The following Npc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Npc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID |
OMu79770 |
| Clone ID Related Accession (Same CDS sequence) |
XM_017317849.1
|
| Accession Version |
XM_017317849.1 Latest version! |
Documents for ORF clone product in default vector |
| Sequence Information |
ORF Nucleotide Sequence (Length: 3831bp)
Protein sequence
SNP |
| Vector |
pcDNA3.1+/C-(K)DYK or customized vector |
 User Manual |
| Clone information |
Clone Map |
MSDS |
| Tag on pcDNA3.1+/C-(K)DYK |
C terminal DYKDDDDK tags |
| ORF Insert Method |
CloneEZ™ Seamless cloning technology |
| Insert Structure |
linear |
| Update Date |
2016-06-21 |
| Organism |
Mus musculus(house mouse) |
| Product |
Niemann-Pick C1 protein isoform X1 |
| Comment |
Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process
##Genome-Annotation-Data-START##
Annotation Provider :: NCBI
Annotation Status :: Full annotation
Annotation Version :: Mus musculus Annotation Release 106
Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline
Annotation Software Version :: 7.1
Annotation Method :: Best-placed RefSeq; Gnomon
Features Annotated :: Gene; mRNA; CDS; ncRNA
##Genome-Annotation-Data-END##
|
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
| ATGGGTGCGC ACCACCCGGC CCTCGGCCTG CTGCTGCTGC TGCTGTGCCC TGCGCAGGTG
TTTTCGCAAT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CGACTGGAGA TAAGAGGTAC
AACTGTAAAT ATTCTGGCCC ACCAAAACCC CTCCCAAAGG ACGGCTATGA CTTAGTGCAG
GAACTCTGTC CAGGACTCTT CTTTGACAAT GTCAGTCTCT GCTGTGACAT TCAACAGCTT
CAGACGCTGA AGAGTAACCT GCAGCTGCCC CTGCAGTTCC TGTCCAGGTG TCCGTCATGT
TTTTATAACC TAATGACCCT GTTTTGTGAG CTAACATGTA GCCCACACCA GAGTCAGTTT
CTGAATGTGA CAGCAACTGA GGATTATTTT GATCCTAAGA CACAGGAGAA TAAAACAAAT
GTAAAGGAAT TAGAGTACTT TGTCGGACAG AGCTTCGCGA ATGCGATGTA CAATGCCTGC
CGTGATGTGG AGGCGCCTTC CAGTAACGAG AAGGCCTTAG GACTCTTGTG TGGGAGGGAT
GCCCGTGCCT GCAATGCCAC CAACTGGATT GAGTACATGT TCAATAAAGA CAACGGACAA
GCGCCATTTA CCATCATTCC TGTGTTTTCA GATCTTTCAA TCCTTGGGAT GGAGCCCATG
AGAAATGCCA CCAAAGGCTG CAATGAGTCT GTAGATGAGG TCACGGGGCC ATGTAGCTGC
CAGGACTGCT CCATCGTCTG CGGCCCCAAG CCCCAGCCCC CACCCCCTCC TATGCCCTGG
AGGATCTGGG GCTTGGATGC CATGTATGTC ATCATGTGGG TCACCTACGT GGCATTTCTG
TTTGTGTTTT TTGGAGCACT GTTGGCAGTG TGGTGCCACA GGCGGTACTT TGTGTCTGAG
TACACTCCCA TTGACAGTAA CATCGCCTTT TCTGTGAATA GCAGTGACAA AGGGGAAGCC
TCATGCTGTG ACCCACTTGG TGCAGCATTT GATGACTGTC TGAGGCGCAT GTTCACAAAG
TGGGGGGCTT TCTGTGTCCG AAATCCCACC TGCATCATTT TCTTCTCATT GGCCTTCATC
ACTGTGTGCT CTTCTGGCCT GGTATTTGTC CAGGTCACCA CCAATCCTGT AGAGCTCTGG
TCAGCCCCTC ACAGTCAGGC CCGCTTGGAA AAGGAGTACT TTGACAAGCA CTTTGGGCCT
TTCTTTCGCA CGGAGCAGCT TATCATCCAG GCCCCCAACA CCAGTGTTCA TATCTACGAA
CCGTACCCCG CAGGAGCCGA TGTGCCCTTC GGGCCTCCAT TGAACAAAGA GATTCTGCAC
