Tmem53 cDNA ORF clone, Mus musculus(house mouse)

  • Gene

  • Clones

  • gRNAs

The following Tmem53 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tmem53 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu17312 NM_001285812.1
Latest version!
Mus musculus transmembrane protein 53 (Tmem53), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
OMu17356 NM_001285814.1
Latest version!
Mus musculus transmembrane protein 53 (Tmem53), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
OMu17356 NM_029589.1
Latest version!
Mus musculus transmembrane protein 53 (Tmem53), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
OMu03137 NM_026837.3
Latest version!
Mus musculus transmembrane protein 53 (Tmem53), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $188.30
$269.00
OMu08888 NM_001285816.1
Latest version!
Mus musculus transmembrane protein 53 (Tmem53), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $139.30
$199.00
OMu08888 NM_001285815.1
Latest version!
Mus musculus transmembrane protein 53 (Tmem53), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $139.30
$199.00
OMu08888 XM_030253755.1
Latest version!
Mus musculus transmembrane protein 53 (Tmem53), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu17312
    Clone ID Related Accession (Same CDS sequence) NM_001285812.1
    Accession Version NM_001285812.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 852bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Mus musculus(house mouse)
    Product transmembrane protein 53 isoform a
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL671866.8, AK004164.1, AW049729.1 and AL844169.6. Transcript Variant: This variant (1) encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC020121.1, BF164608.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGCCTCGG CCGAGCTGGA TTACAGCATC GAGATCCCGG ATCAGCCCTG CTGGAGCCAG 
    AGTATGGAGG GGGATTGGGA AGAGAACAGA CAAGGTGGGA AGGAAGCTGG GAAGCAACAG
    CCTGTGGTGA TTCTCTTGGG CTGGGGAGGC TGCAGGGACA AGAACCTGGC CAAGTATAGT
    GCTATCTACC ACAAAAGGGG CTGCATTGTG ATCCGATACA CGGCCCCCTG GCACATGGTC
    TTCTTCTCTG AGTCCTTGGG CATCCCTTCA CTTCGCGTGA TAGCCCAGAA ACTGCTGGAG
    CTCCTCTTTG ACTATGAGAT TGAGCGGGAG CCTCTGCTCT TCCACGTCTT CAGCAATGCC
    GGCGTCATGC TCTACCGCTA CGTGCTGGAG CTCCTACAGA CCCACCAGCG CTTCCGCCAT
    TTGCATGTGG TGGGCACCAT CTTTGACAGT GGTCCTGGCG ATAGCAACCT GATAGGGGCC
    CTGAGGGCTC TGGCAACCAT CCTGGAGCGC CGACCTGCGG TGCTGCGCCT GTTGCTCCTG
    GCAGCATTTG CCCTGGTGGT GATCCTGTTT CACTTCCTGC TTGCTCCATT CACTGCCCTC
    TTCCACACCC ACTTCTACGA CAGGCTGCAG GACTCAGGCT CCTGCTGGCC TGAACTCTAC
    CTCTACTCTA GAGCTGATAA GGTGGTCTCG GCCAGGGATG TGGAACGTAT GGTAGAGGCA
    CGCCTGGCTC ACCAGGTCAT GGTGCGTGGA GTGGACTTTG TGTCATCTGC GCATGTCAGC
    CACCTCCGAG ACTATCCTAC TTACTATACA AGTCTGTGTG TTGACTTCAT GCATAATTGT
    GTCCAATGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001272741.1
    CDS124..975
    Translation

    Target ORF information:

    RefSeq Version NM_001285812.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus transmembrane protein 53 (Tmem53), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001285812.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGCCTCGG CCGAGCTGGA TTACAGCATC GAGATCCCGG ATCAGCCCTG CTGGAGCCAG 
    AGTATGGAGG GGGATTGGGA AGAGAACAGA CAAGGTGGGA AGGAAGCTGG GAAGCAACAG
    CCTGTGGTGA TTCTCTTGGG CTGGGGAGGC TGCAGGGACA AGAACCTGGC CAAGTATAGT
    GCTATCTACC ACAAAAGGGG CTGCATTGTG ATCCGATACA CGGCCCCCTG GCACATGGTC
    TTCTTCTCTG AGTCCTTGGG CATCCCTTCA CTTCGCGTGA TAGCCCAGAA ACTGCTGGAG
    CTCCTCTTTG ACTATGAGAT TGAGCGGGAG CCTCTGCTCT TCCACGTCTT CAGCAATGCC
    GGCGTCATGC TCTACCGCTA CGTGCTGGAG CTCCTACAGA CCCACCAGCG CTTCCGCCAT
    TTGCATGTGG TGGGCACCAT CTTTGACAGT GGTCCTGGCG ATAGCAACCT GATAGGGGCC
    CTGAGGGCTC TGGCAACCAT CCTGGAGCGC CGACCTGCGG TGCTGCGCCT GTTGCTCCTG
    GCAGCATTTG CCCTGGTGGT GATCCTGTTT CACTTCCTGC TTGCTCCATT CACTGCCCTC
    TTCCACACCC ACTTCTACGA CAGGCTGCAG GACTCAGGCT CCTGCTGGCC TGAACTCTAC
    CTCTACTCTA GAGCTGATAA GGTGGTCTCG GCCAGGGATG TGGAACGTAT GGTAGAGGCA
    CGCCTGGCTC ACCAGGTCAT GGTGCGTGGA GTGGACTTTG TGTCATCTGC GCATGTCAGC
    CACCTCCGAG ACTATCCTAC TTACTATACA AGTCTGTGTG TTGACTTCAT GCATAATTGT
    GTCCAATGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu17356
    Clone ID Related Accession (Same CDS sequence) NM_029589.1 , NM_001285814.1
    Accession Version NM_029589.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 786bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Mus musculus(house mouse)
    Product transmembrane protein 53 isoform c
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK005885.1, AK005104.1 and AL844169.6. Transcript Variant: This variant (3) includes alternate 5' exon structure and it thus differs in its 5' UTR and 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (c) has a distinct and shorter N-terminus, compared to isoform a. Both variants 3 and 4 encode isoform c. ##Evidence-Data-START## Transcript exon combination :: AK005885.1, BY706028.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAATCCTG ATGGAAAGAA CAGACAAGGT GGGAAGGAAG CTGGGAAGCA ACAGCCTGTG 
    GTGATTCTCT TGGGCTGGGG AGGCTGCAGG GACAAGAACC TGGCCAAGTA TAGTGCTATC
    TACCACAAAA GGGGCTGCAT TGTGATCCGA TACACGGCCC CCTGGCACAT GGTCTTCTTC
    TCTGAGTCCT TGGGCATCCC TTCACTTCGC GTGATAGCCC AGAAACTGCT GGAGCTCCTC
    TTTGACTATG AGATTGAGCG GGAGCCTCTG CTCTTCCACG TCTTCAGCAA TGCCGGCGTC
    ATGCTCTACC GCTACGTGCT GGAGCTCCTA CAGACCCACC AGCGCTTCCG CCATTTGCAT
    GTGGTGGGCA CCATCTTTGA CAGTGGTCCT GGCGATAGCA ACCTGATAGG GGCCCTGAGG
    GCTCTGGCAA CCATCCTGGA GCGCCGACCT GCGGTGCTGC GCCTGTTGCT CCTGGCAGCA
    TTTGCCCTGG TGGTGATCCT GTTTCACTTC CTGCTTGCTC CATTCACTGC CCTCTTCCAC
    ACCCACTTCT ACGACAGGCT GCAGGACTCA GGCTCCTGCT GGCCTGAACT CTACCTCTAC
    TCTAGAGCTG ATAAGGTGGT CTCGGCCAGG GATGTGGAAC GTATGGTAGA GGCACGCCTG
    GCTCACCAGG TCATGGTGCG TGGAGTGGAC TTTGTGTCAT CTGCGCATGT CAGCCACCTC
    CGAGACTATC CTACTTACTA TACAAGTCTG TGTGTTGACT TCATGCATAA TTGTGTCCAA
    TGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_083865.1
    CDS91..876
    Translation

    Target ORF information:

    RefSeq Version NM_029589.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus transmembrane protein 53 (Tmem53), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_029589.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAATCCTG ATGGAAAGAA CAGACAAGGT GGGAAGGAAG CTGGGAAGCA ACAGCCTGTG 
    GTGATTCTCT TGGGCTGGGG AGGCTGCAGG GACAAGAACC TGGCCAAGTA TAGTGCTATC
    TACCACAAAA GGGGCTGCAT TGTGATCCGA TACACGGCCC CCTGGCACAT GGTCTTCTTC
    TCTGAGTCCT TGGGCATCCC TTCACTTCGC GTGATAGCCC AGAAACTGCT GGAGCTCCTC
    TTTGACTATG AGATTGAGCG GGAGCCTCTG CTCTTCCACG TCTTCAGCAA TGCCGGCGTC
    ATGCTCTACC GCTACGTGCT GGAGCTCCTA CAGACCCACC AGCGCTTCCG CCATTTGCAT
    GTGGTGGGCA CCATCTTTGA CAGTGGTCCT GGCGATAGCA ACCTGATAGG GGCCCTGAGG
    GCTCTGGCAA CCATCCTGGA GCGCCGACCT GCGGTGCTGC GCCTGTTGCT CCTGGCAGCA
    TTTGCCCTGG TGGTGATCCT GTTTCACTTC CTGCTTGCTC CATTCACTGC CCTCTTCCAC
    ACCCACTTCT ACGACAGGCT GCAGGACTCA GGCTCCTGCT GGCCTGAACT CTACCTCTAC
    TCTAGAGCTG ATAAGGTGGT CTCGGCCAGG GATGTGGAAC GTATGGTAGA GGCACGCCTG
    GCTCACCAGG TCATGGTGCG TGGAGTGGAC TTTGTGTCAT CTGCGCATGT CAGCCACCTC
    CGAGACTATC CTACTTACTA TACAAGTCTG TGTGTTGACT TCATGCATAA TTGTGTCCAA
    TGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu17356
    Clone ID Related Accession (Same CDS sequence) NM_029589.1 , NM_001285814.1
    Accession Version NM_001285814.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 786bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Mus musculus(house mouse)
    Product transmembrane protein 53 isoform c
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL671866.8, AK004164.1, AW049729.1 and AL844169.6. Transcript Variant: This variant (4) differs in its 5' UTR and 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (c) has a distinct and shorter N-terminus, compared to isoform a. Both variants 3 and 4 encode isoform c. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BQ928065.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384, SAMN00849385 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAATCCTG ATGGAAAGAA CAGACAAGGT GGGAAGGAAG CTGGGAAGCA ACAGCCTGTG 
    GTGATTCTCT TGGGCTGGGG AGGCTGCAGG GACAAGAACC TGGCCAAGTA TAGTGCTATC
    TACCACAAAA GGGGCTGCAT TGTGATCCGA TACACGGCCC CCTGGCACAT GGTCTTCTTC
    TCTGAGTCCT TGGGCATCCC TTCACTTCGC GTGATAGCCC AGAAACTGCT GGAGCTCCTC
    TTTGACTATG AGATTGAGCG GGAGCCTCTG CTCTTCCACG TCTTCAGCAA TGCCGGCGTC
    ATGCTCTACC GCTACGTGCT GGAGCTCCTA CAGACCCACC AGCGCTTCCG CCATTTGCAT
    GTGGTGGGCA CCATCTTTGA CAGTGGTCCT GGCGATAGCA ACCTGATAGG GGCCCTGAGG
    GCTCTGGCAA CCATCCTGGA GCGCCGACCT GCGGTGCTGC GCCTGTTGCT CCTGGCAGCA
    TTTGCCCTGG TGGTGATCCT GTTTCACTTC CTGCTTGCTC CATTCACTGC CCTCTTCCAC
    ACCCACTTCT ACGACAGGCT GCAGGACTCA GGCTCCTGCT GGCCTGAACT CTACCTCTAC
    TCTAGAGCTG ATAAGGTGGT CTCGGCCAGG GATGTGGAAC GTATGGTAGA GGCACGCCTG
    GCTCACCAGG TCATGGTGCG TGGAGTGGAC TTTGTGTCAT CTGCGCATGT CAGCCACCTC
    CGAGACTATC CTACTTACTA TACAAGTCTG TGTGTTGACT TCATGCATAA TTGTGTCCAA
    TGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001272743.1
    CDS255..1040
    Translation

    Target ORF information:

    RefSeq Version NM_001285814.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus transmembrane protein 53 (Tmem53), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001285814.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAATCCTG ATGGAAAGAA CAGACAAGGT GGGAAGGAAG CTGGGAAGCA ACAGCCTGTG 
    GTGATTCTCT TGGGCTGGGG AGGCTGCAGG GACAAGAACC TGGCCAAGTA TAGTGCTATC
    TACCACAAAA GGGGCTGCAT TGTGATCCGA TACACGGCCC CCTGGCACAT GGTCTTCTTC
    TCTGAGTCCT TGGGCATCCC TTCACTTCGC GTGATAGCCC AGAAACTGCT GGAGCTCCTC
    TTTGACTATG AGATTGAGCG GGAGCCTCTG CTCTTCCACG TCTTCAGCAA TGCCGGCGTC
    ATGCTCTACC GCTACGTGCT GGAGCTCCTA CAGACCCACC AGCGCTTCCG CCATTTGCAT
    GTGGTGGGCA CCATCTTTGA CAGTGGTCCT GGCGATAGCA ACCTGATAGG GGCCCTGAGG
    GCTCTGGCAA CCATCCTGGA GCGCCGACCT GCGGTGCTGC GCCTGTTGCT CCTGGCAGCA
    TTTGCCCTGG TGGTGATCCT GTTTCACTTC CTGCTTGCTC CATTCACTGC CCTCTTCCAC
    ACCCACTTCT ACGACAGGCT GCAGGACTCA GGCTCCTGCT GGCCTGAACT CTACCTCTAC
    TCTAGAGCTG ATAAGGTGGT CTCGGCCAGG GATGTGGAAC GTATGGTAGA GGCACGCCTG
    GCTCACCAGG TCATGGTGCG TGGAGTGGAC TTTGTGTCAT CTGCGCATGT CAGCCACCTC
    CGAGACTATC CTACTTACTA TACAAGTCTG TGTGTTGACT TCATGCATAA TTGTGTCCAA
    TGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu03137
    Clone ID Related Accession (Same CDS sequence) NM_026837.3
    Accession Version NM_026837.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 831bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Mus musculus(house mouse)
    Product transmembrane protein 53 isoform b
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL671866.8, AK004164.1, AW049729.1 and AL844169.6. On Oct 23, 2013 this sequence version replaced NM_026837.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a. ##Evidence-Data-START## Transcript exon combination :: BI248980.1, BY704409.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGCCTCGG CCGAGCTGGA TTACAGCATC GAGATCCCGG ATCAGCCCTG CTGGAGCCAG 
    AAGAACAGAC AAGGTGGGAA GGAAGCTGGG AAGCAACAGC CTGTGGTGAT TCTCTTGGGC
    TGGGGAGGCT GCAGGGACAA GAACCTGGCC AAGTATAGTG CTATCTACCA CAAAAGGGGC
    TGCATTGTGA TCCGATACAC GGCCCCCTGG CACATGGTCT TCTTCTCTGA GTCCTTGGGC
    ATCCCTTCAC TTCGCGTGAT AGCCCAGAAA CTGCTGGAGC TCCTCTTTGA CTATGAGATT
    GAGCGGGAGC CTCTGCTCTT CCACGTCTTC AGCAATGCCG GCGTCATGCT CTACCGCTAC
    GTGCTGGAGC TCCTACAGAC CCACCAGCGC TTCCGCCATT TGCATGTGGT GGGCACCATC
    TTTGACAGTG GTCCTGGCGA TAGCAACCTG ATAGGGGCCC TGAGGGCTCT GGCAACCATC
    CTGGAGCGCC GACCTGCGGT GCTGCGCCTG TTGCTCCTGG CAGCATTTGC CCTGGTGGTG
    ATCCTGTTTC ACTTCCTGCT TGCTCCATTC ACTGCCCTCT TCCACACCCA CTTCTACGAC
    AGGCTGCAGG ACTCAGGCTC CTGCTGGCCT GAACTCTACC TCTACTCTAG AGCTGATAAG
    GTGGTCTCGG CCAGGGATGT GGAACGTATG GTAGAGGCAC GCCTGGCTCA CCAGGTCATG
    GTGCGTGGAG TGGACTTTGT GTCATCTGCG CATGTCAGCC ACCTCCGAGA CTATCCTACT
    TACTATACAA GTCTGTGTGT TGACTTCATG CATAATTGTG TCCAATGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_081113.1
    CDS124..954
    Translation

