Npc1 cDNA ORF clone, Mus musculus(house mouse)
| Gene Symbol | Npc1 |
| Entrez Gene ID | 18145 |
| Full Name | NPC intracellular cholesterol transporter 1 |
| Synonyms | A430089E03Rik,C85354,D18Ertd139e,D18Ertd723e,lcsd,nmf164,spm |
| General protein information |
|
| Gene Type | protein-coding |
| Organism | Mus musculus(house mouse) |
| Genome |
mRNA and Protein(s)
| mRNA | Protein | Name |
|---|---|---|
| NM_008720.2 | NP_032746.2 | Niemann-Pick C1 protein precursor |
| XM_017317849.1 | XP_017173338.1 | Niemann-Pick C1 protein isoform X1 |
| Canis lupus familiaris (dog) | NPC1 | NP_001003107.1 |
| Bos taurus (cattle) | NPC1 | NP_777183.1 |
| Kluyveromyces lactis | KLLA0F27137g | XP_456287.1 |
| Magnaporthe oryzae (rice blast fungus) | MGG_00819 | XP_003718164.1 |
| Mus musculus (house mouse) | Npc1 | NP_032746.2 |
| Drosophila melanogaster (fruit fly) | Npc1a | NP_001188769.1 |
| Homo sapiens (human) | NPC1 | NP_000262.2 |
| Pan troglodytes (chimpanzee) | NPC1 | XP_001155285.1 |
| NPC1 | XP_002800934.1 | |
| Anopheles gambiae (African malaria mosquito) | AgaP_AGAP008137 | XP_317322.4 |
| Rattus norvegicus (Norway rat) | Npc1 | NP_705888.2 |
| Danio rerio (zebrafish) | npc1 | NP_001230804.1 |
| Saccharomyces cerevisiae (baker's yeast) | NCR1 | NP_015319.1 |
| Eremothecium gossypii | AGOS_AGL008W | NP_986658.1 |
| Neurospora crassa | NCU04090 | XP_956086.1 |
| Arabidopsis thaliana (thale cress) | AT1G42470 | NP_174975.2 |
| Gallus gallus (chicken) | NPC1 | XP_419162.3 |
| Arabidopsis thaliana (thale cress) | AT4G38350 | NP_001190953.1 |
| Xenopus tropicalis (tropical clawed frog) | npc1 | XP_004915269.1 |
Related articles in PubMed
The Niemann-Pick C1 gene interacts with a high-fat diet to promote weight gain through differential regulation of central energy metabolism pathways.
Castillo JJ, Jelinek D, Wei H, Gannon NP, Vaughan RA, Horwood LJ, Meaney FJ, Garcia-Smith R, Trujillo KA, Heidenreich RA, Meyre D, Orlando RA, LeBoeuf RC, Garver WS
American journal of physiology. Endocrinology and metabolism313(2)E183-E194(2017 Aug)
Shortened primary cilium length and dysregulated Sonic hedgehog signaling in Niemann-Pick C1 disease.
Canterini S, Dragotto J, Dardis A, Zampieri S, De Stefano ME, Mangia F, Erickson RP, Fiorenza MT
Human molecular genetics26(12)2277-2289(2017 06)
Rare Loss-of-Function Variants in
Liu R, Zou Y, Hong J, Cao M, Cui B, Zhang H, Chen M, Shi J, Ning T, Zhao S, Liu W, Xiong H, Wei C, Qiu Z, Gu W, Zhang Y, Li W, Miao L, Sun Y, Yang M, Wang R, Ma Q, Xu M, Xu Y, Wang T, Chan KK, Zuo X, Chen H, Qi L, Lai S, Duan S, Song B, Bi Y, Liu S, Wang W, Ning G, Wang J
Diabetes66(4)935-947(2017 04)
AAV9-NPC1 significantly ameliorates Purkinje cell death and behavioral abnormalities in mouse NPC disease.
Xie C, Gong XM, Luo J, Li BL, Song BL
Journal of lipid research58(3)512-518(2017 Mar)
Normalization of Hepatic Homeostasis in the
Munkacsi AB, Hammond N, Schneider RT, Senanayake DS, Higaki K, Lagutin K, Bloor SJ, Ory DS, Maue RA, Chen FW, Hernandez-Ono A, Dahlson N, Repa JJ, Ginsberg HN, Ioannou YA, Sturley SL
The Journal of biological chemistry292(11)4395-4410(2017 03)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
Npc1 gene interacts with a high fat diet to promote weight gain through differential regulation of central energy metabolism pathways.
Title: The Niemann-Pick C1 gene interacts with a high-fat diet to promote weight gain through differential regulation of central energy metabolism pathways.
the spleen is significantly enlarged in Npc1(-/-) mice.
Title: Telocytes: a potential defender in the spleen of Npc1 mutant mice.
