Npc1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Npc1
Entrez Gene ID	266732
Full Name	NPC intracellular cholesterol transporter 1
Synonyms	Cdig2,SLC66A1
General protein information	Preferred Names NPC intracellular cholesterol transporter 1 Names Niemann-Pick C1 protein Niemann-Pick disease, type C1 solute carrier family 66 member 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 18 Map Location: 18p13
Summary	cadmium-inducible gene; transcription upregulated by cadmium [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_153624.2	NP_705888.2	Niemann-Pick C1 protein precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NPC1	NP_001003107.1
Bos taurus (cattle)	NPC1	NP_777183.1
Kluyveromyces lactis	KLLA0F27137g	XP_456287.1
Magnaporthe oryzae (rice blast fungus)	MGG_00819	XP_003718164.1
Mus musculus (house mouse)	Npc1	NP_032746.2
Drosophila melanogaster (fruit fly)	Npc1a	NP_001188769.1
Homo sapiens (human)	NPC1	NP_000262.2
Pan troglodytes (chimpanzee)	NPC1	XP_001155285.1
	NPC1	XP_002800934.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008137	XP_317322.4
Rattus norvegicus (Norway rat)	Npc1	NP_705888.2
Danio rerio (zebrafish)	npc1	NP_001230804.1
Saccharomyces cerevisiae (baker's yeast)	NCR1	NP_015319.1
Eremothecium gossypii	AGOS_AGL008W	NP_986658.1
Neurospora crassa	NCU04090	XP_956086.1
Arabidopsis thaliana (thale cress)	AT1G42470	NP_174975.2
Gallus gallus (chicken)	NPC1	XP_419162.3
Arabidopsis thaliana (thale cress)	AT4G38350	NP_001190953.1
Xenopus tropicalis (tropical clawed frog)	npc1	XP_004915269.1

Gene Ontology
Bibliography

Related articles in PubMed

3.3 ? structure of Niemann-Pick C1 protein reveals insights into the function of the C-terminal luminal domain in cholesterol transport.
Li X, Lu F, Trinh MN, Schmiege P, Seemann J, Wang J, Blobel G
Proceedings of the National Academy of Sciences of the United States of America114(34)9116-9121(2017 Aug)

Reversal of Pathologic Lipid Accumulation in NPC1-Deficient Neurons by Drug-Promoted Release of LAMP1-Coated Lamellar Inclusions.
Demais V, Barth?l?my A, Perraut M, Ungerer N, Keime C, Reibel S, Pfrieger FW
The Journal of neuroscience : the official journal of the Society for Neuroscience36(30)8012-25(2016 07)

Structural Insights into the Niemann-Pick C1 (NPC1)-Mediated Cholesterol Transfer and Ebola Infection.
Gong X, Qian H, Zhou X, Wu J, Wan T, Cao P, Huang W, Zhao X, Wang X, Wang P, Shi Y, Gao GF, Zhou Q, Yan N
Cell165(6)1467-1478(2016 Jun)

Interaction of AnxA6 with isolated and artificial lipid microdomains; importance of lipid composition and calcium content.
Domon MM, Besson F, Tylki-Szymanska A, Bandorowicz-Pikula J, Pikula S
Molecular bioSystems9(4)668-76(2013 Apr)

Characterization of the Niemann-Pick C pathway in alveolar type II cells and lamellar bodies of the lung.
Roszell BR, Tao JQ, Yu KJ, Huang S, Bates SR
American journal of physiology. Lung cellular and molecular physiology302(9)L919-32(2012 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Here, we identify lamellar inclusions as the subcellular site of lipid accumulation in neurons, we uncover a vicious cycle of cholesterol synthesis and accretion, which may cause gradual neurodegeneration, and we reveal how beta-cyclodextrin, a potential therapeutic drug, reverts these changes. Our study provides new mechanistic insight in NPC disease and uncovers new targets for therapeutic approaches.
Title: Reversal of Pathologic Lipid Accumulation in NPC1-Deficient Neurons by Drug-Promoted Release of LAMP1-Coated Lamellar Inclusions.

