Tpgs1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tpgs1
Entrez Gene ID	110012
Full Name	tubulin polyglutamylase complex subunit 1
Synonyms	AV005629,Gm16517,Gtrgeo22,PGs1
General protein information	Preferred Names tubulin polyglutamylase complex subunit 1 Names tubulin polyglutamylase complex subunit 1 PGs1 protein p32 gene trap ROSA b-geo 22 p32
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10 C1\|10 39.72 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_148934.3	NP_683736.2	tubulin polyglutamylase complex subunit 1
NM_148934.2	NP_683736.1	tubulin polyglutamylase complex subunit 1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	TPGS1	XP_003642907.1
Rattus norvegicus (Norway rat)	Tpgs1	NP_001103091.1
Canis lupus familiaris (dog)	TPGS1	XP_855240.2
Homo sapiens (human)	TPGS1	NP_277048.2
Bos taurus (cattle)	TPGS1	XP_002689183.2
Mus musculus (house mouse)	Tpgs1	NP_683736.1
Xenopus tropicalis (tropical clawed frog)	tpgs1	XP_002939810.1

Gene Ontology
Bibliography

Related articles in PubMed

RNF8/UBC13 ubiquitin signaling suppresses synapse formation in the mammalian brain.
Valnegri P, Huang J, Yamada T, Yang Y, Mejia LA, Cho HY, Oldenborg A, Bonni A
Nature communications8(1)1271(2017 Nov)

Loss of alpha-tubulin polyglutamylation in ROSA22 mice is associated with abnormal targeting of KIF1A and modulated synaptic function.
Ikegami K, Heier RL, Taruishi M, Takagi H, Mukai M, Shimma S, Taira S, Hatanaka K, Morone N, Yao I, Campbell PK, Yuasa S, Janke C, Macgregor GR, Setou M
Proceedings of the National Academy of Sciences of the United States of America104(9)3213-8(2007 Feb)

Tubulin polyglutamylase enzymes are members of the TTL domain protein family.
Janke C, Rogowski K, Wloga D, Regnard C, Kajava AV, Strub JM, Temurak N, van Dijk J, Boucher D, van Dorsselaer A, Suryavanshi S, Gaertig J, Edd? B
Science (New York, N.Y.)308(5729)1758-62(2005 Jun)

Characterisation of PGs1, a subunit of a protein complex co-purifying with tubulin polyglutamylase.
Regnard C, Fesquet D, Janke C, Boucher D, Desbruy?res E, Koulakoff A, Insina C, Travo P, Edd? B
Journal of cell science116(Pt 20)4181-90(2003 Oct)

Mutation of a novel gene results in abnormal development of spermatid flagella, loss of intermale aggression and reduced body fat in mice.
Campbell PK, Waymire KG, Heier RL, Sharer C, Day DE, Reimann H, Jaje JM, Friedrich GA, Burmeister M, Bartness TJ, Russell LD, Young LJ, Zimmer M, Jenne DE, MacGregor GR
Genetics162(1)307-20(2002 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

mutation results in a gross loss of alpha-tubulin polyglutamylation in brain, abnormal intracellular localization of a kinesin motor, KIF1A, and impaired synaptic transmission.
Title: Loss of alpha-tubulin polyglutamylation in ROSA22 mice is associated with abnormal targeting of KIF1A and modulated synaptic function.

constitutes a tubulin polyglutamylase complex.
Title: Tubulin polyglutamylase enzymes are members of the TTL domain protein family.

mutation results in abnormal development of spermatid flagella, loss of intermale aggression and reduced body fat
Title: Mutation of a novel gene results in abnormal development of spermatid flagella, loss of intermale aggression and reduced body fat in mice.

The following Tpgs1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tpgs1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu02075	NM_148934.2 Latest version!	Mus musculus tubulin polyglutamylase complex subunit 1 (Tpgs1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu84894	NM_148934.3 Latest version!	Mus musculus tubulin polyglutamylase complex subunit 1 (Tpgs1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tpgs1 ( NM_148934.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_683736.1 Mus musculus tubulin polyglutamylase complex subunit 1 (Tpgs1), mRNA.

