Npc1 cDNA ORF clone, Rattus norvegicus(Norway rat)
| Gene Symbol | Npc1 |
| Entrez Gene ID | 266732 |
| Full Name | NPC intracellular cholesterol transporter 1 |
| Synonyms | Cdig2,SLC66A1 |
| General protein information |
|
| Gene Type | protein-coding |
| Organism | Rattus norvegicus(Norway rat) |
| Genome | |
| Summary | cadmium-inducible gene; transcription upregulated by cadmium [RGD, Feb 2006]. |
mRNA and Protein(s)
| mRNA | Protein | Name |
|---|---|---|
| NM_153624.2 | NP_705888.2 | Niemann-Pick C1 protein precursor |
| Canis lupus familiaris (dog) | NPC1 | NP_001003107.1 |
| Bos taurus (cattle) | NPC1 | NP_777183.1 |
| Kluyveromyces lactis | KLLA0F27137g | XP_456287.1 |
| Magnaporthe oryzae (rice blast fungus) | MGG_00819 | XP_003718164.1 |
| Mus musculus (house mouse) | Npc1 | NP_032746.2 |
| Drosophila melanogaster (fruit fly) | Npc1a | NP_001188769.1 |
| Homo sapiens (human) | NPC1 | NP_000262.2 |
| Pan troglodytes (chimpanzee) | NPC1 | XP_001155285.1 |
| NPC1 | XP_002800934.1 | |
| Anopheles gambiae (African malaria mosquito) | AgaP_AGAP008137 | XP_317322.4 |
| Rattus norvegicus (Norway rat) | Npc1 | NP_705888.2 |
| Danio rerio (zebrafish) | npc1 | NP_001230804.1 |
| Saccharomyces cerevisiae (baker's yeast) | NCR1 | NP_015319.1 |
| Eremothecium gossypii | AGOS_AGL008W | NP_986658.1 |
| Neurospora crassa | NCU04090 | XP_956086.1 |
| Arabidopsis thaliana (thale cress) | AT1G42470 | NP_174975.2 |
| Gallus gallus (chicken) | NPC1 | XP_419162.3 |
| Arabidopsis thaliana (thale cress) | AT4G38350 | NP_001190953.1 |
| Xenopus tropicalis (tropical clawed frog) | npc1 | XP_004915269.1 |
Related articles in PubMed
Reversal of Pathologic Lipid Accumulation in NPC1-Deficient Neurons by Drug-Promoted Release of LAMP1-Coated Lamellar Inclusions.
Demais V, Barth?l?my A, Perraut M, Ungerer N, Keime C, Reibel S, Pfrieger FW
The Journal of neuroscience : the official journal of the Society for Neuroscience36(30)8012-25(2016 07)
Interaction of AnxA6 with isolated and artificial lipid microdomains; importance of lipid composition and calcium content.
Domon MM, Besson F, Tylki-Szymanska A, Bandorowicz-Pikula J, Pikula S
Molecular bioSystems9(4)668-76(2013 Apr)
Characterization of the Niemann-Pick C pathway in alveolar type II cells and lamellar bodies of the lung.
Roszell BR, Tao JQ, Yu KJ, Huang S, Bates SR
American journal of physiology. Lung cellular and molecular physiology302(9)L919-32(2012 May)
A novel role for ?-tocopherol transfer protein (?-TTP) in protecting against chloroquine toxicity.
Shichiri M, Kono N, Shimanaka Y, Tanito M, Rotzoll DE, Yoshida Y, Hagihara Y, Tamai H, Arai H
The Journal of biological chemistry287(4)2926-34(2012 Jan)
Niemann-Pick type C 1 function requires lumenal domain residues that mediate cholesterol-dependent NPC2 binding.
Deffieu MS, Pfeffer SR
Proceedings of the National Academy of Sciences of the United States of America108(47)18932-6(2011 Nov)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
Here, we identify lamellar inclusions as the subcellular site of lipid accumulation in neurons, we uncover a vicious cycle of cholesterol synthesis and accretion, which may cause gradual neurodegeneration, and we reveal how beta-cyclodextrin, a potential therapeutic drug, reverts these changes. Our study provides new mechanistic insight in NPC disease and uncovers new targets for therapeutic approaches.
Title: Reversal of Pathologic Lipid Accumulation in NPC1-Deficient Neurons by Drug-Promoted Release of LAMP1-Coated Lamellar Inclusions.
NPC1 and NPC2 are associated with the lamellar bodies of type II pneumocyte cells and function to regulate cholesterol content within this organelle.
Title: Characterization of the Niemann-Pick C pathway in alveolar type II cells and lamellar bodies of the lung.
The following Npc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Npc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa03094 | |
| Clone ID Related Accession (Same CDS sequence) | NM_153624.2 | |
| Accession Version | NM_153624.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3837bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ® Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-10-02 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | Niemann-Pick C1 protein precursor | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07031186.1 and AABR07031184.1. On or before Nov 11, 2010 this sequence version replaced XM_002728521.1, XM_002725312.1, NM_153624.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGGCGCGC ACCACCCGGC CCTCGGCCTC CTGCTGCTGC TGCTGCTGTG CCCTGCGCAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_705888.2 |
| CDS | 96..3932 |
| Misc Feature(1) | 165..899 |
| Misc Feature(2) | 171..3851 |
| Misc Feature(3) | 2043..2504 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_153624.2 |
ORF Insert Sequence:
1 | ATGGGCGCGC ACCACCCGGC CCTCGGCCTC CTGCTGCTGC TGCTGCTGTG CCCTGCGCAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
 |
Reversal of Pathologic Lipid Accumulation in NPC1-Deficient Neurons by Drug-Promoted Release of LAMP1-Coated Lamellar Inclusions. |
|
Interaction of AnxA6 with isolated and artificial lipid microdomains; importance of lipid composition and calcium content. |
|
Characterization of the Niemann-Pick C pathway in alveolar type II cells and lamellar bodies of the lung. |
|
A novel role for ?-tocopherol transfer protein (?-TTP) in protecting against chloroquine toxicity. |
|
Niemann-Pick type C 1 function requires lumenal domain residues that mediate cholesterol-dependent NPC2 binding. |