NPC1 and NPC2 are associated with the lamellar bodies of type II pneumocyte cells and function to regulate cholesterol content within this organelle.
Title: Characterization of the Niemann-Pick C pathway in alveolar type II cells and lamellar bodies of the lung.

The following Npc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Npc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa03094	NM_153624.2 Latest version!	Rattus norvegicus NPC intracellular cholesterol transporter 1 (Npc1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Npc1 ( NM_153624.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_705888.2 Rattus norvegicus NPC intracellular cholesterol transporter 1 (Npc1), mRNA.

Price & Availability↑

CloneID	ORa03094
Clone ID Related Accession (Same CDS sequence)	NM_153624.2
Accession Version	NM_153624.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	Niemann-Pick C1 protein precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07031186.1 and AABR07031184.1. On or before Nov 11, 2010 this sequence version replaced XM_002728521.1, XM_002725312.1, NM_153624.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCGCGC ACCACCCGGC CCTCGGCCTC CTGCTGCTGC TGCTGCTGTG CCCTGCGCAG 
GTGTTCTCAC AGTCCTGTGT CTGGTACGGA GAGTGTGGAG TCGCCTTTGG AGATAAGAGG 
TACAACTGTG AATATTCGGG CCCACCGAAA CCCCTCCCAA AGGACGGCTA TGACTTAGTG 
CAGGAACTCT GTCCAGGACT CTTCTTTGGC AATGTCAGTC TTTGCTGTGA CGTTCAGCAG 
CTTCAGACAC TGAAGAGCAA CCTGCAGCTG CCCCTGCAGT TTCTGTCCAG GTGTCCGTCA 
TGTTTTTATA ACCTGATGAC CCTGTTTTGT GAGCTAACAT GTAGCCCGCA CCAGAGTCAG 
TTTCTGAATG TGACAGCAAC TGAAGATTAT TTCGACCCCG AGACTCGGGA AAATAAAACA 
AATGTAAAGG AATTAGAGTA CTACGTCGGA CGGAGCTTTG CGAATGCAAT GTACAATGCC 
TGCCGAGATG TGGAGGCGCC TTCCAGTAAT GAGAAGGCCT TAGGACTCCT GTGCGGAAGG 
GATGCCCGTG CCTGCAATGC CACCAACTGG ATTGAGTACA TGTTCAACAA AGACAACGGG 
CAAGCACCGT TTACCATCAT CCCCGTATTT TCAGATCTTT CGGTTCTTGG AATGGAACCC 
ATGAGGAATG CCACCAAAGG CTGCAACGAG TCTGTAGATG AGGTCACAGG GCCGTGTAGC 
TGCCAGGACT GCTCTGCCGT CTGCGGCCCC AAACCCCAGC CCCCACCGCC CCCTGTGCCC 
TGGAGGATCT TAGGTTTGGA CGCCATGTAT GTCATCATGT GGGTCGCCTA CATGGCGTTC 
CTGGTCCTGT TCTTTGGAGG ACTGCTGGCA GTGTGGTGCC ACAGAAGGCG GTACTTTGTG 
TCCGAGTACA CCCCCATTGA CAGCAACATT GCCTTTTCCT CGAATAGCAG TGACAAAGGG 
GAAGCCTCGT GCTGCGACCC ACTTGGTGTA GCGTTTGATG ACTGTCTGAG GCGCATGTTC 