Price & Availability↑

CloneID	OMu02075
Clone ID Related Accession (Same CDS sequence)	NM_148934.2
Accession Version	NM_148934.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Mus musculus(house mouse)
Product	tubulin polyglutamylase complex subunit 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC132265.4. On Sep 6, 2006 this sequence version replaced NM_148934.1. ##Evidence-Data-START## Transcript exon combination :: AF303106.1, AK153467.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCGC TTCCGGTCCC GCTGCCCCCA ACAAAGATGG CGGCAGTGGA GAAACGGCGG 
CCGGCAGTAG CACCAGCGGC CAATTTCCCG GACAGTGGCC GGACGTCGGT GTCTCAGGCG 
GCGGCGGCCG CTGAGAGCGA GGAGGACTTC CTACGGCAGC TCGGCGTGAC GGAGATGCTG 
CGCGCAGCCC TCCTAAAGGT GCTGGAGATC CGGCCCGAGG AGCCCATCGC CTTCTTGGCG 
CACTACTTCG AGAACATGGG TCTACGCTCG CCGACCAACG GAGGCGCCGG GGAGCCCCCG 
GGCCAGCTCC TCCTGCAGCA ACAGCGCCTG GGCCGCGCGC TGTGGCACCT TCGCCTGGCT 
CACCACTCGC AGAGGACCGC CTTCAACAAC AACGTGAGCG TGGCCTACGA GTGTCTGAGT 
GCCAGCGGCC GCAAGAAGAA ACCGGGGCTG GATGGGCGCA CGTACAGCGA GCTGCTGAAG 
CGTGTTTGCA GGGACGGCGG GGCGCCTGAG GAGGTGGTGG CGCCACTGCT TCGCAAGATC 
CAGTGCCGGG ACCATGAGGC TGTGCCTCTG GGCATCTTCA GAACCGGCAT GCTGTCCTGC 
TTTGTGCTGC TGGAATTCGT GGCACGCGCC GGCGCCCTCT TCCAGCTGCT GGAGGACCCT 
GGTCAAGCGG TGGCCGATCG GCGTGTGGGC CAGGCCGTGC TGGACACACT GGAGGGTGCG 
CTGAGCGCAG GAGACATGGC AACAGCACCC ACGCACTACC TGGAGGCCGG TTCGCGCTTG 
GGCCCTGACA ACCTGGCGCG TGCCCTGGAC AGGGCAGCAA GCGGGCGGCG ACCCAGTGTG 
CCCATGGCCC GCGAGGAGTT CCTGGAGAAG GCTGCAGCCC TCTTCATTGC CAAGGTCAAG 
CCAGTGGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_683736.1
CDS	1..912
Translation	MAALPVPLPPTKMAAVEKRRPAVAPAANFPDSGRTSVSQAAAAAESEEDFLRQLGVTEMLRAALLKVLEIRPEEPIAFLAHYFENMGLRSPTNGGAGEPPGQLLLQQQRLGRALWHLRLAHHSQRTAFNNNVSVAYECLSASGRKKKPGLDGRTYSELLKRVCRDGGAPEEVVAPLLRKIQCRDHEAVPLGIFRTGMLSCFVLLEFVARAGALFQLLEDPGQAVADRRVGQAVLDTLEGALSAGDMATAPTHYLEAGSRLGPDNLARALDRAASGRRPSVPMAREEFLEKAAALFIAKVKPVG

Target ORF information:

RefSeq Version	NM_148934.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tubulin polyglutamylase complex subunit 1 (Tpgs1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_148934.2

ORF Insert Sequence:

ATGGCGGCGC TTCCGGTCCC GCTGCCCCCA ACAAAGATGG CGGCAGTGGA GAAACGGCGG 
CCGGCAGTAG CACCAGCGGC CAATTTCCCG GACAGTGGCC GGACGTCGGT GTCTCAGGCG 
GCGGCGGCCG CTGAGAGCGA GGAGGACTTC CTACGGCAGC TCGGCGTGAC GGAGATGCTG 
CGCGCAGCCC TCCTAAAGGT GCTGGAGATC CGGCCCGAGG AGCCCATCGC CTTCTTGGCG 
CACTACTTCG AGAACATGGG TCTACGCTCG CCGACCAACG GAGGCGCCGG GGAGCCCCCG 
GGCCAGCTCC TCCTGCAGCA ACAGCGCCTG GGCCGCGCGC TGTGGCACCT TCGCCTGGCT 
CACCACTCGC AGAGGACCGC CTTCAACAAC AACGTGAGCG TGGCCTACGA GTGTCTGAGT 
GCCAGCGGCC GCAAGAAGAA ACCGGGGCTG GATGGGCGCA CGTACAGCGA GCTGCTGAAG 
CGTGTTTGCA GGGACGGCGG GGCGCCTGAG GAGGTGGTGG CGCCACTGCT TCGCAAGATC 
CAGTGCCGGG ACCATGAGGC TGTGCCTCTG GGCATCTTCA GAACCGGCAT GCTGTCCTGC 
TTTGTGCTGC TGGAATTCGT GGCACGCGCC GGCGCCCTCT TCCAGCTGCT GGAGGACCCT 
GGTCAAGCGG TGGCCGATCG GCGTGTGGGC CAGGCCGTGC TGGACACACT GGAGGGTGCG 
CTGAGCGCAG GAGACATGGC AACAGCACCC ACGCACTACC TGGAGGCCGG TTCGCGCTTG 
GGCCCTGACA ACCTGGCGCG TGCCCTGGAC AGGGCAGCAA GCGGGCGGCG ACCCAGTGTG 
CCCATGGCCC GCGAGGAGTT CCTGGAGAAG GCTGCAGCCC TCTTCATTGC CAAGGTCAAG 
CCAGTGGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tpgs1 ( NM_148934.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_683736.2 Mus musculus tubulin polyglutamylase complex subunit 1 (Tpgs1), mRNA.

Price & Availability↑

CloneID	OMu84894
Clone ID Related Accession (Same CDS sequence)	NM_148934.3
Accession Version	NM_148934.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-19
Organism	Mus musculus(house mouse)
Product	tubulin polyglutamylase complex subunit 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC132265.4. On Jun 20, 2019 this sequence version replaced NM_148934.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR7345562.1856124.1, SRR7345562.1832000.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCAG TGGAGAAACG GCGGCCGGCA GTAGCACCAG CGGCCAATTT CCCGGACAGT 
GGCCGGACGT CGGTGTCTCA GGCGGCGGCG GCCGCTGAGA GCGAGGAGGA CTTCCTACGG 
CAGCTCGGCG TGACGGAGAT GCTGCGCGCA GCCCTCCTAA AGGTGCTGGA GATCCGGCCC 
GAGGAGCCCA TCGCCTTCTT GGCGCACTAC TTCGAGAACA TGGGTCTACG CTCGCCGACC 
AACGGAGGCG CCGGGGAGCC CCCGGGCCAG CTCCTCCTGC AGCAACAGCG CCTGGGCCGC 
GCGCTGTGGC ACCTTCGCCT GGCTCACCAC TCGCAGAGGA CCGCCTTCAA CAACAACGTG 
AGCGTGGCCT ACGAGTGTCT GAGTGCCAGC GGCCGCAAGA AGAAACCGGG GCTGGATGGG 
CGCACGTACA GCGAGCTGCT GAAGCGTGTT TGCAGGGACG GCGGGGCGCC TGAGGAGGTG 
GTGGCGCCAC TGCTTCGCAA GATCCAGTGC CGGGACCATG AGGCTGTGCC TCTGGGCATC 
TTCAGAACCG GCATGCTGTC CTGCTTTGTG CTGCTGGAAT TCGTGGCACG CGCCGGCGCC 
CTCTTCCAGC TGCTGGAGGA CCCTGGTCAA GCGGTGGCCG ATCGGCGTGT GGGCCAGGCC 
GTGCTGGACA CACTGGAGGG TGCGCTGAGC GCAGGAGACA TGGCAACAGC ACCCACGCAC 
TACCTGGAGG CCGGTTCGCG CTTGGGCCCT GACAACCTGG CGCGTGCCCT GGACAGGGCA 
GCAAGCGGGC GGCGACCCAG TGTGCCCATG GCCCGCGAGG AGTTCCTGGA GAAGGCTGCA 
GCCCTCTTCA TTGCCAAGGT CAAGCCAGTG GGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_683736.2
CDS	8..883
Translation	MAAVEKRRPAVAPAANFPDSGRTSVSQAAAAAESEEDFLRQLGVTEMLRAALLKVLEIRPEEPIAFLAHYFENMGLRSPTNGGAGEPPGQLLLQQQRLGRALWHLRLAHHSQRTAFNNNVSVAYECLSASGRKKKPGLDGRTYSELLKRVCRDGGAPEEVVAPLLRKIQCRDHEAVPLGIFRTGMLSCFVLLEFVARAGALFQLLEDPGQAVADRRVGQAVLDTLEGALSAGDMATAPTHYLEAGSRLGPDNLARALDRAASGRRPSVPMAREEFLEKAAALFIAKVKPVG

