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Tmem53 cDNA ORF clone, Mus musculus(house mouse)

Summary

Gene Symbol Tmem53
Entrez Gene ID 68777
Full Name transmembrane protein 53
Synonyms 1110038M16Rik,1500001P22Rik,1700012A05Rik
General protein information
Preferred Names

transmembrane protein 53

Names

transmembrane protein 53
Gene Type protein-coding
Organism Mus musculus(house mouse)
Genome

4

4|4 D1

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001285815.1 NP_001272744.1 transmembrane protein 53 isoform d
NM_029589.1 NP_083865.1 transmembrane protein 53 isoform c
NM_001285812.1 NP_001272741.1 transmembrane protein 53 isoform a
NM_026837.3 NP_081113.1 transmembrane protein 53 isoform b
XM_030253755.1 XP_030109615.1 transmembrane protein 53 isoform X1
NM_001285814.1 NP_001272743.1 transmembrane protein 53 isoform c
NM_001285816.1 NP_001272745.1 transmembrane protein 53 isoform d

Pathways from BioSystems


Homologies


TMEM53 XP_002801564.1
Bos taurus (cattle) TMEM53 NP_001033661.1
Mus musculus (house mouse) Tmem53 NP_001272741.1
Rattus norvegicus (Norway rat) Tmem53 NP_001101434.1
Danio rerio (zebrafish) tmem53 NP_001002637.1
Oryza sativa (rice) Os06g0730300 NP_001058655.1
Xenopus tropicalis (tropical clawed frog) tmem53 NP_001107322.1
Caenorhabditis elegans (roundworm) C09B8.4 NP_509006.2
Pan troglodytes (chimpanzee) TMEM53 XP_513099.3
Gallus gallus (chicken) TMEM53 NP_001264509.1
Homo sapiens (human) TMEM53 NP_078863.2
Caenorhabditis elegans (roundworm) T10B11.6 NP_491980.1
Caenorhabditis elegans (roundworm) T24H10.4 NP_495945.2
Arabidopsis thaliana (thale cress) AT3G19970 NP_566652.1
Canis lupus familiaris (dog) TMEM53 XP_005629146.1
Drosophila melanogaster (fruit fly) CG8245 NP_610178.2
Anopheles gambiae (African malaria mosquito) AgaP_AGAP009586 XP_318608.3