ACAAAGTGGG GGGCTTTCTG TGTCCGGAAT CCCACCTGCA TCATTTTCTT CTCGTTGGTC 
TTCATTGCTG CGTGCTCTTC TGGCCTGGTG TTTGTCCAGG TCACCACCAA TCCCGTGGAG 
CTCTGGTCAG CCCCTCACAG TCAGGCCCGC TTGGAAAAGG AGTACTTTGA CAAGCACTTT 
GGGCCTTTCT TTCGTACGGA GCAGCTTATT ATCCAGGCCC CCAACTCCAG CGTCCATATC 
TACGAGCCGT ACCCTTCCGG AGCCGATGTG CCCTTCGGGC CTCCATTGAA CAAAGAGATT 
CTGCACCAGG TTCTGGACTT ACAGATCGCC ATTGAAAGTA TCACTGCGTC CTATAACAAT 
GAAACCGTGA CACTTCAGGA CATCTGTGTG GCCCCCCTCT CTCCATACAA CAAGAACTGC 
ACCATTATGA GTGTGTTAAA TTACTTCCAG AACAGCCATG CGGTGCTGGA CAACCAAGTA 
GGCGATGACT TCTTTGTCTA TGCCGATTAC CACACGCACT TTCTGTACTG TGTGAGGGCT 
CCCGCCTCCT TGAATGATAC GAGTTTGCTC CACGGCCCTT GCCTGGGCAC ATTTGGAGGA 
CCTGTGTTCC CGTGGCTTGT GTTGGGCGGC TATGATGATC AGAACTACAA TAATGCCACC 
GCGCTTGTGA TTACCTTCCC TGTCAATAAT TACTATAATG ACACAGAGAA GCTCCAGAGG 
GCCTGGGCCT GGGAGAAAGA ATTTATTAAC TTTGTGAAGA ACTACAAGAA TCCAAATCTG 
ACCATTTCTT TCACTGCTGA GCGAAGCATT GAAGATGAAC TCAATCGGGA AAGCAACAGT 
GACGTATTCA CCGTCCTCAT CAGCTATGCC GTGATGTTTC TGTACATTTC CCTCGCCCTG 
GGGCACATCC AGAGCTGGAA CAGGGTCCTG GTGGATTCTA AAATCTCGCT GGGCATTGCG 
GGGATCCTGA TAGTGCTGAG CTCGGTGACC TGCTCCCTGG GCATCTTCAG CTACATCGGA 
ATGCCACTGA CCCTCATCGT CATTGAGGTC ATCCCGTTCC TGGTGCTGGC TGTTGGGGTG 
GACAACATCT TCATTCTAGT GCAGACCTAC CAGAGAGATG AGCGTCTTCA GGAGGAGACG 
TTGGATCAGC AGCTGGGCAG GATCCTGGGG GAAGTGGCCC CAACCATGTT CCTTTCGTCC 
TTCTCTGAGA CCTCGGCATT TTTCTTTGGG GCGCTGTCCT CGATGCCAGC TGTCCACACC 
TTCTCTCTGT TTGCGGGGAT GGCCGTCCTC ATTGACTTCC TCCTTCAGAT TACCTGCTTT 
GTGAGCTTCT TGGGGTTAGA TATTAAGAGA CAAGAGAAAA ACCGTCTGGA CATCCTGTGC 
TGTGTCAGAG GCCCTGACGA CGGACAAGAA TCCCAAGCCT CCGAAAGCTA CCTGTTCCGC 
TTCTTCAAAA ACGCCTTTGC CCCATTTCTG CTGACGGACT GGCTGCGGCC AGTTGTGATG 
GCCGTGTTTG TGGGCATTCT GTCATTCAGT ATTGCAGTAG TGAACAAAGT AGAAATCGGA 
TTGGATCAGT CTCTCTCGAT GCCAAATGAT TCGTATGTGA TTGACTATTT CAAGTCACTC 
GGTCAGTACC TGCACTCGGG CCCACCCGTG TACTTCGTCC TGGAAGAGGG CTACAACTAC 
AGTTCCCGCA GAGGGCAGAA CATGGTGTGC GGTGGCATGG GCTGTGACAA TGACTCCCTG 
GTGCAGCAGA TATTTAACGC AGCAGAGCTG GACACCTACA CCCGCGTAGG CTTCGCTCCC 
TCATCCTGGA TTGATGATTA CTTTGACTGG GTCTCACCGC AATCCTCCTG CTGTAGACTC 
TACAATGTCA CTCACCAGTT CTGCAATGCT TCTGTGATTG ACCCAACCTG TGTCCGCTGC 
AGACCTCTGA CTCCAGAGGG CAAACAGAGG CCTCAGGGGA AAGAATTCAT GAAATTCCTG 