CAGGTTCTGG ACTTACAGAT CGCCATTGAA AGCATCACCG CATCTTACAA CAATGAAACC
GTGACACTGC AGGACATCTG TGTGGCCCCC CTCTCTCCAT ACAACAAGAA CTGCACCATT
ATGAGTGTGT TAAATTACTT CCAGAACAGC CATGCGGTGC TGGACAGCCA AGTAGGCGAC
GACTTCTATA TCTACGCTGA TTACCACACA CACTTTCTGT ACTGTGTACG GGCTCCCGCC
TCCTTGAATG ATACGAGTTT GCTCCACGGT CCTTGCCTGG GTACATTTGG AGGACCGGTG
TTCCCGTGGC TTGTGTTGGG TGGCTATGAT GATCAGAACT ACAACAATGC CACCGCGCTT
GTGATCACCT TCCCCGTGAA TAATTACTAC AATGACACAG AGAGGCTCCA GAGGGCCTGG
GCCTGGGAGA AAGAGTTTAT TAGTTTTGTG AAAAACTACA AGAATCCAAA TCTGACCATT
TCTTTCACTG CTGAGCGAAG CATCGAAGAT GAGCTCAATC GGGAAAGTAA CAGTGACGTG
TTCACCGTCA TCATCAGCTA CGTCGTGATG TTTCTGTACA TTTCCCTCGC CCTGGGTCAC
ATCCAGAGCT GCAGCAGGCT CCTGGTGGAT TCTAAGATCT CGCTGGGCAT TGCGGGGATC
CTGATCGTGC TAAGCTCGGT GGCCTGCTCT CTGGGCATCT TCAGCTACAT GGGGATGCCG
CTGACCCTCA TCGTCATTGA GGTCATCCCA TTCCTGGTGC TGGCTGTCGG GGTGGACAAC
ATCTTCATTC TAGTGCAGAC CTACCAGAGA GATGAGCGTC TTCAGGAGGA AACGCTGGAT
CAGCAGCTGG GCAGGATCCT TGGAGAAGTG GCCCCGACCA TGTTCCTTTC ATCCTTTTCT
GAGACCTCAG CATTTTTCTT TGGGGCGCTG TCCTCGATGC CAGCTGTGCA CACCTTCTCT
CTGTTTGCGG GAATGGCCGT CCTCATTGAC TTCCTCCTTC AGATTACCTG CTTTGTGAGC
CTGTTGGGGT TAGATATTAA GAGGCAAGAG AAAAACCATC TGGACATCCT GTGCTGTGTC
AGAGGCGCTG ACGACGGACA AGGTAGCCAC GCCTCCGAAA GCTACCTGTT TCGCTTCTTC
AAAAACTACT TTGCCCCCCT TCTGCTGAAG GACTGGCTGA GGCCAATTGT GGTAGCGGTG
TTTGTGGGCG TTCTGTCATT CAGTGTTGCG GTGGTGAACA AAGTAGACAT CGGGTTGGAT
CAGTCTCTCT CAATGCCAAA CGATTCGTAT GTGATTGACT ATTTCAAATC ACTCGCTCAG
TACCTGCACT CGGGCCCACC CGTGTACTTT GTCCTGGAGG AAGGCTATAA CTACAGTTCA
CGCAAAGGGC AGAACATGGT GTGCGGCGGC ATGGGCTGTG ACAATGACTC CCTGGTGCAG
CAGATATTTA ACGCAGCTGA GCTGGACACC TACACCCGAG TAGGCTTCGC CCCCTCGTCC
TGGATCGATG ACTACTTTGA CTGGGTCTCG CCACAGTCGT CCTGCTGCAG ACTCTACAAC
GTCACTCACC AGTTCTGCAA TGCTTCTGTG ATGGACCCAA CCTGTGTCCG CTGCAGACCT
CTGACTCCAG AGGGTAAACA GAGGCCTCAG GGGAAAGAAT TCATGAAATT CCTGCCCATG
TTCCTTTCTG ATAACCCCAA CCCCAAGTGC GGCAAAGGGG GACATGCTGC TTACGGTTCA
GCTGTTAACA TTGTGGGAGA TGACACTTAC ATTGGGGCCA CTTACTTCAT GACCTACCAC
ACCATACTTA AGACCTCCGC TGACTATACT GATGCCATGA AGAAAGCTCG GCTAATAGCC
AGTAACATCA CGGAAACCAT GCGTTCTAAG GGGAGTGACT ACCGCGTATT CCCTTACAGT
GTGTTCTACG TCTTCTATGA ACAGTACCTG ACCATTATTG ATGACACCAT CTTTAACCTC
AGTGTGTCTC TGGGCTCCAT ATTTCTGGTG ACCTTGGTGG TTCTGGGCTG TGAGCTGTGG
TCTGCGGTCA TCATGTGTAT CACCATAGCC ATGATCCTGG TCAACATGTT CGGTGTCATG
TGGCTGTGGG GCATCAGTCT GAATGCGGTC TCCTTGGTCA ACTTGGTGAT GAGCTGCGGC
ATTTCTGTGG AGTTCTGCAG CCATATAACG AGAGCATTCA CCATGAGTAC CAAAGGAAGC
CGAGTGAGCC GGGCGGAAGA GGCACTGGCC CACATGGGTA GTTCTGTATT CAGTGGAATC
ACACTTACGA AATTTGGAGG GATCGTGGTG TTAGCCTTTG CCAAATCTCA AATTTTTGAG
ATATTTTACT TCAGGATGTA CTTAGCCATG GTCTTACTCG GAGCCACTCA TGGACTAATA
TTTCTTCCCG TCTTACTCAG TTACATAGGG CCGTCGGTGA ATAAAGCTAA AAGACACACC
ACTTACGAGC GCTACAGAGG GACAGAGAGA GAACGGCTCC TCAATTTTTA G |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq |
XP_017173338.1 |
| CDS | 196..4026 |
| Translation | |
Target ORF information:
| RefSeq Version | XM_017317849.1 |
| Organism | Mus musculus(house mouse) |
| Definition | Mus musculus Niemann-Pick type C1 (Npc1), transcript variant X1, mRNA. |
Target ORF information:
| Epitope | DYKDDDDK |
| Bacterial selection | AMPR |
| Mammalian selection | NeoR |
| Vector | pcDNA3.1+/C-(K)DYK |
|
 XM_017317849.1 |
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
| ATGGGTGCGC ACCACCCGGC CCTCGGCCTG CTGCTGCTGC TGCTGTGCCC TGCGCAGGTG
TTTTCGCAAT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CGACTGGAGA TAAGAGGTAC
AACTGTAAAT ATTCTGGCCC ACCAAAACCC CTCCCAAAGG ACGGCTATGA CTTAGTGCAG
GAACTCTGTC CAGGACTCTT CTTTGACAAT GTCAGTCTCT GCTGTGACAT TCAACAGCTT
CAGACGCTGA AGAGTAACCT GCAGCTGCCC CTGCAGTTCC TGTCCAGGTG TCCGTCATGT
TTTTATAACC TAATGACCCT GTTTTGTGAG CTAACATGTA GCCCACACCA GAGTCAGTTT
CTGAATGTGA CAGCAACTGA GGATTATTTT GATCCTAAGA CACAGGAGAA TAAAACAAAT
GTAAAGGAAT TAGAGTACTT TGTCGGACAG AGCTTCGCGA ATGCGATGTA CAATGCCTGC
CGTGATGTGG AGGCGCCTTC CAGTAACGAG AAGGCCTTAG GACTCTTGTG TGGGAGGGAT
GCCCGTGCCT GCAATGCCAC CAACTGGATT GAGTACATGT TCAATAAAGA CAACGGACAA
GCGCCATTTA CCATCATTCC TGTGTTTTCA GATCTTTCAA TCCTTGGGAT GGAGCCCATG
AGAAATGCCA CCAAAGGCTG CAATGAGTCT GTAGATGAGG TCACGGGGCC ATGTAGCTGC
CAGGACTGCT CCATCGTCTG CGGCCCCAAG CCCCAGCCCC CACCCCCTCC TATGCCCTGG
AGGATCTGGG GCTTGGATGC CATGTATGTC ATCATGTGGG TCACCTACGT GGCATTTCTG
TTTGTGTTTT TTGGAGCACT GTTGGCAGTG TGGTGCCACA GGCGGTACTT TGTGTCTGAG
TACACTCCCA TTGACAGTAA CATCGCCTTT TCTGTGAATA GCAGTGACAA AGGGGAAGCC
TCATGCTGTG ACCCACTTGG TGCAGCATTT GATGACTGTC TGAGGCGCAT GTTCACAAAG
TGGGGGGCTT TCTGTGTCCG AAATCCCACC TGCATCATTT TCTTCTCATT GGCCTTCATC
ACTGTGTGCT CTTCTGGCCT GGTATTTGTC CAGGTCACCA CCAATCCTGT AGAGCTCTGG
TCAGCCCCTC ACAGTCAGGC CCGCTTGGAA AAGGAGTACT TTGACAAGCA CTTTGGGCCT
TTCTTTCGCA CGGAGCAGCT TATCATCCAG GCCCCCAACA CCAGTGTTCA TATCTACGAA
CCGTACCCCG CAGGAGCCGA TGTGCCCTTC GGGCCTCCAT TGAACAAAGA GATTCTGCAC
CAGGTTCTGG ACTTACAGAT CGCCATTGAA AGCATCACCG CATCTTACAA CAATGAAACC
GTGACACTGC AGGACATCTG TGTGGCCCCC CTCTCTCCAT ACAACAAGAA CTGCACCATT
ATGAGTGTGT TAAATTACTT CCAGAACAGC CATGCGGTGC TGGACAGCCA AGTAGGCGAC
GACTTCTATA TCTACGCTGA TTACCACACA CACTTTCTGT ACTGTGTACG GGCTCCCGCC
TCCTTGAATG ATACGAGTTT GCTCCACGGT CCTTGCCTGG GTACATTTGG AGGACCGGTG
TTCCCGTGGC TTGTGTTGGG TGGCTATGAT GATCAGAACT ACAACAATGC CACCGCGCTT
GTGATCACCT TCCCCGTGAA TAATTACTAC AATGACACAG AGAGGCTCCA GAGGGCCTGG
GCCTGGGAGA AAGAGTTTAT TAGTTTTGTG AAAAACTACA AGAATCCAAA TCTGACCATT
TCTTTCACTG CTGAGCGAAG CATCGAAGAT GAGCTCAATC GGGAAAGTAA CAGTGACGTG
TTCACCGTCA TCATCAGCTA CGTCGTGATG TTTCTGTACA TTTCCCTCGC CCTGGGTCAC
ATCCAGAGCT GCAGCAGGCT CCTGGTGGAT TCTAAGATCT CGCTGGGCAT TGCGGGGATC
CTGATCGTGC TAAGCTCGGT GGCCTGCTCT CTGGGCATCT TCAGCTACAT GGGGATGCCG
CTGACCCTCA TCGTCATTGA GGTCATCCCA TTCCTGGTGC TGGCTGTCGG GGTGGACAAC
