Npc1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Npc1
Entrez Gene ID	18145
Full Name	NPC intracellular cholesterol transporter 1
Synonyms	A430089E03Rik,C85354,D18Ertd139e,D18Ertd723e,lcsd,nmf164,spm
General protein information	Preferred Names NPC intracellular cholesterol transporter 1 Names Niemann-Pick C1 protein Niemann-Pick type C1 sphingomyelinosis
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18 A1\|18 6.15 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_008720.2	NP_032746.2	NPC intracellular cholesterol transporter 1 precursor
XM_017317849.1	XP_017173338.1	Niemann-Pick C1 protein isoform X1
XM_017317849.2	XP_017173338.1	NPC intracellular cholesterol transporter 1 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NPC1	NP_001003107.1
Bos taurus (cattle)	NPC1	NP_777183.1
Kluyveromyces lactis	KLLA0F27137g	XP_456287.1
Magnaporthe oryzae (rice blast fungus)	MGG_00819	XP_003718164.1
Mus musculus (house mouse)	Npc1	NP_032746.2
Drosophila melanogaster (fruit fly)	Npc1a	NP_001188769.1
Homo sapiens (human)	NPC1	NP_000262.2
Pan troglodytes (chimpanzee)	NPC1	XP_001155285.1
	NPC1	XP_002800934.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008137	XP_317322.4
Rattus norvegicus (Norway rat)	Npc1	NP_705888.2
Danio rerio (zebrafish)	npc1	NP_001230804.1
Saccharomyces cerevisiae (baker's yeast)	NCR1	NP_015319.1
Eremothecium gossypii	AGOS_AGL008W	NP_986658.1
Neurospora crassa	NCU04090	XP_956086.1
Arabidopsis thaliana (thale cress)	AT1G42470	NP_174975.2
Gallus gallus (chicken)	NPC1	XP_419162.3
Arabidopsis thaliana (thale cress)	AT4G38350	NP_001190953.1
Xenopus tropicalis (tropical clawed frog)	npc1	XP_004915269.1

Gene Ontology

The following Npc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Npc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu79770	XM_017317849.2 Latest version!	Mus musculus NPC intracellular cholesterol transporter 1 (Npc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu21708	NM_008720.2 Latest version!	Mus musculus NPC intracellular cholesterol transporter 1 (Npc1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$748.30 $1069.00
View Old Accession Versions >>
OMu79770	XM_017317849.1	Mus musculus Niemann-Pick type C1 (Npc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Npc1 ( XM_017317849.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173338.1 Mus musculus Niemann-Pick type C1 (Npc1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu79770
Clone ID Related Accession (Same CDS sequence)	XM_017317849.1 , XM_017317849.2
Accession Version	XM_017317849.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3831bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	Niemann-Pick C1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGCGC ACCACCCGGC CCTCGGCCTG CTGCTGCTGC TGCTGTGCCC TGCGCAGGTG 
TTTTCGCAAT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CGACTGGAGA TAAGAGGTAC 
AACTGTAAAT ATTCTGGCCC ACCAAAACCC CTCCCAAAGG ACGGCTATGA CTTAGTGCAG 
GAACTCTGTC CAGGACTCTT CTTTGACAAT GTCAGTCTCT GCTGTGACAT TCAACAGCTT 
CAGACGCTGA AGAGTAACCT GCAGCTGCCC CTGCAGTTCC TGTCCAGGTG TCCGTCATGT 
TTTTATAACC TAATGACCCT GTTTTGTGAG CTAACATGTA GCCCACACCA GAGTCAGTTT 
CTGAATGTGA CAGCAACTGA GGATTATTTT GATCCTAAGA CACAGGAGAA TAAAACAAAT 
GTAAAGGAAT TAGAGTACTT TGTCGGACAG AGCTTCGCGA ATGCGATGTA CAATGCCTGC 
CGTGATGTGG AGGCGCCTTC CAGTAACGAG AAGGCCTTAG GACTCTTGTG TGGGAGGGAT 
GCCCGTGCCT GCAATGCCAC CAACTGGATT GAGTACATGT TCAATAAAGA CAACGGACAA 
GCGCCATTTA CCATCATTCC TGTGTTTTCA GATCTTTCAA TCCTTGGGAT GGAGCCCATG 
AGAAATGCCA CCAAAGGCTG CAATGAGTCT GTAGATGAGG TCACGGGGCC ATGTAGCTGC 
CAGGACTGCT CCATCGTCTG CGGCCCCAAG CCCCAGCCCC CACCCCCTCC TATGCCCTGG 
AGGATCTGGG GCTTGGATGC CATGTATGTC ATCATGTGGG TCACCTACGT GGCATTTCTG 
TTTGTGTTTT TTGGAGCACT GTTGGCAGTG TGGTGCCACA GGCGGTACTT TGTGTCTGAG 
TACACTCCCA TTGACAGTAA CATCGCCTTT TCTGTGAATA GCAGTGACAA AGGGGAAGCC 
TCATGCTGTG ACCCACTTGG TGCAGCATTT GATGACTGTC TGAGGCGCAT GTTCACAAAG 
TGGGGGGCTT TCTGTGTCCG AAATCCCACC TGCATCATTT TCTTCTCATT GGCCTTCATC 
ACTGTGTGCT CTTCTGGCCT GGTATTTGTC CAGGTCACCA CCAATCCTGT AGAGCTCTGG 
TCAGCCCCTC ACAGTCAGGC CCGCTTGGAA AAGGAGTACT TTGACAAGCA CTTTGGGCCT 
TTCTTTCGCA CGGAGCAGCT TATCATCCAG GCCCCCAACA CCAGTGTTCA TATCTACGAA 
CCGTACCCCG CAGGAGCCGA TGTGCCCTTC GGGCCTCCAT TGAACAAAGA GATTCTGCAC 
