LOC100215556 cDNA ORF clone, Hydra vulgaris

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100215556
Entrez Gene ID	100215556
Full Name	alpha-actinin, sarcomeric-like
Gene Type	protein-coding
Organism	Hydra vulgaris

mRNA and Protein(s)

mRNA	Protein	Name
XM_004208528.2	XP_004208576.1	alpha-actinin, sarcomeric-like

Pathways from BioSystems
Gene Ontology

The following LOC100215556 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100215556 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHc06995	XM_004208528.2 Latest version!	Hydra vulgaris alpha-actinin, sarcomeric-like (LOC100215556), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100215556 ( XM_004208528.2 ) cDNA ORF clone, Hydra vulgaris -> XP_004208576.1 Hydra vulgaris alpha-actinin, sarcomeric-like (LOC100215556), mRNA.

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CloneID	OHc06995
Clone ID Related Accession (Same CDS sequence)	XM_004208528.2
Accession Version	XM_004208528.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2640bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-07
Organism	Hydra vulgaris
Product	alpha-actinin, sarcomeric-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004168652.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 8, 2015 this sequence version replaced XM_004208528.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Hydra vulgaris Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAAG AAGAACATTA TGGATACCAT CAGGAAGAAG AAGACAGAGG GTTGCTTGAT 
CCAGCATGGG AAATGCAACA AAAAAAGACA TTTACAGCAT GGTGCAATTC ACACTTAAGG 
AAAGTTAATG TTCAAATAGA AGAAATATCA AGAGACTTTA GTGATGGACT TAAACTTATG 
TTGCTTCTTG AAGTTATTGC AGGAGAAAGA CTACCAAAAC CTGAAAGAGG TCGTTTACGG 
TTTCATCAAA TATCTAATGT TAACAAAGCC TTAGACTTTG TTGCTAGCAA AGGAGTAAAA 
TTGGTTTCCA TTGGTGCTGA AGAAATTGTT GATGGTAACT TAAAAATGAT TTTGGGTATG 
ATTTGGACAA TCATTTTGAG ATTCGCCATT CAAGATATTC TTGTTGATGA GTTTTCATCT 
AAGGAAGGAT TACTGCTTTG GTGTCAAAGA AAAACTGCTC CTTACAAAAA TGTTAATGTT 
CAAAATTTTC ACATGAGTTT TAAAGATGGT CTTGCCTTCT GTGCTCTTAT TCACCGTCAC 
CGACCTGATC TCATTGATTA CAACAAGTTG TCTAAGGATG ATCCAATGCA CAATTTAAAT 
TATGCTTTTG ATGTTGCTGA TAAATACCTT GATATCCCTC GTATGTTGGA TGCTGAAGAC 
ATGGTTAACC AAGTTAAACC AGATGAAAGA GCTGTTATGA CTTATGTATC TTGCTACTAT 
CATGCTTTTT CAAGTTCTCA ACAGGCTGAA ACAGCTGCAA AACGCATTGT TAAAGTATTA 
AATGTTAATC AAGGTAATGA AAAAGCAATG GATGATTATG AAAATATGGC ATCTCAGTTA 
CTTGATTGGA TTAATAAAAA AATTCCTGAG CTTGAAGACC GAACTACTGA TAATACTCTT 
AAAGATGTCC AGAATAAGCT GAATGACTTC AGAAATTACA GAGGCACTGA GAAACCACCA 
AAGGTTGAAG AGAAAGCAGG TTTAGAAAAT TTGTTTAACA CGTTACAGAC TAAGTTACGA 
TTAAGCAATC GCCCAGTTTA TATGCCAACT GAAGGCAAAA TGTTATCTGA CATAGAAAAA 
TCTTGGAAAG ATTTGGAACA GGCTGAAAAA GATTTCCAAG ATTGGATTCT TGCAGAACTT 
CGGAAGTTGG AGCGACTAGA TCATCTAGCT GCAAAATTTA AGCACAAGTG TGATATTCAT 
