The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma, in human. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini, in human and mouse. Under conditions of stress, such as high cell densities and methionine deprivation, there is a specific and dramatic increase in the synthesis of the non-AUG initiated protein, suggesting its importance in times of adversity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010].
The following Myc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Myc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE853266.1, BB858498.1, BC138931.1 and BE456204.1. On May 25, 2006 this sequence version replaced NM_010849.3.
Transcript Variant: This variant (1) represents the longer transcript and encodes two isoforms due to the use of alternative translation initiation codons. The longer isoform (a, also known as c-Myc 1) is derived from a non-AUG (CUG) start codon, while a shorter isoform (b, also known as c-Myc 2) is derived from a downstream AUG start codon. The longer isoform (a) is represented in this RefSeq.
CCDS Note: This CCDS ID represents the longest c-Myc isoform, also known as c-Myc 1. Evidence in the literature, including in PMIDs 1628829, 3277717 and 9032273, shows that the transcript can produce multiple isoforms due to the use of alternative translation initiation codons. This transcript variant is supported by several transcripts, including the long mRNAs X01023.1, AK145084.1 and BC138932.1. This isoform initiates translation at a non-AUG (CUG) start codon that is well-conserved. Alternative translation initiation at a downstream AUG start codon also produces a shorter isoform, known as c-Myc 2. The shorter isoform is represented by CCDS 49615.1.
Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.
##Evidence-Data-START##
Transcript exon combination :: AK145084.1, X01023.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348]
##Evidence-Data-END##
##RefSeq-Attributes-START##
non-AUG initiation codon :: PMID: 1628829
##RefSeq-Attributes-END##
COMPLETENESS: full length.
Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE853266.1, AK133952.1 and BE456204.1.
Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. This variant encodes two isoforms due to the use of alternative translation initiation codons. The longer isoform (c) is derived from a non-AUG (CUG) start codon, while a shorter isoform (b, also known as c-Myc 2) is derived from a downstream AUG start codon. The longer isoform (c) is represented in this RefSeq, and is 1 aa shorter, compared to isoform a.
CCDS Note: This CCDS ID represents a c-Myc isoform that initiates translation at a well-conserved non-AUG (CUG) start codon. Evidence in the literature, including in PMIDs 1628829, 3277717 and 9032273, shows that the transcript can produce multiple isoforms due to the use of alternative translation initiation codons. This transcript variant is supported by the long mRNA AK133952.1 and by several ESTs. Alternative translation initiation at a downstream AUG start codon also produces a shorter isoform, known as c-Myc 2. The shorter isoform is represented by CCDS 49615.1.
Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.
##Evidence-Data-START##
Transcript exon combination :: AK133952.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348]
##Evidence-Data-END##
##RefSeq-Attributes-START##
non-AUG initiation codon :: PMID: 1628829
##RefSeq-Attributes-END##
COMPLETENESS: full length.
Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE853266.1, AK133952.1 and BE456204.1.
Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. This variant encodes two isoforms due to the use of alternative translation initiation codons. The longer isoform (c) is derived from a non-AUG (CUG) start codon, while a shorter isoform (b, also known as c-Myc 2) is derived from a downstream AUG start codon. The shorter isoform (b) is represented in this RefSeq, and is 15 aa shorter, compared to isoform a. Both variants 1 and 2 can encode isoform b.
CCDS Note: This CCDS ID represents the shortest c-Myc isoform, also known as c-Myc 2. Evidence in the literature, including in PMIDs 1628829, 3277717 and 9032273, shows that the transcript can produce multiple isoforms due to the use of alternative translation initiation codons. This isoform initiates translation at a downstream AUG start codon. Alternative translation initiation at an upstream non-AUG (CUG) start codon produces a longer isoform, known as c-Myc 1. The longer isoform is represented by CCDS 27504.1.
Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.
##Evidence-Data-START##
Transcript exon combination :: AK133952.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348]
##Evidence-Data-END##
##RefSeq-Attributes-START##
CDS uses downstream in-frame AUG :: experimental evidence (PMID:1628829)
##RefSeq-Attributes-END##
COMPLETENESS: full length.
Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE853266.1, BB858498.1, BC138931.1 and BE456204.1.
Transcript Variant: This variant (1) represents the longer transcript and encodes two isoforms due to the use of alternative translation initiation codons. The longer isoform (a, also known as c-Myc 1) is derived from a non-AUG (CUG) start codon, while a shorter isoform (b, also known as c-Myc 2) is derived from a downstream AUG start codon. The shorter isoform (b) is represented in this RefSeq, and is 15 aa shorter, compared to isoform a. Both variants 1 and 2 can encode isoform b.
CCDS Note: This CCDS ID represents the shortest c-Myc isoform, also known as c-Myc 2. Evidence in the literature, including in PMIDs 1628829, 3277717 and 9032273, shows that the transcript can produce multiple isoforms due to the use of alternative translation initiation codons. This isoform initiates translation at a downstream AUG start codon. Alternative translation initiation at an upstream non-AUG (CUG) start codon produces a longer isoform, known as c-Myc 1. The longer isoform is represented by CCDS 27504.1.
Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.
##Evidence-Data-START##
Transcript exon combination :: AK145084.1, X01023.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348]
##Evidence-Data-END##
##RefSeq-Attributes-START##
CDS uses downstream in-frame AUG :: experimental evidence (PMID:1628829)
##RefSeq-Attributes-END##
COMPLETENESS: full length.
User Manual
NM_010849.4
