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MYC cDNA ORF clone, Mus musculus(House mouse)

Gene Symbol MYC
Entrez Gene ID 17869
Full Name myelocytomatosis oncogene
Synonyms AU016757,Myc2,Niard,Nird,bHLHe39
General protein information
Preferred Names

myelocytomatosis oncogene

Names

myc proto-oncogene protein
c-myc proto-oncogene
transcription factor p64

Gene Type protein-coding
Organism Mus musculus(House mouse)
Genome

15

15 D1|15 26.19 cM

Summary The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma, in human. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini, in human and mouse. Under conditions of stress, such as high cell densities and methionine deprivation, there is a specific and dramatic increase in the synthesis of the non-AUG initiated protein, suggesting its importance in times of adversity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010].

mRNA and Protein(s)

mRNA Protein Name
NM_001177353.1 NP_001170824.1 myc proto-oncogene protein isoform c
NM_010849.4 NP_034979.3 myc proto-oncogene protein isoform a
NM_001177354.1 NP_001170825.1 myc proto-oncogene protein isoform b
NM_001177352.1 NP_001170823.1 myc proto-oncogene protein isoform b



Bos taurus (cattle) MYC NP_001039539.1
Xenopus tropicalis (tropical clawed frog) MGC76254 NP_989390.1
Mus musculus (house mouse) Myc NP_034979.3
Homo sapiens (human) MYC NP_002458.2
Pan troglodytes (chimpanzee) MYC NP_001136266.1
Rattus norvegicus (Norway rat) Myc NP_036735.2
Danio rerio (zebrafish) mycb NP_956466.1
Macaca mulatta (rhesus monkey) MYC NP_001136345.1
Canis lupus familiaris (dog) MYC NP_001003246.2
Gallus gallus (chicken) MYC NP_001026123.1
Danio rerio (zebrafish) myca NP_571487.2


Related articles in PubMed

Opposing macrophage polarization programs show extensive epigenomic and transcriptional cross-talk.
Piccolo V, Curina A, Genua M, Ghisletti S, Simonatto M, Sabò A, Amati B, Ostuni R, Natoli G
Nature immunology18(5)530-540(2017 May)

Craniofacial Ciliopathies Reveal Specific Requirements for GLI Proteins during Development of the Facial Midline.
Chang CF, Chang YT, Millington G, Brugmann SA
PLoS genetics12(11)e1006351(2016 Nov)

Identification of downstream-initiated c-Myc proteins which are dominant-negative inhibitors of transactivation by full-length c-Myc proteins.
Spotts GD, Patel SV, Xiao Q, Hann SR
Molecular and cellular biology17(3)1459-68(1997 Mar)

Nucleotide sequence comparison of normal and translocated murine c-myc genes.
Stanton LW, Fahrlander PD, Tesser PM, Marcu KB
Nature310(5976)423-5(1984 Aug 2-8)

Products of a reciprocal chromosome translocation involving the c-myc gene in a murine plasmacytoma.
Stanton LW, Yang JQ, Eckhardt LA, Harris LJ, Birshtein BK, Marcu KB
Proceedings of the National Academy of Sciences of the United States of America81(3)829-33(1984 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data, including data from studies in cells from knockout mice, suggest that Prmt1 activity was necessary for c-Myc binding to acetyltransferase p300 in myeloid cells; Prmt1 inhibition decreases p300 recruitment to c-Myc target promoters and increased Hdac1 recruitment. [Prmt1, protein arginine N-methyltransferase 1; c-Myc = Proto-Oncogene Proteins c-myc; Hdac1 = histone deacetylase 1]
Title: Arginine methylation regulates c-Myc-dependent transcription by altering promoter recruitment of the acetyltransferase p300.

BRG1/BRM and c-MYC have an antagonistic relationship regulating the expression of cardiac conduction genes that maintain contractility, which is reminiscent of their antagonistic roles as a tumor suppressor and oncogene in cancer.
Title: BRG1 and BRM function antagonistically with c-MYC in adult cardiomyocytes to regulate conduction and contractility.

c-Myc gene expression in tumor tissues of mice treated I.V or IT with MSCs was down-regulated to 28.0% and 16.0% respectively compare to control groups. In conclusion, growth inhibition of breast tumors in mice due to MSC therapy is associated with modulation of c-Myc activation and angiogenesis markers.
Title: Correlation of micro vessel density and c-Myc expression in breast tumor of mice following mesenchymal stem cell therapy.

Early generated B1 B cells with restricted BCRs become chronic lymphocytic leukemia with continued c-Myc and low Bmf expression
Title: Early generated B1 B cells with restricted BCRs become chronic lymphocytic leukemia with continued c-Myc and low Bmf expression.

demonstrate a critical link between Myc/Mycn gene activity and glycolysis in spermatogonial stem cell self-renewal
Title: Myc/Mycn-mediated glycolysis enhances mouse spermatogonial stem cell self-renewal.

