LOC112279271 gene cDNA ORF clone

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPn41280	XM_024513543.1 Latest version!	Physcomitrella patens guanine nucleotide exchange factor SPIKE 1-like (LOC112279271), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPn41280	XM_024513534.1 Latest version!	Physcomitrella patens guanine nucleotide exchange factor SPIKE 1-like (LOC112279271), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPn41280	XM_024513552.1 Latest version!	Physcomitrella patens guanine nucleotide exchange factor SPIKE 1-like (LOC112279271), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPn41280	XM_024513569.1 Latest version!	Physcomitrella patens guanine nucleotide exchange factor SPIKE 1-like (LOC112279271), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPn41280	XM_024513576.1 Latest version!	Physcomitrella patens guanine nucleotide exchange factor SPIKE 1-like (LOC112279271), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPn41280	XM_024513560.1 Latest version!	Physcomitrella patens guanine nucleotide exchange factor SPIKE 1-like (LOC112279271), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPn41280	XM_024513585.1 Latest version!	Physcomitrella patens guanine nucleotide exchange factor SPIKE 1-like (LOC112279271), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPn41281	XM_024513595.1 Latest version!	Physcomitrella patens guanine nucleotide exchange factor SPIKE 1-like (LOC112279271), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPn41282	XM_024513604.1 Latest version!	Physcomitrella patens guanine nucleotide exchange factor SPIKE 1-like (LOC112279271), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPn41283	XM_024513613.1 Latest version!	Physcomitrella patens guanine nucleotide exchange factor SPIKE 1-like (LOC112279271), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPn41284	XM_024513623.1 Latest version!	Physcomitrella patens guanine nucleotide exchange factor SPIKE 1-like (LOC112279271), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPn41285	XM_024513632.1 Latest version!	Physcomitrella patens guanine nucleotide exchange factor SPIKE 1-like (LOC112279271), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPn41286	XM_024513635.1 Latest version!	Physcomitrella patens guanine nucleotide exchange factor SPIKE 1-like (LOC112279271), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

LOC112279271 ( XM_024513560.1 ) cDNA ORF clone, -> XP_024369328.1 Physcomitrella patens guanine nucleotide exchange factor SPIKE 1-like (LOC112279271), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OPn41280
Clone ID Related Accession (Same CDS sequence)	XM_024513560.1 , XM_024513543.1 , XM_024513534.1 , XM_024513569.1 , XM_024513576.1 , XM_024513552.1 , XM_024513585.1
Accession Version	XM_024513560.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Physcomitrella patens
Product	guanine nucleotide exchange factor SPIKE 1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037253.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physcomitrella patens Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATTTGG TCGCTGGAGC GCCTATATGG TCGGTGGAAA GCGTCAAGGA GGGAAAAGGA 
TCCAGGTTCG ACGTGTCGGA CTCAGTAGGA CAATGTAGGG TAGGTAGTCA TGATCGGAGG 
GACGAGGAGG AGACATGGGG ACTCAATGCA GAGACAGAAA GCTCTGCATT CTGGGAGCTG 
AGCTTCGAGA CTAGAGCTGT CGTCGACAGC GGATCAATTA AGAAGCAGGG GATGGATGAA 
ATTGCCTTGA GCGGGCAGCG GTTCAAACGT ATCCCACACC CGTACATCGG GCAGTCAACA 
GATCTTGAGT CCTTGCTGAA TGGGAATGGA GACCCTTTCC CACATTTCAA AGAGCTTCTT 
CAAAGCTACA GGGCTGAATG GGTGAAGGAT GAGTTCAAAT ACGGTCATTA TGAAGCTGTG 
CCACCGCCAG CCTTAGAACA TCAGATTTTT GAAGGACCCG ACACTGACAT ACAGACAGAG 
TTTCGGCTTT CTAACGTCAG ACTCGGCAGA AATGGTGATG TTACTGATGA TGAAGATCCT 
CAAACTCCAC GACGGCTTTC AAGACGTTTT TCTGTACGGT CATCTGGTCG TTTCTCTATG 
AGATCTACGA CTAGTGATGC ACCTGAAGCA TTGCACACAA ATATCTCAAA GCATTATGGG 
GTTGCTCCGT TACCTCCTTA TGAGCCCGTT TATAATTGGC GAGCAACAAG GGCATCTGTA 
CTTGGACAAA GAACTTCGGA ACTTCCATCA GCAATATCAT CTAGTAGCGG GCTGAAGGTT 
TCTGTGAAGG TTGTCTCTTT GAATGTTCAG GCCGGACTTG TCGAACCCAT GTACGGTACC 
TTGTGTCTCT ATCATCGAGA GAAGCGGGAG AAATTATCAG AAAATTTTCA CTTCCGATGT 
TTACCATCAG AGTTCCAAGA TTGTGTAACT TGGCAGGAGG GTGGTAATTG TCTGAGAAGA 
GCTATATTTT CACTGGAGGC AGCGTCGCCA GCAATATGTT TGTTGATCCA GCTTGAAAAG 
CATGTGACTG AGGAAGGAGG AGTAACGGCT CATGTTTATT CTCGAAAAGA ACCTGTACAC 
TTGACCGAGA GAGAAAAGCA GAAGTTACAG GTCTGGGCGC GCGTGATGCC ATTCAGGGAG 
CCTTTCGCGT GGGCAGTAGT TGCTCTGTTT GATGCAAATA TAACAGGAGG TGTAGGAGGA 
TTCTCATCTT CTCCAAGCAG CCCACTTCCA CCAGGACTTT TTGGACCATC TTTGATGGAA 
GCATCCTTGG ATTCTGATGG ACGACTTATA GCTGATGCTA AGACAGAATC ATCTAGTGTG 
CCAGTTTTGG TTGATGTTCA GAGCCTTCGC CGGGTCAAAG AAAATTACAC GGAGGAAATG 
CTTCAGGATC CCAAGTACAA AGTGCACAAA CCCGTGAAGG CCATCCTTCG TCTCGAGATA 
GAGAGGTTAT CTACTGAAGA TCATGACCAA GATTCCATAT TTGAGTGTGG TAGTTTTGGT 
CGTGGGTCCA CTGATGGTGA TTCCCGCTTG ACAGTAGGAC CCTCTTTCTC AGGGCGTTTC 
ATGCAGGGGC TGGGCATGAC GAAGGTTACG CATAATGGGC GGCAGAAATG GAGCCCTGGA 
AGAAAACATC GCTATCTGCA TTCCGGCAGT GTTGGCAGCA TTGAATTTGG AAAGTTTGAC 
TTCCGGGCTA TTGAGTTTCG ATCGGGTTCG AAAGTTGAGG TTTTACCTCA GCTATTGCAC 
TGCCTGTATG TATATCCTCA ATCAGTCAAT TTAAGTCGAA AACGCAATCT TTTTGTGCGA 
GTGGAGCTTC GAAAGGATGA TGTAGACATT CGCAAATCTC CTCTCGAGGC CCTTTATCCG 
AGGGATGCTG ACAGCTCTAT GCAGAAATAT GCACACTCTC AGATCGATGC AAACACCAAG 
ACACCTCACT ATCATGATGA GTTCAAAATT CAACTACCTG CCGTTCTCTC TCCTGAGCAT 
CACCTTCTGT TCACGTTTTT TCATGTGGAT CTACAGATGA AAATTGAGGC TCCAAAGCCG 
GTGGTAGTGG GATACTCTGT TCTTCCGCTC ATGTTTGTTT GTCAGGTTCA GCGGTTGGAC 
GGCAGCCTAC CTATCGCAAA GGAGCTGTAT TCCAATTATC TACAAGAAAA TGTGAAGGAA 
AGGTTGGAGT ACTTGGATGA TGGCAAAACT GTATTTAAAT TGAGGTCACG ATTATGCTCT 
TCTCTTTATC CGGTTAATGA GAGGATCCGA GATTTCTTTT CAGAATACGA TCGACATGTC 
TTGCGGACCA GTCCACCCTG GGGTACTGAG CTGATGGAGG CTATTAATGC TCTGAAGACG 
GTTGAGCCGT CAGCTATGCT CCAGTTTCTT CAACCTATTC TCAACATGCT GCTTCGTATG 
ATTCGCGATG GAGGAGAAAC GTTGCAGGTT GCTGCATTCC GTTCAATGGT TAACATCATT 
ACCAGGGTAC AAGCAGAGAC ATCTGATGGG GCTGAAAGAA ATCGTTACCT TGTGCATTAT 
GTGGATTATG CATTTAATGA TTTTGGTGGG CGTCATGAGC CTGTGTACCC AGGTCTGTGC 
AGTGTATGGC GCAGCCTTGC TCGAAGCAAG GCGAAAGGCT ACCGTGTCGG ACCTGTGTAT 
GATGATGTTC TTTCCATGGC TTGGGTGTTC CTGGAGCTAA TTGTCAAGTC CATGGCACTT 
GAACAATCCC GCATTTACTC TGAGTCCCTC CCACCTGGAG AGGAGTTACC ACCTTTGCAG 
CTCATTGATG AGGTCTTTAA AAGTATCGTA CAGCTCTATG ATTGTTTACT GACAGAGGTT 
CATGAGCGCT GCAAGAAGGG CATGCTTCTC GCAATGAAGC TGAATAGCAG TATTGCATAC 
TTTTGCTATG ACCTTCTCTC TGTAATTGAG CATCGACAAG TCTTTGAGCT GGTTGCGCTT 
TATTTCAACA AGTTTGCTGG GGTATGTCAA TCTCTTGTGC ACGAATGTAA GCTTAATTTT 
CTGCGCATCG TCTGTGACCA TGATCTCTTT GTTGAGATGC CCGGTCGAGA TCCTACAGAG 
AGGAATTATT TGGCATCACT GTTGATGCAA GAGCTCTTTG TTACTTGGGA TCATGAAGAT 
CTGACTTTAA GATCTAAGGC AGCTCGAATC CTTGTGCAAC TTATGTGCAA GCATGAGTAT 
GATGCACGGT ATCAAACATT GGAGGACAAA ATGTATATTG CCCAACGTTA CTTTCCACTA 
GTTGACCTGA TATTGGACGA AATGCCAGTG TTCTATGGCC TGAGCACTAC AGAGAAGCGT 
GAAGTGCTGG TGTGTGTGCT TCACATTCTG CGATATCTAG ACGATGCATC TCTTGTTAAA 
GCTTGGCAGC ACAACATGGA TAGAACAAAG CTTTTTTTCA AACTCCTGGA AGAATGTCAG 
GAATTGCTGG AGTATAAGAA GGCTGGAGCA GATGGCTTGA TGGGAGCTAT GCCAGCGAAT 
GATCAAGGAG AAGGGCCCCT TCGATACTCC GAGAAGCTCT CACCTGCAGT CAATCAGTTT 
TTGATTGAGG CATCTCGGCA GGATGTCAGA CTCACAAAGA TTTCTCCAAA TGCTCAGTGG 
ATACTTCCAC AAACGAGTCC AGAGATTGGT TCATTCTGGA AAAGAGCAAG CCCTGTAACA 
AATAGTTCAA CTAGCCCAGG TCAGCCGCAT TCGTTGCGAG AGGCACTAGC CCAAGCACAG 
TCTGCGGGAA GAAGTGGAGG CAGGTCTCTT CGTGAGACCT TGCATCCTAT GCTCCGCCAA 
AAGTTGGAGC TATGGGAGGA GAACCTTAGT GCCTCTGCTA TGCTCCAAAT TTTGGAGGTA 
CTTGAGAAGT TTATAAGTGC AGTATCAAGT CAAGCTGTTC TCACTGACTA CATGAGGCTT 
GATTGTATCA CAACTATTTT TATTGGTTTT CTGGGACGCA GCCAGCCGTT GCCATTTTGG 
AAGGCCTTTC TACCTTTCTT CAATAACGTG TTTCGTCGTC ATGGCGCTAC CTTGATGAGT 
AGGGAGAATG ATAGGTTCTT GAAGCAAGTA GCATTTCATC TACTACGGCT CGGAGTTTTC 
CGGAATGAGT CCATACGGAA ACGTTCTGTT GTTGGCCTTC AAATTCTTGT CCGAAATGCA 
TACCACTACT TTCAAGGCCT TTCAAGACTG CGGGTGATGC TTACAATCAC ACTTTCTGAG 
CTCATGTCAG ATGTTCAAGT AACACATCCT AAGATGGATG GATCCTTGGA AGAGAGTGGT 
GAATCTCAAC GTCTTCGTAG GTCCTTGCAA CAGATTGCAC AAGAAAGTAT TAGTGCGGAT 
CTTCTACGGG AATGTGGTCT TCCTGAAGAT TCTCTTACTG CTTTGCCAGA AGGTGTGAAT 
GGCCAACAAT GGAGTTGGGC TGGAGTCGCT GAGCTTTCCA CCTCTCTGCT GAAAGCTGTG 
GATGCTGCTG TAGGACATGC TCTTTTGGGT CCTGAGATTT TAATGAATGA CAAGTATGCA 
ACTGCAGAGG CCTACCATGG TTTGGCGACG GCATATACCA ACGTGCCCGA TCTGCATATA 
ATGTGGCTTC TGTATCTCTG CGAAGTTCAT CAAGGCAACC AGTCTTATGC AGAGGCTGCT 
CAGTGTGCTG TTGCGGTTGC AGGTGTCATC ATGCATGCTA TAGTAGGAAA AGGTGATCAT 
GTTTGGGGAA AAGAGCATGT TGAGGCACTG GGTAATATTT TCCCAATGCT CACTGGTTCC 
CGCTTTGGGG ATGCTGCAAC TGCCGAAATT GAAGGCTATG GGTCATCGAA ATTGACTGTC 
GAATCGGCAG TGAAGTACTT GCAGCTCGCC AATAAACTCT TTGTCCAGGC AGAGCTCTTC 
CATTTCTGTG CCGGCATATT GGAGCTCATA ATTCCCGTCC ATAAGTCAAG GCGTGCCTAT 
AGTCAGCTGT CTAAATGTCA TACTTCATTG ACCAGCATCT ACGAAGCTAT ACTAGAGCAA 
GAGTCGAGCC CTATCCCTTT CACGGATGCA ACATATTATC GTGTGGGATT TTATGGGAAA 
AGCTTTGGTA GTTTGCACTG CAAGGAGTAT GTGTACAGGG AACCACGGGA CGTGCGGTTA 
GGGGACATTA TGAAAAATTT AGGAAATATT TATGAGCCTA CAGTAATTGA AGGAAAGCAG 
AACTTGCATA TTATCCCCGA TTCTCGTCAA GTGAATCCAG AGGATCTTCA AGCTGGCATT 
TGTTACTTGC AAATTACATC TGTCGATCCT GTTACAGAGG ACGAGGATTT GGAAAGCAGG 
CGAGAAAGAC AATCCGGGAG GTCCTCGTCA ACATTCAGTG CACGAGTCTT CGATCGATTT 
TTATTTGACA CTCCTTTCAC AAAGAATGGA AGATCCCAAG GAGGTTTGGA AGATCAGTGG 
AAGAGGCGTA CGGTTTTGTG GACTGAAGGG CCTTTTCCAT CTCTTGTAAA TCGTTTAATG 
GTTGTGAAGT CTGAGTCGCG AGAATTTTCG CCAATCGAAA ACGCCATAGG AATGATTGAG 
ACTCGTACAG CAGCTTTAGC AGGAGAGCTC GATGATCAAC GTATAAATGA GGGAGATCCT 
CTGCCCCGTT TACAAGGCCT GCAAAGAATC TTGCAAGGAA GTGTTGCAGT CCAGGTAAAT 
AGTGGAGTGC TAGGTGTCTG CACAGCTTTC TTGTCTGGGG AACCTTCAAC ACGATTGCGC 
TCACAAGAAC TACAGCAGCT GATAGCAGCA CTCTTGGAGT TTATGGCTGT GTGCAAGAAA 
GCAATTCGAG TACATGCACG ACTGATTGGA GAGGAGGACC AAGACTTCCA CTCGCAGCTT 
GTGATTGGAT TCCAATCACT CACAGCAGAG CTATCCCACT ATATTCCCGC CATCTTGTCA 
GATGTAACCA TACTTGGGAA ACACAGCAAT GTGCTCAAGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024369328.1
CDS	466..6390
Translation	MHLVAGAPIWSVESVKEGKGSRFDVSDSVGQCRVGSHDRRDEEETWGLNAETESSAFWELSFETRAVVDSGSIKKQGMDEIALSGQRFKRIPHPYIGQSTDLESLLNGNGDPFPHFKELLQSYRAEWVKDEFKYGHYEAVPPPALEHQIFEGPDTDIQTEFRLSNVRLGRNGDVTDDEDPQTPRRLSRRFSVRSSGRFSMRSTTSDAPEALHTNISKHYGVAPLPPYEPVYNWRATRASVLGQRTSELPSAISSSSGLKVSVKVVSLNVQAGLVEPMYGTLCLYHREKREKLSENFHFRCLPSEFQDCVTWQEGGNCLRRAIFSLEAASPAICLLIQLEKHVTEEGGVTAHVYSRKEPVHLTEREKQKLQVWARVMPFREPFAWAVVALFDANITGGVGGFSSSPSSPLPPGLFGPSLMEASLDSDGRLIADAKTESSSVPVLVDVQSLRRVKENYTEEMLQDPKYKVHKPVKAILRLEIERLSTEDHDQDSIFECGSFGRGSTDGDSRLTVGPSFSGRFMQGLGMTKVTHNGRQKWSPGRKHRYLHSGSVGSIEFGKFDFRAIEFRSGSKVEVLPQLLHCLYVYPQSVNLSRKRNLFVRVELRKDDVDIRKSPLEALYPRDADSSMQKYAHSQIDANTKTPHYHDEFKIQLPAVLSPEHHLLFTFFHVDLQMKIEAPKPVVVGYSVLPLMFVCQVQRLDGSLPIAKELYSNYLQENVKERLEYLDDGKTVFKLRSRLCSSLYPVNERIRDFFSEYDRHVLRTSPPWGTELMEAINALKTVEPSAMLQFLQPILNMLLRMIRDGGETLQVAAFRSMVNIITRVQAETSDGAERNRYLVHYVDYAFNDFGGRHEPVYPGLCSVWRSLARSKAKGYRVGPVYDDVLSMAWVFLELIVKSMALEQSRIYSESLPPGEELPPLQLIDEVFKSIVQLYDCLLTEVHERCKKGMLLAMKLNSSIAYFCYDLLSVIEHRQVFELVALYFNKFAGVCQSLVHECKLNFLRIVCDHDLFVEMPGRDPTERNYLASLLMQELFVTWDHEDLTLRSKAARILVQLMCKHEYDARYQTLEDKMYIAQRYFPLVDLILDEMPVFYGLSTTEKREVLVCVLHILRYLDDASLVKAWQHNMDRTKLFFKLLEECQELLEYKKAGADGLMGAMPANDQGEGPLRYSEKLSPAVNQFLIEASRQDVRLTKISPNAQWILPQTSPEIGSFWKRASPVTNSSTSPGQPHSLREALAQAQSAGRSGGRSLRETLHPMLRQKLELWEENLSASAMLQILEVLEKFISAVSSQAVLTDYMRLDCITTIFIGFLGRSQPLPFWKAFLPFFNNVFRRHGATLMSRENDRFLKQVAFHLLRLGVFRNESIRKRSVVGLQILVRNAYHYFQGLSRLRVMLTITLSELMSDVQVTHPKMDGSLEESGESQRLRRSLQQIAQESISADLLRECGLPEDSLTALPEGVNGQQWSWAGVAELSTSLLKAVDAAVGHALLGPEILMNDKYATAEAYHGLATAYTNVPDLHIMWLLYLCEVHQGNQSYAEAAQCAVAVAGVIMHAIVGKGDHVWGKEHVEALGNIFPMLTGSRFGDAATAEIEGYGSSKLTVESAVKYLQLANKLFVQAELFHFCAGILELIIPVHKSRRAYSQLSKCHTSLTSIYEAILEQESSPIPFTDATYYRVGFYGKSFGSLHCKEYVYREPRDVRLGDIMKNLGNIYEPTVIEGKQNLHIIPDSRQVNPEDLQAGICYLQITSVDPVTEDEDLESRRERQSGRSSSTFSARVFDRFLFDTPFTKNGRSQGGLEDQWKRRTVLWTEGPFPSLVNRLMVVKSESREFSPIENAIGMIETRTAALAGELDDQRINEGDPLPRLQGLQRILQGSVAVQVNSGVLGVCTAFLSGEPSTRLRSQELQQLIAALLEFMAVCKKAIRVHARLIGEEDQDFHSQLVIGFQSLTAELSHYIPAILSDVTILGKHSNVLKL

Target ORF information:

RefSeq Version	XM_024513560.1
Organism	Physcomitrella patens
Definition	Physcomitrella patens guanine nucleotide exchange factor SPIKE 1-like (LOC112279271), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024513560.1

ORF Insert Sequence:

ATGCATTTGG TCGCTGGAGC GCCTATATGG TCGGTGGAAA GCGTCAAGGA GGGAAAAGGA 
TCCAGGTTCG ACGTGTCGGA CTCAGTAGGA CAATGTAGGG TAGGTAGTCA TGATCGGAGG 
GACGAGGAGG AGACATGGGG ACTCAATGCA GAGACAGAAA GCTCTGCATT CTGGGAGCTG 
AGCTTCGAGA CTAGAGCTGT CGTCGACAGC GGATCAATTA AGAAGCAGGG GATGGATGAA 
ATTGCCTTGA GCGGGCAGCG GTTCAAACGT ATCCCACACC CGTACATCGG GCAGTCAACA 
GATCTTGAGT CCTTGCTGAA TGGGAATGGA GACCCTTTCC CACATTTCAA AGAGCTTCTT 
CAAAGCTACA GGGCTGAATG GGTGAAGGAT GAGTTCAAAT ACGGTCATTA TGAAGCTGTG 
CCACCGCCAG CCTTAGAACA TCAGATTTTT GAAGGACCCG ACACTGACAT ACAGACAGAG 
TTTCGGCTTT CTAACGTCAG ACTCGGCAGA AATGGTGATG TTACTGATGA TGAAGATCCT 
CAAACTCCAC GACGGCTTTC AAGACGTTTT TCTGTACGGT CATCTGGTCG TTTCTCTATG 
AGATCTACGA CTAGTGATGC ACCTGAAGCA TTGCACACAA ATATCTCAAA GCATTATGGG 
GTTGCTCCGT TACCTCCTTA TGAGCCCGTT TATAATTGGC GAGCAACAAG GGCATCTGTA 
CTTGGACAAA GAACTTCGGA ACTTCCATCA GCAATATCAT CTAGTAGCGG GCTGAAGGTT 
TCTGTGAAGG TTGTCTCTTT GAATGTTCAG GCCGGACTTG TCGAACCCAT GTACGGTACC 
TTGTGTCTCT ATCATCGAGA GAAGCGGGAG AAATTATCAG AAAATTTTCA CTTCCGATGT 
TTACCATCAG AGTTCCAAGA TTGTGTAACT TGGCAGGAGG GTGGTAATTG TCTGAGAAGA 
GCTATATTTT CACTGGAGGC AGCGTCGCCA GCAATATGTT TGTTGATCCA GCTTGAAAAG 
CATGTGACTG AGGAAGGAGG AGTAACGGCT CATGTTTATT CTCGAAAAGA ACCTGTACAC 
TTGACCGAGA GAGAAAAGCA GAAGTTACAG GTCTGGGCGC GCGTGATGCC ATTCAGGGAG 
CCTTTCGCGT GGGCAGTAGT TGCTCTGTTT GATGCAAATA TAACAGGAGG TGTAGGAGGA 
TTCTCATCTT CTCCAAGCAG CCCACTTCCA CCAGGACTTT TTGGACCATC TTTGATGGAA 
GCATCCTTGG ATTCTGATGG ACGACTTATA GCTGATGCTA AGACAGAATC ATCTAGTGTG 
CCAGTTTTGG TTGATGTTCA GAGCCTTCGC CGGGTCAAAG AAAATTACAC GGAGGAAATG 
CTTCAGGATC CCAAGTACAA AGTGCACAAA CCCGTGAAGG CCATCCTTCG TCTCGAGATA 
GAGAGGTTAT CTACTGAAGA TCATGACCAA GATTCCATAT TTGAGTGTGG TAGTTTTGGT 
CGTGGGTCCA CTGATGGTGA TTCCCGCTTG ACAGTAGGAC CCTCTTTCTC AGGGCGTTTC 
ATGCAGGGGC TGGGCATGAC GAAGGTTACG CATAATGGGC GGCAGAAATG GAGCCCTGGA 
AGAAAACATC GCTATCTGCA TTCCGGCAGT GTTGGCAGCA TTGAATTTGG AAAGTTTGAC 
TTCCGGGCTA TTGAGTTTCG ATCGGGTTCG AAAGTTGAGG TTTTACCTCA GCTATTGCAC 
TGCCTGTATG TATATCCTCA ATCAGTCAAT TTAAGTCGAA AACGCAATCT TTTTGTGCGA 
GTGGAGCTTC GAAAGGATGA TGTAGACATT CGCAAATCTC CTCTCGAGGC CCTTTATCCG 
AGGGATGCTG ACAGCTCTAT GCAGAAATAT GCACACTCTC AGATCGATGC AAACACCAAG 
ACACCTCACT ATCATGATGA GTTCAAAATT CAACTACCTG CCGTTCTCTC TCCTGAGCAT 
CACCTTCTGT TCACGTTTTT TCATGTGGAT CTACAGATGA AAATTGAGGC TCCAAAGCCG 
GTGGTAGTGG GATACTCTGT TCTTCCGCTC ATGTTTGTTT GTCAGGTTCA GCGGTTGGAC 
GGCAGCCTAC CTATCGCAAA GGAGCTGTAT TCCAATTATC TACAAGAAAA TGTGAAGGAA 
AGGTTGGAGT ACTTGGATGA TGGCAAAACT GTATTTAAAT TGAGGTCACG ATTATGCTCT 
TCTCTTTATC CGGTTAATGA GAGGATCCGA GATTTCTTTT CAGAATACGA TCGACATGTC 
TTGCGGACCA GTCCACCCTG GGGTACTGAG CTGATGGAGG CTATTAATGC TCTGAAGACG 
GTTGAGCCGT CAGCTATGCT CCAGTTTCTT CAACCTATTC TCAACATGCT GCTTCGTATG 
ATTCGCGATG GAGGAGAAAC GTTGCAGGTT GCTGCATTCC GTTCAATGGT TAACATCATT 
ACCAGGGTAC AAGCAGAGAC ATCTGATGGG GCTGAAAGAA ATCGTTACCT TGTGCATTAT 
GTGGATTATG CATTTAATGA TTTTGGTGGG CGTCATGAGC CTGTGTACCC AGGTCTGTGC 
AGTGTATGGC GCAGCCTTGC TCGAAGCAAG GCGAAAGGCT ACCGTGTCGG ACCTGTGTAT 
GATGATGTTC TTTCCATGGC TTGGGTGTTC CTGGAGCTAA TTGTCAAGTC CATGGCACTT 
GAACAATCCC GCATTTACTC TGAGTCCCTC CCACCTGGAG AGGAGTTACC ACCTTTGCAG 
CTCATTGATG AGGTCTTTAA AAGTATCGTA CAGCTCTATG ATTGTTTACT GACAGAGGTT 
CATGAGCGCT GCAAGAAGGG CATGCTTCTC GCAATGAAGC TGAATAGCAG TATTGCATAC 
TTTTGCTATG ACCTTCTCTC TGTAATTGAG CATCGACAAG TCTTTGAGCT GGTTGCGCTT 
TATTTCAACA AGTTTGCTGG GGTATGTCAA TCTCTTGTGC ACGAATGTAA GCTTAATTTT 
CTGCGCATCG TCTGTGACCA TGATCTCTTT GTTGAGATGC CCGGTCGAGA TCCTACAGAG 
AGGAATTATT TGGCATCACT GTTGATGCAA GAGCTCTTTG TTACTTGGGA TCATGAAGAT 
CTGACTTTAA GATCTAAGGC AGCTCGAATC CTTGTGCAAC TTATGTGCAA GCATGAGTAT 
GATGCACGGT ATCAAACATT GGAGGACAAA ATGTATATTG CCCAACGTTA CTTTCCACTA 
GTTGACCTGA TATTGGACGA AATGCCAGTG TTCTATGGCC TGAGCACTAC AGAGAAGCGT 
GAAGTGCTGG TGTGTGTGCT TCACATTCTG CGATATCTAG ACGATGCATC TCTTGTTAAA 
GCTTGGCAGC ACAACATGGA TAGAACAAAG CTTTTTTTCA AACTCCTGGA AGAATGTCAG 
GAATTGCTGG AGTATAAGAA GGCTGGAGCA GATGGCTTGA TGGGAGCTAT GCCAGCGAAT 
GATCAAGGAG AAGGGCCCCT TCGATACTCC GAGAAGCTCT CACCTGCAGT CAATCAGTTT 
TTGATTGAGG CATCTCGGCA GGATGTCAGA CTCACAAAGA TTTCTCCAAA TGCTCAGTGG 
ATACTTCCAC AAACGAGTCC AGAGATTGGT TCATTCTGGA AAAGAGCAAG CCCTGTAACA 
AATAGTTCAA CTAGCCCAGG TCAGCCGCAT TCGTTGCGAG AGGCACTAGC CCAAGCACAG 
TCTGCGGGAA GAAGTGGAGG CAGGTCTCTT CGTGAGACCT TGCATCCTAT GCTCCGCCAA 
AAGTTGGAGC TATGGGAGGA GAACCTTAGT GCCTCTGCTA TGCTCCAAAT TTTGGAGGTA 
CTTGAGAAGT TTATAAGTGC AGTATCAAGT CAAGCTGTTC TCACTGACTA CATGAGGCTT 
GATTGTATCA CAACTATTTT TATTGGTTTT CTGGGACGCA GCCAGCCGTT GCCATTTTGG 
AAGGCCTTTC TACCTTTCTT CAATAACGTG TTTCGTCGTC ATGGCGCTAC CTTGATGAGT 
AGGGAGAATG ATAGGTTCTT GAAGCAAGTA GCATTTCATC TACTACGGCT CGGAGTTTTC 
CGGAATGAGT CCATACGGAA ACGTTCTGTT GTTGGCCTTC AAATTCTTGT CCGAAATGCA 
TACCACTACT TTCAAGGCCT TTCAAGACTG CGGGTGATGC TTACAATCAC ACTTTCTGAG 
CTCATGTCAG ATGTTCAAGT AACACATCCT AAGATGGATG GATCCTTGGA AGAGAGTGGT 
GAATCTCAAC GTCTTCGTAG GTCCTTGCAA CAGATTGCAC AAGAAAGTAT TAGTGCGGAT 
CTTCTACGGG AATGTGGTCT TCCTGAAGAT TCTCTTACTG CTTTGCCAGA AGGTGTGAAT 
GGCCAACAAT GGAGTTGGGC TGGAGTCGCT GAGCTTTCCA CCTCTCTGCT GAAAGCTGTG 
GATGCTGCTG TAGGACATGC TCTTTTGGGT CCTGAGATTT TAATGAATGA CAAGTATGCA 
ACTGCAGAGG CCTACCATGG TTTGGCGACG GCATATACCA ACGTGCCCGA TCTGCATATA 
ATGTGGCTTC TGTATCTCTG CGAAGTTCAT CAAGGCAACC AGTCTTATGC AGAGGCTGCT 
CAGTGTGCTG TTGCGGTTGC AGGTGTCATC ATGCATGCTA TAGTAGGAAA AGGTGATCAT 
GTTTGGGGAA AAGAGCATGT TGAGGCACTG GGTAATATTT TCCCAATGCT CACTGGTTCC 
CGCTTTGGGG ATGCTGCAAC TGCCGAAATT GAAGGCTATG GGTCATCGAA ATTGACTGTC 
GAATCGGCAG TGAAGTACTT GCAGCTCGCC AATAAACTCT TTGTCCAGGC AGAGCTCTTC 
CATTTCTGTG CCGGCATATT GGAGCTCATA ATTCCCGTCC ATAAGTCAAG GCGTGCCTAT 
AGTCAGCTGT CTAAATGTCA TACTTCATTG ACCAGCATCT ACGAAGCTAT ACTAGAGCAA 
GAGTCGAGCC CTATCCCTTT CACGGATGCA ACATATTATC GTGTGGGATT TTATGGGAAA 
AGCTTTGGTA GTTTGCACTG CAAGGAGTAT GTGTACAGGG AACCACGGGA CGTGCGGTTA 
GGGGACATTA TGAAAAATTT AGGAAATATT TATGAGCCTA CAGTAATTGA AGGAAAGCAG 
AACTTGCATA TTATCCCCGA TTCTCGTCAA GTGAATCCAG AGGATCTTCA AGCTGGCATT 
TGTTACTTGC AAATTACATC TGTCGATCCT GTTACAGAGG ACGAGGATTT GGAAAGCAGG 
CGAGAAAGAC AATCCGGGAG GTCCTCGTCA ACATTCAGTG CACGAGTCTT CGATCGATTT 
TTATTTGACA CTCCTTTCAC AAAGAATGGA AGATCCCAAG GAGGTTTGGA AGATCAGTGG 
AAGAGGCGTA CGGTTTTGTG GACTGAAGGG CCTTTTCCAT CTCTTGTAAA TCGTTTAATG 
GTTGTGAAGT CTGAGTCGCG AGAATTTTCG CCAATCGAAA ACGCCATAGG AATGATTGAG 
ACTCGTACAG CAGCTTTAGC AGGAGAGCTC GATGATCAAC GTATAAATGA GGGAGATCCT 
CTGCCCCGTT TACAAGGCCT GCAAAGAATC TTGCAAGGAA GTGTTGCAGT CCAGGTAAAT 
AGTGGAGTGC TAGGTGTCTG CACAGCTTTC TTGTCTGGGG AACCTTCAAC ACGATTGCGC 
TCACAAGAAC TACAGCAGCT GATAGCAGCA CTCTTGGAGT TTATGGCTGT GTGCAAGAAA 
GCAATTCGAG TACATGCACG ACTGATTGGA GAGGAGGACC AAGACTTCCA CTCGCAGCTT 
GTGATTGGAT TCCAATCACT CACAGCAGAG CTATCCCACT ATATTCCCGC CATCTTGTCA 
GATGTAACCA TACTTGGGAA ACACAGCAAT GTGCTCAAGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC112279271 ( XM_024513543.1 ) cDNA ORF clone, -> XP_024369311.1 Physcomitrella patens guanine nucleotide exchange factor SPIKE 1-like (LOC112279271), transcript variant X2, mRNA.

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CloneID	OPn41280
Clone ID Related Accession (Same CDS sequence)	XM_024513560.1 , XM_024513543.1 , XM_024513534.1 , XM_024513569.1 , XM_024513576.1 , XM_024513552.1 , XM_024513585.1
Accession Version	XM_024513543.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Physcomitrella patens
Product	guanine nucleotide exchange factor SPIKE 1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037253.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physcomitrella patens Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATTTGG TCGCTGGAGC GCCTATATGG TCGGTGGAAA GCGTCAAGGA GGGAAAAGGA 
TCCAGGTTCG ACGTGTCGGA CTCAGTAGGA CAATGTAGGG TAGGTAGTCA TGATCGGAGG 
GACGAGGAGG AGACATGGGG ACTCAATGCA GAGACAGAAA GCTCTGCATT CTGGGAGCTG 
AGCTTCGAGA CTAGAGCTGT CGTCGACAGC GGATCAATTA AGAAGCAGGG GATGGATGAA 
ATTGCCTTGA GCGGGCAGCG GTTCAAACGT ATCCCACACC CGTACATCGG GCAGTCAACA 
GATCTTGAGT CCTTGCTGAA TGGGAATGGA GACCCTTTCC CACATTTCAA AGAGCTTCTT 
CAAAGCTACA GGGCTGAATG GGTGAAGGAT GAGTTCAAAT ACGGTCATTA TGAAGCTGTG 
CCACCGCCAG CCTTAGAACA TCAGATTTTT GAAGGACCCG ACACTGACAT ACAGACAGAG 
TTTCGGCTTT CTAACGTCAG ACTCGGCAGA AATGGTGATG TTACTGATGA TGAAGATCCT 
CAAACTCCAC GACGGCTTTC AAGACGTTTT TCTGTACGGT CATCTGGTCG TTTCTCTATG 
AGATCTACGA CTAGTGATGC ACCTGAAGCA TTGCACACAA ATATCTCAAA GCATTATGGG 
GTTGCTCCGT TACCTCCTTA TGAGCCCGTT TATAATTGGC GAGCAACAAG GGCATCTGTA 
CTTGGACAAA GAACTTCGGA ACTTCCATCA GCAATATCAT CTAGTAGCGG GCTGAAGGTT 
TCTGTGAAGG TTGTCTCTTT GAATGTTCAG GCCGGACTTG TCGAACCCAT GTACGGTACC 
TTGTGTCTCT ATCATCGAGA GAAGCGGGAG AAATTATCAG AAAATTTTCA CTTCCGATGT 
TTACCATCAG AGTTCCAAGA TTGTGTAACT TGGCAGGAGG GTGGTAATTG TCTGAGAAGA 
GCTATATTTT CACTGGAGGC AGCGTCGCCA GCAATATGTT TGTTGATCCA GCTTGAAAAG 
CATGTGACTG AGGAAGGAGG AGTAACGGCT CATGTTTATT CTCGAAAAGA ACCTGTACAC 
TTGACCGAGA GAGAAAAGCA GAAGTTACAG GTCTGGGCGC GCGTGATGCC ATTCAGGGAG 
CCTTTCGCGT GGGCAGTAGT TGCTCTGTTT GATGCAAATA TAACAGGAGG TGTAGGAGGA 
TTCTCATCTT CTCCAAGCAG CCCACTTCCA CCAGGACTTT TTGGACCATC TTTGATGGAA 
GCATCCTTGG ATTCTGATGG ACGACTTATA GCTGATGCTA AGACAGAATC ATCTAGTGTG 
CCAGTTTTGG TTGATGTTCA GAGCCTTCGC CGGGTCAAAG AAAATTACAC GGAGGAAATG 
CTTCAGGATC CCAAGTACAA AGTGCACAAA CCCGTGAAGG CCATCCTTCG TCTCGAGATA 
GAGAGGTTAT CTACTGAAGA TCATGACCAA GATTCCATAT TTGAGTGTGG TAGTTTTGGT 
CGTGGGTCCA CTGATGGTGA TTCCCGCTTG ACAGTAGGAC CCTCTTTCTC AGGGCGTTTC 
ATGCAGGGGC TGGGCATGAC GAAGGTTACG CATAATGGGC GGCAGAAATG GAGCCCTGGA 
AGAAAACATC GCTATCTGCA TTCCGGCAGT GTTGGCAGCA TTGAATTTGG AAAGTTTGAC 
TTCCGGGCTA TTGAGTTTCG ATCGGGTTCG AAAGTTGAGG TTTTACCTCA GCTATTGCAC 
TGCCTGTATG TATATCCTCA ATCAGTCAAT TTAAGTCGAA AACGCAATCT TTTTGTGCGA 
GTGGAGCTTC GAAAGGATGA TGTAGACATT CGCAAATCTC CTCTCGAGGC CCTTTATCCG 
AGGGATGCTG ACAGCTCTAT GCAGAAATAT GCACACTCTC AGATCGATGC AAACACCAAG 
ACACCTCACT ATCATGATGA GTTCAAAATT CAACTACCTG CCGTTCTCTC TCCTGAGCAT 
CACCTTCTGT TCACGTTTTT TCATGTGGAT CTACAGATGA AAATTGAGGC TCCAAAGCCG 
GTGGTAGTGG GATACTCTGT TCTTCCGCTC ATGTTTGTTT GTCAGGTTCA GCGGTTGGAC 
GGCAGCCTAC CTATCGCAAA GGAGCTGTAT TCCAATTATC TACAAGAAAA TGTGAAGGAA 
AGGTTGGAGT ACTTGGATGA TGGCAAAACT GTATTTAAAT TGAGGTCACG ATTATGCTCT 
TCTCTTTATC CGGTTAATGA GAGGATCCGA GATTTCTTTT CAGAATACGA TCGACATGTC 
TTGCGGACCA GTCCACCCTG GGGTACTGAG CTGATGGAGG CTATTAATGC TCTGAAGACG 
GTTGAGCCGT CAGCTATGCT CCAGTTTCTT CAACCTATTC TCAACATGCT GCTTCGTATG 
ATTCGCGATG GAGGAGAAAC GTTGCAGGTT GCTGCATTCC GTTCAATGGT TAACATCATT 
ACCAGGGTAC AAGCAGAGAC ATCTGATGGG GCTGAAAGAA ATCGTTACCT TGTGCATTAT 
GTGGATTATG CATTTAATGA TTTTGGTGGG CGTCATGAGC CTGTGTACCC AGGTCTGTGC 
AGTGTATGGC GCAGCCTTGC TCGAAGCAAG GCGAAAGGCT ACCGTGTCGG ACCTGTGTAT 
GATGATGTTC TTTCCATGGC TTGGGTGTTC CTGGAGCTAA TTGTCAAGTC CATGGCACTT 
GAACAATCCC GCATTTACTC TGAGTCCCTC CCACCTGGAG AGGAGTTACC ACCTTTGCAG 
CTCATTGATG AGGTCTTTAA AAGTATCGTA CAGCTCTATG ATTGTTTACT GACAGAGGTT 
CATGAGCGCT GCAAGAAGGG CATGCTTCTC GCAATGAAGC TGAATAGCAG TATTGCATAC 
TTTTGCTATG ACCTTCTCTC TGTAATTGAG CATCGACAAG TCTTTGAGCT GGTTGCGCTT 
TATTTCAACA AGTTTGCTGG GGTATGTCAA TCTCTTGTGC ACGAATGTAA GCTTAATTTT 
CTGCGCATCG TCTGTGACCA TGATCTCTTT GTTGAGATGC CCGGTCGAGA TCCTACAGAG 
AGGAATTATT TGGCATCACT GTTGATGCAA GAGCTCTTTG TTACTTGGGA TCATGAAGAT 
CTGACTTTAA GATCTAAGGC AGCTCGAATC CTTGTGCAAC TTATGTGCAA GCATGAGTAT 
GATGCACGGT ATCAAACATT GGAGGACAAA ATGTATATTG CCCAACGTTA CTTTCCACTA 
GTTGACCTGA TATTGGACGA AATGCCAGTG TTCTATGGCC TGAGCACTAC AGAGAAGCGT 
GAAGTGCTGG TGTGTGTGCT TCACATTCTG CGATATCTAG ACGATGCATC TCTTGTTAAA 
GCTTGGCAGC ACAACATGGA TAGAACAAAG CTTTTTTTCA AACTCCTGGA AGAATGTCAG 
GAATTGCTGG AGTATAAGAA GGCTGGAGCA GATGGCTTGA TGGGAGCTAT GCCAGCGAAT 
GATCAAGGAG AAGGGCCCCT TCGATACTCC GAGAAGCTCT CACCTGCAGT CAATCAGTTT 
TTGATTGAGG CATCTCGGCA GGATGTCAGA CTCACAAAGA TTTCTCCAAA TGCTCAGTGG 
ATACTTCCAC AAACGAGTCC AGAGATTGGT TCATTCTGGA AAAGAGCAAG CCCTGTAACA 
AATAGTTCAA CTAGCCCAGG TCAGCCGCAT TCGTTGCGAG AGGCACTAGC CCAAGCACAG 
TCTGCGGGAA GAAGTGGAGG CAGGTCTCTT CGTGAGACCT TGCATCCTAT GCTCCGCCAA 
AAGTTGGAGC TATGGGAGGA GAACCTTAGT GCCTCTGCTA TGCTCCAAAT TTTGGAGGTA 
CTTGAGAAGT TTATAAGTGC AGTATCAAGT CAAGCTGTTC TCACTGACTA CATGAGGCTT 
GATTGTATCA CAACTATTTT TATTGGTTTT CTGGGACGCA GCCAGCCGTT GCCATTTTGG 
AAGGCCTTTC TACCTTTCTT CAATAACGTG TTTCGTCGTC ATGGCGCTAC CTTGATGAGT 
AGGGAGAATG ATAGGTTCTT GAAGCAAGTA GCATTTCATC TACTACGGCT CGGAGTTTTC 
CGGAATGAGT CCATACGGAA ACGTTCTGTT GTTGGCCTTC AAATTCTTGT CCGAAATGCA 
TACCACTACT TTCAAGGCCT TTCAAGACTG CGGGTGATGC TTACAATCAC ACTTTCTGAG 
CTCATGTCAG ATGTTCAAGT AACACATCCT AAGATGGATG GATCCTTGGA AGAGAGTGGT 
GAATCTCAAC GTCTTCGTAG GTCCTTGCAA CAGATTGCAC AAGAAAGTAT TAGTGCGGAT 
CTTCTACGGG AATGTGGTCT TCCTGAAGAT TCTCTTACTG CTTTGCCAGA AGGTGTGAAT 
GGCCAACAAT GGAGTTGGGC TGGAGTCGCT GAGCTTTCCA CCTCTCTGCT GAAAGCTGTG 
GATGCTGCTG TAGGACATGC TCTTTTGGGT CCTGAGATTT TAATGAATGA CAAGTATGCA 
ACTGCAGAGG CCTACCATGG TTTGGCGACG GCATATACCA ACGTGCCCGA TCTGCATATA 
ATGTGGCTTC TGTATCTCTG CGAAGTTCAT CAAGGCAACC AGTCTTATGC AGAGGCTGCT 
CAGTGTGCTG TTGCGGTTGC AGGTGTCATC ATGCATGCTA TAGTAGGAAA AGGTGATCAT 
GTTTGGGGAA AAGAGCATGT TGAGGCACTG GGTAATATTT TCCCAATGCT CACTGGTTCC 
CGCTTTGGGG ATGCTGCAAC TGCCGAAATT GAAGGCTATG GGTCATCGAA ATTGACTGTC 
GAATCGGCAG TGAAGTACTT GCAGCTCGCC AATAAACTCT TTGTCCAGGC AGAGCTCTTC 
CATTTCTGTG CCGGCATATT GGAGCTCATA ATTCCCGTCC ATAAGTCAAG GCGTGCCTAT 
AGTCAGCTGT CTAAATGTCA TACTTCATTG ACCAGCATCT ACGAAGCTAT ACTAGAGCAA 
GAGTCGAGCC CTATCCCTTT CACGGATGCA ACATATTATC GTGTGGGATT TTATGGGAAA 
AGCTTTGGTA GTTTGCACTG CAAGGAGTAT GTGTACAGGG AACCACGGGA CGTGCGGTTA 
GGGGACATTA TGAAAAATTT AGGAAATATT TATGAGCCTA CAGTAATTGA AGGAAAGCAG 
AACTTGCATA TTATCCCCGA TTCTCGTCAA GTGAATCCAG AGGATCTTCA AGCTGGCATT 
TGTTACTTGC AAATTACATC TGTCGATCCT GTTACAGAGG ACGAGGATTT GGAAAGCAGG 
CGAGAAAGAC AATCCGGGAG GTCCTCGTCA ACATTCAGTG CACGAGTCTT CGATCGATTT 
TTATTTGACA CTCCTTTCAC AAAGAATGGA AGATCCCAAG GAGGTTTGGA AGATCAGTGG 
AAGAGGCGTA CGGTTTTGTG GACTGAAGGG CCTTTTCCAT CTCTTGTAAA TCGTTTAATG 
GTTGTGAAGT CTGAGTCGCG AGAATTTTCG CCAATCGAAA ACGCCATAGG AATGATTGAG 
ACTCGTACAG CAGCTTTAGC AGGAGAGCTC GATGATCAAC GTATAAATGA GGGAGATCCT 
CTGCCCCGTT TACAAGGCCT GCAAAGAATC TTGCAAGGAA GTGTTGCAGT CCAGGTAAAT 
AGTGGAGTGC TAGGTGTCTG CACAGCTTTC TTGTCTGGGG AACCTTCAAC ACGATTGCGC 
TCACAAGAAC TACAGCAGCT GATAGCAGCA CTCTTGGAGT TTATGGCTGT GTGCAAGAAA 
GCAATTCGAG TACATGCACG ACTGATTGGA GAGGAGGACC AAGACTTCCA CTCGCAGCTT 
GTGATTGGAT TCCAATCACT CACAGCAGAG CTATCCCACT ATATTCCCGC CATCTTGTCA 
GATGTAACCA TACTTGGGAA ACACAGCAAT GTGCTCAAGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024369311.1
CDS	418..6342
Translation	MHLVAGAPIWSVESVKEGKGSRFDVSDSVGQCRVGSHDRRDEEETWGLNAETESSAFWELSFETRAVVDSGSIKKQGMDEIALSGQRFKRIPHPYIGQSTDLESLLNGNGDPFPHFKELLQSYRAEWVKDEFKYGHYEAVPPPALEHQIFEGPDTDIQTEFRLSNVRLGRNGDVTDDEDPQTPRRLSRRFSVRSSGRFSMRSTTSDAPEALHTNISKHYGVAPLPPYEPVYNWRATRASVLGQRTSELPSAISSSSGLKVSVKVVSLNVQAGLVEPMYGTLCLYHREKREKLSENFHFRCLPSEFQDCVTWQEGGNCLRRAIFSLEAASPAICLLIQLEKHVTEEGGVTAHVYSRKEPVHLTEREKQKLQVWARVMPFREPFAWAVVALFDANITGGVGGFSSSPSSPLPPGLFGPSLMEASLDSDGRLIADAKTESSSVPVLVDVQSLRRVKENYTEEMLQDPKYKVHKPVKAILRLEIERLSTEDHDQDSIFECGSFGRGSTDGDSRLTVGPSFSGRFMQGLGMTKVTHNGRQKWSPGRKHRYLHSGSVGSIEFGKFDFRAIEFRSGSKVEVLPQLLHCLYVYPQSVNLSRKRNLFVRVELRKDDVDIRKSPLEALYPRDADSSMQKYAHSQIDANTKTPHYHDEFKIQLPAVLSPEHHLLFTFFHVDLQMKIEAPKPVVVGYSVLPLMFVCQVQRLDGSLPIAKELYSNYLQENVKERLEYLDDGKTVFKLRSRLCSSLYPVNERIRDFFSEYDRHVLRTSPPWGTELMEAINALKTVEPSAMLQFLQPILNMLLRMIRDGGETLQVAAFRSMVNIITRVQAETSDGAERNRYLVHYVDYAFNDFGGRHEPVYPGLCSVWRSLARSKAKGYRVGPVYDDVLSMAWVFLELIVKSMALEQSRIYSESLPPGEELPPLQLIDEVFKSIVQLYDCLLTEVHERCKKGMLLAMKLNSSIAYFCYDLLSVIEHRQVFELVALYFNKFAGVCQSLVHECKLNFLRIVCDHDLFVEMPGRDPTERNYLASLLMQELFVTWDHEDLTLRSKAARILVQLMCKHEYDARYQTLEDKMYIAQRYFPLVDLILDEMPVFYGLSTTEKREVLVCVLHILRYLDDASLVKAWQHNMDRTKLFFKLLEECQELLEYKKAGADGLMGAMPANDQGEGPLRYSEKLSPAVNQFLIEASRQDVRLTKISPNAQWILPQTSPEIGSFWKRASPVTNSSTSPGQPHSLREALAQAQSAGRSGGRSLRETLHPMLRQKLELWEENLSASAMLQILEVLEKFISAVSSQAVLTDYMRLDCITTIFIGFLGRSQPLPFWKAFLPFFNNVFRRHGATLMSRENDRFLKQVAFHLLRLGVFRNESIRKRSVVGLQILVRNAYHYFQGLSRLRVMLTITLSELMSDVQVTHPKMDGSLEESGESQRLRRSLQQIAQESISADLLRECGLPEDSLTALPEGVNGQQWSWAGVAELSTSLLKAVDAAVGHALLGPEILMNDKYATAEAYHGLATAYTNVPDLHIMWLLYLCEVHQGNQSYAEAAQCAVAVAGVIMHAIVGKGDHVWGKEHVEALGNIFPMLTGSRFGDAATAEIEGYGSSKLTVESAVKYLQLANKLFVQAELFHFCAGILELIIPVHKSRRAYSQLSKCHTSLTSIYEAILEQESSPIPFTDATYYRVGFYGKSFGSLHCKEYVYREPRDVRLGDIMKNLGNIYEPTVIEGKQNLHIIPDSRQVNPEDLQAGICYLQITSVDPVTEDEDLESRRERQSGRSSSTFSARVFDRFLFDTPFTKNGRSQGGLEDQWKRRTVLWTEGPFPSLVNRLMVVKSESREFSPIENAIGMIETRTAALAGELDDQRINEGDPLPRLQGLQRILQGSVAVQVNSGVLGVCTAFLSGEPSTRLRSQELQQLIAALLEFMAVCKKAIRVHARLIGEEDQDFHSQLVIGFQSLTAELSHYIPAILSDVTILGKHSNVLKL

