
GTTCTGAAAT CACAAGGGGG TGATGGTTTC TATGGGCGAG AATGTGATTG GTGGTCTGTA 
GGTGTTTTCC TTTATGAGAT GCTAGTGGGG GATACTCCAT TTTATGCGGA TTCACTTGTA 
GGAACATATA GCAAAATTAT GGATCATAAG AATTCACTGT GTTTCCCTGA AGATGCAGAA 
ATTTCCAAAC ATGCAAAGAA TCTCATCTGT GCTTTCTTAA CAGATAGGGA GGTACGACTT 
GGGAGAAATG GGGTGGAAGA AATCAGACAG CATCCTTTCT TTAAGAATGA TCAGTGGCAT 
TGGGATAACA TAAGAGAAAC GGCAGCTCCT GTAGTACCTG AACTCAGCAG TGACATAGAC 
AGCAGCAATT TCGATGACAT TGAAGATGAC AAAGGAGATG TAGAAACCTT CCCAATTCCT 
AAAGCTTTTG TTGGAAATCA GCTGCCTTTC ATCGGATTTA CCTACTATAG AGAAAATTTA 
TTATTAAGTG ACTCTCCATC TTGTAGAGAA ACTGATTCCA TACAATCAAG GAAAAATGAA 
GAAAGTCAAG AGATTCAGAA AAAACTGTAT ACATTAGAAG AACATCTTAG CAATGAGATG 
CAAGCCAAAG AGGAACTGGA ACAGAAGTGC AAATCTGTTA ATACTCGCCT AGAAAAAACA 
GCAAAGGAGC TAGAAGAGGA GATTACCTTA CGGAAAAGTG TGGAATCAGC ATTAAGACAG 
TTAGAAAGAG AAAAGGCGCT TCTTCAGCAC AAAAATGCAG AATATCAGAG GAAAGCTGAT 
CATGAAGCAG ACAAAAAACG AAATTTGGAA AATGATGTTA ACAGCTTAAA AGATCAACTT 
GAAGATTTGA AAAAAAGAAA TCAAAACTCT CAAATATCCA CTGAGAAAGT GAATCAACTC 
CAGAGACAAC TGGATGAAAC CAATGCTTTA CTGCGAACAG AGTCTGATAC TGCAGCCCGG 
TTAAGGAAAA CCCAGGCAGA AAGTTCAAAA CAGATTCAGC AGCTGGAATC TAACAATAGA 
GATCTACAAG ATAAAAACTG CCTGCTGGAG ACTGCCAAGT TAAAACTTGA AAAGGAATTT 
ATCAATCTTC AGTCAGCTCT AGAATCTGAA AGGAGGGATC GAACCCATGG ATCAGAGATA 
ATTAATGATT TACAAGGTAG AATATGTGGC CTAGAAGAAG ATTTAAAGAA CGGCAAAATC 
TTACTAGCGA AAGTAGAACT GGAGAAGAGA CAACTTCAGG AGAGATTTAC TGATTTGGAA 
AAGGAAAAAA GCAACATGGA AATAGATATG ACATACCAAC TAAAAGTTAT ACAGCAGAGC 
CTAGAACAAG AAGAAGCTGA ACATAAGGCC ACAAAGGCAC GACTAGCAGA TAAAAATAAG 
ATCTATGAGT CCATCGAAGA AGCCAAATCA GAAGCCATGA AAGAAATGGA GAAGAAGCTC 
TTGGAGGAAA GAACTTTAAA ACAGAAAGTG GAGAACCTAT TGCTAGAAGC TGAGAAAAGA 
TGTTCTCTAT TAGACTGTGA CCTCAAACAG TCACAGCAGA AAATAAATGA GCTCCTTAAA 
CAGAAAGATG TGCTAAATGA GGATGTTAGA AACCTGACAT TAAAAATAGA GCAAGAAACT 
CAGAAGCGCT GCCTTACACA AAATGACCTG AAGATGCAAA CACAACAGGT TAACACACTA 
AAAATGTCAG AAAAGCAGTT AAAGCAAGAA AATAACCATC TCATGGAAAT GAAAATGAAC 
TTGGAAAAAC AAAATGCTGA ACTTCGAAAA GAACGTCAGG ATGCAGATGG GCAAATGAAA 
GAGCTCCAGG ATCAGCTCGA AGCAGAACAG TATTTCTCAA CCCTTTATAA AACACAAGTT 
AGGGAGCTTA AAGAAGAATG TGAAGAAAAG ACCAAACTTG GTAAAGAATT GCAGCAGAAG 
AAACAGGAAT TACAGGATGA ACGGGACTCT TTGGCTGCCC AACTGGAGAT CACCTTGACC 
AAAGCAGATT CTGAGCAACT GGCTCGTTCA ATTGCTGAAG AACAATATTC TGATTTGGAA 
AAAGAGAAGA TCATGAAAGA GCTGGAGATC AAAGAGATGA TGGCTAGACA CAAACAGGAA 
CTTACGGAAA AAGATGCTAC AATTGCTTCT CTTGAGGAAA CTAATAGGAC ACTAACTAGT 
GATGTTGCCA ATCTTGCAAA TGAGAAAGAA GAATTAAATA ACAAATTGAA AGATGTTCAA 
GAGCAACTGT CAAGATTGAA AGATGAAGAA ATAAGCGCAG CAGCTATTAA AGCACAGTTT 
