Dbi cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dbi
Entrez Gene ID	13167
Full Name	diazepam binding inhibitor
Synonyms	ACBD1,Acbp,EP,endozepine
General protein information	Preferred Names diazepam binding inhibitor Names acyl-CoA-binding protein diazepam binding inhibitor, splice form 1b
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 E2.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_007830.4	NP_031856.1	acyl-CoA-binding protein isoform 2
NM_001037999.2	NP_001033088.1	acyl-CoA-binding protein isoform 1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	DBI	NP_989907.1
Saccharomyces cerevisiae (baker's yeast)	ACB1	NP_011551.3
Schizosaccharomyces pombe (fission yeast)	SPBC1539.06	NP_596820.1
Caenorhabditis elegans (roundworm)	acbp-1	NP_491412.1
Kluyveromyces lactis	KLLA0B05643g	XP_451787.2
Eremothecium gossypii	AGOS_ACL188W	NP_983216.1
Mus musculus (house mouse)	Dbi	NP_031856.1
Rattus norvegicus (Norway rat)	Dbi	NP_114054.1
Drosophila melanogaster (fruit fly)	Dbi	NP_523952.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007460	XP_001687920.1
Homo sapiens (human)	DBI	NP_001073331.1
	DBI	XP_002799453.1
Danio rerio (zebrafish)	dbi	NP_955902.1
Pan troglodytes (chimpanzee)	DBI	XP_515759.2
Bos taurus (cattle)	DBI	NP_001106792.1

Gene Ontology
Bibliography

Related articles in PubMed

Analogous mechanism regulating formation of neocortical basal radial glia and cerebellar Bergmann glia.
Heng X, Guo Q, Leung AW, Li JY
eLife6(2017 May)

Regulation of very-long acyl chain ceramide synthesis by acyl-CoA-binding protein.
Ferreira NS, Engelsby H, Neess D, Kelly SL, Volpert G, Merrill AH, Futerman AH, F?rgeman NJ
The Journal of biological chemistry292(18)7588-7597(2017 05)

Diazepam Binding Inhibitor Promotes Stem Cell Expansion Controlling Environment-Dependent Neurogenesis.
Dumitru I, Neitz A, Alfonso J, Monyer H
Neuron94(1)125-137.e5(2017 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ACBP increases the activity of ceramide synthase 2 (CerS2) by more than 2-fold and CerS3 activity by 7-fold. ACBP binds very-long-chain acyl-CoA esters, which is required for its ability to stimulate CerS activity.
Title: Regulation of very-long acyl chain ceramide synthesis by acyl-CoA-binding protein.

DBI is expressed in stem cells in all neurogenic niches of the postnatal brain. DBI regulates the balance between preserving the stem cell pool and neurogenesis. Specifically, DBI dampens GABA activity in stem cells, thereby sustaining the proproliferative effect of physical exercise and enriched environment.
Title: Diazepam Binding Inhibitor Promotes Stem Cell Expansion Controlling Environment-Dependent Neurogenesis.

Mice with conditional targeting of the Acbp gene in the epidermis recapitulate this phenotype, whereas generation of an artificial epidermal barrier during gland development reverses the phenotype
Title: Compromised epidermal barrier stimulates Harderian gland activity and hypertrophy in ACBP-/- mice.

This study demonistrated that a novel role for ACBP in brain lipid metabolism.
Title: A novel role for central ACBP/DBI as a regulator of long-chain fatty acid metabolism in astrocytes.

Acyl-CoA binding protein and epidermal barrier function. [review]
Title: Acyl-CoA binding protein and epidermal barrier function.

The following Dbi gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dbi cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu18309	NM_001037999.2 Latest version!	Mus musculus diazepam binding inhibitor (Dbi), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu18383	NM_007830.4 Latest version!	Mus musculus diazepam binding inhibitor (Dbi), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dbi ( NM_001037999.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001033088.1 Mus musculus diazepam binding inhibitor (Dbi), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu18309
Clone ID Related Accession (Same CDS sequence)	NM_001037999.2
Accession Version	NM_001037999.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	acyl-CoA-binding protein isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC139867.4. On Mar 17, 2010 this sequence version replaced NM_001037999.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AM000002.1, AA492713.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCTGTGGG TTTGGGGACA GCCGGGTCTA TTTCCTCAGA TATCCCGCAG CTCTTCTGGG 
ATCCGAGCCC AGCTCATAGC CTGGGGAGCG GAACTTTTTC CCTTGCAGAT ATGTAAAACT 
GCCACCCACG CCGGAACTGC CAGAGAGTTG CCGGCTGAAT TTGACAAAGC CGCTGAGGAG 
GTGAAGCGCC TCAAGACTCA GCCAACTGAT GAAGAGATGC TGTTCATCTA CAGTCACTTC 
AAACAAGCTA CTGTGGGCGA TGTAAATACA GATCGGCCGG GGCTCTTGGA CCTCAAGGGC 
AAAGCCAAGT GGGACTCGTG GAACAAGCTG AAAGGGACTT CCAAGGAAAG TGCCATGAAG 
ACCTATGTGG AAAAGGTAGA CGAGCTAAAG AAGAAATACG GAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001033088.1
CDS	6..413
Translation	MLWVWGQPGLFPQISRSSSGIRAQLIAWGAELFPLQICKTATHAGTARELPAEFDKAAEEVKRLKTQPTDEEMLFIYSHFKQATVGDVNTDRPGLLDLKGKAKWDSWNKLKGTSKESAMKTYVEKVDELKKKYGI

