Gene Symbol | Wnt5b |
Entrez Gene ID | 22419 |
Full Name | wingless-type MMTV integration site family, member 5B |
Synonyms | AW545702,Wnt-5b |
General protein information |
|
Gene Type | protein-coding |
Organism | Mus musculus(house mouse) |
Genome |
ORF » Species Summary » Mus musculus » Wnt5b cDNA ORF clone
gRNAs
Gene Symbol | Wnt5b |
Entrez Gene ID | 22419 |
Full Name | wingless-type MMTV integration site family, member 5B |
Synonyms | AW545702,Wnt-5b |
General protein information |
|
Gene Type | protein-coding |
Organism | Mus musculus(house mouse) |
Genome |
mRNA | Protein | Name |
---|---|---|
XM_017321520.1 | XP_017177009.1 | protein Wnt-5b isoform X1 |
XM_006505924.1 | XP_006505987.1 | protein Wnt-5b isoform X2 |
XM_030255319.1 | XP_030111179.1 | protein Wnt-5b isoform X1 |
NM_001271758.1 | NP_001258687.1 | protein Wnt-5b precursor |
NM_009525.3 | NP_033551.2 | protein Wnt-5b precursor |
NM_001271757.1 | NP_001258686.1 | protein Wnt-5b precursor |
Pan troglodytes (chimpanzee) | WNT5B | XP_522589.3 |
LOC721685 | XP_001117880.2 | |
Bos taurus (cattle) | WNT5B | NP_001192557.1 |
Danio rerio (zebrafish) | wnt5b | NP_571012.1 |
Anopheles gambiae (African malaria mosquito) | AgaP_AGAP010290 | XP_319487.4 |
Xenopus tropicalis (tropical clawed frog) | wnt5b | NP_001006767.1 |
Rattus norvegicus (Norway rat) | Wnt5b | NP_001093959.1 |
Gallus gallus (chicken) | WNT5B | NP_001032346.1 |
Canis lupus familiaris (dog) | WNT5B | XP_543883.3 |
Mus musculus (house mouse) | Wnt5b | NP_001258686.1 |
Caenorhabditis elegans (roundworm) | cwn-2 | NP_501822.1 |
Homo sapiens (human) | WNT5B | NP_110402.2 |
Arid1b haploinsufficiency disrupts cortical interneuron development and mouse behavior.
Jung EM, Moffat JJ, Liu J, Dravid SM, Gurumurthy CB, Kim WY
Nature neuroscience20(12)1694-1707(2017 Dec)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
opposing gradients of Wnt5a and Wnt5b and of their Sfrp inhibitors, together with intercellular signaling via planar cell polarity proteins, polarize node cells along the anterior-posterior axis for breaking of left-right symmetry.
Title: A Wnt5 Activity Asymmetry and Intercellular Signaling via PCP Proteins Polarize Node Cells for Left-Right Symmetry Breaking.
Wnt5b activated known calcium-dependent signaling pathways and JNK, a component of the planar cell polarity pathway.
Title: Wnt5b regulates mesenchymal cell aggregation and chondrocyte differentiation through the planar cell polarity pathway.
this study aimed at defining the expression profile and function of Wnt5a and Wnt5b during adipogenesis by determining their effect on aP2 and PPARgamma expression and assessing the level of beta-catenin translocation in mouse 3T3-L1 preadipocytes.
Title: Wnt5b stimulates adipogenesis by activating PPARgamma, and inhibiting the beta-catenin dependent Wnt signaling pathway together with Wnt5a.
hCG was found to up-regulate these wnt ligands in mouse mammary gland, independent of the changes in ovarian steroidogenesis
Title: Human chorionic gonadotropin (hCG) up-regulates wnt5b and wnt7b in the mammary gland, and hCGbeta transgenic female mice present with mammary Gland tumors exhibiting characteristics of the Wnt/beta-catenin pathway activation.
Wnt5b may promote adipogenesis in 3T3-L1 cells, at least in part, by antagonizing the canonical Wnt/beta-catenin pathway
Title: Wnt5b partially inhibits canonical Wnt/beta-catenin signaling pathway and promotes adipogenesis in 3T3-L1 preadipocytes.