GAAGCTTGGT CCCAAGGTCA AGATGTATAT TTGACAACTG ATGACATTAC TGGTGCTCCT 
CTTGCATCTC TATTGGCTTC TCGCAAACGT CATGAAGCAT TTCAAGCTGA TATATCTGCT 
CAACAAGATC GTGTGGAACA GATCGTAGCA ATTGCTCAAG AATTATACAC ACTTGGCTAC 
AGCAAAATTA ATGAGGTTAA TGAACGCTGT AGGATTATAT GTGAAGAATG GGATAACCTT 
GGAGAATTAA ATGAAAAACG TAACAAAAAT TTGACAGCTG CTATTGAAAT TGCTGAGTAT 
CTTGATAATC TCTGGCTTGA ATATGCAAAG AAGGCATCGC CATTTAGTAA CTGGATGGAT 
GGTGCTAAAG AAGATTTAAT GGATATGTTT AATGTCCACT CTTTAGAAGA AATGCAAGAT 
CTCAAAGATG CACAAGAAAA ATTTAAAGCT AATATGCCAG CTGCAAAAGA AGAATTTGAG 
TTAATAATGA GTCTGGTTCG TGAAATTAAC TCAATGAGTA ATCAAAAAAA TCCATATACT 
AACACAAGAT CTGAGGATTT AGAACAAAAG TGGAATGACG TTATTGAATT GATACCTAAA 
AGAGATAGTA AGTTATCTGC TGAAACAATT CGCCAACAGA CTAATGAAAG ACTTCGTGTG 
CAGTTTGCAC AGATGGCAAA TCAAGTTGGT GAATGGATCA AACAAAGAAC AAGTAGAATG 
AGAGATATTG GAATGAATGC AAGAGGAAGT CTAGAAAGTC AGCTTAAAGA GCTAAACGCT 
CTTAATGATG AAATCAATGG CTTTAAAGAA CATATTTTGG AGATTGATGC TTTGAATGCT 
TCAGTACAGG CTGCTATGAT CTTTGAGAAT CCTCACACAT CATTAACAAT GGAAATGATT 
CGCATTCGAT TTGAACTTCT TCTTACTGGT ATGACAAGAA ACATTAATGA GGTTGAAAAT 
CAGATTTTGA TGAGAGATTC TATTGGCATA AGTGAGGACC AATTGAAAGA ATTGAAAAGT 
TCATTCAGTT TCTTTGACAA AAATAAAAAT AACCAGTTAG ATTATAAGGA ATTTAGACAG 
TGCTTGGTCA GTCTTGGTCA CAGTGTCGAT GTTGAAGATA AGGGTGATGC TGAGTTTGCA 
CGTATTATGG CTATGGTTGA TCCCAATCAA ACAGGATTTG TTACATTCCA AGCTTTCCTT 
GATTTTATGA CTAGGGAAAT GGCAGATACT GACAGTGCTG AACAAGTTAT GGAGTCATTT 
AGAATTTTAG CGGGTGATAA ACCTTACATT ACTGCAGATG AACTACGCCG TGAGTTGCCT 
CCCCAACAAG CTGATTATTG TATTGCTCGA ATGGCTGCAT ATACTGGCCC GGGATCAGTC 
CCAGGCGCTC TTGATTATAA ATCTTTTTCT ACTGCCTTAT ATGGTGAAAG CGATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004208576.1
CDS	164..2803
Translation	MAEEEHYGYHQEEEDRGLLDPAWEMQQKKTFTAWCNSHLRKVNVQIEEISRDFSDGLKLMLLLEVIAGERLPKPERGRLRFHQISNVNKALDFVASKGVKLVSIGAEEIVDGNLKMILGMIWTIILRFAIQDILVDEFSSKEGLLLWCQRKTAPYKNVNVQNFHMSFKDGLAFCALIHRHRPDLIDYNKLSKDDPMHNLNYAFDVADKYLDIPRMLDAEDMVNQVKPDERAVMTYVSCYYHAFSSSQQAETAAKRIVKVLNVNQGNEKAMDDYENMASQLLDWINKKIPELEDRTTDNTLKDVQNKLNDFRNYRGTEKPPKVEEKAGLENLFNTLQTKLRLSNRPVYMPTEGKMLSDIEKSWKDLEQAEKDFQDWILAELRKLERLDHLAAKFKHKCDIHEAWSQGQDVYLTTDDITGAPLASLLASRKRHEAFQADISAQQDRVEQIVAIAQELYTLGYSKINEVNERCRIICEEWDNLGELNEKRNKNLTAAIEIAEYLDNLWLEYAKKASPFSNWMDGAKEDLMDMFNVHSLEEMQDLKDAQEKFKANMPAAKEEFELIMSLVREINSMSNQKNPYTNTRSEDLEQKWNDVIELIPKRDSKLSAETIRQQTNERLRVQFAQMANQVGEWIKQRTSRMRDIGMNARGSLESQLKELNALNDEINGFKEHILEIDALNASVQAAMIFENPHTSLTMEMIRIRFELLLTGMTRNINEVENQILMRDSIGISEDQLKELKSSFSFFDKNKNNQLDYKEFRQCLVSLGHSVDVEDKGDAEFARIMAMVDPNQTGFVTFQAFLDFMTREMADTDSAEQVMESFRILAGDKPYITADELRRELPPQQADYCIARMAAYTGPGSVPGALDYKSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_004208528.2
Organism	Hydra vulgaris
Definition	Hydra vulgaris alpha-actinin, sarcomeric-like (LOC100215556), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004208528.2