The following MYC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OMu22762 NM_001177354.1
Latest version!
Mus musculus myelocytomatosis oncogene (Myc), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OMu22762 NM_001177352.1
Latest version!
Mus musculus myelocytomatosis oncogene (Myc), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OMu22878 NM_010849.4
Latest version!
Mus musculus myelocytomatosis oncogene (Myc), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $272.30
$389.00
OMu23011 NM_001177353.1
Latest version!
Mus musculus myelocytomatosis oncogene (Myc), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $265.30
$379.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OMu22762
Clone ID Related Accession (Same CDS sequence) NM_001177354.1 , NM_001177352.1
Accession Version NM_001177354.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1320bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-07
Organism Mus musculus(House mouse)
Product myc proto-oncogene protein isoform b
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE853266.1, AK133952.1 and BE456204.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. This variant encodes two isoforms due to the use of alternative translation initiation codons. The longer isoform (c) is derived from a non-AUG (CUG) start codon, while a shorter isoform (b, also known as c-Myc 2) is derived from a downstream AUG start codon. The shorter isoform (b) is represented in this RefSeq, and is 15 aa shorter, compared to isoform a. Both variants 1 and 2 can encode isoform b. CCDS Note: This CCDS ID represents the shortest c-Myc isoform, also known as c-Myc 2. Evidence in the literature, including in PMIDs 1628829, 3277717 and 9032273, shows that the transcript can produce multiple isoforms due to the use of alternative translation initiation codons. This isoform initiates translation at a downstream AUG start codon. Alternative translation initiation at an upstream non-AUG (CUG) start codon produces a longer isoform, known as c-Myc 1. The longer isoform is represented by CCDS 27504.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK133952.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:1628829) ##RefSeq-Attributes-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGCCCCTCA ACGTGAACTT CACCAACAGG AACTATGACC TCGACTACGA CTCCGTACAG 
CCCTATTTCA TCTGCGACGA GGAAGAGAAT TTCTATCACC AGCAACAGCA GAGCGAGCTG
CAGCCGCCCG CGCCCAGTGA GGATATCTGG AAGAAATTCG AGCTGCTTCC CACCCCGCCC
CTGTCCCCGA GCCGCCGCTC CGGGCTCTGC TCTCCATCCT ATGTTGCGGT CGCTACGTCC
TTCTCCCCAA GGGAAGACGA TGACGGCGGC GGTGGCAACT TCTCCACCGC CGATCAGCTG
GAGATGATGA CCGAGTTACT TGGAGGAGAC ATGGTGAACC AGAGCTTCAT CTGCGATCCT
GACGACGAGA CCTTCATCAA GAACATCATC ATCCAGGACT GTATGTGGAG CGGTTTCTCA
GCCGCTGCCA AGCTGGTCTC GGAGAAGCTG GCCTCCTACC AGGCTGCGCG CAAAGACAGC
ACCAGCCTGA GCCCCGCCCG CGGGCACAGC GTCTGCTCCA CCTCCAGCCT GTACCTGCAG
GACCTCACCG CCGCCGCGTC CGAGTGCATT GACCCCTCAG TGGTCTTTCC CTACCCGCTC
AACGACAGCA GCTCGCCCAA ATCCTGTACC TCGTCCGATT CCACGGCCTT CTCTCCTTCC
TCGGACTCGC TGCTGTCCTC CGAGTCCTCC CCACGGGCCA GCCCTGAGCC CCTAGTGCTG
CATGAGGAGA CACCGCCCAC CACCAGCAGC GACTCTGAAG AAGAGCAAGA AGATGAGGAA
GAAATTGATG TGGTGTCTGT GGAGAAGAGG CAAACCCCTG CCAAGAGGTC GGAGTCGGGC
TCATCTCCAT CCCGAGGCCA CAGCAAACCT CCGCACAGCC CACTGGTCCT CAAGAGGTGC
CACGTCTCCA CTCACCAGCA CAACTACGCC GCACCCCCCT CCACAAGGAA GGACTATCCA
GCTGCCAAGA GGGCCAAGTT GGACAGTGGC AGGGTCCTGA AGCAGATCAG CAACAACCGC
AAGTGCTCCA GCCCCAGGTC CTCAGACACG GAGGAAAACG ACAAGAGGCG GACACACAAC
GTCTTGGAAC GTCAGAGGAG GAACGAGCTG AAGCGCAGCT TTTTTGCCCT GCGTGACCAG
ATCCCTGAAT TGGAAAACAA CGAAAAGGCC CCCAAGGTAG TGATCCTCAA AAAAGCCACC
GCCTACATCC TGTCCATTCA AGCAGACGAG CACAAGCTCA CCTCTGAAAA GGACTTATTG
AGGAAACGAC GAGAACAGTT GAAACACAAA CTCGAACAGC TTCGAAACTC TGGTGCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001170825.1
CDS624..1943
Misc Feature(1)46
Misc Feature(2)211
Misc Feature(3)576..578
Misc Feature(4)582..584
Misc Feature(5)639..1658
Misc Feature(6)645..647
Misc Feature(7)795..797
Misc Feature(8)807..809
Misc Feature(9)834..836
Misc Feature(10)1053..1055
Misc Feature(11)1068..1070
Misc Feature(12)1095..1097
Misc Feature(13)1107..1109
Misc Feature(14)1446..1448
Misc Feature(15)1500..1502
Misc Feature(16)1572..1574
Misc Feature(17)1590..1592
Misc Feature(18)1608..1610
Misc Feature(19)1677..1856
Misc Feature(20)order(1686..1691,1707..1715,1719..1721,1788..1790,1797..1799)
Misc Feature(21)1710..1712
Misc Feature(22)order(1728..1733,1740..1745,1749..1754,1800..1802,1809..1811,1821..1823,1827..1832,1842..1844,1848..1853)
Misc Feature(23)1734..1736
Misc Feature(24)1845..1934
Misc Feature(25)1860..1925
Exon (1)1..611
Exon (2)612..1380
Exon (3)1381..2396
Translation