CAGGTTCTGG ACTTACAGAT CGCCATTGAA AGCATCACCG CATCTTACAA CAATGAAACC 
GTGACACTGC AGGACATCTG TGTGGCCCCC CTCTCTCCAT ACAACAAGAA CTGCACCATT 
ATGAGTGTGT TAAATTACTT CCAGAACAGC CATGCGGTGC TGGACAGCCA AGTAGGCGAC 
GACTTCTATA TCTACGCTGA TTACCACACA CACTTTCTGT ACTGTGTACG GGCTCCCGCC 
TCCTTGAATG ATACGAGTTT GCTCCACGGT CCTTGCCTGG GTACATTTGG AGGACCGGTG 
TTCCCGTGGC TTGTGTTGGG TGGCTATGAT GATCAGAACT ACAACAATGC CACCGCGCTT 
GTGATCACCT TCCCCGTGAA TAATTACTAC AATGACACAG AGAGGCTCCA GAGGGCCTGG 
GCCTGGGAGA AAGAGTTTAT TAGTTTTGTG AAAAACTACA AGAATCCAAA TCTGACCATT 
TCTTTCACTG CTGAGCGAAG CATCGAAGAT GAGCTCAATC GGGAAAGTAA CAGTGACGTG 
TTCACCGTCA TCATCAGCTA CGTCGTGATG TTTCTGTACA TTTCCCTCGC CCTGGGTCAC 
ATCCAGAGCT GCAGCAGGCT CCTGGTGGAT TCTAAGATCT CGCTGGGCAT TGCGGGGATC 
CTGATCGTGC TAAGCTCGGT GGCCTGCTCT CTGGGCATCT TCAGCTACAT GGGGATGCCG 
CTGACCCTCA TCGTCATTGA GGTCATCCCA TTCCTGGTGC TGGCTGTCGG GGTGGACAAC 
ATCTTCATTC TAGTGCAGAC CTACCAGAGA GATGAGCGTC TTCAGGAGGA AACGCTGGAT 
CAGCAGCTGG GCAGGATCCT TGGAGAAGTG GCCCCGACCA TGTTCCTTTC ATCCTTTTCT 
GAGACCTCAG CATTTTTCTT TGGGGCGCTG TCCTCGATGC CAGCTGTGCA CACCTTCTCT 
CTGTTTGCGG GAATGGCCGT CCTCATTGAC TTCCTCCTTC AGATTACCTG CTTTGTGAGC 
CTGTTGGGGT TAGATATTAA GAGGCAAGAG AAAAACCATC TGGACATCCT GTGCTGTGTC 
AGAGGCGCTG ACGACGGACA AGGTAGCCAC GCCTCCGAAA GCTACCTGTT TCGCTTCTTC 
AAAAACTACT TTGCCCCCCT TCTGCTGAAG GACTGGCTGA GGCCAATTGT GGTAGCGGTG 
TTTGTGGGCG TTCTGTCATT CAGTGTTGCG GTGGTGAACA AAGTAGACAT CGGGTTGGAT 
CAGTCTCTCT CAATGCCAAA CGATTCGTAT GTGATTGACT ATTTCAAATC ACTCGCTCAG 
TACCTGCACT CGGGCCCACC CGTGTACTTT GTCCTGGAGG AAGGCTATAA CTACAGTTCA 
CGCAAAGGGC AGAACATGGT GTGCGGCGGC ATGGGCTGTG ACAATGACTC CCTGGTGCAG 
CAGATATTTA ACGCAGCTGA GCTGGACACC TACACCCGAG TAGGCTTCGC CCCCTCGTCC 
TGGATCGATG ACTACTTTGA CTGGGTCTCG CCACAGTCGT CCTGCTGCAG ACTCTACAAC 
GTCACTCACC AGTTCTGCAA TGCTTCTGTG ATGGACCCAA CCTGTGTCCG CTGCAGACCT 
CTGACTCCAG AGGGTAAACA GAGGCCTCAG GGGAAAGAAT TCATGAAATT CCTGCCCATG 
TTCCTTTCTG ATAACCCCAA CCCCAAGTGC GGCAAAGGGG GACATGCTGC TTACGGTTCA 
GCTGTTAACA TTGTGGGAGA TGACACTTAC ATTGGGGCCA CTTACTTCAT GACCTACCAC 
ACCATACTTA AGACCTCCGC TGACTATACT GATGCCATGA AGAAAGCTCG GCTAATAGCC 
AGTAACATCA CGGAAACCAT GCGTTCTAAG GGGAGTGACT ACCGCGTATT CCCTTACAGT 
GTGTTCTACG TCTTCTATGA ACAGTACCTG ACCATTATTG ATGACACCAT CTTTAACCTC 
AGTGTGTCTC TGGGCTCCAT ATTTCTGGTG ACCTTGGTGG TTCTGGGCTG TGAGCTGTGG 
TCTGCGGTCA TCATGTGTAT CACCATAGCC ATGATCCTGG TCAACATGTT CGGTGTCATG 
TGGCTGTGGG GCATCAGTCT GAATGCGGTC TCCTTGGTCA ACTTGGTGAT GAGCTGCGGC 
ATTTCTGTGG AGTTCTGCAG CCATATAACG AGAGCATTCA CCATGAGTAC CAAAGGAAGC 
CGAGTGAGCC GGGCGGAAGA GGCACTGGCC CACATGGGTA GTTCTGTATT CAGTGGAATC 
ACACTTACGA AATTTGGAGG GATCGTGGTG TTAGCCTTTG CCAAATCTCA AATTTTTGAG 
ATATTTTACT TCAGGATGTA CTTAGCCATG GTCTTACTCG GAGCCACTCA TGGACTAATA 
TTTCTTCCCG TCTTACTCAG TTACATAGGG CCGTCGGTGA ATAAAGCTAA AAGACACACC 
ACTTACGAGC GCTACAGAGG GACAGAGAGA GAACGGCTCC TCAATTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173338.1
CDS	196..4026
Translation	MGAHHPALGLLLLLLCPAQVFSQSCVWYGECGIATGDKRYNCKYSGPPKPLPKDGYDLVQELCPGLFFDNVSLCCDIQQLQTLKSNLQLPLQFLSRCPSCFYNLMTLFCELTCSPHQSQFLNVTATEDYFDPKTQENKTNVKELEYFVGQSFANAMYNACRDVEAPSSNEKALGLLCGRDARACNATNWIEYMFNKDNGQAPFTIIPVFSDLSILGMEPMRNATKGCNESVDEVTGPCSCQDCSIVCGPKPQPPPPPMPWRIWGLDAMYVIMWVTYVAFLFVFFGALLAVWCHRRYFVSEYTPIDSNIAFSVNSSDKGEASCCDPLGAAFDDCLRRMFTKWGAFCVRNPTCIIFFSLAFITVCSSGLVFVQVTTNPVELWSAPHSQARLEKEYFDKHFGPFFRTEQLIIQAPNTSVHIYEPYPAGADVPFGPPLNKEILHQVLDLQIAIESITASYNNETVTLQDICVAPLSPYNKNCTIMSVLNYFQNSHAVLDSQVGDDFYIYADYHTHFLYCVRAPASLNDTSLLHGPCLGTFGGPVFPWLVLGGYDDQNYNNATALVITFPVNNYYNDTERLQRAWAWEKEFISFVKNYKNPNLTISFTAERSIEDELNRESNSDVFTVIISYVVMFLYISLALGHIQSCSRLLVDSKISLGIAGILIVLSSVACSLGIFSYMGMPLTLIVIEVIPFLVLAVGVDNIFILVQTYQRDERLQEETLDQQLGRILGEVAPTMFLSSFSETSAFFFGALSSMPAVHTFSLFAGMAVLIDFLLQITCFVSLLGLDIKRQEKNHLDILCCVRGADDGQGSHASESYLFRFFKNYFAPLLLKDWLRPIVVAVFVGVLSFSVAVVNKVDIGLDQSLSMPNDSYVIDYFKSLAQYLHSGPPVYFVLEEGYNYSSRKGQNMVCGGMGCDNDSLVQQIFNAAELDTYTRVGFAPSSWIDDYFDWVSPQSSCCRLYNVTHQFCNASVMDPTCVRCRPLTPEGKQRPQGKEFMKFLPMFLSDNPNPKCGKGGHAAYGSAVNIVGDDTYIGATYFMTYHTILKTSADYTDAMKKARLIASNITETMRSKGSDYRVFPYSVFYVFYEQYLTIIDDTIFNLSVSLGSIFLVTLVVLGCELWSAVIMCITIAMILVNMFGVMWLWGISLNAVSLVNLVMSCGISVEFCSHITRAFTMSTKGSRVSRAEEALAHMGSSVFSGITLTKFGGIVVLAFAKSQIFEIFYFRMYLAMVLLGATHGLIFLPVLLSYIGPSVNKAKRHTTYERYRGTERERLLNF

