LOC112277155 cDNA ORF clone,

Gene
Clones
gRNAs

The following LOC112277155 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC112277155 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPn65283	XM_024509187.1 Latest version!	Physcomitrella patens probable leucine-rich repeat receptor-like protein kinase At1g35710 (LOC112277155), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OPn65284	XM_024509188.1 Latest version!	Physcomitrella patens probable leucine-rich repeat receptor-like protein kinase At1g35710 (LOC112277155), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC112277155 ( XM_024509187.1 ) cDNA ORF clone, -> XP_024364955.1 Physcomitrella patens probable leucine-rich repeat receptor-like protein kinase At1g35710 (LOC112277155), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPn65283
Clone ID Related Accession (Same CDS sequence)	XM_024509187.1
Accession Version	XM_024509187.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Physcomitrella patens
Product	probable LRR receptor-like serine/threonine-protein kinase At1g34110 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037277.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physcomitrella patens Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA CAATCCTTCT CCTGCTGGCA GTTTGCTTCT TGCTTACGGC GTCATATTTG 
ACGGCTGAGA GCTTTAGGGA CGAGGACTGG GACGATGAGT CGGAAGAGTC CACAGATTTT 
ACCAAGGTTC GCCAAGAAGA ATCTGAGAGA TTCAAGAGAC TCGTCTCAAT ACCCAATGAA 
TTCGACCCAT CGCTCTTTGA AGGCTTGATC CATGACGATT TTGAAGAAGA CGCCCTGCAC 
GAGCACCCTG GGGGGCGACA GTTACTTCAA GCTGCCAACA TTCTCGCTAG CGAACAGGAT 
GCGCTCTTGC AGTTTAAGAG AGGAGTACTC GATCCTAATG GTATTCTGGC AGGATGGAAT 
GGAACTTCGA ATCAAGACCA CTGCGAATGG GTAGGCATTC TTTGCAGCAA CATCACGAGT 
AATAACACCA TTCGTCGAGT AGTGCAATTA AATATCACAG GTGTCGAAGG TGCTAAGCTG 
GGAGGTTTGA TTTCCCCGAA CATCTCACTG CTGTCTTCGC TGACAGTCCT AATCCTGCAA 
AGTAACCTAT TAACGGGACC TATTCCCCCA GAGTTGGGGA ATTTGAAAAA TTTGAAGACC 
CTGAATTTAC ATGGCAACAA TCTGACGTCA TACATCCCGG TTCAGCTTTC GAACCTTACG 
CTCCTCCAGA CTCTGGATAT TGGCTCCAAT AATATGACGG GTGGATTGCC AAAAGAGTTA 
GCACAATGTA AGAATATGCT CCAATTGGAT GTGTCTTCAA ATCATTTCGA CGGAGAAATT 
