The following TMEM53 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TMEM53 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY108001.1, BC064520.1 and BM675039.1. On Jul 11, 2014 this sequence version replaced NM_024587.2.
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
##Evidence-Data-START##
RNAseq introns :: single sample supports all introns SAMEA2153307, SAMEA2154665 [ECO:0000348]
##Evidence-Data-END##
COMPLETENESS: complete on the 3' end.
Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY108001.1, BC064520.1 and BM675039.1. On Nov 22, 2018 this sequence version replaced NM_024587.3.
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
##Evidence-Data-START##
Transcript exon combination :: SRR1803617.147921.1, SRR1660809.150841.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns SAMEA2153307, SAMEA2154665 [ECO:0000348]
##Evidence-Data-END##
##RefSeq-Attributes-START##
MANE Ensembl match :: ENST00000372237.8/ ENSP00000361311.3
RefSeq Select criteria :: based on conservation, expression, longest protein
##RefSeq-Attributes-END##
COMPLETENESS: full length.
Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process
On Jun 6, 2016 this sequence version replaced XM_011542138.1.
##Genome-Annotation-Data-START##
Annotation Provider :: NCBI
Annotation Status :: Updated annotation
Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205
Annotation Version :: 109.20191205
Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline
Annotation Software Version :: 8.3
Annotation Method :: Best-placed RefSeq; propagated RefSeq model
Features Annotated :: Gene; mRNA; CDS; ncRNA
##Genome-Annotation-Data-END##
Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY108001.1, HY029536.1, BC064520.1 and BM675039.1. On Jul 11, 2014 this sequence version replaced XM_005271194.2.
Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (3) is shorter than isoform 1.
##Evidence-Data-START##
Transcript exon combination :: SRR1660809.246850.1, SRR1660805.350938.1 [ECO:0000332]
RNAseq introns :: mixed/partial sample support SAMEA2153307, SAMEA2154665 [ECO:0000350]
##Evidence-Data-END##
COMPLETENESS: complete on the 3' end.
Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY108001.1, HY029536.1, BC064520.1 and BM675039.1. On Jun 1, 2019 this sequence version replaced NM_001300747.1.
Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (3) is shorter than isoform 1.
##Evidence-Data-START##
Transcript exon combination :: SRR1660809.246850.1, SRR1660805.350938.1 [ECO:0000332]
RNAseq introns :: mixed/partial sample support SAMEA2153307, SAMEA2154665 [ECO:0000350]
##Evidence-Data-END##
COMPLETENESS: complete on the 3' end.
Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY108001.1, AA490195.1, BC064520.1 and BM675039.1. On May 31, 2019 this sequence version replaced NM_001300748.1.
Transcript Variant: This variant (4) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (4) is shorter than isoform 1.
##Evidence-Data-START##
Transcript exon combination :: SRR1163655.355435.1, SRR1163657.399564.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns SAMEA2153307, SAMEA2154665 [ECO:0000348]
##Evidence-Data-END##
COMPLETENESS: complete on the 3' end.
Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY108001.1, AA490195.1, BC064520.1 and BM675039.1. On Jul 11, 2014 this sequence version replaced XM_005271195.2.
Transcript Variant: This variant (4) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (4) is shorter than isoform 1.
##Evidence-Data-START##
Transcript exon combination :: SRR1163655.355435.1, SRR1163657.399564.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns SAMEA2153307, SAMEA2154665 [ECO:0000348]
##Evidence-Data-END##
COMPLETENESS: complete on the 3' end.
Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY108001.1, AL832539.1 and BM675039.1. On Jul 11, 2014 this sequence version replaced XM_005271196.2.
Transcript Variant: This variant (2) lacks an alternate coding exon and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1.
##Evidence-Data-START##
Transcript exon combination :: SRR1803614.277949.1, AL832539.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348]
##Evidence-Data-END##
COMPLETENESS: complete on the 3' end.