TMEM53 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Gene Symbol	TMEM53
Entrez Gene ID	79639
Full Name	transmembrane protein 53
Synonyms	NET4
General protein information	Preferred Names transmembrane protein 53 Names transmembrane protein 53 novel DUF829 domain-containing protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p34.1

mRNA and Protein(s)

mRNA	Protein	Name
NM_024587.3	NP_078863.2	transmembrane protein 53 isoform 1
NM_001300747.1	NP_001287676.1	transmembrane protein 53 isoform 3
XM_011542138.2	XP_011540440.1	transmembrane protein 53 isoform X1
NM_001300747.2	NP_001287676.1	transmembrane protein 53 isoform 3
NM_001300748.2	NP_001287677.1	transmembrane protein 53 isoform 4
NM_001300748.1	NP_001287677.1	transmembrane protein 53 isoform 4
NM_024587.4	NP_078863.2	transmembrane protein 53 isoform 1
NM_001300746.1	NP_001287675.1	transmembrane protein 53 isoform 2

Pathways from BioSystems

Homologies

	TMEM53	XP_002801564.1
Bos taurus (cattle)	TMEM53	NP_001033661.1
Mus musculus (house mouse)	Tmem53	NP_001272741.1
Rattus norvegicus (Norway rat)	Tmem53	NP_001101434.1
Danio rerio (zebrafish)	tmem53	NP_001002637.1
Oryza sativa (rice)	Os06g0730300	NP_001058655.1
Xenopus tropicalis (tropical clawed frog)	tmem53	NP_001107322.1
Caenorhabditis elegans (roundworm)	C09B8.4	NP_509006.2
Pan troglodytes (chimpanzee)	TMEM53	XP_513099.3
Gallus gallus (chicken)	TMEM53	NP_001264509.1
Homo sapiens (human)	TMEM53	NP_078863.2
Caenorhabditis elegans (roundworm)	T10B11.6	NP_491980.1
Caenorhabditis elegans (roundworm)	T24H10.4	NP_495945.2
Arabidopsis thaliana (thale cress)	AT3G19970	NP_566652.1
Canis lupus familiaris (dog)	TMEM53	XP_005629146.1
Drosophila melanogaster (fruit fly)	CG8245	NP_610178.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009586	XP_318608.3

Gene Ontology
Bibliography

Related articles in PubMed

Proteomic characterization of the human sperm nucleus.
de Mateo S, Castillo J, Estanyol JM, Ballesc? JL, Oliva R
Proteomics11(13)2714-26(2011 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TMEM53 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TMEM53 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13124	NM_024587.4 Latest version!	Homo sapiens transmembrane protein 53 (TMEM53), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu71957	XM_011542138.2 Latest version!	Homo sapiens transmembrane protein 53 (TMEM53), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu33798	NM_001300747.2 Latest version!	Homo sapiens transmembrane protein 53 (TMEM53), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu33799	NM_001300748.2 Latest version!	Homo sapiens transmembrane protein 53 (TMEM53), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu33800	NM_001300746.1 Latest version!	Homo sapiens transmembrane protein 53 (TMEM53), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
View Old Accession Versions >>
OHu33798	NM_001300747.1	Homo sapiens transmembrane protein 53 (TMEM53), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu33799	NM_001300748.1	Homo sapiens transmembrane protein 53 (TMEM53), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu13124	NM_024587.3	Homo sapiens transmembrane protein 53 (TMEM53), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TMEM53 ( NM_024587.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_078863.2 Homo sapiens transmembrane protein 53 (TMEM53), transcript variant 1, mRNA.

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CloneID	OHu13124
Clone ID Related Accession (Same CDS sequence)	NM_024587.3 , NM_024587.4
Accession Version	NM_024587.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	transmembrane protein 53 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY108001.1, BC064520.1 and BM675039.1. On Jul 11, 2014 this sequence version replaced NM_024587.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2153307, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTCGG CAGAGCTGGA CTACACCATC GAGATCCCGG ATCAGCCCTG CTGGAGCCAG 
AAGAACAGCC CCAGCCCAGG TGGGAAGGAG GCAGAAACTC GGCAGCCTGT GGTGATTCTC 
TTGGGCTGGG GTGGCTGCAA GGACAAGAAC CTTGCCAAGT ACAGTGCCAT CTACCACAAA 
AGGGGCTGCA TCGTAATCCG ATACACAGCC CCGTGGCACA TGGTCTTCTT CTCCGAGTCA 
CTGGGTATCC CTTCACTTCG TGTTTTGGCC CAGAAGCTGC TCGAGCTGCT CTTTGATTAT 
GAGATTGAGA AGGAGCCCCT GCTCTTCCAT GTCTTCAGCA ACGGTGGCGT CATGCTGTAC 
CGCTACGTGC TGGAGCTCCT GCAGACCCGT CGCTTCTGCC GCCTGCGTGT GGTGGGCACC 
ATCTTTGACA GCGCTCCTGG TGACAGCAAC CTGGTAGGGG CTCTGCGGGC CCTGGCAGCC 
ATCCTGGAGC GCCGGGCCGC CATGCTGCGC CTGTTGCTGC TGGTGGCCTT TGCCCTGGTG 
GTCGTCCTGT TCCACGTCCT GCTTGCTCCC ATCACAGCCC TCTTCCACAC CCACTTCTAT 
GACAGGCTAC AGGACGCGGG CTCTCGCTGG CCCGAGCTCT ACCTCTACTC GAGGGCTGAC 
GAAGTAGTCC TGGCCAGAGA CATAGAACGC ATGGTGGAGG CACGCCTGGC ACGCCGGGTC 
CTGGCGCGTT CTGTGGATTT CGTGTCATCT GCACACGTCA GCCACCTCCG TGACTACCCT 
ACTTACTACA CAAGCCTCTG TGTCGACTTC ATGCGCAACT GCGTCCGCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_078863.2
CDS	218..1051
Translation	MASAELDYTIEIPDQPCWSQKNSPSPGGKEAETRQPVVILLGWGGCKDKNLAKYSAIYHKRGCIVIRYTAPWHMVFFSESLGIPSLRVLAQKLLELLFDYEIEKEPLLFHVFSNGGVMLYRYVLELLQTRRFCRLRVVGTIFDSAPGDSNLVGALRALAAILERRAAMLRLLLLVAFALVVVLFHVLLAPITALFHTHFYDRLQDAGSRWPELYLYSRADEVVLARDIERMVEARLARRVLARSVDFVSSAHVSHLRDYPTYYTSLCVDFMRNCVRC