CACAGCGACT TCGGTACTTT TCCAAAGTTG AAAATGTTTT TGGCCATGAA CAACGAGTTA 
ACCGGAAGTA TCCCTGAGAG TTTTGGAAAT TGTACCACTC TGGAGAGCTT TGCGGTGAAT 
GACAATCATT TGGTAGGAGA GATTCCGAGA GGCTTTGCAA ATGCTCCCAA TTTGCAAGGA 
TTTCTGGTGG GGTTTAACAA CATCACCGGA ACGCTTCCTG AGGGGTTTGG GAAGTTGCAG 
AAGTTGAGTA TTGTCTACTT CCAGTATAAC AAGATGAACG GAACCATGGA TTTTCTGCAG 
AATTGCAGCG GAATGTGGCA AATTCATGGC GAGAATAACA ACCTCACTGG GCGACTTCCT 
ACTTTCTTTG GGGATACATG CGAGAACCTG ACGCATGTCT TTGTATCGAG AAACAACTTC 
TCCGGACCAC TTCCGGGTAC CCTATCGAAG TGCCCCAAGT TGGAGAATAT CGGCGTTAGC 
AACAACAATC TCTCCGGCAC CATCCCGCCA GAATTTAGCA TTTGTCCTAG AATGCAAAAC 
TTTCAGGTGG ACAACAACCA TTTCACGGGT GAAATACCTG GAGTGTTCAG CAATTGGTCC 
AACATAGAGT ATGCGTACTT CCATGGCAAT GATCTTAATG GGACGATTCC TGCTTCGCTG 
TCGAACTGCT CAAAGCTGGT ACAATTGCAT TTAAATTCTA ATCCCAAGCT CACCGGGATT 
ATACCTCCAG AGTTCGGAAG GTTGAAGAAA TTGGAGAATT TGGTGGTATT CGACACGAAG 
GTTTACGGGG AGATACCTAC AACGCTTGGG AACTGCTCCA ATCTCAAAAA TCTTGTCCTC 
AATAACAACA CTCTTAACGG GACAATCCCG GCACAGCTTG GAAACTTGAG GAACTTGACA 
CTATTGGTTT TGTCGCAGAA TAAGCTTCAA GGGTCAATTC CGAGCAGTCT CGGCAACTGT 
AAGAATCTTC AGAGACTTGA TCTCTCTAAC AATCGGTTAA CTGGAGGATT GCCAAGTAAT 
CTCTCGGATT GCTTTTCTTT GCAAACAGCT AAACTTGATA ATAATCAGCT CGAAGGAGAC 
TTTGCATGGG ATATGAGCAA CATGACCAAT CTGAAGTCGG TGTCCGTGCG CAACAACTCG 
ATTACAGGAG ATGTGTTCGC ATCACTTGCC ACCCTTAACT CAACGAAAGG CAGCAACCAA 
CTCACGACAT TGGATTTCAC TCGGAACAAC CTAATTGGGA AATTTCCGGC CACATTTGAT 
GTGTCCAAAT TGTCGTCTCT GCAGGTGATG CTGTTAGGAA ACAACCAGCT ACAGGGTTCA 
ATCCCGTCAT GGCTTTGGGA TCTCCCTAAG CTGCAAGTGC TGGAACTCAA TTATAATAAT 
CTCACGGATG ACTTACGAGG CAACTTCAGC AACCCTCGTG GATTTATTGA CGCACCGACT 
ACAACCGATA CTCCTACAGA CAACTCTCAG ACTTACATTC AGAGTGTTAC GATTACTGTC 
CAGGATAGAC AGGTAACGTA CAGTACTTCT CTCAAATATG TCACTTCCAT ACAATTATCG 
CACAACAGAC TGGGGGGCGT CATACCTGTG GATATTACCA AGCTTGTGAA GTTGACGTAT 
CTCGATCTCT CCTCAAACTT CTTCACAGGT AGCATTCCTG ACAATATTGG TGCAATTCCT 
TTGACGTCCC TGAATCTCTC TAGCAATCTT CTGACTGGTA GTATCCCGGA ATCCTTGGGC 
AAAAATGCCA AGCTACAATC TCTGTATCTT GCCAACAACG ACCTATCTGG TCCTATTCCC 
CAAGCATCCC CGTTACAGTC CCAAAATACC TCTGCCTATC GTCCTGGCAA TCCCGGGCTC 
