LOC100877003 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100877003
Entrez Gene ID	100877003
Full Name	1,4-alpha-glucan-branching enzyme
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003707197.2	XP_003707245.1	1,4-alpha-glucan-branching enzyme

Pathways from BioSystems
Gene Ontology

The following LOC100877003 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100877003 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe112661	XM_003707197.2 Latest version!	Megachile rotundata 1,4-alpha-glucan-branching enzyme (LOC100877003), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100877003 ( XM_003707197.2 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_003707245.1 Megachile rotundata 1,4-alpha-glucan-branching enzyme (LOC100877003), mRNA.

Price & Availability↑

CloneID	OMe112661
Clone ID Related Accession (Same CDS sequence)	XM_003707197.2
Accession Version	XM_003707197.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	1,4-alpha-glucan-branching enzyme
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797515.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2015 this sequence version replaced XM_003707197.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGGAA AATGGTCGAG CATGGATCCT TCCCAAGTGG AAGTGCCAGA AATAGGTGCT 
TTATTAGAAA GAGATCCTTA TTTAAAACCT TATGAAAATG AAATTCGTAA AAGATATGCA 
TTATTCAAAG ATTACATGGA AAAATTAGAA ACTGGTGATG GCAGCTTAGA AAAATTTTCA 
AAAGCATATA AAAACTTTGG AATTCATATA AATGAAGACA ATAGCGTTAC TGCAAAAGAA 
TGGGCACCTG GTGCACAAGA ATTATTTTTA ACAGGAGATT TTAATAACTG GAATAAAACA 
GCTAACTCAT ATAAAAAGTT AGAATATGGA AAATGGGAAT TATATTTACC TCCTAATGCC 
GATGGTAGTT GTCCTATAAA ACATCTTTCA GAAGTTAAAA TAATTGTAAA GGATCACAAC 
AATGAATTGT TGGAACGATT AAGTCCTTGG GCTAATTATG TTACACAAAA TCGTGCTGAA 
AGTGCTACGT ATAAACAACG TATATGGCAT CCATTATCTA AAAATACTTA TAGATTTAAA 
TACCCCAAGC CAAAAAAACC AGAAAGTCTT AGAATTTATG AATGCCATGT TGGTATTGCA 
ACTCAAGAAT TAAAAGTTGG TACATATTTA GAATTTGCTA AAAATGTAAT TCCTCGCATT 
GTAAAACAGG GTTATAATGC AATACAACTA ATGGCTATTA TGGAACATGC TTATTATGCC 
AGTTTTGGAT ATCAGGTGAC TTCCTTTTAT GCAGCTTCAT CGCGTTATGG TACACCAGAG 
GAATTAAAAG AGTTAATAGA TGTAGCACAT GAACATGGAC TATTTGTTTT ACTAGATATG 