Target ORF information:

RefSeq Version	XM_017317849.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Niemann-Pick type C1 (Npc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317849.1

ORF Insert Sequence:

ATGGGTGCGC ACCACCCGGC CCTCGGCCTG CTGCTGCTGC TGCTGTGCCC TGCGCAGGTG 
TTTTCGCAAT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CGACTGGAGA TAAGAGGTAC 
AACTGTAAAT ATTCTGGCCC ACCAAAACCC CTCCCAAAGG ACGGCTATGA CTTAGTGCAG 
GAACTCTGTC CAGGACTCTT CTTTGACAAT GTCAGTCTCT GCTGTGACAT TCAACAGCTT 
CAGACGCTGA AGAGTAACCT GCAGCTGCCC CTGCAGTTCC TGTCCAGGTG TCCGTCATGT 
TTTTATAACC TAATGACCCT GTTTTGTGAG CTAACATGTA GCCCACACCA GAGTCAGTTT 
CTGAATGTGA CAGCAACTGA GGATTATTTT GATCCTAAGA CACAGGAGAA TAAAACAAAT 
GTAAAGGAAT TAGAGTACTT TGTCGGACAG AGCTTCGCGA ATGCGATGTA CAATGCCTGC 
CGTGATGTGG AGGCGCCTTC CAGTAACGAG AAGGCCTTAG GACTCTTGTG TGGGAGGGAT 
GCCCGTGCCT GCAATGCCAC CAACTGGATT GAGTACATGT TCAATAAAGA CAACGGACAA 
GCGCCATTTA CCATCATTCC TGTGTTTTCA GATCTTTCAA TCCTTGGGAT GGAGCCCATG 
AGAAATGCCA CCAAAGGCTG CAATGAGTCT GTAGATGAGG TCACGGGGCC ATGTAGCTGC 
CAGGACTGCT CCATCGTCTG CGGCCCCAAG CCCCAGCCCC CACCCCCTCC TATGCCCTGG 
AGGATCTGGG GCTTGGATGC CATGTATGTC ATCATGTGGG TCACCTACGT GGCATTTCTG 
TTTGTGTTTT TTGGAGCACT GTTGGCAGTG TGGTGCCACA GGCGGTACTT TGTGTCTGAG 
TACACTCCCA TTGACAGTAA CATCGCCTTT TCTGTGAATA GCAGTGACAA AGGGGAAGCC 
TCATGCTGTG ACCCACTTGG TGCAGCATTT GATGACTGTC TGAGGCGCAT GTTCACAAAG 
TGGGGGGCTT TCTGTGTCCG AAATCCCACC TGCATCATTT TCTTCTCATT GGCCTTCATC 
ACTGTGTGCT CTTCTGGCCT GGTATTTGTC CAGGTCACCA CCAATCCTGT AGAGCTCTGG 
TCAGCCCCTC ACAGTCAGGC CCGCTTGGAA AAGGAGTACT TTGACAAGCA CTTTGGGCCT 
TTCTTTCGCA CGGAGCAGCT TATCATCCAG GCCCCCAACA CCAGTGTTCA TATCTACGAA 
CCGTACCCCG CAGGAGCCGA TGTGCCCTTC GGGCCTCCAT TGAACAAAGA GATTCTGCAC 
CAGGTTCTGG ACTTACAGAT CGCCATTGAA AGCATCACCG CATCTTACAA CAATGAAACC 
GTGACACTGC AGGACATCTG TGTGGCCCCC CTCTCTCCAT ACAACAAGAA CTGCACCATT 
ATGAGTGTGT TAAATTACTT CCAGAACAGC CATGCGGTGC TGGACAGCCA AGTAGGCGAC 
GACTTCTATA TCTACGCTGA TTACCACACA CACTTTCTGT ACTGTGTACG GGCTCCCGCC 
TCCTTGAATG ATACGAGTTT GCTCCACGGT CCTTGCCTGG GTACATTTGG AGGACCGGTG 
TTCCCGTGGC TTGTGTTGGG TGGCTATGAT GATCAGAACT ACAACAATGC CACCGCGCTT 
GTGATCACCT TCCCCGTGAA TAATTACTAC AATGACACAG AGAGGCTCCA GAGGGCCTGG 
GCCTGGGAGA AAGAGTTTAT TAGTTTTGTG AAAAACTACA AGAATCCAAA TCTGACCATT 
TCTTTCACTG CTGAGCGAAG CATCGAAGAT GAGCTCAATC GGGAAAGTAA CAGTGACGTG 
TTCACCGTCA TCATCAGCTA CGTCGTGATG TTTCTGTACA TTTCCCTCGC CCTGGGTCAC 
ATCCAGAGCT GCAGCAGGCT CCTGGTGGAT TCTAAGATCT CGCTGGGCAT TGCGGGGATC 
CTGATCGTGC TAAGCTCGGT GGCCTGCTCT CTGGGCATCT TCAGCTACAT GGGGATGCCG 
CTGACCCTCA TCGTCATTGA GGTCATCCCA TTCCTGGTGC TGGCTGTCGG GGTGGACAAC 
ATCTTCATTC TAGTGCAGAC CTACCAGAGA GATGAGCGTC TTCAGGAGGA AACGCTGGAT 
CAGCAGCTGG GCAGGATCCT TGGAGAAGTG GCCCCGACCA TGTTCCTTTC ATCCTTTTCT 
GAGACCTCAG CATTTTTCTT TGGGGCGCTG TCCTCGATGC CAGCTGTGCA CACCTTCTCT 
CTGTTTGCGG GAATGGCCGT CCTCATTGAC TTCCTCCTTC AGATTACCTG CTTTGTGAGC 
CTGTTGGGGT TAGATATTAA GAGGCAAGAG AAAAACCATC TGGACATCCT GTGCTGTGTC 
AGAGGCGCTG ACGACGGACA AGGTAGCCAC GCCTCCGAAA GCTACCTGTT TCGCTTCTTC 
AAAAACTACT TTGCCCCCCT TCTGCTGAAG GACTGGCTGA GGCCAATTGT GGTAGCGGTG 
TTTGTGGGCG TTCTGTCATT CAGTGTTGCG GTGGTGAACA AAGTAGACAT CGGGTTGGAT 
CAGTCTCTCT CAATGCCAAA CGATTCGTAT GTGATTGACT ATTTCAAATC ACTCGCTCAG 
TACCTGCACT CGGGCCCACC CGTGTACTTT GTCCTGGAGG AAGGCTATAA CTACAGTTCA 
CGCAAAGGGC AGAACATGGT GTGCGGCGGC ATGGGCTGTG ACAATGACTC CCTGGTGCAG 
CAGATATTTA ACGCAGCTGA GCTGGACACC TACACCCGAG TAGGCTTCGC CCCCTCGTCC 
TGGATCGATG ACTACTTTGA CTGGGTCTCG CCACAGTCGT CCTGCTGCAG ACTCTACAAC 
GTCACTCACC AGTTCTGCAA TGCTTCTGTG ATGGACCCAA CCTGTGTCCG CTGCAGACCT 
CTGACTCCAG AGGGTAAACA GAGGCCTCAG GGGAAAGAAT TCATGAAATT CCTGCCCATG 