Target ORF information:

RefSeq Version	XM_024513543.1
Organism	Physcomitrella patens
Definition	Physcomitrella patens guanine nucleotide exchange factor SPIKE 1-like (LOC112279271), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024513543.1

ORF Insert Sequence:

ATGCATTTGG TCGCTGGAGC GCCTATATGG TCGGTGGAAA GCGTCAAGGA GGGAAAAGGA 
TCCAGGTTCG ACGTGTCGGA CTCAGTAGGA CAATGTAGGG TAGGTAGTCA TGATCGGAGG 
GACGAGGAGG AGACATGGGG ACTCAATGCA GAGACAGAAA GCTCTGCATT CTGGGAGCTG 
AGCTTCGAGA CTAGAGCTGT CGTCGACAGC GGATCAATTA AGAAGCAGGG GATGGATGAA 
ATTGCCTTGA GCGGGCAGCG GTTCAAACGT ATCCCACACC CGTACATCGG GCAGTCAACA 
GATCTTGAGT CCTTGCTGAA TGGGAATGGA GACCCTTTCC CACATTTCAA AGAGCTTCTT 
CAAAGCTACA GGGCTGAATG GGTGAAGGAT GAGTTCAAAT ACGGTCATTA TGAAGCTGTG 
CCACCGCCAG CCTTAGAACA TCAGATTTTT GAAGGACCCG ACACTGACAT ACAGACAGAG 
TTTCGGCTTT CTAACGTCAG ACTCGGCAGA AATGGTGATG TTACTGATGA TGAAGATCCT 
CAAACTCCAC GACGGCTTTC AAGACGTTTT TCTGTACGGT CATCTGGTCG TTTCTCTATG 
AGATCTACGA CTAGTGATGC ACCTGAAGCA TTGCACACAA ATATCTCAAA GCATTATGGG 
GTTGCTCCGT TACCTCCTTA TGAGCCCGTT TATAATTGGC GAGCAACAAG GGCATCTGTA 
CTTGGACAAA GAACTTCGGA ACTTCCATCA GCAATATCAT CTAGTAGCGG GCTGAAGGTT 
TCTGTGAAGG TTGTCTCTTT GAATGTTCAG GCCGGACTTG TCGAACCCAT GTACGGTACC 
TTGTGTCTCT ATCATCGAGA GAAGCGGGAG AAATTATCAG AAAATTTTCA CTTCCGATGT 
TTACCATCAG AGTTCCAAGA TTGTGTAACT TGGCAGGAGG GTGGTAATTG TCTGAGAAGA 
GCTATATTTT CACTGGAGGC AGCGTCGCCA GCAATATGTT TGTTGATCCA GCTTGAAAAG 
CATGTGACTG AGGAAGGAGG AGTAACGGCT CATGTTTATT CTCGAAAAGA ACCTGTACAC 
TTGACCGAGA GAGAAAAGCA GAAGTTACAG GTCTGGGCGC GCGTGATGCC ATTCAGGGAG 
CCTTTCGCGT GGGCAGTAGT TGCTCTGTTT GATGCAAATA TAACAGGAGG TGTAGGAGGA 
TTCTCATCTT CTCCAAGCAG CCCACTTCCA CCAGGACTTT TTGGACCATC TTTGATGGAA 
GCATCCTTGG ATTCTGATGG ACGACTTATA GCTGATGCTA AGACAGAATC ATCTAGTGTG 
CCAGTTTTGG TTGATGTTCA GAGCCTTCGC CGGGTCAAAG AAAATTACAC GGAGGAAATG 
CTTCAGGATC CCAAGTACAA AGTGCACAAA CCCGTGAAGG CCATCCTTCG TCTCGAGATA 
GAGAGGTTAT CTACTGAAGA TCATGACCAA GATTCCATAT TTGAGTGTGG TAGTTTTGGT 
CGTGGGTCCA CTGATGGTGA TTCCCGCTTG ACAGTAGGAC CCTCTTTCTC AGGGCGTTTC 
ATGCAGGGGC TGGGCATGAC GAAGGTTACG CATAATGGGC GGCAGAAATG GAGCCCTGGA 
AGAAAACATC GCTATCTGCA TTCCGGCAGT GTTGGCAGCA TTGAATTTGG AAAGTTTGAC 
TTCCGGGCTA TTGAGTTTCG ATCGGGTTCG AAAGTTGAGG TTTTACCTCA GCTATTGCAC 
TGCCTGTATG TATATCCTCA ATCAGTCAAT TTAAGTCGAA AACGCAATCT TTTTGTGCGA 
GTGGAGCTTC GAAAGGATGA TGTAGACATT CGCAAATCTC CTCTCGAGGC CCTTTATCCG 
AGGGATGCTG ACAGCTCTAT GCAGAAATAT GCACACTCTC AGATCGATGC AAACACCAAG 
ACACCTCACT ATCATGATGA GTTCAAAATT CAACTACCTG CCGTTCTCTC TCCTGAGCAT 
CACCTTCTGT TCACGTTTTT TCATGTGGAT CTACAGATGA AAATTGAGGC TCCAAAGCCG 
GTGGTAGTGG GATACTCTGT TCTTCCGCTC ATGTTTGTTT GTCAGGTTCA GCGGTTGGAC 
GGCAGCCTAC CTATCGCAAA GGAGCTGTAT TCCAATTATC TACAAGAAAA TGTGAAGGAA 
AGGTTGGAGT ACTTGGATGA TGGCAAAACT GTATTTAAAT TGAGGTCACG ATTATGCTCT 
TCTCTTTATC CGGTTAATGA GAGGATCCGA GATTTCTTTT CAGAATACGA TCGACATGTC 
TTGCGGACCA GTCCACCCTG GGGTACTGAG CTGATGGAGG CTATTAATGC TCTGAAGACG 
GTTGAGCCGT CAGCTATGCT CCAGTTTCTT CAACCTATTC TCAACATGCT GCTTCGTATG 
ATTCGCGATG GAGGAGAAAC GTTGCAGGTT GCTGCATTCC GTTCAATGGT TAACATCATT 
ACCAGGGTAC AAGCAGAGAC ATCTGATGGG GCTGAAAGAA ATCGTTACCT TGTGCATTAT 
GTGGATTATG CATTTAATGA TTTTGGTGGG CGTCATGAGC CTGTGTACCC AGGTCTGTGC 
AGTGTATGGC GCAGCCTTGC TCGAAGCAAG GCGAAAGGCT ACCGTGTCGG ACCTGTGTAT 
GATGATGTTC TTTCCATGGC TTGGGTGTTC CTGGAGCTAA TTGTCAAGTC CATGGCACTT 
GAACAATCCC GCATTTACTC TGAGTCCCTC CCACCTGGAG AGGAGTTACC ACCTTTGCAG 
CTCATTGATG AGGTCTTTAA AAGTATCGTA CAGCTCTATG ATTGTTTACT GACAGAGGTT 
CATGAGCGCT GCAAGAAGGG CATGCTTCTC GCAATGAAGC TGAATAGCAG TATTGCATAC 
TTTTGCTATG ACCTTCTCTC TGTAATTGAG CATCGACAAG TCTTTGAGCT GGTTGCGCTT 
TATTTCAACA AGTTTGCTGG GGTATGTCAA TCTCTTGTGC ACGAATGTAA GCTTAATTTT 
CTGCGCATCG TCTGTGACCA TGATCTCTTT GTTGAGATGC CCGGTCGAGA TCCTACAGAG 
AGGAATTATT TGGCATCACT GTTGATGCAA GAGCTCTTTG TTACTTGGGA TCATGAAGAT 
CTGACTTTAA GATCTAAGGC AGCTCGAATC CTTGTGCAAC TTATGTGCAA GCATGAGTAT 
GATGCACGGT ATCAAACATT GGAGGACAAA ATGTATATTG CCCAACGTTA CTTTCCACTA 
GTTGACCTGA TATTGGACGA AATGCCAGTG TTCTATGGCC TGAGCACTAC AGAGAAGCGT 
GAAGTGCTGG TGTGTGTGCT TCACATTCTG CGATATCTAG ACGATGCATC TCTTGTTAAA 
GCTTGGCAGC ACAACATGGA TAGAACAAAG CTTTTTTTCA AACTCCTGGA AGAATGTCAG 
GAATTGCTGG AGTATAAGAA GGCTGGAGCA GATGGCTTGA TGGGAGCTAT GCCAGCGAAT 
GATCAAGGAG AAGGGCCCCT TCGATACTCC GAGAAGCTCT CACCTGCAGT CAATCAGTTT 
TTGATTGAGG CATCTCGGCA GGATGTCAGA CTCACAAAGA TTTCTCCAAA TGCTCAGTGG 
ATACTTCCAC AAACGAGTCC AGAGATTGGT TCATTCTGGA AAAGAGCAAG CCCTGTAACA 
AATAGTTCAA CTAGCCCAGG TCAGCCGCAT TCGTTGCGAG AGGCACTAGC CCAAGCACAG 
TCTGCGGGAA GAAGTGGAGG CAGGTCTCTT CGTGAGACCT TGCATCCTAT GCTCCGCCAA 
AAGTTGGAGC TATGGGAGGA GAACCTTAGT GCCTCTGCTA TGCTCCAAAT TTTGGAGGTA 
CTTGAGAAGT TTATAAGTGC AGTATCAAGT CAAGCTGTTC TCACTGACTA CATGAGGCTT 
GATTGTATCA CAACTATTTT TATTGGTTTT CTGGGACGCA GCCAGCCGTT GCCATTTTGG 
AAGGCCTTTC TACCTTTCTT CAATAACGTG TTTCGTCGTC ATGGCGCTAC CTTGATGAGT 
AGGGAGAATG ATAGGTTCTT GAAGCAAGTA GCATTTCATC TACTACGGCT CGGAGTTTTC 
CGGAATGAGT CCATACGGAA ACGTTCTGTT GTTGGCCTTC AAATTCTTGT CCGAAATGCA 
TACCACTACT TTCAAGGCCT TTCAAGACTG CGGGTGATGC TTACAATCAC ACTTTCTGAG 
CTCATGTCAG ATGTTCAAGT AACACATCCT AAGATGGATG GATCCTTGGA AGAGAGTGGT 
GAATCTCAAC GTCTTCGTAG GTCCTTGCAA CAGATTGCAC AAGAAAGTAT TAGTGCGGAT 
CTTCTACGGG AATGTGGTCT TCCTGAAGAT TCTCTTACTG CTTTGCCAGA AGGTGTGAAT 
GGCCAACAAT GGAGTTGGGC TGGAGTCGCT GAGCTTTCCA CCTCTCTGCT GAAAGCTGTG 
GATGCTGCTG TAGGACATGC TCTTTTGGGT CCTGAGATTT TAATGAATGA CAAGTATGCA 
ACTGCAGAGG CCTACCATGG TTTGGCGACG GCATATACCA ACGTGCCCGA TCTGCATATA 
ATGTGGCTTC TGTATCTCTG CGAAGTTCAT CAAGGCAACC AGTCTTATGC AGAGGCTGCT 
CAGTGTGCTG TTGCGGTTGC AGGTGTCATC ATGCATGCTA TAGTAGGAAA AGGTGATCAT 
GTTTGGGGAA AAGAGCATGT TGAGGCACTG GGTAATATTT TCCCAATGCT CACTGGTTCC 
CGCTTTGGGG ATGCTGCAAC TGCCGAAATT GAAGGCTATG GGTCATCGAA ATTGACTGTC 
GAATCGGCAG TGAAGTACTT GCAGCTCGCC AATAAACTCT TTGTCCAGGC AGAGCTCTTC 
CATTTCTGTG CCGGCATATT GGAGCTCATA ATTCCCGTCC ATAAGTCAAG GCGTGCCTAT 
AGTCAGCTGT CTAAATGTCA TACTTCATTG ACCAGCATCT ACGAAGCTAT ACTAGAGCAA 
GAGTCGAGCC CTATCCCTTT CACGGATGCA ACATATTATC GTGTGGGATT TTATGGGAAA 
AGCTTTGGTA GTTTGCACTG CAAGGAGTAT GTGTACAGGG AACCACGGGA CGTGCGGTTA 
GGGGACATTA TGAAAAATTT AGGAAATATT TATGAGCCTA CAGTAATTGA AGGAAAGCAG 
AACTTGCATA TTATCCCCGA TTCTCGTCAA GTGAATCCAG AGGATCTTCA AGCTGGCATT 
TGTTACTTGC AAATTACATC TGTCGATCCT GTTACAGAGG ACGAGGATTT GGAAAGCAGG 
CGAGAAAGAC AATCCGGGAG GTCCTCGTCA ACATTCAGTG CACGAGTCTT CGATCGATTT 
TTATTTGACA CTCCTTTCAC AAAGAATGGA AGATCCCAAG GAGGTTTGGA AGATCAGTGG 
AAGAGGCGTA CGGTTTTGTG GACTGAAGGG CCTTTTCCAT CTCTTGTAAA TCGTTTAATG 
GTTGTGAAGT CTGAGTCGCG AGAATTTTCG CCAATCGAAA ACGCCATAGG AATGATTGAG 
ACTCGTACAG CAGCTTTAGC AGGAGAGCTC GATGATCAAC GTATAAATGA GGGAGATCCT 
CTGCCCCGTT TACAAGGCCT GCAAAGAATC TTGCAAGGAA GTGTTGCAGT CCAGGTAAAT 
AGTGGAGTGC TAGGTGTCTG CACAGCTTTC TTGTCTGGGG AACCTTCAAC ACGATTGCGC 
TCACAAGAAC TACAGCAGCT GATAGCAGCA CTCTTGGAGT TTATGGCTGT GTGCAAGAAA 
GCAATTCGAG TACATGCACG ACTGATTGGA GAGGAGGACC AAGACTTCCA CTCGCAGCTT 
GTGATTGGAT TCCAATCACT CACAGCAGAG CTATCCCACT ATATTCCCGC CATCTTGTCA 
GATGTAACCA TACTTGGGAA ACACAGCAAT GTGCTCAAGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC112279271 ( XM_024513534.1 ) cDNA ORF clone, -> XP_024369302.1 Physcomitrella patens guanine nucleotide exchange factor SPIKE 1-like (LOC112279271), transcript variant X1, mRNA.

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CloneID	OPn41280
Clone ID Related Accession (Same CDS sequence)	XM_024513560.1 , XM_024513543.1 , XM_024513534.1 , XM_024513569.1 , XM_024513576.1 , XM_024513552.1 , XM_024513585.1
Accession Version	XM_024513534.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Physcomitrella patens
Product	guanine nucleotide exchange factor SPIKE 1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037253.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physcomitrella patens Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATTTGG TCGCTGGAGC GCCTATATGG TCGGTGGAAA GCGTCAAGGA GGGAAAAGGA 
TCCAGGTTCG ACGTGTCGGA CTCAGTAGGA CAATGTAGGG TAGGTAGTCA TGATCGGAGG 
GACGAGGAGG AGACATGGGG ACTCAATGCA GAGACAGAAA GCTCTGCATT CTGGGAGCTG 
AGCTTCGAGA CTAGAGCTGT CGTCGACAGC GGATCAATTA AGAAGCAGGG GATGGATGAA 
ATTGCCTTGA GCGGGCAGCG GTTCAAACGT ATCCCACACC CGTACATCGG GCAGTCAACA 
GATCTTGAGT CCTTGCTGAA TGGGAATGGA GACCCTTTCC CACATTTCAA AGAGCTTCTT 
CAAAGCTACA GGGCTGAATG GGTGAAGGAT GAGTTCAAAT ACGGTCATTA TGAAGCTGTG 
CCACCGCCAG CCTTAGAACA TCAGATTTTT GAAGGACCCG ACACTGACAT ACAGACAGAG 
TTTCGGCTTT CTAACGTCAG ACTCGGCAGA AATGGTGATG TTACTGATGA TGAAGATCCT 
CAAACTCCAC GACGGCTTTC AAGACGTTTT TCTGTACGGT CATCTGGTCG TTTCTCTATG 
AGATCTACGA CTAGTGATGC ACCTGAAGCA TTGCACACAA ATATCTCAAA GCATTATGGG 
GTTGCTCCGT TACCTCCTTA TGAGCCCGTT TATAATTGGC GAGCAACAAG GGCATCTGTA 
CTTGGACAAA GAACTTCGGA ACTTCCATCA GCAATATCAT CTAGTAGCGG GCTGAAGGTT 
TCTGTGAAGG TTGTCTCTTT GAATGTTCAG GCCGGACTTG TCGAACCCAT GTACGGTACC 
TTGTGTCTCT ATCATCGAGA GAAGCGGGAG AAATTATCAG AAAATTTTCA CTTCCGATGT 
TTACCATCAG AGTTCCAAGA TTGTGTAACT TGGCAGGAGG GTGGTAATTG TCTGAGAAGA 
GCTATATTTT CACTGGAGGC AGCGTCGCCA GCAATATGTT TGTTGATCCA GCTTGAAAAG 
CATGTGACTG AGGAAGGAGG AGTAACGGCT CATGTTTATT CTCGAAAAGA ACCTGTACAC 
TTGACCGAGA GAGAAAAGCA GAAGTTACAG GTCTGGGCGC GCGTGATGCC ATTCAGGGAG 
CCTTTCGCGT GGGCAGTAGT TGCTCTGTTT GATGCAAATA TAACAGGAGG TGTAGGAGGA 
TTCTCATCTT CTCCAAGCAG CCCACTTCCA CCAGGACTTT TTGGACCATC TTTGATGGAA 
GCATCCTTGG ATTCTGATGG ACGACTTATA GCTGATGCTA AGACAGAATC ATCTAGTGTG 
CCAGTTTTGG TTGATGTTCA GAGCCTTCGC CGGGTCAAAG AAAATTACAC GGAGGAAATG 
CTTCAGGATC CCAAGTACAA AGTGCACAAA CCCGTGAAGG CCATCCTTCG TCTCGAGATA 
GAGAGGTTAT CTACTGAAGA TCATGACCAA GATTCCATAT TTGAGTGTGG TAGTTTTGGT 
CGTGGGTCCA CTGATGGTGA TTCCCGCTTG ACAGTAGGAC CCTCTTTCTC AGGGCGTTTC 
ATGCAGGGGC TGGGCATGAC GAAGGTTACG CATAATGGGC GGCAGAAATG GAGCCCTGGA 
AGAAAACATC GCTATCTGCA TTCCGGCAGT GTTGGCAGCA TTGAATTTGG AAAGTTTGAC 
TTCCGGGCTA TTGAGTTTCG ATCGGGTTCG AAAGTTGAGG TTTTACCTCA GCTATTGCAC 
TGCCTGTATG TATATCCTCA ATCAGTCAAT TTAAGTCGAA AACGCAATCT TTTTGTGCGA 
GTGGAGCTTC GAAAGGATGA TGTAGACATT CGCAAATCTC CTCTCGAGGC CCTTTATCCG 
AGGGATGCTG ACAGCTCTAT GCAGAAATAT GCACACTCTC AGATCGATGC AAACACCAAG 
ACACCTCACT ATCATGATGA GTTCAAAATT CAACTACCTG CCGTTCTCTC TCCTGAGCAT 
CACCTTCTGT TCACGTTTTT TCATGTGGAT CTACAGATGA AAATTGAGGC TCCAAAGCCG 
GTGGTAGTGG GATACTCTGT TCTTCCGCTC ATGTTTGTTT GTCAGGTTCA GCGGTTGGAC 
GGCAGCCTAC CTATCGCAAA GGAGCTGTAT TCCAATTATC TACAAGAAAA TGTGAAGGAA 
AGGTTGGAGT ACTTGGATGA TGGCAAAACT GTATTTAAAT TGAGGTCACG ATTATGCTCT 
TCTCTTTATC CGGTTAATGA GAGGATCCGA GATTTCTTTT CAGAATACGA TCGACATGTC 
TTGCGGACCA GTCCACCCTG GGGTACTGAG CTGATGGAGG CTATTAATGC TCTGAAGACG 
GTTGAGCCGT CAGCTATGCT CCAGTTTCTT CAACCTATTC TCAACATGCT GCTTCGTATG 
ATTCGCGATG GAGGAGAAAC GTTGCAGGTT GCTGCATTCC GTTCAATGGT TAACATCATT 
ACCAGGGTAC AAGCAGAGAC ATCTGATGGG GCTGAAAGAA ATCGTTACCT TGTGCATTAT 
GTGGATTATG CATTTAATGA TTTTGGTGGG CGTCATGAGC CTGTGTACCC AGGTCTGTGC 
AGTGTATGGC GCAGCCTTGC TCGAAGCAAG GCGAAAGGCT ACCGTGTCGG ACCTGTGTAT 
GATGATGTTC TTTCCATGGC TTGGGTGTTC CTGGAGCTAA TTGTCAAGTC CATGGCACTT 
GAACAATCCC GCATTTACTC TGAGTCCCTC CCACCTGGAG AGGAGTTACC ACCTTTGCAG 
CTCATTGATG AGGTCTTTAA AAGTATCGTA CAGCTCTATG ATTGTTTACT GACAGAGGTT 
CATGAGCGCT GCAAGAAGGG CATGCTTCTC GCAATGAAGC TGAATAGCAG TATTGCATAC 
TTTTGCTATG ACCTTCTCTC TGTAATTGAG CATCGACAAG TCTTTGAGCT GGTTGCGCTT 
TATTTCAACA AGTTTGCTGG GGTATGTCAA TCTCTTGTGC ACGAATGTAA GCTTAATTTT 
CTGCGCATCG TCTGTGACCA TGATCTCTTT GTTGAGATGC CCGGTCGAGA TCCTACAGAG 
AGGAATTATT TGGCATCACT GTTGATGCAA GAGCTCTTTG TTACTTGGGA TCATGAAGAT 
CTGACTTTAA GATCTAAGGC AGCTCGAATC CTTGTGCAAC TTATGTGCAA GCATGAGTAT 
GATGCACGGT ATCAAACATT GGAGGACAAA ATGTATATTG CCCAACGTTA CTTTCCACTA 
GTTGACCTGA TATTGGACGA AATGCCAGTG TTCTATGGCC TGAGCACTAC AGAGAAGCGT 
GAAGTGCTGG TGTGTGTGCT TCACATTCTG CGATATCTAG ACGATGCATC TCTTGTTAAA 
GCTTGGCAGC ACAACATGGA TAGAACAAAG CTTTTTTTCA AACTCCTGGA AGAATGTCAG 
GAATTGCTGG AGTATAAGAA GGCTGGAGCA GATGGCTTGA TGGGAGCTAT GCCAGCGAAT 
GATCAAGGAG AAGGGCCCCT TCGATACTCC GAGAAGCTCT CACCTGCAGT CAATCAGTTT 
TTGATTGAGG CATCTCGGCA GGATGTCAGA CTCACAAAGA TTTCTCCAAA TGCTCAGTGG 
ATACTTCCAC AAACGAGTCC AGAGATTGGT TCATTCTGGA AAAGAGCAAG CCCTGTAACA 
AATAGTTCAA CTAGCCCAGG TCAGCCGCAT TCGTTGCGAG AGGCACTAGC CCAAGCACAG 
TCTGCGGGAA GAAGTGGAGG CAGGTCTCTT CGTGAGACCT TGCATCCTAT GCTCCGCCAA 
AAGTTGGAGC TATGGGAGGA GAACCTTAGT GCCTCTGCTA TGCTCCAAAT TTTGGAGGTA 
CTTGAGAAGT TTATAAGTGC AGTATCAAGT CAAGCTGTTC TCACTGACTA CATGAGGCTT 
GATTGTATCA CAACTATTTT TATTGGTTTT CTGGGACGCA GCCAGCCGTT GCCATTTTGG 
AAGGCCTTTC TACCTTTCTT CAATAACGTG TTTCGTCGTC ATGGCGCTAC CTTGATGAGT 
AGGGAGAATG ATAGGTTCTT GAAGCAAGTA GCATTTCATC TACTACGGCT CGGAGTTTTC 
CGGAATGAGT CCATACGGAA ACGTTCTGTT GTTGGCCTTC AAATTCTTGT CCGAAATGCA 
TACCACTACT TTCAAGGCCT TTCAAGACTG CGGGTGATGC TTACAATCAC ACTTTCTGAG 
CTCATGTCAG ATGTTCAAGT AACACATCCT AAGATGGATG GATCCTTGGA AGAGAGTGGT 
GAATCTCAAC GTCTTCGTAG GTCCTTGCAA CAGATTGCAC AAGAAAGTAT TAGTGCGGAT 
CTTCTACGGG AATGTGGTCT TCCTGAAGAT TCTCTTACTG CTTTGCCAGA AGGTGTGAAT 
GGCCAACAAT GGAGTTGGGC TGGAGTCGCT GAGCTTTCCA CCTCTCTGCT GAAAGCTGTG 
GATGCTGCTG TAGGACATGC TCTTTTGGGT CCTGAGATTT TAATGAATGA CAAGTATGCA 
ACTGCAGAGG CCTACCATGG TTTGGCGACG GCATATACCA ACGTGCCCGA TCTGCATATA 
ATGTGGCTTC TGTATCTCTG CGAAGTTCAT CAAGGCAACC AGTCTTATGC AGAGGCTGCT 
CAGTGTGCTG TTGCGGTTGC AGGTGTCATC ATGCATGCTA TAGTAGGAAA AGGTGATCAT 
GTTTGGGGAA AAGAGCATGT TGAGGCACTG GGTAATATTT TCCCAATGCT CACTGGTTCC 
CGCTTTGGGG ATGCTGCAAC TGCCGAAATT GAAGGCTATG GGTCATCGAA ATTGACTGTC 
GAATCGGCAG TGAAGTACTT GCAGCTCGCC AATAAACTCT TTGTCCAGGC AGAGCTCTTC 
CATTTCTGTG CCGGCATATT GGAGCTCATA ATTCCCGTCC ATAAGTCAAG GCGTGCCTAT 
AGTCAGCTGT CTAAATGTCA TACTTCATTG ACCAGCATCT ACGAAGCTAT ACTAGAGCAA 
GAGTCGAGCC CTATCCCTTT CACGGATGCA ACATATTATC GTGTGGGATT TTATGGGAAA 
AGCTTTGGTA GTTTGCACTG CAAGGAGTAT GTGTACAGGG AACCACGGGA CGTGCGGTTA 
GGGGACATTA TGAAAAATTT AGGAAATATT TATGAGCCTA CAGTAATTGA AGGAAAGCAG 
AACTTGCATA TTATCCCCGA TTCTCGTCAA GTGAATCCAG AGGATCTTCA AGCTGGCATT 
TGTTACTTGC AAATTACATC TGTCGATCCT GTTACAGAGG ACGAGGATTT GGAAAGCAGG 
CGAGAAAGAC AATCCGGGAG GTCCTCGTCA ACATTCAGTG CACGAGTCTT CGATCGATTT 
TTATTTGACA CTCCTTTCAC AAAGAATGGA AGATCCCAAG GAGGTTTGGA AGATCAGTGG 
AAGAGGCGTA CGGTTTTGTG GACTGAAGGG CCTTTTCCAT CTCTTGTAAA TCGTTTAATG 
GTTGTGAAGT CTGAGTCGCG AGAATTTTCG CCAATCGAAA ACGCCATAGG AATGATTGAG 
ACTCGTACAG CAGCTTTAGC AGGAGAGCTC GATGATCAAC GTATAAATGA GGGAGATCCT 
CTGCCCCGTT TACAAGGCCT GCAAAGAATC TTGCAAGGAA GTGTTGCAGT CCAGGTAAAT 
AGTGGAGTGC TAGGTGTCTG CACAGCTTTC TTGTCTGGGG AACCTTCAAC ACGATTGCGC 
TCACAAGAAC TACAGCAGCT GATAGCAGCA CTCTTGGAGT TTATGGCTGT GTGCAAGAAA 
GCAATTCGAG TACATGCACG ACTGATTGGA GAGGAGGACC AAGACTTCCA CTCGCAGCTT 
GTGATTGGAT TCCAATCACT CACAGCAGAG CTATCCCACT ATATTCCCGC CATCTTGTCA 
GATGTAACCA TACTTGGGAA ACACAGCAAT GTGCTCAAGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024369302.1
CDS	347..6271
Translation	MHLVAGAPIWSVESVKEGKGSRFDVSDSVGQCRVGSHDRRDEEETWGLNAETESSAFWELSFETRAVVDSGSIKKQGMDEIALSGQRFKRIPHPYIGQSTDLESLLNGNGDPFPHFKELLQSYRAEWVKDEFKYGHYEAVPPPALEHQIFEGPDTDIQTEFRLSNVRLGRNGDVTDDEDPQTPRRLSRRFSVRSSGRFSMRSTTSDAPEALHTNISKHYGVAPLPPYEPVYNWRATRASVLGQRTSELPSAISSSSGLKVSVKVVSLNVQAGLVEPMYGTLCLYHREKREKLSENFHFRCLPSEFQDCVTWQEGGNCLRRAIFSLEAASPAICLLIQLEKHVTEEGGVTAHVYSRKEPVHLTEREKQKLQVWARVMPFREPFAWAVVALFDANITGGVGGFSSSPSSPLPPGLFGPSLMEASLDSDGRLIADAKTESSSVPVLVDVQSLRRVKENYTEEMLQDPKYKVHKPVKAILRLEIERLSTEDHDQDSIFECGSFGRGSTDGDSRLTVGPSFSGRFMQGLGMTKVTHNGRQKWSPGRKHRYLHSGSVGSIEFGKFDFRAIEFRSGSKVEVLPQLLHCLYVYPQSVNLSRKRNLFVRVELRKDDVDIRKSPLEALYPRDADSSMQKYAHSQIDANTKTPHYHDEFKIQLPAVLSPEHHLLFTFFHVDLQMKIEAPKPVVVGYSVLPLMFVCQVQRLDGSLPIAKELYSNYLQENVKERLEYLDDGKTVFKLRSRLCSSLYPVNERIRDFFSEYDRHVLRTSPPWGTELMEAINALKTVEPSAMLQFLQPILNMLLRMIRDGGETLQVAAFRSMVNIITRVQAETSDGAERNRYLVHYVDYAFNDFGGRHEPVYPGLCSVWRSLARSKAKGYRVGPVYDDVLSMAWVFLELIVKSMALEQSRIYSESLPPGEELPPLQLIDEVFKSIVQLYDCLLTEVHERCKKGMLLAMKLNSSIAYFCYDLLSVIEHRQVFELVALYFNKFAGVCQSLVHECKLNFLRIVCDHDLFVEMPGRDPTERNYLASLLMQELFVTWDHEDLTLRSKAARILVQLMCKHEYDARYQTLEDKMYIAQRYFPLVDLILDEMPVFYGLSTTEKREVLVCVLHILRYLDDASLVKAWQHNMDRTKLFFKLLEECQELLEYKKAGADGLMGAMPANDQGEGPLRYSEKLSPAVNQFLIEASRQDVRLTKISPNAQWILPQTSPEIGSFWKRASPVTNSSTSPGQPHSLREALAQAQSAGRSGGRSLRETLHPMLRQKLELWEENLSASAMLQILEVLEKFISAVSSQAVLTDYMRLDCITTIFIGFLGRSQPLPFWKAFLPFFNNVFRRHGATLMSRENDRFLKQVAFHLLRLGVFRNESIRKRSVVGLQILVRNAYHYFQGLSRLRVMLTITLSELMSDVQVTHPKMDGSLEESGESQRLRRSLQQIAQESISADLLRECGLPEDSLTALPEGVNGQQWSWAGVAELSTSLLKAVDAAVGHALLGPEILMNDKYATAEAYHGLATAYTNVPDLHIMWLLYLCEVHQGNQSYAEAAQCAVAVAGVIMHAIVGKGDHVWGKEHVEALGNIFPMLTGSRFGDAATAEIEGYGSSKLTVESAVKYLQLANKLFVQAELFHFCAGILELIIPVHKSRRAYSQLSKCHTSLTSIYEAILEQESSPIPFTDATYYRVGFYGKSFGSLHCKEYVYREPRDVRLGDIMKNLGNIYEPTVIEGKQNLHIIPDSRQVNPEDLQAGICYLQITSVDPVTEDEDLESRRERQSGRSSSTFSARVFDRFLFDTPFTKNGRSQGGLEDQWKRRTVLWTEGPFPSLVNRLMVVKSESREFSPIENAIGMIETRTAALAGELDDQRINEGDPLPRLQGLQRILQGSVAVQVNSGVLGVCTAFLSGEPSTRLRSQELQQLIAALLEFMAVCKKAIRVHARLIGEEDQDFHSQLVIGFQSLTAELSHYIPAILSDVTILGKHSNVLKL

Target ORF information:

RefSeq Version	XM_024513534.1
Organism	Physcomitrella patens
Definition	Physcomitrella patens guanine nucleotide exchange factor SPIKE 1-like (LOC112279271), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024513534.1

ORF Insert Sequence:

ATGCATTTGG TCGCTGGAGC GCCTATATGG TCGGTGGAAA GCGTCAAGGA GGGAAAAGGA 
TCCAGGTTCG ACGTGTCGGA CTCAGTAGGA CAATGTAGGG TAGGTAGTCA TGATCGGAGG 
GACGAGGAGG AGACATGGGG ACTCAATGCA GAGACAGAAA GCTCTGCATT CTGGGAGCTG 
AGCTTCGAGA CTAGAGCTGT CGTCGACAGC GGATCAATTA AGAAGCAGGG GATGGATGAA 
ATTGCCTTGA GCGGGCAGCG GTTCAAACGT ATCCCACACC CGTACATCGG GCAGTCAACA 
GATCTTGAGT CCTTGCTGAA TGGGAATGGA GACCCTTTCC CACATTTCAA AGAGCTTCTT 
CAAAGCTACA GGGCTGAATG GGTGAAGGAT GAGTTCAAAT ACGGTCATTA TGAAGCTGTG 
CCACCGCCAG CCTTAGAACA TCAGATTTTT GAAGGACCCG ACACTGACAT ACAGACAGAG 
TTTCGGCTTT CTAACGTCAG ACTCGGCAGA AATGGTGATG TTACTGATGA TGAAGATCCT 
CAAACTCCAC GACGGCTTTC AAGACGTTTT TCTGTACGGT CATCTGGTCG TTTCTCTATG 
AGATCTACGA CTAGTGATGC ACCTGAAGCA TTGCACACAA ATATCTCAAA GCATTATGGG 
GTTGCTCCGT TACCTCCTTA TGAGCCCGTT TATAATTGGC GAGCAACAAG GGCATCTGTA 
CTTGGACAAA GAACTTCGGA ACTTCCATCA GCAATATCAT CTAGTAGCGG GCTGAAGGTT 
TCTGTGAAGG TTGTCTCTTT GAATGTTCAG GCCGGACTTG TCGAACCCAT GTACGGTACC 
TTGTGTCTCT ATCATCGAGA GAAGCGGGAG AAATTATCAG AAAATTTTCA CTTCCGATGT 
TTACCATCAG AGTTCCAAGA TTGTGTAACT TGGCAGGAGG GTGGTAATTG TCTGAGAAGA 
GCTATATTTT CACTGGAGGC AGCGTCGCCA GCAATATGTT TGTTGATCCA GCTTGAAAAG 
CATGTGACTG AGGAAGGAGG AGTAACGGCT CATGTTTATT CTCGAAAAGA ACCTGTACAC 
TTGACCGAGA GAGAAAAGCA GAAGTTACAG GTCTGGGCGC GCGTGATGCC ATTCAGGGAG 
CCTTTCGCGT GGGCAGTAGT TGCTCTGTTT GATGCAAATA TAACAGGAGG TGTAGGAGGA 
TTCTCATCTT CTCCAAGCAG CCCACTTCCA CCAGGACTTT TTGGACCATC TTTGATGGAA 
GCATCCTTGG ATTCTGATGG ACGACTTATA GCTGATGCTA AGACAGAATC ATCTAGTGTG 
CCAGTTTTGG TTGATGTTCA GAGCCTTCGC CGGGTCAAAG AAAATTACAC GGAGGAAATG 
CTTCAGGATC CCAAGTACAA AGTGCACAAA CCCGTGAAGG CCATCCTTCG TCTCGAGATA 
GAGAGGTTAT CTACTGAAGA TCATGACCAA GATTCCATAT TTGAGTGTGG TAGTTTTGGT 
CGTGGGTCCA CTGATGGTGA TTCCCGCTTG ACAGTAGGAC CCTCTTTCTC AGGGCGTTTC 
ATGCAGGGGC TGGGCATGAC GAAGGTTACG CATAATGGGC GGCAGAAATG GAGCCCTGGA 
AGAAAACATC GCTATCTGCA TTCCGGCAGT GTTGGCAGCA TTGAATTTGG AAAGTTTGAC 
TTCCGGGCTA TTGAGTTTCG ATCGGGTTCG AAAGTTGAGG TTTTACCTCA GCTATTGCAC 
TGCCTGTATG TATATCCTCA ATCAGTCAAT TTAAGTCGAA AACGCAATCT TTTTGTGCGA 
GTGGAGCTTC GAAAGGATGA TGTAGACATT CGCAAATCTC CTCTCGAGGC CCTTTATCCG 
AGGGATGCTG ACAGCTCTAT GCAGAAATAT GCACACTCTC AGATCGATGC AAACACCAAG 
ACACCTCACT ATCATGATGA GTTCAAAATT CAACTACCTG CCGTTCTCTC TCCTGAGCAT 
CACCTTCTGT TCACGTTTTT TCATGTGGAT CTACAGATGA AAATTGAGGC TCCAAAGCCG 
GTGGTAGTGG GATACTCTGT TCTTCCGCTC ATGTTTGTTT GTCAGGTTCA GCGGTTGGAC 
GGCAGCCTAC CTATCGCAAA GGAGCTGTAT TCCAATTATC TACAAGAAAA TGTGAAGGAA 
AGGTTGGAGT ACTTGGATGA TGGCAAAACT GTATTTAAAT TGAGGTCACG ATTATGCTCT 
TCTCTTTATC CGGTTAATGA GAGGATCCGA GATTTCTTTT CAGAATACGA TCGACATGTC 
TTGCGGACCA GTCCACCCTG GGGTACTGAG CTGATGGAGG CTATTAATGC TCTGAAGACG 
GTTGAGCCGT CAGCTATGCT CCAGTTTCTT CAACCTATTC TCAACATGCT GCTTCGTATG 
ATTCGCGATG GAGGAGAAAC GTTGCAGGTT GCTGCATTCC GTTCAATGGT TAACATCATT 
ACCAGGGTAC AAGCAGAGAC ATCTGATGGG GCTGAAAGAA ATCGTTACCT TGTGCATTAT 
GTGGATTATG CATTTAATGA TTTTGGTGGG CGTCATGAGC CTGTGTACCC AGGTCTGTGC 
AGTGTATGGC GCAGCCTTGC TCGAAGCAAG GCGAAAGGCT ACCGTGTCGG ACCTGTGTAT 
GATGATGTTC TTTCCATGGC TTGGGTGTTC CTGGAGCTAA TTGTCAAGTC CATGGCACTT 
GAACAATCCC GCATTTACTC TGAGTCCCTC CCACCTGGAG AGGAGTTACC ACCTTTGCAG 
CTCATTGATG AGGTCTTTAA AAGTATCGTA CAGCTCTATG ATTGTTTACT GACAGAGGTT 
CATGAGCGCT GCAAGAAGGG CATGCTTCTC GCAATGAAGC TGAATAGCAG TATTGCATAC 
TTTTGCTATG ACCTTCTCTC TGTAATTGAG CATCGACAAG TCTTTGAGCT GGTTGCGCTT 
TATTTCAACA AGTTTGCTGG GGTATGTCAA TCTCTTGTGC ACGAATGTAA GCTTAATTTT 
CTGCGCATCG TCTGTGACCA TGATCTCTTT GTTGAGATGC CCGGTCGAGA TCCTACAGAG 
AGGAATTATT TGGCATCACT GTTGATGCAA GAGCTCTTTG TTACTTGGGA TCATGAAGAT 
CTGACTTTAA GATCTAAGGC AGCTCGAATC CTTGTGCAAC TTATGTGCAA GCATGAGTAT 
GATGCACGGT ATCAAACATT GGAGGACAAA ATGTATATTG CCCAACGTTA CTTTCCACTA 
GTTGACCTGA TATTGGACGA AATGCCAGTG TTCTATGGCC TGAGCACTAC AGAGAAGCGT 
GAAGTGCTGG TGTGTGTGCT TCACATTCTG CGATATCTAG ACGATGCATC TCTTGTTAAA 
GCTTGGCAGC ACAACATGGA TAGAACAAAG CTTTTTTTCA AACTCCTGGA AGAATGTCAG 
GAATTGCTGG AGTATAAGAA GGCTGGAGCA GATGGCTTGA TGGGAGCTAT GCCAGCGAAT 
GATCAAGGAG AAGGGCCCCT TCGATACTCC GAGAAGCTCT CACCTGCAGT CAATCAGTTT 
TTGATTGAGG CATCTCGGCA GGATGTCAGA CTCACAAAGA TTTCTCCAAA TGCTCAGTGG 
ATACTTCCAC AAACGAGTCC AGAGATTGGT TCATTCTGGA AAAGAGCAAG CCCTGTAACA 
AATAGTTCAA CTAGCCCAGG TCAGCCGCAT TCGTTGCGAG AGGCACTAGC CCAAGCACAG 
TCTGCGGGAA GAAGTGGAGG CAGGTCTCTT CGTGAGACCT TGCATCCTAT GCTCCGCCAA 
AAGTTGGAGC TATGGGAGGA GAACCTTAGT GCCTCTGCTA TGCTCCAAAT TTTGGAGGTA 
CTTGAGAAGT TTATAAGTGC AGTATCAAGT CAAGCTGTTC TCACTGACTA CATGAGGCTT 
GATTGTATCA CAACTATTTT TATTGGTTTT CTGGGACGCA GCCAGCCGTT GCCATTTTGG 
AAGGCCTTTC TACCTTTCTT CAATAACGTG TTTCGTCGTC ATGGCGCTAC CTTGATGAGT 
AGGGAGAATG ATAGGTTCTT GAAGCAAGTA GCATTTCATC TACTACGGCT CGGAGTTTTC 
CGGAATGAGT CCATACGGAA ACGTTCTGTT GTTGGCCTTC AAATTCTTGT CCGAAATGCA 
TACCACTACT TTCAAGGCCT TTCAAGACTG CGGGTGATGC TTACAATCAC ACTTTCTGAG 
CTCATGTCAG ATGTTCAAGT AACACATCCT AAGATGGATG GATCCTTGGA AGAGAGTGGT 
GAATCTCAAC GTCTTCGTAG GTCCTTGCAA CAGATTGCAC AAGAAAGTAT TAGTGCGGAT 
CTTCTACGGG AATGTGGTCT TCCTGAAGAT TCTCTTACTG CTTTGCCAGA AGGTGTGAAT 
GGCCAACAAT GGAGTTGGGC TGGAGTCGCT GAGCTTTCCA CCTCTCTGCT GAAAGCTGTG 
GATGCTGCTG TAGGACATGC TCTTTTGGGT CCTGAGATTT TAATGAATGA CAAGTATGCA 
ACTGCAGAGG CCTACCATGG TTTGGCGACG GCATATACCA ACGTGCCCGA TCTGCATATA 
ATGTGGCTTC TGTATCTCTG CGAAGTTCAT CAAGGCAACC AGTCTTATGC AGAGGCTGCT 
CAGTGTGCTG TTGCGGTTGC AGGTGTCATC ATGCATGCTA TAGTAGGAAA AGGTGATCAT 
GTTTGGGGAA AAGAGCATGT TGAGGCACTG GGTAATATTT TCCCAATGCT CACTGGTTCC 
CGCTTTGGGG ATGCTGCAAC TGCCGAAATT GAAGGCTATG GGTCATCGAA ATTGACTGTC 
GAATCGGCAG TGAAGTACTT GCAGCTCGCC AATAAACTCT TTGTCCAGGC AGAGCTCTTC 
CATTTCTGTG CCGGCATATT GGAGCTCATA ATTCCCGTCC ATAAGTCAAG GCGTGCCTAT 
AGTCAGCTGT CTAAATGTCA TACTTCATTG ACCAGCATCT ACGAAGCTAT ACTAGAGCAA 
GAGTCGAGCC CTATCCCTTT CACGGATGCA ACATATTATC GTGTGGGATT TTATGGGAAA 
AGCTTTGGTA GTTTGCACTG CAAGGAGTAT GTGTACAGGG AACCACGGGA CGTGCGGTTA 
GGGGACATTA TGAAAAATTT AGGAAATATT TATGAGCCTA CAGTAATTGA AGGAAAGCAG 
AACTTGCATA TTATCCCCGA TTCTCGTCAA GTGAATCCAG AGGATCTTCA AGCTGGCATT 
TGTTACTTGC AAATTACATC TGTCGATCCT GTTACAGAGG ACGAGGATTT GGAAAGCAGG 
CGAGAAAGAC AATCCGGGAG GTCCTCGTCA ACATTCAGTG CACGAGTCTT CGATCGATTT 
TTATTTGACA CTCCTTTCAC AAAGAATGGA AGATCCCAAG GAGGTTTGGA AGATCAGTGG 
AAGAGGCGTA CGGTTTTGTG GACTGAAGGG CCTTTTCCAT CTCTTGTAAA TCGTTTAATG 
GTTGTGAAGT CTGAGTCGCG AGAATTTTCG CCAATCGAAA ACGCCATAGG AATGATTGAG 
ACTCGTACAG CAGCTTTAGC AGGAGAGCTC GATGATCAAC GTATAAATGA GGGAGATCCT 
CTGCCCCGTT TACAAGGCCT GCAAAGAATC TTGCAAGGAA GTGTTGCAGT CCAGGTAAAT 
AGTGGAGTGC TAGGTGTCTG CACAGCTTTC TTGTCTGGGG AACCTTCAAC ACGATTGCGC 
TCACAAGAAC TACAGCAGCT GATAGCAGCA CTCTTGGAGT TTATGGCTGT GTGCAAGAAA 
GCAATTCGAG TACATGCACG ACTGATTGGA GAGGAGGACC AAGACTTCCA CTCGCAGCTT 
GTGATTGGAT TCCAATCACT CACAGCAGAG CTATCCCACT ATATTCCCGC CATCTTGTCA 
GATGTAACCA TACTTGGGAA ACACAGCAAT GTGCTCAAGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC112279271 ( XM_024513569.1 ) cDNA ORF clone, -> XP_024369337.1 Physcomitrella patens guanine nucleotide exchange factor SPIKE 1-like (LOC112279271), transcript variant X5, mRNA.