GAGAAGCAGC TATTAACAGA AAGAACACTC AAAACTCAAG CTGTGAATAA GTTGGCTGAG 
ATCATGAATC GAAAAGAACC TGTCAAGCGT GGTAATGACA CAGATGTGCG GAGAAAAGAG 
AAGGAGAATA GAAAGCTACA TATGGAGCTT AAATCTGAAC GTGAGAAATT GACCCAGCAG 
ATGATCAAGT ATCAGAAAGA ACTGAATGAA ATGCAGGCAC AAATAGCTGA AGAGAGCCAG 
ATTCGAATTG AACTGCAGAT GACATTGGAC AGTAAAGACA GTGACATTGA GCAGCTGCGG 
TCACAACTCC AAGCCTTGCA TATTGGTCTG GATAGTTCCA GTATAGGCAG TGGACCAGGG 
GATGCTGAGG CAGATGATGG GTTTCCAGAA TCAAGATTAG AAGGATGGCT TTCATTGCCT 
GTACGAAACA ACACTAAGAA ATTTGGATGG GTTAAAAAGT ATGTGATTGT AAGCAGTAAG 
AAGATTCTTT TCTATGACAG TGAACAAGAT AAAGAACAAT CCAATCCTTA CATGGTTTTA 
GATATAGACA AGTTATTTCA TGTCCGACCA GTTACACAGA CAGATGTGTA TAGAGCAGAT 
GCTAAAGAAA TTCCAAGGAT ATTCCAGATT CTGTATGCCA ATGAAGGAGA AAGTAAGAAG 
GAACAAGAAT TTCCAGTGGA GCCAGTTGGA GAAAAATCTA ATTATATTTG CCACAAGGGA 
CATGAGTTTA TTCCTACTCT TTATCATTTC CCAACCAACT GTGAGGCTTG TATGAAGCCC 
CTGTGGCACA TGTTTAAGCC TCCTCCTGCT TTGGAGTGCC GCCGTTGCCA TATTAAGTGT 
CATAAAGATC ATATGGACAA AAAGGAGGAG ATTATAGCAC CTTGCAAAGT ATATTATGAT 
ATTTCAACGG CAAAGAATCT GTTATTACTA GCAAATTCTA CAGAAGAGCA GCAGAAGTGG 
GTTAGTCGGT TGGTGAAAAA GATACCTAAA AAGCCCCCAG CTCCAGACCC TTTTGCCCGA 
TCATCTCCTA GAACTTCAAT GAAGATACAG CAAAACCAGT CTATTAGACG GCCAAGTCGA 
CAGCTTGCCC CAAACAAACC TAGGGATGAA CAGAGCGCTT GGGGAAACGA GGGAAGCATG 
GAGAATTCTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ROCK2
Entrez Gene ID	9475
Full Name	Rho associated coiled-coil containing protein kinase 2
Synonyms	ROCK-II
General protein information	Preferred Names Rho associated coiled-coil containing protein kinase 2 Names rho-associated protein kinase 2 p164 ROCK-2 rho-associated, coiled-coil-containing protein kinase II
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p25.1
Summary	The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq, Jul 2008].
Disorder MIM:	604002

Mus musculus (house mouse)	Rock2	NP_033098.2
Drosophila melanogaster (fruit fly)	rok	NP_536796.2
Caenorhabditis elegans (roundworm)	let-502	NP_491440.1
Pan troglodytes (chimpanzee)	ROCK2	XP_525689.3
Rattus norvegicus (Norway rat)	Rock2	NP_037154.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000406	XP_310694.5
Xenopus tropicalis (tropical clawed frog)	rock2	XP_004914525.1
Homo sapiens (human)	ROCK2	NP_004841.2
	ROCK2	XP_001096931.2
Canis lupus familiaris (dog)	ROCK2	XP_540083.4
Danio rerio (zebrafish)	rock2a	NP_777288.1
Bos taurus (cattle)	ROCK2	NP_776877.1
Gallus gallus (chicken)	ROCK2	XP_004935820.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ROCK2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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