Gene Ontology


Bibliography

Related articles in PubMed

Tissue expression patterns identify mouse cilia genes.
McClintock TS, Glasser CE, Bose SC, Bergman DA
Physiological genomics32(2)198-206(2008 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tmem53 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tmem53 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu17312 NM_001285812.1
Latest version!		 Mus musculus transmembrane protein 53 (Tmem53), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OMu17356 NM_001285814.1
Latest version!		 Mus musculus transmembrane protein 53 (Tmem53), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OMu17356 NM_029589.1
Latest version!		 Mus musculus transmembrane protein 53 (Tmem53), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OMu03137 NM_026837.3
Latest version!		 Mus musculus transmembrane protein 53 (Tmem53), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $188.30
$269.00
OMu08888 NM_001285816.1
Latest version!		 Mus musculus transmembrane protein 53 (Tmem53), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $139.30
$199.00
OMu08888 NM_001285815.1
Latest version!		 Mus musculus transmembrane protein 53 (Tmem53), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $139.30
$199.00
OMu08888 XM_030253755.1
Latest version!		 Mus musculus transmembrane protein 53 (Tmem53), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $139.30
$199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OMu17312
Clone ID Related Accession (Same CDS sequence) NM_001285812.1
Accession Version NM_001285812.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 852bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-02-23
Organism Mus musculus(house mouse)
Product transmembrane protein 53 isoform a
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL671866.8, AK004164.1, AW049729.1 and AL844169.6. Transcript Variant: This variant (1) encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC020121.1, BF164608.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCCTCGG CCGAGCTGGA TTACAGCATC GAGATCCCGG ATCAGCCCTG CTGGAGCCAG 
AGTATGGAGG GGGATTGGGA AGAGAACAGA CAAGGTGGGA AGGAAGCTGG GAAGCAACAG 
CCTGTGGTGA TTCTCTTGGG CTGGGGAGGC TGCAGGGACA AGAACCTGGC CAAGTATAGT 
GCTATCTACC ACAAAAGGGG CTGCATTGTG ATCCGATACA CGGCCCCCTG GCACATGGTC 
TTCTTCTCTG AGTCCTTGGG CATCCCTTCA CTTCGCGTGA TAGCCCAGAA ACTGCTGGAG 
CTCCTCTTTG ACTATGAGAT TGAGCGGGAG CCTCTGCTCT TCCACGTCTT CAGCAATGCC 
GGCGTCATGC TCTACCGCTA CGTGCTGGAG CTCCTACAGA CCCACCAGCG CTTCCGCCAT 
TTGCATGTGG TGGGCACCAT CTTTGACAGT GGTCCTGGCG ATAGCAACCT GATAGGGGCC 
CTGAGGGCTC TGGCAACCAT CCTGGAGCGC CGACCTGCGG TGCTGCGCCT GTTGCTCCTG 
GCAGCATTTG CCCTGGTGGT GATCCTGTTT CACTTCCTGC TTGCTCCATT CACTGCCCTC 
TTCCACACCC ACTTCTACGA CAGGCTGCAG GACTCAGGCT CCTGCTGGCC TGAACTCTAC 
CTCTACTCTA GAGCTGATAA GGTGGTCTCG GCCAGGGATG TGGAACGTAT GGTAGAGGCA 
CGCCTGGCTC ACCAGGTCAT GGTGCGTGGA GTGGACTTTG TGTCATCTGC GCATGTCAGC 
CACCTCCGAG ACTATCCTAC TTACTATACA AGTCTGTGTG TTGACTTCAT GCATAATTGT 
GTCCAATGCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001272741.1
CDS124..975
Translation

Target ORF information:

RefSeq Version NM_001285812.1
Organism Mus musculus(house mouse)
Definition Mus musculus transmembrane protein 53 (Tmem53), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001285812.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCCTCGG CCGAGCTGGA TTACAGCATC GAGATCCCGG ATCAGCCCTG CTGGAGCCAG 
AGTATGGAGG GGGATTGGGA AGAGAACAGA CAAGGTGGGA AGGAAGCTGG GAAGCAACAG 
CCTGTGGTGA TTCTCTTGGG CTGGGGAGGC TGCAGGGACA AGAACCTGGC CAAGTATAGT 
GCTATCTACC ACAAAAGGGG CTGCATTGTG ATCCGATACA CGGCCCCCTG GCACATGGTC 
TTCTTCTCTG AGTCCTTGGG CATCCCTTCA CTTCGCGTGA TAGCCCAGAA ACTGCTGGAG 
CTCCTCTTTG ACTATGAGAT TGAGCGGGAG CCTCTGCTCT TCCACGTCTT CAGCAATGCC 
GGCGTCATGC TCTACCGCTA CGTGCTGGAG CTCCTACAGA CCCACCAGCG CTTCCGCCAT 
TTGCATGTGG TGGGCACCAT CTTTGACAGT GGTCCTGGCG ATAGCAACCT GATAGGGGCC 
CTGAGGGCTC TGGCAACCAT CCTGGAGCGC CGACCTGCGG TGCTGCGCCT GTTGCTCCTG 
GCAGCATTTG CCCTGGTGGT GATCCTGTTT CACTTCCTGC TTGCTCCATT CACTGCCCTC 
TTCCACACCC ACTTCTACGA CAGGCTGCAG GACTCAGGCT CCTGCTGGCC TGAACTCTAC 
CTCTACTCTA GAGCTGATAA GGTGGTCTCG GCCAGGGATG TGGAACGTAT GGTAGAGGCA 
CGCCTGGCTC ACCAGGTCAT GGTGCGTGGA GTGGACTTTG TGTCATCTGC GCATGTCAGC 
CACCTCCGAG ACTATCCTAC TTACTATACA AGTCTGTGTG TTGACTTCAT GCATAATTGT 
GTCCAATGCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu17356
Clone ID Related Accession (Same CDS sequence) NM_029589.1 , NM_001285814.1
Accession Version NM_029589.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 786bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-02-23
Organism Mus musculus(house mouse)
Product transmembrane protein 53 isoform c
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK005885.1, AK005104.1 and AL844169.6. Transcript Variant: This variant (3) includes alternate 5' exon structure and it thus differs in its 5' UTR and 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (c) has a distinct and shorter N-terminus, compared to isoform a. Both variants 3 and 4 encode isoform c. ##Evidence-Data-START## Transcript exon combination :: AK005885.1, BY706028.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGAATCCTG ATGGAAAGAA CAGACAAGGT GGGAAGGAAG CTGGGAAGCA ACAGCCTGTG 
GTGATTCTCT TGGGCTGGGG AGGCTGCAGG GACAAGAACC TGGCCAAGTA TAGTGCTATC 
TACCACAAAA GGGGCTGCAT TGTGATCCGA TACACGGCCC CCTGGCACAT GGTCTTCTTC 
TCTGAGTCCT TGGGCATCCC TTCACTTCGC GTGATAGCCC AGAAACTGCT GGAGCTCCTC 
TTTGACTATG AGATTGAGCG GGAGCCTCTG CTCTTCCACG TCTTCAGCAA TGCCGGCGTC 
ATGCTCTACC GCTACGTGCT GGAGCTCCTA CAGACCCACC AGCGCTTCCG CCATTTGCAT 
GTGGTGGGCA CCATCTTTGA CAGTGGTCCT GGCGATAGCA ACCTGATAGG GGCCCTGAGG 
GCTCTGGCAA CCATCCTGGA GCGCCGACCT GCGGTGCTGC GCCTGTTGCT CCTGGCAGCA 
TTTGCCCTGG TGGTGATCCT GTTTCACTTC CTGCTTGCTC CATTCACTGC CCTCTTCCAC 
ACCCACTTCT ACGACAGGCT GCAGGACTCA GGCTCCTGCT GGCCTGAACT CTACCTCTAC 
TCTAGAGCTG ATAAGGTGGT CTCGGCCAGG GATGTGGAAC GTATGGTAGA GGCACGCCTG 
GCTCACCAGG TCATGGTGCG TGGAGTGGAC TTTGTGTCAT CTGCGCATGT CAGCCACCTC 
CGAGACTATC CTACTTACTA TACAAGTCTG TGTGTTGACT TCATGCATAA TTGTGTCCAA 
TGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_083865.1
CDS91..876
Translation

Target ORF information:

RefSeq Version NM_029589.1
Organism Mus musculus(house mouse)
Definition Mus musculus transmembrane protein 53 (Tmem53), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_029589.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGAATCCTG ATGGAAAGAA CAGACAAGGT GGGAAGGAAG CTGGGAAGCA ACAGCCTGTG 
GTGATTCTCT TGGGCTGGGG AGGCTGCAGG GACAAGAACC TGGCCAAGTA TAGTGCTATC 
TACCACAAAA GGGGCTGCAT TGTGATCCGA TACACGGCCC CCTGGCACAT GGTCTTCTTC 
TCTGAGTCCT TGGGCATCCC TTCACTTCGC GTGATAGCCC AGAAACTGCT GGAGCTCCTC 
TTTGACTATG AGATTGAGCG GGAGCCTCTG CTCTTCCACG TCTTCAGCAA TGCCGGCGTC 
ATGCTCTACC GCTACGTGCT GGAGCTCCTA CAGACCCACC AGCGCTTCCG CCATTTGCAT 
GTGGTGGGCA CCATCTTTGA CAGTGGTCCT GGCGATAGCA ACCTGATAGG GGCCCTGAGG 
GCTCTGGCAA CCATCCTGGA GCGCCGACCT GCGGTGCTGC GCCTGTTGCT CCTGGCAGCA 
TTTGCCCTGG TGGTGATCCT GTTTCACTTC CTGCTTGCTC CATTCACTGC CCTCTTCCAC 
ACCCACTTCT ACGACAGGCT GCAGGACTCA GGCTCCTGCT GGCCTGAACT CTACCTCTAC 
TCTAGAGCTG ATAAGGTGGT CTCGGCCAGG GATGTGGAAC GTATGGTAGA GGCACGCCTG 
GCTCACCAGG TCATGGTGCG TGGAGTGGAC TTTGTGTCAT CTGCGCATGT CAGCCACCTC 
CGAGACTATC CTACTTACTA TACAAGTCTG TGTGTTGACT TCATGCATAA TTGTGTCCAA 
TGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu17356
Clone ID Related Accession (Same CDS sequence) NM_029589.1 , NM_001285814.1
Accession Version NM_001285814.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 786bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-02-23
Organism Mus musculus(house mouse)
Product transmembrane protein 53 isoform c
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL671866.8, AK004164.1, AW049729.1 and AL844169.6. Transcript Variant: This variant (4) differs in its 5' UTR and 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (c) has a distinct and shorter N-terminus, compared to isoform a. Both variants 3 and 4 encode isoform c. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BQ928065.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384, SAMN00849385 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGAATCCTG ATGGAAAGAA CAGACAAGGT GGGAAGGAAG CTGGGAAGCA ACAGCCTGTG 
GTGATTCTCT TGGGCTGGGG AGGCTGCAGG GACAAGAACC TGGCCAAGTA TAGTGCTATC 
TACCACAAAA GGGGCTGCAT TGTGATCCGA TACACGGCCC CCTGGCACAT GGTCTTCTTC 
TCTGAGTCCT TGGGCATCCC TTCACTTCGC GTGATAGCCC AGAAACTGCT GGAGCTCCTC 
TTTGACTATG AGATTGAGCG GGAGCCTCTG CTCTTCCACG TCTTCAGCAA TGCCGGCGTC 
ATGCTCTACC GCTACGTGCT GGAGCTCCTA CAGACCCACC AGCGCTTCCG CCATTTGCAT 
GTGGTGGGCA CCATCTTTGA CAGTGGTCCT GGCGATAGCA ACCTGATAGG GGCCCTGAGG 
GCTCTGGCAA CCATCCTGGA GCGCCGACCT GCGGTGCTGC GCCTGTTGCT CCTGGCAGCA 
TTTGCCCTGG TGGTGATCCT GTTTCACTTC CTGCTTGCTC CATTCACTGC CCTCTTCCAC 
ACCCACTTCT ACGACAGGCT GCAGGACTCA GGCTCCTGCT GGCCTGAACT CTACCTCTAC 
TCTAGAGCTG ATAAGGTGGT CTCGGCCAGG GATGTGGAAC GTATGGTAGA GGCACGCCTG 
GCTCACCAGG TCATGGTGCG TGGAGTGGAC TTTGTGTCAT CTGCGCATGT CAGCCACCTC 
CGAGACTATC CTACTTACTA TACAAGTCTG TGTGTTGACT TCATGCATAA TTGTGTCCAA 
TGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001272743.1
CDS255..1040
Translation

Target ORF information:

RefSeq Version NM_001285814.1
Organism Mus musculus(house mouse)
Definition Mus musculus transmembrane protein 53 (Tmem53), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001285814.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGAATCCTG ATGGAAAGAA CAGACAAGGT GGGAAGGAAG CTGGGAAGCA ACAGCCTGTG 
GTGATTCTCT TGGGCTGGGG AGGCTGCAGG GACAAGAACC TGGCCAAGTA TAGTGCTATC 
TACCACAAAA GGGGCTGCAT TGTGATCCGA TACACGGCCC CCTGGCACAT GGTCTTCTTC 
TCTGAGTCCT TGGGCATCCC TTCACTTCGC GTGATAGCCC AGAAACTGCT GGAGCTCCTC 
TTTGACTATG AGATTGAGCG GGAGCCTCTG CTCTTCCACG TCTTCAGCAA TGCCGGCGTC 
ATGCTCTACC GCTACGTGCT GGAGCTCCTA CAGACCCACC AGCGCTTCCG CCATTTGCAT 
GTGGTGGGCA CCATCTTTGA CAGTGGTCCT GGCGATAGCA ACCTGATAGG GGCCCTGAGG 
GCTCTGGCAA CCATCCTGGA GCGCCGACCT GCGGTGCTGC GCCTGTTGCT CCTGGCAGCA 
TTTGCCCTGG TGGTGATCCT GTTTCACTTC CTGCTTGCTC CATTCACTGC CCTCTTCCAC 
ACCCACTTCT ACGACAGGCT GCAGGACTCA GGCTCCTGCT GGCCTGAACT CTACCTCTAC 
TCTAGAGCTG ATAAGGTGGT CTCGGCCAGG GATGTGGAAC GTATGGTAGA GGCACGCCTG 
GCTCACCAGG TCATGGTGCG TGGAGTGGAC TTTGTGTCAT CTGCGCATGT CAGCCACCTC 
CGAGACTATC CTACTTACTA TACAAGTCTG TGTGTTGACT TCATGCATAA TTGTGTCCAA 
TGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu03137
Clone ID Related Accession (Same CDS sequence) NM_026837.3
Accession Version NM_026837.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 831bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-02-23
Organism Mus musculus(house mouse)
Product transmembrane protein 53 isoform b
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL671866.8, AK004164.1, AW049729.1 and AL844169.6. On Oct 23, 2013 this sequence version replaced NM_026837.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a. ##Evidence-Data-START## Transcript exon combination :: BI248980.1, BY704409.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCCTCGG CCGAGCTGGA TTACAGCATC GAGATCCCGG ATCAGCCCTG CTGGAGCCAG 
AAGAACAGAC AAGGTGGGAA GGAAGCTGGG AAGCAACAGC CTGTGGTGAT TCTCTTGGGC 
TGGGGAGGCT GCAGGGACAA GAACCTGGCC AAGTATAGTG CTATCTACCA CAAAAGGGGC 
TGCATTGTGA TCCGATACAC GGCCCCCTGG CACATGGTCT TCTTCTCTGA GTCCTTGGGC 
ATCCCTTCAC TTCGCGTGAT AGCCCAGAAA CTGCTGGAGC TCCTCTTTGA CTATGAGATT 
GAGCGGGAGC CTCTGCTCTT CCACGTCTTC AGCAATGCCG GCGTCATGCT CTACCGCTAC 
GTGCTGGAGC TCCTACAGAC CCACCAGCGC TTCCGCCATT TGCATGTGGT GGGCACCATC 
TTTGACAGTG GTCCTGGCGA TAGCAACCTG ATAGGGGCCC TGAGGGCTCT GGCAACCATC 
CTGGAGCGCC GACCTGCGGT GCTGCGCCTG TTGCTCCTGG CAGCATTTGC CCTGGTGGTG 
ATCCTGTTTC ACTTCCTGCT TGCTCCATTC ACTGCCCTCT TCCACACCCA CTTCTACGAC 
AGGCTGCAGG ACTCAGGCTC CTGCTGGCCT GAACTCTACC TCTACTCTAG AGCTGATAAG 
GTGGTCTCGG CCAGGGATGT GGAACGTATG GTAGAGGCAC GCCTGGCTCA CCAGGTCATG 
GTGCGTGGAG TGGACTTTGT GTCATCTGCG CATGTCAGCC ACCTCCGAGA CTATCCTACT 
TACTATACAA GTCTGTGTGT TGACTTCATG CATAATTGTG TCCAATGCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_081113.1
CDS124..954
Translation

Target ORF information:

RefSeq Version NM_026837.3
Organism Mus musculus(house mouse)
Definition Mus musculus transmembrane protein 53 (Tmem53), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_026837.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCCTCGG CCGAGCTGGA TTACAGCATC GAGATCCCGG ATCAGCCCTG CTGGAGCCAG 
AAGAACAGAC AAGGTGGGAA GGAAGCTGGG AAGCAACAGC CTGTGGTGAT TCTCTTGGGC 
TGGGGAGGCT GCAGGGACAA GAACCTGGCC AAGTATAGTG CTATCTACCA CAAAAGGGGC 
TGCATTGTGA TCCGATACAC GGCCCCCTGG CACATGGTCT TCTTCTCTGA GTCCTTGGGC 
ATCCCTTCAC TTCGCGTGAT AGCCCAGAAA CTGCTGGAGC TCCTCTTTGA CTATGAGATT 
GAGCGGGAGC CTCTGCTCTT CCACGTCTTC AGCAATGCCG GCGTCATGCT CTACCGCTAC 
GTGCTGGAGC TCCTACAGAC CCACCAGCGC TTCCGCCATT TGCATGTGGT GGGCACCATC 
TTTGACAGTG GTCCTGGCGA TAGCAACCTG ATAGGGGCCC TGAGGGCTCT GGCAACCATC 
CTGGAGCGCC GACCTGCGGT GCTGCGCCTG TTGCTCCTGG CAGCATTTGC CCTGGTGGTG 
ATCCTGTTTC ACTTCCTGCT TGCTCCATTC ACTGCCCTCT TCCACACCCA CTTCTACGAC 
AGGCTGCAGG ACTCAGGCTC CTGCTGGCCT GAACTCTACC TCTACTCTAG AGCTGATAAG 
GTGGTCTCGG CCAGGGATGT GGAACGTATG GTAGAGGCAC GCCTGGCTCA CCAGGTCATG 
GTGCGTGGAG TGGACTTTGT GTCATCTGCG CATGTCAGCC ACCTCCGAGA CTATCCTACT 
TACTATACAA GTCTGTGTGT TGACTTCATG CATAATTGTG TCCAATGCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu08888
Clone ID Related Accession (Same CDS sequence) NM_001285815.1 , XM_030253755.1 , NM_001285816.1
Accession Version NM_001285815.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 618bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-02-23
Organism Mus musculus(house mouse)
Product transmembrane protein 53 isoform d
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL671866.8, BE647557.1, AK004164.1, AW049729.1 and AL844169.6. Transcript Variant: This variant (5) differs in its 5' UTR and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (d) is shorter at the N-terminus, compared to isoform a. Both variants 5 and 6 encode isoform d. ##Evidence-Data-START## CDS exon combination :: BI248980.1, AI429067.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN00849386 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
ATGGTCTTCT TCTCTGAGTC CTTGGGCATC CCTTCACTTC GCGTGATAGC CCAGAAACTG 
CTGGAGCTCC TCTTTGACTA TGAGATTGAG CGGGAGCCTC TGCTCTTCCA CGTCTTCAGC 
AATGCCGGCG TCATGCTCTA CCGCTACGTG CTGGAGCTCC TACAGACCCA CCAGCGCTTC 
CGCCATTTGC ATGTGGTGGG CACCATCTTT GACAGTGGTC CTGGCGATAG CAACCTGATA 
GGGGCCCTGA GGGCTCTGGC AACCATCCTG GAGCGCCGAC CTGCGGTGCT GCGCCTGTTG 
CTCCTGGCAG CATTTGCCCT GGTGGTGATC CTGTTTCACT TCCTGCTTGC TCCATTCACT 
GCCCTCTTCC ACACCCACTT CTACGACAGG CTGCAGGACT CAGGCTCCTG CTGGCCTGAA 
CTCTACCTCT ACTCTAGAGC TGATAAGGTG GTCTCGGCCA GGGATGTGGA ACGTATGGTA 
GAGGCACGCC TGGCTCACCA GGTCATGGTG CGTGGAGTGG ACTTTGTGTC ATCTGCGCAT 
GTCAGCCACC TCCGAGACTA TCCTACTTAC TATACAAGTC TGTGTGTTGA CTTCATGCAT 
AATTGTGTCC AATGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001272744.1
CDS441..1058
Translation

Target ORF information:

RefSeq Version NM_001285815.1
Organism Mus musculus(house mouse)
Definition Mus musculus transmembrane protein 53 (Tmem53), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001285815.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGTCTTCT TCTCTGAGTC CTTGGGCATC CCTTCACTTC GCGTGATAGC CCAGAAACTG 
CTGGAGCTCC TCTTTGACTA TGAGATTGAG CGGGAGCCTC TGCTCTTCCA CGTCTTCAGC 
AATGCCGGCG TCATGCTCTA CCGCTACGTG CTGGAGCTCC TACAGACCCA CCAGCGCTTC 
CGCCATTTGC ATGTGGTGGG CACCATCTTT GACAGTGGTC CTGGCGATAG CAACCTGATA 
GGGGCCCTGA GGGCTCTGGC AACCATCCTG GAGCGCCGAC CTGCGGTGCT GCGCCTGTTG 
CTCCTGGCAG CATTTGCCCT GGTGGTGATC CTGTTTCACT TCCTGCTTGC TCCATTCACT 
GCCCTCTTCC ACACCCACTT CTACGACAGG CTGCAGGACT CAGGCTCCTG CTGGCCTGAA 
CTCTACCTCT ACTCTAGAGC TGATAAGGTG GTCTCGGCCA GGGATGTGGA ACGTATGGTA 
GAGGCACGCC TGGCTCACCA GGTCATGGTG CGTGGAGTGG ACTTTGTGTC ATCTGCGCAT 
GTCAGCCACC TCCGAGACTA TCCTACTTAC TATACAAGTC TGTGTGTTGA CTTCATGCAT 
AATTGTGTCC AATGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu08888
Clone ID Related Accession (Same CDS sequence) NM_001285815.1 , XM_030253755.1 , NM_001285816.1
Accession Version NM_001285816.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 618bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-02-23
Organism Mus musculus(house mouse)
Product transmembrane protein 53 isoform d
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL671866.8, AK004164.1, AW049729.1 and AL844169.6. Transcript Variant: This variant (6) lacks a portion of the 5' coding region and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (d) is shorter at the N-terminus, compared to isoform a. Both variants 5 and 6 encode isoform d. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BF123463.1, AA869708.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
ATGGTCTTCT TCTCTGAGTC CTTGGGCATC CCTTCACTTC GCGTGATAGC CCAGAAACTG 
CTGGAGCTCC TCTTTGACTA TGAGATTGAG CGGGAGCCTC TGCTCTTCCA CGTCTTCAGC 
AATGCCGGCG TCATGCTCTA CCGCTACGTG CTGGAGCTCC TACAGACCCA CCAGCGCTTC 
CGCCATTTGC ATGTGGTGGG CACCATCTTT GACAGTGGTC CTGGCGATAG CAACCTGATA 
GGGGCCCTGA GGGCTCTGGC AACCATCCTG GAGCGCCGAC CTGCGGTGCT GCGCCTGTTG 
CTCCTGGCAG CATTTGCCCT GGTGGTGATC CTGTTTCACT TCCTGCTTGC TCCATTCACT 
GCCCTCTTCC ACACCCACTT CTACGACAGG CTGCAGGACT CAGGCTCCTG CTGGCCTGAA 
CTCTACCTCT ACTCTAGAGC TGATAAGGTG GTCTCGGCCA GGGATGTGGA ACGTATGGTA 
GAGGCACGCC TGGCTCACCA GGTCATGGTG CGTGGAGTGG ACTTTGTGTC ATCTGCGCAT 
GTCAGCCACC TCCGAGACTA TCCTACTTAC TATACAAGTC TGTGTGTTGA CTTCATGCAT 
AATTGTGTCC AATGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001272745.1
CDS221..838
Translation

Target ORF information:

RefSeq Version NM_001285816.1
Organism Mus musculus(house mouse)
Definition Mus musculus transmembrane protein 53 (Tmem53), transcript variant 6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001285816.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGTCTTCT TCTCTGAGTC CTTGGGCATC CCTTCACTTC GCGTGATAGC CCAGAAACTG 
CTGGAGCTCC TCTTTGACTA TGAGATTGAG CGGGAGCCTC TGCTCTTCCA CGTCTTCAGC 
AATGCCGGCG TCATGCTCTA CCGCTACGTG CTGGAGCTCC TACAGACCCA CCAGCGCTTC 
CGCCATTTGC ATGTGGTGGG CACCATCTTT GACAGTGGTC CTGGCGATAG CAACCTGATA 
GGGGCCCTGA GGGCTCTGGC AACCATCCTG GAGCGCCGAC CTGCGGTGCT GCGCCTGTTG 
CTCCTGGCAG CATTTGCCCT GGTGGTGATC CTGTTTCACT TCCTGCTTGC TCCATTCACT 
GCCCTCTTCC ACACCCACTT CTACGACAGG CTGCAGGACT CAGGCTCCTG CTGGCCTGAA 
CTCTACCTCT ACTCTAGAGC TGATAAGGTG GTCTCGGCCA GGGATGTGGA ACGTATGGTA 
GAGGCACGCC TGGCTCACCA GGTCATGGTG CGTGGAGTGG ACTTTGTGTC ATCTGCGCAT 
GTCAGCCACC TCCGAGACTA TCCTACTTAC TATACAAGTC TGTGTGTTGA CTTCATGCAT 
AATTGTGTCC AATGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu08888
Clone ID Related Accession (Same CDS sequence) NM_001285815.1 , XM_030253755.1 , NM_001285816.1
Accession Version XM_030253755.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 618bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product transmembrane protein 53 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
ATGGTCTTCT TCTCTGAGTC CTTGGGCATC CCTTCACTTC GCGTGATAGC CCAGAAACTG 
CTGGAGCTCC TCTTTGACTA TGAGATTGAG CGGGAGCCTC TGCTCTTCCA CGTCTTCAGC 
AATGCCGGCG TCATGCTCTA CCGCTACGTG CTGGAGCTCC TACAGACCCA CCAGCGCTTC 
CGCCATTTGC ATGTGGTGGG CACCATCTTT GACAGTGGTC CTGGCGATAG CAACCTGATA 
GGGGCCCTGA GGGCTCTGGC AACCATCCTG GAGCGCCGAC CTGCGGTGCT GCGCCTGTTG 
CTCCTGGCAG CATTTGCCCT GGTGGTGATC CTGTTTCACT TCCTGCTTGC TCCATTCACT 
GCCCTCTTCC ACACCCACTT CTACGACAGG CTGCAGGACT CAGGCTCCTG CTGGCCTGAA 
CTCTACCTCT ACTCTAGAGC TGATAAGGTG GTCTCGGCCA GGGATGTGGA ACGTATGGTA 
GAGGCACGCC TGGCTCACCA GGTCATGGTG CGTGGAGTGG ACTTTGTGTC ATCTGCGCAT 
GTCAGCCACC TCCGAGACTA TCCTACTTAC TATACAAGTC TGTGTGTTGA CTTCATGCAT 
AATTGTGTCC AATGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_030109615.1
CDS355..972
Translation

Target ORF information:

RefSeq Version XM_030253755.1
Organism Mus musculus(house mouse)
Definition Mus musculus transmembrane protein 53 (Tmem53), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_030253755.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGTCTTCT TCTCTGAGTC CTTGGGCATC CCTTCACTTC GCGTGATAGC CCAGAAACTG 
CTGGAGCTCC TCTTTGACTA TGAGATTGAG CGGGAGCCTC TGCTCTTCCA CGTCTTCAGC 
AATGCCGGCG TCATGCTCTA CCGCTACGTG CTGGAGCTCC TACAGACCCA CCAGCGCTTC 
CGCCATTTGC ATGTGGTGGG CACCATCTTT GACAGTGGTC CTGGCGATAG CAACCTGATA 
GGGGCCCTGA GGGCTCTGGC AACCATCCTG GAGCGCCGAC CTGCGGTGCT GCGCCTGTTG 
CTCCTGGCAG CATTTGCCCT GGTGGTGATC CTGTTTCACT TCCTGCTTGC TCCATTCACT 
GCCCTCTTCC ACACCCACTT CTACGACAGG CTGCAGGACT CAGGCTCCTG CTGGCCTGAA 
CTCTACCTCT ACTCTAGAGC TGATAAGGTG GTCTCGGCCA GGGATGTGGA ACGTATGGTA 
GAGGCACGCC TGGCTCACCA GGTCATGGTG CGTGGAGTGG ACTTTGTGTC ATCTGCGCAT 
GTCAGCCACC TCCGAGACTA TCCTACTTAC TATACAAGTC TGTGTGTTGA CTTCATGCAT 
AATTGTGTCC AATGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Tissue expression patterns identify mouse cilia genes.
Physiological genomics32(2)198-206(2008 Jan)
McClintock TS,Glasser CE,Bose SC,Bergman DA