Target ORF information:

RefSeq Version	NM_024587.3
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 53 (TMEM53), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024587.3

ORF Insert Sequence:

ATGGCCTCGG CAGAGCTGGA CTACACCATC GAGATCCCGG ATCAGCCCTG CTGGAGCCAG 
AAGAACAGCC CCAGCCCAGG TGGGAAGGAG GCAGAAACTC GGCAGCCTGT GGTGATTCTC 
TTGGGCTGGG GTGGCTGCAA GGACAAGAAC CTTGCCAAGT ACAGTGCCAT CTACCACAAA 
AGGGGCTGCA TCGTAATCCG ATACACAGCC CCGTGGCACA TGGTCTTCTT CTCCGAGTCA 
CTGGGTATCC CTTCACTTCG TGTTTTGGCC CAGAAGCTGC TCGAGCTGCT CTTTGATTAT 
GAGATTGAGA AGGAGCCCCT GCTCTTCCAT GTCTTCAGCA ACGGTGGCGT CATGCTGTAC 
CGCTACGTGC TGGAGCTCCT GCAGACCCGT CGCTTCTGCC GCCTGCGTGT GGTGGGCACC 
ATCTTTGACA GCGCTCCTGG TGACAGCAAC CTGGTAGGGG CTCTGCGGGC CCTGGCAGCC 
ATCCTGGAGC GCCGGGCCGC CATGCTGCGC CTGTTGCTGC TGGTGGCCTT TGCCCTGGTG 
GTCGTCCTGT TCCACGTCCT GCTTGCTCCC ATCACAGCCC TCTTCCACAC CCACTTCTAT 
GACAGGCTAC AGGACGCGGG CTCTCGCTGG CCCGAGCTCT ACCTCTACTC GAGGGCTGAC 
GAAGTAGTCC TGGCCAGAGA CATAGAACGC ATGGTGGAGG CACGCCTGGC ACGCCGGGTC 
CTGGCGCGTT CTGTGGATTT CGTGTCATCT GCACACGTCA GCCACCTCCG TGACTACCCT 
ACTTACTACA CAAGCCTCTG TGTCGACTTC ATGCGCAACT GCGTCCGCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM53 ( NM_024587.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_078863.2 Homo sapiens transmembrane protein 53 (TMEM53), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu13124
Clone ID Related Accession (Same CDS sequence)	NM_024587.3 , NM_024587.4
Accession Version	NM_024587.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Homo sapiens(human)
Product	transmembrane protein 53 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY108001.1, BC064520.1 and BM675039.1. On Nov 22, 2018 this sequence version replaced NM_024587.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1803617.147921.1, SRR1660809.150841.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153307, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000372237.8/ ENSP00000361311.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCTCGG CAGAGCTGGA CTACACCATC GAGATCCCGG ATCAGCCCTG CTGGAGCCAG 
AAGAACAGCC CCAGCCCAGG TGGGAAGGAG GCAGAAACTC GGCAGCCTGT GGTGATTCTC 
TTGGGCTGGG GTGGCTGCAA GGACAAGAAC CTTGCCAAGT ACAGTGCCAT CTACCACAAA 
AGGGGCTGCA TCGTAATCCG ATACACAGCC CCGTGGCACA TGGTCTTCTT CTCCGAGTCA 
CTGGGTATCC CTTCACTTCG TGTTTTGGCC CAGAAGCTGC TCGAGCTGCT CTTTGATTAT 
GAGATTGAGA AGGAGCCCCT GCTCTTCCAT GTCTTCAGCA ACGGTGGCGT CATGCTGTAC 
CGCTACGTGC TGGAGCTCCT GCAGACCCGT CGCTTCTGCC GCCTGCGTGT GGTGGGCACC 
ATCTTTGACA GCGCTCCTGG TGACAGCAAC CTGGTAGGGG CTCTGCGGGC CCTGGCAGCC 
ATCCTGGAGC GCCGGGCCGC CATGCTGCGC CTGTTGCTGC TGGTGGCCTT TGCCCTGGTG 
GTCGTCCTGT TCCACGTCCT GCTTGCTCCC ATCACAGCCC TCTTCCACAC CCACTTCTAT 
GACAGGCTAC AGGACGCGGG CTCTCGCTGG CCCGAGCTCT ACCTCTACTC GAGGGCTGAC 
GAAGTAGTCC TGGCCAGAGA CATAGAACGC ATGGTGGAGG CACGCCTGGC ACGCCGGGTC 
CTGGCGCGTT CTGTGGATTT CGTGTCATCT GCACACGTCA GCCACCTCCG TGACTACCCT 
ACTTACTACA CAAGCCTCTG TGTCGACTTC ATGCGCAACT GCGTCCGCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_078863.2
CDS	91..924
Translation	MASAELDYTIEIPDQPCWSQKNSPSPGGKEAETRQPVVILLGWGGCKDKNLAKYSAIYHKRGCIVIRYTAPWHMVFFSESLGIPSLRVLAQKLLELLFDYEIEKEPLLFHVFSNGGVMLYRYVLELLQTRRFCRLRVVGTIFDSAPGDSNLVGALRALAAILERRAAMLRLLLLVAFALVVVLFHVLLAPITALFHTHFYDRLQDAGSRWPELYLYSRADEVVLARDIERMVEARLARRVLARSVDFVSSAHVSHLRDYPTYYTSLCVDFMRNCVRC