CCCATGTTCC TGTCTGATAA CCCCAACCCC AAGTGTGGCA AAGGGGGACA TGCTGCTTAC 
AGTTCTGCTG TTAACATCAT GGGAGATGAC ACTTACATTG GGGCCACTTA CTTCATGACC 
TACCACACCA TTCTTAAGAC CTCTGCTGAT TATATCGATG CCCTGAAGAA AGCTCGGCTT 
ATAGCCAGTA ACATCACGGA AACCATGCGT TCTAAGGGGA GTGATTATCG TGTGTTTCCT 
TACAGTGTGT TCTATGTCTT CTATGAACAA TACCTGACCA TTATTGATGA CGCCATCTTT 
AACCTAAGTG TGTCTCTGGG CTCCATATTT CTCGTGACCT TGGTGGCTCT GGGCTGTGAA 
CTGTGGTCTG CGGTCATCAT GTGTCTCACC ATAGCTATGA TCCTGGTCAA CATGTTTGGT 
GTTATGTGGC TGTGGGGCAT CAGTCTGAAC GCTGTTTCCT TGGTCAACTT GGTGATGACC 
TGTGGCATTT CTGTGGAGTT CTGCAGCCAT ATAACAAGAG CTTTCACCAT GAGCACCAAA 
GGAAGCAGAG TGAGCAGGGC GGAAGAGGCA CTGGCCCACA TGGGTAGTTC TGTATTCAGT 
GGAATCACAC TTACAAAATT TGGAGGGATC GTGGTGTTGG CCTTTGCCAA GTCTCAAATT 
TTTGAGATCT TTTACTTCAG GATGTACTTA GCCATGGTCT TACTTGGAGC TACTCACGGA 
CTAATATTTC TTCCCGTCTT ACTCAGTTAC ATAGGGCCAT CAGTGAATAA AGCTAAAAGA 
CACACCACTC AAGAGCGGTA CAAAGGGACA GAGAGAGAAC GGCTCCTCAA TTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_705888.2
CDS	96..3932
Translation	MGAHHPALGLLLLLLLCPAQVFSQSCVWYGECGVAFGDKRYNCEYSGPPKPLPKDGYDLVQELCPGLFFGNVSLCCDVQQLQTLKSNLQLPLQFLSRCPSCFYNLMTLFCELTCSPHQSQFLNVTATEDYFDPETRENKTNVKELEYYVGRSFANAMYNACRDVEAPSSNEKALGLLCGRDARACNATNWIEYMFNKDNGQAPFTIIPVFSDLSVLGMEPMRNATKGCNESVDEVTGPCSCQDCSAVCGPKPQPPPPPVPWRILGLDAMYVIMWVAYMAFLVLFFGGLLAVWCHRRRYFVSEYTPIDSNIAFSSNSSDKGEASCCDPLGVAFDDCLRRMFTKWGAFCVRNPTCIIFFSLVFIAACSSGLVFVQVTTNPVELWSAPHSQARLEKEYFDKHFGPFFRTEQLIIQAPNSSVHIYEPYPSGADVPFGPPLNKEILHQVLDLQIAIESITASYNNETVTLQDICVAPLSPYNKNCTIMSVLNYFQNSHAVLDNQVGDDFFVYADYHTHFLYCVRAPASLNDTSLLHGPCLGTFGGPVFPWLVLGGYDDQNYNNATALVITFPVNNYYNDTEKLQRAWAWEKEFINFVKNYKNPNLTISFTAERSIEDELNRESNSDVFTVLISYAVMFLYISLALGHIQSWNRVLVDSKISLGIAGILIVLSSVTCSLGIFSYIGMPLTLIVIEVIPFLVLAVGVDNIFILVQTYQRDERLQEETLDQQLGRILGEVAPTMFLSSFSETSAFFFGALSSMPAVHTFSLFAGMAVLIDFLLQITCFVSFLGLDIKRQEKNRLDILCCVRGPDDGQESQASESYLFRFFKNAFAPFLLTDWLRPVVMAVFVGILSFSIAVVNKVEIGLDQSLSMPNDSYVIDYFKSLGQYLHSGPPVYFVLEEGYNYSSRRGQNMVCGGMGCDNDSLVQQIFNAAELDTYTRVGFAPSSWIDDYFDWVSPQSSCCRLYNVTHQFCNASVIDPTCVRCRPLTPEGKQRPQGKEFMKFLPMFLSDNPNPKCGKGGHAAYSSAVNIMGDDTYIGATYFMTYHTILKTSADYIDALKKARLIASNITETMRSKGSDYRVFPYSVFYVFYEQYLTIIDDAIFNLSVSLGSIFLVTLVALGCELWSAVIMCLTIAMILVNMFGVMWLWGISLNAVSLVNLVMTCGISVEFCSHITRAFTMSTKGSRVSRAEEALAHMGSSVFSGITLTKFGGIVVLAFAKSQIFEIFYFRMYLAMVLLGATHGLIFLPVLLSYIGPSVNKAKRHTTQERYKGTERERLLNF