GTGCATTCAC ATGCATCAAA GAATACTTTG GATGGGTTAA ATATGTTTGA TGGTACTGAT 
GGTTGTTTCT TTCATGCTGG TAATCGTGGT CAGCATCCGC TTTGGGATAG TCGGCTCTTT 
AATTATGGAG AATATGAAGT ATTGCGATTT TTACTTTCTA ATTTACGATG GTACATCGAT 
GAGTATGGTT TTGATGGATT TAGATTTGAT GGCGTTACAT CAATGTTATA TCACTCGAGA 
GGATTTGGAC AAGGTTTTAG TGGCCATTAC GATGAATATT ATGGCCTAAA TGTTGATGTT 
GAGGGTGTAG TATATTTGAT GCTTGCAAAT CACATGTTGC ATCATTTGTA TCCAGAAATT 
GTTACAATAG CAGAAGATGT TAGTGGAATG CCTGGAGTAT GCAGACCTGT CTCTGAAGGT 
GGTGTAGGAT TTGATTACCG ATTGGGTATG TCTATTCCTG ACAAATGGAT TAAGTTGTTG 
AAAGAAGTAA AAGATGAAGA TTGGAAAGTT GGAGACATTT GTTGGTCGTT AAGTAATCGA 
AGGTGGATGG AGAAAACGGT AGCTTACGCG GAATCTCATG ATCAAGCTCT CGTGGGTGAT 
AAAACAATTG CATTCTGGCT TATGGACAAA GAAATGTATA CTCATATGAG TATAATGAGT 
CCGCCAAATC CGATCATAAG TCGTGGAATT GCTCTTCATA ATCTTATTAC ATTAATTACA 
CATGCATTAG GAGGAGAAGC GTATTTAAAT TTTATGGGTA ATGAATTCGG TCATCCTGAA 
TGGTTGGATT TTCCTCGAGC TGGTAACGGG GATAGCTATC ATTATGCTAG AAGGCAATGG 
AATTTAGTTG ACGATGAATT GCTGAAATAT AAGTTTATGA ATAATTGGGA CCGCGCTATA 
AATACTCTTG AAGAAAAATA TGGATGGCTA CATGCTCATC CTGCATATAT AAGTTGGAAA 
CATGAAGGAG ACAAAGTAAT TGTTTTTGAT CGGGCAGAAC TTATATTTGT TTTTAATTTT 
CATCCGGTAC AGTCATTTCC GGATTATCCT ATTGGCGTAA AGACTGCAGG AACTTACAAA 
GTTCTTTTGT GTAGCGACAA TAAGGATTTT GGTGGCGAAA ATCGTGTTGA TACCAGTATA 
CAACATTTTA CCCAACCAGA ATCATTTTCT GATTATTCAA ATAAAATGTT GATTTATATT 
CCTTGTCGCA CAGCTATTGT CTACGTCCGA GAAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003707245.1
CDS	87..2165
Translation	MGGKWSSMDPSQVEVPEIGALLERDPYLKPYENEIRKRYALFKDYMEKLETGDGSLEKFSKAYKNFGIHINEDNSVTAKEWAPGAQELFLTGDFNNWNKTANSYKKLEYGKWELYLPPNADGSCPIKHLSEVKIIVKDHNNELLERLSPWANYVTQNRAESATYKQRIWHPLSKNTYRFKYPKPKKPESLRIYECHVGIATQELKVGTYLEFAKNVIPRIVKQGYNAIQLMAIMEHAYYASFGYQVTSFYAASSRYGTPEELKELIDVAHEHGLFVLLDMVHSHASKNTLDGLNMFDGTDGCFFHAGNRGQHPLWDSRLFNYGEYEVLRFLLSNLRWYIDEYGFDGFRFDGVTSMLYHSRGFGQGFSGHYDEYYGLNVDVEGVVYLMLANHMLHHLYPEIVTIAEDVSGMPGVCRPVSEGGVGFDYRLGMSIPDKWIKLLKEVKDEDWKVGDICWSLSNRRWMEKTVAYAESHDQALVGDKTIAFWLMDKEMYTHMSIMSPPNPIISRGIALHNLITLITHALGGEAYLNFMGNEFGHPEWLDFPRAGNGDSYHYARRQWNLVDDELLKYKFMNNWDRAINTLEEKYGWLHAHPAYISWKHEGDKVIVFDRAELIFVFNFHPVQSFPDYPIGVKTAGTYKVLLCSDNKDFGGENRVDTSIQHFTQPESFSDYSNKMLIYIPCRTAIVYVRET