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CloneID	OPn41280
Clone ID Related Accession (Same CDS sequence)	XM_024513560.1 , XM_024513543.1 , XM_024513534.1 , XM_024513569.1 , XM_024513576.1 , XM_024513552.1 , XM_024513585.1
Accession Version	XM_024513569.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Physcomitrella patens
Product	guanine nucleotide exchange factor SPIKE 1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037253.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physcomitrella patens Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATTTGG TCGCTGGAGC GCCTATATGG TCGGTGGAAA GCGTCAAGGA GGGAAAAGGA 
TCCAGGTTCG ACGTGTCGGA CTCAGTAGGA CAATGTAGGG TAGGTAGTCA TGATCGGAGG 
GACGAGGAGG AGACATGGGG ACTCAATGCA GAGACAGAAA GCTCTGCATT CTGGGAGCTG 
AGCTTCGAGA CTAGAGCTGT CGTCGACAGC GGATCAATTA AGAAGCAGGG GATGGATGAA 
ATTGCCTTGA GCGGGCAGCG GTTCAAACGT ATCCCACACC CGTACATCGG GCAGTCAACA 
GATCTTGAGT CCTTGCTGAA TGGGAATGGA GACCCTTTCC CACATTTCAA AGAGCTTCTT 
CAAAGCTACA GGGCTGAATG GGTGAAGGAT GAGTTCAAAT ACGGTCATTA TGAAGCTGTG 
CCACCGCCAG CCTTAGAACA TCAGATTTTT GAAGGACCCG ACACTGACAT ACAGACAGAG 
TTTCGGCTTT CTAACGTCAG ACTCGGCAGA AATGGTGATG TTACTGATGA TGAAGATCCT 
CAAACTCCAC GACGGCTTTC AAGACGTTTT TCTGTACGGT CATCTGGTCG TTTCTCTATG 
AGATCTACGA CTAGTGATGC ACCTGAAGCA TTGCACACAA ATATCTCAAA GCATTATGGG 
GTTGCTCCGT TACCTCCTTA TGAGCCCGTT TATAATTGGC GAGCAACAAG GGCATCTGTA 
CTTGGACAAA GAACTTCGGA ACTTCCATCA GCAATATCAT CTAGTAGCGG GCTGAAGGTT 
TCTGTGAAGG TTGTCTCTTT GAATGTTCAG GCCGGACTTG TCGAACCCAT GTACGGTACC 
TTGTGTCTCT ATCATCGAGA GAAGCGGGAG AAATTATCAG AAAATTTTCA CTTCCGATGT 
TTACCATCAG AGTTCCAAGA TTGTGTAACT TGGCAGGAGG GTGGTAATTG TCTGAGAAGA 
GCTATATTTT CACTGGAGGC AGCGTCGCCA GCAATATGTT TGTTGATCCA GCTTGAAAAG 
CATGTGACTG AGGAAGGAGG AGTAACGGCT CATGTTTATT CTCGAAAAGA ACCTGTACAC 
TTGACCGAGA GAGAAAAGCA GAAGTTACAG GTCTGGGCGC GCGTGATGCC ATTCAGGGAG 
CCTTTCGCGT GGGCAGTAGT TGCTCTGTTT GATGCAAATA TAACAGGAGG TGTAGGAGGA 
TTCTCATCTT CTCCAAGCAG CCCACTTCCA CCAGGACTTT TTGGACCATC TTTGATGGAA 
GCATCCTTGG ATTCTGATGG ACGACTTATA GCTGATGCTA AGACAGAATC ATCTAGTGTG 
CCAGTTTTGG TTGATGTTCA GAGCCTTCGC CGGGTCAAAG AAAATTACAC GGAGGAAATG 
CTTCAGGATC CCAAGTACAA AGTGCACAAA CCCGTGAAGG CCATCCTTCG TCTCGAGATA 
GAGAGGTTAT CTACTGAAGA TCATGACCAA GATTCCATAT TTGAGTGTGG TAGTTTTGGT 
CGTGGGTCCA CTGATGGTGA TTCCCGCTTG ACAGTAGGAC CCTCTTTCTC AGGGCGTTTC 
ATGCAGGGGC TGGGCATGAC GAAGGTTACG CATAATGGGC GGCAGAAATG GAGCCCTGGA 
AGAAAACATC GCTATCTGCA TTCCGGCAGT GTTGGCAGCA TTGAATTTGG AAAGTTTGAC 
TTCCGGGCTA TTGAGTTTCG ATCGGGTTCG AAAGTTGAGG TTTTACCTCA GCTATTGCAC 
TGCCTGTATG TATATCCTCA ATCAGTCAAT TTAAGTCGAA AACGCAATCT TTTTGTGCGA 
GTGGAGCTTC GAAAGGATGA TGTAGACATT CGCAAATCTC CTCTCGAGGC CCTTTATCCG 
AGGGATGCTG ACAGCTCTAT GCAGAAATAT GCACACTCTC AGATCGATGC AAACACCAAG 
ACACCTCACT ATCATGATGA GTTCAAAATT CAACTACCTG CCGTTCTCTC TCCTGAGCAT 
CACCTTCTGT TCACGTTTTT TCATGTGGAT CTACAGATGA AAATTGAGGC TCCAAAGCCG 
GTGGTAGTGG GATACTCTGT TCTTCCGCTC ATGTTTGTTT GTCAGGTTCA GCGGTTGGAC 
GGCAGCCTAC CTATCGCAAA GGAGCTGTAT TCCAATTATC TACAAGAAAA TGTGAAGGAA 
AGGTTGGAGT ACTTGGATGA TGGCAAAACT GTATTTAAAT TGAGGTCACG ATTATGCTCT 
TCTCTTTATC CGGTTAATGA GAGGATCCGA GATTTCTTTT CAGAATACGA TCGACATGTC 
TTGCGGACCA GTCCACCCTG GGGTACTGAG CTGATGGAGG CTATTAATGC TCTGAAGACG 
GTTGAGCCGT CAGCTATGCT CCAGTTTCTT CAACCTATTC TCAACATGCT GCTTCGTATG 
ATTCGCGATG GAGGAGAAAC GTTGCAGGTT GCTGCATTCC GTTCAATGGT TAACATCATT 
ACCAGGGTAC AAGCAGAGAC ATCTGATGGG GCTGAAAGAA ATCGTTACCT TGTGCATTAT 
GTGGATTATG CATTTAATGA TTTTGGTGGG CGTCATGAGC CTGTGTACCC AGGTCTGTGC 
AGTGTATGGC GCAGCCTTGC TCGAAGCAAG GCGAAAGGCT ACCGTGTCGG ACCTGTGTAT 
GATGATGTTC TTTCCATGGC TTGGGTGTTC CTGGAGCTAA TTGTCAAGTC CATGGCACTT 
GAACAATCCC GCATTTACTC TGAGTCCCTC CCACCTGGAG AGGAGTTACC ACCTTTGCAG 
CTCATTGATG AGGTCTTTAA AAGTATCGTA CAGCTCTATG ATTGTTTACT GACAGAGGTT 
CATGAGCGCT GCAAGAAGGG CATGCTTCTC GCAATGAAGC TGAATAGCAG TATTGCATAC 
TTTTGCTATG ACCTTCTCTC TGTAATTGAG CATCGACAAG TCTTTGAGCT GGTTGCGCTT 
TATTTCAACA AGTTTGCTGG GGTATGTCAA TCTCTTGTGC ACGAATGTAA GCTTAATTTT 
CTGCGCATCG TCTGTGACCA TGATCTCTTT GTTGAGATGC CCGGTCGAGA TCCTACAGAG 
AGGAATTATT TGGCATCACT GTTGATGCAA GAGCTCTTTG TTACTTGGGA TCATGAAGAT 
CTGACTTTAA GATCTAAGGC AGCTCGAATC CTTGTGCAAC TTATGTGCAA GCATGAGTAT 
GATGCACGGT ATCAAACATT GGAGGACAAA ATGTATATTG CCCAACGTTA CTTTCCACTA 
GTTGACCTGA TATTGGACGA AATGCCAGTG TTCTATGGCC TGAGCACTAC AGAGAAGCGT 
GAAGTGCTGG TGTGTGTGCT TCACATTCTG CGATATCTAG ACGATGCATC TCTTGTTAAA 
GCTTGGCAGC ACAACATGGA TAGAACAAAG CTTTTTTTCA AACTCCTGGA AGAATGTCAG 
GAATTGCTGG AGTATAAGAA GGCTGGAGCA GATGGCTTGA TGGGAGCTAT GCCAGCGAAT 
GATCAAGGAG AAGGGCCCCT TCGATACTCC GAGAAGCTCT CACCTGCAGT CAATCAGTTT 
TTGATTGAGG CATCTCGGCA GGATGTCAGA CTCACAAAGA TTTCTCCAAA TGCTCAGTGG 
ATACTTCCAC AAACGAGTCC AGAGATTGGT TCATTCTGGA AAAGAGCAAG CCCTGTAACA 
AATAGTTCAA CTAGCCCAGG TCAGCCGCAT TCGTTGCGAG AGGCACTAGC CCAAGCACAG 
TCTGCGGGAA GAAGTGGAGG CAGGTCTCTT CGTGAGACCT TGCATCCTAT GCTCCGCCAA 
AAGTTGGAGC TATGGGAGGA GAACCTTAGT GCCTCTGCTA TGCTCCAAAT TTTGGAGGTA 
CTTGAGAAGT TTATAAGTGC AGTATCAAGT CAAGCTGTTC TCACTGACTA CATGAGGCTT 
GATTGTATCA CAACTATTTT TATTGGTTTT CTGGGACGCA GCCAGCCGTT GCCATTTTGG 
AAGGCCTTTC TACCTTTCTT CAATAACGTG TTTCGTCGTC ATGGCGCTAC CTTGATGAGT 
AGGGAGAATG ATAGGTTCTT GAAGCAAGTA GCATTTCATC TACTACGGCT CGGAGTTTTC 
CGGAATGAGT CCATACGGAA ACGTTCTGTT GTTGGCCTTC AAATTCTTGT CCGAAATGCA 
TACCACTACT TTCAAGGCCT TTCAAGACTG CGGGTGATGC TTACAATCAC ACTTTCTGAG 
CTCATGTCAG ATGTTCAAGT AACACATCCT AAGATGGATG GATCCTTGGA AGAGAGTGGT 
GAATCTCAAC GTCTTCGTAG GTCCTTGCAA CAGATTGCAC AAGAAAGTAT TAGTGCGGAT 
CTTCTACGGG AATGTGGTCT TCCTGAAGAT TCTCTTACTG CTTTGCCAGA AGGTGTGAAT 
GGCCAACAAT GGAGTTGGGC TGGAGTCGCT GAGCTTTCCA CCTCTCTGCT GAAAGCTGTG 
GATGCTGCTG TAGGACATGC TCTTTTGGGT CCTGAGATTT TAATGAATGA CAAGTATGCA 
ACTGCAGAGG CCTACCATGG TTTGGCGACG GCATATACCA ACGTGCCCGA TCTGCATATA 
ATGTGGCTTC TGTATCTCTG CGAAGTTCAT CAAGGCAACC AGTCTTATGC AGAGGCTGCT 
CAGTGTGCTG TTGCGGTTGC AGGTGTCATC ATGCATGCTA TAGTAGGAAA AGGTGATCAT 
GTTTGGGGAA AAGAGCATGT TGAGGCACTG GGTAATATTT TCCCAATGCT CACTGGTTCC 
CGCTTTGGGG ATGCTGCAAC TGCCGAAATT GAAGGCTATG GGTCATCGAA ATTGACTGTC 
GAATCGGCAG TGAAGTACTT GCAGCTCGCC AATAAACTCT TTGTCCAGGC AGAGCTCTTC 
CATTTCTGTG CCGGCATATT GGAGCTCATA ATTCCCGTCC ATAAGTCAAG GCGTGCCTAT 
AGTCAGCTGT CTAAATGTCA TACTTCATTG ACCAGCATCT ACGAAGCTAT ACTAGAGCAA 
GAGTCGAGCC CTATCCCTTT CACGGATGCA ACATATTATC GTGTGGGATT TTATGGGAAA 
AGCTTTGGTA GTTTGCACTG CAAGGAGTAT GTGTACAGGG AACCACGGGA CGTGCGGTTA 
GGGGACATTA TGAAAAATTT AGGAAATATT TATGAGCCTA CAGTAATTGA AGGAAAGCAG 
AACTTGCATA TTATCCCCGA TTCTCGTCAA GTGAATCCAG AGGATCTTCA AGCTGGCATT 
TGTTACTTGC AAATTACATC TGTCGATCCT GTTACAGAGG ACGAGGATTT GGAAAGCAGG 
CGAGAAAGAC AATCCGGGAG GTCCTCGTCA ACATTCAGTG CACGAGTCTT CGATCGATTT 
TTATTTGACA CTCCTTTCAC AAAGAATGGA AGATCCCAAG GAGGTTTGGA AGATCAGTGG 
AAGAGGCGTA CGGTTTTGTG GACTGAAGGG CCTTTTCCAT CTCTTGTAAA TCGTTTAATG 
GTTGTGAAGT CTGAGTCGCG AGAATTTTCG CCAATCGAAA ACGCCATAGG AATGATTGAG 
ACTCGTACAG CAGCTTTAGC AGGAGAGCTC GATGATCAAC GTATAAATGA GGGAGATCCT 
CTGCCCCGTT TACAAGGCCT GCAAAGAATC TTGCAAGGAA GTGTTGCAGT CCAGGTAAAT 
AGTGGAGTGC TAGGTGTCTG CACAGCTTTC TTGTCTGGGG AACCTTCAAC ACGATTGCGC 
TCACAAGAAC TACAGCAGCT GATAGCAGCA CTCTTGGAGT TTATGGCTGT GTGCAAGAAA 
GCAATTCGAG TACATGCACG ACTGATTGGA GAGGAGGACC AAGACTTCCA CTCGCAGCTT 
GTGATTGGAT TCCAATCACT CACAGCAGAG CTATCCCACT ATATTCCCGC CATCTTGTCA 
GATGTAACCA TACTTGGGAA ACACAGCAAT GTGCTCAAGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024369337.1
CDS	411..6335
Translation	MHLVAGAPIWSVESVKEGKGSRFDVSDSVGQCRVGSHDRRDEEETWGLNAETESSAFWELSFETRAVVDSGSIKKQGMDEIALSGQRFKRIPHPYIGQSTDLESLLNGNGDPFPHFKELLQSYRAEWVKDEFKYGHYEAVPPPALEHQIFEGPDTDIQTEFRLSNVRLGRNGDVTDDEDPQTPRRLSRRFSVRSSGRFSMRSTTSDAPEALHTNISKHYGVAPLPPYEPVYNWRATRASVLGQRTSELPSAISSSSGLKVSVKVVSLNVQAGLVEPMYGTLCLYHREKREKLSENFHFRCLPSEFQDCVTWQEGGNCLRRAIFSLEAASPAICLLIQLEKHVTEEGGVTAHVYSRKEPVHLTEREKQKLQVWARVMPFREPFAWAVVALFDANITGGVGGFSSSPSSPLPPGLFGPSLMEASLDSDGRLIADAKTESSSVPVLVDVQSLRRVKENYTEEMLQDPKYKVHKPVKAILRLEIERLSTEDHDQDSIFECGSFGRGSTDGDSRLTVGPSFSGRFMQGLGMTKVTHNGRQKWSPGRKHRYLHSGSVGSIEFGKFDFRAIEFRSGSKVEVLPQLLHCLYVYPQSVNLSRKRNLFVRVELRKDDVDIRKSPLEALYPRDADSSMQKYAHSQIDANTKTPHYHDEFKIQLPAVLSPEHHLLFTFFHVDLQMKIEAPKPVVVGYSVLPLMFVCQVQRLDGSLPIAKELYSNYLQENVKERLEYLDDGKTVFKLRSRLCSSLYPVNERIRDFFSEYDRHVLRTSPPWGTELMEAINALKTVEPSAMLQFLQPILNMLLRMIRDGGETLQVAAFRSMVNIITRVQAETSDGAERNRYLVHYVDYAFNDFGGRHEPVYPGLCSVWRSLARSKAKGYRVGPVYDDVLSMAWVFLELIVKSMALEQSRIYSESLPPGEELPPLQLIDEVFKSIVQLYDCLLTEVHERCKKGMLLAMKLNSSIAYFCYDLLSVIEHRQVFELVALYFNKFAGVCQSLVHECKLNFLRIVCDHDLFVEMPGRDPTERNYLASLLMQELFVTWDHEDLTLRSKAARILVQLMCKHEYDARYQTLEDKMYIAQRYFPLVDLILDEMPVFYGLSTTEKREVLVCVLHILRYLDDASLVKAWQHNMDRTKLFFKLLEECQELLEYKKAGADGLMGAMPANDQGEGPLRYSEKLSPAVNQFLIEASRQDVRLTKISPNAQWILPQTSPEIGSFWKRASPVTNSSTSPGQPHSLREALAQAQSAGRSGGRSLRETLHPMLRQKLELWEENLSASAMLQILEVLEKFISAVSSQAVLTDYMRLDCITTIFIGFLGRSQPLPFWKAFLPFFNNVFRRHGATLMSRENDRFLKQVAFHLLRLGVFRNESIRKRSVVGLQILVRNAYHYFQGLSRLRVMLTITLSELMSDVQVTHPKMDGSLEESGESQRLRRSLQQIAQESISADLLRECGLPEDSLTALPEGVNGQQWSWAGVAELSTSLLKAVDAAVGHALLGPEILMNDKYATAEAYHGLATAYTNVPDLHIMWLLYLCEVHQGNQSYAEAAQCAVAVAGVIMHAIVGKGDHVWGKEHVEALGNIFPMLTGSRFGDAATAEIEGYGSSKLTVESAVKYLQLANKLFVQAELFHFCAGILELIIPVHKSRRAYSQLSKCHTSLTSIYEAILEQESSPIPFTDATYYRVGFYGKSFGSLHCKEYVYREPRDVRLGDIMKNLGNIYEPTVIEGKQNLHIIPDSRQVNPEDLQAGICYLQITSVDPVTEDEDLESRRERQSGRSSSTFSARVFDRFLFDTPFTKNGRSQGGLEDQWKRRTVLWTEGPFPSLVNRLMVVKSESREFSPIENAIGMIETRTAALAGELDDQRINEGDPLPRLQGLQRILQGSVAVQVNSGVLGVCTAFLSGEPSTRLRSQELQQLIAALLEFMAVCKKAIRVHARLIGEEDQDFHSQLVIGFQSLTAELSHYIPAILSDVTILGKHSNVLKL

Target ORF information:

RefSeq Version	XM_024513569.1
Organism	Physcomitrella patens
Definition	Physcomitrella patens guanine nucleotide exchange factor SPIKE 1-like (LOC112279271), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024513569.1

ORF Insert Sequence:

ATGCATTTGG TCGCTGGAGC GCCTATATGG TCGGTGGAAA GCGTCAAGGA GGGAAAAGGA 
TCCAGGTTCG ACGTGTCGGA CTCAGTAGGA CAATGTAGGG TAGGTAGTCA TGATCGGAGG 
GACGAGGAGG AGACATGGGG ACTCAATGCA GAGACAGAAA GCTCTGCATT CTGGGAGCTG 
AGCTTCGAGA CTAGAGCTGT CGTCGACAGC GGATCAATTA AGAAGCAGGG GATGGATGAA 
ATTGCCTTGA GCGGGCAGCG GTTCAAACGT ATCCCACACC CGTACATCGG GCAGTCAACA 
GATCTTGAGT CCTTGCTGAA TGGGAATGGA GACCCTTTCC CACATTTCAA AGAGCTTCTT 
CAAAGCTACA GGGCTGAATG GGTGAAGGAT GAGTTCAAAT ACGGTCATTA TGAAGCTGTG 
CCACCGCCAG CCTTAGAACA TCAGATTTTT GAAGGACCCG ACACTGACAT ACAGACAGAG 
TTTCGGCTTT CTAACGTCAG ACTCGGCAGA AATGGTGATG TTACTGATGA TGAAGATCCT 
CAAACTCCAC GACGGCTTTC AAGACGTTTT TCTGTACGGT CATCTGGTCG TTTCTCTATG 
AGATCTACGA CTAGTGATGC ACCTGAAGCA TTGCACACAA ATATCTCAAA GCATTATGGG 
GTTGCTCCGT TACCTCCTTA TGAGCCCGTT TATAATTGGC GAGCAACAAG GGCATCTGTA 
CTTGGACAAA GAACTTCGGA ACTTCCATCA GCAATATCAT CTAGTAGCGG GCTGAAGGTT 
TCTGTGAAGG TTGTCTCTTT GAATGTTCAG GCCGGACTTG TCGAACCCAT GTACGGTACC 
TTGTGTCTCT ATCATCGAGA GAAGCGGGAG AAATTATCAG AAAATTTTCA CTTCCGATGT 
TTACCATCAG AGTTCCAAGA TTGTGTAACT TGGCAGGAGG GTGGTAATTG TCTGAGAAGA 
GCTATATTTT CACTGGAGGC AGCGTCGCCA GCAATATGTT TGTTGATCCA GCTTGAAAAG 
CATGTGACTG AGGAAGGAGG AGTAACGGCT CATGTTTATT CTCGAAAAGA ACCTGTACAC 
TTGACCGAGA GAGAAAAGCA GAAGTTACAG GTCTGGGCGC GCGTGATGCC ATTCAGGGAG 
CCTTTCGCGT GGGCAGTAGT TGCTCTGTTT GATGCAAATA TAACAGGAGG TGTAGGAGGA 
TTCTCATCTT CTCCAAGCAG CCCACTTCCA CCAGGACTTT TTGGACCATC TTTGATGGAA 
GCATCCTTGG ATTCTGATGG ACGACTTATA GCTGATGCTA AGACAGAATC ATCTAGTGTG 
CCAGTTTTGG TTGATGTTCA GAGCCTTCGC CGGGTCAAAG AAAATTACAC GGAGGAAATG 
CTTCAGGATC CCAAGTACAA AGTGCACAAA CCCGTGAAGG CCATCCTTCG TCTCGAGATA 
GAGAGGTTAT CTACTGAAGA TCATGACCAA GATTCCATAT TTGAGTGTGG TAGTTTTGGT 
CGTGGGTCCA CTGATGGTGA TTCCCGCTTG ACAGTAGGAC CCTCTTTCTC AGGGCGTTTC 
ATGCAGGGGC TGGGCATGAC GAAGGTTACG CATAATGGGC GGCAGAAATG GAGCCCTGGA 
AGAAAACATC GCTATCTGCA TTCCGGCAGT GTTGGCAGCA TTGAATTTGG AAAGTTTGAC 
TTCCGGGCTA TTGAGTTTCG ATCGGGTTCG AAAGTTGAGG TTTTACCTCA GCTATTGCAC 
TGCCTGTATG TATATCCTCA ATCAGTCAAT TTAAGTCGAA AACGCAATCT TTTTGTGCGA 
GTGGAGCTTC GAAAGGATGA TGTAGACATT CGCAAATCTC CTCTCGAGGC CCTTTATCCG 
AGGGATGCTG ACAGCTCTAT GCAGAAATAT GCACACTCTC AGATCGATGC AAACACCAAG 
ACACCTCACT ATCATGATGA GTTCAAAATT CAACTACCTG CCGTTCTCTC TCCTGAGCAT 
CACCTTCTGT TCACGTTTTT TCATGTGGAT CTACAGATGA AAATTGAGGC TCCAAAGCCG 
GTGGTAGTGG GATACTCTGT TCTTCCGCTC ATGTTTGTTT GTCAGGTTCA GCGGTTGGAC 
GGCAGCCTAC CTATCGCAAA GGAGCTGTAT TCCAATTATC TACAAGAAAA TGTGAAGGAA 
AGGTTGGAGT ACTTGGATGA TGGCAAAACT GTATTTAAAT TGAGGTCACG ATTATGCTCT 
TCTCTTTATC CGGTTAATGA GAGGATCCGA GATTTCTTTT CAGAATACGA TCGACATGTC 
TTGCGGACCA GTCCACCCTG GGGTACTGAG CTGATGGAGG CTATTAATGC TCTGAAGACG 
GTTGAGCCGT CAGCTATGCT CCAGTTTCTT CAACCTATTC TCAACATGCT GCTTCGTATG 
ATTCGCGATG GAGGAGAAAC GTTGCAGGTT GCTGCATTCC GTTCAATGGT TAACATCATT 
ACCAGGGTAC AAGCAGAGAC ATCTGATGGG GCTGAAAGAA ATCGTTACCT TGTGCATTAT 
GTGGATTATG CATTTAATGA TTTTGGTGGG CGTCATGAGC CTGTGTACCC AGGTCTGTGC 
AGTGTATGGC GCAGCCTTGC TCGAAGCAAG GCGAAAGGCT ACCGTGTCGG ACCTGTGTAT 
GATGATGTTC TTTCCATGGC TTGGGTGTTC CTGGAGCTAA TTGTCAAGTC CATGGCACTT 
GAACAATCCC GCATTTACTC TGAGTCCCTC CCACCTGGAG AGGAGTTACC ACCTTTGCAG 
CTCATTGATG AGGTCTTTAA AAGTATCGTA CAGCTCTATG ATTGTTTACT GACAGAGGTT 
CATGAGCGCT GCAAGAAGGG CATGCTTCTC GCAATGAAGC TGAATAGCAG TATTGCATAC 
TTTTGCTATG ACCTTCTCTC TGTAATTGAG CATCGACAAG TCTTTGAGCT GGTTGCGCTT 
TATTTCAACA AGTTTGCTGG GGTATGTCAA TCTCTTGTGC ACGAATGTAA GCTTAATTTT 
CTGCGCATCG TCTGTGACCA TGATCTCTTT GTTGAGATGC CCGGTCGAGA TCCTACAGAG 
AGGAATTATT TGGCATCACT GTTGATGCAA GAGCTCTTTG TTACTTGGGA TCATGAAGAT 
CTGACTTTAA GATCTAAGGC AGCTCGAATC CTTGTGCAAC TTATGTGCAA GCATGAGTAT 
GATGCACGGT ATCAAACATT GGAGGACAAA ATGTATATTG CCCAACGTTA CTTTCCACTA 
GTTGACCTGA TATTGGACGA AATGCCAGTG TTCTATGGCC TGAGCACTAC AGAGAAGCGT 
GAAGTGCTGG TGTGTGTGCT TCACATTCTG CGATATCTAG ACGATGCATC TCTTGTTAAA 
GCTTGGCAGC ACAACATGGA TAGAACAAAG CTTTTTTTCA AACTCCTGGA AGAATGTCAG 
GAATTGCTGG AGTATAAGAA GGCTGGAGCA GATGGCTTGA TGGGAGCTAT GCCAGCGAAT 
GATCAAGGAG AAGGGCCCCT TCGATACTCC GAGAAGCTCT CACCTGCAGT CAATCAGTTT 
TTGATTGAGG CATCTCGGCA GGATGTCAGA CTCACAAAGA TTTCTCCAAA TGCTCAGTGG 
ATACTTCCAC AAACGAGTCC AGAGATTGGT TCATTCTGGA AAAGAGCAAG CCCTGTAACA 
AATAGTTCAA CTAGCCCAGG TCAGCCGCAT TCGTTGCGAG AGGCACTAGC CCAAGCACAG 
TCTGCGGGAA GAAGTGGAGG CAGGTCTCTT CGTGAGACCT TGCATCCTAT GCTCCGCCAA 
AAGTTGGAGC TATGGGAGGA GAACCTTAGT GCCTCTGCTA TGCTCCAAAT TTTGGAGGTA 
CTTGAGAAGT TTATAAGTGC AGTATCAAGT CAAGCTGTTC TCACTGACTA CATGAGGCTT 
GATTGTATCA CAACTATTTT TATTGGTTTT CTGGGACGCA GCCAGCCGTT GCCATTTTGG 
AAGGCCTTTC TACCTTTCTT CAATAACGTG TTTCGTCGTC ATGGCGCTAC CTTGATGAGT 
AGGGAGAATG ATAGGTTCTT GAAGCAAGTA GCATTTCATC TACTACGGCT CGGAGTTTTC 
CGGAATGAGT CCATACGGAA ACGTTCTGTT GTTGGCCTTC AAATTCTTGT CCGAAATGCA 
TACCACTACT TTCAAGGCCT TTCAAGACTG CGGGTGATGC TTACAATCAC ACTTTCTGAG 
CTCATGTCAG ATGTTCAAGT AACACATCCT AAGATGGATG GATCCTTGGA AGAGAGTGGT 
GAATCTCAAC GTCTTCGTAG GTCCTTGCAA CAGATTGCAC AAGAAAGTAT TAGTGCGGAT 
CTTCTACGGG AATGTGGTCT TCCTGAAGAT TCTCTTACTG CTTTGCCAGA AGGTGTGAAT 
GGCCAACAAT GGAGTTGGGC TGGAGTCGCT GAGCTTTCCA CCTCTCTGCT GAAAGCTGTG 
GATGCTGCTG TAGGACATGC TCTTTTGGGT CCTGAGATTT TAATGAATGA CAAGTATGCA 
ACTGCAGAGG CCTACCATGG TTTGGCGACG GCATATACCA ACGTGCCCGA TCTGCATATA 
ATGTGGCTTC TGTATCTCTG CGAAGTTCAT CAAGGCAACC AGTCTTATGC AGAGGCTGCT 
CAGTGTGCTG TTGCGGTTGC AGGTGTCATC ATGCATGCTA TAGTAGGAAA AGGTGATCAT 
GTTTGGGGAA AAGAGCATGT TGAGGCACTG GGTAATATTT TCCCAATGCT CACTGGTTCC 
CGCTTTGGGG ATGCTGCAAC TGCCGAAATT GAAGGCTATG GGTCATCGAA ATTGACTGTC 
GAATCGGCAG TGAAGTACTT GCAGCTCGCC AATAAACTCT TTGTCCAGGC AGAGCTCTTC 
CATTTCTGTG CCGGCATATT GGAGCTCATA ATTCCCGTCC ATAAGTCAAG GCGTGCCTAT 
AGTCAGCTGT CTAAATGTCA TACTTCATTG ACCAGCATCT ACGAAGCTAT ACTAGAGCAA 
GAGTCGAGCC CTATCCCTTT CACGGATGCA ACATATTATC GTGTGGGATT TTATGGGAAA 
AGCTTTGGTA GTTTGCACTG CAAGGAGTAT GTGTACAGGG AACCACGGGA CGTGCGGTTA 
GGGGACATTA TGAAAAATTT AGGAAATATT TATGAGCCTA CAGTAATTGA AGGAAAGCAG 
AACTTGCATA TTATCCCCGA TTCTCGTCAA GTGAATCCAG AGGATCTTCA AGCTGGCATT 
TGTTACTTGC AAATTACATC TGTCGATCCT GTTACAGAGG ACGAGGATTT GGAAAGCAGG 
CGAGAAAGAC AATCCGGGAG GTCCTCGTCA ACATTCAGTG CACGAGTCTT CGATCGATTT 
TTATTTGACA CTCCTTTCAC AAAGAATGGA AGATCCCAAG GAGGTTTGGA AGATCAGTGG 
AAGAGGCGTA CGGTTTTGTG GACTGAAGGG CCTTTTCCAT CTCTTGTAAA TCGTTTAATG 
GTTGTGAAGT CTGAGTCGCG AGAATTTTCG CCAATCGAAA ACGCCATAGG AATGATTGAG 
ACTCGTACAG CAGCTTTAGC AGGAGAGCTC GATGATCAAC GTATAAATGA GGGAGATCCT 
CTGCCCCGTT TACAAGGCCT GCAAAGAATC TTGCAAGGAA GTGTTGCAGT CCAGGTAAAT 
AGTGGAGTGC TAGGTGTCTG CACAGCTTTC TTGTCTGGGG AACCTTCAAC ACGATTGCGC 
TCACAAGAAC TACAGCAGCT GATAGCAGCA CTCTTGGAGT TTATGGCTGT GTGCAAGAAA 
GCAATTCGAG TACATGCACG ACTGATTGGA GAGGAGGACC AAGACTTCCA CTCGCAGCTT 
GTGATTGGAT TCCAATCACT CACAGCAGAG CTATCCCACT ATATTCCCGC CATCTTGTCA 
GATGTAACCA TACTTGGGAA ACACAGCAAT GTGCTCAAGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC112279271 ( XM_024513576.1 ) cDNA ORF clone, -> XP_024369344.1 Physcomitrella patens guanine nucleotide exchange factor SPIKE 1-like (LOC112279271), transcript variant X6, mRNA.

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CloneID	OPn41280
Clone ID Related Accession (Same CDS sequence)	XM_024513560.1 , XM_024513543.1 , XM_024513534.1 , XM_024513569.1 , XM_024513576.1 , XM_024513552.1 , XM_024513585.1
Accession Version	XM_024513576.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Physcomitrella patens
Product	guanine nucleotide exchange factor SPIKE 1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037253.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physcomitrella patens Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATTTGG TCGCTGGAGC GCCTATATGG TCGGTGGAAA GCGTCAAGGA GGGAAAAGGA 
TCCAGGTTCG ACGTGTCGGA CTCAGTAGGA CAATGTAGGG TAGGTAGTCA TGATCGGAGG 
GACGAGGAGG AGACATGGGG ACTCAATGCA GAGACAGAAA GCTCTGCATT CTGGGAGCTG 
AGCTTCGAGA CTAGAGCTGT CGTCGACAGC GGATCAATTA AGAAGCAGGG GATGGATGAA 
ATTGCCTTGA GCGGGCAGCG GTTCAAACGT ATCCCACACC CGTACATCGG GCAGTCAACA 
GATCTTGAGT CCTTGCTGAA TGGGAATGGA GACCCTTTCC CACATTTCAA AGAGCTTCTT 
CAAAGCTACA GGGCTGAATG GGTGAAGGAT GAGTTCAAAT ACGGTCATTA TGAAGCTGTG 
CCACCGCCAG CCTTAGAACA TCAGATTTTT GAAGGACCCG ACACTGACAT ACAGACAGAG 
TTTCGGCTTT CTAACGTCAG ACTCGGCAGA AATGGTGATG TTACTGATGA TGAAGATCCT 
CAAACTCCAC GACGGCTTTC AAGACGTTTT TCTGTACGGT CATCTGGTCG TTTCTCTATG 
AGATCTACGA CTAGTGATGC ACCTGAAGCA TTGCACACAA ATATCTCAAA GCATTATGGG 
GTTGCTCCGT TACCTCCTTA TGAGCCCGTT TATAATTGGC GAGCAACAAG GGCATCTGTA 
CTTGGACAAA GAACTTCGGA ACTTCCATCA GCAATATCAT CTAGTAGCGG GCTGAAGGTT 
TCTGTGAAGG TTGTCTCTTT GAATGTTCAG GCCGGACTTG TCGAACCCAT GTACGGTACC 
TTGTGTCTCT ATCATCGAGA GAAGCGGGAG AAATTATCAG AAAATTTTCA CTTCCGATGT 
TTACCATCAG AGTTCCAAGA TTGTGTAACT TGGCAGGAGG GTGGTAATTG TCTGAGAAGA 
GCTATATTTT CACTGGAGGC AGCGTCGCCA GCAATATGTT TGTTGATCCA GCTTGAAAAG 
CATGTGACTG AGGAAGGAGG AGTAACGGCT CATGTTTATT CTCGAAAAGA ACCTGTACAC 
TTGACCGAGA GAGAAAAGCA GAAGTTACAG GTCTGGGCGC GCGTGATGCC ATTCAGGGAG 
CCTTTCGCGT GGGCAGTAGT TGCTCTGTTT GATGCAAATA TAACAGGAGG TGTAGGAGGA 
TTCTCATCTT CTCCAAGCAG CCCACTTCCA CCAGGACTTT TTGGACCATC TTTGATGGAA 
GCATCCTTGG ATTCTGATGG ACGACTTATA GCTGATGCTA AGACAGAATC ATCTAGTGTG 
CCAGTTTTGG TTGATGTTCA GAGCCTTCGC CGGGTCAAAG AAAATTACAC GGAGGAAATG 
CTTCAGGATC CCAAGTACAA AGTGCACAAA CCCGTGAAGG CCATCCTTCG TCTCGAGATA 
GAGAGGTTAT CTACTGAAGA TCATGACCAA GATTCCATAT TTGAGTGTGG TAGTTTTGGT 
CGTGGGTCCA CTGATGGTGA TTCCCGCTTG ACAGTAGGAC CCTCTTTCTC AGGGCGTTTC 
ATGCAGGGGC TGGGCATGAC GAAGGTTACG CATAATGGGC GGCAGAAATG GAGCCCTGGA 
AGAAAACATC GCTATCTGCA TTCCGGCAGT GTTGGCAGCA TTGAATTTGG AAAGTTTGAC 
TTCCGGGCTA TTGAGTTTCG ATCGGGTTCG AAAGTTGAGG TTTTACCTCA GCTATTGCAC 
TGCCTGTATG TATATCCTCA ATCAGTCAAT TTAAGTCGAA AACGCAATCT TTTTGTGCGA 
GTGGAGCTTC GAAAGGATGA TGTAGACATT CGCAAATCTC CTCTCGAGGC CCTTTATCCG 
AGGGATGCTG ACAGCTCTAT GCAGAAATAT GCACACTCTC AGATCGATGC AAACACCAAG 
ACACCTCACT ATCATGATGA GTTCAAAATT CAACTACCTG CCGTTCTCTC TCCTGAGCAT 
CACCTTCTGT TCACGTTTTT TCATGTGGAT CTACAGATGA AAATTGAGGC TCCAAAGCCG 
GTGGTAGTGG GATACTCTGT TCTTCCGCTC ATGTTTGTTT GTCAGGTTCA GCGGTTGGAC 
GGCAGCCTAC CTATCGCAAA GGAGCTGTAT TCCAATTATC TACAAGAAAA TGTGAAGGAA 
AGGTTGGAGT ACTTGGATGA TGGCAAAACT GTATTTAAAT TGAGGTCACG ATTATGCTCT 
TCTCTTTATC CGGTTAATGA GAGGATCCGA GATTTCTTTT CAGAATACGA TCGACATGTC 
TTGCGGACCA GTCCACCCTG GGGTACTGAG CTGATGGAGG CTATTAATGC TCTGAAGACG 
GTTGAGCCGT CAGCTATGCT CCAGTTTCTT CAACCTATTC TCAACATGCT GCTTCGTATG 
ATTCGCGATG GAGGAGAAAC GTTGCAGGTT GCTGCATTCC GTTCAATGGT TAACATCATT 
ACCAGGGTAC AAGCAGAGAC ATCTGATGGG GCTGAAAGAA ATCGTTACCT TGTGCATTAT 
GTGGATTATG CATTTAATGA TTTTGGTGGG CGTCATGAGC CTGTGTACCC AGGTCTGTGC 
AGTGTATGGC GCAGCCTTGC TCGAAGCAAG GCGAAAGGCT ACCGTGTCGG ACCTGTGTAT 
GATGATGTTC TTTCCATGGC TTGGGTGTTC CTGGAGCTAA TTGTCAAGTC CATGGCACTT 
GAACAATCCC GCATTTACTC TGAGTCCCTC CCACCTGGAG AGGAGTTACC ACCTTTGCAG 
CTCATTGATG AGGTCTTTAA AAGTATCGTA CAGCTCTATG ATTGTTTACT GACAGAGGTT 
CATGAGCGCT GCAAGAAGGG CATGCTTCTC GCAATGAAGC TGAATAGCAG TATTGCATAC 
TTTTGCTATG ACCTTCTCTC TGTAATTGAG CATCGACAAG TCTTTGAGCT GGTTGCGCTT 
TATTTCAACA AGTTTGCTGG GGTATGTCAA TCTCTTGTGC ACGAATGTAA GCTTAATTTT 
CTGCGCATCG TCTGTGACCA TGATCTCTTT GTTGAGATGC CCGGTCGAGA TCCTACAGAG 
AGGAATTATT TGGCATCACT GTTGATGCAA GAGCTCTTTG TTACTTGGGA TCATGAAGAT 
CTGACTTTAA GATCTAAGGC AGCTCGAATC CTTGTGCAAC TTATGTGCAA GCATGAGTAT 
GATGCACGGT ATCAAACATT GGAGGACAAA ATGTATATTG CCCAACGTTA CTTTCCACTA 
GTTGACCTGA TATTGGACGA AATGCCAGTG TTCTATGGCC TGAGCACTAC AGAGAAGCGT 
GAAGTGCTGG TGTGTGTGCT TCACATTCTG CGATATCTAG ACGATGCATC TCTTGTTAAA 
GCTTGGCAGC ACAACATGGA TAGAACAAAG CTTTTTTTCA AACTCCTGGA AGAATGTCAG 
GAATTGCTGG AGTATAAGAA GGCTGGAGCA GATGGCTTGA TGGGAGCTAT GCCAGCGAAT 
GATCAAGGAG AAGGGCCCCT TCGATACTCC GAGAAGCTCT CACCTGCAGT CAATCAGTTT 
TTGATTGAGG CATCTCGGCA GGATGTCAGA CTCACAAAGA TTTCTCCAAA TGCTCAGTGG 
ATACTTCCAC AAACGAGTCC AGAGATTGGT TCATTCTGGA AAAGAGCAAG CCCTGTAACA 
AATAGTTCAA CTAGCCCAGG TCAGCCGCAT TCGTTGCGAG AGGCACTAGC CCAAGCACAG 
TCTGCGGGAA GAAGTGGAGG CAGGTCTCTT CGTGAGACCT TGCATCCTAT GCTCCGCCAA 
AAGTTGGAGC TATGGGAGGA GAACCTTAGT GCCTCTGCTA TGCTCCAAAT TTTGGAGGTA 
CTTGAGAAGT TTATAAGTGC AGTATCAAGT CAAGCTGTTC TCACTGACTA CATGAGGCTT 
GATTGTATCA CAACTATTTT TATTGGTTTT CTGGGACGCA GCCAGCCGTT GCCATTTTGG 
AAGGCCTTTC TACCTTTCTT CAATAACGTG TTTCGTCGTC ATGGCGCTAC CTTGATGAGT 
AGGGAGAATG ATAGGTTCTT GAAGCAAGTA GCATTTCATC TACTACGGCT CGGAGTTTTC 
CGGAATGAGT CCATACGGAA ACGTTCTGTT GTTGGCCTTC AAATTCTTGT CCGAAATGCA 
TACCACTACT TTCAAGGCCT TTCAAGACTG CGGGTGATGC TTACAATCAC ACTTTCTGAG 
CTCATGTCAG ATGTTCAAGT AACACATCCT AAGATGGATG GATCCTTGGA AGAGAGTGGT 
GAATCTCAAC GTCTTCGTAG GTCCTTGCAA CAGATTGCAC AAGAAAGTAT TAGTGCGGAT 
CTTCTACGGG AATGTGGTCT TCCTGAAGAT TCTCTTACTG CTTTGCCAGA AGGTGTGAAT 
GGCCAACAAT GGAGTTGGGC TGGAGTCGCT GAGCTTTCCA CCTCTCTGCT GAAAGCTGTG 
GATGCTGCTG TAGGACATGC TCTTTTGGGT CCTGAGATTT TAATGAATGA CAAGTATGCA 
ACTGCAGAGG CCTACCATGG TTTGGCGACG GCATATACCA ACGTGCCCGA TCTGCATATA 
ATGTGGCTTC TGTATCTCTG CGAAGTTCAT CAAGGCAACC AGTCTTATGC AGAGGCTGCT 
CAGTGTGCTG TTGCGGTTGC AGGTGTCATC ATGCATGCTA TAGTAGGAAA AGGTGATCAT 
GTTTGGGGAA AAGAGCATGT TGAGGCACTG GGTAATATTT TCCCAATGCT CACTGGTTCC 
CGCTTTGGGG ATGCTGCAAC TGCCGAAATT GAAGGCTATG GGTCATCGAA ATTGACTGTC 
GAATCGGCAG TGAAGTACTT GCAGCTCGCC AATAAACTCT TTGTCCAGGC AGAGCTCTTC 
CATTTCTGTG CCGGCATATT GGAGCTCATA ATTCCCGTCC ATAAGTCAAG GCGTGCCTAT 
AGTCAGCTGT CTAAATGTCA TACTTCATTG ACCAGCATCT ACGAAGCTAT ACTAGAGCAA 
GAGTCGAGCC CTATCCCTTT CACGGATGCA ACATATTATC GTGTGGGATT TTATGGGAAA 
AGCTTTGGTA GTTTGCACTG CAAGGAGTAT GTGTACAGGG AACCACGGGA CGTGCGGTTA 
GGGGACATTA TGAAAAATTT AGGAAATATT TATGAGCCTA CAGTAATTGA AGGAAAGCAG 
AACTTGCATA TTATCCCCGA TTCTCGTCAA GTGAATCCAG AGGATCTTCA AGCTGGCATT 
TGTTACTTGC AAATTACATC TGTCGATCCT GTTACAGAGG ACGAGGATTT GGAAAGCAGG 
CGAGAAAGAC AATCCGGGAG GTCCTCGTCA ACATTCAGTG CACGAGTCTT CGATCGATTT 
TTATTTGACA CTCCTTTCAC AAAGAATGGA AGATCCCAAG GAGGTTTGGA AGATCAGTGG 
AAGAGGCGTA CGGTTTTGTG GACTGAAGGG CCTTTTCCAT CTCTTGTAAA TCGTTTAATG 
GTTGTGAAGT CTGAGTCGCG AGAATTTTCG CCAATCGAAA ACGCCATAGG AATGATTGAG 
ACTCGTACAG CAGCTTTAGC AGGAGAGCTC GATGATCAAC GTATAAATGA GGGAGATCCT 
CTGCCCCGTT TACAAGGCCT GCAAAGAATC TTGCAAGGAA GTGTTGCAGT CCAGGTAAAT 
AGTGGAGTGC TAGGTGTCTG CACAGCTTTC TTGTCTGGGG AACCTTCAAC ACGATTGCGC 
TCACAAGAAC TACAGCAGCT GATAGCAGCA CTCTTGGAGT TTATGGCTGT GTGCAAGAAA 
GCAATTCGAG TACATGCACG ACTGATTGGA GAGGAGGACC AAGACTTCCA CTCGCAGCTT 
GTGATTGGAT TCCAATCACT CACAGCAGAG CTATCCCACT ATATTCCCGC CATCTTGTCA 
GATGTAACCA TACTTGGGAA ACACAGCAAT GTGCTCAAGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024369344.1
CDS	340..6264
Translation	MHLVAGAPIWSVESVKEGKGSRFDVSDSVGQCRVGSHDRRDEEETWGLNAETESSAFWELSFETRAVVDSGSIKKQGMDEIALSGQRFKRIPHPYIGQSTDLESLLNGNGDPFPHFKELLQSYRAEWVKDEFKYGHYEAVPPPALEHQIFEGPDTDIQTEFRLSNVRLGRNGDVTDDEDPQTPRRLSRRFSVRSSGRFSMRSTTSDAPEALHTNISKHYGVAPLPPYEPVYNWRATRASVLGQRTSELPSAISSSSGLKVSVKVVSLNVQAGLVEPMYGTLCLYHREKREKLSENFHFRCLPSEFQDCVTWQEGGNCLRRAIFSLEAASPAICLLIQLEKHVTEEGGVTAHVYSRKEPVHLTEREKQKLQVWARVMPFREPFAWAVVALFDANITGGVGGFSSSPSSPLPPGLFGPSLMEASLDSDGRLIADAKTESSSVPVLVDVQSLRRVKENYTEEMLQDPKYKVHKPVKAILRLEIERLSTEDHDQDSIFECGSFGRGSTDGDSRLTVGPSFSGRFMQGLGMTKVTHNGRQKWSPGRKHRYLHSGSVGSIEFGKFDFRAIEFRSGSKVEVLPQLLHCLYVYPQSVNLSRKRNLFVRVELRKDDVDIRKSPLEALYPRDADSSMQKYAHSQIDANTKTPHYHDEFKIQLPAVLSPEHHLLFTFFHVDLQMKIEAPKPVVVGYSVLPLMFVCQVQRLDGSLPIAKELYSNYLQENVKERLEYLDDGKTVFKLRSRLCSSLYPVNERIRDFFSEYDRHVLRTSPPWGTELMEAINALKTVEPSAMLQFLQPILNMLLRMIRDGGETLQVAAFRSMVNIITRVQAETSDGAERNRYLVHYVDYAFNDFGGRHEPVYPGLCSVWRSLARSKAKGYRVGPVYDDVLSMAWVFLELIVKSMALEQSRIYSESLPPGEELPPLQLIDEVFKSIVQLYDCLLTEVHERCKKGMLLAMKLNSSIAYFCYDLLSVIEHRQVFELVALYFNKFAGVCQSLVHECKLNFLRIVCDHDLFVEMPGRDPTERNYLASLLMQELFVTWDHEDLTLRSKAARILVQLMCKHEYDARYQTLEDKMYIAQRYFPLVDLILDEMPVFYGLSTTEKREVLVCVLHILRYLDDASLVKAWQHNMDRTKLFFKLLEECQELLEYKKAGADGLMGAMPANDQGEGPLRYSEKLSPAVNQFLIEASRQDVRLTKISPNAQWILPQTSPEIGSFWKRASPVTNSSTSPGQPHSLREALAQAQSAGRSGGRSLRETLHPMLRQKLELWEENLSASAMLQILEVLEKFISAVSSQAVLTDYMRLDCITTIFIGFLGRSQPLPFWKAFLPFFNNVFRRHGATLMSRENDRFLKQVAFHLLRLGVFRNESIRKRSVVGLQILVRNAYHYFQGLSRLRVMLTITLSELMSDVQVTHPKMDGSLEESGESQRLRRSLQQIAQESISADLLRECGLPEDSLTALPEGVNGQQWSWAGVAELSTSLLKAVDAAVGHALLGPEILMNDKYATAEAYHGLATAYTNVPDLHIMWLLYLCEVHQGNQSYAEAAQCAVAVAGVIMHAIVGKGDHVWGKEHVEALGNIFPMLTGSRFGDAATAEIEGYGSSKLTVESAVKYLQLANKLFVQAELFHFCAGILELIIPVHKSRRAYSQLSKCHTSLTSIYEAILEQESSPIPFTDATYYRVGFYGKSFGSLHCKEYVYREPRDVRLGDIMKNLGNIYEPTVIEGKQNLHIIPDSRQVNPEDLQAGICYLQITSVDPVTEDEDLESRRERQSGRSSSTFSARVFDRFLFDTPFTKNGRSQGGLEDQWKRRTVLWTEGPFPSLVNRLMVVKSESREFSPIENAIGMIETRTAALAGELDDQRINEGDPLPRLQGLQRILQGSVAVQVNSGVLGVCTAFLSGEPSTRLRSQELQQLIAALLEFMAVCKKAIRVHARLIGEEDQDFHSQLVIGFQSLTAELSHYIPAILSDVTILGKHSNVLKL

