Grap2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Grap2
Entrez Gene ID	17444
Full Name	GRB2-related adaptor protein 2
Synonyms	GRAP-2,GRB2L,GRBLG,GRID,Gads,GrbX,Grf40,GrpL,Mona,P38
General protein information	Preferred Names GRB2-related adaptor protein 2 Names GRB2-related adaptor protein 2 GRB-2-like protein GRB-2-related monocytic adapter protein Grb2-related adaptor downstream of Sch adapter protein GRID growth factor receptor-binding protein hematopoietic cell-associated adaptor protein GrpL monocytic adapter monocytic adaptor
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15\|15 E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001289442.1	NP_001276371.1	GRB2-related adaptor protein 2
NM_010815.3	NP_034945.1	GRB2-related adaptor protein 2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Grap2	NP_001030116.1
Gallus gallus (chicken)	GRAP2	XP_004937789.1
Danio rerio (zebrafish)	grap2b	NP_956972.1
Canis lupus familiaris (dog)	GRAP2	XP_005625915.1
Mus musculus (house mouse)	Grap2	NP_034945.1
Xenopus tropicalis (tropical clawed frog)	grap2	XP_002933793.1
Homo sapiens (human)	GRAP2	NP_004801.1
	GRAP2	XP_001100458.2
Bos taurus (cattle)	GRAP2	NP_001179489.1
Danio rerio (zebrafish)	grap2a	NP_001017756.1
Pan troglodytes (chimpanzee)	GRAP2	XP_001166508.1

Gene Ontology
Bibliography

Related articles in PubMed

Expression of GADS enhances FLT3-induced mitogenic signaling.
Chougule RA, Cordero E, Moharram SA, Pietras K, R?nnstrand L, Kazi JU
Oncotarget7(12)14112-24(2016 Mar)

The ubiquitin-specific protease USP8 is critical for the development and homeostasis of T cells.
Dufner A, Kisser A, Niendorf S, Basters A, Reissig S, Sch?nle A, Aichem A, Kurz T, Schlosser A, Yablonski D, Groettrup M, Buch T, Waisman A, Schamel WW, Prinz M, Knobeloch KP
Nature immunology16(9)950-60(2015 Sep)

The combined loss of Gads and CD127 reveals a novel function of Gads prior to TCR? expression.
Xiong J, Parker BL, Yankee TM
Immunologic research60(1)77-84(2014 Oct)

Quantitative proteomics analysis of signalosome dynamics in primary T cells identifies the surface receptor CD6 as a Lat adaptor-independent TCR signaling hub.
Roncagalli R, Hauri S, Fiore F, Liang Y, Chen Z, Sansoni A, Kanduri K, Joly R, Malzac A, L?hdesm?ki H, Lahesmaa R, Yamasaki S, Saito T, Malissen M, Aebersold R, Gstaiger M, Malissen B
Nature immunology15(4)384-392(2014 Apr)

Gads (Grb2-related adaptor downstream of Shc) is required for BCR-ABL-mediated lymphoid leukemia.
Gillis LC, Berry DM, Minden MD, McGlade CJ, Barber DL
Leukemia27(8)1666-76(2013 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

High GADS expression is associated with neoplasms.
Title: Expression of GADS enhances FLT3-induced mitogenic signaling.

The combined loss of Gads and CD127 reveals a novel function of Gads prior to T cell receptor beta expression
Title: The combined loss of Gads and CD127 reveals a novel function of Gads prior to TCR? expression.

GADS mediates lymphoid disease downstream of BCR-ABL through the recruitment of specific signaling intermediates.
Title: Gads (Grb2-related adaptor downstream of Shc) is required for BCR-ABL-mediated lymphoid leukemia.

Data show that the primary function of Gads is to regulate the sensitivity of the TCR to Ag ligation.
Title: Gads regulates the expansion phase of CD8+ T cell-mediated immunity.

Gads plays a key role in linking the adapter LAT to SLP-76 in response to weak activation of GPVI and CLEC-2 whereas LAT is required for full activation over a wider range of agonist concentrations.
Title: Differential roles for the adapters Gads and LAT in platelet activation by GPVI and CLEC-2.