    Target ORF information:

    RefSeq Version NM_026837.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus transmembrane protein 53 (Tmem53), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_026837.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGCCTCGG CCGAGCTGGA TTACAGCATC GAGATCCCGG ATCAGCCCTG CTGGAGCCAG 
    AAGAACAGAC AAGGTGGGAA GGAAGCTGGG AAGCAACAGC CTGTGGTGAT TCTCTTGGGC
    TGGGGAGGCT GCAGGGACAA GAACCTGGCC AAGTATAGTG CTATCTACCA CAAAAGGGGC
    TGCATTGTGA TCCGATACAC GGCCCCCTGG CACATGGTCT TCTTCTCTGA GTCCTTGGGC
    ATCCCTTCAC TTCGCGTGAT AGCCCAGAAA CTGCTGGAGC TCCTCTTTGA CTATGAGATT
    GAGCGGGAGC CTCTGCTCTT CCACGTCTTC AGCAATGCCG GCGTCATGCT CTACCGCTAC
    GTGCTGGAGC TCCTACAGAC CCACCAGCGC TTCCGCCATT TGCATGTGGT GGGCACCATC
    TTTGACAGTG GTCCTGGCGA TAGCAACCTG ATAGGGGCCC TGAGGGCTCT GGCAACCATC
    CTGGAGCGCC GACCTGCGGT GCTGCGCCTG TTGCTCCTGG CAGCATTTGC CCTGGTGGTG
    ATCCTGTTTC ACTTCCTGCT TGCTCCATTC ACTGCCCTCT TCCACACCCA CTTCTACGAC
    AGGCTGCAGG ACTCAGGCTC CTGCTGGCCT GAACTCTACC TCTACTCTAG AGCTGATAAG
    GTGGTCTCGG CCAGGGATGT GGAACGTATG GTAGAGGCAC GCCTGGCTCA CCAGGTCATG
    GTGCGTGGAG TGGACTTTGT GTCATCTGCG CATGTCAGCC ACCTCCGAGA CTATCCTACT
    TACTATACAA GTCTGTGTGT TGACTTCATG CATAATTGTG TCCAATGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu08888
    Clone ID Related Accession (Same CDS sequence) NM_001285815.1 , XM_030253755.1 , NM_001285816.1
    Accession Version NM_001285815.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 618bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Mus musculus(house mouse)
    Product transmembrane protein 53 isoform d
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL671866.8, BE647557.1, AK004164.1, AW049729.1 and AL844169.6. Transcript Variant: This variant (5) differs in its 5' UTR and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (d) is shorter at the N-terminus, compared to isoform a. Both variants 5 and 6 encode isoform d. ##Evidence-Data-START## CDS exon combination :: BI248980.1, AI429067.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN00849386 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGTCTTCT TCTCTGAGTC CTTGGGCATC CCTTCACTTC GCGTGATAGC CCAGAAACTG 
    CTGGAGCTCC TCTTTGACTA TGAGATTGAG CGGGAGCCTC TGCTCTTCCA CGTCTTCAGC
    AATGCCGGCG TCATGCTCTA CCGCTACGTG CTGGAGCTCC TACAGACCCA CCAGCGCTTC
    CGCCATTTGC ATGTGGTGGG CACCATCTTT GACAGTGGTC CTGGCGATAG CAACCTGATA
    GGGGCCCTGA GGGCTCTGGC AACCATCCTG GAGCGCCGAC CTGCGGTGCT GCGCCTGTTG
    CTCCTGGCAG CATTTGCCCT GGTGGTGATC CTGTTTCACT TCCTGCTTGC TCCATTCACT
    GCCCTCTTCC ACACCCACTT CTACGACAGG CTGCAGGACT CAGGCTCCTG CTGGCCTGAA
    CTCTACCTCT ACTCTAGAGC TGATAAGGTG GTCTCGGCCA GGGATGTGGA ACGTATGGTA
    GAGGCACGCC TGGCTCACCA GGTCATGGTG CGTGGAGTGG ACTTTGTGTC ATCTGCGCAT
    GTCAGCCACC TCCGAGACTA TCCTACTTAC TATACAAGTC TGTGTGTTGA CTTCATGCAT
    AATTGTGTCC AATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001272744.1
    CDS441..1058
    Translation

    Target ORF information:

    RefSeq Version NM_001285815.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus transmembrane protein 53 (Tmem53), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001285815.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGTCTTCT TCTCTGAGTC CTTGGGCATC CCTTCACTTC GCGTGATAGC CCAGAAACTG 
    CTGGAGCTCC TCTTTGACTA TGAGATTGAG CGGGAGCCTC TGCTCTTCCA CGTCTTCAGC
    AATGCCGGCG TCATGCTCTA CCGCTACGTG CTGGAGCTCC TACAGACCCA CCAGCGCTTC
    CGCCATTTGC ATGTGGTGGG CACCATCTTT GACAGTGGTC CTGGCGATAG CAACCTGATA
    GGGGCCCTGA GGGCTCTGGC AACCATCCTG GAGCGCCGAC CTGCGGTGCT GCGCCTGTTG
    CTCCTGGCAG CATTTGCCCT GGTGGTGATC CTGTTTCACT TCCTGCTTGC TCCATTCACT
    GCCCTCTTCC ACACCCACTT CTACGACAGG CTGCAGGACT CAGGCTCCTG CTGGCCTGAA
    CTCTACCTCT ACTCTAGAGC TGATAAGGTG GTCTCGGCCA GGGATGTGGA ACGTATGGTA
    GAGGCACGCC TGGCTCACCA GGTCATGGTG CGTGGAGTGG ACTTTGTGTC ATCTGCGCAT
    GTCAGCCACC TCCGAGACTA TCCTACTTAC TATACAAGTC TGTGTGTTGA CTTCATGCAT
    AATTGTGTCC AATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu08888
    Clone ID Related Accession (Same CDS sequence) NM_001285815.1 , XM_030253755.1 , NM_001285816.1
    Accession Version NM_001285816.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 618bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Mus musculus(house mouse)
    Product transmembrane protein 53 isoform d
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL671866.8, AK004164.1, AW049729.1 and AL844169.6. Transcript Variant: This variant (6) lacks a portion of the 5' coding region and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (d) is shorter at the N-terminus, compared to isoform a. Both variants 5 and 6 encode isoform d. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BF123463.1, AA869708.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGTCTTCT TCTCTGAGTC CTTGGGCATC CCTTCACTTC GCGTGATAGC CCAGAAACTG 
    CTGGAGCTCC TCTTTGACTA TGAGATTGAG CGGGAGCCTC TGCTCTTCCA CGTCTTCAGC
    AATGCCGGCG TCATGCTCTA CCGCTACGTG CTGGAGCTCC TACAGACCCA CCAGCGCTTC
    CGCCATTTGC ATGTGGTGGG CACCATCTTT GACAGTGGTC CTGGCGATAG CAACCTGATA
    GGGGCCCTGA GGGCTCTGGC AACCATCCTG GAGCGCCGAC CTGCGGTGCT GCGCCTGTTG
    CTCCTGGCAG CATTTGCCCT GGTGGTGATC CTGTTTCACT TCCTGCTTGC TCCATTCACT
    GCCCTCTTCC ACACCCACTT CTACGACAGG CTGCAGGACT CAGGCTCCTG CTGGCCTGAA
    CTCTACCTCT ACTCTAGAGC TGATAAGGTG GTCTCGGCCA GGGATGTGGA ACGTATGGTA
    GAGGCACGCC TGGCTCACCA GGTCATGGTG CGTGGAGTGG ACTTTGTGTC ATCTGCGCAT
    GTCAGCCACC TCCGAGACTA TCCTACTTAC TATACAAGTC TGTGTGTTGA CTTCATGCAT
    AATTGTGTCC AATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001272745.1
    CDS221..838
    Translation

    Target ORF information:

    RefSeq Version NM_001285816.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus transmembrane protein 53 (Tmem53), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001285816.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGTCTTCT TCTCTGAGTC CTTGGGCATC CCTTCACTTC GCGTGATAGC CCAGAAACTG 
    CTGGAGCTCC TCTTTGACTA TGAGATTGAG CGGGAGCCTC TGCTCTTCCA CGTCTTCAGC
    AATGCCGGCG TCATGCTCTA CCGCTACGTG CTGGAGCTCC TACAGACCCA CCAGCGCTTC
    CGCCATTTGC ATGTGGTGGG CACCATCTTT GACAGTGGTC CTGGCGATAG CAACCTGATA
    GGGGCCCTGA GGGCTCTGGC AACCATCCTG GAGCGCCGAC CTGCGGTGCT GCGCCTGTTG
    CTCCTGGCAG CATTTGCCCT GGTGGTGATC CTGTTTCACT TCCTGCTTGC TCCATTCACT
    GCCCTCTTCC ACACCCACTT CTACGACAGG CTGCAGGACT CAGGCTCCTG CTGGCCTGAA
    CTCTACCTCT ACTCTAGAGC TGATAAGGTG GTCTCGGCCA GGGATGTGGA ACGTATGGTA
    GAGGCACGCC TGGCTCACCA GGTCATGGTG CGTGGAGTGG ACTTTGTGTC ATCTGCGCAT
    GTCAGCCACC TCCGAGACTA TCCTACTTAC TATACAAGTC TGTGTGTTGA CTTCATGCAT
    AATTGTGTCC AATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu08888
    Clone ID Related Accession (Same CDS sequence) NM_001285815.1 , XM_030253755.1 , NM_001285816.1
    Accession Version XM_030253755.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 618bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product transmembrane protein 53 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGTCTTCT TCTCTGAGTC CTTGGGCATC CCTTCACTTC GCGTGATAGC CCAGAAACTG 
    CTGGAGCTCC TCTTTGACTA TGAGATTGAG CGGGAGCCTC TGCTCTTCCA CGTCTTCAGC
    AATGCCGGCG TCATGCTCTA CCGCTACGTG CTGGAGCTCC TACAGACCCA CCAGCGCTTC
    CGCCATTTGC ATGTGGTGGG CACCATCTTT GACAGTGGTC CTGGCGATAG CAACCTGATA
    GGGGCCCTGA GGGCTCTGGC AACCATCCTG GAGCGCCGAC CTGCGGTGCT GCGCCTGTTG
    CTCCTGGCAG CATTTGCCCT GGTGGTGATC CTGTTTCACT TCCTGCTTGC TCCATTCACT
    GCCCTCTTCC ACACCCACTT CTACGACAGG CTGCAGGACT CAGGCTCCTG CTGGCCTGAA
    CTCTACCTCT ACTCTAGAGC TGATAAGGTG GTCTCGGCCA GGGATGTGGA ACGTATGGTA
    GAGGCACGCC TGGCTCACCA GGTCATGGTG CGTGGAGTGG ACTTTGTGTC ATCTGCGCAT
    GTCAGCCACC TCCGAGACTA TCCTACTTAC TATACAAGTC TGTGTGTTGA CTTCATGCAT
    AATTGTGTCC AATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030109615.1
    CDS355..972
    Translation

    Target ORF information:

    RefSeq Version XM_030253755.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus transmembrane protein 53 (Tmem53), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030253755.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGTCTTCT TCTCTGAGTC CTTGGGCATC CCTTCACTTC GCGTGATAGC CCAGAAACTG 
    CTGGAGCTCC TCTTTGACTA TGAGATTGAG CGGGAGCCTC TGCTCTTCCA CGTCTTCAGC
    AATGCCGGCG TCATGCTCTA CCGCTACGTG CTGGAGCTCC TACAGACCCA CCAGCGCTTC
    CGCCATTTGC ATGTGGTGGG CACCATCTTT GACAGTGGTC CTGGCGATAG CAACCTGATA
    GGGGCCCTGA GGGCTCTGGC AACCATCCTG GAGCGCCGAC CTGCGGTGCT GCGCCTGTTG
    CTCCTGGCAG CATTTGCCCT GGTGGTGATC CTGTTTCACT TCCTGCTTGC TCCATTCACT
    GCCCTCTTCC ACACCCACTT CTACGACAGG CTGCAGGACT CAGGCTCCTG CTGGCCTGAA
    CTCTACCTCT ACTCTAGAGC TGATAAGGTG GTCTCGGCCA GGGATGTGGA ACGTATGGTA
    GAGGCACGCC TGGCTCACCA GGTCATGGTG CGTGGAGTGG ACTTTGTGTC ATCTGCGCAT
    GTCAGCCACC TCCGAGACTA TCCTACTTAC TATACAAGTC TGTGTGTTGA CTTCATGCAT
    AATTGTGTCC AATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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