ORF Insert Sequence:

ATGGCTGAAG AAGAACATTA TGGATACCAT CAGGAAGAAG AAGACAGAGG GTTGCTTGAT 
CCAGCATGGG AAATGCAACA AAAAAAGACA TTTACAGCAT GGTGCAATTC ACACTTAAGG 
AAAGTTAATG TTCAAATAGA AGAAATATCA AGAGACTTTA GTGATGGACT TAAACTTATG 
TTGCTTCTTG AAGTTATTGC AGGAGAAAGA CTACCAAAAC CTGAAAGAGG TCGTTTACGG 
TTTCATCAAA TATCTAATGT TAACAAAGCC TTAGACTTTG TTGCTAGCAA AGGAGTAAAA 
TTGGTTTCCA TTGGTGCTGA AGAAATTGTT GATGGTAACT TAAAAATGAT TTTGGGTATG 
ATTTGGACAA TCATTTTGAG ATTCGCCATT CAAGATATTC TTGTTGATGA GTTTTCATCT 
AAGGAAGGAT TACTGCTTTG GTGTCAAAGA AAAACTGCTC CTTACAAAAA TGTTAATGTT 
CAAAATTTTC ACATGAGTTT TAAAGATGGT CTTGCCTTCT GTGCTCTTAT TCACCGTCAC 
CGACCTGATC TCATTGATTA CAACAAGTTG TCTAAGGATG ATCCAATGCA CAATTTAAAT 
TATGCTTTTG ATGTTGCTGA TAAATACCTT GATATCCCTC GTATGTTGGA TGCTGAAGAC 
ATGGTTAACC AAGTTAAACC AGATGAAAGA GCTGTTATGA CTTATGTATC TTGCTACTAT 
CATGCTTTTT CAAGTTCTCA ACAGGCTGAA ACAGCTGCAA AACGCATTGT TAAAGTATTA 
AATGTTAATC AAGGTAATGA AAAAGCAATG GATGATTATG AAAATATGGC ATCTCAGTTA 
CTTGATTGGA TTAATAAAAA AATTCCTGAG CTTGAAGACC GAACTACTGA TAATACTCTT 
AAAGATGTCC AGAATAAGCT GAATGACTTC AGAAATTACA GAGGCACTGA GAAACCACCA 
AAGGTTGAAG AGAAAGCAGG TTTAGAAAAT TTGTTTAACA CGTTACAGAC TAAGTTACGA 
TTAAGCAATC GCCCAGTTTA TATGCCAACT GAAGGCAAAA TGTTATCTGA CATAGAAAAA 
TCTTGGAAAG ATTTGGAACA GGCTGAAAAA GATTTCCAAG ATTGGATTCT TGCAGAACTT 
CGGAAGTTGG AGCGACTAGA TCATCTAGCT GCAAAATTTA AGCACAAGTG TGATATTCAT 
GAAGCTTGGT CCCAAGGTCA AGATGTATAT TTGACAACTG ATGACATTAC TGGTGCTCCT 
CTTGCATCTC TATTGGCTTC TCGCAAACGT CATGAAGCAT TTCAAGCTGA TATATCTGCT 