The following Grap2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Grap2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu14074	NM_010815.3 Latest version!	Mus musculus GRB2-related adaptor protein 2 (Grap2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu14074	NM_001289442.1 Latest version!	Mus musculus GRB2-related adaptor protein 2 (Grap2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Grap2 ( NM_001289442.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001276371.1 Mus musculus GRB2-related adaptor protein 2 (Grap2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu14074
Clone ID Related Accession (Same CDS sequence)	NM_001289442.1 , NM_010815.3
Accession Version	NM_001289442.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Mus musculus(house mouse)
Product	GRB2-related adaptor protein 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC052496.1 and AC147041.3. On Jan 22, 2014 this sequence version replaced XM_006520559.1. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC052496.1, AK156939.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849377 [ECO:0000348] ##Evidence-Data-END##

ATGGAAGCCA CTGCCAAGTT TGATTTCATG GCCTCAGGTG AGGACGAACT GAGCTTTCGC 
ACTGGAGACA TTCTGAAGAT ACTAAGCAAC CAGGAGGAGT GGCTCAAGGC GGAGCTCGGA 
AGCCAAGAAG GATATGTGCC TAAGAATTTT ATAGATATCG AGTTTCCTGA GTGGTTCCAT 
GAAGGCCTCT CTCGACATCA AGCAGAAAAC TTACTCATGG GCAAGGACAT TGGATTCTTC 
ATCATCAGGG CCAGCCAGAG TTCCCCAGGA GACTTCTCCA TCTCTGTCAG GCACGAGGAC 
GACGTTCAGC ACTTCAAAGT CATGCGTGAC ACCAAGGGCA ATTACTTCCT GTGGACCGAG 
AAGTTCCCAT CCTTAAATAA GCTGGTGGAC TACTACCGGA CAACGTCCAT CTCCAAACAG 
AAGCAGGTCT TCCTTCGGGA TGGAACCCAA GATCAGGGTC ACCGGGGCAA CAGCCTGGAC 
AGGAGGTCCC AGGGCGGCCC TCACCCGAGT GGAACCGTAG GAGAAGAAAT CCGACCTTCA 
GTGAACCGGA AGCTGTCAGA CCACCTTCCT CTGGGTCCCC AGCAGTTCCA CCCGCACCAG 
CAGCCGTCAC CCCAGTTCAC ACCAGGGCCG CAGCCCCCTC AACAGCAGCG TTATCTGCAG 
CATTTCCACC AGGACCGTCG TGGGGGCAGC CTGGACATAA ACGATGGACA CTGCGGCCTT 
GGAAGTGAGG TGAACGCCAC GCTGATGCAC CGGAGACACA CCGACCCCGT ACAACTCCAG 
GCAGCAGGGC GGGTGCGTTG GGCCAGGGCA CTGTATGACT TTGAGGCTCT GGAAGAGGAC 
GAGCTGGGAT TCCGAAGCGG AGAAGTGGTT GAAGTCCTGG ACAGCTCCAA CCCATCTTGG 
TGGACCGGCC GTCTGCACAA CAAACTGGGT CTCTTCCCTG CCAACTATGT GGCCCCCATG 
ATGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276371.1
CDS	160..1128
Translation	MEATAKFDFMASGEDELSFRTGDILKILSNQEEWLKAELGSQEGYVPKNFIDIEFPEWFHEGLSRHQAENLLMGKDIGFFIIRASQSSPGDFSISVRHEDDVQHFKVMRDTKGNYFLWTEKFPSLNKLVDYYRTTSISKQKQVFLRDGTQDQGHRGNSLDRRSQGGPHPSGTVGEEIRPSVNRKLSDHLPLGPQQFHPHQQPSPQFTPGPQPPQQQRYLQHFHQDRRGGSLDINDGHCGLGSEVNATLMHRRHTDPVQLQAAGRVRWARALYDFEALEEDELGFRSGEVVEVLDSSNPSWWTGRLHNKLGLFPANYVAPMMR

Target ORF information:

RefSeq Version	NM_001289442.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus GRB2-related adaptor protein 2 (Grap2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289442.1

ORF Insert Sequence:

ATGGAAGCCA CTGCCAAGTT TGATTTCATG GCCTCAGGTG AGGACGAACT GAGCTTTCGC 
ACTGGAGACA TTCTGAAGAT ACTAAGCAAC CAGGAGGAGT GGCTCAAGGC GGAGCTCGGA 
AGCCAAGAAG GATATGTGCC TAAGAATTTT ATAGATATCG AGTTTCCTGA GTGGTTCCAT 
GAAGGCCTCT CTCGACATCA AGCAGAAAAC TTACTCATGG GCAAGGACAT TGGATTCTTC 
ATCATCAGGG CCAGCCAGAG TTCCCCAGGA GACTTCTCCA TCTCTGTCAG GCACGAGGAC 
GACGTTCAGC ACTTCAAAGT CATGCGTGAC ACCAAGGGCA ATTACTTCCT GTGGACCGAG 
AAGTTCCCAT CCTTAAATAA GCTGGTGGAC TACTACCGGA CAACGTCCAT CTCCAAACAG 
AAGCAGGTCT TCCTTCGGGA TGGAACCCAA GATCAGGGTC ACCGGGGCAA CAGCCTGGAC 
AGGAGGTCCC AGGGCGGCCC TCACCCGAGT GGAACCGTAG GAGAAGAAAT CCGACCTTCA 
GTGAACCGGA AGCTGTCAGA CCACCTTCCT CTGGGTCCCC AGCAGTTCCA CCCGCACCAG 
CAGCCGTCAC CCCAGTTCAC ACCAGGGCCG CAGCCCCCTC AACAGCAGCG TTATCTGCAG 
CATTTCCACC AGGACCGTCG TGGGGGCAGC CTGGACATAA ACGATGGACA CTGCGGCCTT 
GGAAGTGAGG TGAACGCCAC GCTGATGCAC CGGAGACACA CCGACCCCGT ACAACTCCAG 
GCAGCAGGGC GGGTGCGTTG GGCCAGGGCA CTGTATGACT TTGAGGCTCT GGAAGAGGAC 
GAGCTGGGAT TCCGAAGCGG AGAAGTGGTT GAAGTCCTGG ACAGCTCCAA CCCATCTTGG 
TGGACCGGCC GTCTGCACAA CAAACTGGGT CTCTTCCCTG CCAACTATGT GGCCCCCATG 
ATGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Grap2 ( NM_010815.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034945.1 Mus musculus GRB2-related adaptor protein 2 (Grap2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu14074
Clone ID Related Accession (Same CDS sequence)	NM_001289442.1 , NM_010815.3
Accession Version	NM_010815.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Mus musculus(house mouse)
Product	GRB2-related adaptor protein 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC147041.3. On Jan 22, 2014 this sequence version replaced NM_010815.2. Transcript Variant: This variant (1) represents the longer variant. Variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF055465.1, AK156939.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849377 [ECO:0000348] ##Evidence-Data-END##

ATGGAAGCCA CTGCCAAGTT TGATTTCATG GCCTCAGGTG AGGACGAACT GAGCTTTCGC 
ACTGGAGACA TTCTGAAGAT ACTAAGCAAC CAGGAGGAGT GGCTCAAGGC GGAGCTCGGA 
AGCCAAGAAG GATATGTGCC TAAGAATTTT ATAGATATCG AGTTTCCTGA GTGGTTCCAT 
GAAGGCCTCT CTCGACATCA AGCAGAAAAC TTACTCATGG GCAAGGACAT TGGATTCTTC 
ATCATCAGGG CCAGCCAGAG TTCCCCAGGA GACTTCTCCA TCTCTGTCAG GCACGAGGAC 
GACGTTCAGC ACTTCAAAGT CATGCGTGAC ACCAAGGGCA ATTACTTCCT GTGGACCGAG 
AAGTTCCCAT CCTTAAATAA GCTGGTGGAC TACTACCGGA CAACGTCCAT CTCCAAACAG 
AAGCAGGTCT TCCTTCGGGA TGGAACCCAA GATCAGGGTC ACCGGGGCAA CAGCCTGGAC 
AGGAGGTCCC AGGGCGGCCC TCACCCGAGT GGAACCGTAG GAGAAGAAAT CCGACCTTCA 
GTGAACCGGA AGCTGTCAGA CCACCTTCCT CTGGGTCCCC AGCAGTTCCA CCCGCACCAG 
CAGCCGTCAC CCCAGTTCAC ACCAGGGCCG CAGCCCCCTC AACAGCAGCG TTATCTGCAG 
CATTTCCACC AGGACCGTCG TGGGGGCAGC CTGGACATAA ACGATGGACA CTGCGGCCTT 
GGAAGTGAGG TGAACGCCAC GCTGATGCAC CGGAGACACA CCGACCCCGT ACAACTCCAG 
GCAGCAGGGC GGGTGCGTTG GGCCAGGGCA CTGTATGACT TTGAGGCTCT GGAAGAGGAC 
GAGCTGGGAT TCCGAAGCGG AGAAGTGGTT GAAGTCCTGG ACAGCTCCAA CCCATCTTGG 
TGGACCGGCC GTCTGCACAA CAAACTGGGT CTCTTCCCTG CCAACTATGT GGCCCCCATG 
ATGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034945.1
CDS	255..1223
Translation	MEATAKFDFMASGEDELSFRTGDILKILSNQEEWLKAELGSQEGYVPKNFIDIEFPEWFHEGLSRHQAENLLMGKDIGFFIIRASQSSPGDFSISVRHEDDVQHFKVMRDTKGNYFLWTEKFPSLNKLVDYYRTTSISKQKQVFLRDGTQDQGHRGNSLDRRSQGGPHPSGTVGEEIRPSVNRKLSDHLPLGPQQFHPHQQPSPQFTPGPQPPQQQRYLQHFHQDRRGGSLDINDGHCGLGSEVNATLMHRRHTDPVQLQAAGRVRWARALYDFEALEEDELGFRSGEVVEVLDSSNPSWWTGRLHNKLGLFPANYVAPMMR