Target ORF information:

RefSeq Version	XM_024513576.1
Organism	Physcomitrella patens
Definition	Physcomitrella patens guanine nucleotide exchange factor SPIKE 1-like (LOC112279271), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024513576.1

ORF Insert Sequence:

ATGCATTTGG TCGCTGGAGC GCCTATATGG TCGGTGGAAA GCGTCAAGGA GGGAAAAGGA 
TCCAGGTTCG ACGTGTCGGA CTCAGTAGGA CAATGTAGGG TAGGTAGTCA TGATCGGAGG 
GACGAGGAGG AGACATGGGG ACTCAATGCA GAGACAGAAA GCTCTGCATT CTGGGAGCTG 
AGCTTCGAGA CTAGAGCTGT CGTCGACAGC GGATCAATTA AGAAGCAGGG GATGGATGAA 
ATTGCCTTGA GCGGGCAGCG GTTCAAACGT ATCCCACACC CGTACATCGG GCAGTCAACA 
GATCTTGAGT CCTTGCTGAA TGGGAATGGA GACCCTTTCC CACATTTCAA AGAGCTTCTT 
CAAAGCTACA GGGCTGAATG GGTGAAGGAT GAGTTCAAAT ACGGTCATTA TGAAGCTGTG 
CCACCGCCAG CCTTAGAACA TCAGATTTTT GAAGGACCCG ACACTGACAT ACAGACAGAG 
TTTCGGCTTT CTAACGTCAG ACTCGGCAGA AATGGTGATG TTACTGATGA TGAAGATCCT 
CAAACTCCAC GACGGCTTTC AAGACGTTTT TCTGTACGGT CATCTGGTCG TTTCTCTATG 
AGATCTACGA CTAGTGATGC ACCTGAAGCA TTGCACACAA ATATCTCAAA GCATTATGGG 
GTTGCTCCGT TACCTCCTTA TGAGCCCGTT TATAATTGGC GAGCAACAAG GGCATCTGTA 
CTTGGACAAA GAACTTCGGA ACTTCCATCA GCAATATCAT CTAGTAGCGG GCTGAAGGTT 
TCTGTGAAGG TTGTCTCTTT GAATGTTCAG GCCGGACTTG TCGAACCCAT GTACGGTACC 
TTGTGTCTCT ATCATCGAGA GAAGCGGGAG AAATTATCAG AAAATTTTCA CTTCCGATGT 
TTACCATCAG AGTTCCAAGA TTGTGTAACT TGGCAGGAGG GTGGTAATTG TCTGAGAAGA 
GCTATATTTT CACTGGAGGC AGCGTCGCCA GCAATATGTT TGTTGATCCA GCTTGAAAAG 
CATGTGACTG AGGAAGGAGG AGTAACGGCT CATGTTTATT CTCGAAAAGA ACCTGTACAC 
TTGACCGAGA GAGAAAAGCA GAAGTTACAG GTCTGGGCGC GCGTGATGCC ATTCAGGGAG 
CCTTTCGCGT GGGCAGTAGT TGCTCTGTTT GATGCAAATA TAACAGGAGG TGTAGGAGGA 
TTCTCATCTT CTCCAAGCAG CCCACTTCCA CCAGGACTTT TTGGACCATC TTTGATGGAA 
GCATCCTTGG ATTCTGATGG ACGACTTATA GCTGATGCTA AGACAGAATC ATCTAGTGTG 
CCAGTTTTGG TTGATGTTCA GAGCCTTCGC CGGGTCAAAG AAAATTACAC GGAGGAAATG 
CTTCAGGATC CCAAGTACAA AGTGCACAAA CCCGTGAAGG CCATCCTTCG TCTCGAGATA 
GAGAGGTTAT CTACTGAAGA TCATGACCAA GATTCCATAT TTGAGTGTGG TAGTTTTGGT 
CGTGGGTCCA CTGATGGTGA TTCCCGCTTG ACAGTAGGAC CCTCTTTCTC AGGGCGTTTC 
ATGCAGGGGC TGGGCATGAC GAAGGTTACG CATAATGGGC GGCAGAAATG GAGCCCTGGA 
AGAAAACATC GCTATCTGCA TTCCGGCAGT GTTGGCAGCA TTGAATTTGG AAAGTTTGAC 
TTCCGGGCTA TTGAGTTTCG ATCGGGTTCG AAAGTTGAGG TTTTACCTCA GCTATTGCAC 
TGCCTGTATG TATATCCTCA ATCAGTCAAT TTAAGTCGAA AACGCAATCT TTTTGTGCGA 
GTGGAGCTTC GAAAGGATGA TGTAGACATT CGCAAATCTC CTCTCGAGGC CCTTTATCCG 
AGGGATGCTG ACAGCTCTAT GCAGAAATAT GCACACTCTC AGATCGATGC AAACACCAAG 
ACACCTCACT ATCATGATGA GTTCAAAATT CAACTACCTG CCGTTCTCTC TCCTGAGCAT 
CACCTTCTGT TCACGTTTTT TCATGTGGAT CTACAGATGA AAATTGAGGC TCCAAAGCCG 
GTGGTAGTGG GATACTCTGT TCTTCCGCTC ATGTTTGTTT GTCAGGTTCA GCGGTTGGAC 
GGCAGCCTAC CTATCGCAAA GGAGCTGTAT TCCAATTATC TACAAGAAAA TGTGAAGGAA 
AGGTTGGAGT ACTTGGATGA TGGCAAAACT GTATTTAAAT TGAGGTCACG ATTATGCTCT 
TCTCTTTATC CGGTTAATGA GAGGATCCGA GATTTCTTTT CAGAATACGA TCGACATGTC 
TTGCGGACCA GTCCACCCTG GGGTACTGAG CTGATGGAGG CTATTAATGC TCTGAAGACG 
GTTGAGCCGT CAGCTATGCT CCAGTTTCTT CAACCTATTC TCAACATGCT GCTTCGTATG 
ATTCGCGATG GAGGAGAAAC GTTGCAGGTT GCTGCATTCC GTTCAATGGT TAACATCATT 
ACCAGGGTAC AAGCAGAGAC ATCTGATGGG GCTGAAAGAA ATCGTTACCT TGTGCATTAT 
GTGGATTATG CATTTAATGA TTTTGGTGGG CGTCATGAGC CTGTGTACCC AGGTCTGTGC 
AGTGTATGGC GCAGCCTTGC TCGAAGCAAG GCGAAAGGCT ACCGTGTCGG ACCTGTGTAT 
GATGATGTTC TTTCCATGGC TTGGGTGTTC CTGGAGCTAA TTGTCAAGTC CATGGCACTT 
GAACAATCCC GCATTTACTC TGAGTCCCTC CCACCTGGAG AGGAGTTACC ACCTTTGCAG 
CTCATTGATG AGGTCTTTAA AAGTATCGTA CAGCTCTATG ATTGTTTACT GACAGAGGTT 
CATGAGCGCT GCAAGAAGGG CATGCTTCTC GCAATGAAGC TGAATAGCAG TATTGCATAC 
TTTTGCTATG ACCTTCTCTC TGTAATTGAG CATCGACAAG TCTTTGAGCT GGTTGCGCTT 
TATTTCAACA AGTTTGCTGG GGTATGTCAA TCTCTTGTGC ACGAATGTAA GCTTAATTTT 
CTGCGCATCG TCTGTGACCA TGATCTCTTT GTTGAGATGC CCGGTCGAGA TCCTACAGAG 
AGGAATTATT TGGCATCACT GTTGATGCAA GAGCTCTTTG TTACTTGGGA TCATGAAGAT 
CTGACTTTAA GATCTAAGGC AGCTCGAATC CTTGTGCAAC TTATGTGCAA GCATGAGTAT 
GATGCACGGT ATCAAACATT GGAGGACAAA ATGTATATTG CCCAACGTTA CTTTCCACTA 
GTTGACCTGA TATTGGACGA AATGCCAGTG TTCTATGGCC TGAGCACTAC AGAGAAGCGT 
GAAGTGCTGG TGTGTGTGCT TCACATTCTG CGATATCTAG ACGATGCATC TCTTGTTAAA 
GCTTGGCAGC ACAACATGGA TAGAACAAAG CTTTTTTTCA AACTCCTGGA AGAATGTCAG 
GAATTGCTGG AGTATAAGAA GGCTGGAGCA GATGGCTTGA TGGGAGCTAT GCCAGCGAAT 
GATCAAGGAG AAGGGCCCCT TCGATACTCC GAGAAGCTCT CACCTGCAGT CAATCAGTTT 
TTGATTGAGG CATCTCGGCA GGATGTCAGA CTCACAAAGA TTTCTCCAAA TGCTCAGTGG 
ATACTTCCAC AAACGAGTCC AGAGATTGGT TCATTCTGGA AAAGAGCAAG CCCTGTAACA 
AATAGTTCAA CTAGCCCAGG TCAGCCGCAT TCGTTGCGAG AGGCACTAGC CCAAGCACAG 
TCTGCGGGAA GAAGTGGAGG CAGGTCTCTT CGTGAGACCT TGCATCCTAT GCTCCGCCAA 
AAGTTGGAGC TATGGGAGGA GAACCTTAGT GCCTCTGCTA TGCTCCAAAT TTTGGAGGTA 
CTTGAGAAGT TTATAAGTGC AGTATCAAGT CAAGCTGTTC TCACTGACTA CATGAGGCTT 
GATTGTATCA CAACTATTTT TATTGGTTTT CTGGGACGCA GCCAGCCGTT GCCATTTTGG 
AAGGCCTTTC TACCTTTCTT CAATAACGTG TTTCGTCGTC ATGGCGCTAC CTTGATGAGT 
AGGGAGAATG ATAGGTTCTT GAAGCAAGTA GCATTTCATC TACTACGGCT CGGAGTTTTC 
CGGAATGAGT CCATACGGAA ACGTTCTGTT GTTGGCCTTC AAATTCTTGT CCGAAATGCA 
TACCACTACT TTCAAGGCCT TTCAAGACTG CGGGTGATGC TTACAATCAC ACTTTCTGAG 
CTCATGTCAG ATGTTCAAGT AACACATCCT AAGATGGATG GATCCTTGGA AGAGAGTGGT 
GAATCTCAAC GTCTTCGTAG GTCCTTGCAA CAGATTGCAC AAGAAAGTAT TAGTGCGGAT 
CTTCTACGGG AATGTGGTCT TCCTGAAGAT TCTCTTACTG CTTTGCCAGA AGGTGTGAAT 
GGCCAACAAT GGAGTTGGGC TGGAGTCGCT GAGCTTTCCA CCTCTCTGCT GAAAGCTGTG 
GATGCTGCTG TAGGACATGC TCTTTTGGGT CCTGAGATTT TAATGAATGA CAAGTATGCA 
ACTGCAGAGG CCTACCATGG TTTGGCGACG GCATATACCA ACGTGCCCGA TCTGCATATA 
ATGTGGCTTC TGTATCTCTG CGAAGTTCAT CAAGGCAACC AGTCTTATGC AGAGGCTGCT 
CAGTGTGCTG TTGCGGTTGC AGGTGTCATC ATGCATGCTA TAGTAGGAAA AGGTGATCAT 
GTTTGGGGAA AAGAGCATGT TGAGGCACTG GGTAATATTT TCCCAATGCT CACTGGTTCC 
CGCTTTGGGG ATGCTGCAAC TGCCGAAATT GAAGGCTATG GGTCATCGAA ATTGACTGTC 
GAATCGGCAG TGAAGTACTT GCAGCTCGCC AATAAACTCT TTGTCCAGGC AGAGCTCTTC 
CATTTCTGTG CCGGCATATT GGAGCTCATA ATTCCCGTCC ATAAGTCAAG GCGTGCCTAT 
AGTCAGCTGT CTAAATGTCA TACTTCATTG ACCAGCATCT ACGAAGCTAT ACTAGAGCAA 
GAGTCGAGCC CTATCCCTTT CACGGATGCA ACATATTATC GTGTGGGATT TTATGGGAAA 
AGCTTTGGTA GTTTGCACTG CAAGGAGTAT GTGTACAGGG AACCACGGGA CGTGCGGTTA 
GGGGACATTA TGAAAAATTT AGGAAATATT TATGAGCCTA CAGTAATTGA AGGAAAGCAG 
AACTTGCATA TTATCCCCGA TTCTCGTCAA GTGAATCCAG AGGATCTTCA AGCTGGCATT 
TGTTACTTGC AAATTACATC TGTCGATCCT GTTACAGAGG ACGAGGATTT GGAAAGCAGG 
CGAGAAAGAC AATCCGGGAG GTCCTCGTCA ACATTCAGTG CACGAGTCTT CGATCGATTT 
TTATTTGACA CTCCTTTCAC AAAGAATGGA AGATCCCAAG GAGGTTTGGA AGATCAGTGG 
AAGAGGCGTA CGGTTTTGTG GACTGAAGGG CCTTTTCCAT CTCTTGTAAA TCGTTTAATG 
GTTGTGAAGT CTGAGTCGCG AGAATTTTCG CCAATCGAAA ACGCCATAGG AATGATTGAG 
ACTCGTACAG CAGCTTTAGC AGGAGAGCTC GATGATCAAC GTATAAATGA GGGAGATCCT 
CTGCCCCGTT TACAAGGCCT GCAAAGAATC TTGCAAGGAA GTGTTGCAGT CCAGGTAAAT 
AGTGGAGTGC TAGGTGTCTG CACAGCTTTC TTGTCTGGGG AACCTTCAAC ACGATTGCGC 
TCACAAGAAC TACAGCAGCT GATAGCAGCA CTCTTGGAGT TTATGGCTGT GTGCAAGAAA 
GCAATTCGAG TACATGCACG ACTGATTGGA GAGGAGGACC AAGACTTCCA CTCGCAGCTT 
GTGATTGGAT TCCAATCACT CACAGCAGAG CTATCCCACT ATATTCCCGC CATCTTGTCA 
GATGTAACCA TACTTGGGAA ACACAGCAAT GTGCTCAAGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC112279271 ( XM_024513552.1 ) cDNA ORF clone, -> XP_024369320.1 Physcomitrella patens guanine nucleotide exchange factor SPIKE 1-like (LOC112279271), transcript variant X3, mRNA.

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CloneID	OPn41280
Clone ID Related Accession (Same CDS sequence)	XM_024513560.1 , XM_024513543.1 , XM_024513534.1 , XM_024513569.1 , XM_024513576.1 , XM_024513552.1 , XM_024513585.1
Accession Version	XM_024513552.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Physcomitrella patens
Product	guanine nucleotide exchange factor SPIKE 1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037253.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physcomitrella patens Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATTTGG TCGCTGGAGC GCCTATATGG TCGGTGGAAA GCGTCAAGGA GGGAAAAGGA 
TCCAGGTTCG ACGTGTCGGA CTCAGTAGGA CAATGTAGGG TAGGTAGTCA TGATCGGAGG 
GACGAGGAGG AGACATGGGG ACTCAATGCA GAGACAGAAA GCTCTGCATT CTGGGAGCTG 
AGCTTCGAGA CTAGAGCTGT CGTCGACAGC GGATCAATTA AGAAGCAGGG GATGGATGAA 
ATTGCCTTGA GCGGGCAGCG GTTCAAACGT ATCCCACACC CGTACATCGG GCAGTCAACA 
GATCTTGAGT CCTTGCTGAA TGGGAATGGA GACCCTTTCC CACATTTCAA AGAGCTTCTT 
CAAAGCTACA GGGCTGAATG GGTGAAGGAT GAGTTCAAAT ACGGTCATTA TGAAGCTGTG 
CCACCGCCAG CCTTAGAACA TCAGATTTTT GAAGGACCCG ACACTGACAT ACAGACAGAG 
TTTCGGCTTT CTAACGTCAG ACTCGGCAGA AATGGTGATG TTACTGATGA TGAAGATCCT 
CAAACTCCAC GACGGCTTTC AAGACGTTTT TCTGTACGGT CATCTGGTCG TTTCTCTATG 
AGATCTACGA CTAGTGATGC ACCTGAAGCA TTGCACACAA ATATCTCAAA GCATTATGGG 
GTTGCTCCGT TACCTCCTTA TGAGCCCGTT TATAATTGGC GAGCAACAAG GGCATCTGTA 
CTTGGACAAA GAACTTCGGA ACTTCCATCA GCAATATCAT CTAGTAGCGG GCTGAAGGTT 
TCTGTGAAGG TTGTCTCTTT GAATGTTCAG GCCGGACTTG TCGAACCCAT GTACGGTACC 
TTGTGTCTCT ATCATCGAGA GAAGCGGGAG AAATTATCAG AAAATTTTCA CTTCCGATGT 
TTACCATCAG AGTTCCAAGA TTGTGTAACT TGGCAGGAGG GTGGTAATTG TCTGAGAAGA 
GCTATATTTT CACTGGAGGC AGCGTCGCCA GCAATATGTT TGTTGATCCA GCTTGAAAAG 
CATGTGACTG AGGAAGGAGG AGTAACGGCT CATGTTTATT CTCGAAAAGA ACCTGTACAC 
TTGACCGAGA GAGAAAAGCA GAAGTTACAG GTCTGGGCGC GCGTGATGCC ATTCAGGGAG 
CCTTTCGCGT GGGCAGTAGT TGCTCTGTTT GATGCAAATA TAACAGGAGG TGTAGGAGGA 
TTCTCATCTT CTCCAAGCAG CCCACTTCCA CCAGGACTTT TTGGACCATC TTTGATGGAA 
GCATCCTTGG ATTCTGATGG ACGACTTATA GCTGATGCTA AGACAGAATC ATCTAGTGTG 
CCAGTTTTGG TTGATGTTCA GAGCCTTCGC CGGGTCAAAG AAAATTACAC GGAGGAAATG 
CTTCAGGATC CCAAGTACAA AGTGCACAAA CCCGTGAAGG CCATCCTTCG TCTCGAGATA 
GAGAGGTTAT CTACTGAAGA TCATGACCAA GATTCCATAT TTGAGTGTGG TAGTTTTGGT 
CGTGGGTCCA CTGATGGTGA TTCCCGCTTG ACAGTAGGAC CCTCTTTCTC AGGGCGTTTC 
ATGCAGGGGC TGGGCATGAC GAAGGTTACG CATAATGGGC GGCAGAAATG GAGCCCTGGA 
AGAAAACATC GCTATCTGCA TTCCGGCAGT GTTGGCAGCA TTGAATTTGG AAAGTTTGAC 
TTCCGGGCTA TTGAGTTTCG ATCGGGTTCG AAAGTTGAGG TTTTACCTCA GCTATTGCAC 
TGCCTGTATG TATATCCTCA ATCAGTCAAT TTAAGTCGAA AACGCAATCT TTTTGTGCGA 
GTGGAGCTTC GAAAGGATGA TGTAGACATT CGCAAATCTC CTCTCGAGGC CCTTTATCCG 
AGGGATGCTG ACAGCTCTAT GCAGAAATAT GCACACTCTC AGATCGATGC AAACACCAAG 
ACACCTCACT ATCATGATGA GTTCAAAATT CAACTACCTG CCGTTCTCTC TCCTGAGCAT 
CACCTTCTGT TCACGTTTTT TCATGTGGAT CTACAGATGA AAATTGAGGC TCCAAAGCCG 
GTGGTAGTGG GATACTCTGT TCTTCCGCTC ATGTTTGTTT GTCAGGTTCA GCGGTTGGAC 
GGCAGCCTAC CTATCGCAAA GGAGCTGTAT TCCAATTATC TACAAGAAAA TGTGAAGGAA 
AGGTTGGAGT ACTTGGATGA TGGCAAAACT GTATTTAAAT TGAGGTCACG ATTATGCTCT 
TCTCTTTATC CGGTTAATGA GAGGATCCGA GATTTCTTTT CAGAATACGA TCGACATGTC 
TTGCGGACCA GTCCACCCTG GGGTACTGAG CTGATGGAGG CTATTAATGC TCTGAAGACG 
GTTGAGCCGT CAGCTATGCT CCAGTTTCTT CAACCTATTC TCAACATGCT GCTTCGTATG 
ATTCGCGATG GAGGAGAAAC GTTGCAGGTT GCTGCATTCC GTTCAATGGT TAACATCATT 
ACCAGGGTAC AAGCAGAGAC ATCTGATGGG GCTGAAAGAA ATCGTTACCT TGTGCATTAT 
GTGGATTATG CATTTAATGA TTTTGGTGGG CGTCATGAGC CTGTGTACCC AGGTCTGTGC 
AGTGTATGGC GCAGCCTTGC TCGAAGCAAG GCGAAAGGCT ACCGTGTCGG ACCTGTGTAT 
GATGATGTTC TTTCCATGGC TTGGGTGTTC CTGGAGCTAA TTGTCAAGTC CATGGCACTT 
GAACAATCCC GCATTTACTC TGAGTCCCTC CCACCTGGAG AGGAGTTACC ACCTTTGCAG 
CTCATTGATG AGGTCTTTAA AAGTATCGTA CAGCTCTATG ATTGTTTACT GACAGAGGTT 
CATGAGCGCT GCAAGAAGGG CATGCTTCTC GCAATGAAGC TGAATAGCAG TATTGCATAC 
TTTTGCTATG ACCTTCTCTC TGTAATTGAG CATCGACAAG TCTTTGAGCT GGTTGCGCTT 
TATTTCAACA AGTTTGCTGG GGTATGTCAA TCTCTTGTGC ACGAATGTAA GCTTAATTTT 
CTGCGCATCG TCTGTGACCA TGATCTCTTT GTTGAGATGC CCGGTCGAGA TCCTACAGAG 
AGGAATTATT TGGCATCACT GTTGATGCAA GAGCTCTTTG TTACTTGGGA TCATGAAGAT 
CTGACTTTAA GATCTAAGGC AGCTCGAATC CTTGTGCAAC TTATGTGCAA GCATGAGTAT 
GATGCACGGT ATCAAACATT GGAGGACAAA ATGTATATTG CCCAACGTTA CTTTCCACTA 
GTTGACCTGA TATTGGACGA AATGCCAGTG TTCTATGGCC TGAGCACTAC AGAGAAGCGT 
GAAGTGCTGG TGTGTGTGCT TCACATTCTG CGATATCTAG ACGATGCATC TCTTGTTAAA 
GCTTGGCAGC ACAACATGGA TAGAACAAAG CTTTTTTTCA AACTCCTGGA AGAATGTCAG 
GAATTGCTGG AGTATAAGAA GGCTGGAGCA GATGGCTTGA TGGGAGCTAT GCCAGCGAAT 
GATCAAGGAG AAGGGCCCCT TCGATACTCC GAGAAGCTCT CACCTGCAGT CAATCAGTTT 
TTGATTGAGG CATCTCGGCA GGATGTCAGA CTCACAAAGA TTTCTCCAAA TGCTCAGTGG 
ATACTTCCAC AAACGAGTCC AGAGATTGGT TCATTCTGGA AAAGAGCAAG CCCTGTAACA 
AATAGTTCAA CTAGCCCAGG TCAGCCGCAT TCGTTGCGAG AGGCACTAGC CCAAGCACAG 
TCTGCGGGAA GAAGTGGAGG CAGGTCTCTT CGTGAGACCT TGCATCCTAT GCTCCGCCAA 
AAGTTGGAGC TATGGGAGGA GAACCTTAGT GCCTCTGCTA TGCTCCAAAT TTTGGAGGTA 
CTTGAGAAGT TTATAAGTGC AGTATCAAGT CAAGCTGTTC TCACTGACTA CATGAGGCTT 
GATTGTATCA CAACTATTTT TATTGGTTTT CTGGGACGCA GCCAGCCGTT GCCATTTTGG 
AAGGCCTTTC TACCTTTCTT CAATAACGTG TTTCGTCGTC ATGGCGCTAC CTTGATGAGT 
AGGGAGAATG ATAGGTTCTT GAAGCAAGTA GCATTTCATC TACTACGGCT CGGAGTTTTC 
CGGAATGAGT CCATACGGAA ACGTTCTGTT GTTGGCCTTC AAATTCTTGT CCGAAATGCA 
TACCACTACT TTCAAGGCCT TTCAAGACTG CGGGTGATGC TTACAATCAC ACTTTCTGAG 
CTCATGTCAG ATGTTCAAGT AACACATCCT AAGATGGATG GATCCTTGGA AGAGAGTGGT 
GAATCTCAAC GTCTTCGTAG GTCCTTGCAA CAGATTGCAC AAGAAAGTAT TAGTGCGGAT 
CTTCTACGGG AATGTGGTCT TCCTGAAGAT TCTCTTACTG CTTTGCCAGA AGGTGTGAAT 
GGCCAACAAT GGAGTTGGGC TGGAGTCGCT GAGCTTTCCA CCTCTCTGCT GAAAGCTGTG 
GATGCTGCTG TAGGACATGC TCTTTTGGGT CCTGAGATTT TAATGAATGA CAAGTATGCA 
ACTGCAGAGG CCTACCATGG TTTGGCGACG GCATATACCA ACGTGCCCGA TCTGCATATA 
ATGTGGCTTC TGTATCTCTG CGAAGTTCAT CAAGGCAACC AGTCTTATGC AGAGGCTGCT 
CAGTGTGCTG TTGCGGTTGC AGGTGTCATC ATGCATGCTA TAGTAGGAAA AGGTGATCAT 
GTTTGGGGAA AAGAGCATGT TGAGGCACTG GGTAATATTT TCCCAATGCT CACTGGTTCC 
CGCTTTGGGG ATGCTGCAAC TGCCGAAATT GAAGGCTATG GGTCATCGAA ATTGACTGTC 
GAATCGGCAG TGAAGTACTT GCAGCTCGCC AATAAACTCT TTGTCCAGGC AGAGCTCTTC 
CATTTCTGTG CCGGCATATT GGAGCTCATA ATTCCCGTCC ATAAGTCAAG GCGTGCCTAT 
AGTCAGCTGT CTAAATGTCA TACTTCATTG ACCAGCATCT ACGAAGCTAT ACTAGAGCAA 
GAGTCGAGCC CTATCCCTTT CACGGATGCA ACATATTATC GTGTGGGATT TTATGGGAAA 
AGCTTTGGTA GTTTGCACTG CAAGGAGTAT GTGTACAGGG AACCACGGGA CGTGCGGTTA 
GGGGACATTA TGAAAAATTT AGGAAATATT TATGAGCCTA CAGTAATTGA AGGAAAGCAG 
AACTTGCATA TTATCCCCGA TTCTCGTCAA GTGAATCCAG AGGATCTTCA AGCTGGCATT 
TGTTACTTGC AAATTACATC TGTCGATCCT GTTACAGAGG ACGAGGATTT GGAAAGCAGG 
CGAGAAAGAC AATCCGGGAG GTCCTCGTCA ACATTCAGTG CACGAGTCTT CGATCGATTT 
TTATTTGACA CTCCTTTCAC AAAGAATGGA AGATCCCAAG GAGGTTTGGA AGATCAGTGG 
AAGAGGCGTA CGGTTTTGTG GACTGAAGGG CCTTTTCCAT CTCTTGTAAA TCGTTTAATG 
GTTGTGAAGT CTGAGTCGCG AGAATTTTCG CCAATCGAAA ACGCCATAGG AATGATTGAG 
ACTCGTACAG CAGCTTTAGC AGGAGAGCTC GATGATCAAC GTATAAATGA GGGAGATCCT 
CTGCCCCGTT TACAAGGCCT GCAAAGAATC TTGCAAGGAA GTGTTGCAGT CCAGGTAAAT 
AGTGGAGTGC TAGGTGTCTG CACAGCTTTC TTGTCTGGGG AACCTTCAAC ACGATTGCGC 
TCACAAGAAC TACAGCAGCT GATAGCAGCA CTCTTGGAGT TTATGGCTGT GTGCAAGAAA 
GCAATTCGAG TACATGCACG ACTGATTGGA GAGGAGGACC AAGACTTCCA CTCGCAGCTT 
GTGATTGGAT TCCAATCACT CACAGCAGAG CTATCCCACT ATATTCCCGC CATCTTGTCA 
GATGTAACCA TACTTGGGAA ACACAGCAAT GTGCTCAAGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024369320.1
CDS	537..6461
Translation	MHLVAGAPIWSVESVKEGKGSRFDVSDSVGQCRVGSHDRRDEEETWGLNAETESSAFWELSFETRAVVDSGSIKKQGMDEIALSGQRFKRIPHPYIGQSTDLESLLNGNGDPFPHFKELLQSYRAEWVKDEFKYGHYEAVPPPALEHQIFEGPDTDIQTEFRLSNVRLGRNGDVTDDEDPQTPRRLSRRFSVRSSGRFSMRSTTSDAPEALHTNISKHYGVAPLPPYEPVYNWRATRASVLGQRTSELPSAISSSSGLKVSVKVVSLNVQAGLVEPMYGTLCLYHREKREKLSENFHFRCLPSEFQDCVTWQEGGNCLRRAIFSLEAASPAICLLIQLEKHVTEEGGVTAHVYSRKEPVHLTEREKQKLQVWARVMPFREPFAWAVVALFDANITGGVGGFSSSPSSPLPPGLFGPSLMEASLDSDGRLIADAKTESSSVPVLVDVQSLRRVKENYTEEMLQDPKYKVHKPVKAILRLEIERLSTEDHDQDSIFECGSFGRGSTDGDSRLTVGPSFSGRFMQGLGMTKVTHNGRQKWSPGRKHRYLHSGSVGSIEFGKFDFRAIEFRSGSKVEVLPQLLHCLYVYPQSVNLSRKRNLFVRVELRKDDVDIRKSPLEALYPRDADSSMQKYAHSQIDANTKTPHYHDEFKIQLPAVLSPEHHLLFTFFHVDLQMKIEAPKPVVVGYSVLPLMFVCQVQRLDGSLPIAKELYSNYLQENVKERLEYLDDGKTVFKLRSRLCSSLYPVNERIRDFFSEYDRHVLRTSPPWGTELMEAINALKTVEPSAMLQFLQPILNMLLRMIRDGGETLQVAAFRSMVNIITRVQAETSDGAERNRYLVHYVDYAFNDFGGRHEPVYPGLCSVWRSLARSKAKGYRVGPVYDDVLSMAWVFLELIVKSMALEQSRIYSESLPPGEELPPLQLIDEVFKSIVQLYDCLLTEVHERCKKGMLLAMKLNSSIAYFCYDLLSVIEHRQVFELVALYFNKFAGVCQSLVHECKLNFLRIVCDHDLFVEMPGRDPTERNYLASLLMQELFVTWDHEDLTLRSKAARILVQLMCKHEYDARYQTLEDKMYIAQRYFPLVDLILDEMPVFYGLSTTEKREVLVCVLHILRYLDDASLVKAWQHNMDRTKLFFKLLEECQELLEYKKAGADGLMGAMPANDQGEGPLRYSEKLSPAVNQFLIEASRQDVRLTKISPNAQWILPQTSPEIGSFWKRASPVTNSSTSPGQPHSLREALAQAQSAGRSGGRSLRETLHPMLRQKLELWEENLSASAMLQILEVLEKFISAVSSQAVLTDYMRLDCITTIFIGFLGRSQPLPFWKAFLPFFNNVFRRHGATLMSRENDRFLKQVAFHLLRLGVFRNESIRKRSVVGLQILVRNAYHYFQGLSRLRVMLTITLSELMSDVQVTHPKMDGSLEESGESQRLRRSLQQIAQESISADLLRECGLPEDSLTALPEGVNGQQWSWAGVAELSTSLLKAVDAAVGHALLGPEILMNDKYATAEAYHGLATAYTNVPDLHIMWLLYLCEVHQGNQSYAEAAQCAVAVAGVIMHAIVGKGDHVWGKEHVEALGNIFPMLTGSRFGDAATAEIEGYGSSKLTVESAVKYLQLANKLFVQAELFHFCAGILELIIPVHKSRRAYSQLSKCHTSLTSIYEAILEQESSPIPFTDATYYRVGFYGKSFGSLHCKEYVYREPRDVRLGDIMKNLGNIYEPTVIEGKQNLHIIPDSRQVNPEDLQAGICYLQITSVDPVTEDEDLESRRERQSGRSSSTFSARVFDRFLFDTPFTKNGRSQGGLEDQWKRRTVLWTEGPFPSLVNRLMVVKSESREFSPIENAIGMIETRTAALAGELDDQRINEGDPLPRLQGLQRILQGSVAVQVNSGVLGVCTAFLSGEPSTRLRSQELQQLIAALLEFMAVCKKAIRVHARLIGEEDQDFHSQLVIGFQSLTAELSHYIPAILSDVTILGKHSNVLKL

Target ORF information:

RefSeq Version	XM_024513552.1
Organism	Physcomitrella patens
Definition	Physcomitrella patens guanine nucleotide exchange factor SPIKE 1-like (LOC112279271), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024513552.1

ORF Insert Sequence:

ATGCATTTGG TCGCTGGAGC GCCTATATGG TCGGTGGAAA GCGTCAAGGA GGGAAAAGGA 
TCCAGGTTCG ACGTGTCGGA CTCAGTAGGA CAATGTAGGG TAGGTAGTCA TGATCGGAGG 
GACGAGGAGG AGACATGGGG ACTCAATGCA GAGACAGAAA GCTCTGCATT CTGGGAGCTG 
AGCTTCGAGA CTAGAGCTGT CGTCGACAGC GGATCAATTA AGAAGCAGGG GATGGATGAA 
ATTGCCTTGA GCGGGCAGCG GTTCAAACGT ATCCCACACC CGTACATCGG GCAGTCAACA 
GATCTTGAGT CCTTGCTGAA TGGGAATGGA GACCCTTTCC CACATTTCAA AGAGCTTCTT 
CAAAGCTACA GGGCTGAATG GGTGAAGGAT GAGTTCAAAT ACGGTCATTA TGAAGCTGTG 
CCACCGCCAG CCTTAGAACA TCAGATTTTT GAAGGACCCG ACACTGACAT ACAGACAGAG 
TTTCGGCTTT CTAACGTCAG ACTCGGCAGA AATGGTGATG TTACTGATGA TGAAGATCCT 
CAAACTCCAC GACGGCTTTC AAGACGTTTT TCTGTACGGT CATCTGGTCG TTTCTCTATG 
AGATCTACGA CTAGTGATGC ACCTGAAGCA TTGCACACAA ATATCTCAAA GCATTATGGG 
GTTGCTCCGT TACCTCCTTA TGAGCCCGTT TATAATTGGC GAGCAACAAG GGCATCTGTA 
CTTGGACAAA GAACTTCGGA ACTTCCATCA GCAATATCAT CTAGTAGCGG GCTGAAGGTT 
TCTGTGAAGG TTGTCTCTTT GAATGTTCAG GCCGGACTTG TCGAACCCAT GTACGGTACC 
TTGTGTCTCT ATCATCGAGA GAAGCGGGAG AAATTATCAG AAAATTTTCA CTTCCGATGT 
TTACCATCAG AGTTCCAAGA TTGTGTAACT TGGCAGGAGG GTGGTAATTG TCTGAGAAGA 
GCTATATTTT CACTGGAGGC AGCGTCGCCA GCAATATGTT TGTTGATCCA GCTTGAAAAG 
CATGTGACTG AGGAAGGAGG AGTAACGGCT CATGTTTATT CTCGAAAAGA ACCTGTACAC 
TTGACCGAGA GAGAAAAGCA GAAGTTACAG GTCTGGGCGC GCGTGATGCC ATTCAGGGAG 
CCTTTCGCGT GGGCAGTAGT TGCTCTGTTT GATGCAAATA TAACAGGAGG TGTAGGAGGA 
TTCTCATCTT CTCCAAGCAG CCCACTTCCA CCAGGACTTT TTGGACCATC TTTGATGGAA 
GCATCCTTGG ATTCTGATGG ACGACTTATA GCTGATGCTA AGACAGAATC ATCTAGTGTG 
CCAGTTTTGG TTGATGTTCA GAGCCTTCGC CGGGTCAAAG AAAATTACAC GGAGGAAATG 
CTTCAGGATC CCAAGTACAA AGTGCACAAA CCCGTGAAGG CCATCCTTCG TCTCGAGATA 
GAGAGGTTAT CTACTGAAGA TCATGACCAA GATTCCATAT TTGAGTGTGG TAGTTTTGGT 
CGTGGGTCCA CTGATGGTGA TTCCCGCTTG ACAGTAGGAC CCTCTTTCTC AGGGCGTTTC 
ATGCAGGGGC TGGGCATGAC GAAGGTTACG CATAATGGGC GGCAGAAATG GAGCCCTGGA 
AGAAAACATC GCTATCTGCA TTCCGGCAGT GTTGGCAGCA TTGAATTTGG AAAGTTTGAC 
TTCCGGGCTA TTGAGTTTCG ATCGGGTTCG AAAGTTGAGG TTTTACCTCA GCTATTGCAC 
TGCCTGTATG TATATCCTCA ATCAGTCAAT TTAAGTCGAA AACGCAATCT TTTTGTGCGA 
GTGGAGCTTC GAAAGGATGA TGTAGACATT CGCAAATCTC CTCTCGAGGC CCTTTATCCG 
AGGGATGCTG ACAGCTCTAT GCAGAAATAT GCACACTCTC AGATCGATGC AAACACCAAG 
ACACCTCACT ATCATGATGA GTTCAAAATT CAACTACCTG CCGTTCTCTC TCCTGAGCAT 
CACCTTCTGT TCACGTTTTT TCATGTGGAT CTACAGATGA AAATTGAGGC TCCAAAGCCG 
GTGGTAGTGG GATACTCTGT TCTTCCGCTC ATGTTTGTTT GTCAGGTTCA GCGGTTGGAC 
GGCAGCCTAC CTATCGCAAA GGAGCTGTAT TCCAATTATC TACAAGAAAA TGTGAAGGAA 
AGGTTGGAGT ACTTGGATGA TGGCAAAACT GTATTTAAAT TGAGGTCACG ATTATGCTCT 
TCTCTTTATC CGGTTAATGA GAGGATCCGA GATTTCTTTT CAGAATACGA TCGACATGTC 
TTGCGGACCA GTCCACCCTG GGGTACTGAG CTGATGGAGG CTATTAATGC TCTGAAGACG 
GTTGAGCCGT CAGCTATGCT CCAGTTTCTT CAACCTATTC TCAACATGCT GCTTCGTATG 
ATTCGCGATG GAGGAGAAAC GTTGCAGGTT GCTGCATTCC GTTCAATGGT TAACATCATT 
ACCAGGGTAC AAGCAGAGAC ATCTGATGGG GCTGAAAGAA ATCGTTACCT TGTGCATTAT 
GTGGATTATG CATTTAATGA TTTTGGTGGG CGTCATGAGC CTGTGTACCC AGGTCTGTGC 
AGTGTATGGC GCAGCCTTGC TCGAAGCAAG GCGAAAGGCT ACCGTGTCGG ACCTGTGTAT 
GATGATGTTC TTTCCATGGC TTGGGTGTTC CTGGAGCTAA TTGTCAAGTC CATGGCACTT 
GAACAATCCC GCATTTACTC TGAGTCCCTC CCACCTGGAG AGGAGTTACC ACCTTTGCAG 
CTCATTGATG AGGTCTTTAA AAGTATCGTA CAGCTCTATG ATTGTTTACT GACAGAGGTT 
CATGAGCGCT GCAAGAAGGG CATGCTTCTC GCAATGAAGC TGAATAGCAG TATTGCATAC 
TTTTGCTATG ACCTTCTCTC TGTAATTGAG CATCGACAAG TCTTTGAGCT GGTTGCGCTT 
TATTTCAACA AGTTTGCTGG GGTATGTCAA TCTCTTGTGC ACGAATGTAA GCTTAATTTT 
CTGCGCATCG TCTGTGACCA TGATCTCTTT GTTGAGATGC CCGGTCGAGA TCCTACAGAG 
AGGAATTATT TGGCATCACT GTTGATGCAA GAGCTCTTTG TTACTTGGGA TCATGAAGAT 
CTGACTTTAA GATCTAAGGC AGCTCGAATC CTTGTGCAAC TTATGTGCAA GCATGAGTAT 
GATGCACGGT ATCAAACATT GGAGGACAAA ATGTATATTG CCCAACGTTA CTTTCCACTA 
GTTGACCTGA TATTGGACGA AATGCCAGTG TTCTATGGCC TGAGCACTAC AGAGAAGCGT 
GAAGTGCTGG TGTGTGTGCT TCACATTCTG CGATATCTAG ACGATGCATC TCTTGTTAAA 
GCTTGGCAGC ACAACATGGA TAGAACAAAG CTTTTTTTCA AACTCCTGGA AGAATGTCAG 
GAATTGCTGG AGTATAAGAA GGCTGGAGCA GATGGCTTGA TGGGAGCTAT GCCAGCGAAT 
GATCAAGGAG AAGGGCCCCT TCGATACTCC GAGAAGCTCT CACCTGCAGT CAATCAGTTT 
TTGATTGAGG CATCTCGGCA GGATGTCAGA CTCACAAAGA TTTCTCCAAA TGCTCAGTGG 
ATACTTCCAC AAACGAGTCC AGAGATTGGT TCATTCTGGA AAAGAGCAAG CCCTGTAACA 
AATAGTTCAA CTAGCCCAGG TCAGCCGCAT TCGTTGCGAG AGGCACTAGC CCAAGCACAG 
TCTGCGGGAA GAAGTGGAGG CAGGTCTCTT CGTGAGACCT TGCATCCTAT GCTCCGCCAA 
AAGTTGGAGC TATGGGAGGA GAACCTTAGT GCCTCTGCTA TGCTCCAAAT TTTGGAGGTA 
CTTGAGAAGT TTATAAGTGC AGTATCAAGT CAAGCTGTTC TCACTGACTA CATGAGGCTT 
GATTGTATCA CAACTATTTT TATTGGTTTT CTGGGACGCA GCCAGCCGTT GCCATTTTGG 
AAGGCCTTTC TACCTTTCTT CAATAACGTG TTTCGTCGTC ATGGCGCTAC CTTGATGAGT 
AGGGAGAATG ATAGGTTCTT GAAGCAAGTA GCATTTCATC TACTACGGCT CGGAGTTTTC 
CGGAATGAGT CCATACGGAA ACGTTCTGTT GTTGGCCTTC AAATTCTTGT CCGAAATGCA 
TACCACTACT TTCAAGGCCT TTCAAGACTG CGGGTGATGC TTACAATCAC ACTTTCTGAG 
CTCATGTCAG ATGTTCAAGT AACACATCCT AAGATGGATG GATCCTTGGA AGAGAGTGGT 
GAATCTCAAC GTCTTCGTAG GTCCTTGCAA CAGATTGCAC AAGAAAGTAT TAGTGCGGAT 
CTTCTACGGG AATGTGGTCT TCCTGAAGAT TCTCTTACTG CTTTGCCAGA AGGTGTGAAT 
GGCCAACAAT GGAGTTGGGC TGGAGTCGCT GAGCTTTCCA CCTCTCTGCT GAAAGCTGTG 
GATGCTGCTG TAGGACATGC TCTTTTGGGT CCTGAGATTT TAATGAATGA CAAGTATGCA 
ACTGCAGAGG CCTACCATGG TTTGGCGACG GCATATACCA ACGTGCCCGA TCTGCATATA 
ATGTGGCTTC TGTATCTCTG CGAAGTTCAT CAAGGCAACC AGTCTTATGC AGAGGCTGCT 
CAGTGTGCTG TTGCGGTTGC AGGTGTCATC ATGCATGCTA TAGTAGGAAA AGGTGATCAT 
GTTTGGGGAA AAGAGCATGT TGAGGCACTG GGTAATATTT TCCCAATGCT CACTGGTTCC 
CGCTTTGGGG ATGCTGCAAC TGCCGAAATT GAAGGCTATG GGTCATCGAA ATTGACTGTC 
GAATCGGCAG TGAAGTACTT GCAGCTCGCC AATAAACTCT TTGTCCAGGC AGAGCTCTTC 
CATTTCTGTG CCGGCATATT GGAGCTCATA ATTCCCGTCC ATAAGTCAAG GCGTGCCTAT 
AGTCAGCTGT CTAAATGTCA TACTTCATTG ACCAGCATCT ACGAAGCTAT ACTAGAGCAA 
GAGTCGAGCC CTATCCCTTT CACGGATGCA ACATATTATC GTGTGGGATT TTATGGGAAA 
AGCTTTGGTA GTTTGCACTG CAAGGAGTAT GTGTACAGGG AACCACGGGA CGTGCGGTTA 
GGGGACATTA TGAAAAATTT AGGAAATATT TATGAGCCTA CAGTAATTGA AGGAAAGCAG 
AACTTGCATA TTATCCCCGA TTCTCGTCAA GTGAATCCAG AGGATCTTCA AGCTGGCATT 
TGTTACTTGC AAATTACATC TGTCGATCCT GTTACAGAGG ACGAGGATTT GGAAAGCAGG 
CGAGAAAGAC AATCCGGGAG GTCCTCGTCA ACATTCAGTG CACGAGTCTT CGATCGATTT 
TTATTTGACA CTCCTTTCAC AAAGAATGGA AGATCCCAAG GAGGTTTGGA AGATCAGTGG 
AAGAGGCGTA CGGTTTTGTG GACTGAAGGG CCTTTTCCAT CTCTTGTAAA TCGTTTAATG 
GTTGTGAAGT CTGAGTCGCG AGAATTTTCG CCAATCGAAA ACGCCATAGG AATGATTGAG 
ACTCGTACAG CAGCTTTAGC AGGAGAGCTC GATGATCAAC GTATAAATGA GGGAGATCCT 
CTGCCCCGTT TACAAGGCCT GCAAAGAATC TTGCAAGGAA GTGTTGCAGT CCAGGTAAAT 
AGTGGAGTGC TAGGTGTCTG CACAGCTTTC TTGTCTGGGG AACCTTCAAC ACGATTGCGC 
TCACAAGAAC TACAGCAGCT GATAGCAGCA CTCTTGGAGT TTATGGCTGT GTGCAAGAAA 
GCAATTCGAG TACATGCACG ACTGATTGGA GAGGAGGACC AAGACTTCCA CTCGCAGCTT 
GTGATTGGAT TCCAATCACT CACAGCAGAG CTATCCCACT ATATTCCCGC CATCTTGTCA 
GATGTAACCA TACTTGGGAA ACACAGCAAT GTGCTCAAGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC112279271 ( XM_024513585.1 ) cDNA ORF clone, -> XP_024369353.1 Physcomitrella patens guanine nucleotide exchange factor SPIKE 1-like (LOC112279271), transcript variant X7, mRNA.