TTCCTTTCTG ATAACCCCAA CCCCAAGTGC GGCAAAGGGG GACATGCTGC TTACGGTTCA 
GCTGTTAACA TTGTGGGAGA TGACACTTAC ATTGGGGCCA CTTACTTCAT GACCTACCAC 
ACCATACTTA AGACCTCCGC TGACTATACT GATGCCATGA AGAAAGCTCG GCTAATAGCC 
AGTAACATCA CGGAAACCAT GCGTTCTAAG GGGAGTGACT ACCGCGTATT CCCTTACAGT 
GTGTTCTACG TCTTCTATGA ACAGTACCTG ACCATTATTG ATGACACCAT CTTTAACCTC 
AGTGTGTCTC TGGGCTCCAT ATTTCTGGTG ACCTTGGTGG TTCTGGGCTG TGAGCTGTGG 
TCTGCGGTCA TCATGTGTAT CACCATAGCC ATGATCCTGG TCAACATGTT CGGTGTCATG 
TGGCTGTGGG GCATCAGTCT GAATGCGGTC TCCTTGGTCA ACTTGGTGAT GAGCTGCGGC 
ATTTCTGTGG AGTTCTGCAG CCATATAACG AGAGCATTCA CCATGAGTAC CAAAGGAAGC 
CGAGTGAGCC GGGCGGAAGA GGCACTGGCC CACATGGGTA GTTCTGTATT CAGTGGAATC 
ACACTTACGA AATTTGGAGG GATCGTGGTG TTAGCCTTTG CCAAATCTCA AATTTTTGAG 
ATATTTTACT TCAGGATGTA CTTAGCCATG GTCTTACTCG GAGCCACTCA TGGACTAATA 
TTTCTTCCCG TCTTACTCAG TTACATAGGG CCGTCGGTGA ATAAAGCTAA AAGACACACC 
ACTTACGAGC GCTACAGAGG GACAGAGAGA GAACGGCTCC TCAATTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Npc1 ( XM_017317849.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173338.1 Mus musculus NPC intracellular cholesterol transporter 1 (Npc1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu79770
Clone ID Related Accession (Same CDS sequence)	XM_017317849.1 , XM_017317849.2
Accession Version	XM_017317849.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3831bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	NPC intracellular cholesterol transporter 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317849.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGCGC ACCACCCGGC CCTCGGCCTG CTGCTGCTGC TGCTGTGCCC TGCGCAGGTG 
TTTTCGCAAT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CGACTGGAGA TAAGAGGTAC 
AACTGTAAAT ATTCTGGCCC ACCAAAACCC CTCCCAAAGG ACGGCTATGA CTTAGTGCAG 
GAACTCTGTC CAGGACTCTT CTTTGACAAT GTCAGTCTCT GCTGTGACAT TCAACAGCTT 
CAGACGCTGA AGAGTAACCT GCAGCTGCCC CTGCAGTTCC TGTCCAGGTG TCCGTCATGT 
TTTTATAACC TAATGACCCT GTTTTGTGAG CTAACATGTA GCCCACACCA GAGTCAGTTT 
CTGAATGTGA CAGCAACTGA GGATTATTTT GATCCTAAGA CACAGGAGAA TAAAACAAAT 
GTAAAGGAAT TAGAGTACTT TGTCGGACAG AGCTTCGCGA ATGCGATGTA CAATGCCTGC 
CGTGATGTGG AGGCGCCTTC CAGTAACGAG AAGGCCTTAG GACTCTTGTG TGGGAGGGAT 
GCCCGTGCCT GCAATGCCAC CAACTGGATT GAGTACATGT TCAATAAAGA CAACGGACAA 
GCGCCATTTA CCATCATTCC TGTGTTTTCA GATCTTTCAA TCCTTGGGAT GGAGCCCATG 
AGAAATGCCA CCAAAGGCTG CAATGAGTCT GTAGATGAGG TCACGGGGCC ATGTAGCTGC 
CAGGACTGCT CCATCGTCTG CGGCCCCAAG CCCCAGCCCC CACCCCCTCC TATGCCCTGG 
AGGATCTGGG GCTTGGATGC CATGTATGTC ATCATGTGGG TCACCTACGT GGCATTTCTG 
TTTGTGTTTT TTGGAGCACT GTTGGCAGTG TGGTGCCACA GGCGGTACTT TGTGTCTGAG 
TACACTCCCA TTGACAGTAA CATCGCCTTT TCTGTGAATA GCAGTGACAA AGGGGAAGCC 
TCATGCTGTG ACCCACTTGG TGCAGCATTT GATGACTGTC TGAGGCGCAT GTTCACAAAG 
TGGGGGGCTT TCTGTGTCCG AAATCCCACC TGCATCATTT TCTTCTCATT GGCCTTCATC 
ACTGTGTGCT CTTCTGGCCT GGTATTTGTC CAGGTCACCA CCAATCCTGT AGAGCTCTGG 
TCAGCCCCTC ACAGTCAGGC CCGCTTGGAA AAGGAGTACT TTGACAAGCA CTTTGGGCCT 
TTCTTTCGCA CGGAGCAGCT TATCATCCAG GCCCCCAACA CCAGTGTTCA TATCTACGAA 
CCGTACCCCG CAGGAGCCGA TGTGCCCTTC GGGCCTCCAT TGAACAAAGA GATTCTGCAC 
CAGGTTCTGG ACTTACAGAT CGCCATTGAA AGCATCACCG CATCTTACAA CAATGAAACC 
GTGACACTGC AGGACATCTG TGTGGCCCCC CTCTCTCCAT ACAACAAGAA CTGCACCATT 
ATGAGTGTGT TAAATTACTT CCAGAACAGC CATGCGGTGC TGGACAGCCA AGTAGGCGAC 
GACTTCTATA TCTACGCTGA TTACCACACA CACTTTCTGT ACTGTGTACG GGCTCCCGCC 
TCCTTGAATG ATACGAGTTT GCTCCACGGT CCTTGCCTGG GTACATTTGG AGGACCGGTG 
TTCCCGTGGC TTGTGTTGGG TGGCTATGAT GATCAGAACT ACAACAATGC CACCGCGCTT 
GTGATCACCT TCCCCGTGAA TAATTACTAC AATGACACAG AGAGGCTCCA GAGGGCCTGG 
GCCTGGGAGA AAGAGTTTAT TAGTTTTGTG AAAAACTACA AGAATCCAAA TCTGACCATT 