ATCTTCATTC TAGTGCAGAC CTACCAGAGA GATGAGCGTC TTCAGGAGGA AACGCTGGAT
CAGCAGCTGG GCAGGATCCT TGGAGAAGTG GCCCCGACCA TGTTCCTTTC ATCCTTTTCT
GAGACCTCAG CATTTTTCTT TGGGGCGCTG TCCTCGATGC CAGCTGTGCA CACCTTCTCT
CTGTTTGCGG GAATGGCCGT CCTCATTGAC TTCCTCCTTC AGATTACCTG CTTTGTGAGC
CTGTTGGGGT TAGATATTAA GAGGCAAGAG AAAAACCATC TGGACATCCT GTGCTGTGTC
AGAGGCGCTG ACGACGGACA AGGTAGCCAC GCCTCCGAAA GCTACCTGTT TCGCTTCTTC
AAAAACTACT TTGCCCCCCT TCTGCTGAAG GACTGGCTGA GGCCAATTGT GGTAGCGGTG
TTTGTGGGCG TTCTGTCATT CAGTGTTGCG GTGGTGAACA AAGTAGACAT CGGGTTGGAT
CAGTCTCTCT CAATGCCAAA CGATTCGTAT GTGATTGACT ATTTCAAATC ACTCGCTCAG
TACCTGCACT CGGGCCCACC CGTGTACTTT GTCCTGGAGG AAGGCTATAA CTACAGTTCA
CGCAAAGGGC AGAACATGGT GTGCGGCGGC ATGGGCTGTG ACAATGACTC CCTGGTGCAG
CAGATATTTA ACGCAGCTGA GCTGGACACC TACACCCGAG TAGGCTTCGC CCCCTCGTCC
TGGATCGATG ACTACTTTGA CTGGGTCTCG CCACAGTCGT CCTGCTGCAG ACTCTACAAC
GTCACTCACC AGTTCTGCAA TGCTTCTGTG ATGGACCCAA CCTGTGTCCG CTGCAGACCT
CTGACTCCAG AGGGTAAACA GAGGCCTCAG GGGAAAGAAT TCATGAAATT CCTGCCCATG
TTCCTTTCTG ATAACCCCAA CCCCAAGTGC GGCAAAGGGG GACATGCTGC TTACGGTTCA
GCTGTTAACA TTGTGGGAGA TGACACTTAC ATTGGGGCCA CTTACTTCAT GACCTACCAC
ACCATACTTA AGACCTCCGC TGACTATACT GATGCCATGA AGAAAGCTCG GCTAATAGCC
AGTAACATCA CGGAAACCAT GCGTTCTAAG GGGAGTGACT ACCGCGTATT CCCTTACAGT
GTGTTCTACG TCTTCTATGA ACAGTACCTG ACCATTATTG ATGACACCAT CTTTAACCTC
AGTGTGTCTC TGGGCTCCAT ATTTCTGGTG ACCTTGGTGG TTCTGGGCTG TGAGCTGTGG
TCTGCGGTCA TCATGTGTAT CACCATAGCC ATGATCCTGG TCAACATGTT CGGTGTCATG
TGGCTGTGGG GCATCAGTCT GAATGCGGTC TCCTTGGTCA ACTTGGTGAT GAGCTGCGGC
ATTTCTGTGG AGTTCTGCAG CCATATAACG AGAGCATTCA CCATGAGTAC CAAAGGAAGC
CGAGTGAGCC GGGCGGAAGA GGCACTGGCC CACATGGGTA GTTCTGTATT CAGTGGAATC
ACACTTACGA AATTTGGAGG GATCGTGGTG TTAGCCTTTG CCAAATCTCA AATTTTTGAG
ATATTTTACT TCAGGATGTA CTTAGCCATG GTCTTACTCG GAGCCACTCA TGGACTAATA
TTTCTTCCCG TCTTACTCAG TTACATAGGG CCGTCGGTGA ATAAAGCTAA AAGACACACC
ACTTACGAGC GCTACAGAGG GACAGAGAGA GAACGGCTCC TCAATTTTTA G |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID |
OMu21708 |
| Clone ID Related Accession (Same CDS sequence) |
NM_008720.2
|
| Accession Version |
NM_008720.2 Latest version! |
Documents for ORF clone product in default vector |
| Sequence Information |
ORF Nucleotide Sequence (Length: 3834bp)
Protein sequence
SNP |
| Vector |
pcDNA3.1+/C-(K)DYK or customized vector |
User Manual |
| Clone information |
Clone Map |
MSDS |
| Tag on pcDNA3.1+/C-(K)DYK |
C terminal DYKDDDDK tags |
| ORF Insert Method |
CloneEZ™ Seamless cloning technology |
| Insert Structure |
linear |
| Update Date |
2019-06-25 |
| Organism |
Mus musculus(house mouse) |
| Product |
NPC intracellular cholesterol transporter 1 precursor |
| Comment |
Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC052437.1, CF585180.1 and AK003935.1. On Mar 7, 2006 this sequence version replaced NM_008720.1.
Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.
##Evidence-Data-START##
Transcript exon combination :: BC052437.1, AF003348.1 [ECO:0000332]
RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350]
##Evidence-Data-END##
|
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
| ATGGGTGCGC ACCACCCGGC CCTCGGCCTG CTGCTGCTGC TGCTGTGCCC TGCGCAGGTG
TTTTCGCAAT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CGACTGGAGA TAAGAGGTAC
AACTGTAAAT ATTCTGGCCC ACCAAAACCC CTCCCAAAGG ACGGCTATGA CTTAGTGCAG
GAACTCTGTC CAGGACTCTT CTTTGACAAT GTCAGTCTCT GCTGTGACAT TCAACAGCTT
CAGACGCTGA AGAGTAACCT GCAGCTGCCC CTGCAGTTCC TGTCCAGGTG TCCGTCATGT
TTTTATAACC TAATGACCCT GTTTTGTGAG CTAACATGTA GCCCACACCA GAGTCAGTTT
CTGAATGTGA CAGCAACTGA GGATTATTTT GATCCTAAGA CACAGGAGAA TAAAACAAAT
GTAAAGGAAT TAGAGTACTT TGTCGGACAG AGCTTCGCGA ATGCGATGTA CAATGCCTGC
CGTGATGTGG AGGCGCCTTC CAGTAACGAG AAGGCCTTAG GACTCTTGTG TGGGAGGGAT
GCCCGTGCCT GCAATGCCAC CAACTGGATT GAGTACATGT TCAATAAAGA CAACGGACAA
GCGCCATTTA CCATCATTCC TGTGTTTTCA GATCTTTCAA TCCTTGGGAT GGAGCCCATG
AGAAATGCCA CCAAAGGCTG CAATGAGTCT GTAGATGAGG TCACGGGGCC ATGTAGCTGC
CAGGACTGCT CCATCGTCTG CGGCCCCAAG CCCCAGCCCC CACCCCCTCC TATGCCCTGG
AGGATCTGGG GCTTGGATGC CATGTATGTC ATCATGTGGG TCACCTACGT GGCATTTCTG
TTTGTGTTTT TTGGAGCACT GTTGGCAGTG TGGTGCCACA GAAGGCGGTA CTTTGTGTCT
GAGTACACTC CCATTGACAG TAACATCGCC TTTTCTGTGA ATAGCAGTGA CAAAGGGGAA
GCCTCATGCT GTGACCCACT TGGTGCAGCA TTTGATGACT GTCTGAGGCG CATGTTCACA
AAGTGGGGGG CTTTCTGTGT CCGAAATCCC ACCTGCATCA TTTTCTTCTC ATTGGCCTTC
ATCACTGTGT GCTCTTCTGG CCTGGTATTT GTCCAGGTCA CCACCAATCC TGTAGAGCTC
TGGTCAGCCC CTCACAGTCA GGCCCGCTTG GAAAAGGAGT ACTTTGACAA GCACTTTGGG
CCTTTCTTTC GCACGGAGCA GCTTATCATC CAGGCCCCCA ACACCAGTGT TCATATCTAC
GAACCGTACC CCGCAGGAGC CGATGTGCCC TTCGGGCCTC CATTGAACAA AGAGATTCTG
CACCAGGTTC TGGACTTACA GATCGCCATT GAAAGCATCA CCGCATCTTA CAACAATGAA
ACCGTGACAC TGCAGGACAT CTGTGTGGCC CCCCTCTCTC CATACAACAA GAACTGCACC
ATTATGAGTG TGTTAAATTA CTTCCAGAAC AGCCATGCGG TGCTGGACAG CCAAGTAGGC
GACGACTTCT ATATCTACGC TGATTACCAC ACACACTTTC TGTACTGTGT ACGGGCTCCC
GCCTCCTTGA ATGATACGAG TTTGCTCCAC GGTCCTTGCC TGGGTACATT TGGAGGACCG
GTGTTCCCGT GGCTTGTGTT GGGTGGCTAT GATGATCAGA ACTACAACAA TGCCACCGCG
CTTGTGATCA CCTTCCCCGT GAATAATTAC TACAATGACA CAGAGAGGCT CCAGAGGGCC
TGGGCCTGGG AGAAAGAGTT TATTAGTTTT GTGAAAAACT ACAAGAATCC AAATCTGACC
ATTTCTTTCA CTGCTGAGCG AAGCATCGAA GATGAGCTCA ATCGGGAAAG TAACAGTGAC