Target ORF information:

RefSeq Version NM_001177354.1
Organism Mus musculus(House mouse)
Definition Mus musculus myelocytomatosis oncogene (Myc), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001177354.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGCCCCTCA ACGTGAACTT CACCAACAGG AACTATGACC TCGACTACGA CTCCGTACAG 
CCCTATTTCA TCTGCGACGA GGAAGAGAAT TTCTATCACC AGCAACAGCA GAGCGAGCTG
CAGCCGCCCG CGCCCAGTGA GGATATCTGG AAGAAATTCG AGCTGCTTCC CACCCCGCCC
CTGTCCCCGA GCCGCCGCTC CGGGCTCTGC TCTCCATCCT ATGTTGCGGT CGCTACGTCC
TTCTCCCCAA GGGAAGACGA TGACGGCGGC GGTGGCAACT TCTCCACCGC CGATCAGCTG
GAGATGATGA CCGAGTTACT TGGAGGAGAC ATGGTGAACC AGAGCTTCAT CTGCGATCCT
GACGACGAGA CCTTCATCAA GAACATCATC ATCCAGGACT GTATGTGGAG CGGTTTCTCA
GCCGCTGCCA AGCTGGTCTC GGAGAAGCTG GCCTCCTACC AGGCTGCGCG CAAAGACAGC
ACCAGCCTGA GCCCCGCCCG CGGGCACAGC GTCTGCTCCA CCTCCAGCCT GTACCTGCAG
GACCTCACCG CCGCCGCGTC CGAGTGCATT GACCCCTCAG TGGTCTTTCC CTACCCGCTC
AACGACAGCA GCTCGCCCAA ATCCTGTACC TCGTCCGATT CCACGGCCTT CTCTCCTTCC
TCGGACTCGC TGCTGTCCTC CGAGTCCTCC CCACGGGCCA GCCCTGAGCC CCTAGTGCTG
CATGAGGAGA CACCGCCCAC CACCAGCAGC GACTCTGAAG AAGAGCAAGA AGATGAGGAA
GAAATTGATG TGGTGTCTGT GGAGAAGAGG CAAACCCCTG CCAAGAGGTC GGAGTCGGGC
TCATCTCCAT CCCGAGGCCA CAGCAAACCT CCGCACAGCC CACTGGTCCT CAAGAGGTGC
CACGTCTCCA CTCACCAGCA CAACTACGCC GCACCCCCCT CCACAAGGAA GGACTATCCA
GCTGCCAAGA GGGCCAAGTT GGACAGTGGC AGGGTCCTGA AGCAGATCAG CAACAACCGC
AAGTGCTCCA GCCCCAGGTC CTCAGACACG GAGGAAAACG ACAAGAGGCG GACACACAAC
GTCTTGGAAC GTCAGAGGAG GAACGAGCTG AAGCGCAGCT TTTTTGCCCT GCGTGACCAG
ATCCCTGAAT TGGAAAACAA CGAAAAGGCC CCCAAGGTAG TGATCCTCAA AAAAGCCACC
GCCTACATCC TGTCCATTCA AGCAGACGAG CACAAGCTCA CCTCTGAAAA GGACTTATTG
AGGAAACGAC GAGAACAGTT GAAACACAAA CTCGAACAGC TTCGAAACTC TGGTGCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu22762
Clone ID Related Accession (Same CDS sequence) NM_001177354.1 , NM_001177352.1
Accession Version NM_001177352.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1320bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-07
Organism Mus musculus(House mouse)
Product myc proto-oncogene protein isoform b
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE853266.1, BB858498.1, BC138931.1 and BE456204.1. Transcript Variant: This variant (1) represents the longer transcript and encodes two isoforms due to the use of alternative translation initiation codons. The longer isoform (a, also known as c-Myc 1) is derived from a non-AUG (CUG) start codon, while a shorter isoform (b, also known as c-Myc 2) is derived from a downstream AUG start codon. The shorter isoform (b) is represented in this RefSeq, and is 15 aa shorter, compared to isoform a. Both variants 1 and 2 can encode isoform b. CCDS Note: This CCDS ID represents the shortest c-Myc isoform, also known as c-Myc 2. Evidence in the literature, including in PMIDs 1628829, 3277717 and 9032273, shows that the transcript can produce multiple isoforms due to the use of alternative translation initiation codons. This isoform initiates translation at a downstream AUG start codon. Alternative translation initiation at an upstream non-AUG (CUG) start codon produces a longer isoform, known as c-Myc 1. The longer isoform is represented by CCDS 27504.