TCTTTCACTG CTGAGCGAAG CATCGAAGAT GAGCTCAATC GGGAAAGTAA CAGTGACGTG 
TTCACCGTCA TCATCAGCTA CGTCGTGATG TTTCTGTACA TTTCCCTCGC CCTGGGTCAC 
ATCCAGAGCT GCAGCAGGCT CCTGGTGGAT TCTAAGATCT CGCTGGGCAT TGCGGGGATC 
CTGATCGTGC TAAGCTCGGT GGCCTGCTCT CTGGGCATCT TCAGCTACAT GGGGATGCCG 
CTGACCCTCA TCGTCATTGA GGTCATCCCA TTCCTGGTGC TGGCTGTCGG GGTGGACAAC 
ATCTTCATTC TAGTGCAGAC CTACCAGAGA GATGAGCGTC TTCAGGAGGA AACGCTGGAT 
CAGCAGCTGG GCAGGATCCT TGGAGAAGTG GCCCCGACCA TGTTCCTTTC ATCCTTTTCT 
GAGACCTCAG CATTTTTCTT TGGGGCGCTG TCCTCGATGC CAGCTGTGCA CACCTTCTCT 
CTGTTTGCGG GAATGGCCGT CCTCATTGAC TTCCTCCTTC AGATTACCTG CTTTGTGAGC 
CTGTTGGGGT TAGATATTAA GAGGCAAGAG AAAAACCATC TGGACATCCT GTGCTGTGTC 
AGAGGCGCTG ACGACGGACA AGGTAGCCAC GCCTCCGAAA GCTACCTGTT TCGCTTCTTC 
AAAAACTACT TTGCCCCCCT TCTGCTGAAG GACTGGCTGA GGCCAATTGT GGTAGCGGTG 
TTTGTGGGCG TTCTGTCATT CAGTGTTGCG GTGGTGAACA AAGTAGACAT CGGGTTGGAT 
CAGTCTCTCT CAATGCCAAA CGATTCGTAT GTGATTGACT ATTTCAAATC ACTCGCTCAG 
TACCTGCACT CGGGCCCACC CGTGTACTTT GTCCTGGAGG AAGGCTATAA CTACAGTTCA 
CGCAAAGGGC AGAACATGGT GTGCGGCGGC ATGGGCTGTG ACAATGACTC CCTGGTGCAG 
CAGATATTTA ACGCAGCTGA GCTGGACACC TACACCCGAG TAGGCTTCGC CCCCTCGTCC 
TGGATCGATG ACTACTTTGA CTGGGTCTCG CCACAGTCGT CCTGCTGCAG ACTCTACAAC 
GTCACTCACC AGTTCTGCAA TGCTTCTGTG ATGGACCCAA CCTGTGTCCG CTGCAGACCT 
CTGACTCCAG AGGGTAAACA GAGGCCTCAG GGGAAAGAAT TCATGAAATT CCTGCCCATG 
TTCCTTTCTG ATAACCCCAA CCCCAAGTGC GGCAAAGGGG GACATGCTGC TTACGGTTCA 
GCTGTTAACA TTGTGGGAGA TGACACTTAC ATTGGGGCCA CTTACTTCAT GACCTACCAC 
ACCATACTTA AGACCTCCGC TGACTATACT GATGCCATGA AGAAAGCTCG GCTAATAGCC 
AGTAACATCA CGGAAACCAT GCGTTCTAAG GGGAGTGACT ACCGCGTATT CCCTTACAGT 
GTGTTCTACG TCTTCTATGA ACAGTACCTG ACCATTATTG ATGACACCAT CTTTAACCTC 
AGTGTGTCTC TGGGCTCCAT ATTTCTGGTG ACCTTGGTGG TTCTGGGCTG TGAGCTGTGG 
TCTGCGGTCA TCATGTGTAT CACCATAGCC ATGATCCTGG TCAACATGTT CGGTGTCATG 
TGGCTGTGGG GCATCAGTCT GAATGCGGTC TCCTTGGTCA ACTTGGTGAT GAGCTGCGGC 
ATTTCTGTGG AGTTCTGCAG CCATATAACG AGAGCATTCA CCATGAGTAC CAAAGGAAGC 
CGAGTGAGCC GGGCGGAAGA GGCACTGGCC CACATGGGTA GTTCTGTATT CAGTGGAATC 
ACACTTACGA AATTTGGAGG GATCGTGGTG TTAGCCTTTG CCAAATCTCA AATTTTTGAG 
ATATTTTACT TCAGGATGTA CTTAGCCATG GTCTTACTCG GAGCCACTCA TGGACTAATA 
TTTCTTCCCG TCTTACTCAG TTACATAGGG CCGTCGGTGA ATAAAGCTAA AAGACACACC 
ACTTACGAGC GCTACAGAGG GACAGAGAGA GAACGGCTCC TCAATTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173338.1
CDS	270..4100
Translation	MGAHHPALGLLLLLLCPAQVFSQSCVWYGECGIATGDKRYNCKYSGPPKPLPKDGYDLVQELCPGLFFDNVSLCCDIQQLQTLKSNLQLPLQFLSRCPSCFYNLMTLFCELTCSPHQSQFLNVTATEDYFDPKTQENKTNVKELEYFVGQSFANAMYNACRDVEAPSSNEKALGLLCGRDARACNATNWIEYMFNKDNGQAPFTIIPVFSDLSILGMEPMRNATKGCNESVDEVTGPCSCQDCSIVCGPKPQPPPPPMPWRIWGLDAMYVIMWVTYVAFLFVFFGALLAVWCHRRYFVSEYTPIDSNIAFSVNSSDKGEASCCDPLGAAFDDCLRRMFTKWGAFCVRNPTCIIFFSLAFITVCSSGLVFVQVTTNPVELWSAPHSQARLEKEYFDKHFGPFFRTEQLIIQAPNTSVHIYEPYPAGADVPFGPPLNKEILHQVLDLQIAIESITASYNNETVTLQDICVAPLSPYNKNCTIMSVLNYFQNSHAVLDSQVGDDFYIYADYHTHFLYCVRAPASLNDTSLLHGPCLGTFGGPVFPWLVLGGYDDQNYNNATALVITFPVNNYYNDTERLQRAWAWEKEFISFVKNYKNPNLTISFTAERSIEDELNRESNSDVFTVIISYVVMFLYISLALGHIQSCSRLLVDSKISLGIAGILIVLSSVACSLGIFSYMGMPLTLIVIEVIPFLVLAVGVDNIFILVQTYQRDERLQEETLDQQLGRILGEVAPTMFLSSFSETSAFFFGALSSMPAVHTFSLFAGMAVLIDFLLQITCFVSLLGLDIKRQEKNHLDILCCVRGADDGQGSHASESYLFRFFKNYFAPLLLKDWLRPIVVAVFVGVLSFSVAVVNKVDIGLDQSLSMPNDSYVIDYFKSLAQYLHSGPPVYFVLEEGYNYSSRKGQNMVCGGMGCDNDSLVQQIFNAAELDTYTRVGFAPSSWIDDYFDWVSPQSSCCRLYNVTHQFCNASVMDPTCVRCRPLTPEGKQRPQGKEFMKFLPMFLSDNPNPKCGKGGHAAYGSAVNIVGDDTYIGATYFMTYHTILKTSADYTDAMKKARLIASNITETMRSKGSDYRVFPYSVFYVFYEQYLTIIDDTIFNLSVSLGSIFLVTLVVLGCELWSAVIMCITIAMILVNMFGVMWLWGISLNAVSLVNLVMSCGISVEFCSHITRAFTMSTKGSRVSRAEEALAHMGSSVFSGITLTKFGGIVVLAFAKSQIFEIFYFRMYLAMVLLGATHGLIFLPVLLSYIGPSVNKAKRHTTYERYRGTERERLLNF