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CloneID	OPn41280
Clone ID Related Accession (Same CDS sequence)	XM_024513560.1 , XM_024513543.1 , XM_024513534.1 , XM_024513569.1 , XM_024513576.1 , XM_024513552.1 , XM_024513585.1
Accession Version	XM_024513585.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Physcomitrella patens
Product	guanine nucleotide exchange factor SPIKE 1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037253.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physcomitrella patens Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATTTGG TCGCTGGAGC GCCTATATGG TCGGTGGAAA GCGTCAAGGA GGGAAAAGGA 
TCCAGGTTCG ACGTGTCGGA CTCAGTAGGA CAATGTAGGG TAGGTAGTCA TGATCGGAGG 
GACGAGGAGG AGACATGGGG ACTCAATGCA GAGACAGAAA GCTCTGCATT CTGGGAGCTG 
AGCTTCGAGA CTAGAGCTGT CGTCGACAGC GGATCAATTA AGAAGCAGGG GATGGATGAA 
ATTGCCTTGA GCGGGCAGCG GTTCAAACGT ATCCCACACC CGTACATCGG GCAGTCAACA 
GATCTTGAGT CCTTGCTGAA TGGGAATGGA GACCCTTTCC CACATTTCAA AGAGCTTCTT 
CAAAGCTACA GGGCTGAATG GGTGAAGGAT GAGTTCAAAT ACGGTCATTA TGAAGCTGTG 
CCACCGCCAG CCTTAGAACA TCAGATTTTT GAAGGACCCG ACACTGACAT ACAGACAGAG 
TTTCGGCTTT CTAACGTCAG ACTCGGCAGA AATGGTGATG TTACTGATGA TGAAGATCCT 
CAAACTCCAC GACGGCTTTC AAGACGTTTT TCTGTACGGT CATCTGGTCG TTTCTCTATG 
AGATCTACGA CTAGTGATGC ACCTGAAGCA TTGCACACAA ATATCTCAAA GCATTATGGG 
GTTGCTCCGT TACCTCCTTA TGAGCCCGTT TATAATTGGC GAGCAACAAG GGCATCTGTA 
CTTGGACAAA GAACTTCGGA ACTTCCATCA GCAATATCAT CTAGTAGCGG GCTGAAGGTT 
TCTGTGAAGG TTGTCTCTTT GAATGTTCAG GCCGGACTTG TCGAACCCAT GTACGGTACC 
TTGTGTCTCT ATCATCGAGA GAAGCGGGAG AAATTATCAG AAAATTTTCA CTTCCGATGT 
TTACCATCAG AGTTCCAAGA TTGTGTAACT TGGCAGGAGG GTGGTAATTG TCTGAGAAGA 
GCTATATTTT CACTGGAGGC AGCGTCGCCA GCAATATGTT TGTTGATCCA GCTTGAAAAG 
CATGTGACTG AGGAAGGAGG AGTAACGGCT CATGTTTATT CTCGAAAAGA ACCTGTACAC 
TTGACCGAGA GAGAAAAGCA GAAGTTACAG GTCTGGGCGC GCGTGATGCC ATTCAGGGAG 
CCTTTCGCGT GGGCAGTAGT TGCTCTGTTT GATGCAAATA TAACAGGAGG TGTAGGAGGA 
TTCTCATCTT CTCCAAGCAG CCCACTTCCA CCAGGACTTT TTGGACCATC TTTGATGGAA 
GCATCCTTGG ATTCTGATGG ACGACTTATA GCTGATGCTA AGACAGAATC ATCTAGTGTG 
CCAGTTTTGG TTGATGTTCA GAGCCTTCGC CGGGTCAAAG AAAATTACAC GGAGGAAATG 
CTTCAGGATC CCAAGTACAA AGTGCACAAA CCCGTGAAGG CCATCCTTCG TCTCGAGATA 
GAGAGGTTAT CTACTGAAGA TCATGACCAA GATTCCATAT TTGAGTGTGG TAGTTTTGGT 
CGTGGGTCCA CTGATGGTGA TTCCCGCTTG ACAGTAGGAC CCTCTTTCTC AGGGCGTTTC 
ATGCAGGGGC TGGGCATGAC GAAGGTTACG CATAATGGGC GGCAGAAATG GAGCCCTGGA 
AGAAAACATC GCTATCTGCA TTCCGGCAGT GTTGGCAGCA TTGAATTTGG AAAGTTTGAC 
TTCCGGGCTA TTGAGTTTCG ATCGGGTTCG AAAGTTGAGG TTTTACCTCA GCTATTGCAC 
TGCCTGTATG TATATCCTCA ATCAGTCAAT TTAAGTCGAA AACGCAATCT TTTTGTGCGA 
GTGGAGCTTC GAAAGGATGA TGTAGACATT CGCAAATCTC CTCTCGAGGC CCTTTATCCG 
AGGGATGCTG ACAGCTCTAT GCAGAAATAT GCACACTCTC AGATCGATGC AAACACCAAG 
ACACCTCACT ATCATGATGA GTTCAAAATT CAACTACCTG CCGTTCTCTC TCCTGAGCAT 
CACCTTCTGT TCACGTTTTT TCATGTGGAT CTACAGATGA AAATTGAGGC TCCAAAGCCG 
GTGGTAGTGG GATACTCTGT TCTTCCGCTC ATGTTTGTTT GTCAGGTTCA GCGGTTGGAC 
GGCAGCCTAC CTATCGCAAA GGAGCTGTAT TCCAATTATC TACAAGAAAA TGTGAAGGAA 
AGGTTGGAGT ACTTGGATGA TGGCAAAACT GTATTTAAAT TGAGGTCACG ATTATGCTCT 
TCTCTTTATC CGGTTAATGA GAGGATCCGA GATTTCTTTT CAGAATACGA TCGACATGTC 
TTGCGGACCA GTCCACCCTG GGGTACTGAG CTGATGGAGG CTATTAATGC TCTGAAGACG 
GTTGAGCCGT CAGCTATGCT CCAGTTTCTT CAACCTATTC TCAACATGCT GCTTCGTATG 
ATTCGCGATG GAGGAGAAAC GTTGCAGGTT GCTGCATTCC GTTCAATGGT TAACATCATT 
ACCAGGGTAC AAGCAGAGAC ATCTGATGGG GCTGAAAGAA ATCGTTACCT TGTGCATTAT 
GTGGATTATG CATTTAATGA TTTTGGTGGG CGTCATGAGC CTGTGTACCC AGGTCTGTGC 
AGTGTATGGC GCAGCCTTGC TCGAAGCAAG GCGAAAGGCT ACCGTGTCGG ACCTGTGTAT 
GATGATGTTC TTTCCATGGC TTGGGTGTTC CTGGAGCTAA TTGTCAAGTC CATGGCACTT 
GAACAATCCC GCATTTACTC TGAGTCCCTC CCACCTGGAG AGGAGTTACC ACCTTTGCAG 
CTCATTGATG AGGTCTTTAA AAGTATCGTA CAGCTCTATG ATTGTTTACT GACAGAGGTT 
CATGAGCGCT GCAAGAAGGG CATGCTTCTC GCAATGAAGC TGAATAGCAG TATTGCATAC 
TTTTGCTATG ACCTTCTCTC TGTAATTGAG CATCGACAAG TCTTTGAGCT GGTTGCGCTT 
TATTTCAACA AGTTTGCTGG GGTATGTCAA TCTCTTGTGC ACGAATGTAA GCTTAATTTT 
CTGCGCATCG TCTGTGACCA TGATCTCTTT GTTGAGATGC CCGGTCGAGA TCCTACAGAG 
AGGAATTATT TGGCATCACT GTTGATGCAA GAGCTCTTTG TTACTTGGGA TCATGAAGAT 
CTGACTTTAA GATCTAAGGC AGCTCGAATC CTTGTGCAAC TTATGTGCAA GCATGAGTAT 
GATGCACGGT ATCAAACATT GGAGGACAAA ATGTATATTG CCCAACGTTA CTTTCCACTA 
GTTGACCTGA TATTGGACGA AATGCCAGTG TTCTATGGCC TGAGCACTAC AGAGAAGCGT 
GAAGTGCTGG TGTGTGTGCT TCACATTCTG CGATATCTAG ACGATGCATC TCTTGTTAAA 
GCTTGGCAGC ACAACATGGA TAGAACAAAG CTTTTTTTCA AACTCCTGGA AGAATGTCAG 
GAATTGCTGG AGTATAAGAA GGCTGGAGCA GATGGCTTGA TGGGAGCTAT GCCAGCGAAT 
GATCAAGGAG AAGGGCCCCT TCGATACTCC GAGAAGCTCT CACCTGCAGT CAATCAGTTT 
TTGATTGAGG CATCTCGGCA GGATGTCAGA CTCACAAAGA TTTCTCCAAA TGCTCAGTGG 
ATACTTCCAC AAACGAGTCC AGAGATTGGT TCATTCTGGA AAAGAGCAAG CCCTGTAACA 
AATAGTTCAA CTAGCCCAGG TCAGCCGCAT TCGTTGCGAG AGGCACTAGC CCAAGCACAG 
TCTGCGGGAA GAAGTGGAGG CAGGTCTCTT CGTGAGACCT TGCATCCTAT GCTCCGCCAA 
AAGTTGGAGC TATGGGAGGA GAACCTTAGT GCCTCTGCTA TGCTCCAAAT TTTGGAGGTA 
CTTGAGAAGT TTATAAGTGC AGTATCAAGT CAAGCTGTTC TCACTGACTA CATGAGGCTT 
GATTGTATCA CAACTATTTT TATTGGTTTT CTGGGACGCA GCCAGCCGTT GCCATTTTGG 
AAGGCCTTTC TACCTTTCTT CAATAACGTG TTTCGTCGTC ATGGCGCTAC CTTGATGAGT 
AGGGAGAATG ATAGGTTCTT GAAGCAAGTA GCATTTCATC TACTACGGCT CGGAGTTTTC 
CGGAATGAGT CCATACGGAA ACGTTCTGTT GTTGGCCTTC AAATTCTTGT CCGAAATGCA 
TACCACTACT TTCAAGGCCT TTCAAGACTG CGGGTGATGC TTACAATCAC ACTTTCTGAG 
CTCATGTCAG ATGTTCAAGT AACACATCCT AAGATGGATG GATCCTTGGA AGAGAGTGGT 
GAATCTCAAC GTCTTCGTAG GTCCTTGCAA CAGATTGCAC AAGAAAGTAT TAGTGCGGAT 
CTTCTACGGG AATGTGGTCT TCCTGAAGAT TCTCTTACTG CTTTGCCAGA AGGTGTGAAT 
GGCCAACAAT GGAGTTGGGC TGGAGTCGCT GAGCTTTCCA CCTCTCTGCT GAAAGCTGTG 
GATGCTGCTG TAGGACATGC TCTTTTGGGT CCTGAGATTT TAATGAATGA CAAGTATGCA 
ACTGCAGAGG CCTACCATGG TTTGGCGACG GCATATACCA ACGTGCCCGA TCTGCATATA 
ATGTGGCTTC TGTATCTCTG CGAAGTTCAT CAAGGCAACC AGTCTTATGC AGAGGCTGCT 
CAGTGTGCTG TTGCGGTTGC AGGTGTCATC ATGCATGCTA TAGTAGGAAA AGGTGATCAT 
GTTTGGGGAA AAGAGCATGT TGAGGCACTG GGTAATATTT TCCCAATGCT CACTGGTTCC 
CGCTTTGGGG ATGCTGCAAC TGCCGAAATT GAAGGCTATG GGTCATCGAA ATTGACTGTC 
GAATCGGCAG TGAAGTACTT GCAGCTCGCC AATAAACTCT TTGTCCAGGC AGAGCTCTTC 
CATTTCTGTG CCGGCATATT GGAGCTCATA ATTCCCGTCC ATAAGTCAAG GCGTGCCTAT 
AGTCAGCTGT CTAAATGTCA TACTTCATTG ACCAGCATCT ACGAAGCTAT ACTAGAGCAA 
GAGTCGAGCC CTATCCCTTT CACGGATGCA ACATATTATC GTGTGGGATT TTATGGGAAA 
AGCTTTGGTA GTTTGCACTG CAAGGAGTAT GTGTACAGGG AACCACGGGA CGTGCGGTTA 
GGGGACATTA TGAAAAATTT AGGAAATATT TATGAGCCTA CAGTAATTGA AGGAAAGCAG 
AACTTGCATA TTATCCCCGA TTCTCGTCAA GTGAATCCAG AGGATCTTCA AGCTGGCATT 
TGTTACTTGC AAATTACATC TGTCGATCCT GTTACAGAGG ACGAGGATTT GGAAAGCAGG 
CGAGAAAGAC AATCCGGGAG GTCCTCGTCA ACATTCAGTG CACGAGTCTT CGATCGATTT 
TTATTTGACA CTCCTTTCAC AAAGAATGGA AGATCCCAAG GAGGTTTGGA AGATCAGTGG 
AAGAGGCGTA CGGTTTTGTG GACTGAAGGG CCTTTTCCAT CTCTTGTAAA TCGTTTAATG 
GTTGTGAAGT CTGAGTCGCG AGAATTTTCG CCAATCGAAA ACGCCATAGG AATGATTGAG 
ACTCGTACAG CAGCTTTAGC AGGAGAGCTC GATGATCAAC GTATAAATGA GGGAGATCCT 
CTGCCCCGTT TACAAGGCCT GCAAAGAATC TTGCAAGGAA GTGTTGCAGT CCAGGTAAAT 
AGTGGAGTGC TAGGTGTCTG CACAGCTTTC TTGTCTGGGG AACCTTCAAC ACGATTGCGC 
TCACAAGAAC TACAGCAGCT GATAGCAGCA CTCTTGGAGT TTATGGCTGT GTGCAAGAAA 
GCAATTCGAG TACATGCACG ACTGATTGGA GAGGAGGACC AAGACTTCCA CTCGCAGCTT 
GTGATTGGAT TCCAATCACT CACAGCAGAG CTATCCCACT ATATTCCCGC CATCTTGTCA 
GATGTAACCA TACTTGGGAA ACACAGCAAT GTGCTCAAGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024369353.1
CDS	348..6272
Translation	MHLVAGAPIWSVESVKEGKGSRFDVSDSVGQCRVGSHDRRDEEETWGLNAETESSAFWELSFETRAVVDSGSIKKQGMDEIALSGQRFKRIPHPYIGQSTDLESLLNGNGDPFPHFKELLQSYRAEWVKDEFKYGHYEAVPPPALEHQIFEGPDTDIQTEFRLSNVRLGRNGDVTDDEDPQTPRRLSRRFSVRSSGRFSMRSTTSDAPEALHTNISKHYGVAPLPPYEPVYNWRATRASVLGQRTSELPSAISSSSGLKVSVKVVSLNVQAGLVEPMYGTLCLYHREKREKLSENFHFRCLPSEFQDCVTWQEGGNCLRRAIFSLEAASPAICLLIQLEKHVTEEGGVTAHVYSRKEPVHLTEREKQKLQVWARVMPFREPFAWAVVALFDANITGGVGGFSSSPSSPLPPGLFGPSLMEASLDSDGRLIADAKTESSSVPVLVDVQSLRRVKENYTEEMLQDPKYKVHKPVKAILRLEIERLSTEDHDQDSIFECGSFGRGSTDGDSRLTVGPSFSGRFMQGLGMTKVTHNGRQKWSPGRKHRYLHSGSVGSIEFGKFDFRAIEFRSGSKVEVLPQLLHCLYVYPQSVNLSRKRNLFVRVELRKDDVDIRKSPLEALYPRDADSSMQKYAHSQIDANTKTPHYHDEFKIQLPAVLSPEHHLLFTFFHVDLQMKIEAPKPVVVGYSVLPLMFVCQVQRLDGSLPIAKELYSNYLQENVKERLEYLDDGKTVFKLRSRLCSSLYPVNERIRDFFSEYDRHVLRTSPPWGTELMEAINALKTVEPSAMLQFLQPILNMLLRMIRDGGETLQVAAFRSMVNIITRVQAETSDGAERNRYLVHYVDYAFNDFGGRHEPVYPGLCSVWRSLARSKAKGYRVGPVYDDVLSMAWVFLELIVKSMALEQSRIYSESLPPGEELPPLQLIDEVFKSIVQLYDCLLTEVHERCKKGMLLAMKLNSSIAYFCYDLLSVIEHRQVFELVALYFNKFAGVCQSLVHECKLNFLRIVCDHDLFVEMPGRDPTERNYLASLLMQELFVTWDHEDLTLRSKAARILVQLMCKHEYDARYQTLEDKMYIAQRYFPLVDLILDEMPVFYGLSTTEKREVLVCVLHILRYLDDASLVKAWQHNMDRTKLFFKLLEECQELLEYKKAGADGLMGAMPANDQGEGPLRYSEKLSPAVNQFLIEASRQDVRLTKISPNAQWILPQTSPEIGSFWKRASPVTNSSTSPGQPHSLREALAQAQSAGRSGGRSLRETLHPMLRQKLELWEENLSASAMLQILEVLEKFISAVSSQAVLTDYMRLDCITTIFIGFLGRSQPLPFWKAFLPFFNNVFRRHGATLMSRENDRFLKQVAFHLLRLGVFRNESIRKRSVVGLQILVRNAYHYFQGLSRLRVMLTITLSELMSDVQVTHPKMDGSLEESGESQRLRRSLQQIAQESISADLLRECGLPEDSLTALPEGVNGQQWSWAGVAELSTSLLKAVDAAVGHALLGPEILMNDKYATAEAYHGLATAYTNVPDLHIMWLLYLCEVHQGNQSYAEAAQCAVAVAGVIMHAIVGKGDHVWGKEHVEALGNIFPMLTGSRFGDAATAEIEGYGSSKLTVESAVKYLQLANKLFVQAELFHFCAGILELIIPVHKSRRAYSQLSKCHTSLTSIYEAILEQESSPIPFTDATYYRVGFYGKSFGSLHCKEYVYREPRDVRLGDIMKNLGNIYEPTVIEGKQNLHIIPDSRQVNPEDLQAGICYLQITSVDPVTEDEDLESRRERQSGRSSSTFSARVFDRFLFDTPFTKNGRSQGGLEDQWKRRTVLWTEGPFPSLVNRLMVVKSESREFSPIENAIGMIETRTAALAGELDDQRINEGDPLPRLQGLQRILQGSVAVQVNSGVLGVCTAFLSGEPSTRLRSQELQQLIAALLEFMAVCKKAIRVHARLIGEEDQDFHSQLVIGFQSLTAELSHYIPAILSDVTILGKHSNVLKL

Target ORF information:

RefSeq Version	XM_024513585.1
Organism	Physcomitrella patens
Definition	Physcomitrella patens guanine nucleotide exchange factor SPIKE 1-like (LOC112279271), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024513585.1

ORF Insert Sequence:

ATGCATTTGG TCGCTGGAGC GCCTATATGG TCGGTGGAAA GCGTCAAGGA GGGAAAAGGA 
TCCAGGTTCG ACGTGTCGGA CTCAGTAGGA CAATGTAGGG TAGGTAGTCA TGATCGGAGG 
GACGAGGAGG AGACATGGGG ACTCAATGCA GAGACAGAAA GCTCTGCATT CTGGGAGCTG 
AGCTTCGAGA CTAGAGCTGT CGTCGACAGC GGATCAATTA AGAAGCAGGG GATGGATGAA 
ATTGCCTTGA GCGGGCAGCG GTTCAAACGT ATCCCACACC CGTACATCGG GCAGTCAACA 
GATCTTGAGT CCTTGCTGAA TGGGAATGGA GACCCTTTCC CACATTTCAA AGAGCTTCTT 
CAAAGCTACA GGGCTGAATG GGTGAAGGAT GAGTTCAAAT ACGGTCATTA TGAAGCTGTG 
CCACCGCCAG CCTTAGAACA TCAGATTTTT GAAGGACCCG ACACTGACAT ACAGACAGAG 
TTTCGGCTTT CTAACGTCAG ACTCGGCAGA AATGGTGATG TTACTGATGA TGAAGATCCT 
CAAACTCCAC GACGGCTTTC AAGACGTTTT TCTGTACGGT CATCTGGTCG TTTCTCTATG 
AGATCTACGA CTAGTGATGC ACCTGAAGCA TTGCACACAA ATATCTCAAA GCATTATGGG 
GTTGCTCCGT TACCTCCTTA TGAGCCCGTT TATAATTGGC GAGCAACAAG GGCATCTGTA 
CTTGGACAAA GAACTTCGGA ACTTCCATCA GCAATATCAT CTAGTAGCGG GCTGAAGGTT 
TCTGTGAAGG TTGTCTCTTT GAATGTTCAG GCCGGACTTG TCGAACCCAT GTACGGTACC 
TTGTGTCTCT ATCATCGAGA GAAGCGGGAG AAATTATCAG AAAATTTTCA CTTCCGATGT 
TTACCATCAG AGTTCCAAGA TTGTGTAACT TGGCAGGAGG GTGGTAATTG TCTGAGAAGA 
GCTATATTTT CACTGGAGGC AGCGTCGCCA GCAATATGTT TGTTGATCCA GCTTGAAAAG 
CATGTGACTG AGGAAGGAGG AGTAACGGCT CATGTTTATT CTCGAAAAGA ACCTGTACAC 
TTGACCGAGA GAGAAAAGCA GAAGTTACAG GTCTGGGCGC GCGTGATGCC ATTCAGGGAG 
CCTTTCGCGT GGGCAGTAGT TGCTCTGTTT GATGCAAATA TAACAGGAGG TGTAGGAGGA 
TTCTCATCTT CTCCAAGCAG CCCACTTCCA CCAGGACTTT TTGGACCATC TTTGATGGAA 
GCATCCTTGG ATTCTGATGG ACGACTTATA GCTGATGCTA AGACAGAATC ATCTAGTGTG 
CCAGTTTTGG TTGATGTTCA GAGCCTTCGC CGGGTCAAAG AAAATTACAC GGAGGAAATG 
CTTCAGGATC CCAAGTACAA AGTGCACAAA CCCGTGAAGG CCATCCTTCG TCTCGAGATA 
GAGAGGTTAT CTACTGAAGA TCATGACCAA GATTCCATAT TTGAGTGTGG TAGTTTTGGT 
CGTGGGTCCA CTGATGGTGA TTCCCGCTTG ACAGTAGGAC CCTCTTTCTC AGGGCGTTTC 
ATGCAGGGGC TGGGCATGAC GAAGGTTACG CATAATGGGC GGCAGAAATG GAGCCCTGGA 
AGAAAACATC GCTATCTGCA TTCCGGCAGT GTTGGCAGCA TTGAATTTGG AAAGTTTGAC 
TTCCGGGCTA TTGAGTTTCG ATCGGGTTCG AAAGTTGAGG TTTTACCTCA GCTATTGCAC 
TGCCTGTATG TATATCCTCA ATCAGTCAAT TTAAGTCGAA AACGCAATCT TTTTGTGCGA 
GTGGAGCTTC GAAAGGATGA TGTAGACATT CGCAAATCTC CTCTCGAGGC CCTTTATCCG 
AGGGATGCTG ACAGCTCTAT GCAGAAATAT GCACACTCTC AGATCGATGC AAACACCAAG 
ACACCTCACT ATCATGATGA GTTCAAAATT CAACTACCTG CCGTTCTCTC TCCTGAGCAT 
CACCTTCTGT TCACGTTTTT TCATGTGGAT CTACAGATGA AAATTGAGGC TCCAAAGCCG 
GTGGTAGTGG GATACTCTGT TCTTCCGCTC ATGTTTGTTT GTCAGGTTCA GCGGTTGGAC 
GGCAGCCTAC CTATCGCAAA GGAGCTGTAT TCCAATTATC TACAAGAAAA TGTGAAGGAA 
AGGTTGGAGT ACTTGGATGA TGGCAAAACT GTATTTAAAT TGAGGTCACG ATTATGCTCT 
TCTCTTTATC CGGTTAATGA GAGGATCCGA GATTTCTTTT CAGAATACGA TCGACATGTC 
TTGCGGACCA GTCCACCCTG GGGTACTGAG CTGATGGAGG CTATTAATGC TCTGAAGACG 
GTTGAGCCGT CAGCTATGCT CCAGTTTCTT CAACCTATTC TCAACATGCT GCTTCGTATG 
ATTCGCGATG GAGGAGAAAC GTTGCAGGTT GCTGCATTCC GTTCAATGGT TAACATCATT 
ACCAGGGTAC AAGCAGAGAC ATCTGATGGG GCTGAAAGAA ATCGTTACCT TGTGCATTAT 
GTGGATTATG CATTTAATGA TTTTGGTGGG CGTCATGAGC CTGTGTACCC AGGTCTGTGC 
AGTGTATGGC GCAGCCTTGC TCGAAGCAAG GCGAAAGGCT ACCGTGTCGG ACCTGTGTAT 
GATGATGTTC TTTCCATGGC TTGGGTGTTC CTGGAGCTAA TTGTCAAGTC CATGGCACTT 
GAACAATCCC GCATTTACTC TGAGTCCCTC CCACCTGGAG AGGAGTTACC ACCTTTGCAG 
CTCATTGATG AGGTCTTTAA AAGTATCGTA CAGCTCTATG ATTGTTTACT GACAGAGGTT 
CATGAGCGCT GCAAGAAGGG CATGCTTCTC GCAATGAAGC TGAATAGCAG TATTGCATAC 
TTTTGCTATG ACCTTCTCTC TGTAATTGAG CATCGACAAG TCTTTGAGCT GGTTGCGCTT 
TATTTCAACA AGTTTGCTGG GGTATGTCAA TCTCTTGTGC ACGAATGTAA GCTTAATTTT 
CTGCGCATCG TCTGTGACCA TGATCTCTTT GTTGAGATGC CCGGTCGAGA TCCTACAGAG 
AGGAATTATT TGGCATCACT GTTGATGCAA GAGCTCTTTG TTACTTGGGA TCATGAAGAT 
CTGACTTTAA GATCTAAGGC AGCTCGAATC CTTGTGCAAC TTATGTGCAA GCATGAGTAT 
GATGCACGGT ATCAAACATT GGAGGACAAA ATGTATATTG CCCAACGTTA CTTTCCACTA 
GTTGACCTGA TATTGGACGA AATGCCAGTG TTCTATGGCC TGAGCACTAC AGAGAAGCGT 
GAAGTGCTGG TGTGTGTGCT TCACATTCTG CGATATCTAG ACGATGCATC TCTTGTTAAA 
GCTTGGCAGC ACAACATGGA TAGAACAAAG CTTTTTTTCA AACTCCTGGA AGAATGTCAG 
GAATTGCTGG AGTATAAGAA GGCTGGAGCA GATGGCTTGA TGGGAGCTAT GCCAGCGAAT 
GATCAAGGAG AAGGGCCCCT TCGATACTCC GAGAAGCTCT CACCTGCAGT CAATCAGTTT 
TTGATTGAGG CATCTCGGCA GGATGTCAGA CTCACAAAGA TTTCTCCAAA TGCTCAGTGG 
ATACTTCCAC AAACGAGTCC AGAGATTGGT TCATTCTGGA AAAGAGCAAG CCCTGTAACA 
AATAGTTCAA CTAGCCCAGG TCAGCCGCAT TCGTTGCGAG AGGCACTAGC CCAAGCACAG 
TCTGCGGGAA GAAGTGGAGG CAGGTCTCTT CGTGAGACCT TGCATCCTAT GCTCCGCCAA 
AAGTTGGAGC TATGGGAGGA GAACCTTAGT GCCTCTGCTA TGCTCCAAAT TTTGGAGGTA 
CTTGAGAAGT TTATAAGTGC AGTATCAAGT CAAGCTGTTC TCACTGACTA CATGAGGCTT 
GATTGTATCA CAACTATTTT TATTGGTTTT CTGGGACGCA GCCAGCCGTT GCCATTTTGG 
AAGGCCTTTC TACCTTTCTT CAATAACGTG TTTCGTCGTC ATGGCGCTAC CTTGATGAGT 
AGGGAGAATG ATAGGTTCTT GAAGCAAGTA GCATTTCATC TACTACGGCT CGGAGTTTTC 
CGGAATGAGT CCATACGGAA ACGTTCTGTT GTTGGCCTTC AAATTCTTGT CCGAAATGCA 
TACCACTACT TTCAAGGCCT TTCAAGACTG CGGGTGATGC TTACAATCAC ACTTTCTGAG 
CTCATGTCAG ATGTTCAAGT AACACATCCT AAGATGGATG GATCCTTGGA AGAGAGTGGT 
GAATCTCAAC GTCTTCGTAG GTCCTTGCAA CAGATTGCAC AAGAAAGTAT TAGTGCGGAT 
CTTCTACGGG AATGTGGTCT TCCTGAAGAT TCTCTTACTG CTTTGCCAGA AGGTGTGAAT 
GGCCAACAAT GGAGTTGGGC TGGAGTCGCT GAGCTTTCCA CCTCTCTGCT GAAAGCTGTG 
GATGCTGCTG TAGGACATGC TCTTTTGGGT CCTGAGATTT TAATGAATGA CAAGTATGCA 
ACTGCAGAGG CCTACCATGG TTTGGCGACG GCATATACCA ACGTGCCCGA TCTGCATATA 
ATGTGGCTTC TGTATCTCTG CGAAGTTCAT CAAGGCAACC AGTCTTATGC AGAGGCTGCT 
CAGTGTGCTG TTGCGGTTGC AGGTGTCATC ATGCATGCTA TAGTAGGAAA AGGTGATCAT 
GTTTGGGGAA AAGAGCATGT TGAGGCACTG GGTAATATTT TCCCAATGCT CACTGGTTCC 
CGCTTTGGGG ATGCTGCAAC TGCCGAAATT GAAGGCTATG GGTCATCGAA ATTGACTGTC 
GAATCGGCAG TGAAGTACTT GCAGCTCGCC AATAAACTCT TTGTCCAGGC AGAGCTCTTC 
CATTTCTGTG CCGGCATATT GGAGCTCATA ATTCCCGTCC ATAAGTCAAG GCGTGCCTAT 
AGTCAGCTGT CTAAATGTCA TACTTCATTG ACCAGCATCT ACGAAGCTAT ACTAGAGCAA 
GAGTCGAGCC CTATCCCTTT CACGGATGCA ACATATTATC GTGTGGGATT TTATGGGAAA 
AGCTTTGGTA GTTTGCACTG CAAGGAGTAT GTGTACAGGG AACCACGGGA CGTGCGGTTA 
GGGGACATTA TGAAAAATTT AGGAAATATT TATGAGCCTA CAGTAATTGA AGGAAAGCAG 
AACTTGCATA TTATCCCCGA TTCTCGTCAA GTGAATCCAG AGGATCTTCA AGCTGGCATT 
TGTTACTTGC AAATTACATC TGTCGATCCT GTTACAGAGG ACGAGGATTT GGAAAGCAGG 
CGAGAAAGAC AATCCGGGAG GTCCTCGTCA ACATTCAGTG CACGAGTCTT CGATCGATTT 
TTATTTGACA CTCCTTTCAC AAAGAATGGA AGATCCCAAG GAGGTTTGGA AGATCAGTGG 
AAGAGGCGTA CGGTTTTGTG GACTGAAGGG CCTTTTCCAT CTCTTGTAAA TCGTTTAATG 
GTTGTGAAGT CTGAGTCGCG AGAATTTTCG CCAATCGAAA ACGCCATAGG AATGATTGAG 
ACTCGTACAG CAGCTTTAGC AGGAGAGCTC GATGATCAAC GTATAAATGA GGGAGATCCT 
CTGCCCCGTT TACAAGGCCT GCAAAGAATC TTGCAAGGAA GTGTTGCAGT CCAGGTAAAT 
AGTGGAGTGC TAGGTGTCTG CACAGCTTTC TTGTCTGGGG AACCTTCAAC ACGATTGCGC 
TCACAAGAAC TACAGCAGCT GATAGCAGCA CTCTTGGAGT TTATGGCTGT GTGCAAGAAA 
GCAATTCGAG TACATGCACG ACTGATTGGA GAGGAGGACC AAGACTTCCA CTCGCAGCTT 
GTGATTGGAT TCCAATCACT CACAGCAGAG CTATCCCACT ATATTCCCGC CATCTTGTCA 
GATGTAACCA TACTTGGGAA ACACAGCAAT GTGCTCAAGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC112279271 ( XM_024513595.1 ) cDNA ORF clone, -> XP_024369363.1 Physcomitrella patens guanine nucleotide exchange factor SPIKE 1-like (LOC112279271), transcript variant X8, mRNA.

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CloneID	OPn41281
Clone ID Related Accession (Same CDS sequence)	XM_024513595.1
Accession Version	XM_024513595.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Physcomitrella patens
Product	guanine nucleotide exchange factor SPIKE 1-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037253.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physcomitrella patens Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATTTGG TCGCTGGAGC GCCTATATGG TCGGTGGAAA GCGTCAAGGA GGGAAAAGGA 
TCCAGGTTCG ACGTGTCGGA CTCAGTAGGA CAATGTAGGG TAGGTAGTCA TGATCGGAGG 
GACGAGGAGG AGACATGGGG ACTCAATGCA GAGACAGAAA GCTCTGCATT CTGGGAGCTG 
AGCTTCGAGA CTAGAGCTGT CGTCGACAGC GGATCAATTA AGAAGGGGAT GGATGAAATT 
GCCTTGAGCG GGCAGCGGTT CAAACGTATC CCACACCCGT ACATCGGGCA GTCAACAGAT 
CTTGAGTCCT TGCTGAATGG GAATGGAGAC CCTTTCCCAC ATTTCAAAGA GCTTCTTCAA 
AGCTACAGGG CTGAATGGGT GAAGGATGAG TTCAAATACG GTCATTATGA AGCTGTGCCA 
CCGCCAGCCT TAGAACATCA GATTTTTGAA GGACCCGACA CTGACATACA GACAGAGTTT 
CGGCTTTCTA ACGTCAGACT CGGCAGAAAT GGTGATGTTA CTGATGATGA AGATCCTCAA 
ACTCCACGAC GGCTTTCAAG ACGTTTTTCT GTACGGTCAT CTGGTCGTTT CTCTATGAGA 
TCTACGACTA GTGATGCACC TGAAGCATTG CACACAAATA TCTCAAAGCA TTATGGGGTT 
GCTCCGTTAC CTCCTTATGA GCCCGTTTAT AATTGGCGAG CAACAAGGGC ATCTGTACTT 
GGACAAAGAA CTTCGGAACT TCCATCAGCA ATATCATCTA GTAGCGGGCT GAAGGTTTCT 
GTGAAGGTTG TCTCTTTGAA TGTTCAGGCC GGACTTGTCG AACCCATGTA CGGTACCTTG 
TGTCTCTATC ATCGAGAGAA GCGGGAGAAA TTATCAGAAA ATTTTCACTT CCGATGTTTA 
CCATCAGAGT TCCAAGATTG TGTAACTTGG CAGGAGGGTG GTAATTGTCT GAGAAGAGCT 
ATATTTTCAC TGGAGGCAGC GTCGCCAGCA ATATGTTTGT TGATCCAGCT TGAAAAGCAT 
GTGACTGAGG AAGGAGGAGT AACGGCTCAT GTTTATTCTC GAAAAGAACC TGTACACTTG 
ACCGAGAGAG AAAAGCAGAA GTTACAGGTC TGGGCGCGCG TGATGCCATT CAGGGAGCCT 
TTCGCGTGGG CAGTAGTTGC TCTGTTTGAT GCAAATATAA CAGGAGGTGT AGGAGGATTC 
TCATCTTCTC CAAGCAGCCC ACTTCCACCA GGACTTTTTG GACCATCTTT GATGGAAGCA 
TCCTTGGATT CTGATGGACG ACTTATAGCT GATGCTAAGA CAGAATCATC TAGTGTGCCA 
GTTTTGGTTG ATGTTCAGAG CCTTCGCCGG GTCAAAGAAA ATTACACGGA GGAAATGCTT 
CAGGATCCCA AGTACAAAGT GCACAAACCC GTGAAGGCCA TCCTTCGTCT CGAGATAGAG 
AGGTTATCTA CTGAAGATCA TGACCAAGAT TCCATATTTG AGTGTGGTAG TTTTGGTCGT 
GGGTCCACTG ATGGTGATTC CCGCTTGACA GTAGGACCCT CTTTCTCAGG GCGTTTCATG 
CAGGGGCTGG GCATGACGAA GGTTACGCAT AATGGGCGGC AGAAATGGAG CCCTGGAAGA 
AAACATCGCT ATCTGCATTC CGGCAGTGTT GGCAGCATTG AATTTGGAAA GTTTGACTTC 
CGGGCTATTG AGTTTCGATC GGGTTCGAAA GTTGAGGTTT TACCTCAGCT ATTGCACTGC 
CTGTATGTAT ATCCTCAATC AGTCAATTTA AGTCGAAAAC GCAATCTTTT TGTGCGAGTG 
GAGCTTCGAA AGGATGATGT AGACATTCGC AAATCTCCTC TCGAGGCCCT TTATCCGAGG 
GATGCTGACA GCTCTATGCA GAAATATGCA CACTCTCAGA TCGATGCAAA CACCAAGACA 
CCTCACTATC ATGATGAGTT CAAAATTCAA CTACCTGCCG TTCTCTCTCC TGAGCATCAC 
CTTCTGTTCA CGTTTTTTCA TGTGGATCTA CAGATGAAAA TTGAGGCTCC AAAGCCGGTG 
GTAGTGGGAT ACTCTGTTCT TCCGCTCATG TTTGTTTGTC AGGTTCAGCG GTTGGACGGC 
AGCCTACCTA TCGCAAAGGA GCTGTATTCC AATTATCTAC AAGAAAATGT GAAGGAAAGG 
TTGGAGTACT TGGATGATGG CAAAACTGTA TTTAAATTGA GGTCACGATT ATGCTCTTCT 
CTTTATCCGG TTAATGAGAG GATCCGAGAT TTCTTTTCAG AATACGATCG ACATGTCTTG 
CGGACCAGTC CACCCTGGGG TACTGAGCTG ATGGAGGCTA TTAATGCTCT GAAGACGGTT 
GAGCCGTCAG CTATGCTCCA GTTTCTTCAA CCTATTCTCA ACATGCTGCT TCGTATGATT 
CGCGATGGAG GAGAAACGTT GCAGGTTGCT GCATTCCGTT CAATGGTTAA CATCATTACC 
AGGGTACAAG CAGAGACATC TGATGGGGCT GAAAGAAATC GTTACCTTGT GCATTATGTG 
GATTATGCAT TTAATGATTT TGGTGGGCGT CATGAGCCTG TGTACCCAGG TCTGTGCAGT 
GTATGGCGCA GCCTTGCTCG AAGCAAGGCG AAAGGCTACC GTGTCGGACC TGTGTATGAT 
GATGTTCTTT CCATGGCTTG GGTGTTCCTG GAGCTAATTG TCAAGTCCAT GGCACTTGAA 
CAATCCCGCA TTTACTCTGA GTCCCTCCCA CCTGGAGAGG AGTTACCACC TTTGCAGCTC 
ATTGATGAGG TCTTTAAAAG TATCGTACAG CTCTATGATT GTTTACTGAC AGAGGTTCAT 
GAGCGCTGCA AGAAGGGCAT GCTTCTCGCA ATGAAGCTGA ATAGCAGTAT TGCATACTTT 
TGCTATGACC TTCTCTCTGT AATTGAGCAT CGACAAGTCT TTGAGCTGGT TGCGCTTTAT 
TTCAACAAGT TTGCTGGGGT ATGTCAATCT CTTGTGCACG AATGTAAGCT TAATTTTCTG 
CGCATCGTCT GTGACCATGA TCTCTTTGTT GAGATGCCCG GTCGAGATCC TACAGAGAGG 
AATTATTTGG CATCACTGTT GATGCAAGAG CTCTTTGTTA CTTGGGATCA TGAAGATCTG 
ACTTTAAGAT CTAAGGCAGC TCGAATCCTT GTGCAACTTA TGTGCAAGCA TGAGTATGAT 
GCACGGTATC AAACATTGGA GGACAAAATG TATATTGCCC AACGTTACTT TCCACTAGTT 
GACCTGATAT TGGACGAAAT GCCAGTGTTC TATGGCCTGA GCACTACAGA GAAGCGTGAA 
GTGCTGGTGT GTGTGCTTCA CATTCTGCGA TATCTAGACG ATGCATCTCT TGTTAAAGCT 
TGGCAGCACA ACATGGATAG AACAAAGCTT TTTTTCAAAC TCCTGGAAGA ATGTCAGGAA 
TTGCTGGAGT ATAAGAAGGC TGGAGCAGAT GGCTTGATGG GAGCTATGCC AGCGAATGAT 
CAAGGAGAAG GGCCCCTTCG ATACTCCGAG AAGCTCTCAC CTGCAGTCAA TCAGTTTTTG 
ATTGAGGCAT CTCGGCAGGA TGTCAGACTC ACAAAGATTT CTCCAAATGC TCAGTGGATA 
CTTCCACAAA CGAGTCCAGA GATTGGTTCA TTCTGGAAAA GAGCAAGCCC TGTAACAAAT 
AGTTCAACTA GCCCAGGTCA GCCGCATTCG TTGCGAGAGG CACTAGCCCA AGCACAGTCT 
GCGGGAAGAA GTGGAGGCAG GTCTCTTCGT GAGACCTTGC ATCCTATGCT CCGCCAAAAG 
TTGGAGCTAT GGGAGGAGAA CCTTAGTGCC TCTGCTATGC TCCAAATTTT GGAGGTACTT 
GAGAAGTTTA TAAGTGCAGT ATCAAGTCAA GCTGTTCTCA CTGACTACAT GAGGCTTGAT 
TGTATCACAA CTATTTTTAT TGGTTTTCTG GGACGCAGCC AGCCGTTGCC ATTTTGGAAG 
GCCTTTCTAC CTTTCTTCAA TAACGTGTTT CGTCGTCATG GCGCTACCTT GATGAGTAGG 
GAGAATGATA GGTTCTTGAA GCAAGTAGCA TTTCATCTAC TACGGCTCGG AGTTTTCCGG 
AATGAGTCCA TACGGAAACG TTCTGTTGTT GGCCTTCAAA TTCTTGTCCG AAATGCATAC 
CACTACTTTC AAGGCCTTTC AAGACTGCGG GTGATGCTTA CAATCACACT TTCTGAGCTC 
ATGTCAGATG TTCAAGTAAC ACATCCTAAG ATGGATGGAT CCTTGGAAGA GAGTGGTGAA 
TCTCAACGTC TTCGTAGGTC CTTGCAACAG ATTGCACAAG AAAGTATTAG TGCGGATCTT 
CTACGGGAAT GTGGTCTTCC TGAAGATTCT CTTACTGCTT TGCCAGAAGG TGTGAATGGC 
CAACAATGGA GTTGGGCTGG AGTCGCTGAG CTTTCCACCT CTCTGCTGAA AGCTGTGGAT 
GCTGCTGTAG GACATGCTCT TTTGGGTCCT GAGATTTTAA TGAATGACAA GTATGCAACT 
GCAGAGGCCT ACCATGGTTT GGCGACGGCA TATACCAACG TGCCCGATCT GCATATAATG 
TGGCTTCTGT ATCTCTGCGA AGTTCATCAA GGCAACCAGT CTTATGCAGA GGCTGCTCAG 
TGTGCTGTTG CGGTTGCAGG TGTCATCATG CATGCTATAG TAGGAAAAGG TGATCATGTT 
TGGGGAAAAG AGCATGTTGA GGCACTGGGT AATATTTTCC CAATGCTCAC TGGTTCCCGC 
TTTGGGGATG CTGCAACTGC CGAAATTGAA GGCTATGGGT CATCGAAATT GACTGTCGAA 
TCGGCAGTGA AGTACTTGCA GCTCGCCAAT AAACTCTTTG TCCAGGCAGA GCTCTTCCAT 
TTCTGTGCCG GCATATTGGA GCTCATAATT CCCGTCCATA AGTCAAGGCG TGCCTATAGT 
CAGCTGTCTA AATGTCATAC TTCATTGACC AGCATCTACG AAGCTATACT AGAGCAAGAG 
TCGAGCCCTA TCCCTTTCAC GGATGCAACA TATTATCGTG TGGGATTTTA TGGGAAAAGC 
TTTGGTAGTT TGCACTGCAA GGAGTATGTG TACAGGGAAC CACGGGACGT GCGGTTAGGG 
GACATTATGA AAAATTTAGG AAATATTTAT GAGCCTACAG TAATTGAAGG AAAGCAGAAC 
TTGCATATTA TCCCCGATTC TCGTCAAGTG AATCCAGAGG ATCTTCAAGC TGGCATTTGT 
TACTTGCAAA TTACATCTGT CGATCCTGTT ACAGAGGACG AGGATTTGGA AAGCAGGCGA 
GAAAGACAAT CCGGGAGGTC CTCGTCAACA TTCAGTGCAC GAGTCTTCGA TCGATTTTTA 
TTTGACACTC CTTTCACAAA GAATGGAAGA TCCCAAGGAG GTTTGGAAGA TCAGTGGAAG 
AGGCGTACGG TTTTGTGGAC TGAAGGGCCT TTTCCATCTC TTGTAAATCG TTTAATGGTT 
GTGAAGTCTG AGTCGCGAGA ATTTTCGCCA ATCGAAAACG CCATAGGAAT GATTGAGACT 
CGTACAGCAG CTTTAGCAGG AGAGCTCGAT GATCAACGTA TAAATGAGGG AGATCCTCTG 
CCCCGTTTAC AAGGCCTGCA AAGAATCTTG CAAGGAAGTG TTGCAGTCCA GGTAAATAGT 
GGAGTGCTAG GTGTCTGCAC AGCTTTCTTG TCTGGGGAAC CTTCAACACG ATTGCGCTCA 
CAAGAACTAC AGCAGCTGAT AGCAGCACTC TTGGAGTTTA TGGCTGTGTG CAAGAAAGCA 
ATTCGAGTAC ATGCACGACT GATTGGAGAG GAGGACCAAG ACTTCCACTC GCAGCTTGTG 
ATTGGATTCC AATCACTCAC AGCAGAGCTA TCCCACTATA TTCCCGCCAT CTTGTCAGAT 
GTAACCATAC TTGGGAAACA CAGCAATGTG CTCAAGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024369363.1
CDS	348..6269
Translation	MHLVAGAPIWSVESVKEGKGSRFDVSDSVGQCRVGSHDRRDEEETWGLNAETESSAFWELSFETRAVVDSGSIKKGMDEIALSGQRFKRIPHPYIGQSTDLESLLNGNGDPFPHFKELLQSYRAEWVKDEFKYGHYEAVPPPALEHQIFEGPDTDIQTEFRLSNVRLGRNGDVTDDEDPQTPRRLSRRFSVRSSGRFSMRSTTSDAPEALHTNISKHYGVAPLPPYEPVYNWRATRASVLGQRTSELPSAISSSSGLKVSVKVVSLNVQAGLVEPMYGTLCLYHREKREKLSENFHFRCLPSEFQDCVTWQEGGNCLRRAIFSLEAASPAICLLIQLEKHVTEEGGVTAHVYSRKEPVHLTEREKQKLQVWARVMPFREPFAWAVVALFDANITGGVGGFSSSPSSPLPPGLFGPSLMEASLDSDGRLIADAKTESSSVPVLVDVQSLRRVKENYTEEMLQDPKYKVHKPVKAILRLEIERLSTEDHDQDSIFECGSFGRGSTDGDSRLTVGPSFSGRFMQGLGMTKVTHNGRQKWSPGRKHRYLHSGSVGSIEFGKFDFRAIEFRSGSKVEVLPQLLHCLYVYPQSVNLSRKRNLFVRVELRKDDVDIRKSPLEALYPRDADSSMQKYAHSQIDANTKTPHYHDEFKIQLPAVLSPEHHLLFTFFHVDLQMKIEAPKPVVVGYSVLPLMFVCQVQRLDGSLPIAKELYSNYLQENVKERLEYLDDGKTVFKLRSRLCSSLYPVNERIRDFFSEYDRHVLRTSPPWGTELMEAINALKTVEPSAMLQFLQPILNMLLRMIRDGGETLQVAAFRSMVNIITRVQAETSDGAERNRYLVHYVDYAFNDFGGRHEPVYPGLCSVWRSLARSKAKGYRVGPVYDDVLSMAWVFLELIVKSMALEQSRIYSESLPPGEELPPLQLIDEVFKSIVQLYDCLLTEVHERCKKGMLLAMKLNSSIAYFCYDLLSVIEHRQVFELVALYFNKFAGVCQSLVHECKLNFLRIVCDHDLFVEMPGRDPTERNYLASLLMQELFVTWDHEDLTLRSKAARILVQLMCKHEYDARYQTLEDKMYIAQRYFPLVDLILDEMPVFYGLSTTEKREVLVCVLHILRYLDDASLVKAWQHNMDRTKLFFKLLEECQELLEYKKAGADGLMGAMPANDQGEGPLRYSEKLSPAVNQFLIEASRQDVRLTKISPNAQWILPQTSPEIGSFWKRASPVTNSSTSPGQPHSLREALAQAQSAGRSGGRSLRETLHPMLRQKLELWEENLSASAMLQILEVLEKFISAVSSQAVLTDYMRLDCITTIFIGFLGRSQPLPFWKAFLPFFNNVFRRHGATLMSRENDRFLKQVAFHLLRLGVFRNESIRKRSVVGLQILVRNAYHYFQGLSRLRVMLTITLSELMSDVQVTHPKMDGSLEESGESQRLRRSLQQIAQESISADLLRECGLPEDSLTALPEGVNGQQWSWAGVAELSTSLLKAVDAAVGHALLGPEILMNDKYATAEAYHGLATAYTNVPDLHIMWLLYLCEVHQGNQSYAEAAQCAVAVAGVIMHAIVGKGDHVWGKEHVEALGNIFPMLTGSRFGDAATAEIEGYGSSKLTVESAVKYLQLANKLFVQAELFHFCAGILELIIPVHKSRRAYSQLSKCHTSLTSIYEAILEQESSPIPFTDATYYRVGFYGKSFGSLHCKEYVYREPRDVRLGDIMKNLGNIYEPTVIEGKQNLHIIPDSRQVNPEDLQAGICYLQITSVDPVTEDEDLESRRERQSGRSSSTFSARVFDRFLFDTPFTKNGRSQGGLEDQWKRRTVLWTEGPFPSLVNRLMVVKSESREFSPIENAIGMIETRTAALAGELDDQRINEGDPLPRLQGLQRILQGSVAVQVNSGVLGVCTAFLSGEPSTRLRSQELQQLIAALLEFMAVCKKAIRVHARLIGEEDQDFHSQLVIGFQSLTAELSHYIPAILSDVTILGKHSNVLKL

Target ORF information:

RefSeq Version	XM_024513595.1
Organism	Physcomitrella patens
Definition	Physcomitrella patens guanine nucleotide exchange factor SPIKE 1-like (LOC112279271), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024513595.1

ORF Insert Sequence:

ATGCATTTGG TCGCTGGAGC GCCTATATGG TCGGTGGAAA GCGTCAAGGA GGGAAAAGGA 
TCCAGGTTCG ACGTGTCGGA CTCAGTAGGA CAATGTAGGG TAGGTAGTCA TGATCGGAGG 
GACGAGGAGG AGACATGGGG ACTCAATGCA GAGACAGAAA GCTCTGCATT CTGGGAGCTG 
AGCTTCGAGA CTAGAGCTGT CGTCGACAGC GGATCAATTA AGAAGGGGAT GGATGAAATT 
GCCTTGAGCG GGCAGCGGTT CAAACGTATC CCACACCCGT ACATCGGGCA GTCAACAGAT 
CTTGAGTCCT TGCTGAATGG GAATGGAGAC CCTTTCCCAC ATTTCAAAGA GCTTCTTCAA 
AGCTACAGGG CTGAATGGGT GAAGGATGAG TTCAAATACG GTCATTATGA AGCTGTGCCA 
CCGCCAGCCT TAGAACATCA GATTTTTGAA GGACCCGACA CTGACATACA GACAGAGTTT 
CGGCTTTCTA ACGTCAGACT CGGCAGAAAT GGTGATGTTA CTGATGATGA AGATCCTCAA 
ACTCCACGAC GGCTTTCAAG ACGTTTTTCT GTACGGTCAT CTGGTCGTTT CTCTATGAGA 
TCTACGACTA GTGATGCACC TGAAGCATTG CACACAAATA TCTCAAAGCA TTATGGGGTT 
GCTCCGTTAC CTCCTTATGA GCCCGTTTAT AATTGGCGAG CAACAAGGGC ATCTGTACTT 
GGACAAAGAA CTTCGGAACT TCCATCAGCA ATATCATCTA GTAGCGGGCT GAAGGTTTCT 
GTGAAGGTTG TCTCTTTGAA TGTTCAGGCC GGACTTGTCG AACCCATGTA CGGTACCTTG 
TGTCTCTATC ATCGAGAGAA GCGGGAGAAA TTATCAGAAA ATTTTCACTT CCGATGTTTA 
CCATCAGAGT TCCAAGATTG TGTAACTTGG CAGGAGGGTG GTAATTGTCT GAGAAGAGCT 
ATATTTTCAC TGGAGGCAGC GTCGCCAGCA ATATGTTTGT TGATCCAGCT TGAAAAGCAT 
GTGACTGAGG AAGGAGGAGT AACGGCTCAT GTTTATTCTC GAAAAGAACC TGTACACTTG 
ACCGAGAGAG AAAAGCAGAA GTTACAGGTC TGGGCGCGCG TGATGCCATT CAGGGAGCCT 
TTCGCGTGGG CAGTAGTTGC TCTGTTTGAT GCAAATATAA CAGGAGGTGT AGGAGGATTC 
TCATCTTCTC CAAGCAGCCC ACTTCCACCA GGACTTTTTG GACCATCTTT GATGGAAGCA 
TCCTTGGATT CTGATGGACG ACTTATAGCT GATGCTAAGA CAGAATCATC TAGTGTGCCA 
GTTTTGGTTG ATGTTCAGAG CCTTCGCCGG GTCAAAGAAA ATTACACGGA GGAAATGCTT 
CAGGATCCCA AGTACAAAGT GCACAAACCC GTGAAGGCCA TCCTTCGTCT CGAGATAGAG 
AGGTTATCTA CTGAAGATCA TGACCAAGAT TCCATATTTG AGTGTGGTAG TTTTGGTCGT 
GGGTCCACTG ATGGTGATTC CCGCTTGACA GTAGGACCCT CTTTCTCAGG GCGTTTCATG 
CAGGGGCTGG GCATGACGAA GGTTACGCAT AATGGGCGGC AGAAATGGAG CCCTGGAAGA 
AAACATCGCT ATCTGCATTC CGGCAGTGTT GGCAGCATTG AATTTGGAAA GTTTGACTTC 
CGGGCTATTG AGTTTCGATC GGGTTCGAAA GTTGAGGTTT TACCTCAGCT ATTGCACTGC 
CTGTATGTAT ATCCTCAATC AGTCAATTTA AGTCGAAAAC GCAATCTTTT TGTGCGAGTG 
GAGCTTCGAA AGGATGATGT AGACATTCGC AAATCTCCTC TCGAGGCCCT TTATCCGAGG 
GATGCTGACA GCTCTATGCA GAAATATGCA CACTCTCAGA TCGATGCAAA CACCAAGACA 
CCTCACTATC ATGATGAGTT CAAAATTCAA CTACCTGCCG TTCTCTCTCC TGAGCATCAC 
CTTCTGTTCA CGTTTTTTCA TGTGGATCTA CAGATGAAAA TTGAGGCTCC AAAGCCGGTG 
GTAGTGGGAT ACTCTGTTCT TCCGCTCATG TTTGTTTGTC AGGTTCAGCG GTTGGACGGC 
AGCCTACCTA TCGCAAAGGA GCTGTATTCC AATTATCTAC AAGAAAATGT GAAGGAAAGG 
TTGGAGTACT TGGATGATGG CAAAACTGTA TTTAAATTGA GGTCACGATT ATGCTCTTCT 
CTTTATCCGG TTAATGAGAG GATCCGAGAT TTCTTTTCAG AATACGATCG ACATGTCTTG 
CGGACCAGTC CACCCTGGGG TACTGAGCTG ATGGAGGCTA TTAATGCTCT GAAGACGGTT 
GAGCCGTCAG CTATGCTCCA GTTTCTTCAA CCTATTCTCA ACATGCTGCT TCGTATGATT 
CGCGATGGAG GAGAAACGTT GCAGGTTGCT GCATTCCGTT CAATGGTTAA CATCATTACC 
AGGGTACAAG CAGAGACATC TGATGGGGCT GAAAGAAATC GTTACCTTGT GCATTATGTG 
GATTATGCAT TTAATGATTT TGGTGGGCGT CATGAGCCTG TGTACCCAGG TCTGTGCAGT 
GTATGGCGCA GCCTTGCTCG AAGCAAGGCG AAAGGCTACC GTGTCGGACC TGTGTATGAT 
GATGTTCTTT CCATGGCTTG GGTGTTCCTG GAGCTAATTG TCAAGTCCAT GGCACTTGAA 
CAATCCCGCA TTTACTCTGA GTCCCTCCCA CCTGGAGAGG AGTTACCACC TTTGCAGCTC 
ATTGATGAGG TCTTTAAAAG TATCGTACAG CTCTATGATT GTTTACTGAC AGAGGTTCAT 
GAGCGCTGCA AGAAGGGCAT GCTTCTCGCA ATGAAGCTGA ATAGCAGTAT TGCATACTTT 
TGCTATGACC TTCTCTCTGT AATTGAGCAT CGACAAGTCT TTGAGCTGGT TGCGCTTTAT 
TTCAACAAGT TTGCTGGGGT ATGTCAATCT CTTGTGCACG AATGTAAGCT TAATTTTCTG 
CGCATCGTCT GTGACCATGA TCTCTTTGTT GAGATGCCCG GTCGAGATCC TACAGAGAGG 
AATTATTTGG CATCACTGTT GATGCAAGAG CTCTTTGTTA CTTGGGATCA TGAAGATCTG 
ACTTTAAGAT CTAAGGCAGC TCGAATCCTT GTGCAACTTA TGTGCAAGCA TGAGTATGAT 
GCACGGTATC AAACATTGGA GGACAAAATG TATATTGCCC AACGTTACTT TCCACTAGTT 
GACCTGATAT TGGACGAAAT GCCAGTGTTC TATGGCCTGA GCACTACAGA GAAGCGTGAA 
GTGCTGGTGT GTGTGCTTCA CATTCTGCGA TATCTAGACG ATGCATCTCT TGTTAAAGCT 
TGGCAGCACA ACATGGATAG AACAAAGCTT TTTTTCAAAC TCCTGGAAGA ATGTCAGGAA 
TTGCTGGAGT ATAAGAAGGC TGGAGCAGAT GGCTTGATGG GAGCTATGCC AGCGAATGAT 
CAAGGAGAAG GGCCCCTTCG ATACTCCGAG AAGCTCTCAC CTGCAGTCAA TCAGTTTTTG 
ATTGAGGCAT CTCGGCAGGA TGTCAGACTC ACAAAGATTT CTCCAAATGC TCAGTGGATA 
CTTCCACAAA CGAGTCCAGA GATTGGTTCA TTCTGGAAAA GAGCAAGCCC TGTAACAAAT 
AGTTCAACTA GCCCAGGTCA GCCGCATTCG TTGCGAGAGG CACTAGCCCA AGCACAGTCT 
GCGGGAAGAA GTGGAGGCAG GTCTCTTCGT GAGACCTTGC ATCCTATGCT CCGCCAAAAG 
TTGGAGCTAT GGGAGGAGAA CCTTAGTGCC TCTGCTATGC TCCAAATTTT GGAGGTACTT 
GAGAAGTTTA TAAGTGCAGT ATCAAGTCAA GCTGTTCTCA CTGACTACAT GAGGCTTGAT 
TGTATCACAA CTATTTTTAT TGGTTTTCTG GGACGCAGCC AGCCGTTGCC ATTTTGGAAG 
GCCTTTCTAC CTTTCTTCAA TAACGTGTTT CGTCGTCATG GCGCTACCTT GATGAGTAGG 
GAGAATGATA GGTTCTTGAA GCAAGTAGCA TTTCATCTAC TACGGCTCGG AGTTTTCCGG 
AATGAGTCCA TACGGAAACG TTCTGTTGTT GGCCTTCAAA TTCTTGTCCG AAATGCATAC 
CACTACTTTC AAGGCCTTTC AAGACTGCGG GTGATGCTTA CAATCACACT TTCTGAGCTC 
ATGTCAGATG TTCAAGTAAC ACATCCTAAG ATGGATGGAT CCTTGGAAGA GAGTGGTGAA 
TCTCAACGTC TTCGTAGGTC CTTGCAACAG ATTGCACAAG AAAGTATTAG TGCGGATCTT 
CTACGGGAAT GTGGTCTTCC TGAAGATTCT CTTACTGCTT TGCCAGAAGG TGTGAATGGC 
CAACAATGGA GTTGGGCTGG AGTCGCTGAG CTTTCCACCT CTCTGCTGAA AGCTGTGGAT 
GCTGCTGTAG GACATGCTCT TTTGGGTCCT GAGATTTTAA TGAATGACAA GTATGCAACT 
GCAGAGGCCT ACCATGGTTT GGCGACGGCA TATACCAACG TGCCCGATCT GCATATAATG 
TGGCTTCTGT ATCTCTGCGA AGTTCATCAA GGCAACCAGT CTTATGCAGA GGCTGCTCAG 
TGTGCTGTTG CGGTTGCAGG TGTCATCATG CATGCTATAG TAGGAAAAGG TGATCATGTT 
TGGGGAAAAG AGCATGTTGA GGCACTGGGT AATATTTTCC CAATGCTCAC TGGTTCCCGC 
TTTGGGGATG CTGCAACTGC CGAAATTGAA GGCTATGGGT CATCGAAATT GACTGTCGAA 
TCGGCAGTGA AGTACTTGCA GCTCGCCAAT AAACTCTTTG TCCAGGCAGA GCTCTTCCAT 
TTCTGTGCCG GCATATTGGA GCTCATAATT CCCGTCCATA AGTCAAGGCG TGCCTATAGT 
CAGCTGTCTA AATGTCATAC TTCATTGACC AGCATCTACG AAGCTATACT AGAGCAAGAG 
TCGAGCCCTA TCCCTTTCAC GGATGCAACA TATTATCGTG TGGGATTTTA TGGGAAAAGC 
TTTGGTAGTT TGCACTGCAA GGAGTATGTG TACAGGGAAC CACGGGACGT GCGGTTAGGG 
GACATTATGA AAAATTTAGG AAATATTTAT GAGCCTACAG TAATTGAAGG AAAGCAGAAC 
TTGCATATTA TCCCCGATTC TCGTCAAGTG AATCCAGAGG ATCTTCAAGC TGGCATTTGT 
TACTTGCAAA TTACATCTGT CGATCCTGTT ACAGAGGACG AGGATTTGGA AAGCAGGCGA 
GAAAGACAAT CCGGGAGGTC CTCGTCAACA TTCAGTGCAC GAGTCTTCGA TCGATTTTTA 
TTTGACACTC CTTTCACAAA GAATGGAAGA TCCCAAGGAG GTTTGGAAGA TCAGTGGAAG 
AGGCGTACGG TTTTGTGGAC TGAAGGGCCT TTTCCATCTC TTGTAAATCG TTTAATGGTT 
GTGAAGTCTG AGTCGCGAGA ATTTTCGCCA ATCGAAAACG CCATAGGAAT GATTGAGACT 
CGTACAGCAG CTTTAGCAGG AGAGCTCGAT GATCAACGTA TAAATGAGGG AGATCCTCTG 
CCCCGTTTAC AAGGCCTGCA AAGAATCTTG CAAGGAAGTG TTGCAGTCCA GGTAAATAGT 
GGAGTGCTAG GTGTCTGCAC AGCTTTCTTG TCTGGGGAAC CTTCAACACG ATTGCGCTCA 
CAAGAACTAC AGCAGCTGAT AGCAGCACTC TTGGAGTTTA TGGCTGTGTG CAAGAAAGCA 
ATTCGAGTAC ATGCACGACT GATTGGAGAG GAGGACCAAG ACTTCCACTC GCAGCTTGTG 
ATTGGATTCC AATCACTCAC AGCAGAGCTA TCCCACTATA TTCCCGCCAT CTTGTCAGAT 
GTAACCATAC TTGGGAAACA CAGCAATGTG CTCAAGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC112279271 ( XM_024513604.1 ) cDNA ORF clone, -> XP_024369372.1 Physcomitrella patens guanine nucleotide exchange factor SPIKE 1-like (LOC112279271), transcript variant X9, mRNA.

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CloneID	OPn41282
Clone ID Related Accession (Same CDS sequence)	XM_024513604.1
Accession Version	XM_024513604.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Physcomitrella patens
Product	guanine nucleotide exchange factor SPIKE 1-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037253.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physcomitrella patens Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATTTGG TCGCTGGAGC GCCTATATGG TCGGTGGAAA GCGTCAAGGA GGGAAAAGGA 
TCCAGGTTCG ACGTGTCGGA CTCAGTAGGA CAATGTAGGG TAGGTAGTCA TGATCGGAGG 
GACGAGGAGG AGACATGGGG ACTCAATGCA GAGACAGAAA GCTCTGCATT CTGGGAGCTG 
AGCTTCGAGA CTAGAGCTGT CGTCGACAGC GGATCAATTA AGAAGCAGGG GATGGATGAA 
ATTGCCTTGA GCGGGCAGCG GTTCAAACGT ATCCCACACC CGTACATCGG GCAGTCAACA 
GATCTTGAGT CCTTGCTGAA TGGGAATGGA GACCCTTTCC CACATTTCAA AGAGCTTCTT 
CAAAGCTACA GGGCTGAATG GGTGAAGGAT GAGTTCAAAT ACGGTCATTA TGAAGCTGTG 
CCACCGCCAG CCTTAGAACA TCAGATTTTT GAAGGACCCG ACACTGACAT ACAGACAGAG 
TTTCGGCTTT CTAACGTCAG ACTCGGCAGA AATGGTGATG TTACTGATGA TGAAGATCCT 
CAAACTCCAC GACGGCTTTC AAGACGTTTT TCTGTACGGT CATCTGGTCG TTTCTCTATG 
AGATCTACGA CTAGTGATGC ACCTGAAGCA TTGCACACAA ATATCTCAAA GCATTATGGG 
GTTGCTCCGT TACCTCCTTA TGAGCCCGTT TATAATTGGC GAGCAACAAG GGCATCTGTA 
CTTGGACAAA GAACTTCGGA ACTTCCATCA GCAATATCAT CTAGCGGGCT GAAGGTTTCT 
GTGAAGGTTG TCTCTTTGAA TGTTCAGGCC GGACTTGTCG AACCCATGTA CGGTACCTTG 
TGTCTCTATC ATCGAGAGAA GCGGGAGAAA TTATCAGAAA ATTTTCACTT CCGATGTTTA 
CCATCAGAGT TCCAAGATTG TGTAACTTGG CAGGAGGGTG GTAATTGTCT GAGAAGAGCT 
ATATTTTCAC TGGAGGCAGC GTCGCCAGCA ATATGTTTGT TGATCCAGCT TGAAAAGCAT 
GTGACTGAGG AAGGAGGAGT AACGGCTCAT GTTTATTCTC GAAAAGAACC TGTACACTTG 
ACCGAGAGAG AAAAGCAGAA GTTACAGGTC TGGGCGCGCG TGATGCCATT CAGGGAGCCT 
TTCGCGTGGG CAGTAGTTGC TCTGTTTGAT GCAAATATAA CAGGAGGTGT AGGAGGATTC 
TCATCTTCTC CAAGCAGCCC ACTTCCACCA GGACTTTTTG GACCATCTTT GATGGAAGCA 
TCCTTGGATT CTGATGGACG ACTTATAGCT GATGCTAAGA CAGAATCATC TAGTGTGCCA 
GTTTTGGTTG ATGTTCAGAG CCTTCGCCGG GTCAAAGAAA ATTACACGGA GGAAATGCTT 
CAGGATCCCA AGTACAAAGT GCACAAACCC GTGAAGGCCA TCCTTCGTCT CGAGATAGAG 
AGGTTATCTA CTGAAGATCA TGACCAAGAT TCCATATTTG AGTGTGGTAG TTTTGGTCGT 
GGGTCCACTG ATGGTGATTC CCGCTTGACA GTAGGACCCT CTTTCTCAGG GCGTTTCATG 
CAGGGGCTGG GCATGACGAA GGTTACGCAT AATGGGCGGC AGAAATGGAG CCCTGGAAGA 
AAACATCGCT ATCTGCATTC CGGCAGTGTT GGCAGCATTG AATTTGGAAA GTTTGACTTC 
CGGGCTATTG AGTTTCGATC GGGTTCGAAA GTTGAGGTTT TACCTCAGCT ATTGCACTGC 
CTGTATGTAT ATCCTCAATC AGTCAATTTA AGTCGAAAAC GCAATCTTTT TGTGCGAGTG 
GAGCTTCGAA AGGATGATGT AGACATTCGC AAATCTCCTC TCGAGGCCCT TTATCCGAGG 
GATGCTGACA GCTCTATGCA GAAATATGCA CACTCTCAGA TCGATGCAAA CACCAAGACA 
CCTCACTATC ATGATGAGTT CAAAATTCAA CTACCTGCCG TTCTCTCTCC TGAGCATCAC 
CTTCTGTTCA CGTTTTTTCA TGTGGATCTA CAGATGAAAA TTGAGGCTCC AAAGCCGGTG 
GTAGTGGGAT ACTCTGTTCT TCCGCTCATG TTTGTTTGTC AGGTTCAGCG GTTGGACGGC 
AGCCTACCTA TCGCAAAGGA GCTGTATTCC AATTATCTAC AAGAAAATGT GAAGGAAAGG 
TTGGAGTACT TGGATGATGG CAAAACTGTA TTTAAATTGA GGTCACGATT ATGCTCTTCT 
CTTTATCCGG TTAATGAGAG GATCCGAGAT TTCTTTTCAG AATACGATCG ACATGTCTTG 
CGGACCAGTC CACCCTGGGG TACTGAGCTG ATGGAGGCTA TTAATGCTCT GAAGACGGTT 
GAGCCGTCAG CTATGCTCCA GTTTCTTCAA CCTATTCTCA ACATGCTGCT TCGTATGATT 
CGCGATGGAG GAGAAACGTT GCAGGTTGCT GCATTCCGTT CAATGGTTAA CATCATTACC 
AGGGTACAAG CAGAGACATC TGATGGGGCT GAAAGAAATC GTTACCTTGT GCATTATGTG 
GATTATGCAT TTAATGATTT TGGTGGGCGT CATGAGCCTG TGTACCCAGG TCTGTGCAGT 
GTATGGCGCA GCCTTGCTCG AAGCAAGGCG AAAGGCTACC GTGTCGGACC TGTGTATGAT 
GATGTTCTTT CCATGGCTTG GGTGTTCCTG GAGCTAATTG TCAAGTCCAT GGCACTTGAA 
CAATCCCGCA TTTACTCTGA GTCCCTCCCA CCTGGAGAGG AGTTACCACC TTTGCAGCTC 
ATTGATGAGG TCTTTAAAAG TATCGTACAG CTCTATGATT GTTTACTGAC AGAGGTTCAT 
GAGCGCTGCA AGAAGGGCAT GCTTCTCGCA ATGAAGCTGA ATAGCAGTAT TGCATACTTT 
TGCTATGACC TTCTCTCTGT AATTGAGCAT CGACAAGTCT TTGAGCTGGT TGCGCTTTAT 
TTCAACAAGT TTGCTGGGGT ATGTCAATCT CTTGTGCACG AATGTAAGCT TAATTTTCTG 
CGCATCGTCT GTGACCATGA TCTCTTTGTT GAGATGCCCG GTCGAGATCC TACAGAGAGG 
AATTATTTGG CATCACTGTT GATGCAAGAG CTCTTTGTTA CTTGGGATCA TGAAGATCTG 
ACTTTAAGAT CTAAGGCAGC TCGAATCCTT GTGCAACTTA TGTGCAAGCA TGAGTATGAT 
GCACGGTATC AAACATTGGA GGACAAAATG TATATTGCCC AACGTTACTT TCCACTAGTT 
GACCTGATAT TGGACGAAAT GCCAGTGTTC TATGGCCTGA GCACTACAGA GAAGCGTGAA 
GTGCTGGTGT GTGTGCTTCA CATTCTGCGA TATCTAGACG ATGCATCTCT TGTTAAAGCT 
TGGCAGCACA ACATGGATAG AACAAAGCTT TTTTTCAAAC TCCTGGAAGA ATGTCAGGAA 
TTGCTGGAGT ATAAGAAGGC TGGAGCAGAT GGCTTGATGG GAGCTATGCC AGCGAATGAT 
CAAGGAGAAG GGCCCCTTCG ATACTCCGAG AAGCTCTCAC CTGCAGTCAA TCAGTTTTTG 
ATTGAGGCAT CTCGGCAGGA TGTCAGACTC ACAAAGATTT CTCCAAATGC TCAGTGGATA 
CTTCCACAAA CGAGTCCAGA GATTGGTTCA TTCTGGAAAA GAGCAAGCCC TGTAACAAAT 
AGTTCAACTA GCCCAGGTCA GCCGCATTCG TTGCGAGAGG CACTAGCCCA AGCACAGTCT 
GCGGGAAGAA GTGGAGGCAG GTCTCTTCGT GAGACCTTGC ATCCTATGCT CCGCCAAAAG 
TTGGAGCTAT GGGAGGAGAA CCTTAGTGCC TCTGCTATGC TCCAAATTTT GGAGGTACTT 
GAGAAGTTTA TAAGTGCAGT ATCAAGTCAA GCTGTTCTCA CTGACTACAT GAGGCTTGAT 
TGTATCACAA CTATTTTTAT TGGTTTTCTG GGACGCAGCC AGCCGTTGCC ATTTTGGAAG 
GCCTTTCTAC CTTTCTTCAA TAACGTGTTT CGTCGTCATG GCGCTACCTT GATGAGTAGG 
GAGAATGATA GGTTCTTGAA GCAAGTAGCA TTTCATCTAC TACGGCTCGG AGTTTTCCGG 
AATGAGTCCA TACGGAAACG TTCTGTTGTT GGCCTTCAAA TTCTTGTCCG AAATGCATAC 
CACTACTTTC AAGGCCTTTC AAGACTGCGG GTGATGCTTA CAATCACACT TTCTGAGCTC 
ATGTCAGATG TTCAAGTAAC ACATCCTAAG ATGGATGGAT CCTTGGAAGA GAGTGGTGAA 
TCTCAACGTC TTCGTAGGTC CTTGCAACAG ATTGCACAAG AAAGTATTAG TGCGGATCTT 
CTACGGGAAT GTGGTCTTCC TGAAGATTCT CTTACTGCTT TGCCAGAAGG TGTGAATGGC 
CAACAATGGA GTTGGGCTGG AGTCGCTGAG CTTTCCACCT CTCTGCTGAA AGCTGTGGAT 
GCTGCTGTAG GACATGCTCT TTTGGGTCCT GAGATTTTAA TGAATGACAA GTATGCAACT 
GCAGAGGCCT ACCATGGTTT GGCGACGGCA TATACCAACG TGCCCGATCT GCATATAATG 
TGGCTTCTGT ATCTCTGCGA AGTTCATCAA GGCAACCAGT CTTATGCAGA GGCTGCTCAG 
TGTGCTGTTG CGGTTGCAGG TGTCATCATG CATGCTATAG TAGGAAAAGG TGATCATGTT 
TGGGGAAAAG AGCATGTTGA GGCACTGGGT AATATTTTCC CAATGCTCAC TGGTTCCCGC 
TTTGGGGATG CTGCAACTGC CGAAATTGAA GGCTATGGGT CATCGAAATT GACTGTCGAA 
TCGGCAGTGA AGTACTTGCA GCTCGCCAAT AAACTCTTTG TCCAGGCAGA GCTCTTCCAT 
TTCTGTGCCG GCATATTGGA GCTCATAATT CCCGTCCATA AGTCAAGGCG TGCCTATAGT 
CAGCTGTCTA AATGTCATAC TTCATTGACC AGCATCTACG AAGCTATACT AGAGCAAGAG 
TCGAGCCCTA TCCCTTTCAC GGATGCAACA TATTATCGTG TGGGATTTTA TGGGAAAAGC 
TTTGGTAGTT TGCACTGCAA GGAGTATGTG TACAGGGAAC CACGGGACGT GCGGTTAGGG 
GACATTATGA AAAATTTAGG AAATATTTAT GAGCCTACAG TAATTGAAGG AAAGCAGAAC 
TTGCATATTA TCCCCGATTC TCGTCAAGTG AATCCAGAGG ATCTTCAAGC TGGCATTTGT 
TACTTGCAAA TTACATCTGT CGATCCTGTT ACAGAGGACG AGGATTTGGA AAGCAGGCGA 
GAAAGACAAT CCGGGAGGTC CTCGTCAACA TTCAGTGCAC GAGTCTTCGA TCGATTTTTA 
TTTGACACTC CTTTCACAAA GAATGGAAGA TCCCAAGGAG GTTTGGAAGA TCAGTGGAAG 
AGGCGTACGG TTTTGTGGAC TGAAGGGCCT TTTCCATCTC TTGTAAATCG TTTAATGGTT 
GTGAAGTCTG AGTCGCGAGA ATTTTCGCCA ATCGAAAACG CCATAGGAAT GATTGAGACT 
CGTACAGCAG CTTTAGCAGG AGAGCTCGAT GATCAACGTA TAAATGAGGG AGATCCTCTG 
CCCCGTTTAC AAGGCCTGCA AAGAATCTTG CAAGGAAGTG TTGCAGTCCA GGTAAATAGT 
GGAGTGCTAG GTGTCTGCAC AGCTTTCTTG TCTGGGGAAC CTTCAACACG ATTGCGCTCA 
CAAGAACTAC AGCAGCTGAT AGCAGCACTC TTGGAGTTTA TGGCTGTGTG CAAGAAAGCA 
ATTCGAGTAC ATGCACGACT GATTGGAGAG GAGGACCAAG ACTTCCACTC GCAGCTTGTG 
ATTGGATTCC AATCACTCAC AGCAGAGCTA TCCCACTATA TTCCCGCCAT CTTGTCAGAT 
GTAACCATAC TTGGGAAACA CAGCAATGTG CTCAAGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024369372.1
CDS	348..6269
Translation	MHLVAGAPIWSVESVKEGKGSRFDVSDSVGQCRVGSHDRRDEEETWGLNAETESSAFWELSFETRAVVDSGSIKKQGMDEIALSGQRFKRIPHPYIGQSTDLESLLNGNGDPFPHFKELLQSYRAEWVKDEFKYGHYEAVPPPALEHQIFEGPDTDIQTEFRLSNVRLGRNGDVTDDEDPQTPRRLSRRFSVRSSGRFSMRSTTSDAPEALHTNISKHYGVAPLPPYEPVYNWRATRASVLGQRTSELPSAISSSGLKVSVKVVSLNVQAGLVEPMYGTLCLYHREKREKLSENFHFRCLPSEFQDCVTWQEGGNCLRRAIFSLEAASPAICLLIQLEKHVTEEGGVTAHVYSRKEPVHLTEREKQKLQVWARVMPFREPFAWAVVALFDANITGGVGGFSSSPSSPLPPGLFGPSLMEASLDSDGRLIADAKTESSSVPVLVDVQSLRRVKENYTEEMLQDPKYKVHKPVKAILRLEIERLSTEDHDQDSIFECGSFGRGSTDGDSRLTVGPSFSGRFMQGLGMTKVTHNGRQKWSPGRKHRYLHSGSVGSIEFGKFDFRAIEFRSGSKVEVLPQLLHCLYVYPQSVNLSRKRNLFVRVELRKDDVDIRKSPLEALYPRDADSSMQKYAHSQIDANTKTPHYHDEFKIQLPAVLSPEHHLLFTFFHVDLQMKIEAPKPVVVGYSVLPLMFVCQVQRLDGSLPIAKELYSNYLQENVKERLEYLDDGKTVFKLRSRLCSSLYPVNERIRDFFSEYDRHVLRTSPPWGTELMEAINALKTVEPSAMLQFLQPILNMLLRMIRDGGETLQVAAFRSMVNIITRVQAETSDGAERNRYLVHYVDYAFNDFGGRHEPVYPGLCSVWRSLARSKAKGYRVGPVYDDVLSMAWVFLELIVKSMALEQSRIYSESLPPGEELPPLQLIDEVFKSIVQLYDCLLTEVHERCKKGMLLAMKLNSSIAYFCYDLLSVIEHRQVFELVALYFNKFAGVCQSLVHECKLNFLRIVCDHDLFVEMPGRDPTERNYLASLLMQELFVTWDHEDLTLRSKAARILVQLMCKHEYDARYQTLEDKMYIAQRYFPLVDLILDEMPVFYGLSTTEKREVLVCVLHILRYLDDASLVKAWQHNMDRTKLFFKLLEECQELLEYKKAGADGLMGAMPANDQGEGPLRYSEKLSPAVNQFLIEASRQDVRLTKISPNAQWILPQTSPEIGSFWKRASPVTNSSTSPGQPHSLREALAQAQSAGRSGGRSLRETLHPMLRQKLELWEENLSASAMLQILEVLEKFISAVSSQAVLTDYMRLDCITTIFIGFLGRSQPLPFWKAFLPFFNNVFRRHGATLMSRENDRFLKQVAFHLLRLGVFRNESIRKRSVVGLQILVRNAYHYFQGLSRLRVMLTITLSELMSDVQVTHPKMDGSLEESGESQRLRRSLQQIAQESISADLLRECGLPEDSLTALPEGVNGQQWSWAGVAELSTSLLKAVDAAVGHALLGPEILMNDKYATAEAYHGLATAYTNVPDLHIMWLLYLCEVHQGNQSYAEAAQCAVAVAGVIMHAIVGKGDHVWGKEHVEALGNIFPMLTGSRFGDAATAEIEGYGSSKLTVESAVKYLQLANKLFVQAELFHFCAGILELIIPVHKSRRAYSQLSKCHTSLTSIYEAILEQESSPIPFTDATYYRVGFYGKSFGSLHCKEYVYREPRDVRLGDIMKNLGNIYEPTVIEGKQNLHIIPDSRQVNPEDLQAGICYLQITSVDPVTEDEDLESRRERQSGRSSSTFSARVFDRFLFDTPFTKNGRSQGGLEDQWKRRTVLWTEGPFPSLVNRLMVVKSESREFSPIENAIGMIETRTAALAGELDDQRINEGDPLPRLQGLQRILQGSVAVQVNSGVLGVCTAFLSGEPSTRLRSQELQQLIAALLEFMAVCKKAIRVHARLIGEEDQDFHSQLVIGFQSLTAELSHYIPAILSDVTILGKHSNVLKL

Target ORF information:

RefSeq Version	XM_024513604.1
Organism	Physcomitrella patens
Definition	Physcomitrella patens guanine nucleotide exchange factor SPIKE 1-like (LOC112279271), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024513604.1

ORF Insert Sequence:

ATGCATTTGG TCGCTGGAGC GCCTATATGG TCGGTGGAAA GCGTCAAGGA GGGAAAAGGA 
TCCAGGTTCG ACGTGTCGGA CTCAGTAGGA CAATGTAGGG TAGGTAGTCA TGATCGGAGG 
GACGAGGAGG AGACATGGGG ACTCAATGCA GAGACAGAAA GCTCTGCATT CTGGGAGCTG 
AGCTTCGAGA CTAGAGCTGT CGTCGACAGC GGATCAATTA AGAAGCAGGG GATGGATGAA 
ATTGCCTTGA GCGGGCAGCG GTTCAAACGT ATCCCACACC CGTACATCGG GCAGTCAACA 
GATCTTGAGT CCTTGCTGAA TGGGAATGGA GACCCTTTCC CACATTTCAA AGAGCTTCTT 
CAAAGCTACA GGGCTGAATG GGTGAAGGAT GAGTTCAAAT ACGGTCATTA TGAAGCTGTG 
CCACCGCCAG CCTTAGAACA TCAGATTTTT GAAGGACCCG ACACTGACAT ACAGACAGAG 
TTTCGGCTTT CTAACGTCAG ACTCGGCAGA AATGGTGATG TTACTGATGA TGAAGATCCT 
CAAACTCCAC GACGGCTTTC AAGACGTTTT TCTGTACGGT CATCTGGTCG TTTCTCTATG 
AGATCTACGA CTAGTGATGC ACCTGAAGCA TTGCACACAA ATATCTCAAA GCATTATGGG 
GTTGCTCCGT TACCTCCTTA TGAGCCCGTT TATAATTGGC GAGCAACAAG GGCATCTGTA 
CTTGGACAAA GAACTTCGGA ACTTCCATCA GCAATATCAT CTAGCGGGCT GAAGGTTTCT 
GTGAAGGTTG TCTCTTTGAA TGTTCAGGCC GGACTTGTCG AACCCATGTA CGGTACCTTG 
TGTCTCTATC ATCGAGAGAA GCGGGAGAAA TTATCAGAAA ATTTTCACTT CCGATGTTTA 
CCATCAGAGT TCCAAGATTG TGTAACTTGG CAGGAGGGTG GTAATTGTCT GAGAAGAGCT 
ATATTTTCAC TGGAGGCAGC GTCGCCAGCA ATATGTTTGT TGATCCAGCT TGAAAAGCAT 
GTGACTGAGG AAGGAGGAGT AACGGCTCAT GTTTATTCTC GAAAAGAACC TGTACACTTG 
ACCGAGAGAG AAAAGCAGAA GTTACAGGTC TGGGCGCGCG TGATGCCATT CAGGGAGCCT 
TTCGCGTGGG CAGTAGTTGC TCTGTTTGAT GCAAATATAA CAGGAGGTGT AGGAGGATTC 
TCATCTTCTC CAAGCAGCCC ACTTCCACCA GGACTTTTTG GACCATCTTT GATGGAAGCA 
TCCTTGGATT CTGATGGACG ACTTATAGCT GATGCTAAGA CAGAATCATC TAGTGTGCCA 
GTTTTGGTTG ATGTTCAGAG CCTTCGCCGG GTCAAAGAAA ATTACACGGA GGAAATGCTT 
CAGGATCCCA AGTACAAAGT GCACAAACCC GTGAAGGCCA TCCTTCGTCT CGAGATAGAG 
AGGTTATCTA CTGAAGATCA TGACCAAGAT TCCATATTTG AGTGTGGTAG TTTTGGTCGT 
GGGTCCACTG ATGGTGATTC CCGCTTGACA GTAGGACCCT CTTTCTCAGG GCGTTTCATG 
CAGGGGCTGG GCATGACGAA GGTTACGCAT AATGGGCGGC AGAAATGGAG CCCTGGAAGA 
AAACATCGCT ATCTGCATTC CGGCAGTGTT GGCAGCATTG AATTTGGAAA GTTTGACTTC 
CGGGCTATTG AGTTTCGATC GGGTTCGAAA GTTGAGGTTT TACCTCAGCT ATTGCACTGC 
CTGTATGTAT ATCCTCAATC AGTCAATTTA AGTCGAAAAC GCAATCTTTT TGTGCGAGTG 
GAGCTTCGAA AGGATGATGT AGACATTCGC AAATCTCCTC TCGAGGCCCT TTATCCGAGG 
GATGCTGACA GCTCTATGCA GAAATATGCA CACTCTCAGA TCGATGCAAA CACCAAGACA 
CCTCACTATC ATGATGAGTT CAAAATTCAA CTACCTGCCG TTCTCTCTCC TGAGCATCAC 
CTTCTGTTCA CGTTTTTTCA TGTGGATCTA CAGATGAAAA TTGAGGCTCC AAAGCCGGTG 
GTAGTGGGAT ACTCTGTTCT TCCGCTCATG TTTGTTTGTC AGGTTCAGCG GTTGGACGGC 
AGCCTACCTA TCGCAAAGGA GCTGTATTCC AATTATCTAC AAGAAAATGT GAAGGAAAGG 
TTGGAGTACT TGGATGATGG CAAAACTGTA TTTAAATTGA GGTCACGATT ATGCTCTTCT 
CTTTATCCGG TTAATGAGAG GATCCGAGAT TTCTTTTCAG AATACGATCG ACATGTCTTG 
CGGACCAGTC CACCCTGGGG TACTGAGCTG ATGGAGGCTA TTAATGCTCT GAAGACGGTT 
GAGCCGTCAG CTATGCTCCA GTTTCTTCAA CCTATTCTCA ACATGCTGCT TCGTATGATT 
CGCGATGGAG GAGAAACGTT GCAGGTTGCT GCATTCCGTT CAATGGTTAA CATCATTACC 
AGGGTACAAG CAGAGACATC TGATGGGGCT GAAAGAAATC GTTACCTTGT GCATTATGTG 
GATTATGCAT TTAATGATTT TGGTGGGCGT CATGAGCCTG TGTACCCAGG TCTGTGCAGT 
GTATGGCGCA GCCTTGCTCG AAGCAAGGCG AAAGGCTACC GTGTCGGACC TGTGTATGAT 
GATGTTCTTT CCATGGCTTG GGTGTTCCTG GAGCTAATTG TCAAGTCCAT GGCACTTGAA 
CAATCCCGCA TTTACTCTGA GTCCCTCCCA CCTGGAGAGG AGTTACCACC TTTGCAGCTC 
ATTGATGAGG TCTTTAAAAG TATCGTACAG CTCTATGATT GTTTACTGAC AGAGGTTCAT 
GAGCGCTGCA AGAAGGGCAT GCTTCTCGCA ATGAAGCTGA ATAGCAGTAT TGCATACTTT 
TGCTATGACC TTCTCTCTGT AATTGAGCAT CGACAAGTCT TTGAGCTGGT TGCGCTTTAT 
TTCAACAAGT TTGCTGGGGT ATGTCAATCT CTTGTGCACG AATGTAAGCT TAATTTTCTG 
CGCATCGTCT GTGACCATGA TCTCTTTGTT GAGATGCCCG GTCGAGATCC TACAGAGAGG 
AATTATTTGG CATCACTGTT GATGCAAGAG CTCTTTGTTA CTTGGGATCA TGAAGATCTG 
ACTTTAAGAT CTAAGGCAGC TCGAATCCTT GTGCAACTTA TGTGCAAGCA TGAGTATGAT 
GCACGGTATC AAACATTGGA GGACAAAATG TATATTGCCC AACGTTACTT TCCACTAGTT 
GACCTGATAT TGGACGAAAT GCCAGTGTTC TATGGCCTGA GCACTACAGA GAAGCGTGAA 
GTGCTGGTGT GTGTGCTTCA CATTCTGCGA TATCTAGACG ATGCATCTCT TGTTAAAGCT 
TGGCAGCACA ACATGGATAG AACAAAGCTT TTTTTCAAAC TCCTGGAAGA ATGTCAGGAA 
TTGCTGGAGT ATAAGAAGGC TGGAGCAGAT GGCTTGATGG GAGCTATGCC AGCGAATGAT 
CAAGGAGAAG GGCCCCTTCG ATACTCCGAG AAGCTCTCAC CTGCAGTCAA TCAGTTTTTG 
ATTGAGGCAT CTCGGCAGGA TGTCAGACTC ACAAAGATTT CTCCAAATGC TCAGTGGATA 
CTTCCACAAA CGAGTCCAGA GATTGGTTCA TTCTGGAAAA GAGCAAGCCC TGTAACAAAT 
AGTTCAACTA GCCCAGGTCA GCCGCATTCG TTGCGAGAGG CACTAGCCCA AGCACAGTCT 
GCGGGAAGAA GTGGAGGCAG GTCTCTTCGT GAGACCTTGC ATCCTATGCT CCGCCAAAAG 
TTGGAGCTAT GGGAGGAGAA CCTTAGTGCC TCTGCTATGC TCCAAATTTT GGAGGTACTT 
GAGAAGTTTA TAAGTGCAGT ATCAAGTCAA GCTGTTCTCA CTGACTACAT GAGGCTTGAT 
TGTATCACAA CTATTTTTAT TGGTTTTCTG GGACGCAGCC AGCCGTTGCC ATTTTGGAAG 
GCCTTTCTAC CTTTCTTCAA TAACGTGTTT CGTCGTCATG GCGCTACCTT GATGAGTAGG 
GAGAATGATA GGTTCTTGAA GCAAGTAGCA TTTCATCTAC TACGGCTCGG AGTTTTCCGG 
AATGAGTCCA TACGGAAACG TTCTGTTGTT GGCCTTCAAA TTCTTGTCCG AAATGCATAC 
CACTACTTTC AAGGCCTTTC AAGACTGCGG GTGATGCTTA CAATCACACT TTCTGAGCTC 
ATGTCAGATG TTCAAGTAAC ACATCCTAAG ATGGATGGAT CCTTGGAAGA GAGTGGTGAA 
TCTCAACGTC TTCGTAGGTC CTTGCAACAG ATTGCACAAG AAAGTATTAG TGCGGATCTT 
CTACGGGAAT GTGGTCTTCC TGAAGATTCT CTTACTGCTT TGCCAGAAGG TGTGAATGGC 
CAACAATGGA GTTGGGCTGG AGTCGCTGAG CTTTCCACCT CTCTGCTGAA AGCTGTGGAT 
GCTGCTGTAG GACATGCTCT TTTGGGTCCT GAGATTTTAA TGAATGACAA GTATGCAACT 
GCAGAGGCCT ACCATGGTTT GGCGACGGCA TATACCAACG TGCCCGATCT GCATATAATG 
TGGCTTCTGT ATCTCTGCGA AGTTCATCAA GGCAACCAGT CTTATGCAGA GGCTGCTCAG 
TGTGCTGTTG CGGTTGCAGG TGTCATCATG CATGCTATAG TAGGAAAAGG TGATCATGTT 
TGGGGAAAAG AGCATGTTGA GGCACTGGGT AATATTTTCC CAATGCTCAC TGGTTCCCGC 
TTTGGGGATG CTGCAACTGC CGAAATTGAA GGCTATGGGT CATCGAAATT GACTGTCGAA 
TCGGCAGTGA AGTACTTGCA GCTCGCCAAT AAACTCTTTG TCCAGGCAGA GCTCTTCCAT 
TTCTGTGCCG GCATATTGGA GCTCATAATT CCCGTCCATA AGTCAAGGCG TGCCTATAGT 
CAGCTGTCTA AATGTCATAC TTCATTGACC AGCATCTACG AAGCTATACT AGAGCAAGAG 
TCGAGCCCTA TCCCTTTCAC GGATGCAACA TATTATCGTG TGGGATTTTA TGGGAAAAGC 
TTTGGTAGTT TGCACTGCAA GGAGTATGTG TACAGGGAAC CACGGGACGT GCGGTTAGGG 
GACATTATGA AAAATTTAGG AAATATTTAT GAGCCTACAG TAATTGAAGG AAAGCAGAAC 
TTGCATATTA TCCCCGATTC TCGTCAAGTG AATCCAGAGG ATCTTCAAGC TGGCATTTGT 
TACTTGCAAA TTACATCTGT CGATCCTGTT ACAGAGGACG AGGATTTGGA AAGCAGGCGA 
GAAAGACAAT CCGGGAGGTC CTCGTCAACA TTCAGTGCAC GAGTCTTCGA TCGATTTTTA 
TTTGACACTC CTTTCACAAA GAATGGAAGA TCCCAAGGAG GTTTGGAAGA TCAGTGGAAG 
AGGCGTACGG TTTTGTGGAC TGAAGGGCCT TTTCCATCTC TTGTAAATCG TTTAATGGTT 
GTGAAGTCTG AGTCGCGAGA ATTTTCGCCA ATCGAAAACG CCATAGGAAT GATTGAGACT 
CGTACAGCAG CTTTAGCAGG AGAGCTCGAT GATCAACGTA TAAATGAGGG AGATCCTCTG 
CCCCGTTTAC AAGGCCTGCA AAGAATCTTG CAAGGAAGTG TTGCAGTCCA GGTAAATAGT 
GGAGTGCTAG GTGTCTGCAC AGCTTTCTTG TCTGGGGAAC CTTCAACACG ATTGCGCTCA 
CAAGAACTAC AGCAGCTGAT AGCAGCACTC TTGGAGTTTA TGGCTGTGTG CAAGAAAGCA 
ATTCGAGTAC ATGCACGACT GATTGGAGAG GAGGACCAAG ACTTCCACTC GCAGCTTGTG 
ATTGGATTCC AATCACTCAC AGCAGAGCTA TCCCACTATA TTCCCGCCAT CTTGTCAGAT 
GTAACCATAC TTGGGAAACA CAGCAATGTG CTCAAGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC112279271 ( XM_024513613.1 ) cDNA ORF clone, -> XP_024369381.1 Physcomitrella patens guanine nucleotide exchange factor SPIKE 1-like (LOC112279271), transcript variant X10, mRNA.