Target ORF information:

RefSeq Version	XM_003707197.2
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata 1,4-alpha-glucan-branching enzyme (LOC100877003), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003707197.2

ORF Insert Sequence:

ATGGGAGGAA AATGGTCGAG CATGGATCCT TCCCAAGTGG AAGTGCCAGA AATAGGTGCT 
TTATTAGAAA GAGATCCTTA TTTAAAACCT TATGAAAATG AAATTCGTAA AAGATATGCA 
TTATTCAAAG ATTACATGGA AAAATTAGAA ACTGGTGATG GCAGCTTAGA AAAATTTTCA 
AAAGCATATA AAAACTTTGG AATTCATATA AATGAAGACA ATAGCGTTAC TGCAAAAGAA 
TGGGCACCTG GTGCACAAGA ATTATTTTTA ACAGGAGATT TTAATAACTG GAATAAAACA 
GCTAACTCAT ATAAAAAGTT AGAATATGGA AAATGGGAAT TATATTTACC TCCTAATGCC 
GATGGTAGTT GTCCTATAAA ACATCTTTCA GAAGTTAAAA TAATTGTAAA GGATCACAAC 
AATGAATTGT TGGAACGATT AAGTCCTTGG GCTAATTATG TTACACAAAA TCGTGCTGAA 
AGTGCTACGT ATAAACAACG TATATGGCAT CCATTATCTA AAAATACTTA TAGATTTAAA 
TACCCCAAGC CAAAAAAACC AGAAAGTCTT AGAATTTATG AATGCCATGT TGGTATTGCA 
ACTCAAGAAT TAAAAGTTGG TACATATTTA GAATTTGCTA AAAATGTAAT TCCTCGCATT 
GTAAAACAGG GTTATAATGC AATACAACTA ATGGCTATTA TGGAACATGC TTATTATGCC 
AGTTTTGGAT ATCAGGTGAC TTCCTTTTAT GCAGCTTCAT CGCGTTATGG TACACCAGAG 
GAATTAAAAG AGTTAATAGA TGTAGCACAT GAACATGGAC TATTTGTTTT ACTAGATATG 
GTGCATTCAC ATGCATCAAA GAATACTTTG GATGGGTTAA ATATGTTTGA TGGTACTGAT 
GGTTGTTTCT TTCATGCTGG TAATCGTGGT CAGCATCCGC TTTGGGATAG TCGGCTCTTT 
AATTATGGAG AATATGAAGT ATTGCGATTT TTACTTTCTA ATTTACGATG GTACATCGAT 
GAGTATGGTT TTGATGGATT TAGATTTGAT GGCGTTACAT CAATGTTATA TCACTCGAGA 
GGATTTGGAC AAGGTTTTAG TGGCCATTAC GATGAATATT ATGGCCTAAA TGTTGATGTT 
GAGGGTGTAG TATATTTGAT GCTTGCAAAT CACATGTTGC ATCATTTGTA TCCAGAAATT 
GTTACAATAG CAGAAGATGT TAGTGGAATG CCTGGAGTAT GCAGACCTGT CTCTGAAGGT 
GGTGTAGGAT TTGATTACCG ATTGGGTATG TCTATTCCTG ACAAATGGAT TAAGTTGTTG 
AAAGAAGTAA AAGATGAAGA TTGGAAAGTT GGAGACATTT GTTGGTCGTT AAGTAATCGA 
AGGTGGATGG AGAAAACGGT AGCTTACGCG GAATCTCATG ATCAAGCTCT CGTGGGTGAT 
AAAACAATTG CATTCTGGCT TATGGACAAA GAAATGTATA CTCATATGAG TATAATGAGT 
CCGCCAAATC CGATCATAAG TCGTGGAATT GCTCTTCATA ATCTTATTAC ATTAATTACA 
CATGCATTAG GAGGAGAAGC GTATTTAAAT TTTATGGGTA ATGAATTCGG TCATCCTGAA 
TGGTTGGATT TTCCTCGAGC TGGTAACGGG GATAGCTATC ATTATGCTAG AAGGCAATGG 
AATTTAGTTG ACGATGAATT GCTGAAATAT AAGTTTATGA ATAATTGGGA CCGCGCTATA 
AATACTCTTG AAGAAAAATA TGGATGGCTA CATGCTCATC CTGCATATAT AAGTTGGAAA 
CATGAAGGAG ACAAAGTAAT TGTTTTTGAT CGGGCAGAAC TTATATTTGT TTTTAATTTT 
CATCCGGTAC AGTCATTTCC GGATTATCCT ATTGGCGTAA AGACTGCAGG AACTTACAAA 
GTTCTTTTGT GTAGCGACAA TAAGGATTTT GGTGGCGAAA ATCGTGTTGA TACCAGTATA 
CAACATTTTA CCCAACCAGA ATCATTTTCT GATTATTCAA ATAAAATGTT GATTTATATT 
CCTTGTCGCA CAGCTATTGT CTACGTCCGA GAAACATAA