Target ORF information:

RefSeq Version	NM_153624.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NPC intracellular cholesterol transporter 1 (Npc1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153624.2

ORF Insert Sequence:

ATGGGCGCGC ACCACCCGGC CCTCGGCCTC CTGCTGCTGC TGCTGCTGTG CCCTGCGCAG 
GTGTTCTCAC AGTCCTGTGT CTGGTACGGA GAGTGTGGAG TCGCCTTTGG AGATAAGAGG 
TACAACTGTG AATATTCGGG CCCACCGAAA CCCCTCCCAA AGGACGGCTA TGACTTAGTG 
CAGGAACTCT GTCCAGGACT CTTCTTTGGC AATGTCAGTC TTTGCTGTGA CGTTCAGCAG 
CTTCAGACAC TGAAGAGCAA CCTGCAGCTG CCCCTGCAGT TTCTGTCCAG GTGTCCGTCA 
TGTTTTTATA ACCTGATGAC CCTGTTTTGT GAGCTAACAT GTAGCCCGCA CCAGAGTCAG 
TTTCTGAATG TGACAGCAAC TGAAGATTAT TTCGACCCCG AGACTCGGGA AAATAAAACA 
AATGTAAAGG AATTAGAGTA CTACGTCGGA CGGAGCTTTG CGAATGCAAT GTACAATGCC 
TGCCGAGATG TGGAGGCGCC TTCCAGTAAT GAGAAGGCCT TAGGACTCCT GTGCGGAAGG 
GATGCCCGTG CCTGCAATGC CACCAACTGG ATTGAGTACA TGTTCAACAA AGACAACGGG 
CAAGCACCGT TTACCATCAT CCCCGTATTT TCAGATCTTT CGGTTCTTGG AATGGAACCC 
ATGAGGAATG CCACCAAAGG CTGCAACGAG TCTGTAGATG AGGTCACAGG GCCGTGTAGC 
TGCCAGGACT GCTCTGCCGT CTGCGGCCCC AAACCCCAGC CCCCACCGCC CCCTGTGCCC 
TGGAGGATCT TAGGTTTGGA CGCCATGTAT GTCATCATGT GGGTCGCCTA CATGGCGTTC 
CTGGTCCTGT TCTTTGGAGG ACTGCTGGCA GTGTGGTGCC ACAGAAGGCG GTACTTTGTG 
TCCGAGTACA CCCCCATTGA CAGCAACATT GCCTTTTCCT CGAATAGCAG TGACAAAGGG 
GAAGCCTCGT GCTGCGACCC ACTTGGTGTA GCGTTTGATG ACTGTCTGAG GCGCATGTTC 
ACAAAGTGGG GGGCTTTCTG TGTCCGGAAT CCCACCTGCA TCATTTTCTT CTCGTTGGTC 
TTCATTGCTG CGTGCTCTTC TGGCCTGGTG TTTGTCCAGG TCACCACCAA TCCCGTGGAG 
CTCTGGTCAG CCCCTCACAG TCAGGCCCGC TTGGAAAAGG AGTACTTTGA CAAGCACTTT 
GGGCCTTTCT TTCGTACGGA GCAGCTTATT ATCCAGGCCC CCAACTCCAG CGTCCATATC 
TACGAGCCGT ACCCTTCCGG AGCCGATGTG CCCTTCGGGC CTCCATTGAA CAAAGAGATT 
CTGCACCAGG TTCTGGACTT ACAGATCGCC ATTGAAAGTA TCACTGCGTC CTATAACAAT 
GAAACCGTGA CACTTCAGGA CATCTGTGTG GCCCCCCTCT CTCCATACAA CAAGAACTGC 
ACCATTATGA GTGTGTTAAA TTACTTCCAG AACAGCCATG CGGTGCTGGA CAACCAAGTA 
GGCGATGACT TCTTTGTCTA TGCCGATTAC CACACGCACT TTCTGTACTG TGTGAGGGCT 
CCCGCCTCCT TGAATGATAC GAGTTTGCTC CACGGCCCTT GCCTGGGCAC ATTTGGAGGA 
CCTGTGTTCC CGTGGCTTGT GTTGGGCGGC TATGATGATC AGAACTACAA TAATGCCACC 
GCGCTTGTGA TTACCTTCCC TGTCAATAAT TACTATAATG ACACAGAGAA GCTCCAGAGG 
GCCTGGGCCT GGGAGAAAGA ATTTATTAAC TTTGTGAAGA ACTACAAGAA TCCAAATCTG 
ACCATTTCTT TCACTGCTGA GCGAAGCATT GAAGATGAAC TCAATCGGGA AAGCAACAGT 