TGTGGCCTTC CTCTTGATCC CTGCAGCTCG ACATCACCAT CATCAGGAAA TTCCTTCAAT 
GATCTTTTCA CCACCCCTGG GTTTATAGTG GGTTTCTTTG TTGGGTTCTT CTCGATCGGG 
TTAATACTCT TCTTTTCTAA GCCTGCTCGA AGCTTTATGA AACTGGAGAA CGGAAGCTCA 
AATAAGGGAC ACACCGGTCG GTGGCATCCC CCAAAGTCCG GCTTGTTGCG AAAAGGGTCC 
CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024364955.1
CDS	514..3399
Translation	MMKTILLLLAVCFLLTASYLTAESFRDEDWDDESEESTDFTKVRQEESERFKRLVSIPNEFDPSLFEGLIHDDFEEDALHEHPGGRQLLQAANILASEQDALLQFKRGVLDPNGILAGWNGTSNQDHCEWVGILCSNITSNNTIRRVVQLNITGVEGAKLGGLISPNISLLSSLTVLILQSNLLTGPIPPELGNLKNLKTLNLHGNNLTSYIPVQLSNLTLLQTLDIGSNNMTGGLPKELAQCKNMLQLDVSSNHFDGEIHSDFGTFPKLKMFLAMNNELTGSIPESFGNCTTLESFAVNDNHLVGEIPRGFANAPNLQGFLVGFNNITGTLPEGFGKLQKLSIVYFQYNKMNGTMDFLQNCSGMWQIHGENNNLTGRLPTFFGDTCENLTHVFVSRNNFSGPLPGTLSKCPKLENIGVSNNNLSGTIPPEFSICPRMQNFQVDNNHFTGEIPGVFSNWSNIEYAYFHGNDLNGTIPASLSNCSKLVQLHLNSNPKLTGIIPPEFGRLKKLENLVVFDTKVYGEIPTTLGNCSNLKNLVLNNNTLNGTIPAQLGNLRNLTLLVLSQNKLQGSIPSSLGNCKNLQRLDLSNNRLTGGLPSNLSDCFSLQTAKLDNNQLEGDFAWDMSNMTNLKSVSVRNNSITGDVFASLATLNSTKGSNQLTTLDFTRNNLIGKFPATFDVSKLSSLQVMLLGNNQLQGSIPSWLWDLPKLQVLELNYNNLTDDLRGNFSNPRGFIDAPTTTDTPTDNSQTYIQSVTITVQDRQVTYSTSLKYVTSIQLSHNRLGGVIPVDITKLVKLTYLDLSSNFFTGSIPDNIGAIPLTSLNLSSNLLTGSIPESLGKNAKLQSLYLANNDLSGPIPQASPLQSQNTSAYRPGNPGLCGLPLDPCSSTSPSSGNSFNDLFTTPGFIVGFFVGFFSIGLILFFSKPARSFMKLENGSSNKGHTGRWHPPKSGLLRKGSL

Target ORF information:

RefSeq Version	XM_024509187.1
Organism	Physcomitrella patens
Definition	Physcomitrella patens probable leucine-rich repeat receptor-like protein kinase At1g35710 (LOC112277155), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024509187.1

ORF Insert Sequence:

ATGATGAAGA CAATCCTTCT CCTGCTGGCA GTTTGCTTCT TGCTTACGGC GTCATATTTG 
ACGGCTGAGA GCTTTAGGGA CGAGGACTGG GACGATGAGT CGGAAGAGTC CACAGATTTT 
ACCAAGGTTC GCCAAGAAGA ATCTGAGAGA TTCAAGAGAC TCGTCTCAAT ACCCAATGAA 
TTCGACCCAT CGCTCTTTGA AGGCTTGATC CATGACGATT TTGAAGAAGA CGCCCTGCAC 
GAGCACCCTG GGGGGCGACA GTTACTTCAA GCTGCCAACA TTCTCGCTAG CGAACAGGAT 
GCGCTCTTGC AGTTTAAGAG AGGAGTACTC GATCCTAATG GTATTCTGGC AGGATGGAAT 
GGAACTTCGA ATCAAGACCA CTGCGAATGG GTAGGCATTC TTTGCAGCAA CATCACGAGT 