Target ORF information:

RefSeq Version	XM_017317849.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus NPC intracellular cholesterol transporter 1 (Npc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317849.2

ORF Insert Sequence:

ATGGGTGCGC ACCACCCGGC CCTCGGCCTG CTGCTGCTGC TGCTGTGCCC TGCGCAGGTG 
TTTTCGCAAT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CGACTGGAGA TAAGAGGTAC 
AACTGTAAAT ATTCTGGCCC ACCAAAACCC CTCCCAAAGG ACGGCTATGA CTTAGTGCAG 
GAACTCTGTC CAGGACTCTT CTTTGACAAT GTCAGTCTCT GCTGTGACAT TCAACAGCTT 
CAGACGCTGA AGAGTAACCT GCAGCTGCCC CTGCAGTTCC TGTCCAGGTG TCCGTCATGT 
TTTTATAACC TAATGACCCT GTTTTGTGAG CTAACATGTA GCCCACACCA GAGTCAGTTT 
CTGAATGTGA CAGCAACTGA GGATTATTTT GATCCTAAGA CACAGGAGAA TAAAACAAAT 
GTAAAGGAAT TAGAGTACTT TGTCGGACAG AGCTTCGCGA ATGCGATGTA CAATGCCTGC 
CGTGATGTGG AGGCGCCTTC CAGTAACGAG AAGGCCTTAG GACTCTTGTG TGGGAGGGAT 
GCCCGTGCCT GCAATGCCAC CAACTGGATT GAGTACATGT TCAATAAAGA CAACGGACAA 
GCGCCATTTA CCATCATTCC TGTGTTTTCA GATCTTTCAA TCCTTGGGAT GGAGCCCATG 
AGAAATGCCA CCAAAGGCTG CAATGAGTCT GTAGATGAGG TCACGGGGCC ATGTAGCTGC 
CAGGACTGCT CCATCGTCTG CGGCCCCAAG CCCCAGCCCC CACCCCCTCC TATGCCCTGG 
AGGATCTGGG GCTTGGATGC CATGTATGTC ATCATGTGGG TCACCTACGT GGCATTTCTG 
TTTGTGTTTT TTGGAGCACT GTTGGCAGTG TGGTGCCACA GGCGGTACTT TGTGTCTGAG 
TACACTCCCA TTGACAGTAA CATCGCCTTT TCTGTGAATA GCAGTGACAA AGGGGAAGCC 
TCATGCTGTG ACCCACTTGG TGCAGCATTT GATGACTGTC TGAGGCGCAT GTTCACAAAG 
TGGGGGGCTT TCTGTGTCCG AAATCCCACC TGCATCATTT TCTTCTCATT GGCCTTCATC 
ACTGTGTGCT CTTCTGGCCT GGTATTTGTC CAGGTCACCA CCAATCCTGT AGAGCTCTGG 
TCAGCCCCTC ACAGTCAGGC CCGCTTGGAA AAGGAGTACT TTGACAAGCA CTTTGGGCCT 
TTCTTTCGCA CGGAGCAGCT TATCATCCAG GCCCCCAACA CCAGTGTTCA TATCTACGAA 
CCGTACCCCG CAGGAGCCGA TGTGCCCTTC GGGCCTCCAT TGAACAAAGA GATTCTGCAC 
CAGGTTCTGG ACTTACAGAT CGCCATTGAA AGCATCACCG CATCTTACAA CAATGAAACC 
GTGACACTGC AGGACATCTG TGTGGCCCCC CTCTCTCCAT ACAACAAGAA CTGCACCATT 
ATGAGTGTGT TAAATTACTT CCAGAACAGC CATGCGGTGC TGGACAGCCA AGTAGGCGAC 
GACTTCTATA TCTACGCTGA TTACCACACA CACTTTCTGT ACTGTGTACG GGCTCCCGCC 
TCCTTGAATG ATACGAGTTT GCTCCACGGT CCTTGCCTGG GTACATTTGG AGGACCGGTG 
TTCCCGTGGC TTGTGTTGGG TGGCTATGAT GATCAGAACT ACAACAATGC CACCGCGCTT 
GTGATCACCT TCCCCGTGAA TAATTACTAC AATGACACAG AGAGGCTCCA GAGGGCCTGG 
GCCTGGGAGA AAGAGTTTAT TAGTTTTGTG AAAAACTACA AGAATCCAAA TCTGACCATT 
TCTTTCACTG CTGAGCGAAG CATCGAAGAT GAGCTCAATC GGGAAAGTAA CAGTGACGTG 
TTCACCGTCA TCATCAGCTA CGTCGTGATG TTTCTGTACA TTTCCCTCGC CCTGGGTCAC 
ATCCAGAGCT GCAGCAGGCT CCTGGTGGAT TCTAAGATCT CGCTGGGCAT TGCGGGGATC 
CTGATCGTGC TAAGCTCGGT GGCCTGCTCT CTGGGCATCT TCAGCTACAT GGGGATGCCG 
CTGACCCTCA TCGTCATTGA GGTCATCCCA TTCCTGGTGC TGGCTGTCGG GGTGGACAAC 
ATCTTCATTC TAGTGCAGAC CTACCAGAGA GATGAGCGTC TTCAGGAGGA AACGCTGGAT 
CAGCAGCTGG GCAGGATCCT TGGAGAAGTG GCCCCGACCA TGTTCCTTTC ATCCTTTTCT 
GAGACCTCAG CATTTTTCTT TGGGGCGCTG TCCTCGATGC CAGCTGTGCA CACCTTCTCT 
CTGTTTGCGG GAATGGCCGT CCTCATTGAC TTCCTCCTTC AGATTACCTG CTTTGTGAGC 
CTGTTGGGGT TAGATATTAA GAGGCAAGAG AAAAACCATC TGGACATCCT GTGCTGTGTC 
AGAGGCGCTG ACGACGGACA AGGTAGCCAC GCCTCCGAAA GCTACCTGTT TCGCTTCTTC 
AAAAACTACT TTGCCCCCCT TCTGCTGAAG GACTGGCTGA GGCCAATTGT GGTAGCGGTG 
TTTGTGGGCG TTCTGTCATT CAGTGTTGCG GTGGTGAACA AAGTAGACAT CGGGTTGGAT 
CAGTCTCTCT CAATGCCAAA CGATTCGTAT GTGATTGACT ATTTCAAATC ACTCGCTCAG 
TACCTGCACT CGGGCCCACC CGTGTACTTT GTCCTGGAGG AAGGCTATAA CTACAGTTCA 
CGCAAAGGGC AGAACATGGT GTGCGGCGGC ATGGGCTGTG ACAATGACTC CCTGGTGCAG 
CAGATATTTA ACGCAGCTGA GCTGGACACC TACACCCGAG TAGGCTTCGC CCCCTCGTCC 
TGGATCGATG ACTACTTTGA CTGGGTCTCG CCACAGTCGT CCTGCTGCAG ACTCTACAAC 
GTCACTCACC AGTTCTGCAA TGCTTCTGTG ATGGACCCAA CCTGTGTCCG CTGCAGACCT 
CTGACTCCAG AGGGTAAACA GAGGCCTCAG GGGAAAGAAT TCATGAAATT CCTGCCCATG 
TTCCTTTCTG ATAACCCCAA CCCCAAGTGC GGCAAAGGGG GACATGCTGC TTACGGTTCA 
GCTGTTAACA TTGTGGGAGA TGACACTTAC ATTGGGGCCA CTTACTTCAT GACCTACCAC 
ACCATACTTA AGACCTCCGC TGACTATACT GATGCCATGA AGAAAGCTCG GCTAATAGCC 
AGTAACATCA CGGAAACCAT GCGTTCTAAG GGGAGTGACT ACCGCGTATT CCCTTACAGT 
GTGTTCTACG TCTTCTATGA ACAGTACCTG ACCATTATTG ATGACACCAT CTTTAACCTC 
AGTGTGTCTC TGGGCTCCAT ATTTCTGGTG ACCTTGGTGG TTCTGGGCTG TGAGCTGTGG 
TCTGCGGTCA TCATGTGTAT CACCATAGCC ATGATCCTGG TCAACATGTT CGGTGTCATG 
TGGCTGTGGG GCATCAGTCT GAATGCGGTC TCCTTGGTCA ACTTGGTGAT GAGCTGCGGC 
ATTTCTGTGG AGTTCTGCAG CCATATAACG AGAGCATTCA CCATGAGTAC CAAAGGAAGC 
CGAGTGAGCC GGGCGGAAGA GGCACTGGCC CACATGGGTA GTTCTGTATT CAGTGGAATC 
ACACTTACGA AATTTGGAGG GATCGTGGTG TTAGCCTTTG CCAAATCTCA AATTTTTGAG 
ATATTTTACT TCAGGATGTA CTTAGCCATG GTCTTACTCG GAGCCACTCA TGGACTAATA 
TTTCTTCCCG TCTTACTCAG TTACATAGGG CCGTCGGTGA ATAAAGCTAA AAGACACACC 
ACTTACGAGC GCTACAGAGG GACAGAGAGA GAACGGCTCC TCAATTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Npc1 ( NM_008720.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032746.2 Mus musculus NPC intracellular cholesterol transporter 1 (Npc1), mRNA.