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK145084.1, X01023.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:1628829) ##RefSeq-Attributes-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGCCCCTCA ACGTGAACTT CACCAACAGG AACTATGACC TCGACTACGA CTCCGTACAG 
CCCTATTTCA TCTGCGACGA GGAAGAGAAT TTCTATCACC AGCAACAGCA GAGCGAGCTG
CAGCCGCCCG CGCCCAGTGA GGATATCTGG AAGAAATTCG AGCTGCTTCC CACCCCGCCC
CTGTCCCCGA GCCGCCGCTC CGGGCTCTGC TCTCCATCCT ATGTTGCGGT CGCTACGTCC
TTCTCCCCAA GGGAAGACGA TGACGGCGGC GGTGGCAACT TCTCCACCGC CGATCAGCTG
GAGATGATGA CCGAGTTACT TGGAGGAGAC ATGGTGAACC AGAGCTTCAT CTGCGATCCT
GACGACGAGA CCTTCATCAA GAACATCATC ATCCAGGACT GTATGTGGAG CGGTTTCTCA
GCCGCTGCCA AGCTGGTCTC GGAGAAGCTG GCCTCCTACC AGGCTGCGCG CAAAGACAGC
ACCAGCCTGA GCCCCGCCCG CGGGCACAGC GTCTGCTCCA CCTCCAGCCT GTACCTGCAG
GACCTCACCG CCGCCGCGTC CGAGTGCATT GACCCCTCAG TGGTCTTTCC CTACCCGCTC
AACGACAGCA GCTCGCCCAA ATCCTGTACC TCGTCCGATT CCACGGCCTT CTCTCCTTCC
TCGGACTCGC TGCTGTCCTC CGAGTCCTCC CCACGGGCCA GCCCTGAGCC CCTAGTGCTG
CATGAGGAGA CACCGCCCAC CACCAGCAGC GACTCTGAAG AAGAGCAAGA AGATGAGGAA
GAAATTGATG TGGTGTCTGT GGAGAAGAGG CAAACCCCTG CCAAGAGGTC GGAGTCGGGC
TCATCTCCAT CCCGAGGCCA CAGCAAACCT CCGCACAGCC CACTGGTCCT CAAGAGGTGC
CACGTCTCCA CTCACCAGCA CAACTACGCC GCACCCCCCT CCACAAGGAA GGACTATCCA
GCTGCCAAGA GGGCCAAGTT GGACAGTGGC AGGGTCCTGA AGCAGATCAG CAACAACCGC
AAGTGCTCCA GCCCCAGGTC CTCAGACACG GAGGAAAACG ACAAGAGGCG GACACACAAC
GTCTTGGAAC GTCAGAGGAG GAACGAGCTG AAGCGCAGCT TTTTTGCCCT GCGTGACCAG
ATCCCTGAAT TGGAAAACAA CGAAAAGGCC CCCAAGGTAG TGATCCTCAA AAAAGCCACC
GCCTACATCC TGTCCATTCA AGCAGACGAG CACAAGCTCA CCTCTGAAAA GGACTTATTG
AGGAAACGAC GAGAACAGTT GAAACACAAA CTCGAACAGC TTCGAAACTC TGGTGCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001170823.1
CDS627..1946
Misc Feature(1)46
Misc Feature(2)211
Misc Feature(3)576..578
Misc Feature(4)582..584
Misc Feature(5)642..1661
Misc Feature(6)648..650
Misc Feature(7)798..800
Misc Feature(8)810..812
Misc Feature(9)837..839
Misc Feature(10)1056..1058
Misc Feature(11)1071..1073
Misc Feature(12)1098..1100
Misc Feature(13)1110..1112
Misc Feature(14)1449..1451
Misc Feature(15)1503..1505
Misc Feature(16)1575..1577
Misc Feature(17)1593..1595
Misc Feature(18)1611..1613
Misc Feature(19)1680..1859
Misc Feature(20)order(1689..1694,1710..1718,1722..1724,1791..1793,1800..1802)
Misc Feature(21)1713..1715
Misc Feature(22)order(1731..1736,1743..1748,1752..1757,1803..1805,1812..1814,1824..1826,1830..1835,1845..1847,1851..1856)
Misc Feature(23)1737..1739
Misc Feature(24)1848..1937
Misc Feature(25)1863..1928
Exon (1)1..611
Exon (2)612..1383
Exon (3)1384..2399
Translation