AATAACACCA TTCGTCGAGT AGTGCAATTA AATATCACAG GTGTCGAAGG TGCTAAGCTG 
GGAGGTTTGA TTTCCCCGAA CATCTCACTG CTGTCTTCGC TGACAGTCCT AATCCTGCAA 
AGTAACCTAT TAACGGGACC TATTCCCCCA GAGTTGGGGA ATTTGAAAAA TTTGAAGACC 
CTGAATTTAC ATGGCAACAA TCTGACGTCA TACATCCCGG TTCAGCTTTC GAACCTTACG 
CTCCTCCAGA CTCTGGATAT TGGCTCCAAT AATATGACGG GTGGATTGCC AAAAGAGTTA 
GCACAATGTA AGAATATGCT CCAATTGGAT GTGTCTTCAA ATCATTTCGA CGGAGAAATT 
CACAGCGACT TCGGTACTTT TCCAAAGTTG AAAATGTTTT TGGCCATGAA CAACGAGTTA 
ACCGGAAGTA TCCCTGAGAG TTTTGGAAAT TGTACCACTC TGGAGAGCTT TGCGGTGAAT 
GACAATCATT TGGTAGGAGA GATTCCGAGA GGCTTTGCAA ATGCTCCCAA TTTGCAAGGA 
TTTCTGGTGG GGTTTAACAA CATCACCGGA ACGCTTCCTG AGGGGTTTGG GAAGTTGCAG 
AAGTTGAGTA TTGTCTACTT CCAGTATAAC AAGATGAACG GAACCATGGA TTTTCTGCAG 
AATTGCAGCG GAATGTGGCA AATTCATGGC GAGAATAACA ACCTCACTGG GCGACTTCCT 
ACTTTCTTTG GGGATACATG CGAGAACCTG ACGCATGTCT TTGTATCGAG AAACAACTTC 
TCCGGACCAC TTCCGGGTAC CCTATCGAAG TGCCCCAAGT TGGAGAATAT CGGCGTTAGC 
AACAACAATC TCTCCGGCAC CATCCCGCCA GAATTTAGCA TTTGTCCTAG AATGCAAAAC 
TTTCAGGTGG ACAACAACCA TTTCACGGGT GAAATACCTG GAGTGTTCAG CAATTGGTCC 
AACATAGAGT ATGCGTACTT CCATGGCAAT GATCTTAATG GGACGATTCC TGCTTCGCTG 
TCGAACTGCT CAAAGCTGGT ACAATTGCAT TTAAATTCTA ATCCCAAGCT CACCGGGATT 
ATACCTCCAG AGTTCGGAAG GTTGAAGAAA TTGGAGAATT TGGTGGTATT CGACACGAAG 
GTTTACGGGG AGATACCTAC AACGCTTGGG AACTGCTCCA ATCTCAAAAA TCTTGTCCTC 
AATAACAACA CTCTTAACGG GACAATCCCG GCACAGCTTG GAAACTTGAG GAACTTGACA 
CTATTGGTTT TGTCGCAGAA TAAGCTTCAA GGGTCAATTC CGAGCAGTCT CGGCAACTGT 
AAGAATCTTC AGAGACTTGA TCTCTCTAAC AATCGGTTAA CTGGAGGATT GCCAAGTAAT 
CTCTCGGATT GCTTTTCTTT GCAAACAGCT AAACTTGATA ATAATCAGCT CGAAGGAGAC 
TTTGCATGGG ATATGAGCAA CATGACCAAT CTGAAGTCGG TGTCCGTGCG CAACAACTCG 
ATTACAGGAG ATGTGTTCGC ATCACTTGCC ACCCTTAACT CAACGAAAGG CAGCAACCAA 
CTCACGACAT TGGATTTCAC TCGGAACAAC CTAATTGGGA AATTTCCGGC CACATTTGAT 
GTGTCCAAAT TGTCGTCTCT GCAGGTGATG CTGTTAGGAA ACAACCAGCT ACAGGGTTCA 
ATCCCGTCAT GGCTTTGGGA TCTCCCTAAG CTGCAAGTGC TGGAACTCAA TTATAATAAT 
CTCACGGATG ACTTACGAGG CAACTTCAGC AACCCTCGTG GATTTATTGA CGCACCGACT 
ACAACCGATA CTCCTACAGA CAACTCTCAG ACTTACATTC AGAGTGTTAC GATTACTGTC 