High-resolution respirometry analyses revealed that GSH-EE improved oxidative phosphorylation, coupled respiration and maximal electron transfer in cerebellum of Npc1(-/-) mice
Title: Mitochondrial GSH replenishment as a potential therapeutic approach for Niemann Pick type C disease.
Male NPC1+/- mice had increased fat storage while eating a high-fat diet.
Title: Rare Loss-of-Function Variants in
AAV9-mediated NPC1 delivery significantly promoted Purkinje cell survival, restored locomotor activity and coordination, and increased the lifespan of NPC1(-/-) mice. Our work suggests that AAV-based gene therapy is a promising means to treat NPC disease.
Title: AAV9-NPC1 significantly ameliorates Purkinje cell death and behavioral abnormalities in mouse NPC disease.
The following Npc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Npc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu79770 | |
| Clone ID Related Accession (Same CDS sequence) | XM_017317849.1 | |
| Accession Version | XM_017317849.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3831bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ® Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-06-22 | |
| Organism | Mus musculus(house mouse) | |
| Product | Niemann-Pick C1 protein isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGGTGCGC ACCACCCGGC CCTCGGCCTG CTGCTGCTGC TGCTGTGCCC TGCGCAGGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_017173338.1 |
| CDS | 196..4026 |
| Translation |
Target ORF information:
Target ORF information:
|
 XM_017317849.1 |
ORF Insert Sequence:
1 | ATGGGTGCGC ACCACCCGGC CCTCGGCCTG CTGCTGCTGC TGCTGTGCCC TGCGCAGGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu21708 | |
| Clone ID Related Accession (Same CDS sequence) | NM_008720.2 | |
| Accession Version | NM_008720.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3834bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ® Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-12-06 | |
| Organism | Mus musculus(house mouse) | |
| Product | Niemann-Pick C1 protein precursor | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC052437.1, CF585180.1 and AK003935.1. On Mar 7, 2006 this sequence version replaced NM_008720.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC052437.1, AF003348.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## | |
1 | ATGGGTGCGC ACCACCCGGC CCTCGGCCTG CTGCTGCTGC TGCTGTGCCC TGCGCAGGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_032746.2 |
| CDS | 150..3983 |
| Misc Feature(1) | 216..950 |
| Misc Feature(2) | 222..3902 |
| Misc Feature(3) | 471..473 |
| Misc Feature(4) | 672..764 |
| Misc Feature(5) | 957..1019 |
| Misc Feature(6) | 1200..1262 |
| Misc Feature(7) | 2013..2075 |
| Misc Feature(8) | 2094..2555 |
| Misc Feature(9) | 2109..2171 |
| Misc Feature(10) | 2199..2261 |
| Misc Feature(11) | 2340..2402 |
| Misc Feature(12) | 2427..2489 |
| Misc Feature(13) | 2646..2708 |
| Misc Feature(14) | 3378..3431 |
| Misc Feature(15) | 3441..3503 |
| Misc Feature(16) | 3519..3581 |
| Misc Feature(17) | 3585..3647 |
| Misc Feature(18) | 3732..3794 |
| Misc Feature(19) | 3828..3890 |
| Misc Feature(20) | 3969..3980 |
| Exon (1) | 1..206 |
| Exon (2) | 207..329 |
| Exon (3) | 330..436 |
| Exon (4) | 437..612 |
| Exon (5) | 613..780 |
| Exon (6) | 781..1030 |
| Exon (7) | 1031..1104 |
| Exon (8) | 1105..1475 |
| Exon (9) | 1476..1702 |
| Exon (10) | 1703..1803 |
| Exon (11) | 1804..1906 |
| Exon (12) | 1907..2096 |
| Exon (13) | 2097..2279 |
| Exon (14) | 2280..2394 |
| Exon (15) | 2395..2522 |
| Exon (16) | 2523..2663 |
| Exon (17) | 2664..2753 |
| Exon (18) | 2754..2944 |
| Exon (19) | 2945..3060 |
| Exon (20) | 3061..3190 |
| Exon (21) | 3191..3391 |
| Exon (22) | 3392..3623 |
| Exon (23) | 3624..3737 |
| Exon (24) | 3738..3900 |
| Exon (25) | 3901..5209 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_008720.2 |
ORF Insert Sequence:
1 | ATGGGTGCGC ACCACCCGGC CCTCGGCCTG CTGCTGCTGC TGCTGTGCCC TGCGCAGGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
 |
The Niemann-Pick C1 gene interacts with a high-fat diet to promote weight gain through differential regulation of central energy metabolism pathways. |
|
Shortened primary cilium length and dysregulated Sonic hedgehog signaling in Niemann-Pick C1 disease. |
|
Rare Loss-of-Function Variants in |
|
AAV9-NPC1 significantly ameliorates Purkinje cell death and behavioral abnormalities in mouse NPC disease. |
|
Normalization of Hepatic Homeostasis in the |