GTGTTCACCG TCATCATCAG CTACGTCGTG ATGTTTCTGT ACATTTCCCT CGCCCTGGGT
CACATCCAGA GCTGCAGCAG GCTCCTGGTG GATTCTAAGA TCTCGCTGGG CATTGCGGGG
ATCCTGATCG TGCTAAGCTC GGTGGCCTGC TCTCTGGGCA TCTTCAGCTA CATGGGGATG
CCGCTGACCC TCATCGTCAT TGAGGTCATC CCATTCCTGG TGCTGGCTGT CGGGGTGGAC
AACATCTTCA TTCTAGTGCA GACCTACCAG AGAGATGAGC GTCTTCAGGA GGAAACGCTG
GATCAGCAGC TGGGCAGGAT CCTTGGAGAA GTGGCCCCGA CCATGTTCCT TTCATCCTTT
TCTGAGACCT CAGCATTTTT CTTTGGGGCG CTGTCCTCGA TGCCAGCTGT GCACACCTTC
TCTCTGTTTG CGGGAATGGC CGTCCTCATT GACTTCCTCC TTCAGATTAC CTGCTTTGTG
AGCCTGTTGG GGTTAGATAT TAAGAGGCAA GAGAAAAACC ATCTGGACAT CCTGTGCTGT
GTCAGAGGCG CTGACGACGG ACAAGGTAGC CACGCCTCCG AAAGCTACCT GTTTCGCTTC
TTCAAAAACT ACTTTGCCCC CCTTCTGCTG AAGGACTGGC TGAGGCCAAT TGTGGTAGCG
GTGTTTGTGG GCGTTCTGTC ATTCAGTGTT GCGGTGGTGA ACAAAGTAGA CATCGGGTTG
GATCAGTCTC TCTCAATGCC AAACGATTCG TATGTGATTG ACTATTTCAA ATCACTCGCT
CAGTACCTGC ACTCGGGCCC ACCCGTGTAC TTTGTCCTGG AGGAAGGCTA TAACTACAGT
TCACGCAAAG GGCAGAACAT GGTGTGCGGC GGCATGGGCT GTGACAATGA CTCCCTGGTG
CAGCAGATAT TTAACGCAGC TGAGCTGGAC ACCTACACCC GAGTAGGCTT CGCCCCCTCG
TCCTGGATCG ATGACTACTT TGACTGGGTC TCGCCACAGT CGTCCTGCTG CAGACTCTAC
AACGTCACTC ACCAGTTCTG CAATGCTTCT GTGATGGACC CAACCTGTGT CCGCTGCAGA
CCTCTGACTC CAGAGGGTAA ACAGAGGCCT CAGGGGAAAG AATTCATGAA ATTCCTGCCC
ATGTTCCTTT CTGATAACCC CAACCCCAAG TGCGGCAAAG GGGGACATGC TGCTTACGGT
TCAGCTGTTA ACATTGTGGG AGATGACACT TACATTGGGG CCACTTACTT CATGACCTAC
CACACCATAC TTAAGACCTC CGCTGACTAT ACTGATGCCA TGAAGAAAGC TCGGCTAATA
GCCAGTAACA TCACGGAAAC CATGCGTTCT AAGGGGAGTG ACTACCGCGT ATTCCCTTAC
AGTGTGTTCT ACGTCTTCTA TGAACAGTAC CTGACCATTA TTGATGACAC CATCTTTAAC
CTCAGTGTGT CTCTGGGCTC CATATTTCTG GTGACCTTGG TGGTTCTGGG CTGTGAGCTG
TGGTCTGCGG TCATCATGTG TATCACCATA GCCATGATCC TGGTCAACAT GTTCGGTGTC
ATGTGGCTGT GGGGCATCAG TCTGAATGCG GTCTCCTTGG TCAACTTGGT GATGAGCTGC
GGCATTTCTG TGGAGTTCTG CAGCCATATA ACGAGAGCAT TCACCATGAG TACCAAAGGA
AGCCGAGTGA GCCGGGCGGA AGAGGCACTG GCCCACATGG GTAGTTCTGT ATTCAGTGGA
ATCACACTTA CGAAATTTGG AGGGATCGTG GTGTTAGCCT TTGCCAAATC TCAAATTTTT
GAGATATTTT ACTTCAGGAT GTACTTAGCC ATGGTCTTAC TCGGAGCCAC TCATGGACTA
ATATTTCTTC CCGTCTTACT CAGTTACATA GGGCCGTCGG TGAATAAAGC TAAAAGACAC
ACCACTTACG AGCGCTACAG AGGGACAGAG AGAGAACGGC TCCTCAATTT TTAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq |
NP_032746.2 |
| CDS | 150..3983 |
| Translation | |
Target ORF information:
| RefSeq Version | NM_008720.2 |
| Organism | Mus musculus(house mouse) |
| Definition | Mus musculus NPC intracellular cholesterol transporter 1 (Npc1), mRNA. |
Target ORF information:
| Epitope | DYKDDDDK |
| Bacterial selection | AMPR |
| Mammalian selection | NeoR |
| Vector | pcDNA3.1+/C-(K)DYK |
|
NM_008720.2 |
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
| ATGGGTGCGC ACCACCCGGC CCTCGGCCTG CTGCTGCTGC TGCTGTGCCC TGCGCAGGTG
TTTTCGCAAT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CGACTGGAGA TAAGAGGTAC
AACTGTAAAT ATTCTGGCCC ACCAAAACCC CTCCCAAAGG ACGGCTATGA CTTAGTGCAG
GAACTCTGTC CAGGACTCTT CTTTGACAAT GTCAGTCTCT GCTGTGACAT TCAACAGCTT
CAGACGCTGA AGAGTAACCT GCAGCTGCCC CTGCAGTTCC TGTCCAGGTG TCCGTCATGT
TTTTATAACC TAATGACCCT GTTTTGTGAG CTAACATGTA GCCCACACCA GAGTCAGTTT
CTGAATGTGA CAGCAACTGA GGATTATTTT GATCCTAAGA CACAGGAGAA TAAAACAAAT
GTAAAGGAAT TAGAGTACTT TGTCGGACAG AGCTTCGCGA ATGCGATGTA CAATGCCTGC
CGTGATGTGG AGGCGCCTTC CAGTAACGAG AAGGCCTTAG GACTCTTGTG TGGGAGGGAT
GCCCGTGCCT GCAATGCCAC CAACTGGATT GAGTACATGT TCAATAAAGA CAACGGACAA
GCGCCATTTA CCATCATTCC TGTGTTTTCA GATCTTTCAA TCCTTGGGAT GGAGCCCATG
AGAAATGCCA CCAAAGGCTG CAATGAGTCT GTAGATGAGG TCACGGGGCC ATGTAGCTGC
CAGGACTGCT CCATCGTCTG CGGCCCCAAG CCCCAGCCCC CACCCCCTCC TATGCCCTGG
AGGATCTGGG GCTTGGATGC CATGTATGTC ATCATGTGGG TCACCTACGT GGCATTTCTG
TTTGTGTTTT TTGGAGCACT GTTGGCAGTG TGGTGCCACA GAAGGCGGTA CTTTGTGTCT
GAGTACACTC CCATTGACAG TAACATCGCC TTTTCTGTGA ATAGCAGTGA CAAAGGGGAA
GCCTCATGCT GTGACCCACT TGGTGCAGCA TTTGATGACT GTCTGAGGCG CATGTTCACA
AAGTGGGGGG CTTTCTGTGT CCGAAATCCC ACCTGCATCA TTTTCTTCTC ATTGGCCTTC
ATCACTGTGT GCTCTTCTGG CCTGGTATTT GTCCAGGTCA CCACCAATCC TGTAGAGCTC
TGGTCAGCCC CTCACAGTCA GGCCCGCTTG GAAAAGGAGT ACTTTGACAA GCACTTTGGG
CCTTTCTTTC GCACGGAGCA GCTTATCATC CAGGCCCCCA ACACCAGTGT TCATATCTAC
GAACCGTACC CCGCAGGAGC CGATGTGCCC TTCGGGCCTC CATTGAACAA AGAGATTCTG
CACCAGGTTC TGGACTTACA GATCGCCATT GAAAGCATCA CCGCATCTTA CAACAATGAA
ACCGTGACAC TGCAGGACAT CTGTGTGGCC CCCCTCTCTC CATACAACAA GAACTGCACC
ATTATGAGTG TGTTAAATTA CTTCCAGAAC AGCCATGCGG TGCTGGACAG CCAAGTAGGC
GACGACTTCT ATATCTACGC TGATTACCAC ACACACTTTC TGTACTGTGT ACGGGCTCCC
GCCTCCTTGA ATGATACGAG TTTGCTCCAC GGTCCTTGCC TGGGTACATT TGGAGGACCG
GTGTTCCCGT GGCTTGTGTT GGGTGGCTAT GATGATCAGA ACTACAACAA TGCCACCGCG
CTTGTGATCA CCTTCCCCGT GAATAATTAC TACAATGACA CAGAGAGGCT CCAGAGGGCC