Target ORF information:

RefSeq Version	NM_024587.4
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 53 (TMEM53), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024587.4

ORF Insert Sequence:

ATGGCCTCGG CAGAGCTGGA CTACACCATC GAGATCCCGG ATCAGCCCTG CTGGAGCCAG 
AAGAACAGCC CCAGCCCAGG TGGGAAGGAG GCAGAAACTC GGCAGCCTGT GGTGATTCTC 
TTGGGCTGGG GTGGCTGCAA GGACAAGAAC CTTGCCAAGT ACAGTGCCAT CTACCACAAA 
AGGGGCTGCA TCGTAATCCG ATACACAGCC CCGTGGCACA TGGTCTTCTT CTCCGAGTCA 
CTGGGTATCC CTTCACTTCG TGTTTTGGCC CAGAAGCTGC TCGAGCTGCT CTTTGATTAT 
GAGATTGAGA AGGAGCCCCT GCTCTTCCAT GTCTTCAGCA ACGGTGGCGT CATGCTGTAC 
CGCTACGTGC TGGAGCTCCT GCAGACCCGT CGCTTCTGCC GCCTGCGTGT GGTGGGCACC 
ATCTTTGACA GCGCTCCTGG TGACAGCAAC CTGGTAGGGG CTCTGCGGGC CCTGGCAGCC 
ATCCTGGAGC GCCGGGCCGC CATGCTGCGC CTGTTGCTGC TGGTGGCCTT TGCCCTGGTG 
GTCGTCCTGT TCCACGTCCT GCTTGCTCCC ATCACAGCCC TCTTCCACAC CCACTTCTAT 
GACAGGCTAC AGGACGCGGG CTCTCGCTGG CCCGAGCTCT ACCTCTACTC GAGGGCTGAC 
GAAGTAGTCC TGGCCAGAGA CATAGAACGC ATGGTGGAGG CACGCCTGGC ACGCCGGGTC 
CTGGCGCGTT CTGTGGATTT CGTGTCATCT GCACACGTCA GCCACCTCCG TGACTACCCT 
ACTTACTACA CAAGCCTCTG TGTCGACTTC ATGCGCAACT GCGTCCGCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM53 ( XM_011542138.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011540440.1 Homo sapiens transmembrane protein 53 (TMEM53), transcript variant X1, mRNA.

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CloneID	OHu71957
Clone ID Related Accession (Same CDS sequence)	XM_011542138.2
Accession Version	XM_011542138.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	transmembrane protein 53 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011542138.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTACC GCTACGTGCT GGAGCTCCTG CAGACCCGTC GCTTCTGCCG CCTGCGTGTG 
GTGGGCACCA TCTTTGACAG CGCTCCTGGT GACAGCAACC TGGTAGGGGC TCTGCGGGCC 
CTGGCAGCCA TCCTGGAGCG CCGGGCCGCC ATGCTGCGCC TGTTGCTGCT GGTGGCCTTT 
GCCCTGGTGG TCGTCCTGTT CCACGTCCTG CTTGCTCCCA TCACAGCCCT CTTCCACACC 
CACTTCTATG ACAGGCTACA GGACGCGGGC TCTCGCTGGC CCGAGCTCTA CCTCTACTCG 
AGGGCTGACG AAGTAGTCCT GGCCAGAGAC ATAGAACGCA TGGTGGAGGC ACGCCTGGCA 
CGCCGGGTCC TGGCGCGTTC TGTGGATTTC GTGTCATCTG CACACGTCAG CCACCTCCGT 
GACTACCCTA CTTACTACAC AAGCCTCTGT GTCGACTTCA TGCGCAACTG CGTCCGCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011540440.1
CDS	698..1180
Translation	MLYRYVLELLQTRRFCRLRVVGTIFDSAPGDSNLVGALRALAAILERRAAMLRLLLLVAFALVVVLFHVLLAPITALFHTHFYDRLQDAGSRWPELYLYSRADEVVLARDIERMVEARLARRVLARSVDFVSSAHVSHLRDYPTYYTSLCVDFMRNCVRC