CAGGATAGAC AGGTAACGTA CAGTACTTCT CTCAAATATG TCACTTCCAT ACAATTATCG 
CACAACAGAC TGGGGGGCGT CATACCTGTG GATATTACCA AGCTTGTGAA GTTGACGTAT 
CTCGATCTCT CCTCAAACTT CTTCACAGGT AGCATTCCTG ACAATATTGG TGCAATTCCT 
TTGACGTCCC TGAATCTCTC TAGCAATCTT CTGACTGGTA GTATCCCGGA ATCCTTGGGC 
AAAAATGCCA AGCTACAATC TCTGTATCTT GCCAACAACG ACCTATCTGG TCCTATTCCC 
CAAGCATCCC CGTTACAGTC CCAAAATACC TCTGCCTATC GTCCTGGCAA TCCCGGGCTC 
TGTGGCCTTC CTCTTGATCC CTGCAGCTCG ACATCACCAT CATCAGGAAA TTCCTTCAAT 
GATCTTTTCA CCACCCCTGG GTTTATAGTG GGTTTCTTTG TTGGGTTCTT CTCGATCGGG 
TTAATACTCT TCTTTTCTAA GCCTGCTCGA AGCTTTATGA AACTGGAGAA CGGAAGCTCA 
AATAAGGGAC ACACCGGTCG GTGGCATCCC CCAAAGTCCG GCTTGTTGCG AAAAGGGTCC 
CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC112277155 ( XM_024509188.1 ) cDNA ORF clone, -> XP_024364956.1 Physcomitrella patens probable leucine-rich repeat receptor-like protein kinase At1g35710 (LOC112277155), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPn65284
Clone ID Related Accession (Same CDS sequence)	XM_024509188.1
Accession Version	XM_024509188.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2463bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Physcomitrella patens
Product	probable leucine-rich repeat receptor-like protein kinase At1g35710 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037277.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physcomitrella patens Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATGGA ACTTCGAATC AAGACCACTG CGAATGGGTG TCGAAGGTGC TAAGCTGGGA 
GGTTTGATTT CCCCGAACAT CTCACTGCTG TCTTCGCTGA CAGTCCTAAT CCTGCAAAGT 
AACCTATTAA CGGGACCTAT TCCCCCAGAG TTGGGGAATT TGAAAAATTT GAAGACCCTG 
AATTTACATG GCAACAATCT GACGTCATAC ATCCCGGTTC AGCTTTCGAA CCTTACGCTC 
CTCCAGACTC TGGATATTGG CTCCAATAAT ATGACGGGTG GATTGCCAAA AGAGTTAGCA 
CAATGTAAGA ATATGCTCCA ATTGGATGTG TCTTCAAATC ATTTCGACGG AGAAATTCAC 
AGCGACTTCG GTACTTTTCC AAAGTTGAAA ATGTTTTTGG CCATGAACAA CGAGTTAACC 
GGAAGTATCC CTGAGAGTTT TGGAAATTGT ACCACTCTGG AGAGCTTTGC GGTGAATGAC 
AATCATTTGG TAGGAGAGAT TCCGAGAGGC TTTGCAAATG CTCCCAATTT GCAAGGATTT 