GACGTATTCA CCGTCCTCAT CAGCTATGCC GTGATGTTTC TGTACATTTC CCTCGCCCTG 
GGGCACATCC AGAGCTGGAA CAGGGTCCTG GTGGATTCTA AAATCTCGCT GGGCATTGCG 
GGGATCCTGA TAGTGCTGAG CTCGGTGACC TGCTCCCTGG GCATCTTCAG CTACATCGGA 
ATGCCACTGA CCCTCATCGT CATTGAGGTC ATCCCGTTCC TGGTGCTGGC TGTTGGGGTG 
GACAACATCT TCATTCTAGT GCAGACCTAC CAGAGAGATG AGCGTCTTCA GGAGGAGACG 
TTGGATCAGC AGCTGGGCAG GATCCTGGGG GAAGTGGCCC CAACCATGTT CCTTTCGTCC 
TTCTCTGAGA CCTCGGCATT TTTCTTTGGG GCGCTGTCCT CGATGCCAGC TGTCCACACC 
TTCTCTCTGT TTGCGGGGAT GGCCGTCCTC ATTGACTTCC TCCTTCAGAT TACCTGCTTT 
GTGAGCTTCT TGGGGTTAGA TATTAAGAGA CAAGAGAAAA ACCGTCTGGA CATCCTGTGC 
TGTGTCAGAG GCCCTGACGA CGGACAAGAA TCCCAAGCCT CCGAAAGCTA CCTGTTCCGC 
TTCTTCAAAA ACGCCTTTGC CCCATTTCTG CTGACGGACT GGCTGCGGCC AGTTGTGATG 
GCCGTGTTTG TGGGCATTCT GTCATTCAGT ATTGCAGTAG TGAACAAAGT AGAAATCGGA 
TTGGATCAGT CTCTCTCGAT GCCAAATGAT TCGTATGTGA TTGACTATTT CAAGTCACTC 
GGTCAGTACC TGCACTCGGG CCCACCCGTG TACTTCGTCC TGGAAGAGGG CTACAACTAC 
AGTTCCCGCA GAGGGCAGAA CATGGTGTGC GGTGGCATGG GCTGTGACAA TGACTCCCTG 
GTGCAGCAGA TATTTAACGC AGCAGAGCTG GACACCTACA CCCGCGTAGG CTTCGCTCCC 
TCATCCTGGA TTGATGATTA CTTTGACTGG GTCTCACCGC AATCCTCCTG CTGTAGACTC 
TACAATGTCA CTCACCAGTT CTGCAATGCT TCTGTGATTG ACCCAACCTG TGTCCGCTGC 
AGACCTCTGA CTCCAGAGGG CAAACAGAGG CCTCAGGGGA AAGAATTCAT GAAATTCCTG 
CCCATGTTCC TGTCTGATAA CCCCAACCCC AAGTGTGGCA AAGGGGGACA TGCTGCTTAC 
AGTTCTGCTG TTAACATCAT GGGAGATGAC ACTTACATTG GGGCCACTTA CTTCATGACC 
TACCACACCA TTCTTAAGAC CTCTGCTGAT TATATCGATG CCCTGAAGAA AGCTCGGCTT 
ATAGCCAGTA ACATCACGGA AACCATGCGT TCTAAGGGGA GTGATTATCG TGTGTTTCCT 
TACAGTGTGT TCTATGTCTT CTATGAACAA TACCTGACCA TTATTGATGA CGCCATCTTT 
AACCTAAGTG TGTCTCTGGG CTCCATATTT CTCGTGACCT TGGTGGCTCT GGGCTGTGAA 
CTGTGGTCTG CGGTCATCAT GTGTCTCACC ATAGCTATGA TCCTGGTCAA CATGTTTGGT 
GTTATGTGGC TGTGGGGCAT CAGTCTGAAC GCTGTTTCCT TGGTCAACTT GGTGATGACC 
TGTGGCATTT CTGTGGAGTT CTGCAGCCAT ATAACAAGAG CTTTCACCAT GAGCACCAAA 
GGAAGCAGAG TGAGCAGGGC GGAAGAGGCA CTGGCCCACA TGGGTAGTTC TGTATTCAGT 
GGAATCACAC TTACAAAATT TGGAGGGATC GTGGTGTTGG CCTTTGCCAA GTCTCAAATT 
TTTGAGATCT TTTACTTCAG GATGTACTTA GCCATGGTCT TACTTGGAGC TACTCACGGA 
CTAATATTTC TTCCCGTCTT ACTCAGTTAC ATAGGGCCAT CAGTGAATAA AGCTAAAAGA 
CACACCACTC AAGAGCGGTA CAAAGGGACA GAGAGAGAAC GGCTCCTCAA TTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