Target ORF information:

RefSeq Version NM_001177352.1
Organism Mus musculus(House mouse)
Definition Mus musculus myelocytomatosis oncogene (Myc), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001177352.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGCCCCTCA ACGTGAACTT CACCAACAGG AACTATGACC TCGACTACGA CTCCGTACAG 
CCCTATTTCA TCTGCGACGA GGAAGAGAAT TTCTATCACC AGCAACAGCA GAGCGAGCTG
CAGCCGCCCG CGCCCAGTGA GGATATCTGG AAGAAATTCG AGCTGCTTCC CACCCCGCCC
CTGTCCCCGA GCCGCCGCTC CGGGCTCTGC TCTCCATCCT ATGTTGCGGT CGCTACGTCC
TTCTCCCCAA GGGAAGACGA TGACGGCGGC GGTGGCAACT TCTCCACCGC CGATCAGCTG
GAGATGATGA CCGAGTTACT TGGAGGAGAC ATGGTGAACC AGAGCTTCAT CTGCGATCCT
GACGACGAGA CCTTCATCAA GAACATCATC ATCCAGGACT GTATGTGGAG CGGTTTCTCA
GCCGCTGCCA AGCTGGTCTC GGAGAAGCTG GCCTCCTACC AGGCTGCGCG CAAAGACAGC
ACCAGCCTGA GCCCCGCCCG CGGGCACAGC GTCTGCTCCA CCTCCAGCCT GTACCTGCAG
GACCTCACCG CCGCCGCGTC CGAGTGCATT GACCCCTCAG TGGTCTTTCC CTACCCGCTC
AACGACAGCA GCTCGCCCAA ATCCTGTACC TCGTCCGATT CCACGGCCTT CTCTCCTTCC
TCGGACTCGC TGCTGTCCTC CGAGTCCTCC CCACGGGCCA GCCCTGAGCC CCTAGTGCTG
CATGAGGAGA CACCGCCCAC CACCAGCAGC GACTCTGAAG AAGAGCAAGA AGATGAGGAA
GAAATTGATG TGGTGTCTGT GGAGAAGAGG CAAACCCCTG CCAAGAGGTC GGAGTCGGGC
TCATCTCCAT CCCGAGGCCA CAGCAAACCT CCGCACAGCC CACTGGTCCT CAAGAGGTGC
CACGTCTCCA CTCACCAGCA CAACTACGCC GCACCCCCCT CCACAAGGAA GGACTATCCA
GCTGCCAAGA GGGCCAAGTT GGACAGTGGC AGGGTCCTGA AGCAGATCAG CAACAACCGC
AAGTGCTCCA GCCCCAGGTC CTCAGACACG GAGGAAAACG ACAAGAGGCG GACACACAAC
GTCTTGGAAC GTCAGAGGAG GAACGAGCTG AAGCGCAGCT TTTTTGCCCT GCGTGACCAG
ATCCCTGAAT TGGAAAACAA CGAAAAGGCC CCCAAGGTAG TGATCCTCAA AAAAGCCACC
GCCTACATCC TGTCCATTCA AGCAGACGAG CACAAGCTCA CCTCTGAAAA GGACTTATTG
AGGAAACGAC GAGAACAGTT GAAACACAAA CTCGAACAGC TTCGAAACTC TGGTGCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu22878
Clone ID Related Accession (Same CDS sequence) NM_010849.4
Accession Version NM_010849.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1365bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-07
Organism Mus musculus(House mouse)
Product myc proto-oncogene protein isoform a
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE853266.1, BB858498.1, BC138931.1 and BE456204.1. On May 25, 2006 this sequence version replaced NM_010849.3. Transcript Variant: This variant (1) represents the longer transcript and encodes two isoforms due to the use of alternative translation initiation codons. The longer isoform (a, also known as c-Myc 1) is derived from a non-AUG (CUG) start codon, while a shorter isoform (b, also known as c-Myc 2) is derived from a downstream AUG start codon. The longer isoform (a) is represented in this RefSeq. CCDS Note: This CCDS ID represents the longest c-Myc isoform, also known as c-Myc 1. Evidence in the literature, including in PMIDs 1628829, 