Target ORF information:

RefSeq Version	NM_001037999.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus diazepam binding inhibitor (Dbi), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001037999.2

ORF Insert Sequence:

ATGCTGTGGG TTTGGGGACA GCCGGGTCTA TTTCCTCAGA TATCCCGCAG CTCTTCTGGG 
ATCCGAGCCC AGCTCATAGC CTGGGGAGCG GAACTTTTTC CCTTGCAGAT ATGTAAAACT 
GCCACCCACG CCGGAACTGC CAGAGAGTTG CCGGCTGAAT TTGACAAAGC CGCTGAGGAG 
GTGAAGCGCC TCAAGACTCA GCCAACTGAT GAAGAGATGC TGTTCATCTA CAGTCACTTC 
AAACAAGCTA CTGTGGGCGA TGTAAATACA GATCGGCCGG GGCTCTTGGA CCTCAAGGGC 
AAAGCCAAGT GGGACTCGTG GAACAAGCTG AAAGGGACTT CCAAGGAAAG TGCCATGAAG 
ACCTATGTGG AAAAGGTAGA CGAGCTAAAG AAGAAATACG GAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dbi ( NM_007830.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031856.1 Mus musculus diazepam binding inhibitor (Dbi), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu18383
Clone ID Related Accession (Same CDS sequence)	NM_007830.4
Accession Version	NM_007830.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	acyl-CoA-binding protein isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ183794.1, AK020692.1 and AV001592.1. On Mar 17, 2010 this sequence version replaced NM_007830.3. Transcript Variant: This variant (2) has an alternate 5' exon, as compared to variant 1. The resulting isoform (2) is shorter and has a different N-terminus, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AV001592.1, AV574848.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGTCTCAGG CTGAATTTGA CAAAGCCGCT GAGGAGGTGA AGCGCCTCAA GACTCAGCCA 
ACTGATGAAG AGATGCTGTT CATCTACAGT CACTTCAAAC AAGCTACTGT GGGCGATGTA 
AATACAGATC GGCCGGGGCT CTTGGACCTC AAGGGCAAAG CCAAGTGGGA CTCGTGGAAC 
AAGCTGAAAG GGACTTCCAA GGAAAGTGCC ATGAAGACCT ATGTGGAAAA GGTAGACGAG 
CTAAAGAAGA AATACGGAAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031856.1
CDS	144..407
Translation	MSQAEFDKAAEEVKRLKTQPTDEEMLFIYSHFKQATVGDVNTDRPGLLDLKGKAKWDSWNKLKGTSKESAMKTYVEKVDELKKKYGI

Target ORF information:

RefSeq Version	NM_007830.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus diazepam binding inhibitor (Dbi), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007830.4

ORF Insert Sequence:

ATGTCTCAGG CTGAATTTGA CAAAGCCGCT GAGGAGGTGA AGCGCCTCAA GACTCAGCCA 
ACTGATGAAG AGATGCTGTT CATCTACAGT CACTTCAAAC AAGCTACTGT GGGCGATGTA 
AATACAGATC GGCCGGGGCT CTTGGACCTC AAGGGCAAAG CCAAGTGGGA CTCGTGGAAC 
AAGCTGAAAG GGACTTCCAA GGAAAGTGCC ATGAAGACCT ATGTGGAAAA GGTAGACGAG 
CTAAAGAAGA AATACGGAAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Analogous mechanism regulating formation of neocortical basal radial glia and cerebellar Bergmann glia.
eLife6(2017 May)
Heng X,Guo Q,Leung AW,Li JY

Regulation of very-long acyl chain ceramide synthesis by acyl-CoA-binding protein.
The Journal of biological chemistry292(18)7588-7597(2017 05)
Ferreira NS,Engelsby H,Neess D,Kelly SL,Volpert G,Merrill AH,Futerman AH,F?rgeman NJ

Diazepam Binding Inhibitor Promotes Stem Cell Expansion Controlling Environment-Dependent Neurogenesis.
Neuron94(1)125-137.e5(2017 Apr)
Dumitru I,Neitz A,Alfonso J,Monyer H

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Dbi cDNA ORF clone, Mus musculus(house mouse)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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