Target ORF information:

RefSeq Version	NM_148934.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tubulin polyglutamylase complex subunit 1 (Tpgs1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_148934.3

ORF Insert Sequence:

ATGGCGGCAG TGGAGAAACG GCGGCCGGCA GTAGCACCAG CGGCCAATTT CCCGGACAGT 
GGCCGGACGT CGGTGTCTCA GGCGGCGGCG GCCGCTGAGA GCGAGGAGGA CTTCCTACGG 
CAGCTCGGCG TGACGGAGAT GCTGCGCGCA GCCCTCCTAA AGGTGCTGGA GATCCGGCCC 
GAGGAGCCCA TCGCCTTCTT GGCGCACTAC TTCGAGAACA TGGGTCTACG CTCGCCGACC 
AACGGAGGCG CCGGGGAGCC CCCGGGCCAG CTCCTCCTGC AGCAACAGCG CCTGGGCCGC 
GCGCTGTGGC ACCTTCGCCT GGCTCACCAC TCGCAGAGGA CCGCCTTCAA CAACAACGTG 
AGCGTGGCCT ACGAGTGTCT GAGTGCCAGC GGCCGCAAGA AGAAACCGGG GCTGGATGGG 
CGCACGTACA GCGAGCTGCT GAAGCGTGTT TGCAGGGACG GCGGGGCGCC TGAGGAGGTG 
GTGGCGCCAC TGCTTCGCAA GATCCAGTGC CGGGACCATG AGGCTGTGCC TCTGGGCATC 
TTCAGAACCG GCATGCTGTC CTGCTTTGTG CTGCTGGAAT TCGTGGCACG CGCCGGCGCC 
CTCTTCCAGC TGCTGGAGGA CCCTGGTCAA GCGGTGGCCG ATCGGCGTGT GGGCCAGGCC 
GTGCTGGACA CACTGGAGGG TGCGCTGAGC GCAGGAGACA TGGCAACAGC ACCCACGCAC 
TACCTGGAGG CCGGTTCGCG CTTGGGCCCT GACAACCTGG CGCGTGCCCT GGACAGGGCA 
GCAAGCGGGC GGCGACCCAG TGTGCCCATG GCCCGCGAGG AGTTCCTGGA GAAGGCTGCA 
GCCCTCTTCA TTGCCAAGGT CAAGCCAGTG GGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RNF8/UBC13 ubiquitin signaling suppresses synapse formation in the mammalian brain.
Nature communications8(1)1271(2017 Nov)
Valnegri P,Huang J,Yamada T,Yang Y,Mejia LA,Cho HY,Oldenborg A,Bonni A

Loss of alpha-tubulin polyglutamylation in ROSA22 mice is associated with abnormal targeting of KIF1A and modulated synaptic function.
Proceedings of the National Academy of Sciences of the United States of America104(9)3213-8(2007 Feb)
Ikegami K,Heier RL,Taruishi M,Takagi H,Mukai M,Shimma S,Taira S,Hatanaka K,Morone N,Yao I,Campbell PK,Yuasa S,Janke C,Macgregor GR,Setou M

Tubulin polyglutamylase enzymes are members of the TTL domain protein family.
Science (New York, N.Y.)308(5729)1758-62(2005 Jun)
Janke C,Rogowski K,Wloga D,Regnard C,Kajava AV,Strub JM,Temurak N,van Dijk J,Boucher D,van Dorsselaer A,Suryavanshi S,Gaertig J,Edd? B

Characterisation of PGs1, a subunit of a protein complex co-purifying with tubulin polyglutamylase.
Journal of cell science116(Pt 20)4181-90(2003 Oct)
Regnard C,Fesquet D,Janke C,Boucher D,Desbruy?res E,Koulakoff A,Insina C,Travo P,Edd? B

Mutation of a novel gene results in abnormal development of spermatid flagella, loss of intermale aggression and reduced body fat in mice.
Genetics162(1)307-20(2002 Sep)
Campbell PK,Waymire KG,Heier RL,Sharer C,Day DE,Reimann H,Jaje JM,Friedrich GA,Burmeister M,Bartness TJ,Russell LD,Young LJ,Zimmer M,Jenne DE,MacGregor GR

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Tpgs1 cDNA ORF clone, Mus musculus(house mouse)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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