Target ORF information:

RefSeq Version	XM_011542138.2
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 53 (TMEM53), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011542138.2

ORF Insert Sequence:

ATGCTGTACC GCTACGTGCT GGAGCTCCTG CAGACCCGTC GCTTCTGCCG CCTGCGTGTG 
GTGGGCACCA TCTTTGACAG CGCTCCTGGT GACAGCAACC TGGTAGGGGC TCTGCGGGCC 
CTGGCAGCCA TCCTGGAGCG CCGGGCCGCC ATGCTGCGCC TGTTGCTGCT GGTGGCCTTT 
GCCCTGGTGG TCGTCCTGTT CCACGTCCTG CTTGCTCCCA TCACAGCCCT CTTCCACACC 
CACTTCTATG ACAGGCTACA GGACGCGGGC TCTCGCTGGC CCGAGCTCTA CCTCTACTCG 
AGGGCTGACG AAGTAGTCCT GGCCAGAGAC ATAGAACGCA TGGTGGAGGC ACGCCTGGCA 
CGCCGGGTCC TGGCGCGTTC TGTGGATTTC GTGTCATCTG CACACGTCAG CCACCTCCGT 
GACTACCCTA CTTACTACAC AAGCCTCTGT GTCGACTTCA TGCGCAACTG CGTCCGCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM53 ( NM_001300747.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001287676.1 Homo sapiens transmembrane protein 53 (TMEM53), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu33798
Clone ID Related Accession (Same CDS sequence)	NM_001300747.1 , NM_001300747.2
Accession Version	NM_001300747.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-03
Organism	Homo sapiens(human)
Product	transmembrane protein 53 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY108001.1, HY029536.1, BC064520.1 and BM675039.1. On Jul 11, 2014 this sequence version replaced XM_005271194.2. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.246850.1, SRR1660805.350938.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2153307, SAMEA2154665 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTCGG CAGAGCTGGA CTACACCATC GAGATCCCGG ATCAGCCCTG CTGGAGCCAG 
AAGAACAGCC CCAGCCCAGG TGGGAAGGAG GCAGAAACTC GGCAGCCTGT GGTGATTCTC 
TTGGGCTGGG GTGGCTGCAA GGACAAGAAC CTTGCCAAGT ACAGTGCCAT CTACCACAAA 
AGGAAGCTGC TCGAGCTGCT CTTTGATTAT GAGATTGAGA AGGAGCCCCT GCTCTTCCAT 
GTCTTCAGCA ACGGTGGCGT CATGCTGTAC CGCTACGTGC TGGAGCTCCT GCAGACCCGT 
CGCTTCTGCC GCCTGCGTGT GGTGGGCACC ATCTTTGACA GCGCTCCTGG TGACAGCAAC 
CTGGTAGGGG CTCTGCGGGC CCTGGCAGCC ATCCTGGAGC GCCGGGCCGC CATGCTGCGC 
CTGTTGCTGC TGGTGGCCTT TGCCCTGGTG GTCGTCCTGT TCCACGTCCT GCTTGCTCCC 
ATCACAGCCC TCTTCCACAC CCACTTCTAT GACAGGCTAC AGGACGCGGG CTCTCGCTGG 
CCCGAGCTCT ACCTCTACTC GAGGGCTGAC GAAGTAGTCC TGGCCAGAGA CATAGAACGC 
ATGGTGGAGG CACGCCTGGC ACGCCGGGTC CTGGCGCGTT CTGTGGATTT CGTGTCATCT 
GCACACGTCA GCCACCTCCG TGACTACCCT ACTTACTACA CAAGCCTCTG TGTCGACTTC 
ATGCGCAACT GCGTCCGCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287676.1
CDS	218..961
Translation	MASAELDYTIEIPDQPCWSQKNSPSPGGKEAETRQPVVILLGWGGCKDKNLAKYSAIYHKRKLLELLFDYEIEKEPLLFHVFSNGGVMLYRYVLELLQTRRFCRLRVVGTIFDSAPGDSNLVGALRALAAILERRAAMLRLLLLVAFALVVVLFHVLLAPITALFHTHFYDRLQDAGSRWPELYLYSRADEVVLARDIERMVEARLARRVLARSVDFVSSAHVSHLRDYPTYYTSLCVDFMRNCVRC

Target ORF information:

RefSeq Version	NM_001300747.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 53 (TMEM53), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300747.1

ORF Insert Sequence:

ATGGCCTCGG CAGAGCTGGA CTACACCATC GAGATCCCGG ATCAGCCCTG CTGGAGCCAG 
AAGAACAGCC CCAGCCCAGG TGGGAAGGAG GCAGAAACTC GGCAGCCTGT GGTGATTCTC 
TTGGGCTGGG GTGGCTGCAA GGACAAGAAC CTTGCCAAGT ACAGTGCCAT CTACCACAAA 
AGGAAGCTGC TCGAGCTGCT CTTTGATTAT GAGATTGAGA AGGAGCCCCT GCTCTTCCAT 
GTCTTCAGCA ACGGTGGCGT CATGCTGTAC CGCTACGTGC TGGAGCTCCT GCAGACCCGT 
CGCTTCTGCC GCCTGCGTGT GGTGGGCACC ATCTTTGACA GCGCTCCTGG TGACAGCAAC 
CTGGTAGGGG CTCTGCGGGC CCTGGCAGCC ATCCTGGAGC GCCGGGCCGC CATGCTGCGC 
CTGTTGCTGC TGGTGGCCTT TGCCCTGGTG GTCGTCCTGT TCCACGTCCT GCTTGCTCCC 
ATCACAGCCC TCTTCCACAC CCACTTCTAT GACAGGCTAC AGGACGCGGG CTCTCGCTGG 
CCCGAGCTCT ACCTCTACTC GAGGGCTGAC GAAGTAGTCC TGGCCAGAGA CATAGAACGC 
ATGGTGGAGG CACGCCTGGC ACGCCGGGTC CTGGCGCGTT CTGTGGATTT CGTGTCATCT 
GCACACGTCA GCCACCTCCG TGACTACCCT ACTTACTACA CAAGCCTCTG TGTCGACTTC 
ATGCGCAACT GCGTCCGCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM53 ( NM_001300747.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001287676.1 Homo sapiens transmembrane protein 53 (TMEM53), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu33798
Clone ID Related Accession (Same CDS sequence)	NM_001300747.1 , NM_001300747.2
Accession Version	NM_001300747.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-31
Organism	Homo sapiens(human)
Product	transmembrane protein 53 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY108001.1, HY029536.1, BC064520.1 and BM675039.1. On Jun 1, 2019 this sequence version replaced NM_001300747.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.246850.1, SRR1660805.350938.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2153307, SAMEA2154665 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTCGG CAGAGCTGGA CTACACCATC GAGATCCCGG ATCAGCCCTG CTGGAGCCAG 
AAGAACAGCC CCAGCCCAGG TGGGAAGGAG GCAGAAACTC GGCAGCCTGT GGTGATTCTC 
TTGGGCTGGG GTGGCTGCAA GGACAAGAAC CTTGCCAAGT ACAGTGCCAT CTACCACAAA 
AGGAAGCTGC TCGAGCTGCT CTTTGATTAT GAGATTGAGA AGGAGCCCCT GCTCTTCCAT 
GTCTTCAGCA ACGGTGGCGT CATGCTGTAC CGCTACGTGC TGGAGCTCCT GCAGACCCGT 
CGCTTCTGCC GCCTGCGTGT GGTGGGCACC ATCTTTGACA GCGCTCCTGG TGACAGCAAC 
CTGGTAGGGG CTCTGCGGGC CCTGGCAGCC ATCCTGGAGC GCCGGGCCGC CATGCTGCGC 
CTGTTGCTGC TGGTGGCCTT TGCCCTGGTG GTCGTCCTGT TCCACGTCCT GCTTGCTCCC 
ATCACAGCCC TCTTCCACAC CCACTTCTAT GACAGGCTAC AGGACGCGGG CTCTCGCTGG 
CCCGAGCTCT ACCTCTACTC GAGGGCTGAC GAAGTAGTCC TGGCCAGAGA CATAGAACGC 
ATGGTGGAGG CACGCCTGGC ACGCCGGGTC CTGGCGCGTT CTGTGGATTT CGTGTCATCT 
GCACACGTCA GCCACCTCCG TGACTACCCT ACTTACTACA CAAGCCTCTG TGTCGACTTC 
ATGCGCAACT GCGTCCGCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287676.1
CDS	91..834
Translation	MASAELDYTIEIPDQPCWSQKNSPSPGGKEAETRQPVVILLGWGGCKDKNLAKYSAIYHKRKLLELLFDYEIEKEPLLFHVFSNGGVMLYRYVLELLQTRRFCRLRVVGTIFDSAPGDSNLVGALRALAAILERRAAMLRLLLLVAFALVVVLFHVLLAPITALFHTHFYDRLQDAGSRWPELYLYSRADEVVLARDIERMVEARLARRVLARSVDFVSSAHVSHLRDYPTYYTSLCVDFMRNCVRC

Target ORF information:

RefSeq Version	NM_001300747.2
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 53 (TMEM53), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300747.2

ORF Insert Sequence:

ATGGCCTCGG CAGAGCTGGA CTACACCATC GAGATCCCGG ATCAGCCCTG CTGGAGCCAG 
AAGAACAGCC CCAGCCCAGG TGGGAAGGAG GCAGAAACTC GGCAGCCTGT GGTGATTCTC 
TTGGGCTGGG GTGGCTGCAA GGACAAGAAC CTTGCCAAGT ACAGTGCCAT CTACCACAAA 
AGGAAGCTGC TCGAGCTGCT CTTTGATTAT GAGATTGAGA AGGAGCCCCT GCTCTTCCAT 
GTCTTCAGCA ACGGTGGCGT CATGCTGTAC CGCTACGTGC TGGAGCTCCT GCAGACCCGT 
CGCTTCTGCC GCCTGCGTGT GGTGGGCACC ATCTTTGACA GCGCTCCTGG TGACAGCAAC 
CTGGTAGGGG CTCTGCGGGC CCTGGCAGCC ATCCTGGAGC GCCGGGCCGC CATGCTGCGC 
CTGTTGCTGC TGGTGGCCTT TGCCCTGGTG GTCGTCCTGT TCCACGTCCT GCTTGCTCCC 
ATCACAGCCC TCTTCCACAC CCACTTCTAT GACAGGCTAC AGGACGCGGG CTCTCGCTGG 
CCCGAGCTCT ACCTCTACTC GAGGGCTGAC GAAGTAGTCC TGGCCAGAGA CATAGAACGC 
ATGGTGGAGG CACGCCTGGC ACGCCGGGTC CTGGCGCGTT CTGTGGATTT CGTGTCATCT 
GCACACGTCA GCCACCTCCG TGACTACCCT ACTTACTACA CAAGCCTCTG TGTCGACTTC 
ATGCGCAACT GCGTCCGCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM53 ( NM_001300748.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001287677.1 Homo sapiens transmembrane protein 53 (TMEM53), transcript variant 4, mRNA.

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CloneID	OHu33799
Clone ID Related Accession (Same CDS sequence)	NM_001300748.2 , NM_001300748.1
Accession Version	NM_001300748.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	transmembrane protein 53 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY108001.1, AA490195.1, BC064520.1 and BM675039.1. On May 31, 2019 this sequence version replaced NM_001300748.1. Transcript Variant: This variant (4) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (4) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.355435.1, SRR1163657.399564.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153307, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTCGG CAGAGCTGGA CTACACCATC GAGATCCCGG ATCAGCCCTG CTGGAGCCAG 
AAGAACAGCC CCAGCCCAGG TGGGAAGGAG GCAGAAACTC GGCAGCCTGT GGTGATTCTC 
TTGGGCTGGG GTGGCTGCAA GGACAAGAAC CTTGCCAAGT ACAGTGCCAT CTACCACAAA 
AGGCTGCTCG AGCTGCTCTT TGATTATGAG ATTGAGAAGG AGCCCCTGCT CTTCCATGTC 
TTCAGCAACG GTGGCGTCAT GCTGTACCGC TACGTGCTGG AGCTCCTGCA GACCCGTCGC 
TTCTGCCGCC TGCGTGTGGT GGGCACCATC TTTGACAGCG CTCCTGGTGA CAGCAACCTG 
GTAGGGGCTC TGCGGGCCCT GGCAGCCATC CTGGAGCGCC GGGCCGCCAT GCTGCGCCTG 
TTGCTGCTGG TGGCCTTTGC CCTGGTGGTC GTCCTGTTCC ACGTCCTGCT TGCTCCCATC 
ACAGCCCTCT TCCACACCCA CTTCTATGAC AGGCTACAGG ACGCGGGCTC TCGCTGGCCC 
GAGCTCTACC TCTACTCGAG GGCTGACGAA GTAGTCCTGG CCAGAGACAT AGAACGCATG 
GTGGAGGCAC GCCTGGCACG CCGGGTCCTG GCGCGTTCTG TGGATTTCGT GTCATCTGCA 
CACGTCAGCC ACCTCCGTGA CTACCCTACT TACTACACAA GCCTCTGTGT CGACTTCATG 
CGCAACTGCG TCCGCTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287677.1
CDS	91..831
Translation	MASAELDYTIEIPDQPCWSQKNSPSPGGKEAETRQPVVILLGWGGCKDKNLAKYSAIYHKRLLELLFDYEIEKEPLLFHVFSNGGVMLYRYVLELLQTRRFCRLRVVGTIFDSAPGDSNLVGALRALAAILERRAAMLRLLLLVAFALVVVLFHVLLAPITALFHTHFYDRLQDAGSRWPELYLYSRADEVVLARDIERMVEARLARRVLARSVDFVSSAHVSHLRDYPTYYTSLCVDFMRNCVRC

Target ORF information:

RefSeq Version	NM_001300748.2
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 53 (TMEM53), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300748.2

ORF Insert Sequence:

ATGGCCTCGG CAGAGCTGGA CTACACCATC GAGATCCCGG ATCAGCCCTG CTGGAGCCAG 
AAGAACAGCC CCAGCCCAGG TGGGAAGGAG GCAGAAACTC GGCAGCCTGT GGTGATTCTC 
TTGGGCTGGG GTGGCTGCAA GGACAAGAAC CTTGCCAAGT ACAGTGCCAT CTACCACAAA 
AGGCTGCTCG AGCTGCTCTT TGATTATGAG ATTGAGAAGG AGCCCCTGCT CTTCCATGTC 
TTCAGCAACG GTGGCGTCAT GCTGTACCGC TACGTGCTGG AGCTCCTGCA GACCCGTCGC 
TTCTGCCGCC TGCGTGTGGT GGGCACCATC TTTGACAGCG CTCCTGGTGA CAGCAACCTG 
GTAGGGGCTC TGCGGGCCCT GGCAGCCATC CTGGAGCGCC GGGCCGCCAT GCTGCGCCTG 
TTGCTGCTGG TGGCCTTTGC CCTGGTGGTC GTCCTGTTCC ACGTCCTGCT TGCTCCCATC 
ACAGCCCTCT TCCACACCCA CTTCTATGAC AGGCTACAGG ACGCGGGCTC TCGCTGGCCC 
GAGCTCTACC TCTACTCGAG GGCTGACGAA GTAGTCCTGG CCAGAGACAT AGAACGCATG 
GTGGAGGCAC GCCTGGCACG CCGGGTCCTG GCGCGTTCTG TGGATTTCGT GTCATCTGCA 
CACGTCAGCC ACCTCCGTGA CTACCCTACT TACTACACAA GCCTCTGTGT CGACTTCATG 
CGCAACTGCG TCCGCTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM53 ( NM_001300748.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001287677.1 Homo sapiens transmembrane protein 53 (TMEM53), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu33799
Clone ID Related Accession (Same CDS sequence)	NM_001300748.2 , NM_001300748.1
Accession Version	NM_001300748.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-03
Organism	Homo sapiens(human)
Product	transmembrane protein 53 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY108001.1, AA490195.1, BC064520.1 and BM675039.1. On Jul 11, 2014 this sequence version replaced XM_005271195.2. Transcript Variant: This variant (4) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (4) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.355435.1, SRR1163657.399564.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153307, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTCGG CAGAGCTGGA CTACACCATC GAGATCCCGG ATCAGCCCTG CTGGAGCCAG 
AAGAACAGCC CCAGCCCAGG TGGGAAGGAG GCAGAAACTC GGCAGCCTGT GGTGATTCTC 
TTGGGCTGGG GTGGCTGCAA GGACAAGAAC CTTGCCAAGT ACAGTGCCAT CTACCACAAA 
AGGCTGCTCG AGCTGCTCTT TGATTATGAG ATTGAGAAGG AGCCCCTGCT CTTCCATGTC 
TTCAGCAACG GTGGCGTCAT GCTGTACCGC TACGTGCTGG AGCTCCTGCA GACCCGTCGC 
TTCTGCCGCC TGCGTGTGGT GGGCACCATC TTTGACAGCG CTCCTGGTGA CAGCAACCTG 
GTAGGGGCTC TGCGGGCCCT GGCAGCCATC CTGGAGCGCC GGGCCGCCAT GCTGCGCCTG 
TTGCTGCTGG TGGCCTTTGC CCTGGTGGTC GTCCTGTTCC ACGTCCTGCT TGCTCCCATC 
ACAGCCCTCT TCCACACCCA CTTCTATGAC AGGCTACAGG ACGCGGGCTC TCGCTGGCCC 
GAGCTCTACC TCTACTCGAG GGCTGACGAA GTAGTCCTGG CCAGAGACAT AGAACGCATG 
GTGGAGGCAC GCCTGGCACG CCGGGTCCTG GCGCGTTCTG TGGATTTCGT GTCATCTGCA 
CACGTCAGCC ACCTCCGTGA CTACCCTACT TACTACACAA GCCTCTGTGT CGACTTCATG 
CGCAACTGCG TCCGCTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287677.1
CDS	218..958
Translation	MASAELDYTIEIPDQPCWSQKNSPSPGGKEAETRQPVVILLGWGGCKDKNLAKYSAIYHKRLLELLFDYEIEKEPLLFHVFSNGGVMLYRYVLELLQTRRFCRLRVVGTIFDSAPGDSNLVGALRALAAILERRAAMLRLLLLVAFALVVVLFHVLLAPITALFHTHFYDRLQDAGSRWPELYLYSRADEVVLARDIERMVEARLARRVLARSVDFVSSAHVSHLRDYPTYYTSLCVDFMRNCVRC

Target ORF information:

RefSeq Version	NM_001300748.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 53 (TMEM53), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300748.1

ORF Insert Sequence:

ATGGCCTCGG CAGAGCTGGA CTACACCATC GAGATCCCGG ATCAGCCCTG CTGGAGCCAG 
AAGAACAGCC CCAGCCCAGG TGGGAAGGAG GCAGAAACTC GGCAGCCTGT GGTGATTCTC 
TTGGGCTGGG GTGGCTGCAA GGACAAGAAC CTTGCCAAGT ACAGTGCCAT CTACCACAAA 
AGGCTGCTCG AGCTGCTCTT TGATTATGAG ATTGAGAAGG AGCCCCTGCT CTTCCATGTC 
TTCAGCAACG GTGGCGTCAT GCTGTACCGC TACGTGCTGG AGCTCCTGCA GACCCGTCGC 
TTCTGCCGCC TGCGTGTGGT GGGCACCATC TTTGACAGCG CTCCTGGTGA CAGCAACCTG 
GTAGGGGCTC TGCGGGCCCT GGCAGCCATC CTGGAGCGCC GGGCCGCCAT GCTGCGCCTG 
TTGCTGCTGG TGGCCTTTGC CCTGGTGGTC GTCCTGTTCC ACGTCCTGCT TGCTCCCATC 
ACAGCCCTCT TCCACACCCA CTTCTATGAC AGGCTACAGG ACGCGGGCTC TCGCTGGCCC 
GAGCTCTACC TCTACTCGAG GGCTGACGAA GTAGTCCTGG CCAGAGACAT AGAACGCATG 
GTGGAGGCAC GCCTGGCACG CCGGGTCCTG GCGCGTTCTG TGGATTTCGT GTCATCTGCA 
CACGTCAGCC ACCTCCGTGA CTACCCTACT TACTACACAA GCCTCTGTGT CGACTTCATG 
CGCAACTGCG TCCGCTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM53 ( NM_001300746.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001287675.1 Homo sapiens transmembrane protein 53 (TMEM53), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu33800
Clone ID Related Accession (Same CDS sequence)	NM_001300746.1
Accession Version	NM_001300746.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	transmembrane protein 53 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY108001.1, AL832539.1 and BM675039.1. On Jul 11, 2014 this sequence version replaced XM_005271196.2. Transcript Variant: This variant (2) lacks an alternate coding exon and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.277949.1, AL832539.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTCTTCT TCTCCGAGTC ACTGGGTATC CCTTCACTTC GTGTTTTGGC CCAGAAGCTG 
CTCGAGCTGC TCTTTGATTA TGAGATTGAG AAGGAGCCCC TGCTCTTCCA TGTCTTCAGC 
AACGGTGGCG TCATGCTGTA CCGCTACGTG CTGGAGCTCC TGCAGACCCG TCGCTTCTGC 
CGCCTGCGTG TGGTGGGCAC CATCTTTGAC AGCGCTCCTG GTGACAGCAA CCTGGTAGGG 
GCTCTGCGGG CCCTGGCAGC CATCCTGGAG CGCCGGGCCG CCATGCTGCG CCTGTTGCTG 
CTGGTGGCCT TTGCCCTGGT GGTCGTCCTG TTCCACGTCC TGCTTGCTCC CATCACAGCC 
CTCTTCCACA CCCACTTCTA TGACAGGCTA CAGGACGCGG GCTCTCGCTG GCCCGAGCTC 
TACCTCTACT CGAGGGCTGA CGAAGTAGTC CTGGCCAGAG ACATAGAACG CATGGTGGAG 
GCACGCCTGG CACGCCGGGT CCTGGCGCGT TCTGTGGATT TCGTGTCATC TGCACACGTC 
AGCCACCTCC GTGACTACCC TACTTACTAC ACAAGCCTCT GTGTCGACTT CATGCGCAAC 
TGCGTCCGCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287675.1
CDS	315..929
Translation	MVFFSESLGIPSLRVLAQKLLELLFDYEIEKEPLLFHVFSNGGVMLYRYVLELLQTRRFCRLRVVGTIFDSAPGDSNLVGALRALAAILERRAAMLRLLLLVAFALVVVLFHVLLAPITALFHTHFYDRLQDAGSRWPELYLYSRADEVVLARDIERMVEARLARRVLARSVDFVSSAHVSHLRDYPTYYTSLCVDFMRNCVRC

Target ORF information:

RefSeq Version	NM_001300746.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 53 (TMEM53), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300746.1

ORF Insert Sequence:

ATGGTCTTCT TCTCCGAGTC ACTGGGTATC CCTTCACTTC GTGTTTTGGC CCAGAAGCTG 
CTCGAGCTGC TCTTTGATTA TGAGATTGAG AAGGAGCCCC TGCTCTTCCA TGTCTTCAGC 
AACGGTGGCG TCATGCTGTA CCGCTACGTG CTGGAGCTCC TGCAGACCCG TCGCTTCTGC 
CGCCTGCGTG TGGTGGGCAC CATCTTTGAC AGCGCTCCTG GTGACAGCAA CCTGGTAGGG 
GCTCTGCGGG CCCTGGCAGC CATCCTGGAG CGCCGGGCCG CCATGCTGCG CCTGTTGCTG 
CTGGTGGCCT TTGCCCTGGT GGTCGTCCTG TTCCACGTCC TGCTTGCTCC CATCACAGCC 
CTCTTCCACA CCCACTTCTA TGACAGGCTA CAGGACGCGG GCTCTCGCTG GCCCGAGCTC 
TACCTCTACT CGAGGGCTGA CGAAGTAGTC CTGGCCAGAG ACATAGAACG CATGGTGGAG 
GCACGCCTGG CACGCCGGGT CCTGGCGCGT TCTGTGGATT TCGTGTCATC TGCACACGTC 
AGCCACCTCC GTGACTACCC TACTTACTAC ACAAGCCTCT GTGTCGACTT CATGCGCAAC 
TGCGTCCGCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Proteomic characterization of the human sperm nucleus.
Proteomics11(13)2714-26(2011 Jul)
de Mateo S,Castillo J,Estanyol JM,Ballesc? JL,Oliva R

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TMEM53 cDNA ORF clone, Homo sapiens(human)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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