TGGGCCTGGG AGAAAGAGTT TATTAGTTTT GTGAAAAACT ACAAGAATCC AAATCTGACC
ATTTCTTTCA CTGCTGAGCG AAGCATCGAA GATGAGCTCA ATCGGGAAAG TAACAGTGAC
GTGTTCACCG TCATCATCAG CTACGTCGTG ATGTTTCTGT ACATTTCCCT CGCCCTGGGT
CACATCCAGA GCTGCAGCAG GCTCCTGGTG GATTCTAAGA TCTCGCTGGG CATTGCGGGG
ATCCTGATCG TGCTAAGCTC GGTGGCCTGC TCTCTGGGCA TCTTCAGCTA CATGGGGATG
CCGCTGACCC TCATCGTCAT TGAGGTCATC CCATTCCTGG TGCTGGCTGT CGGGGTGGAC
AACATCTTCA TTCTAGTGCA GACCTACCAG AGAGATGAGC GTCTTCAGGA GGAAACGCTG
GATCAGCAGC TGGGCAGGAT CCTTGGAGAA GTGGCCCCGA CCATGTTCCT TTCATCCTTT
TCTGAGACCT CAGCATTTTT CTTTGGGGCG CTGTCCTCGA TGCCAGCTGT GCACACCTTC
TCTCTGTTTG CGGGAATGGC CGTCCTCATT GACTTCCTCC TTCAGATTAC CTGCTTTGTG
AGCCTGTTGG GGTTAGATAT TAAGAGGCAA GAGAAAAACC ATCTGGACAT CCTGTGCTGT
GTCAGAGGCG CTGACGACGG ACAAGGTAGC CACGCCTCCG AAAGCTACCT GTTTCGCTTC
TTCAAAAACT ACTTTGCCCC CCTTCTGCTG AAGGACTGGC TGAGGCCAAT TGTGGTAGCG
GTGTTTGTGG GCGTTCTGTC ATTCAGTGTT GCGGTGGTGA ACAAAGTAGA CATCGGGTTG
GATCAGTCTC TCTCAATGCC AAACGATTCG TATGTGATTG ACTATTTCAA ATCACTCGCT
CAGTACCTGC ACTCGGGCCC ACCCGTGTAC TTTGTCCTGG AGGAAGGCTA TAACTACAGT
TCACGCAAAG GGCAGAACAT GGTGTGCGGC GGCATGGGCT GTGACAATGA CTCCCTGGTG
CAGCAGATAT TTAACGCAGC TGAGCTGGAC ACCTACACCC GAGTAGGCTT CGCCCCCTCG
TCCTGGATCG ATGACTACTT TGACTGGGTC TCGCCACAGT CGTCCTGCTG CAGACTCTAC
AACGTCACTC ACCAGTTCTG CAATGCTTCT GTGATGGACC CAACCTGTGT CCGCTGCAGA
CCTCTGACTC CAGAGGGTAA ACAGAGGCCT CAGGGGAAAG AATTCATGAA ATTCCTGCCC
ATGTTCCTTT CTGATAACCC CAACCCCAAG TGCGGCAAAG GGGGACATGC TGCTTACGGT
TCAGCTGTTA ACATTGTGGG AGATGACACT TACATTGGGG CCACTTACTT CATGACCTAC
CACACCATAC TTAAGACCTC CGCTGACTAT ACTGATGCCA TGAAGAAAGC TCGGCTAATA
GCCAGTAACA TCACGGAAAC CATGCGTTCT AAGGGGAGTG ACTACCGCGT ATTCCCTTAC
AGTGTGTTCT ACGTCTTCTA TGAACAGTAC CTGACCATTA TTGATGACAC CATCTTTAAC
CTCAGTGTGT CTCTGGGCTC CATATTTCTG GTGACCTTGG TGGTTCTGGG CTGTGAGCTG
TGGTCTGCGG TCATCATGTG TATCACCATA GCCATGATCC TGGTCAACAT GTTCGGTGTC
ATGTGGCTGT GGGGCATCAG TCTGAATGCG GTCTCCTTGG TCAACTTGGT GATGAGCTGC
GGCATTTCTG TGGAGTTCTG CAGCCATATA ACGAGAGCAT TCACCATGAG TACCAAAGGA
AGCCGAGTGA GCCGGGCGGA AGAGGCACTG GCCCACATGG GTAGTTCTGT ATTCAGTGGA
ATCACACTTA CGAAATTTGG AGGGATCGTG GTGTTAGCCT TTGCCAAATC TCAAATTTTT
GAGATATTTT ACTTCAGGAT GTACTTAGCC ATGGTCTTAC TCGGAGCCAC TCATGGACTA
ATATTTCTTC CCGTCTTACT CAGTTACATA GGGCCGTCGG TGAATAAAGC TAAAAGACAC
ACCACTTACG AGCGCTACAG AGGGACAGAG AGAGAACGGC TCCTCAATTT TTAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