Target ORF information:

RefSeq Version	NM_010815.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus GRB2-related adaptor protein 2 (Grap2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010815.3

ORF Insert Sequence:

ATGGAAGCCA CTGCCAAGTT TGATTTCATG GCCTCAGGTG AGGACGAACT GAGCTTTCGC 
ACTGGAGACA TTCTGAAGAT ACTAAGCAAC CAGGAGGAGT GGCTCAAGGC GGAGCTCGGA 
AGCCAAGAAG GATATGTGCC TAAGAATTTT ATAGATATCG AGTTTCCTGA GTGGTTCCAT 
GAAGGCCTCT CTCGACATCA AGCAGAAAAC TTACTCATGG GCAAGGACAT TGGATTCTTC 
ATCATCAGGG CCAGCCAGAG TTCCCCAGGA GACTTCTCCA TCTCTGTCAG GCACGAGGAC 
GACGTTCAGC ACTTCAAAGT CATGCGTGAC ACCAAGGGCA ATTACTTCCT GTGGACCGAG 
AAGTTCCCAT CCTTAAATAA GCTGGTGGAC TACTACCGGA CAACGTCCAT CTCCAAACAG 
AAGCAGGTCT TCCTTCGGGA TGGAACCCAA GATCAGGGTC ACCGGGGCAA CAGCCTGGAC 
AGGAGGTCCC AGGGCGGCCC TCACCCGAGT GGAACCGTAG GAGAAGAAAT CCGACCTTCA 
GTGAACCGGA AGCTGTCAGA CCACCTTCCT CTGGGTCCCC AGCAGTTCCA CCCGCACCAG 
CAGCCGTCAC CCCAGTTCAC ACCAGGGCCG CAGCCCCCTC AACAGCAGCG TTATCTGCAG 
CATTTCCACC AGGACCGTCG TGGGGGCAGC CTGGACATAA ACGATGGACA CTGCGGCCTT 
GGAAGTGAGG TGAACGCCAC GCTGATGCAC CGGAGACACA CCGACCCCGT ACAACTCCAG 
GCAGCAGGGC GGGTGCGTTG GGCCAGGGCA CTGTATGACT TTGAGGCTCT GGAAGAGGAC 
GAGCTGGGAT TCCGAAGCGG AGAAGTGGTT GAAGTCCTGG ACAGCTCCAA CCCATCTTGG 
TGGACCGGCC GTCTGCACAA CAAACTGGGT CTCTTCCCTG CCAACTATGT GGCCCCCATG 
ATGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression of GADS enhances FLT3-induced mitogenic signaling.
Oncotarget7(12)14112-24(2016 Mar)
Chougule RA,Cordero E,Moharram SA,Pietras K,R?nnstrand L,Kazi JU

The ubiquitin-specific protease USP8 is critical for the development and homeostasis of T cells.
Nature immunology16(9)950-60(2015 Sep)
Dufner A,Kisser A,Niendorf S,Basters A,Reissig S,Sch?nle A,Aichem A,Kurz T,Schlosser A,Yablonski D,Groettrup M,Buch T,Waisman A,Schamel WW,Prinz M,Knobeloch KP

The combined loss of Gads and CD127 reveals a novel function of Gads prior to TCR? expression.
Immunologic research60(1)77-84(2014 Oct)
Xiong J,Parker BL,Yankee TM

Quantitative proteomics analysis of signalosome dynamics in primary T cells identifies the surface receptor CD6 as a Lat adaptor-independent TCR signaling hub.
Nature immunology15(4)384-392(2014 Apr)
Roncagalli R,Hauri S,Fiore F,Liang Y,Chen Z,Sansoni A,Kanduri K,Joly R,Malzac A,L?hdesm?ki H,Lahesmaa R,Yamasaki S,Saito T,Malissen M,Aebersold R,Gstaiger M,Malissen B

Gads (Grb2-related adaptor downstream of Shc) is required for BCR-ABL-mediated lymphoid leukemia.
Leukemia27(8)1666-76(2013 Aug)
Gillis LC,Berry DM,Minden MD,McGlade CJ,Barber DL