CTGGTGGGGT TTAACAACAT CACCGGAACG CTTCCTGAGG GGTTTGGGAA GTTGCAGAAG 
TTGAGTATTG TCTACTTCCA GTATAACAAG ATGAACGGAA CCATGGATTT TCTGCAGAAT 
TGCAGCGGAA TGTGGCAAAT TCATGGCGAG AATAACAACC TCACTGGGCG ACTTCCTACT 
TTCTTTGGGG ATACATGCGA GAACCTGACG CATGTCTTTG TATCGAGAAA CAACTTCTCC 
GGACCACTTC CGGGTACCCT ATCGAAGTGC CCCAAGTTGG AGAATATCGG CGTTAGCAAC 
AACAATCTCT CCGGCACCAT CCCGCCAGAA TTTAGCATTT GTCCTAGAAT GCAAAACTTT 
CAGGTGGACA ACAACCATTT CACGGGTGAA ATACCTGGAG TGTTCAGCAA TTGGTCCAAC 
ATAGAGTATG CGTACTTCCA TGGCAATGAT CTTAATGGGA CGATTCCTGC TTCGCTGTCG 
AACTGCTCAA AGCTGGTACA ATTGCATTTA AATTCTAATC CCAAGCTCAC CGGGATTATA 
CCTCCAGAGT TCGGAAGGTT GAAGAAATTG GAGAATTTGG TGGTATTCGA CACGAAGGTT 
TACGGGGAGA TACCTACAAC GCTTGGGAAC TGCTCCAATC TCAAAAATCT TGTCCTCAAT 
AACAACACTC TTAACGGGAC AATCCCGGCA CAGCTTGGAA ACTTGAGGAA CTTGACACTA 
TTGGTTTTGT CGCAGAATAA GCTTCAAGGG TCAATTCCGA GCAGTCTCGG CAACTGTAAG 
AATCTTCAGA GACTTGATCT CTCTAACAAT CGGTTAACTG GAGGATTGCC AAGTAATCTC 
TCGGATTGCT TTTCTTTGCA AACAGCTAAA CTTGATAATA ATCAGCTCGA AGGAGACTTT 
GCATGGGATA TGAGCAACAT GACCAATCTG AAGTCGGTGT CCGTGCGCAA CAACTCGATT 
ACAGGAGATG TGTTCGCATC ACTTGCCACC CTTAACTCAA CGAAAGGCAG CAACCAACTC 
ACGACATTGG ATTTCACTCG GAACAACCTA ATTGGGAAAT TTCCGGCCAC ATTTGATGTG 
TCCAAATTGT CGTCTCTGCA GGTGATGCTG TTAGGAAACA ACCAGCTACA GGGTTCAATC 
CCGTCATGGC TTTGGGATCT CCCTAAGCTG CAAGTGCTGG AACTCAATTA TAATAATCTC 
ACGGATGACT TACGAGGCAA CTTCAGCAAC CCTCGTGGAT TTATTGACGC ACCGACTACA 
ACCGATACTC CTACAGACAA CTCTCAGACT TACATTCAGA GTGTTACGAT TACTGTCCAG 
GATAGACAGG TAACGTACAG TACTTCTCTC AAATATGTCA CTTCCATACA ATTATCGCAC 
AACAGACTGG GGGGCGTCAT ACCTGTGGAT ATTACCAAGC TTGTGAAGTT GACGTATCTC 
GATCTCTCCT CAAACTTCTT CACAGGTAGC ATTCCTGACA ATATTGGTGC AATTCCTTTG 
ACGTCCCTGA ATCTCTCTAG CAATCTTCTG ACTGGTAGTA TCCCGGAATC CTTGGGCAAA 
AATGCCAAGC TACAATCTCT GTATCTTGCC AACAACGACC TATCTGGTCC TATTCCCCAA 
GCATCCCCGT TACAGTCCCA AAATACCTCT GCCTATCGTC CTGGCAATCC CGGGCTCTGT 
GGCCTTCCTC TTGATCCCTG CAGCTCGACA TCACCATCAT CAGGAAATTC CTTCAATGAT 
CTTTTCACCA CCCCTGGGTT TATAGTGGGT TTCTTTGTTG GGTTCTTCTC GATCGGGTTA 
ATACTCTTCT TTTCTAAGCC TGCTCGAAGC TTTATGAAAC TGGAGAACGG AAGCTCAAAT 