3.3 ? structure of Niemann-Pick C1 protein reveals insights into the function of the C-terminal luminal domain in cholesterol transport.
Proceedings of the National Academy of Sciences of the United States of America114(34)9116-9121(2017 Aug)
Li X,Lu F,Trinh MN,Schmiege P,Seemann J,Wang J,Blobel G

Reversal of Pathologic Lipid Accumulation in NPC1-Deficient Neurons by Drug-Promoted Release of LAMP1-Coated Lamellar Inclusions.
The Journal of neuroscience : the official journal of the Society for Neuroscience36(30)8012-25(2016 07)
Demais V,Barth?l?my A,Perraut M,Ungerer N,Keime C,Reibel S,Pfrieger FW

Structural Insights into the Niemann-Pick C1 (NPC1)-Mediated Cholesterol Transfer and Ebola Infection.
Cell165(6)1467-1478(2016 Jun)
Gong X,Qian H,Zhou X,Wu J,Wan T,Cao P,Huang W,Zhao X,Wang X,Wang P,Shi Y,Gao GF,Zhou Q,Yan N

Interaction of AnxA6 with isolated and artificial lipid microdomains; importance of lipid composition and calcium content.
Molecular bioSystems9(4)668-76(2013 Apr)
Domon MM,Besson F,Tylki-Szymanska A,Bandorowicz-Pikula J,Pikula S

Characterization of the Niemann-Pick C pathway in alveolar type II cells and lamellar bodies of the lung.
American journal of physiology. Lung cellular and molecular physiology302(9)L919-32(2012 May)
Roszell BR,Tao JQ,Yu KJ,Huang S,Bates SR

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Npc1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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