3277717 and 9032273, shows that the transcript can produce multiple isoforms due to the use of alternative translation initiation codons. This transcript variant is supported by several transcripts, including the long mRNAs X01023.1, AK145084.1 and BC138932.1. This isoform initiates translation at a non-AUG (CUG) start codon that is well-conserved. Alternative translation initiation at a downstream AUG start codon also produces a shorter isoform, known as c-Myc 2. The shorter isoform is represented by CCDS 49615.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK145084.1, X01023.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 1628829 ##RefSeq-Attributes-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
CTGGATTTCC TTTGGGCGTT GGAAACCCCG CAGACAGCCA CGACGATGCC CCTCAACGTG 
AACTTCACCA ACAGGAACTA TGACCTCGAC TACGACTCCG TACAGCCCTA TTTCATCTGC
GACGAGGAAG AGAATTTCTA TCACCAGCAA CAGCAGAGCG AGCTGCAGCC GCCCGCGCCC
AGTGAGGATA TCTGGAAGAA ATTCGAGCTG CTTCCCACCC CGCCCCTGTC CCCGAGCCGC
CGCTCCGGGC TCTGCTCTCC ATCCTATGTT GCGGTCGCTA CGTCCTTCTC CCCAAGGGAA
GACGATGACG GCGGCGGTGG CAACTTCTCC ACCGCCGATC AGCTGGAGAT GATGACCGAG
TTACTTGGAG GAGACATGGT GAACCAGAGC TTCATCTGCG ATCCTGACGA CGAGACCTTC
ATCAAGAACA TCATCATCCA GGACTGTATG TGGAGCGGTT TCTCAGCCGC TGCCAAGCTG
GTCTCGGAGA AGCTGGCCTC CTACCAGGCT GCGCGCAAAG ACAGCACCAG CCTGAGCCCC
GCCCGCGGGC ACAGCGTCTG CTCCACCTCC AGCCTGTACC TGCAGGACCT CACCGCCGCC
GCGTCCGAGT GCATTGACCC CTCAGTGGTC TTTCCCTACC CGCTCAACGA CAGCAGCTCG
CCCAAATCCT GTACCTCGTC CGATTCCACG GCCTTCTCTC CTTCCTCGGA CTCGCTGCTG
TCCTCCGAGT CCTCCCCACG GGCCAGCCCT GAGCCCCTAG TGCTGCATGA GGAGACACCG
CCCACCACCA GCAGCGACTC TGAAGAAGAG CAAGAAGATG AGGAAGAAAT TGATGTGGTG
TCTGTGGAGA AGAGGCAAAC CCCTGCCAAG AGGTCGGAGT CGGGCTCATC TCCATCCCGA
GGCCACAGCA AACCTCCGCA CAGCCCACTG GTCCTCAAGA GGTGCCACGT CTCCACTCAC
CAGCACAACT ACGCCGCACC CCCCTCCACA AGGAAGGACT ATCCAGCTGC CAAGAGGGCC
AAGTTGGACA GTGGCAGGGT CCTGAAGCAG ATCAGCAACA ACCGCAAGTG CTCCAGCCCC
AGGTCCTCAG ACACGGAGGA AAACGACAAG AGGCGGACAC ACAACGTCTT GGAACGTCAG
AGGAGGAACG AGCTGAAGCG CAGCTTTTTT GCCCTGCGTG ACCAGATCCC TGAATTGGAA
AACAACGAAA AGGCCCCCAA GGTAGTGATC CTCAAAAAAG CCACCGCCTA CATCCTGTCC
ATTCAAGCAG ACGAGCACAA GCTCACCTCT GAAAAGGACT TATTGAGGAA ACGACGAGAA
CAGTTGAAAC ACAAACTCGA ACAGCTTCGA AACTCTGGTG CATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_034979.3
CDS582..1946
Misc Feature(1)46
Misc Feature(2)211
Misc Feature(3)576..578
Misc Feature(4)627..629
Misc Feature(5)642..1661
Misc Feature(6)1680..1859
Misc Feature(7)order(1689..1694,1710..1718,1722..1724,1791..1793,1800..1802)
Misc Feature(8)1713..1715
Misc Feature(9)order(1731..1736,1743..1748,1752..1757,1803..1805,1812..1814,1824..1826,1830..1835,1845..1847,1851..1856)
Misc Feature(10)1848..1937
Exon (1)1..611
Exon (2)612..1383
Exon (3)1384..2399
Translation