AAGGGACACA CCGGTCGGTG GCATCCCCCA AAGTCCGGCT TGTTGCGAAA AGGGTCCCTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024364956.1
CDS	146..2608
Translation	MEWNFESRPLRMGVEGAKLGGLISPNISLLSSLTVLILQSNLLTGPIPPELGNLKNLKTLNLHGNNLTSYIPVQLSNLTLLQTLDIGSNNMTGGLPKELAQCKNMLQLDVSSNHFDGEIHSDFGTFPKLKMFLAMNNELTGSIPESFGNCTTLESFAVNDNHLVGEIPRGFANAPNLQGFLVGFNNITGTLPEGFGKLQKLSIVYFQYNKMNGTMDFLQNCSGMWQIHGENNNLTGRLPTFFGDTCENLTHVFVSRNNFSGPLPGTLSKCPKLENIGVSNNNLSGTIPPEFSICPRMQNFQVDNNHFTGEIPGVFSNWSNIEYAYFHGNDLNGTIPASLSNCSKLVQLHLNSNPKLTGIIPPEFGRLKKLENLVVFDTKVYGEIPTTLGNCSNLKNLVLNNNTLNGTIPAQLGNLRNLTLLVLSQNKLQGSIPSSLGNCKNLQRLDLSNNRLTGGLPSNLSDCFSLQTAKLDNNQLEGDFAWDMSNMTNLKSVSVRNNSITGDVFASLATLNSTKGSNQLTTLDFTRNNLIGKFPATFDVSKLSSLQVMLLGNNQLQGSIPSWLWDLPKLQVLELNYNNLTDDLRGNFSNPRGFIDAPTTTDTPTDNSQTYIQSVTITVQDRQVTYSTSLKYVTSIQLSHNRLGGVIPVDITKLVKLTYLDLSSNFFTGSIPDNIGAIPLTSLNLSSNLLTGSIPESLGKNAKLQSLYLANNDLSGPIPQASPLQSQNTSAYRPGNPGLCGLPLDPCSSTSPSSGNSFNDLFTTPGFIVGFFVGFFSIGLILFFSKPARSFMKLENGSSNKGHTGRWHPPKSGLLRKGSL

Target ORF information:

RefSeq Version	XM_024509188.1
Organism	Physcomitrella patens
Definition	Physcomitrella patens probable leucine-rich repeat receptor-like protein kinase At1g35710 (LOC112277155), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024509188.1

ORF Insert Sequence:

ATGGAATGGA ACTTCGAATC AAGACCACTG CGAATGGGTG TCGAAGGTGC TAAGCTGGGA 
GGTTTGATTT CCCCGAACAT CTCACTGCTG TCTTCGCTGA CAGTCCTAAT CCTGCAAAGT 
AACCTATTAA CGGGACCTAT TCCCCCAGAG TTGGGGAATT TGAAAAATTT GAAGACCCTG 
AATTTACATG GCAACAATCT GACGTCATAC ATCCCGGTTC AGCTTTCGAA CCTTACGCTC 
CTCCAGACTC TGGATATTGG CTCCAATAAT ATGACGGGTG GATTGCCAAA AGAGTTAGCA 
CAATGTAAGA ATATGCTCCA ATTGGATGTG TCTTCAAATC ATTTCGACGG AGAAATTCAC 
AGCGACTTCG GTACTTTTCC AAAGTTGAAA ATGTTTTTGG CCATGAACAA CGAGTTAACC 
GGAAGTATCC CTGAGAGTTT TGGAAATTGT ACCACTCTGG AGAGCTTTGC GGTGAATGAC 
AATCATTTGG TAGGAGAGAT TCCGAGAGGC TTTGCAAATG CTCCCAATTT GCAAGGATTT 
CTGGTGGGGT TTAACAACAT CACCGGAACG CTTCCTGAGG GGTTTGGGAA GTTGCAGAAG 
TTGAGTATTG TCTACTTCCA GTATAACAAG ATGAACGGAA CCATGGATTT TCTGCAGAAT 
TGCAGCGGAA TGTGGCAAAT TCATGGCGAG AATAACAACC TCACTGGGCG ACTTCCTACT 
TTCTTTGGGG ATACATGCGA GAACCTGACG CATGTCTTTG TATCGAGAAA CAACTTCTCC 