The following Wnt5b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Wnt5b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
CloneID | OMu23647 | |
Clone ID Related Accession (Same CDS sequence) | NM_001271758.1 , NM_009525.3 , NM_001271757.1 | |
Accession Version | NM_001271758.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1080bp) Protein sequence SNP |
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Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-12-16 | |
Organism | Mus musculus(house mouse) | |
Product | protein Wnt-5b precursor | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC126607.7. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC010775.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849378 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. |
1 | ATGCCCAGCC TGCTGCTGGT GGTCGTGGCA GCTCTGCTCT CCAGCTGGGC ACAGCTGCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001258687.1 |
CDS | 171..1250 |
Translation |
Target ORF information:
Target ORF information:
|
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1 | ATGCCCAGCC TGCTGCTGGT GGTCGTGGCA GCTCTGCTCT CCAGCTGGGC ACAGCTGCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu23647 | |
Clone ID Related Accession (Same CDS sequence) | NM_001271758.1 , NM_009525.3 , NM_001271757.1 | |
Accession Version | NM_009525.3 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1080bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-12-16 | |
Organism | Mus musculus(house mouse) | |
Product | protein Wnt-5b precursor | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY739600.1, AK013218.1, BU520338.1, AC126607.7 and BG146815.1. On Oct 16, 2007 this sequence version replaced NM_009525.2. Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK013218.1, BC051406.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. |
1 | ATGCCCAGCC TGCTGCTGGT GGTCGTGGCA GCTCTGCTCT CCAGCTGGGC ACAGCTGCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_033551.2 |
CDS | 425..1504 |
Translation |
Target ORF information:
Target ORF information:
|
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1 | ATGCCCAGCC TGCTGCTGGT GGTCGTGGCA GCTCTGCTCT CCAGCTGGGC ACAGCTGCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu23647 | |
Clone ID Related Accession (Same CDS sequence) | NM_001271758.1 , NM_009525.3 , NM_001271757.1 | |
Accession Version | NM_001271757.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1080bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-12-16 | |
Organism | Mus musculus(house mouse) | |
Product | protein Wnt-5b precursor | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC126607.7 and AK049558.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK049558.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. |
1 | ATGCCCAGCC TGCTGCTGGT GGTCGTGGCA GCTCTGCTCT CCAGCTGGGC ACAGCTGCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001258686.1 |
CDS | 228..1307 |
Translation |
Target ORF information:
Target ORF information:
|
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1 | ATGCCCAGCC TGCTGCTGGT GGTCGTGGCA GCTCTGCTCT CCAGCTGGGC ACAGCTGCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu37140 | |
Clone ID Related Accession (Same CDS sequence) | XM_006505924.1 | |
Accession Version | XM_006505924.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1119bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-08-07 | |
Organism | Mus musculus(house mouse) | |
Product | protein Wnt-5b isoform X2 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGTTGGTCC CAGGGCATTG GGATGGGTTG AGGCCGGCCA TGCCCAGCCT GCTGCTGGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_006505987.1 |
CDS | 304..1422 |
Translation |
Target ORF information:
Target ORF information:
|
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1 | ATGTTGGTCC CAGGGCATTG GGATGGGTTG AGGCCGGCCA TGCCCAGCCT GCTGCTGGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu81691 | |
Clone ID Related Accession (Same CDS sequence) | XM_017321520.1 , XM_030255319.1 | |
Accession Version | XM_017321520.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2016-06-21 | |
Organism | Mus musculus(house mouse) | |
Product | protein Wnt-5b isoform X1 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGCGGCTA GGTGCCAAGA GGGAGCTCCG CTTTGGAAGA TGTTGGTCCC AGGGCATTGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_017177009.1 |
CDS | 106..1263 |
Translation |
Target ORF information:
Target ORF information:
|
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1 | ATGGCGGCTA GGTGCCAAGA GGGAGCTCCG CTTTGGAAGA TGTTGGTCCC AGGGCATTGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu81691 | |
Clone ID Related Accession (Same CDS sequence) | XM_017321520.1 , XM_030255319.1 | |
Accession Version | XM_030255319.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-08-07 | |
Organism | Mus musculus(house mouse) | |
Product | protein Wnt-5b isoform X1 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGCGGCTA GGTGCCAAGA GGGAGCTCCG CTTTGGAAGA TGTTGGTCCC AGGGCATTGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_030111179.1 |
CDS | 126..1283 |
Translation |
Target ORF information:
Target ORF information:
|
![]() |
1 | ATGGCGGCTA GGTGCCAAGA GGGAGCTCCG CTTTGGAAGA TGTTGGTCCC AGGGCATTGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Arid1b haploinsufficiency disrupts cortical interneuron development and mouse behavior. |