Target ORF information:

RefSeq Version NM_010849.4
Organism Mus musculus(House mouse)
Definition Mus musculus myelocytomatosis oncogene (Myc), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_010849.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
CTGGATTTCC TTTGGGCGTT GGAAACCCCG CAGACAGCCA CGACGATGCC CCTCAACGTG 
AACTTCACCA ACAGGAACTA TGACCTCGAC TACGACTCCG TACAGCCCTA TTTCATCTGC
GACGAGGAAG AGAATTTCTA TCACCAGCAA CAGCAGAGCG AGCTGCAGCC GCCCGCGCCC
AGTGAGGATA TCTGGAAGAA ATTCGAGCTG CTTCCCACCC CGCCCCTGTC CCCGAGCCGC
CGCTCCGGGC TCTGCTCTCC ATCCTATGTT GCGGTCGCTA CGTCCTTCTC CCCAAGGGAA
GACGATGACG GCGGCGGTGG CAACTTCTCC ACCGCCGATC AGCTGGAGAT GATGACCGAG
TTACTTGGAG GAGACATGGT GAACCAGAGC TTCATCTGCG ATCCTGACGA CGAGACCTTC
ATCAAGAACA TCATCATCCA GGACTGTATG TGGAGCGGTT TCTCAGCCGC TGCCAAGCTG
GTCTCGGAGA AGCTGGCCTC CTACCAGGCT GCGCGCAAAG ACAGCACCAG CCTGAGCCCC
GCCCGCGGGC ACAGCGTCTG CTCCACCTCC AGCCTGTACC TGCAGGACCT CACCGCCGCC
GCGTCCGAGT GCATTGACCC CTCAGTGGTC TTTCCCTACC CGCTCAACGA CAGCAGCTCG
CCCAAATCCT GTACCTCGTC CGATTCCACG GCCTTCTCTC CTTCCTCGGA CTCGCTGCTG
TCCTCCGAGT CCTCCCCACG GGCCAGCCCT GAGCCCCTAG TGCTGCATGA GGAGACACCG
CCCACCACCA GCAGCGACTC TGAAGAAGAG CAAGAAGATG AGGAAGAAAT TGATGTGGTG
TCTGTGGAGA AGAGGCAAAC CCCTGCCAAG AGGTCGGAGT CGGGCTCATC TCCATCCCGA
GGCCACAGCA AACCTCCGCA CAGCCCACTG GTCCTCAAGA GGTGCCACGT CTCCACTCAC
CAGCACAACT ACGCCGCACC CCCCTCCACA AGGAAGGACT ATCCAGCTGC CAAGAGGGCC
AAGTTGGACA GTGGCAGGGT CCTGAAGCAG ATCAGCAACA ACCGCAAGTG CTCCAGCCCC
AGGTCCTCAG ACACGGAGGA AAACGACAAG AGGCGGACAC ACAACGTCTT GGAACGTCAG
AGGAGGAACG AGCTGAAGCG CAGCTTTTTT GCCCTGCGTG ACCAGATCCC TGAATTGGAA
AACAACGAAA AGGCCCCCAA GGTAGTGATC CTCAAAAAAG CCACCGCCTA CATCCTGTCC
ATTCAAGCAG ACGAGCACAA GCTCACCTCT GAAAAGGACT TATTGAGGAA ACGACGAGAA
CAGTTGAAAC ACAAACTCGA ACAGCTTCGA AACTCTGGTG CATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu23011
Clone ID Related Accession (Same CDS sequence) NM_001177353.1
Accession Version NM_001177353.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1362bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-07
Organism Mus musculus(House mouse)
Product myc proto-oncogene protein isoform c
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE853266.1, AK133952.1 and BE456204.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. This variant encodes two isoforms due to the use of alternative translation initiation codons. The longer isoform (c) is derived from a non-AUG (CUG) start codon, while a shorter isoform (b, also known as c-Myc 2) is derived from a downstream AUG start codon. The longer isoform (c) is represented in this RefSeq, and is 1 aa shorter, compared to isoform a. CCDS Note: This CCDS ID represents a c-Myc isoform that initiates translation at a well-conserved non-AUG (CUG) start codon. Evidence in the literature, including in PMIDs 1628829, 3277717 and 9032273, shows that the transcript can produce multiple isoforms due to the use of alternative translation initiation codons. This transcript variant is supported by the long mRNA AK133952.1 and by several ESTs. Alternative translation initiation at a downstream AUG start codon also produces a shorter isoform, known as c-Myc 2. The shorter isoform is represented by CCDS 49615.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK133952.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 1628829 ##RefSeq-Attributes-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
CTGGATTTCC TTTGGGCGTT GGAAACCCCG ACAGCCACGA CGATGCCCCT CAACGTGAAC 
TTCACCAACA GGAACTATGA CCTCGACTAC GACTCCGTAC AGCCCTATTT CATCTGCGAC
GAGGAAGAGA ATTTCTATCA CCAGCAACAG CAGAGCGAGC TGCAGCCGCC CGCGCCCAGT
GAGGATATCT GGAAGAAATT CGAGCTGCTT CCCACCCCGC CCCTGTCCCC GAGCCGCCGC
TCCGGGCTCT GCTCTCCATC CTATGTTGCG GTCGCTACGT CCTTCTCCCC AAGGGAAGAC
GATGACGGCG GCGGTGGCAA CTTCTCCACC GCCGATCAGC TGGAGATGAT GACCGAGTTA
CTTGGAGGAG ACATGGTGAA CCAGAGCTTC ATCTGCGATC CTGACGACGA GACCTTCATC
AAGAACATCA TCATCCAGGA CTGTATGTGG AGCGGTTTCT CAGCCGCTGC CAAGCTGGTC
TCGGAGAAGC TGGCCTCCTA CCAGGCTGCG CGCAAAGACA GCACCAGCCT GAGCCCCGCC
CGCGGGCACA GCGTCTGCTC CACCTCCAGC CTGTACCTGC AGGACCTCAC CGCCGCCGCG
TCCGAGTGCA TTGACCCCTC AGTGGTCTTT CCCTACCCGC TCAACGACAG CAGCTCGCCC
AAATCCTGTA CCTCGTCCGA TTCCACGGCC TTCTCTCCTT CCTCGGACTC GCTGCTGTCC
TCCGAGTCCT CCCCACGGGC CAGCCCTGAG CCCCTAGTGC TGCATGAGGA GACACCGCCC
ACCACCAGCA GCGACTCTGA AGAAGAGCAA GAAGATGAGG AAGAAATTGA TGTGGTGTCT
GTGGAGAAGA GGCAAACCCC TGCCAAGAGG TCGGAGTCGG GCTCATCTCC ATCCCGAGGC
CACAGCAAAC CTCCGCACAG CCCACTGGTC CTCAAGAGGT GCCACGTCTC CACTCACCAG
CACAACTACG CCGCACCCCC CTCCACAAGG AAGGACTATC CAGCTGCCAA GAGGGCCAAG
TTGGACAGTG GCAGGGTCCT GAAGCAGATC AGCAACAACC GCAAGTGCTC CAGCCCCAGG
TCCTCAGACA CGGAGGAAAA CGACAAGAGG CGGACACACA ACGTCTTGGA ACGTCAGAGG
AGGAACGAGC TGAAGCGCAG CTTTTTTGCC CTGCGTGACC AGATCCCTGA ATTGGAAAAC
AACGAAAAGG CCCCCAAGGT AGTGATCCTC AAAAAAGCCA CCGCCTACAT CCTGTCCATT
CAAGCAGACG AGCACAAGCT CACCTCTGAA AAGGACTTAT TGAGGAAACG ACGAGAACAG
TTGAAACACA AACTCGAACA GCTTCGAAAC TCTGGTGCAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001170824.1
CDS582..1943
Misc Feature(1)46
Misc Feature(2)211
Misc Feature(3)576..578
Misc Feature(4)624..626
Misc Feature(5)639..1658
Misc Feature(6)1677..1856
Misc Feature(7)order(1686..1691,1707..1715,1719..1721,1788..1790,1797..1799)
Misc Feature(8)1710..1712
Misc Feature(9)order(1728..1733,1740..1745,1749..1754,1800..1802,1809..1811,1821..1823,1827..1832,1842..1844,1848..1853)
Misc Feature(10)1845..1934
Exon (1)1..611
Exon (2)612..1380
Exon (3)1381..2396
Translation