GGACCACTTC CGGGTACCCT ATCGAAGTGC CCCAAGTTGG AGAATATCGG CGTTAGCAAC 
AACAATCTCT CCGGCACCAT CCCGCCAGAA TTTAGCATTT GTCCTAGAAT GCAAAACTTT 
CAGGTGGACA ACAACCATTT CACGGGTGAA ATACCTGGAG TGTTCAGCAA TTGGTCCAAC 
ATAGAGTATG CGTACTTCCA TGGCAATGAT CTTAATGGGA CGATTCCTGC TTCGCTGTCG 
AACTGCTCAA AGCTGGTACA ATTGCATTTA AATTCTAATC CCAAGCTCAC CGGGATTATA 
CCTCCAGAGT TCGGAAGGTT GAAGAAATTG GAGAATTTGG TGGTATTCGA CACGAAGGTT 
TACGGGGAGA TACCTACAAC GCTTGGGAAC TGCTCCAATC TCAAAAATCT TGTCCTCAAT 
AACAACACTC TTAACGGGAC AATCCCGGCA CAGCTTGGAA ACTTGAGGAA CTTGACACTA 
TTGGTTTTGT CGCAGAATAA GCTTCAAGGG TCAATTCCGA GCAGTCTCGG CAACTGTAAG 
AATCTTCAGA GACTTGATCT CTCTAACAAT CGGTTAACTG GAGGATTGCC AAGTAATCTC 
TCGGATTGCT TTTCTTTGCA AACAGCTAAA CTTGATAATA ATCAGCTCGA AGGAGACTTT 
GCATGGGATA TGAGCAACAT GACCAATCTG AAGTCGGTGT CCGTGCGCAA CAACTCGATT 
ACAGGAGATG TGTTCGCATC ACTTGCCACC CTTAACTCAA CGAAAGGCAG CAACCAACTC 
ACGACATTGG ATTTCACTCG GAACAACCTA ATTGGGAAAT TTCCGGCCAC ATTTGATGTG 
TCCAAATTGT CGTCTCTGCA GGTGATGCTG TTAGGAAACA ACCAGCTACA GGGTTCAATC 
CCGTCATGGC TTTGGGATCT CCCTAAGCTG CAAGTGCTGG AACTCAATTA TAATAATCTC 
ACGGATGACT TACGAGGCAA CTTCAGCAAC CCTCGTGGAT TTATTGACGC ACCGACTACA 
ACCGATACTC CTACAGACAA CTCTCAGACT TACATTCAGA GTGTTACGAT TACTGTCCAG 
GATAGACAGG TAACGTACAG TACTTCTCTC AAATATGTCA CTTCCATACA ATTATCGCAC 
AACAGACTGG GGGGCGTCAT ACCTGTGGAT ATTACCAAGC TTGTGAAGTT GACGTATCTC 
GATCTCTCCT CAAACTTCTT CACAGGTAGC ATTCCTGACA ATATTGGTGC AATTCCTTTG 
ACGTCCCTGA ATCTCTCTAG CAATCTTCTG ACTGGTAGTA TCCCGGAATC CTTGGGCAAA 
AATGCCAAGC TACAATCTCT GTATCTTGCC AACAACGACC TATCTGGTCC TATTCCCCAA 
GCATCCCCGT TACAGTCCCA AAATACCTCT GCCTATCGTC CTGGCAATCC CGGGCTCTGT 
GGCCTTCCTC TTGATCCCTG CAGCTCGACA TCACCATCAT CAGGAAATTC CTTCAATGAT 
CTTTTCACCA CCCCTGGGTT TATAGTGGGT TTCTTTGTTG GGTTCTTCTC GATCGGGTTA 
ATACTCTTCT TTTCTAAGCC TGCTCGAAGC TTTATGAAAC TGGAGAACGG AAGCTCAAAT 
AAGGGACACA CCGGTCGGTG GCATCCCCCA AAGTCCGGCT TGTTGCGAAA AGGGTCCCTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC112277155 cDNA ORF clone,

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Gene Symbol	LOC112277155
Entrez Gene ID	112277155