CAACAAGATC GTGTGGAACA GATCGTAGCA ATTGCTCAAG AATTATACAC ACTTGGCTAC 
AGCAAAATTA ATGAGGTTAA TGAACGCTGT AGGATTATAT GTGAAGAATG GGATAACCTT 
GGAGAATTAA ATGAAAAACG TAACAAAAAT TTGACAGCTG CTATTGAAAT TGCTGAGTAT 
CTTGATAATC TCTGGCTTGA ATATGCAAAG AAGGCATCGC CATTTAGTAA CTGGATGGAT 
GGTGCTAAAG AAGATTTAAT GGATATGTTT AATGTCCACT CTTTAGAAGA AATGCAAGAT 
CTCAAAGATG CACAAGAAAA ATTTAAAGCT AATATGCCAG CTGCAAAAGA AGAATTTGAG 
TTAATAATGA GTCTGGTTCG TGAAATTAAC TCAATGAGTA ATCAAAAAAA TCCATATACT 
AACACAAGAT CTGAGGATTT AGAACAAAAG TGGAATGACG TTATTGAATT GATACCTAAA 
AGAGATAGTA AGTTATCTGC TGAAACAATT CGCCAACAGA CTAATGAAAG ACTTCGTGTG 
CAGTTTGCAC AGATGGCAAA TCAAGTTGGT GAATGGATCA AACAAAGAAC AAGTAGAATG 
AGAGATATTG GAATGAATGC AAGAGGAAGT CTAGAAAGTC AGCTTAAAGA GCTAAACGCT 
CTTAATGATG AAATCAATGG CTTTAAAGAA CATATTTTGG AGATTGATGC TTTGAATGCT 
TCAGTACAGG CTGCTATGAT CTTTGAGAAT CCTCACACAT CATTAACAAT GGAAATGATT 
CGCATTCGAT TTGAACTTCT TCTTACTGGT ATGACAAGAA ACATTAATGA GGTTGAAAAT 
CAGATTTTGA TGAGAGATTC TATTGGCATA AGTGAGGACC AATTGAAAGA ATTGAAAAGT 
TCATTCAGTT TCTTTGACAA AAATAAAAAT AACCAGTTAG ATTATAAGGA ATTTAGACAG 
TGCTTGGTCA GTCTTGGTCA CAGTGTCGAT GTTGAAGATA AGGGTGATGC TGAGTTTGCA 
CGTATTATGG CTATGGTTGA TCCCAATCAA ACAGGATTTG TTACATTCCA AGCTTTCCTT 
GATTTTATGA CTAGGGAAAT GGCAGATACT GACAGTGCTG AACAAGTTAT GGAGTCATTT 
AGAATTTTAG CGGGTGATAA ACCTTACATT ACTGCAGATG AACTACGCCG TGAGTTGCCT 
CCCCAACAAG CTGATTATTG TATTGCTCGA ATGGCTGCAT ATACTGGCCC GGGATCAGTC 
CCAGGCGCTC TTGATTATAA ATCTTTTTCT ACTGCCTTAT ATGGTGAAAG CGATTTATAG