Target ORF information:

RefSeq Version NM_001177353.1
Organism Mus musculus(House mouse)
Definition Mus musculus myelocytomatosis oncogene (Myc), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001177353.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
CTGGATTTCC TTTGGGCGTT GGAAACCCCG ACAGCCACGA CGATGCCCCT CAACGTGAAC 
TTCACCAACA GGAACTATGA CCTCGACTAC GACTCCGTAC AGCCCTATTT CATCTGCGAC
GAGGAAGAGA ATTTCTATCA CCAGCAACAG CAGAGCGAGC TGCAGCCGCC CGCGCCCAGT
GAGGATATCT GGAAGAAATT CGAGCTGCTT CCCACCCCGC CCCTGTCCCC GAGCCGCCGC
TCCGGGCTCT GCTCTCCATC CTATGTTGCG GTCGCTACGT CCTTCTCCCC AAGGGAAGAC
GATGACGGCG GCGGTGGCAA CTTCTCCACC GCCGATCAGC TGGAGATGAT GACCGAGTTA
CTTGGAGGAG ACATGGTGAA CCAGAGCTTC ATCTGCGATC CTGACGACGA GACCTTCATC
AAGAACATCA TCATCCAGGA CTGTATGTGG AGCGGTTTCT CAGCCGCTGC CAAGCTGGTC
TCGGAGAAGC TGGCCTCCTA CCAGGCTGCG CGCAAAGACA GCACCAGCCT GAGCCCCGCC
CGCGGGCACA GCGTCTGCTC CACCTCCAGC CTGTACCTGC AGGACCTCAC CGCCGCCGCG
TCCGAGTGCA TTGACCCCTC AGTGGTCTTT CCCTACCCGC TCAACGACAG CAGCTCGCCC
AAATCCTGTA CCTCGTCCGA TTCCACGGCC TTCTCTCCTT CCTCGGACTC GCTGCTGTCC
TCCGAGTCCT CCCCACGGGC CAGCCCTGAG CCCCTAGTGC TGCATGAGGA GACACCGCCC
ACCACCAGCA GCGACTCTGA AGAAGAGCAA GAAGATGAGG AAGAAATTGA TGTGGTGTCT
GTGGAGAAGA GGCAAACCCC TGCCAAGAGG TCGGAGTCGG GCTCATCTCC ATCCCGAGGC
CACAGCAAAC CTCCGCACAG CCCACTGGTC CTCAAGAGGT GCCACGTCTC CACTCACCAG
CACAACTACG CCGCACCCCC CTCCACAAGG AAGGACTATC CAGCTGCCAA GAGGGCCAAG
TTGGACAGTG GCAGGGTCCT GAAGCAGATC AGCAACAACC GCAAGTGCTC CAGCCCCAGG
TCCTCAGACA CGGAGGAAAA CGACAAGAGG CGGACACACA ACGTCTTGGA ACGTCAGAGG
AGGAACGAGC TGAAGCGCAG CTTTTTTGCC CTGCGTGACC AGATCCCTGA ATTGGAAAAC
AACGAAAAGG CCCCCAAGGT AGTGATCCTC AAAAAAGCCA CCGCCTACAT CCTGTCCATT
CAAGCAGACG AGCACAAGCT CACCTCTGAA AAGGACTTAT TGAGGAAACG ACGAGAACAG
TTGAAACACA AACTCGAACA GCTTCGAAAC TCTGGTGCAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Opposing macrophage polarization programs show extensive epigenomic and transcriptional cross-talk.
Nature immunology18(5)530-540(2017 May)
Piccolo V,Curina A,Genua M,Ghisletti S,Simonatto M,Sabò A,Amati B,Ostuni R,Natoli G


book

Craniofacial Ciliopathies Reveal Specific Requirements for GLI Proteins during Development of the Facial Midline.
PLoS genetics12(11)e1006351(2016 Nov)
Chang CF,Chang YT,Millington G,Brugmann SA


book

Identification of downstream-initiated c-Myc proteins which are dominant-negative inhibitors of transactivation by full-length c-Myc proteins.
Molecular and cellular biology17(3)1459-68(1997 Mar)
Spotts GD,Patel SV,Xiao Q,Hann SR


book

Nucleotide sequence comparison of normal and translocated murine c-myc genes.
Nature310(5976)423-5(1984 Aug 2-8)
Stanton LW,Fahrlander PD,Tesser PM,Marcu KB


book

Products of a reciprocal chromosome translocation involving the c-myc gene in a murine plasmacytoma.
Proceedings of the National Academy of Sciences of the United States of America81(3)829-33(1984 Feb)
Stanton LW,Yang JQ,Eckhardt LA,Harris LJ,Birshtein BK,Marcu KB


 
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