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CloneID	OMu21708
Clone ID Related Accession (Same CDS sequence)	NM_008720.2
Accession Version	NM_008720.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	NPC intracellular cholesterol transporter 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC052437.1, CF585180.1 and AK003935.1. On Mar 7, 2006 this sequence version replaced NM_008720.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC052437.1, AF003348.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGGTGCGC ACCACCCGGC CCTCGGCCTG CTGCTGCTGC TGCTGTGCCC TGCGCAGGTG 
TTTTCGCAAT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CGACTGGAGA TAAGAGGTAC 
AACTGTAAAT ATTCTGGCCC ACCAAAACCC CTCCCAAAGG ACGGCTATGA CTTAGTGCAG 
GAACTCTGTC CAGGACTCTT CTTTGACAAT GTCAGTCTCT GCTGTGACAT TCAACAGCTT 
CAGACGCTGA AGAGTAACCT GCAGCTGCCC CTGCAGTTCC TGTCCAGGTG TCCGTCATGT 
TTTTATAACC TAATGACCCT GTTTTGTGAG CTAACATGTA GCCCACACCA GAGTCAGTTT 
CTGAATGTGA CAGCAACTGA GGATTATTTT GATCCTAAGA CACAGGAGAA TAAAACAAAT 
GTAAAGGAAT TAGAGTACTT TGTCGGACAG AGCTTCGCGA ATGCGATGTA CAATGCCTGC 
CGTGATGTGG AGGCGCCTTC CAGTAACGAG AAGGCCTTAG GACTCTTGTG TGGGAGGGAT 
GCCCGTGCCT GCAATGCCAC CAACTGGATT GAGTACATGT TCAATAAAGA CAACGGACAA 
GCGCCATTTA CCATCATTCC TGTGTTTTCA GATCTTTCAA TCCTTGGGAT GGAGCCCATG 
AGAAATGCCA CCAAAGGCTG CAATGAGTCT GTAGATGAGG TCACGGGGCC ATGTAGCTGC 
CAGGACTGCT CCATCGTCTG CGGCCCCAAG CCCCAGCCCC CACCCCCTCC TATGCCCTGG 
AGGATCTGGG GCTTGGATGC CATGTATGTC ATCATGTGGG TCACCTACGT GGCATTTCTG 
TTTGTGTTTT TTGGAGCACT GTTGGCAGTG TGGTGCCACA GAAGGCGGTA CTTTGTGTCT 
GAGTACACTC CCATTGACAG TAACATCGCC TTTTCTGTGA ATAGCAGTGA CAAAGGGGAA 
GCCTCATGCT GTGACCCACT TGGTGCAGCA TTTGATGACT GTCTGAGGCG CATGTTCACA 
AAGTGGGGGG CTTTCTGTGT CCGAAATCCC ACCTGCATCA TTTTCTTCTC ATTGGCCTTC 
ATCACTGTGT GCTCTTCTGG CCTGGTATTT GTCCAGGTCA CCACCAATCC TGTAGAGCTC 
TGGTCAGCCC CTCACAGTCA GGCCCGCTTG GAAAAGGAGT ACTTTGACAA GCACTTTGGG 
CCTTTCTTTC GCACGGAGCA GCTTATCATC CAGGCCCCCA ACACCAGTGT TCATATCTAC 
GAACCGTACC CCGCAGGAGC CGATGTGCCC TTCGGGCCTC CATTGAACAA AGAGATTCTG 
CACCAGGTTC TGGACTTACA GATCGCCATT GAAAGCATCA CCGCATCTTA CAACAATGAA 
ACCGTGACAC TGCAGGACAT CTGTGTGGCC CCCCTCTCTC CATACAACAA GAACTGCACC 
ATTATGAGTG TGTTAAATTA CTTCCAGAAC AGCCATGCGG TGCTGGACAG CCAAGTAGGC 
GACGACTTCT ATATCTACGC TGATTACCAC ACACACTTTC TGTACTGTGT ACGGGCTCCC 
GCCTCCTTGA ATGATACGAG TTTGCTCCAC GGTCCTTGCC TGGGTACATT TGGAGGACCG 
GTGTTCCCGT GGCTTGTGTT GGGTGGCTAT GATGATCAGA ACTACAACAA TGCCACCGCG 
CTTGTGATCA CCTTCCCCGT GAATAATTAC TACAATGACA CAGAGAGGCT CCAGAGGGCC 
TGGGCCTGGG AGAAAGAGTT TATTAGTTTT GTGAAAAACT ACAAGAATCC AAATCTGACC 
ATTTCTTTCA CTGCTGAGCG AAGCATCGAA GATGAGCTCA ATCGGGAAAG TAACAGTGAC 
GTGTTCACCG TCATCATCAG CTACGTCGTG ATGTTTCTGT ACATTTCCCT CGCCCTGGGT 
CACATCCAGA GCTGCAGCAG GCTCCTGGTG GATTCTAAGA TCTCGCTGGG CATTGCGGGG 
ATCCTGATCG TGCTAAGCTC GGTGGCCTGC TCTCTGGGCA TCTTCAGCTA CATGGGGATG 
CCGCTGACCC TCATCGTCAT TGAGGTCATC CCATTCCTGG TGCTGGCTGT CGGGGTGGAC 
AACATCTTCA TTCTAGTGCA GACCTACCAG AGAGATGAGC GTCTTCAGGA GGAAACGCTG 
GATCAGCAGC TGGGCAGGAT CCTTGGAGAA GTGGCCCCGA CCATGTTCCT TTCATCCTTT 
TCTGAGACCT CAGCATTTTT CTTTGGGGCG CTGTCCTCGA TGCCAGCTGT GCACACCTTC 
TCTCTGTTTG CGGGAATGGC CGTCCTCATT GACTTCCTCC TTCAGATTAC CTGCTTTGTG 
AGCCTGTTGG GGTTAGATAT TAAGAGGCAA GAGAAAAACC ATCTGGACAT CCTGTGCTGT 
GTCAGAGGCG CTGACGACGG ACAAGGTAGC CACGCCTCCG AAAGCTACCT GTTTCGCTTC 
TTCAAAAACT ACTTTGCCCC CCTTCTGCTG AAGGACTGGC TGAGGCCAAT TGTGGTAGCG 
GTGTTTGTGG GCGTTCTGTC ATTCAGTGTT GCGGTGGTGA ACAAAGTAGA CATCGGGTTG 
GATCAGTCTC TCTCAATGCC AAACGATTCG TATGTGATTG ACTATTTCAA ATCACTCGCT 
CAGTACCTGC ACTCGGGCCC ACCCGTGTAC TTTGTCCTGG AGGAAGGCTA TAACTACAGT 
TCACGCAAAG GGCAGAACAT GGTGTGCGGC GGCATGGGCT GTGACAATGA CTCCCTGGTG 
CAGCAGATAT TTAACGCAGC TGAGCTGGAC ACCTACACCC GAGTAGGCTT CGCCCCCTCG 
TCCTGGATCG ATGACTACTT TGACTGGGTC TCGCCACAGT CGTCCTGCTG CAGACTCTAC 
AACGTCACTC ACCAGTTCTG CAATGCTTCT GTGATGGACC CAACCTGTGT CCGCTGCAGA 
CCTCTGACTC CAGAGGGTAA ACAGAGGCCT CAGGGGAAAG AATTCATGAA ATTCCTGCCC 
ATGTTCCTTT CTGATAACCC CAACCCCAAG TGCGGCAAAG GGGGACATGC TGCTTACGGT 
TCAGCTGTTA ACATTGTGGG AGATGACACT TACATTGGGG CCACTTACTT CATGACCTAC 
CACACCATAC TTAAGACCTC CGCTGACTAT ACTGATGCCA TGAAGAAAGC TCGGCTAATA 
GCCAGTAACA TCACGGAAAC CATGCGTTCT AAGGGGAGTG ACTACCGCGT ATTCCCTTAC 
AGTGTGTTCT ACGTCTTCTA TGAACAGTAC CTGACCATTA TTGATGACAC CATCTTTAAC 
CTCAGTGTGT CTCTGGGCTC CATATTTCTG GTGACCTTGG TGGTTCTGGG CTGTGAGCTG 
TGGTCTGCGG TCATCATGTG TATCACCATA GCCATGATCC TGGTCAACAT GTTCGGTGTC 
ATGTGGCTGT GGGGCATCAG TCTGAATGCG GTCTCCTTGG TCAACTTGGT GATGAGCTGC 
GGCATTTCTG TGGAGTTCTG CAGCCATATA ACGAGAGCAT TCACCATGAG TACCAAAGGA 
AGCCGAGTGA GCCGGGCGGA AGAGGCACTG GCCCACATGG GTAGTTCTGT ATTCAGTGGA 
ATCACACTTA CGAAATTTGG AGGGATCGTG GTGTTAGCCT TTGCCAAATC TCAAATTTTT 
GAGATATTTT ACTTCAGGAT GTACTTAGCC ATGGTCTTAC TCGGAGCCAC TCATGGACTA 
ATATTTCTTC CCGTCTTACT CAGTTACATA GGGCCGTCGG TGAATAAAGC TAAAAGACAC 
ACCACTTACG AGCGCTACAG AGGGACAGAG AGAGAACGGC TCCTCAATTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032746.2
CDS	150..3983
Translation	MGAHHPALGLLLLLLCPAQVFSQSCVWYGECGIATGDKRYNCKYSGPPKPLPKDGYDLVQELCPGLFFDNVSLCCDIQQLQTLKSNLQLPLQFLSRCPSCFYNLMTLFCELTCSPHQSQFLNVTATEDYFDPKTQENKTNVKELEYFVGQSFANAMYNACRDVEAPSSNEKALGLLCGRDARACNATNWIEYMFNKDNGQAPFTIIPVFSDLSILGMEPMRNATKGCNESVDEVTGPCSCQDCSIVCGPKPQPPPPPMPWRIWGLDAMYVIMWVTYVAFLFVFFGALLAVWCHRRRYFVSEYTPIDSNIAFSVNSSDKGEASCCDPLGAAFDDCLRRMFTKWGAFCVRNPTCIIFFSLAFITVCSSGLVFVQVTTNPVELWSAPHSQARLEKEYFDKHFGPFFRTEQLIIQAPNTSVHIYEPYPAGADVPFGPPLNKEILHQVLDLQIAIESITASYNNETVTLQDICVAPLSPYNKNCTIMSVLNYFQNSHAVLDSQVGDDFYIYADYHTHFLYCVRAPASLNDTSLLHGPCLGTFGGPVFPWLVLGGYDDQNYNNATALVITFPVNNYYNDTERLQRAWAWEKEFISFVKNYKNPNLTISFTAERSIEDELNRESNSDVFTVIISYVVMFLYISLALGHIQSCSRLLVDSKISLGIAGILIVLSSVACSLGIFSYMGMPLTLIVIEVIPFLVLAVGVDNIFILVQTYQRDERLQEETLDQQLGRILGEVAPTMFLSSFSETSAFFFGALSSMPAVHTFSLFAGMAVLIDFLLQITCFVSLLGLDIKRQEKNHLDILCCVRGADDGQGSHASESYLFRFFKNYFAPLLLKDWLRPIVVAVFVGVLSFSVAVVNKVDIGLDQSLSMPNDSYVIDYFKSLAQYLHSGPPVYFVLEEGYNYSSRKGQNMVCGGMGCDNDSLVQQIFNAAELDTYTRVGFAPSSWIDDYFDWVSPQSSCCRLYNVTHQFCNASVMDPTCVRCRPLTPEGKQRPQGKEFMKFLPMFLSDNPNPKCGKGGHAAYGSAVNIVGDDTYIGATYFMTYHTILKTSADYTDAMKKARLIASNITETMRSKGSDYRVFPYSVFYVFYEQYLTIIDDTIFNLSVSLGSIFLVTLVVLGCELWSAVIMCITIAMILVNMFGVMWLWGISLNAVSLVNLVMSCGISVEFCSHITRAFTMSTKGSRVSRAEEALAHMGSSVFSGITLTKFGGIVVLAFAKSQIFEIFYFRMYLAMVLLGATHGLIFLPVLLSYIGPSVNKAKRHTTYERYRGTERERLLNF

Target ORF information:

RefSeq Version	NM_008720.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus NPC intracellular cholesterol transporter 1 (Npc1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008720.2

ORF Insert Sequence:

ATGGGTGCGC ACCACCCGGC CCTCGGCCTG CTGCTGCTGC TGCTGTGCCC TGCGCAGGTG 
TTTTCGCAAT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CGACTGGAGA TAAGAGGTAC 
AACTGTAAAT ATTCTGGCCC ACCAAAACCC CTCCCAAAGG ACGGCTATGA CTTAGTGCAG 
GAACTCTGTC CAGGACTCTT CTTTGACAAT GTCAGTCTCT GCTGTGACAT TCAACAGCTT 
CAGACGCTGA AGAGTAACCT GCAGCTGCCC CTGCAGTTCC TGTCCAGGTG TCCGTCATGT 
TTTTATAACC TAATGACCCT GTTTTGTGAG CTAACATGTA GCCCACACCA GAGTCAGTTT 
CTGAATGTGA CAGCAACTGA GGATTATTTT GATCCTAAGA CACAGGAGAA TAAAACAAAT 
GTAAAGGAAT TAGAGTACTT TGTCGGACAG AGCTTCGCGA ATGCGATGTA CAATGCCTGC 
CGTGATGTGG AGGCGCCTTC CAGTAACGAG AAGGCCTTAG GACTCTTGTG TGGGAGGGAT 
GCCCGTGCCT GCAATGCCAC CAACTGGATT GAGTACATGT TCAATAAAGA CAACGGACAA 
GCGCCATTTA CCATCATTCC TGTGTTTTCA GATCTTTCAA TCCTTGGGAT GGAGCCCATG 
AGAAATGCCA CCAAAGGCTG CAATGAGTCT GTAGATGAGG TCACGGGGCC ATGTAGCTGC 
CAGGACTGCT CCATCGTCTG CGGCCCCAAG CCCCAGCCCC CACCCCCTCC TATGCCCTGG 
AGGATCTGGG GCTTGGATGC CATGTATGTC ATCATGTGGG TCACCTACGT GGCATTTCTG 
TTTGTGTTTT TTGGAGCACT GTTGGCAGTG TGGTGCCACA GAAGGCGGTA CTTTGTGTCT 
GAGTACACTC CCATTGACAG TAACATCGCC TTTTCTGTGA ATAGCAGTGA CAAAGGGGAA 
GCCTCATGCT GTGACCCACT TGGTGCAGCA TTTGATGACT GTCTGAGGCG CATGTTCACA 
AAGTGGGGGG CTTTCTGTGT CCGAAATCCC ACCTGCATCA TTTTCTTCTC ATTGGCCTTC 
ATCACTGTGT GCTCTTCTGG CCTGGTATTT GTCCAGGTCA CCACCAATCC TGTAGAGCTC 
TGGTCAGCCC CTCACAGTCA GGCCCGCTTG GAAAAGGAGT ACTTTGACAA GCACTTTGGG 
CCTTTCTTTC GCACGGAGCA GCTTATCATC CAGGCCCCCA ACACCAGTGT TCATATCTAC 
GAACCGTACC CCGCAGGAGC CGATGTGCCC TTCGGGCCTC CATTGAACAA AGAGATTCTG 
CACCAGGTTC TGGACTTACA GATCGCCATT GAAAGCATCA CCGCATCTTA CAACAATGAA 
ACCGTGACAC TGCAGGACAT CTGTGTGGCC CCCCTCTCTC CATACAACAA GAACTGCACC 
ATTATGAGTG TGTTAAATTA CTTCCAGAAC AGCCATGCGG TGCTGGACAG CCAAGTAGGC 
GACGACTTCT ATATCTACGC TGATTACCAC ACACACTTTC TGTACTGTGT ACGGGCTCCC 
GCCTCCTTGA ATGATACGAG TTTGCTCCAC GGTCCTTGCC TGGGTACATT TGGAGGACCG 
GTGTTCCCGT GGCTTGTGTT GGGTGGCTAT GATGATCAGA ACTACAACAA TGCCACCGCG 
CTTGTGATCA CCTTCCCCGT GAATAATTAC TACAATGACA CAGAGAGGCT CCAGAGGGCC 
TGGGCCTGGG AGAAAGAGTT TATTAGTTTT GTGAAAAACT ACAAGAATCC AAATCTGACC 
ATTTCTTTCA CTGCTGAGCG AAGCATCGAA GATGAGCTCA ATCGGGAAAG TAACAGTGAC 
GTGTTCACCG TCATCATCAG CTACGTCGTG ATGTTTCTGT ACATTTCCCT CGCCCTGGGT 
CACATCCAGA GCTGCAGCAG GCTCCTGGTG GATTCTAAGA TCTCGCTGGG CATTGCGGGG 
ATCCTGATCG TGCTAAGCTC GGTGGCCTGC TCTCTGGGCA TCTTCAGCTA CATGGGGATG 
CCGCTGACCC TCATCGTCAT TGAGGTCATC CCATTCCTGG TGCTGGCTGT CGGGGTGGAC 
AACATCTTCA TTCTAGTGCA GACCTACCAG AGAGATGAGC GTCTTCAGGA GGAAACGCTG 
GATCAGCAGC TGGGCAGGAT CCTTGGAGAA GTGGCCCCGA CCATGTTCCT TTCATCCTTT 
TCTGAGACCT CAGCATTTTT CTTTGGGGCG CTGTCCTCGA TGCCAGCTGT GCACACCTTC 
TCTCTGTTTG CGGGAATGGC CGTCCTCATT GACTTCCTCC TTCAGATTAC CTGCTTTGTG 
AGCCTGTTGG GGTTAGATAT TAAGAGGCAA GAGAAAAACC ATCTGGACAT CCTGTGCTGT 
GTCAGAGGCG CTGACGACGG ACAAGGTAGC CACGCCTCCG AAAGCTACCT GTTTCGCTTC 
TTCAAAAACT ACTTTGCCCC CCTTCTGCTG AAGGACTGGC TGAGGCCAAT TGTGGTAGCG 
GTGTTTGTGG GCGTTCTGTC ATTCAGTGTT GCGGTGGTGA ACAAAGTAGA CATCGGGTTG 
GATCAGTCTC TCTCAATGCC AAACGATTCG TATGTGATTG ACTATTTCAA ATCACTCGCT 
CAGTACCTGC ACTCGGGCCC ACCCGTGTAC TTTGTCCTGG AGGAAGGCTA TAACTACAGT 
TCACGCAAAG GGCAGAACAT GGTGTGCGGC GGCATGGGCT GTGACAATGA CTCCCTGGTG 
CAGCAGATAT TTAACGCAGC TGAGCTGGAC ACCTACACCC GAGTAGGCTT CGCCCCCTCG 
TCCTGGATCG ATGACTACTT TGACTGGGTC TCGCCACAGT CGTCCTGCTG CAGACTCTAC 
AACGTCACTC ACCAGTTCTG CAATGCTTCT GTGATGGACC CAACCTGTGT CCGCTGCAGA 
CCTCTGACTC CAGAGGGTAA ACAGAGGCCT CAGGGGAAAG AATTCATGAA ATTCCTGCCC 
ATGTTCCTTT CTGATAACCC CAACCCCAAG TGCGGCAAAG GGGGACATGC TGCTTACGGT 
TCAGCTGTTA ACATTGTGGG AGATGACACT TACATTGGGG CCACTTACTT CATGACCTAC 
CACACCATAC TTAAGACCTC CGCTGACTAT ACTGATGCCA TGAAGAAAGC TCGGCTAATA 
GCCAGTAACA TCACGGAAAC CATGCGTTCT AAGGGGAGTG ACTACCGCGT ATTCCCTTAC 
AGTGTGTTCT ACGTCTTCTA TGAACAGTAC CTGACCATTA TTGATGACAC CATCTTTAAC 
CTCAGTGTGT CTCTGGGCTC CATATTTCTG GTGACCTTGG TGGTTCTGGG CTGTGAGCTG 
TGGTCTGCGG TCATCATGTG TATCACCATA GCCATGATCC TGGTCAACAT GTTCGGTGTC 
ATGTGGCTGT GGGGCATCAG TCTGAATGCG GTCTCCTTGG TCAACTTGGT GATGAGCTGC 
GGCATTTCTG TGGAGTTCTG CAGCCATATA ACGAGAGCAT TCACCATGAG TACCAAAGGA 
AGCCGAGTGA GCCGGGCGGA AGAGGCACTG GCCCACATGG GTAGTTCTGT ATTCAGTGGA 
ATCACACTTA CGAAATTTGG AGGGATCGTG GTGTTAGCCT TTGCCAAATC TCAAATTTTT 
GAGATATTTT ACTTCAGGAT GTACTTAGCC ATGGTCTTAC TCGGAGCCAC TCATGGACTA 
ATATTTCTTC CCGTCTTACT CAGTTACATA GGGCCGTCGG TGAATAAAGC TAAAAGACAC 
ACCACTTACG AGCGCTACAG AGGGACAGAG AGAGAACGGC TCCTCAATTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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Npc1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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