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CloneID	OPn41283
Clone ID Related Accession (Same CDS sequence)	XM_024513613.1
Accession Version	XM_024513613.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Physcomitrella patens
Product	guanine nucleotide exchange factor SPIKE 1-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037253.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physcomitrella patens Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATTTGG TCGCTGGAGC GCCTATATGG TCGGTGGAAA GCGTCAAGGA GGGAAAAGGA 
TCCAGGTTCG ACGTGTCGGA CTCAGTAGGA CAATGTAGGG TAGGTAGTCA TGATCGGAGG 
GACGAGGAGG AGACATGGGG ACTCAATGCA GAGACAGAAA GCTCTGCATT CTGGGAGCTG 
AGCTTCGAGA CTAGAGCTGT CGTCGACAGC GGATCAATTA AGAAGCAGGG GATGGATGAA 
ATTGCCTTGA GCGGGCAGCG GTTCAAACGT ATCCCACACC CGTACATCGG GCAGTCAACA 
GATCTTGAGT CCTTGCTGAA TGGGAATGGA GACCCTTTCC CACATTTCAA AGAGCTTCTT 
CAAAGCTACA GGGCTGAATG GGTGAAGGAT GAGTTCAAAT ACGGTCATTA TGAAGCTGTG 
CCACCGCCAG CCTTAGAACA TCAGATTTTT GAAGGACCCG ACACTGACAT ACAGACAGAG 
TTTCGGCTTT CTAACGTCAG ACTCGGCAGA AATGGTGATG TTACTGATGA TGAAGATCCT 
CAAACTCCAC GACGGCTTTC AAGACGTTTT TCTGTACGGT CATCTGGTCG TTTCTCTATG 
AGATCTACGA CTAGTGATGC ACCTGAAGCA TTGCACACAA ATATCTCAAA GCATTATGGG 
GTTGCTCCGT TACCTCCTTA TGAGCCCGTT TATAATTGGC GAGCAACAAG GGCATCTGTA 
CTTGGACAAA GAACTTCGGA ACTTCCATCA GCAATATCAT CTAGTAGCGG GCTGAAGGTT 
TCTGTGAAGG TTGTCTCTTT GAATGTTCAG GCCGGACTTG TCGAACCCAT GTACGGTACC 
TTGTGTCTCT ATCATCGAGA GAAGCGGGAG AAATTATCAG AAAATTTTCA CTTCCGATGT 
TTACCATCAG AGTTCCAAGA TGAGGGTGGT AATTGTCTGA GAAGAGCTAT ATTTTCACTG 
GAGGCAGCGT CGCCAGCAAT ATGTTTGTTG ATCCAGCTTG AAAAGCATGT GACTGAGGAA 
GGAGGAGTAA CGGCTCATGT TTATTCTCGA AAAGAACCTG TACACTTGAC CGAGAGAGAA 
AAGCAGAAGT TACAGGTCTG GGCGCGCGTG ATGCCATTCA GGGAGCCTTT CGCGTGGGCA 
GTAGTTGCTC TGTTTGATGC AAATATAACA GGAGGTGTAG GAGGATTCTC ATCTTCTCCA 
AGCAGCCCAC TTCCACCAGG ACTTTTTGGA CCATCTTTGA TGGAAGCATC CTTGGATTCT 
GATGGACGAC TTATAGCTGA TGCTAAGACA GAATCATCTA GTGTGCCAGT TTTGGTTGAT 
GTTCAGAGCC TTCGCCGGGT CAAAGAAAAT TACACGGAGG AAATGCTTCA GGATCCCAAG 
TACAAAGTGC ACAAACCCGT GAAGGCCATC CTTCGTCTCG AGATAGAGAG GTTATCTACT 
GAAGATCATG ACCAAGATTC CATATTTGAG TGTGGTAGTT TTGGTCGTGG GTCCACTGAT 
GGTGATTCCC GCTTGACAGT AGGACCCTCT TTCTCAGGGC GTTTCATGCA GGGGCTGGGC 
ATGACGAAGG TTACGCATAA TGGGCGGCAG AAATGGAGCC CTGGAAGAAA ACATCGCTAT 
CTGCATTCCG GCAGTGTTGG CAGCATTGAA TTTGGAAAGT TTGACTTCCG GGCTATTGAG 
TTTCGATCGG GTTCGAAAGT TGAGGTTTTA CCTCAGCTAT TGCACTGCCT GTATGTATAT 
CCTCAATCAG TCAATTTAAG TCGAAAACGC AATCTTTTTG TGCGAGTGGA GCTTCGAAAG 
GATGATGTAG ACATTCGCAA ATCTCCTCTC GAGGCCCTTT ATCCGAGGGA TGCTGACAGC 
TCTATGCAGA AATATGCACA CTCTCAGATC GATGCAAACA CCAAGACACC TCACTATCAT 
GATGAGTTCA AAATTCAACT ACCTGCCGTT CTCTCTCCTG AGCATCACCT TCTGTTCACG 
TTTTTTCATG TGGATCTACA GATGAAAATT GAGGCTCCAA AGCCGGTGGT AGTGGGATAC 
TCTGTTCTTC CGCTCATGTT TGTTTGTCAG GTTCAGCGGT TGGACGGCAG CCTACCTATC 
GCAAAGGAGC TGTATTCCAA TTATCTACAA GAAAATGTGA AGGAAAGGTT GGAGTACTTG 
GATGATGGCA AAACTGTATT TAAATTGAGG TCACGATTAT GCTCTTCTCT TTATCCGGTT 
AATGAGAGGA TCCGAGATTT CTTTTCAGAA TACGATCGAC ATGTCTTGCG GACCAGTCCA 
CCCTGGGGTA CTGAGCTGAT GGAGGCTATT AATGCTCTGA AGACGGTTGA GCCGTCAGCT 
ATGCTCCAGT TTCTTCAACC TATTCTCAAC ATGCTGCTTC GTATGATTCG CGATGGAGGA 
GAAACGTTGC AGGTTGCTGC ATTCCGTTCA ATGGTTAACA TCATTACCAG GGTACAAGCA 
GAGACATCTG ATGGGGCTGA AAGAAATCGT TACCTTGTGC ATTATGTGGA TTATGCATTT 
AATGATTTTG GTGGGCGTCA TGAGCCTGTG TACCCAGGTC TGTGCAGTGT ATGGCGCAGC 
CTTGCTCGAA GCAAGGCGAA AGGCTACCGT GTCGGACCTG TGTATGATGA TGTTCTTTCC 
ATGGCTTGGG TGTTCCTGGA GCTAATTGTC AAGTCCATGG CACTTGAACA ATCCCGCATT 
TACTCTGAGT CCCTCCCACC TGGAGAGGAG TTACCACCTT TGCAGCTCAT TGATGAGGTC 
TTTAAAAGTA TCGTACAGCT CTATGATTGT TTACTGACAG AGGTTCATGA GCGCTGCAAG 
AAGGGCATGC TTCTCGCAAT GAAGCTGAAT AGCAGTATTG CATACTTTTG CTATGACCTT 
CTCTCTGTAA TTGAGCATCG ACAAGTCTTT GAGCTGGTTG CGCTTTATTT CAACAAGTTT 
GCTGGGGTAT GTCAATCTCT TGTGCACGAA TGTAAGCTTA ATTTTCTGCG CATCGTCTGT 
GACCATGATC TCTTTGTTGA GATGCCCGGT CGAGATCCTA CAGAGAGGAA TTATTTGGCA 
TCACTGTTGA TGCAAGAGCT CTTTGTTACT TGGGATCATG AAGATCTGAC TTTAAGATCT 
AAGGCAGCTC GAATCCTTGT GCAACTTATG TGCAAGCATG AGTATGATGC ACGGTATCAA 
ACATTGGAGG ACAAAATGTA TATTGCCCAA CGTTACTTTC CACTAGTTGA CCTGATATTG 
GACGAAATGC CAGTGTTCTA TGGCCTGAGC ACTACAGAGA AGCGTGAAGT GCTGGTGTGT 
GTGCTTCACA TTCTGCGATA TCTAGACGAT GCATCTCTTG TTAAAGCTTG GCAGCACAAC 
ATGGATAGAA CAAAGCTTTT TTTCAAACTC CTGGAAGAAT GTCAGGAATT GCTGGAGTAT 
AAGAAGGCTG GAGCAGATGG CTTGATGGGA GCTATGCCAG CGAATGATCA AGGAGAAGGG 
CCCCTTCGAT ACTCCGAGAA GCTCTCACCT GCAGTCAATC AGTTTTTGAT TGAGGCATCT 
CGGCAGGATG TCAGACTCAC AAAGATTTCT CCAAATGCTC AGTGGATACT TCCACAAACG 
AGTCCAGAGA TTGGTTCATT CTGGAAAAGA GCAAGCCCTG TAACAAATAG TTCAACTAGC 
CCAGGTCAGC CGCATTCGTT GCGAGAGGCA CTAGCCCAAG CACAGTCTGC GGGAAGAAGT 
GGAGGCAGGT CTCTTCGTGA GACCTTGCAT CCTATGCTCC GCCAAAAGTT GGAGCTATGG 
GAGGAGAACC TTAGTGCCTC TGCTATGCTC CAAATTTTGG AGGTACTTGA GAAGTTTATA 
AGTGCAGTAT CAAGTCAAGC TGTTCTCACT GACTACATGA GGCTTGATTG TATCACAACT 
ATTTTTATTG GTTTTCTGGG ACGCAGCCAG CCGTTGCCAT TTTGGAAGGC CTTTCTACCT 
TTCTTCAATA ACGTGTTTCG TCGTCATGGC GCTACCTTGA TGAGTAGGGA GAATGATAGG 
TTCTTGAAGC AAGTAGCATT TCATCTACTA CGGCTCGGAG TTTTCCGGAA TGAGTCCATA 
CGGAAACGTT CTGTTGTTGG CCTTCAAATT CTTGTCCGAA ATGCATACCA CTACTTTCAA 
GGCCTTTCAA GACTGCGGGT GATGCTTACA ATCACACTTT CTGAGCTCAT GTCAGATGTT 
CAAGTAACAC ATCCTAAGAT GGATGGATCC TTGGAAGAGA GTGGTGAATC TCAACGTCTT 
CGTAGGTCCT TGCAACAGAT TGCACAAGAA AGTATTAGTG CGGATCTTCT ACGGGAATGT 
GGTCTTCCTG AAGATTCTCT TACTGCTTTG CCAGAAGGTG TGAATGGCCA ACAATGGAGT 
TGGGCTGGAG TCGCTGAGCT TTCCACCTCT CTGCTGAAAG CTGTGGATGC TGCTGTAGGA 
CATGCTCTTT TGGGTCCTGA GATTTTAATG AATGACAAGT ATGCAACTGC AGAGGCCTAC 
CATGGTTTGG CGACGGCATA TACCAACGTG CCCGATCTGC ATATAATGTG GCTTCTGTAT 
CTCTGCGAAG TTCATCAAGG CAACCAGTCT TATGCAGAGG CTGCTCAGTG TGCTGTTGCG 
GTTGCAGGTG TCATCATGCA TGCTATAGTA GGAAAAGGTG ATCATGTTTG GGGAAAAGAG 
CATGTTGAGG CACTGGGTAA TATTTTCCCA ATGCTCACTG GTTCCCGCTT TGGGGATGCT 
GCAACTGCCG AAATTGAAGG CTATGGGTCA TCGAAATTGA CTGTCGAATC GGCAGTGAAG 
TACTTGCAGC TCGCCAATAA ACTCTTTGTC CAGGCAGAGC TCTTCCATTT CTGTGCCGGC 
ATATTGGAGC TCATAATTCC CGTCCATAAG TCAAGGCGTG CCTATAGTCA GCTGTCTAAA 
TGTCATACTT CATTGACCAG CATCTACGAA GCTATACTAG AGCAAGAGTC GAGCCCTATC 
CCTTTCACGG ATGCAACATA TTATCGTGTG GGATTTTATG GGAAAAGCTT TGGTAGTTTG 
CACTGCAAGG AGTATGTGTA CAGGGAACCA CGGGACGTGC GGTTAGGGGA CATTATGAAA 
AATTTAGGAA ATATTTATGA GCCTACAGTA ATTGAAGGAA AGCAGAACTT GCATATTATC 
CCCGATTCTC GTCAAGTGAA TCCAGAGGAT CTTCAAGCTG GCATTTGTTA CTTGCAAATT 
ACATCTGTCG ATCCTGTTAC AGAGGACGAG GATTTGGAAA GCAGGCGAGA AAGACAATCC 
GGGAGGTCCT CGTCAACATT CAGTGCACGA GTCTTCGATC GATTTTTATT TGACACTCCT 
TTCACAAAGA ATGGAAGATC CCAAGGAGGT TTGGAAGATC AGTGGAAGAG GCGTACGGTT 
TTGTGGACTG AAGGGCCTTT TCCATCTCTT GTAAATCGTT TAATGGTTGT GAAGTCTGAG 
TCGCGAGAAT TTTCGCCAAT CGAAAACGCC ATAGGAATGA TTGAGACTCG TACAGCAGCT 
TTAGCAGGAG AGCTCGATGA TCAACGTATA AATGAGGGAG ATCCTCTGCC CCGTTTACAA 
GGCCTGCAAA GAATCTTGCA AGGAAGTGTT GCAGTCCAGG TAAATAGTGG AGTGCTAGGT 
GTCTGCACAG CTTTCTTGTC TGGGGAACCT TCAACACGAT TGCGCTCACA AGAACTACAG 
CAGCTGATAG CAGCACTCTT GGAGTTTATG GCTGTGTGCA AGAAAGCAAT TCGAGTACAT 
GCACGACTGA TTGGAGAGGA GGACCAAGAC TTCCACTCGC AGCTTGTGAT TGGATTCCAA 
TCACTCACAG CAGAGCTATC CCACTATATT CCCGCCATCT TGTCAGATGT AACCATACTT 
GGGAAACACA GCAATGTGCT CAAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024369381.1
CDS	347..6256
Translation	MHLVAGAPIWSVESVKEGKGSRFDVSDSVGQCRVGSHDRRDEEETWGLNAETESSAFWELSFETRAVVDSGSIKKQGMDEIALSGQRFKRIPHPYIGQSTDLESLLNGNGDPFPHFKELLQSYRAEWVKDEFKYGHYEAVPPPALEHQIFEGPDTDIQTEFRLSNVRLGRNGDVTDDEDPQTPRRLSRRFSVRSSGRFSMRSTTSDAPEALHTNISKHYGVAPLPPYEPVYNWRATRASVLGQRTSELPSAISSSSGLKVSVKVVSLNVQAGLVEPMYGTLCLYHREKREKLSENFHFRCLPSEFQDEGGNCLRRAIFSLEAASPAICLLIQLEKHVTEEGGVTAHVYSRKEPVHLTEREKQKLQVWARVMPFREPFAWAVVALFDANITGGVGGFSSSPSSPLPPGLFGPSLMEASLDSDGRLIADAKTESSSVPVLVDVQSLRRVKENYTEEMLQDPKYKVHKPVKAILRLEIERLSTEDHDQDSIFECGSFGRGSTDGDSRLTVGPSFSGRFMQGLGMTKVTHNGRQKWSPGRKHRYLHSGSVGSIEFGKFDFRAIEFRSGSKVEVLPQLLHCLYVYPQSVNLSRKRNLFVRVELRKDDVDIRKSPLEALYPRDADSSMQKYAHSQIDANTKTPHYHDEFKIQLPAVLSPEHHLLFTFFHVDLQMKIEAPKPVVVGYSVLPLMFVCQVQRLDGSLPIAKELYSNYLQENVKERLEYLDDGKTVFKLRSRLCSSLYPVNERIRDFFSEYDRHVLRTSPPWGTELMEAINALKTVEPSAMLQFLQPILNMLLRMIRDGGETLQVAAFRSMVNIITRVQAETSDGAERNRYLVHYVDYAFNDFGGRHEPVYPGLCSVWRSLARSKAKGYRVGPVYDDVLSMAWVFLELIVKSMALEQSRIYSESLPPGEELPPLQLIDEVFKSIVQLYDCLLTEVHERCKKGMLLAMKLNSSIAYFCYDLLSVIEHRQVFELVALYFNKFAGVCQSLVHECKLNFLRIVCDHDLFVEMPGRDPTERNYLASLLMQELFVTWDHEDLTLRSKAARILVQLMCKHEYDARYQTLEDKMYIAQRYFPLVDLILDEMPVFYGLSTTEKREVLVCVLHILRYLDDASLVKAWQHNMDRTKLFFKLLEECQELLEYKKAGADGLMGAMPANDQGEGPLRYSEKLSPAVNQFLIEASRQDVRLTKISPNAQWILPQTSPEIGSFWKRASPVTNSSTSPGQPHSLREALAQAQSAGRSGGRSLRETLHPMLRQKLELWEENLSASAMLQILEVLEKFISAVSSQAVLTDYMRLDCITTIFIGFLGRSQPLPFWKAFLPFFNNVFRRHGATLMSRENDRFLKQVAFHLLRLGVFRNESIRKRSVVGLQILVRNAYHYFQGLSRLRVMLTITLSELMSDVQVTHPKMDGSLEESGESQRLRRSLQQIAQESISADLLRECGLPEDSLTALPEGVNGQQWSWAGVAELSTSLLKAVDAAVGHALLGPEILMNDKYATAEAYHGLATAYTNVPDLHIMWLLYLCEVHQGNQSYAEAAQCAVAVAGVIMHAIVGKGDHVWGKEHVEALGNIFPMLTGSRFGDAATAEIEGYGSSKLTVESAVKYLQLANKLFVQAELFHFCAGILELIIPVHKSRRAYSQLSKCHTSLTSIYEAILEQESSPIPFTDATYYRVGFYGKSFGSLHCKEYVYREPRDVRLGDIMKNLGNIYEPTVIEGKQNLHIIPDSRQVNPEDLQAGICYLQITSVDPVTEDEDLESRRERQSGRSSSTFSARVFDRFLFDTPFTKNGRSQGGLEDQWKRRTVLWTEGPFPSLVNRLMVVKSESREFSPIENAIGMIETRTAALAGELDDQRINEGDPLPRLQGLQRILQGSVAVQVNSGVLGVCTAFLSGEPSTRLRSQELQQLIAALLEFMAVCKKAIRVHARLIGEEDQDFHSQLVIGFQSLTAELSHYIPAILSDVTILGKHSNVLKL

Target ORF information:

RefSeq Version	XM_024513613.1
Organism	Physcomitrella patens
Definition	Physcomitrella patens guanine nucleotide exchange factor SPIKE 1-like (LOC112279271), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024513613.1

ORF Insert Sequence:

ATGCATTTGG TCGCTGGAGC GCCTATATGG TCGGTGGAAA GCGTCAAGGA GGGAAAAGGA 
TCCAGGTTCG ACGTGTCGGA CTCAGTAGGA CAATGTAGGG TAGGTAGTCA TGATCGGAGG 
GACGAGGAGG AGACATGGGG ACTCAATGCA GAGACAGAAA GCTCTGCATT CTGGGAGCTG 
AGCTTCGAGA CTAGAGCTGT CGTCGACAGC GGATCAATTA AGAAGCAGGG GATGGATGAA 
ATTGCCTTGA GCGGGCAGCG GTTCAAACGT ATCCCACACC CGTACATCGG GCAGTCAACA 
GATCTTGAGT CCTTGCTGAA TGGGAATGGA GACCCTTTCC CACATTTCAA AGAGCTTCTT 
CAAAGCTACA GGGCTGAATG GGTGAAGGAT GAGTTCAAAT ACGGTCATTA TGAAGCTGTG 
CCACCGCCAG CCTTAGAACA TCAGATTTTT GAAGGACCCG ACACTGACAT ACAGACAGAG 
TTTCGGCTTT CTAACGTCAG ACTCGGCAGA AATGGTGATG TTACTGATGA TGAAGATCCT 
CAAACTCCAC GACGGCTTTC AAGACGTTTT TCTGTACGGT CATCTGGTCG TTTCTCTATG 
AGATCTACGA CTAGTGATGC ACCTGAAGCA TTGCACACAA ATATCTCAAA GCATTATGGG 
GTTGCTCCGT TACCTCCTTA TGAGCCCGTT TATAATTGGC GAGCAACAAG GGCATCTGTA 
CTTGGACAAA GAACTTCGGA ACTTCCATCA GCAATATCAT CTAGTAGCGG GCTGAAGGTT 
TCTGTGAAGG TTGTCTCTTT GAATGTTCAG GCCGGACTTG TCGAACCCAT GTACGGTACC 
TTGTGTCTCT ATCATCGAGA GAAGCGGGAG AAATTATCAG AAAATTTTCA CTTCCGATGT 
TTACCATCAG AGTTCCAAGA TGAGGGTGGT AATTGTCTGA GAAGAGCTAT ATTTTCACTG 
GAGGCAGCGT CGCCAGCAAT ATGTTTGTTG ATCCAGCTTG AAAAGCATGT GACTGAGGAA 
GGAGGAGTAA CGGCTCATGT TTATTCTCGA AAAGAACCTG TACACTTGAC CGAGAGAGAA 
AAGCAGAAGT TACAGGTCTG GGCGCGCGTG ATGCCATTCA GGGAGCCTTT CGCGTGGGCA 
GTAGTTGCTC TGTTTGATGC AAATATAACA GGAGGTGTAG GAGGATTCTC ATCTTCTCCA 
AGCAGCCCAC TTCCACCAGG ACTTTTTGGA CCATCTTTGA TGGAAGCATC CTTGGATTCT 
GATGGACGAC TTATAGCTGA TGCTAAGACA GAATCATCTA GTGTGCCAGT TTTGGTTGAT 
GTTCAGAGCC TTCGCCGGGT CAAAGAAAAT TACACGGAGG AAATGCTTCA GGATCCCAAG 
TACAAAGTGC ACAAACCCGT GAAGGCCATC CTTCGTCTCG AGATAGAGAG GTTATCTACT 
GAAGATCATG ACCAAGATTC CATATTTGAG TGTGGTAGTT TTGGTCGTGG GTCCACTGAT 
GGTGATTCCC GCTTGACAGT AGGACCCTCT TTCTCAGGGC GTTTCATGCA GGGGCTGGGC 
ATGACGAAGG TTACGCATAA TGGGCGGCAG AAATGGAGCC CTGGAAGAAA ACATCGCTAT 
CTGCATTCCG GCAGTGTTGG CAGCATTGAA TTTGGAAAGT TTGACTTCCG GGCTATTGAG 
TTTCGATCGG GTTCGAAAGT TGAGGTTTTA CCTCAGCTAT TGCACTGCCT GTATGTATAT 
CCTCAATCAG TCAATTTAAG TCGAAAACGC AATCTTTTTG TGCGAGTGGA GCTTCGAAAG 
GATGATGTAG ACATTCGCAA ATCTCCTCTC GAGGCCCTTT ATCCGAGGGA TGCTGACAGC 
TCTATGCAGA AATATGCACA CTCTCAGATC GATGCAAACA CCAAGACACC TCACTATCAT 
GATGAGTTCA AAATTCAACT ACCTGCCGTT CTCTCTCCTG AGCATCACCT TCTGTTCACG 
TTTTTTCATG TGGATCTACA GATGAAAATT GAGGCTCCAA AGCCGGTGGT AGTGGGATAC 
TCTGTTCTTC CGCTCATGTT TGTTTGTCAG GTTCAGCGGT TGGACGGCAG CCTACCTATC 
GCAAAGGAGC TGTATTCCAA TTATCTACAA GAAAATGTGA AGGAAAGGTT GGAGTACTTG 
GATGATGGCA AAACTGTATT TAAATTGAGG TCACGATTAT GCTCTTCTCT TTATCCGGTT 
AATGAGAGGA TCCGAGATTT CTTTTCAGAA TACGATCGAC ATGTCTTGCG GACCAGTCCA 
CCCTGGGGTA CTGAGCTGAT GGAGGCTATT AATGCTCTGA AGACGGTTGA GCCGTCAGCT 
ATGCTCCAGT TTCTTCAACC TATTCTCAAC ATGCTGCTTC GTATGATTCG CGATGGAGGA 
GAAACGTTGC AGGTTGCTGC ATTCCGTTCA ATGGTTAACA TCATTACCAG GGTACAAGCA 
GAGACATCTG ATGGGGCTGA AAGAAATCGT TACCTTGTGC ATTATGTGGA TTATGCATTT 
AATGATTTTG GTGGGCGTCA TGAGCCTGTG TACCCAGGTC TGTGCAGTGT ATGGCGCAGC 
CTTGCTCGAA GCAAGGCGAA AGGCTACCGT GTCGGACCTG TGTATGATGA TGTTCTTTCC 
ATGGCTTGGG TGTTCCTGGA GCTAATTGTC AAGTCCATGG CACTTGAACA ATCCCGCATT 
TACTCTGAGT CCCTCCCACC TGGAGAGGAG TTACCACCTT TGCAGCTCAT TGATGAGGTC 
TTTAAAAGTA TCGTACAGCT CTATGATTGT TTACTGACAG AGGTTCATGA GCGCTGCAAG 
AAGGGCATGC TTCTCGCAAT GAAGCTGAAT AGCAGTATTG CATACTTTTG CTATGACCTT 
CTCTCTGTAA TTGAGCATCG ACAAGTCTTT GAGCTGGTTG CGCTTTATTT CAACAAGTTT 
GCTGGGGTAT GTCAATCTCT TGTGCACGAA TGTAAGCTTA ATTTTCTGCG CATCGTCTGT 
GACCATGATC TCTTTGTTGA GATGCCCGGT CGAGATCCTA CAGAGAGGAA TTATTTGGCA 
TCACTGTTGA TGCAAGAGCT CTTTGTTACT TGGGATCATG AAGATCTGAC TTTAAGATCT 
AAGGCAGCTC GAATCCTTGT GCAACTTATG TGCAAGCATG AGTATGATGC ACGGTATCAA 
ACATTGGAGG ACAAAATGTA TATTGCCCAA CGTTACTTTC CACTAGTTGA CCTGATATTG 
GACGAAATGC CAGTGTTCTA TGGCCTGAGC ACTACAGAGA AGCGTGAAGT GCTGGTGTGT 
GTGCTTCACA TTCTGCGATA TCTAGACGAT GCATCTCTTG TTAAAGCTTG GCAGCACAAC 
ATGGATAGAA CAAAGCTTTT TTTCAAACTC CTGGAAGAAT GTCAGGAATT GCTGGAGTAT 
AAGAAGGCTG GAGCAGATGG CTTGATGGGA GCTATGCCAG CGAATGATCA AGGAGAAGGG 
CCCCTTCGAT ACTCCGAGAA GCTCTCACCT GCAGTCAATC AGTTTTTGAT TGAGGCATCT 
CGGCAGGATG TCAGACTCAC AAAGATTTCT CCAAATGCTC AGTGGATACT TCCACAAACG 
AGTCCAGAGA TTGGTTCATT CTGGAAAAGA GCAAGCCCTG TAACAAATAG TTCAACTAGC 
CCAGGTCAGC CGCATTCGTT GCGAGAGGCA CTAGCCCAAG CACAGTCTGC GGGAAGAAGT 
GGAGGCAGGT CTCTTCGTGA GACCTTGCAT CCTATGCTCC GCCAAAAGTT GGAGCTATGG 
GAGGAGAACC TTAGTGCCTC TGCTATGCTC CAAATTTTGG AGGTACTTGA GAAGTTTATA 
AGTGCAGTAT CAAGTCAAGC TGTTCTCACT GACTACATGA GGCTTGATTG TATCACAACT 
ATTTTTATTG GTTTTCTGGG ACGCAGCCAG CCGTTGCCAT TTTGGAAGGC CTTTCTACCT 
TTCTTCAATA ACGTGTTTCG TCGTCATGGC GCTACCTTGA TGAGTAGGGA GAATGATAGG 
TTCTTGAAGC AAGTAGCATT TCATCTACTA CGGCTCGGAG TTTTCCGGAA TGAGTCCATA 
CGGAAACGTT CTGTTGTTGG CCTTCAAATT CTTGTCCGAA ATGCATACCA CTACTTTCAA 
GGCCTTTCAA GACTGCGGGT GATGCTTACA ATCACACTTT CTGAGCTCAT GTCAGATGTT 
CAAGTAACAC ATCCTAAGAT GGATGGATCC TTGGAAGAGA GTGGTGAATC TCAACGTCTT 
CGTAGGTCCT TGCAACAGAT TGCACAAGAA AGTATTAGTG CGGATCTTCT ACGGGAATGT 
GGTCTTCCTG AAGATTCTCT TACTGCTTTG CCAGAAGGTG TGAATGGCCA ACAATGGAGT 
TGGGCTGGAG TCGCTGAGCT TTCCACCTCT CTGCTGAAAG CTGTGGATGC TGCTGTAGGA 
CATGCTCTTT TGGGTCCTGA GATTTTAATG AATGACAAGT ATGCAACTGC AGAGGCCTAC 
CATGGTTTGG CGACGGCATA TACCAACGTG CCCGATCTGC ATATAATGTG GCTTCTGTAT 
CTCTGCGAAG TTCATCAAGG CAACCAGTCT TATGCAGAGG CTGCTCAGTG TGCTGTTGCG 
GTTGCAGGTG TCATCATGCA TGCTATAGTA GGAAAAGGTG ATCATGTTTG GGGAAAAGAG 
CATGTTGAGG CACTGGGTAA TATTTTCCCA ATGCTCACTG GTTCCCGCTT TGGGGATGCT 
GCAACTGCCG AAATTGAAGG CTATGGGTCA TCGAAATTGA CTGTCGAATC GGCAGTGAAG 
TACTTGCAGC TCGCCAATAA ACTCTTTGTC CAGGCAGAGC TCTTCCATTT CTGTGCCGGC 
ATATTGGAGC TCATAATTCC CGTCCATAAG TCAAGGCGTG CCTATAGTCA GCTGTCTAAA 
TGTCATACTT CATTGACCAG CATCTACGAA GCTATACTAG AGCAAGAGTC GAGCCCTATC 
CCTTTCACGG ATGCAACATA TTATCGTGTG GGATTTTATG GGAAAAGCTT TGGTAGTTTG 
CACTGCAAGG AGTATGTGTA CAGGGAACCA CGGGACGTGC GGTTAGGGGA CATTATGAAA 
AATTTAGGAA ATATTTATGA GCCTACAGTA ATTGAAGGAA AGCAGAACTT GCATATTATC 
CCCGATTCTC GTCAAGTGAA TCCAGAGGAT CTTCAAGCTG GCATTTGTTA CTTGCAAATT 
ACATCTGTCG ATCCTGTTAC AGAGGACGAG GATTTGGAAA GCAGGCGAGA AAGACAATCC 
GGGAGGTCCT CGTCAACATT CAGTGCACGA GTCTTCGATC GATTTTTATT TGACACTCCT 
TTCACAAAGA ATGGAAGATC CCAAGGAGGT TTGGAAGATC AGTGGAAGAG GCGTACGGTT 
TTGTGGACTG AAGGGCCTTT TCCATCTCTT GTAAATCGTT TAATGGTTGT GAAGTCTGAG 
TCGCGAGAAT TTTCGCCAAT CGAAAACGCC ATAGGAATGA TTGAGACTCG TACAGCAGCT 
TTAGCAGGAG AGCTCGATGA TCAACGTATA AATGAGGGAG ATCCTCTGCC CCGTTTACAA 
GGCCTGCAAA GAATCTTGCA AGGAAGTGTT GCAGTCCAGG TAAATAGTGG AGTGCTAGGT 
GTCTGCACAG CTTTCTTGTC TGGGGAACCT TCAACACGAT TGCGCTCACA AGAACTACAG 
CAGCTGATAG CAGCACTCTT GGAGTTTATG GCTGTGTGCA AGAAAGCAAT TCGAGTACAT 
GCACGACTGA TTGGAGAGGA GGACCAAGAC TTCCACTCGC AGCTTGTGAT TGGATTCCAA 
TCACTCACAG CAGAGCTATC CCACTATATT CCCGCCATCT TGTCAGATGT AACCATACTT 
GGGAAACACA GCAATGTGCT CAAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC112279271 ( XM_024513623.1 ) cDNA ORF clone, -> XP_024369391.1 Physcomitrella patens guanine nucleotide exchange factor SPIKE 1-like (LOC112279271), transcript variant X11, mRNA.

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CloneID	OPn41284
Clone ID Related Accession (Same CDS sequence)	XM_024513623.1
Accession Version	XM_024513623.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Physcomitrella patens
Product	guanine nucleotide exchange factor SPIKE 1-like isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037253.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physcomitrella patens Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATTTGG TCGCTGGAGC GCCTATATGG TCGGTGGAAA GCGTCAAGGA GGGAAAAGGA 
TCCAGGTTCG ACGTGTCGGA CTCAGTAGGA CAATGTAGGG TAGGTAGTCA TGATCGGAGG 
GACGAGGAGG AGACATGGGG ACTCAATGCA GAGACAGAAA GCTCTGCATT CTGGGAGCTG 
AGCTTCGAGA CTAGAGCTGT CGTCGACAGC GGATCAATTA AGAAGCAGGG GATGGATGAA 
ATTGCCTTGA GCGGGCAGCG GTTCAAACGT ATCCCACACC CGTACATCGG GCAGTCAACA 
GATCTTGAGT CCTTGCTGAA TGGGAATGGA GACCCTTTCC CACATTTCAA AGAGCTTCTT 
CAAAGCTACA GGGCTGAATG GGTGAAGGAT GAGTTCAAAT ACGGTCATTA TGAAGCTGTG 
CCACCGCCAG CCTTAGAACA TCAGATTTTT GAAGGACCCG ACACTGACAT ACAGACAGAG 
TTTCGGCTTT CTAACGTCAG ACTCGGCAGA AATGGTGATG TTACTGATGA TGAAGATCCT 
CAAACTCCAC GACGGCTTTC AAGACGTTTT TCTGTACGGT CATCTGGTCG TTTCTCTATG 
AGATCTACGA CTAGTGATGC ACCTGAAGCA TTGCACACAA ATATCTCAAA GCATTATGGG 
GTTGCTCCGT TACCTCCTTA TGAGCCCGTT TATAATTGGC GAGCAACAAG GGCATCTGTA 
CTTGGACAAA GAACTTCGGA ACTTCCATCA GCAATATCAT CTAGCGGGCT GAAGGTTTCT 
GTGAAGGTTG TCTCTTTGAA TGTTCAGGCC GGACTTGTCG AACCCATGTA CGGTACCTTG 
TGTCTCTATC ATCGAGAGAA GCGGGAGAAA TTATCAGAAA ATTTTCACTT CCGATGTTTA 
CCATCAGAGT TCCAAGATGA GGGTGGTAAT TGTCTGAGAA GAGCTATATT TTCACTGGAG 
GCAGCGTCGC CAGCAATATG TTTGTTGATC CAGCTTGAAA AGCATGTGAC TGAGGAAGGA 
GGAGTAACGG CTCATGTTTA TTCTCGAAAA GAACCTGTAC ACTTGACCGA GAGAGAAAAG 
CAGAAGTTAC AGGTCTGGGC GCGCGTGATG CCATTCAGGG AGCCTTTCGC GTGGGCAGTA 
GTTGCTCTGT TTGATGCAAA TATAACAGGA GGTGTAGGAG GATTCTCATC TTCTCCAAGC 
AGCCCACTTC CACCAGGACT TTTTGGACCA TCTTTGATGG AAGCATCCTT GGATTCTGAT 
GGACGACTTA TAGCTGATGC TAAGACAGAA TCATCTAGTG TGCCAGTTTT GGTTGATGTT 
CAGAGCCTTC GCCGGGTCAA AGAAAATTAC ACGGAGGAAA TGCTTCAGGA TCCCAAGTAC 
AAAGTGCACA AACCCGTGAA GGCCATCCTT CGTCTCGAGA TAGAGAGGTT ATCTACTGAA 
GATCATGACC AAGATTCCAT ATTTGAGTGT GGTAGTTTTG GTCGTGGGTC CACTGATGGT 
GATTCCCGCT TGACAGTAGG ACCCTCTTTC TCAGGGCGTT TCATGCAGGG GCTGGGCATG 
ACGAAGGTTA CGCATAATGG GCGGCAGAAA TGGAGCCCTG GAAGAAAACA TCGCTATCTG 
CATTCCGGCA GTGTTGGCAG CATTGAATTT GGAAAGTTTG ACTTCCGGGC TATTGAGTTT 
CGATCGGGTT CGAAAGTTGA GGTTTTACCT CAGCTATTGC ACTGCCTGTA TGTATATCCT 
CAATCAGTCA ATTTAAGTCG AAAACGCAAT CTTTTTGTGC GAGTGGAGCT TCGAAAGGAT 
GATGTAGACA TTCGCAAATC TCCTCTCGAG GCCCTTTATC CGAGGGATGC TGACAGCTCT 
ATGCAGAAAT ATGCACACTC TCAGATCGAT GCAAACACCA AGACACCTCA CTATCATGAT 
GAGTTCAAAA TTCAACTACC TGCCGTTCTC TCTCCTGAGC ATCACCTTCT GTTCACGTTT 
TTTCATGTGG ATCTACAGAT GAAAATTGAG GCTCCAAAGC CGGTGGTAGT GGGATACTCT 
GTTCTTCCGC TCATGTTTGT TTGTCAGGTT CAGCGGTTGG ACGGCAGCCT ACCTATCGCA 
AAGGAGCTGT ATTCCAATTA TCTACAAGAA AATGTGAAGG AAAGGTTGGA GTACTTGGAT 
GATGGCAAAA CTGTATTTAA ATTGAGGTCA CGATTATGCT CTTCTCTTTA TCCGGTTAAT 
GAGAGGATCC GAGATTTCTT TTCAGAATAC GATCGACATG TCTTGCGGAC CAGTCCACCC 
TGGGGTACTG AGCTGATGGA GGCTATTAAT GCTCTGAAGA CGGTTGAGCC GTCAGCTATG 
CTCCAGTTTC TTCAACCTAT TCTCAACATG CTGCTTCGTA TGATTCGCGA TGGAGGAGAA 
ACGTTGCAGG TTGCTGCATT CCGTTCAATG GTTAACATCA TTACCAGGGT ACAAGCAGAG 
ACATCTGATG GGGCTGAAAG AAATCGTTAC CTTGTGCATT ATGTGGATTA TGCATTTAAT 
GATTTTGGTG GGCGTCATGA GCCTGTGTAC CCAGGTCTGT GCAGTGTATG GCGCAGCCTT 
GCTCGAAGCA AGGCGAAAGG CTACCGTGTC GGACCTGTGT ATGATGATGT TCTTTCCATG 
GCTTGGGTGT TCCTGGAGCT AATTGTCAAG TCCATGGCAC TTGAACAATC CCGCATTTAC 
TCTGAGTCCC TCCCACCTGG AGAGGAGTTA CCACCTTTGC AGCTCATTGA TGAGGTCTTT 
AAAAGTATCG TACAGCTCTA TGATTGTTTA CTGACAGAGG TTCATGAGCG CTGCAAGAAG 
GGCATGCTTC TCGCAATGAA GCTGAATAGC AGTATTGCAT ACTTTTGCTA TGACCTTCTC 
TCTGTAATTG AGCATCGACA AGTCTTTGAG CTGGTTGCGC TTTATTTCAA CAAGTTTGCT 
GGGGTATGTC AATCTCTTGT GCACGAATGT AAGCTTAATT TTCTGCGCAT CGTCTGTGAC 
CATGATCTCT TTGTTGAGAT GCCCGGTCGA GATCCTACAG AGAGGAATTA TTTGGCATCA 
CTGTTGATGC AAGAGCTCTT TGTTACTTGG GATCATGAAG ATCTGACTTT AAGATCTAAG 
GCAGCTCGAA TCCTTGTGCA ACTTATGTGC AAGCATGAGT ATGATGCACG GTATCAAACA 
TTGGAGGACA AAATGTATAT TGCCCAACGT TACTTTCCAC TAGTTGACCT GATATTGGAC 
GAAATGCCAG TGTTCTATGG CCTGAGCACT ACAGAGAAGC GTGAAGTGCT GGTGTGTGTG 
CTTCACATTC TGCGATATCT AGACGATGCA TCTCTTGTTA AAGCTTGGCA GCACAACATG 
GATAGAACAA AGCTTTTTTT CAAACTCCTG GAAGAATGTC AGGAATTGCT GGAGTATAAG 
AAGGCTGGAG CAGATGGCTT GATGGGAGCT ATGCCAGCGA ATGATCAAGG AGAAGGGCCC 
CTTCGATACT CCGAGAAGCT CTCACCTGCA GTCAATCAGT TTTTGATTGA GGCATCTCGG 
CAGGATGTCA GACTCACAAA GATTTCTCCA AATGCTCAGT GGATACTTCC ACAAACGAGT 
CCAGAGATTG GTTCATTCTG GAAAAGAGCA AGCCCTGTAA CAAATAGTTC AACTAGCCCA 
GGTCAGCCGC ATTCGTTGCG AGAGGCACTA GCCCAAGCAC AGTCTGCGGG AAGAAGTGGA 
GGCAGGTCTC TTCGTGAGAC CTTGCATCCT ATGCTCCGCC AAAAGTTGGA GCTATGGGAG 
GAGAACCTTA GTGCCTCTGC TATGCTCCAA ATTTTGGAGG TACTTGAGAA GTTTATAAGT 
GCAGTATCAA GTCAAGCTGT TCTCACTGAC TACATGAGGC TTGATTGTAT CACAACTATT 
TTTATTGGTT TTCTGGGACG CAGCCAGCCG TTGCCATTTT GGAAGGCCTT TCTACCTTTC 
TTCAATAACG TGTTTCGTCG TCATGGCGCT ACCTTGATGA GTAGGGAGAA TGATAGGTTC 
TTGAAGCAAG TAGCATTTCA TCTACTACGG CTCGGAGTTT TCCGGAATGA GTCCATACGG 
AAACGTTCTG TTGTTGGCCT TCAAATTCTT GTCCGAAATG CATACCACTA CTTTCAAGGC 
CTTTCAAGAC TGCGGGTGAT GCTTACAATC ACACTTTCTG AGCTCATGTC AGATGTTCAA 
GTAACACATC CTAAGATGGA TGGATCCTTG GAAGAGAGTG GTGAATCTCA ACGTCTTCGT 
AGGTCCTTGC AACAGATTGC ACAAGAAAGT ATTAGTGCGG ATCTTCTACG GGAATGTGGT 
CTTCCTGAAG ATTCTCTTAC TGCTTTGCCA GAAGGTGTGA ATGGCCAACA ATGGAGTTGG 
GCTGGAGTCG CTGAGCTTTC CACCTCTCTG CTGAAAGCTG TGGATGCTGC TGTAGGACAT 
GCTCTTTTGG GTCCTGAGAT TTTAATGAAT GACAAGTATG CAACTGCAGA GGCCTACCAT 
GGTTTGGCGA CGGCATATAC CAACGTGCCC GATCTGCATA TAATGTGGCT TCTGTATCTC 
TGCGAAGTTC ATCAAGGCAA CCAGTCTTAT GCAGAGGCTG CTCAGTGTGC TGTTGCGGTT 
GCAGGTGTCA TCATGCATGC TATAGTAGGA AAAGGTGATC ATGTTTGGGG AAAAGAGCAT 
GTTGAGGCAC TGGGTAATAT TTTCCCAATG CTCACTGGTT CCCGCTTTGG GGATGCTGCA 
ACTGCCGAAA TTGAAGGCTA TGGGTCATCG AAATTGACTG TCGAATCGGC AGTGAAGTAC 
TTGCAGCTCG CCAATAAACT CTTTGTCCAG GCAGAGCTCT TCCATTTCTG TGCCGGCATA 
TTGGAGCTCA TAATTCCCGT CCATAAGTCA AGGCGTGCCT ATAGTCAGCT GTCTAAATGT 
CATACTTCAT TGACCAGCAT CTACGAAGCT ATACTAGAGC AAGAGTCGAG CCCTATCCCT 
TTCACGGATG CAACATATTA TCGTGTGGGA TTTTATGGGA AAAGCTTTGG TAGTTTGCAC 
TGCAAGGAGT ATGTGTACAG GGAACCACGG GACGTGCGGT TAGGGGACAT TATGAAAAAT 
TTAGGAAATA TTTATGAGCC TACAGTAATT GAAGGAAAGC AGAACTTGCA TATTATCCCC 
GATTCTCGTC AAGTGAATCC AGAGGATCTT CAAGCTGGCA TTTGTTACTT GCAAATTACA 
TCTGTCGATC CTGTTACAGA GGACGAGGAT TTGGAAAGCA GGCGAGAAAG ACAATCCGGG 
AGGTCCTCGT CAACATTCAG TGCACGAGTC TTCGATCGAT TTTTATTTGA CACTCCTTTC 
ACAAAGAATG GAAGATCCCA AGGAGGTTTG GAAGATCAGT GGAAGAGGCG TACGGTTTTG 
TGGACTGAAG GGCCTTTTCC ATCTCTTGTA AATCGTTTAA TGGTTGTGAA GTCTGAGTCG 
CGAGAATTTT CGCCAATCGA AAACGCCATA GGAATGATTG AGACTCGTAC AGCAGCTTTA 
GCAGGAGAGC TCGATGATCA ACGTATAAAT GAGGGAGATC CTCTGCCCCG TTTACAAGGC 
CTGCAAAGAA TCTTGCAAGG AAGTGTTGCA GTCCAGGTAA ATAGTGGAGT GCTAGGTGTC 
TGCACAGCTT TCTTGTCTGG GGAACCTTCA ACACGATTGC GCTCACAAGA ACTACAGCAG 
CTGATAGCAG CACTCTTGGA GTTTATGGCT GTGTGCAAGA AAGCAATTCG AGTACATGCA 
CGACTGATTG GAGAGGAGGA CCAAGACTTC CACTCGCAGC TTGTGATTGG ATTCCAATCA 
CTCACAGCAG AGCTATCCCA CTATATTCCC GCCATCTTGT CAGATGTAAC CATACTTGGG 
AAACACAGCA ATGTGCTCAA GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024369391.1
CDS	347..6253
Translation	MHLVAGAPIWSVESVKEGKGSRFDVSDSVGQCRVGSHDRRDEEETWGLNAETESSAFWELSFETRAVVDSGSIKKQGMDEIALSGQRFKRIPHPYIGQSTDLESLLNGNGDPFPHFKELLQSYRAEWVKDEFKYGHYEAVPPPALEHQIFEGPDTDIQTEFRLSNVRLGRNGDVTDDEDPQTPRRLSRRFSVRSSGRFSMRSTTSDAPEALHTNISKHYGVAPLPPYEPVYNWRATRASVLGQRTSELPSAISSSGLKVSVKVVSLNVQAGLVEPMYGTLCLYHREKREKLSENFHFRCLPSEFQDEGGNCLRRAIFSLEAASPAICLLIQLEKHVTEEGGVTAHVYSRKEPVHLTEREKQKLQVWARVMPFREPFAWAVVALFDANITGGVGGFSSSPSSPLPPGLFGPSLMEASLDSDGRLIADAKTESSSVPVLVDVQSLRRVKENYTEEMLQDPKYKVHKPVKAILRLEIERLSTEDHDQDSIFECGSFGRGSTDGDSRLTVGPSFSGRFMQGLGMTKVTHNGRQKWSPGRKHRYLHSGSVGSIEFGKFDFRAIEFRSGSKVEVLPQLLHCLYVYPQSVNLSRKRNLFVRVELRKDDVDIRKSPLEALYPRDADSSMQKYAHSQIDANTKTPHYHDEFKIQLPAVLSPEHHLLFTFFHVDLQMKIEAPKPVVVGYSVLPLMFVCQVQRLDGSLPIAKELYSNYLQENVKERLEYLDDGKTVFKLRSRLCSSLYPVNERIRDFFSEYDRHVLRTSPPWGTELMEAINALKTVEPSAMLQFLQPILNMLLRMIRDGGETLQVAAFRSMVNIITRVQAETSDGAERNRYLVHYVDYAFNDFGGRHEPVYPGLCSVWRSLARSKAKGYRVGPVYDDVLSMAWVFLELIVKSMALEQSRIYSESLPPGEELPPLQLIDEVFKSIVQLYDCLLTEVHERCKKGMLLAMKLNSSIAYFCYDLLSVIEHRQVFELVALYFNKFAGVCQSLVHECKLNFLRIVCDHDLFVEMPGRDPTERNYLASLLMQELFVTWDHEDLTLRSKAARILVQLMCKHEYDARYQTLEDKMYIAQRYFPLVDLILDEMPVFYGLSTTEKREVLVCVLHILRYLDDASLVKAWQHNMDRTKLFFKLLEECQELLEYKKAGADGLMGAMPANDQGEGPLRYSEKLSPAVNQFLIEASRQDVRLTKISPNAQWILPQTSPEIGSFWKRASPVTNSSTSPGQPHSLREALAQAQSAGRSGGRSLRETLHPMLRQKLELWEENLSASAMLQILEVLEKFISAVSSQAVLTDYMRLDCITTIFIGFLGRSQPLPFWKAFLPFFNNVFRRHGATLMSRENDRFLKQVAFHLLRLGVFRNESIRKRSVVGLQILVRNAYHYFQGLSRLRVMLTITLSELMSDVQVTHPKMDGSLEESGESQRLRRSLQQIAQESISADLLRECGLPEDSLTALPEGVNGQQWSWAGVAELSTSLLKAVDAAVGHALLGPEILMNDKYATAEAYHGLATAYTNVPDLHIMWLLYLCEVHQGNQSYAEAAQCAVAVAGVIMHAIVGKGDHVWGKEHVEALGNIFPMLTGSRFGDAATAEIEGYGSSKLTVESAVKYLQLANKLFVQAELFHFCAGILELIIPVHKSRRAYSQLSKCHTSLTSIYEAILEQESSPIPFTDATYYRVGFYGKSFGSLHCKEYVYREPRDVRLGDIMKNLGNIYEPTVIEGKQNLHIIPDSRQVNPEDLQAGICYLQITSVDPVTEDEDLESRRERQSGRSSSTFSARVFDRFLFDTPFTKNGRSQGGLEDQWKRRTVLWTEGPFPSLVNRLMVVKSESREFSPIENAIGMIETRTAALAGELDDQRINEGDPLPRLQGLQRILQGSVAVQVNSGVLGVCTAFLSGEPSTRLRSQELQQLIAALLEFMAVCKKAIRVHARLIGEEDQDFHSQLVIGFQSLTAELSHYIPAILSDVTILGKHSNVLKL

Target ORF information:

RefSeq Version	XM_024513623.1
Organism	Physcomitrella patens
Definition	Physcomitrella patens guanine nucleotide exchange factor SPIKE 1-like (LOC112279271), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024513623.1

ORF Insert Sequence:

ATGCATTTGG TCGCTGGAGC GCCTATATGG TCGGTGGAAA GCGTCAAGGA GGGAAAAGGA 
TCCAGGTTCG ACGTGTCGGA CTCAGTAGGA CAATGTAGGG TAGGTAGTCA TGATCGGAGG 
GACGAGGAGG AGACATGGGG ACTCAATGCA GAGACAGAAA GCTCTGCATT CTGGGAGCTG 
AGCTTCGAGA CTAGAGCTGT CGTCGACAGC GGATCAATTA AGAAGCAGGG GATGGATGAA 
ATTGCCTTGA GCGGGCAGCG GTTCAAACGT ATCCCACACC CGTACATCGG GCAGTCAACA 
GATCTTGAGT CCTTGCTGAA TGGGAATGGA GACCCTTTCC CACATTTCAA AGAGCTTCTT 
CAAAGCTACA GGGCTGAATG GGTGAAGGAT GAGTTCAAAT ACGGTCATTA TGAAGCTGTG 
CCACCGCCAG CCTTAGAACA TCAGATTTTT GAAGGACCCG ACACTGACAT ACAGACAGAG 
TTTCGGCTTT CTAACGTCAG ACTCGGCAGA AATGGTGATG TTACTGATGA TGAAGATCCT 
CAAACTCCAC GACGGCTTTC AAGACGTTTT TCTGTACGGT CATCTGGTCG TTTCTCTATG 
AGATCTACGA CTAGTGATGC ACCTGAAGCA TTGCACACAA ATATCTCAAA GCATTATGGG 
GTTGCTCCGT TACCTCCTTA TGAGCCCGTT TATAATTGGC GAGCAACAAG GGCATCTGTA 
CTTGGACAAA GAACTTCGGA ACTTCCATCA GCAATATCAT CTAGCGGGCT GAAGGTTTCT 
GTGAAGGTTG TCTCTTTGAA TGTTCAGGCC GGACTTGTCG AACCCATGTA CGGTACCTTG 
TGTCTCTATC ATCGAGAGAA GCGGGAGAAA TTATCAGAAA ATTTTCACTT CCGATGTTTA 
CCATCAGAGT TCCAAGATGA GGGTGGTAAT TGTCTGAGAA GAGCTATATT TTCACTGGAG 
GCAGCGTCGC CAGCAATATG TTTGTTGATC CAGCTTGAAA AGCATGTGAC TGAGGAAGGA 
GGAGTAACGG CTCATGTTTA TTCTCGAAAA GAACCTGTAC ACTTGACCGA GAGAGAAAAG 
CAGAAGTTAC AGGTCTGGGC GCGCGTGATG CCATTCAGGG AGCCTTTCGC GTGGGCAGTA 
GTTGCTCTGT TTGATGCAAA TATAACAGGA GGTGTAGGAG GATTCTCATC TTCTCCAAGC 
AGCCCACTTC CACCAGGACT TTTTGGACCA TCTTTGATGG AAGCATCCTT GGATTCTGAT 
GGACGACTTA TAGCTGATGC TAAGACAGAA TCATCTAGTG TGCCAGTTTT GGTTGATGTT 
CAGAGCCTTC GCCGGGTCAA AGAAAATTAC ACGGAGGAAA TGCTTCAGGA TCCCAAGTAC 
AAAGTGCACA AACCCGTGAA GGCCATCCTT CGTCTCGAGA TAGAGAGGTT ATCTACTGAA 
GATCATGACC AAGATTCCAT ATTTGAGTGT GGTAGTTTTG GTCGTGGGTC CACTGATGGT 
GATTCCCGCT TGACAGTAGG ACCCTCTTTC TCAGGGCGTT TCATGCAGGG GCTGGGCATG 
ACGAAGGTTA CGCATAATGG GCGGCAGAAA TGGAGCCCTG GAAGAAAACA TCGCTATCTG 
CATTCCGGCA GTGTTGGCAG CATTGAATTT GGAAAGTTTG ACTTCCGGGC TATTGAGTTT 
CGATCGGGTT CGAAAGTTGA GGTTTTACCT CAGCTATTGC ACTGCCTGTA TGTATATCCT 
CAATCAGTCA ATTTAAGTCG AAAACGCAAT CTTTTTGTGC GAGTGGAGCT TCGAAAGGAT 
GATGTAGACA TTCGCAAATC TCCTCTCGAG GCCCTTTATC CGAGGGATGC TGACAGCTCT 
ATGCAGAAAT ATGCACACTC TCAGATCGAT GCAAACACCA AGACACCTCA CTATCATGAT 
GAGTTCAAAA TTCAACTACC TGCCGTTCTC TCTCCTGAGC ATCACCTTCT GTTCACGTTT 
TTTCATGTGG ATCTACAGAT GAAAATTGAG GCTCCAAAGC CGGTGGTAGT GGGATACTCT 
GTTCTTCCGC TCATGTTTGT TTGTCAGGTT CAGCGGTTGG ACGGCAGCCT ACCTATCGCA 
AAGGAGCTGT ATTCCAATTA TCTACAAGAA AATGTGAAGG AAAGGTTGGA GTACTTGGAT 
GATGGCAAAA CTGTATTTAA ATTGAGGTCA CGATTATGCT CTTCTCTTTA TCCGGTTAAT 
GAGAGGATCC GAGATTTCTT TTCAGAATAC GATCGACATG TCTTGCGGAC CAGTCCACCC 
TGGGGTACTG AGCTGATGGA GGCTATTAAT GCTCTGAAGA CGGTTGAGCC GTCAGCTATG 
CTCCAGTTTC TTCAACCTAT TCTCAACATG CTGCTTCGTA TGATTCGCGA TGGAGGAGAA 
ACGTTGCAGG TTGCTGCATT CCGTTCAATG GTTAACATCA TTACCAGGGT ACAAGCAGAG 
ACATCTGATG GGGCTGAAAG AAATCGTTAC CTTGTGCATT ATGTGGATTA TGCATTTAAT 
GATTTTGGTG GGCGTCATGA GCCTGTGTAC CCAGGTCTGT GCAGTGTATG GCGCAGCCTT 
GCTCGAAGCA AGGCGAAAGG CTACCGTGTC GGACCTGTGT ATGATGATGT TCTTTCCATG 
GCTTGGGTGT TCCTGGAGCT AATTGTCAAG TCCATGGCAC TTGAACAATC CCGCATTTAC 
TCTGAGTCCC TCCCACCTGG AGAGGAGTTA CCACCTTTGC AGCTCATTGA TGAGGTCTTT 
AAAAGTATCG TACAGCTCTA TGATTGTTTA CTGACAGAGG TTCATGAGCG CTGCAAGAAG 
GGCATGCTTC TCGCAATGAA GCTGAATAGC AGTATTGCAT ACTTTTGCTA TGACCTTCTC 
TCTGTAATTG AGCATCGACA AGTCTTTGAG CTGGTTGCGC TTTATTTCAA CAAGTTTGCT 
GGGGTATGTC AATCTCTTGT GCACGAATGT AAGCTTAATT TTCTGCGCAT CGTCTGTGAC 
CATGATCTCT TTGTTGAGAT GCCCGGTCGA GATCCTACAG AGAGGAATTA TTTGGCATCA 
CTGTTGATGC AAGAGCTCTT TGTTACTTGG GATCATGAAG ATCTGACTTT AAGATCTAAG 
GCAGCTCGAA TCCTTGTGCA ACTTATGTGC AAGCATGAGT ATGATGCACG GTATCAAACA 
TTGGAGGACA AAATGTATAT TGCCCAACGT TACTTTCCAC TAGTTGACCT GATATTGGAC 
GAAATGCCAG TGTTCTATGG CCTGAGCACT ACAGAGAAGC GTGAAGTGCT GGTGTGTGTG 
CTTCACATTC TGCGATATCT AGACGATGCA TCTCTTGTTA AAGCTTGGCA GCACAACATG 
GATAGAACAA AGCTTTTTTT CAAACTCCTG GAAGAATGTC AGGAATTGCT GGAGTATAAG 
AAGGCTGGAG CAGATGGCTT GATGGGAGCT ATGCCAGCGA ATGATCAAGG AGAAGGGCCC 
CTTCGATACT CCGAGAAGCT CTCACCTGCA GTCAATCAGT TTTTGATTGA GGCATCTCGG 
CAGGATGTCA GACTCACAAA GATTTCTCCA AATGCTCAGT GGATACTTCC ACAAACGAGT 
CCAGAGATTG GTTCATTCTG GAAAAGAGCA AGCCCTGTAA CAAATAGTTC AACTAGCCCA 
GGTCAGCCGC ATTCGTTGCG AGAGGCACTA GCCCAAGCAC AGTCTGCGGG AAGAAGTGGA 
GGCAGGTCTC TTCGTGAGAC CTTGCATCCT ATGCTCCGCC AAAAGTTGGA GCTATGGGAG 
GAGAACCTTA GTGCCTCTGC TATGCTCCAA ATTTTGGAGG TACTTGAGAA GTTTATAAGT 
GCAGTATCAA GTCAAGCTGT TCTCACTGAC TACATGAGGC TTGATTGTAT CACAACTATT 
TTTATTGGTT TTCTGGGACG CAGCCAGCCG TTGCCATTTT GGAAGGCCTT TCTACCTTTC 
TTCAATAACG TGTTTCGTCG TCATGGCGCT ACCTTGATGA GTAGGGAGAA TGATAGGTTC 
TTGAAGCAAG TAGCATTTCA TCTACTACGG CTCGGAGTTT TCCGGAATGA GTCCATACGG 
AAACGTTCTG TTGTTGGCCT TCAAATTCTT GTCCGAAATG CATACCACTA CTTTCAAGGC 
CTTTCAAGAC TGCGGGTGAT GCTTACAATC ACACTTTCTG AGCTCATGTC AGATGTTCAA 
GTAACACATC CTAAGATGGA TGGATCCTTG GAAGAGAGTG GTGAATCTCA ACGTCTTCGT 
AGGTCCTTGC AACAGATTGC ACAAGAAAGT ATTAGTGCGG ATCTTCTACG GGAATGTGGT 
CTTCCTGAAG ATTCTCTTAC TGCTTTGCCA GAAGGTGTGA ATGGCCAACA ATGGAGTTGG 
GCTGGAGTCG CTGAGCTTTC CACCTCTCTG CTGAAAGCTG TGGATGCTGC TGTAGGACAT 
GCTCTTTTGG GTCCTGAGAT TTTAATGAAT GACAAGTATG CAACTGCAGA GGCCTACCAT 
GGTTTGGCGA CGGCATATAC CAACGTGCCC GATCTGCATA TAATGTGGCT TCTGTATCTC 
TGCGAAGTTC ATCAAGGCAA CCAGTCTTAT GCAGAGGCTG CTCAGTGTGC TGTTGCGGTT 
GCAGGTGTCA TCATGCATGC TATAGTAGGA AAAGGTGATC ATGTTTGGGG AAAAGAGCAT 
GTTGAGGCAC TGGGTAATAT TTTCCCAATG CTCACTGGTT CCCGCTTTGG GGATGCTGCA 
ACTGCCGAAA TTGAAGGCTA TGGGTCATCG AAATTGACTG TCGAATCGGC AGTGAAGTAC 
TTGCAGCTCG CCAATAAACT CTTTGTCCAG GCAGAGCTCT TCCATTTCTG TGCCGGCATA 
TTGGAGCTCA TAATTCCCGT CCATAAGTCA AGGCGTGCCT ATAGTCAGCT GTCTAAATGT 
CATACTTCAT TGACCAGCAT CTACGAAGCT ATACTAGAGC AAGAGTCGAG CCCTATCCCT 
TTCACGGATG CAACATATTA TCGTGTGGGA TTTTATGGGA AAAGCTTTGG TAGTTTGCAC 
TGCAAGGAGT ATGTGTACAG GGAACCACGG GACGTGCGGT TAGGGGACAT TATGAAAAAT 
TTAGGAAATA TTTATGAGCC TACAGTAATT GAAGGAAAGC AGAACTTGCA TATTATCCCC 
GATTCTCGTC AAGTGAATCC AGAGGATCTT CAAGCTGGCA TTTGTTACTT GCAAATTACA 
TCTGTCGATC CTGTTACAGA GGACGAGGAT TTGGAAAGCA GGCGAGAAAG ACAATCCGGG 
AGGTCCTCGT CAACATTCAG TGCACGAGTC TTCGATCGAT TTTTATTTGA CACTCCTTTC 
ACAAAGAATG GAAGATCCCA AGGAGGTTTG GAAGATCAGT GGAAGAGGCG TACGGTTTTG 
TGGACTGAAG GGCCTTTTCC ATCTCTTGTA AATCGTTTAA TGGTTGTGAA GTCTGAGTCG 
CGAGAATTTT CGCCAATCGA AAACGCCATA GGAATGATTG AGACTCGTAC AGCAGCTTTA 
GCAGGAGAGC TCGATGATCA ACGTATAAAT GAGGGAGATC CTCTGCCCCG TTTACAAGGC 
CTGCAAAGAA TCTTGCAAGG AAGTGTTGCA GTCCAGGTAA ATAGTGGAGT GCTAGGTGTC 
TGCACAGCTT TCTTGTCTGG GGAACCTTCA ACACGATTGC GCTCACAAGA ACTACAGCAG 
CTGATAGCAG CACTCTTGGA GTTTATGGCT GTGTGCAAGA AAGCAATTCG AGTACATGCA 
CGACTGATTG GAGAGGAGGA CCAAGACTTC CACTCGCAGC TTGTGATTGG ATTCCAATCA 
CTCACAGCAG AGCTATCCCA CTATATTCCC GCCATCTTGT CAGATGTAAC CATACTTGGG 
AAACACAGCA ATGTGCTCAA GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC112279271 ( XM_024513632.1 ) cDNA ORF clone, -> XP_024369400.1 Physcomitrella patens guanine nucleotide exchange factor SPIKE 1-like (LOC112279271), transcript variant X12, mRNA.

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CloneID	OPn41285
Clone ID Related Accession (Same CDS sequence)	XM_024513632.1
Accession Version	XM_024513632.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Physcomitrella patens
Product	guanine nucleotide exchange factor SPIKE 1-like isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037253.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physcomitrella patens Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACGGTA CCTTGTGTCT CTATCATCGA GAGAAGCGGG AGAAATTATC AGAAAATTTT 
CACTTCCGAT GTTTACCATC AGAGTTCCAA GATTGTGTAA CTTGGCAGGA GGGTGGTAAT 
TGTCTGAGAA GAGCTATATT TTCACTGGAG GCAGCGTCGC CAGCAATATG TTTGTTGATC 
CAGCTTGAAA AGCATGTGAC TGAGGAAGGA GGAGTAACGG CTCATGTTTA TTCTCGAAAA 
GAACCTGTAC ACTTGACCGA GAGAGAAAAG CAGAAGTTAC AGGTCTGGGC GCGCGTGATG 
CCATTCAGGG AGCCTTTCGC GTGGGCAGTA GTTGCTCTGT TTGATGCAAA TATAACAGGA 
GGTGTAGGAG GATTCTCATC TTCTCCAAGC AGCCCACTTC CACCAGGACT TTTTGGACCA 
TCTTTGATGG AAGCATCCTT GGATTCTGAT GGACGACTTA TAGCTGATGC TAAGACAGAA 
TCATCTAGTG TGCCAGTTTT GGTTGATGTT CAGAGCCTTC GCCGGGTCAA AGAAAATTAC 
ACGGAGGAAA TGCTTCAGGA TCCCAAGTAC AAAGTGCACA AACCCGTGAA GGCCATCCTT 
CGTCTCGAGA TAGAGAGGTT ATCTACTGAA GATCATGACC AAGATTCCAT ATTTGAGTGT 
GGTAGTTTTG GTCGTGGGTC CACTGATGGT GATTCCCGCT TGACAGTAGG ACCCTCTTTC 
TCAGGGCGTT TCATGCAGGG GCTGGGCATG ACGAAGGTTA CGCATAATGG GCGGCAGAAA 
TGGAGCCCTG GAAGAAAACA TCGCTATCTG CATTCCGGCA GTGTTGGCAG CATTGAATTT 
GGAAAGTTTG ACTTCCGGGC TATTGAGTTT CGATCGGGTT CGAAAGTTGA GGTTTTACCT 
CAGCTATTGC ACTGCCTGTA TGTATATCCT CAATCAGTCA ATTTAAGTCG AAAACGCAAT 
CTTTTTGTGC GAGTGGAGCT TCGAAAGGAT GATGTAGACA TTCGCAAATC TCCTCTCGAG 
GCCCTTTATC CGAGGGATGC TGACAGCTCT ATGCAGAAAT ATGCACACTC TCAGATCGAT 
GCAAACACCA AGACACCTCA CTATCATGAT GAGTTCAAAA TTCAACTACC TGCCGTTCTC 
TCTCCTGAGC ATCACCTTCT GTTCACGTTT TTTCATGTGG ATCTACAGAT GAAAATTGAG 
GCTCCAAAGC CGGTGGTAGT GGGATACTCT GTTCTTCCGC TCATGTTTGT TTGTCAGGTT 
CAGCGGTTGG ACGGCAGCCT ACCTATCGCA AAGGAGCTGT ATTCCAATTA TCTACAAGAA 
AATGTGAAGG AAAGGTTGGA GTACTTGGAT GATGGCAAAA CTGTATTTAA ATTGAGGTCA 
CGATTATGCT CTTCTCTTTA TCCGGTTAAT GAGAGGATCC GAGATTTCTT TTCAGAATAC 
GATCGACATG TCTTGCGGAC CAGTCCACCC TGGGGTACTG AGCTGATGGA GGCTATTAAT 
GCTCTGAAGA CGGTTGAGCC GTCAGCTATG CTCCAGTTTC TTCAACCTAT TCTCAACATG 
CTGCTTCGTA TGATTCGCGA TGGAGGAGAA ACGTTGCAGG TTGCTGCATT CCGTTCAATG 
GTTAACATCA TTACCAGGGT ACAAGCAGAG ACATCTGATG GGGCTGAAAG AAATCGTTAC 
CTTGTGCATT ATGTGGATTA TGCATTTAAT GATTTTGGTG GGCGTCATGA GCCTGTGTAC 
CCAGGTCTGT GCAGTGTATG GCGCAGCCTT GCTCGAAGCA AGGCGAAAGG CTACCGTGTC 
GGACCTGTGT ATGATGATGT TCTTTCCATG GCTTGGGTGT TCCTGGAGCT AATTGTCAAG 
TCCATGGCAC TTGAACAATC CCGCATTTAC TCTGAGTCCC TCCCACCTGG AGAGGAGTTA 
CCACCTTTGC AGCTCATTGA TGAGGTCTTT AAAAGTATCG TACAGCTCTA TGATTGTTTA 
CTGACAGAGG TTCATGAGCG CTGCAAGAAG GGCATGCTTC TCGCAATGAA GCTGAATAGC 
AGTATTGCAT ACTTTTGCTA TGACCTTCTC TCTGTAATTG AGCATCGACA AGTCTTTGAG 
CTGGTTGCGC TTTATTTCAA CAAGTTTGCT GGGGTATGTC AATCTCTTGT GCACGAATGT 
AAGCTTAATT TTCTGCGCAT CGTCTGTGAC CATGATCTCT TTGTTGAGAT GCCCGGTCGA 
GATCCTACAG AGAGGAATTA TTTGGCATCA CTGTTGATGC AAGAGCTCTT TGTTACTTGG 
GATCATGAAG ATCTGACTTT AAGATCTAAG GCAGCTCGAA TCCTTGTGCA ACTTATGTGC 
AAGCATGAGT ATGATGCACG GTATCAAACA TTGGAGGACA AAATGTATAT TGCCCAACGT 
TACTTTCCAC TAGTTGACCT GATATTGGAC GAAATGCCAG TGTTCTATGG CCTGAGCACT 
ACAGAGAAGC GTGAAGTGCT GGTGTGTGTG CTTCACATTC TGCGATATCT AGACGATGCA 
TCTCTTGTTA AAGCTTGGCA GCACAACATG GATAGAACAA AGCTTTTTTT CAAACTCCTG 
GAAGAATGTC AGGAATTGCT GGAGTATAAG AAGGCTGGAG CAGATGGCTT GATGGGAGCT 
ATGCCAGCGA ATGATCAAGG AGAAGGGCCC CTTCGATACT CCGAGAAGCT CTCACCTGCA 
GTCAATCAGT TTTTGATTGA GGCATCTCGG CAGGATGTCA GACTCACAAA GATTTCTCCA 
AATGCTCAGT GGATACTTCC ACAAACGAGT CCAGAGATTG GTTCATTCTG GAAAAGAGCA 
AGCCCTGTAA CAAATAGTTC AACTAGCCCA GGTCAGCCGC ATTCGTTGCG AGAGGCACTA 
GCCCAAGCAC AGTCTGCGGG AAGAAGTGGA GGCAGGTCTC TTCGTGAGAC CTTGCATCCT 
ATGCTCCGCC AAAAGTTGGA GCTATGGGAG GAGAACCTTA GTGCCTCTGC TATGCTCCAA 
ATTTTGGAGG TACTTGAGAA GTTTATAAGT GCAGTATCAA GTCAAGCTGT TCTCACTGAC 
TACATGAGGC TTGATTGTAT CACAACTATT TTTATTGGTT TTCTGGGACG CAGCCAGCCG 
TTGCCATTTT GGAAGGCCTT TCTACCTTTC TTCAATAACG TGTTTCGTCG TCATGGCGCT 
ACCTTGATGA GTAGGGAGAA TGATAGGTTC TTGAAGCAAG TAGCATTTCA TCTACTACGG 
CTCGGAGTTT TCCGGAATGA GTCCATACGG AAACGTTCTG TTGTTGGCCT TCAAATTCTT 
GTCCGAAATG CATACCACTA CTTTCAAGGC CTTTCAAGAC TGCGGGTGAT GCTTACAATC 
ACACTTTCTG AGCTCATGTC AGATGTTCAA GTAACACATC CTAAGATGGA TGGATCCTTG 
GAAGAGAGTG GTGAATCTCA ACGTCTTCGT AGGTCCTTGC AACAGATTGC ACAAGAAAGT 
ATTAGTGCGG ATCTTCTACG GGAATGTGGT CTTCCTGAAG ATTCTCTTAC TGCTTTGCCA 
GAAGGTGTGA ATGGCCAACA ATGGAGTTGG GCTGGAGTCG CTGAGCTTTC CACCTCTCTG 
CTGAAAGCTG TGGATGCTGC TGTAGGACAT GCTCTTTTGG GTCCTGAGAT TTTAATGAAT 
GACAAGTATG CAACTGCAGA GGCCTACCAT GGTTTGGCGA CGGCATATAC CAACGTGCCC 
GATCTGCATA TAATGTGGCT TCTGTATCTC TGCGAAGTTC ATCAAGGCAA CCAGTCTTAT 
GCAGAGGCTG CTCAGTGTGC TGTTGCGGTT GCAGGTGTCA TCATGCATGC TATAGTAGGA 
AAAGGTGATC ATGTTTGGGG AAAAGAGCAT GTTGAGGCAC TGGGTAATAT TTTCCCAATG 
CTCACTGGTT CCCGCTTTGG GGATGCTGCA ACTGCCGAAA TTGAAGGCTA TGGGTCATCG 
AAATTGACTG TCGAATCGGC AGTGAAGTAC TTGCAGCTCG CCAATAAACT CTTTGTCCAG 
GCAGAGCTCT TCCATTTCTG TGCCGGCATA TTGGAGCTCA TAATTCCCGT CCATAAGTCA 
AGGCGTGCCT ATAGTCAGCT GTCTAAATGT CATACTTCAT TGACCAGCAT CTACGAAGCT 
ATACTAGAGC AAGAGTCGAG CCCTATCCCT TTCACGGATG CAACATATTA TCGTGTGGGA 
TTTTATGGGA AAAGCTTTGG TAGTTTGCAC TGCAAGGAGT ATGTGTACAG GGAACCACGG 
GACGTGCGGT TAGGGGACAT TATGAAAAAT TTAGGAAATA TTTATGAGCC TACAGTAATT 
GAAGGAAAGC AGAACTTGCA TATTATCCCC GATTCTCGTC AAGTGAATCC AGAGGATCTT 
CAAGCTGGCA TTTGTTACTT GCAAATTACA TCTGTCGATC CTGTTACAGA GGACGAGGAT 
TTGGAAAGCA GGCGAGAAAG ACAATCCGGG AGGTCCTCGT CAACATTCAG TGCACGAGTC 
TTCGATCGAT TTTTATTTGA CACTCCTTTC ACAAAGAATG GAAGATCCCA AGGAGGTTTG 
GAAGATCAGT GGAAGAGGCG TACGGTTTTG TGGACTGAAG GGCCTTTTCC ATCTCTTGTA 
AATCGTTTAA TGGTTGTGAA GTCTGAGTCG CGAGAATTTT CGCCAATCGA AAACGCCATA 
GGAATGATTG AGACTCGTAC AGCAGCTTTA GCAGGAGAGC TCGATGATCA ACGTATAAAT 
GAGGGAGATC CTCTGCCCCG TTTACAAGGC CTGCAAAGAA TCTTGCAAGG AAGTGTTGCA 
GTCCAGGTAA ATAGTGGAGT GCTAGGTGTC TGCACAGCTT TCTTGTCTGG GGAACCTTCA 
ACACGATTGC GCTCACAAGA ACTACAGCAG CTGATAGCAG CACTCTTGGA GTTTATGGCT 
GTGTGCAAGA AAGCAATTCG AGTACATGCA CGACTGATTG GAGAGGAGGA CCAAGACTTC 
CACTCGCAGC TTGTGATTGG ATTCCAATCA CTCACAGCAG AGCTATCCCA CTATATTCCC 
GCCATCTTGT CAGATGTAAC CATACTTGGG AAACACAGCA ATGTGCTCAA GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024369400.1
CDS	134..5230
Translation	MYGTLCLYHREKREKLSENFHFRCLPSEFQDCVTWQEGGNCLRRAIFSLEAASPAICLLIQLEKHVTEEGGVTAHVYSRKEPVHLTEREKQKLQVWARVMPFREPFAWAVVALFDANITGGVGGFSSSPSSPLPPGLFGPSLMEASLDSDGRLIADAKTESSSVPVLVDVQSLRRVKENYTEEMLQDPKYKVHKPVKAILRLEIERLSTEDHDQDSIFECGSFGRGSTDGDSRLTVGPSFSGRFMQGLGMTKVTHNGRQKWSPGRKHRYLHSGSVGSIEFGKFDFRAIEFRSGSKVEVLPQLLHCLYVYPQSVNLSRKRNLFVRVELRKDDVDIRKSPLEALYPRDADSSMQKYAHSQIDANTKTPHYHDEFKIQLPAVLSPEHHLLFTFFHVDLQMKIEAPKPVVVGYSVLPLMFVCQVQRLDGSLPIAKELYSNYLQENVKERLEYLDDGKTVFKLRSRLCSSLYPVNERIRDFFSEYDRHVLRTSPPWGTELMEAINALKTVEPSAMLQFLQPILNMLLRMIRDGGETLQVAAFRSMVNIITRVQAETSDGAERNRYLVHYVDYAFNDFGGRHEPVYPGLCSVWRSLARSKAKGYRVGPVYDDVLSMAWVFLELIVKSMALEQSRIYSESLPPGEELPPLQLIDEVFKSIVQLYDCLLTEVHERCKKGMLLAMKLNSSIAYFCYDLLSVIEHRQVFELVALYFNKFAGVCQSLVHECKLNFLRIVCDHDLFVEMPGRDPTERNYLASLLMQELFVTWDHEDLTLRSKAARILVQLMCKHEYDARYQTLEDKMYIAQRYFPLVDLILDEMPVFYGLSTTEKREVLVCVLHILRYLDDASLVKAWQHNMDRTKLFFKLLEECQELLEYKKAGADGLMGAMPANDQGEGPLRYSEKLSPAVNQFLIEASRQDVRLTKISPNAQWILPQTSPEIGSFWKRASPVTNSSTSPGQPHSLREALAQAQSAGRSGGRSLRETLHPMLRQKLELWEENLSASAMLQILEVLEKFISAVSSQAVLTDYMRLDCITTIFIGFLGRSQPLPFWKAFLPFFNNVFRRHGATLMSRENDRFLKQVAFHLLRLGVFRNESIRKRSVVGLQILVRNAYHYFQGLSRLRVMLTITLSELMSDVQVTHPKMDGSLEESGESQRLRRSLQQIAQESISADLLRECGLPEDSLTALPEGVNGQQWSWAGVAELSTSLLKAVDAAVGHALLGPEILMNDKYATAEAYHGLATAYTNVPDLHIMWLLYLCEVHQGNQSYAEAAQCAVAVAGVIMHAIVGKGDHVWGKEHVEALGNIFPMLTGSRFGDAATAEIEGYGSSKLTVESAVKYLQLANKLFVQAELFHFCAGILELIIPVHKSRRAYSQLSKCHTSLTSIYEAILEQESSPIPFTDATYYRVGFYGKSFGSLHCKEYVYREPRDVRLGDIMKNLGNIYEPTVIEGKQNLHIIPDSRQVNPEDLQAGICYLQITSVDPVTEDEDLESRRERQSGRSSSTFSARVFDRFLFDTPFTKNGRSQGGLEDQWKRRTVLWTEGPFPSLVNRLMVVKSESREFSPIENAIGMIETRTAALAGELDDQRINEGDPLPRLQGLQRILQGSVAVQVNSGVLGVCTAFLSGEPSTRLRSQELQQLIAALLEFMAVCKKAIRVHARLIGEEDQDFHSQLVIGFQSLTAELSHYIPAILSDVTILGKHSNVLKL

Target ORF information:

RefSeq Version	XM_024513632.1
Organism	Physcomitrella patens
Definition	Physcomitrella patens guanine nucleotide exchange factor SPIKE 1-like (LOC112279271), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024513632.1

ORF Insert Sequence:

ATGTACGGTA CCTTGTGTCT CTATCATCGA GAGAAGCGGG AGAAATTATC AGAAAATTTT 
CACTTCCGAT GTTTACCATC AGAGTTCCAA GATTGTGTAA CTTGGCAGGA GGGTGGTAAT 
TGTCTGAGAA GAGCTATATT TTCACTGGAG GCAGCGTCGC CAGCAATATG TTTGTTGATC 
CAGCTTGAAA AGCATGTGAC TGAGGAAGGA GGAGTAACGG CTCATGTTTA TTCTCGAAAA 
GAACCTGTAC ACTTGACCGA GAGAGAAAAG CAGAAGTTAC AGGTCTGGGC GCGCGTGATG 
CCATTCAGGG AGCCTTTCGC GTGGGCAGTA GTTGCTCTGT TTGATGCAAA TATAACAGGA 
GGTGTAGGAG GATTCTCATC TTCTCCAAGC AGCCCACTTC CACCAGGACT TTTTGGACCA 
TCTTTGATGG AAGCATCCTT GGATTCTGAT GGACGACTTA TAGCTGATGC TAAGACAGAA 
TCATCTAGTG TGCCAGTTTT GGTTGATGTT CAGAGCCTTC GCCGGGTCAA AGAAAATTAC 
ACGGAGGAAA TGCTTCAGGA TCCCAAGTAC AAAGTGCACA AACCCGTGAA GGCCATCCTT 
CGTCTCGAGA TAGAGAGGTT ATCTACTGAA GATCATGACC AAGATTCCAT ATTTGAGTGT 
GGTAGTTTTG GTCGTGGGTC CACTGATGGT GATTCCCGCT TGACAGTAGG ACCCTCTTTC 
TCAGGGCGTT TCATGCAGGG GCTGGGCATG ACGAAGGTTA CGCATAATGG GCGGCAGAAA 
TGGAGCCCTG GAAGAAAACA TCGCTATCTG CATTCCGGCA GTGTTGGCAG CATTGAATTT 
GGAAAGTTTG ACTTCCGGGC TATTGAGTTT CGATCGGGTT CGAAAGTTGA GGTTTTACCT 
CAGCTATTGC ACTGCCTGTA TGTATATCCT CAATCAGTCA ATTTAAGTCG AAAACGCAAT 
CTTTTTGTGC GAGTGGAGCT TCGAAAGGAT GATGTAGACA TTCGCAAATC TCCTCTCGAG 
GCCCTTTATC CGAGGGATGC TGACAGCTCT ATGCAGAAAT ATGCACACTC TCAGATCGAT 
GCAAACACCA AGACACCTCA CTATCATGAT GAGTTCAAAA TTCAACTACC TGCCGTTCTC 
TCTCCTGAGC ATCACCTTCT GTTCACGTTT TTTCATGTGG ATCTACAGAT GAAAATTGAG 
GCTCCAAAGC CGGTGGTAGT GGGATACTCT GTTCTTCCGC TCATGTTTGT TTGTCAGGTT 
CAGCGGTTGG ACGGCAGCCT ACCTATCGCA AAGGAGCTGT ATTCCAATTA TCTACAAGAA 
AATGTGAAGG AAAGGTTGGA GTACTTGGAT GATGGCAAAA CTGTATTTAA ATTGAGGTCA 
CGATTATGCT CTTCTCTTTA TCCGGTTAAT GAGAGGATCC GAGATTTCTT TTCAGAATAC 
GATCGACATG TCTTGCGGAC CAGTCCACCC TGGGGTACTG AGCTGATGGA GGCTATTAAT 
GCTCTGAAGA CGGTTGAGCC GTCAGCTATG CTCCAGTTTC TTCAACCTAT TCTCAACATG 
CTGCTTCGTA TGATTCGCGA TGGAGGAGAA ACGTTGCAGG TTGCTGCATT CCGTTCAATG 
GTTAACATCA TTACCAGGGT ACAAGCAGAG ACATCTGATG GGGCTGAAAG AAATCGTTAC 
CTTGTGCATT ATGTGGATTA TGCATTTAAT GATTTTGGTG GGCGTCATGA GCCTGTGTAC 
CCAGGTCTGT GCAGTGTATG GCGCAGCCTT GCTCGAAGCA AGGCGAAAGG CTACCGTGTC 
GGACCTGTGT ATGATGATGT TCTTTCCATG GCTTGGGTGT TCCTGGAGCT AATTGTCAAG 
TCCATGGCAC TTGAACAATC CCGCATTTAC TCTGAGTCCC TCCCACCTGG AGAGGAGTTA 
CCACCTTTGC AGCTCATTGA TGAGGTCTTT AAAAGTATCG TACAGCTCTA TGATTGTTTA 
CTGACAGAGG TTCATGAGCG CTGCAAGAAG GGCATGCTTC TCGCAATGAA GCTGAATAGC 
AGTATTGCAT ACTTTTGCTA TGACCTTCTC TCTGTAATTG AGCATCGACA AGTCTTTGAG 
CTGGTTGCGC TTTATTTCAA CAAGTTTGCT GGGGTATGTC AATCTCTTGT GCACGAATGT 
AAGCTTAATT TTCTGCGCAT CGTCTGTGAC CATGATCTCT TTGTTGAGAT GCCCGGTCGA 
GATCCTACAG AGAGGAATTA TTTGGCATCA CTGTTGATGC AAGAGCTCTT TGTTACTTGG 
GATCATGAAG ATCTGACTTT AAGATCTAAG GCAGCTCGAA TCCTTGTGCA ACTTATGTGC 
AAGCATGAGT ATGATGCACG GTATCAAACA TTGGAGGACA AAATGTATAT TGCCCAACGT 
TACTTTCCAC TAGTTGACCT GATATTGGAC GAAATGCCAG TGTTCTATGG CCTGAGCACT 
ACAGAGAAGC GTGAAGTGCT GGTGTGTGTG CTTCACATTC TGCGATATCT AGACGATGCA 
TCTCTTGTTA AAGCTTGGCA GCACAACATG GATAGAACAA AGCTTTTTTT CAAACTCCTG 
GAAGAATGTC AGGAATTGCT GGAGTATAAG AAGGCTGGAG CAGATGGCTT GATGGGAGCT 
ATGCCAGCGA ATGATCAAGG AGAAGGGCCC CTTCGATACT CCGAGAAGCT CTCACCTGCA 
GTCAATCAGT TTTTGATTGA GGCATCTCGG CAGGATGTCA GACTCACAAA GATTTCTCCA 
AATGCTCAGT GGATACTTCC ACAAACGAGT CCAGAGATTG GTTCATTCTG GAAAAGAGCA 
AGCCCTGTAA CAAATAGTTC AACTAGCCCA GGTCAGCCGC ATTCGTTGCG AGAGGCACTA 
GCCCAAGCAC AGTCTGCGGG AAGAAGTGGA GGCAGGTCTC TTCGTGAGAC CTTGCATCCT 
ATGCTCCGCC AAAAGTTGGA GCTATGGGAG GAGAACCTTA GTGCCTCTGC TATGCTCCAA 
ATTTTGGAGG TACTTGAGAA GTTTATAAGT GCAGTATCAA GTCAAGCTGT TCTCACTGAC 
TACATGAGGC TTGATTGTAT CACAACTATT TTTATTGGTT TTCTGGGACG CAGCCAGCCG 
TTGCCATTTT GGAAGGCCTT TCTACCTTTC TTCAATAACG TGTTTCGTCG TCATGGCGCT 
ACCTTGATGA GTAGGGAGAA TGATAGGTTC TTGAAGCAAG TAGCATTTCA TCTACTACGG 
CTCGGAGTTT TCCGGAATGA GTCCATACGG AAACGTTCTG TTGTTGGCCT TCAAATTCTT 
GTCCGAAATG CATACCACTA CTTTCAAGGC CTTTCAAGAC TGCGGGTGAT GCTTACAATC 
ACACTTTCTG AGCTCATGTC AGATGTTCAA GTAACACATC CTAAGATGGA TGGATCCTTG 
GAAGAGAGTG GTGAATCTCA ACGTCTTCGT AGGTCCTTGC AACAGATTGC ACAAGAAAGT 
ATTAGTGCGG ATCTTCTACG GGAATGTGGT CTTCCTGAAG ATTCTCTTAC TGCTTTGCCA 
GAAGGTGTGA ATGGCCAACA ATGGAGTTGG GCTGGAGTCG CTGAGCTTTC CACCTCTCTG 
CTGAAAGCTG TGGATGCTGC TGTAGGACAT GCTCTTTTGG GTCCTGAGAT TTTAATGAAT 
GACAAGTATG CAACTGCAGA GGCCTACCAT GGTTTGGCGA CGGCATATAC CAACGTGCCC 
GATCTGCATA TAATGTGGCT TCTGTATCTC TGCGAAGTTC ATCAAGGCAA CCAGTCTTAT 
GCAGAGGCTG CTCAGTGTGC TGTTGCGGTT GCAGGTGTCA TCATGCATGC TATAGTAGGA 
AAAGGTGATC ATGTTTGGGG AAAAGAGCAT GTTGAGGCAC TGGGTAATAT TTTCCCAATG 
CTCACTGGTT CCCGCTTTGG GGATGCTGCA ACTGCCGAAA TTGAAGGCTA TGGGTCATCG 
AAATTGACTG TCGAATCGGC AGTGAAGTAC TTGCAGCTCG CCAATAAACT CTTTGTCCAG 
GCAGAGCTCT TCCATTTCTG TGCCGGCATA TTGGAGCTCA TAATTCCCGT CCATAAGTCA 
AGGCGTGCCT ATAGTCAGCT GTCTAAATGT CATACTTCAT TGACCAGCAT CTACGAAGCT 
ATACTAGAGC AAGAGTCGAG CCCTATCCCT TTCACGGATG CAACATATTA TCGTGTGGGA 
TTTTATGGGA AAAGCTTTGG TAGTTTGCAC TGCAAGGAGT ATGTGTACAG GGAACCACGG 
GACGTGCGGT TAGGGGACAT TATGAAAAAT TTAGGAAATA TTTATGAGCC TACAGTAATT 
GAAGGAAAGC AGAACTTGCA TATTATCCCC GATTCTCGTC AAGTGAATCC AGAGGATCTT 
CAAGCTGGCA TTTGTTACTT GCAAATTACA TCTGTCGATC CTGTTACAGA GGACGAGGAT 
TTGGAAAGCA GGCGAGAAAG ACAATCCGGG AGGTCCTCGT CAACATTCAG TGCACGAGTC 
TTCGATCGAT TTTTATTTGA CACTCCTTTC ACAAAGAATG GAAGATCCCA AGGAGGTTTG 
GAAGATCAGT GGAAGAGGCG TACGGTTTTG TGGACTGAAG GGCCTTTTCC ATCTCTTGTA 
AATCGTTTAA TGGTTGTGAA GTCTGAGTCG CGAGAATTTT CGCCAATCGA AAACGCCATA 
GGAATGATTG AGACTCGTAC AGCAGCTTTA GCAGGAGAGC TCGATGATCA ACGTATAAAT 
GAGGGAGATC CTCTGCCCCG TTTACAAGGC CTGCAAAGAA TCTTGCAAGG AAGTGTTGCA 
GTCCAGGTAA ATAGTGGAGT GCTAGGTGTC TGCACAGCTT TCTTGTCTGG GGAACCTTCA 
ACACGATTGC GCTCACAAGA ACTACAGCAG CTGATAGCAG CACTCTTGGA GTTTATGGCT 
GTGTGCAAGA AAGCAATTCG AGTACATGCA CGACTGATTG GAGAGGAGGA CCAAGACTTC 
CACTCGCAGC TTGTGATTGG ATTCCAATCA CTCACAGCAG AGCTATCCCA CTATATTCCC 
GCCATCTTGT CAGATGTAAC CATACTTGGG AAACACAGCA ATGTGCTCAA GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC112279271 ( XM_024513635.1 ) cDNA ORF clone, -> XP_024369403.1 Physcomitrella patens guanine nucleotide exchange factor SPIKE 1-like (LOC112279271), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OPn41286
Clone ID Related Accession (Same CDS sequence)	XM_024513635.1
Accession Version	XM_024513635.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Physcomitrella patens
Product	guanine nucleotide exchange factor SPIKE 1-like isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037253.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physcomitrella patens Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACGGTA CCTTGTGTCT CTATCATCGA GAGAAGCGGG AGAAATTATC AGAAAATTTT 
CACTTCCGAT GTTTACCATC AGAGTTCCAA GATGAGGGTG GTAATTGTCT GAGAAGAGCT 
ATATTTTCAC TGGAGGCAGC GTCGCCAGCA ATATGTTTGT TGATCCAGCT TGAAAAGCAT 
GTGACTGAGG AAGGAGGAGT AACGGCTCAT GTTTATTCTC GAAAAGAACC TGTACACTTG 
ACCGAGAGAG AAAAGCAGAA GTTACAGGTC TGGGCGCGCG TGATGCCATT CAGGGAGCCT 
TTCGCGTGGG CAGTAGTTGC TCTGTTTGAT GCAAATATAA CAGGAGGTGT AGGAGGATTC 
TCATCTTCTC CAAGCAGCCC ACTTCCACCA GGACTTTTTG GACCATCTTT GATGGAAGCA 
TCCTTGGATT CTGATGGACG ACTTATAGCT GATGCTAAGA CAGAATCATC TAGTGTGCCA 
GTTTTGGTTG ATGTTCAGAG CCTTCGCCGG GTCAAAGAAA ATTACACGGA GGAAATGCTT 
CAGGATCCCA AGTACAAAGT GCACAAACCC GTGAAGGCCA TCCTTCGTCT CGAGATAGAG 
AGGTTATCTA CTGAAGATCA TGACCAAGAT TCCATATTTG AGTGTGGTAG TTTTGGTCGT 
GGGTCCACTG ATGGTGATTC CCGCTTGACA GTAGGACCCT CTTTCTCAGG GCGTTTCATG 
CAGGGGCTGG GCATGACGAA GGTTACGCAT AATGGGCGGC AGAAATGGAG CCCTGGAAGA 
AAACATCGCT ATCTGCATTC CGGCAGTGTT GGCAGCATTG AATTTGGAAA GTTTGACTTC 
CGGGCTATTG AGTTTCGATC GGGTTCGAAA GTTGAGGTTT TACCTCAGCT ATTGCACTGC 
CTGTATGTAT ATCCTCAATC AGTCAATTTA AGTCGAAAAC GCAATCTTTT TGTGCGAGTG 
GAGCTTCGAA AGGATGATGT AGACATTCGC AAATCTCCTC TCGAGGCCCT TTATCCGAGG 
GATGCTGACA GCTCTATGCA GAAATATGCA CACTCTCAGA TCGATGCAAA CACCAAGACA 
CCTCACTATC ATGATGAGTT CAAAATTCAA CTACCTGCCG TTCTCTCTCC TGAGCATCAC 
CTTCTGTTCA CGTTTTTTCA TGTGGATCTA CAGATGAAAA TTGAGGCTCC AAAGCCGGTG 
GTAGTGGGAT ACTCTGTTCT TCCGCTCATG TTTGTTTGTC AGGTTCAGCG GTTGGACGGC 
AGCCTACCTA TCGCAAAGGA GCTGTATTCC AATTATCTAC AAGAAAATGT GAAGGAAAGG 
TTGGAGTACT TGGATGATGG CAAAACTGTA TTTAAATTGA GGTCACGATT ATGCTCTTCT 
CTTTATCCGG TTAATGAGAG GATCCGAGAT TTCTTTTCAG AATACGATCG ACATGTCTTG 
CGGACCAGTC CACCCTGGGG TACTGAGCTG ATGGAGGCTA TTAATGCTCT GAAGACGGTT 
GAGCCGTCAG CTATGCTCCA GTTTCTTCAA CCTATTCTCA ACATGCTGCT TCGTATGATT 
CGCGATGGAG GAGAAACGTT GCAGGTTGCT GCATTCCGTT CAATGGTTAA CATCATTACC 
AGGGTACAAG CAGAGACATC TGATGGGGCT GAAAGAAATC GTTACCTTGT GCATTATGTG 
GATTATGCAT TTAATGATTT TGGTGGGCGT CATGAGCCTG TGTACCCAGG TCTGTGCAGT 
GTATGGCGCA GCCTTGCTCG AAGCAAGGCG AAAGGCTACC GTGTCGGACC TGTGTATGAT 
GATGTTCTTT CCATGGCTTG GGTGTTCCTG GAGCTAATTG TCAAGTCCAT GGCACTTGAA 
CAATCCCGCA TTTACTCTGA GTCCCTCCCA CCTGGAGAGG AGTTACCACC TTTGCAGCTC 
ATTGATGAGG TCTTTAAAAG TATCGTACAG CTCTATGATT GTTTACTGAC AGAGGTTCAT 
GAGCGCTGCA AGAAGGGCAT GCTTCTCGCA ATGAAGCTGA ATAGCAGTAT TGCATACTTT 
TGCTATGACC TTCTCTCTGT AATTGAGCAT CGACAAGTCT TTGAGCTGGT TGCGCTTTAT 
TTCAACAAGT TTGCTGGGGT ATGTCAATCT CTTGTGCACG AATGTAAGCT TAATTTTCTG 
CGCATCGTCT GTGACCATGA TCTCTTTGTT GAGATGCCCG GTCGAGATCC TACAGAGAGG 
AATTATTTGG CATCACTGTT GATGCAAGAG CTCTTTGTTA CTTGGGATCA TGAAGATCTG 
ACTTTAAGAT CTAAGGCAGC TCGAATCCTT GTGCAACTTA TGTGCAAGCA TGAGTATGAT 
GCACGGTATC AAACATTGGA GGACAAAATG TATATTGCCC AACGTTACTT TCCACTAGTT 
GACCTGATAT TGGACGAAAT GCCAGTGTTC TATGGCCTGA GCACTACAGA GAAGCGTGAA 
GTGCTGGTGT GTGTGCTTCA CATTCTGCGA TATCTAGACG ATGCATCTCT TGTTAAAGCT 
TGGCAGCACA ACATGGATAG AACAAAGCTT TTTTTCAAAC TCCTGGAAGA ATGTCAGGAA 
TTGCTGGAGT ATAAGAAGGC TGGAGCAGAT GGCTTGATGG GAGCTATGCC AGCGAATGAT 
CAAGGAGAAG GGCCCCTTCG ATACTCCGAG AAGCTCTCAC CTGCAGTCAA TCAGTTTTTG 
ATTGAGGCAT CTCGGCAGGA TGTCAGACTC ACAAAGATTT CTCCAAATGC TCAGTGGATA 
CTTCCACAAA CGAGTCCAGA GATTGGTTCA TTCTGGAAAA GAGCAAGCCC TGTAACAAAT 
AGTTCAACTA GCCCAGGTCA GCCGCATTCG TTGCGAGAGG CACTAGCCCA AGCACAGTCT 
GCGGGAAGAA GTGGAGGCAG GTCTCTTCGT GAGACCTTGC ATCCTATGCT CCGCCAAAAG 
TTGGAGCTAT GGGAGGAGAA CCTTAGTGCC TCTGCTATGC TCCAAATTTT GGAGGTACTT 
GAGAAGTTTA TAAGTGCAGT ATCAAGTCAA GCTGTTCTCA CTGACTACAT GAGGCTTGAT 
TGTATCACAA CTATTTTTAT TGGTTTTCTG GGACGCAGCC AGCCGTTGCC ATTTTGGAAG 
GCCTTTCTAC CTTTCTTCAA TAACGTGTTT CGTCGTCATG GCGCTACCTT GATGAGTAGG 
GAGAATGATA GGTTCTTGAA GCAAGTAGCA TTTCATCTAC TACGGCTCGG AGTTTTCCGG 
AATGAGTCCA TACGGAAACG TTCTGTTGTT GGCCTTCAAA TTCTTGTCCG AAATGCATAC 
CACTACTTTC AAGGCCTTTC AAGACTGCGG GTGATGCTTA CAATCACACT TTCTGAGCTC 
ATGTCAGATG TTCAAGTAAC ACATCCTAAG ATGGATGGAT CCTTGGAAGA GAGTGGTGAA 
TCTCAACGTC TTCGTAGGTC CTTGCAACAG ATTGCACAAG AAAGTATTAG TGCGGATCTT 
CTACGGGAAT GTGGTCTTCC TGAAGATTCT CTTACTGCTT TGCCAGAAGG TGTGAATGGC 
CAACAATGGA GTTGGGCTGG AGTCGCTGAG CTTTCCACCT CTCTGCTGAA AGCTGTGGAT 
GCTGCTGTAG GACATGCTCT TTTGGGTCCT GAGATTTTAA TGAATGACAA GTATGCAACT 
GCAGAGGCCT ACCATGGTTT GGCGACGGCA TATACCAACG TGCCCGATCT GCATATAATG 
TGGCTTCTGT ATCTCTGCGA AGTTCATCAA GGCAACCAGT CTTATGCAGA GGCTGCTCAG 
TGTGCTGTTG CGGTTGCAGG TGTCATCATG CATGCTATAG TAGGAAAAGG TGATCATGTT 
TGGGGAAAAG AGCATGTTGA GGCACTGGGT AATATTTTCC CAATGCTCAC TGGTTCCCGC 
TTTGGGGATG CTGCAACTGC CGAAATTGAA GGCTATGGGT CATCGAAATT GACTGTCGAA 
TCGGCAGTGA AGTACTTGCA GCTCGCCAAT AAACTCTTTG TCCAGGCAGA GCTCTTCCAT 
TTCTGTGCCG GCATATTGGA GCTCATAATT CCCGTCCATA AGTCAAGGCG TGCCTATAGT 
CAGCTGTCTA AATGTCATAC TTCATTGACC AGCATCTACG AAGCTATACT AGAGCAAGAG 
TCGAGCCCTA TCCCTTTCAC GGATGCAACA TATTATCGTG TGGGATTTTA TGGGAAAAGC 
TTTGGTAGTT TGCACTGCAA GGAGTATGTG TACAGGGAAC CACGGGACGT GCGGTTAGGG 
GACATTATGA AAAATTTAGG AAATATTTAT GAGCCTACAG TAATTGAAGG AAAGCAGAAC 
TTGCATATTA TCCCCGATTC TCGTCAAGTG AATCCAGAGG ATCTTCAAGC TGGCATTTGT 
TACTTGCAAA TTACATCTGT CGATCCTGTT ACAGAGGACG AGGATTTGGA AAGCAGGCGA 
GAAAGACAAT CCGGGAGGTC CTCGTCAACA TTCAGTGCAC GAGTCTTCGA TCGATTTTTA 
TTTGACACTC CTTTCACAAA GAATGGAAGA TCCCAAGGAG GTTTGGAAGA TCAGTGGAAG 
AGGCGTACGG TTTTGTGGAC TGAAGGGCCT TTTCCATCTC TTGTAAATCG TTTAATGGTT 
GTGAAGTCTG AGTCGCGAGA ATTTTCGCCA ATCGAAAACG CCATAGGAAT GATTGAGACT 
CGTACAGCAG CTTTAGCAGG AGAGCTCGAT GATCAACGTA TAAATGAGGG AGATCCTCTG 
CCCCGTTTAC AAGGCCTGCA AAGAATCTTG CAAGGAAGTG TTGCAGTCCA GGTAAATAGT 
GGAGTGCTAG GTGTCTGCAC AGCTTTCTTG TCTGGGGAAC CTTCAACACG ATTGCGCTCA 
CAAGAACTAC AGCAGCTGAT AGCAGCACTC TTGGAGTTTA TGGCTGTGTG CAAGAAAGCA 
ATTCGAGTAC ATGCACGACT GATTGGAGAG GAGGACCAAG ACTTCCACTC GCAGCTTGTG 
ATTGGATTCC AATCACTCAC AGCAGAGCTA TCCCACTATA TTCCCGCCAT CTTGTCAGAT 
GTAACCATAC TTGGGAAACA CAGCAATGTG CTCAAGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024369403.1
CDS	134..5215
Translation	MYGTLCLYHREKREKLSENFHFRCLPSEFQDEGGNCLRRAIFSLEAASPAICLLIQLEKHVTEEGGVTAHVYSRKEPVHLTEREKQKLQVWARVMPFREPFAWAVVALFDANITGGVGGFSSSPSSPLPPGLFGPSLMEASLDSDGRLIADAKTESSSVPVLVDVQSLRRVKENYTEEMLQDPKYKVHKPVKAILRLEIERLSTEDHDQDSIFECGSFGRGSTDGDSRLTVGPSFSGRFMQGLGMTKVTHNGRQKWSPGRKHRYLHSGSVGSIEFGKFDFRAIEFRSGSKVEVLPQLLHCLYVYPQSVNLSRKRNLFVRVELRKDDVDIRKSPLEALYPRDADSSMQKYAHSQIDANTKTPHYHDEFKIQLPAVLSPEHHLLFTFFHVDLQMKIEAPKPVVVGYSVLPLMFVCQVQRLDGSLPIAKELYSNYLQENVKERLEYLDDGKTVFKLRSRLCSSLYPVNERIRDFFSEYDRHVLRTSPPWGTELMEAINALKTVEPSAMLQFLQPILNMLLRMIRDGGETLQVAAFRSMVNIITRVQAETSDGAERNRYLVHYVDYAFNDFGGRHEPVYPGLCSVWRSLARSKAKGYRVGPVYDDVLSMAWVFLELIVKSMALEQSRIYSESLPPGEELPPLQLIDEVFKSIVQLYDCLLTEVHERCKKGMLLAMKLNSSIAYFCYDLLSVIEHRQVFELVALYFNKFAGVCQSLVHECKLNFLRIVCDHDLFVEMPGRDPTERNYLASLLMQELFVTWDHEDLTLRSKAARILVQLMCKHEYDARYQTLEDKMYIAQRYFPLVDLILDEMPVFYGLSTTEKREVLVCVLHILRYLDDASLVKAWQHNMDRTKLFFKLLEECQELLEYKKAGADGLMGAMPANDQGEGPLRYSEKLSPAVNQFLIEASRQDVRLTKISPNAQWILPQTSPEIGSFWKRASPVTNSSTSPGQPHSLREALAQAQSAGRSGGRSLRETLHPMLRQKLELWEENLSASAMLQILEVLEKFISAVSSQAVLTDYMRLDCITTIFIGFLGRSQPLPFWKAFLPFFNNVFRRHGATLMSRENDRFLKQVAFHLLRLGVFRNESIRKRSVVGLQILVRNAYHYFQGLSRLRVMLTITLSELMSDVQVTHPKMDGSLEESGESQRLRRSLQQIAQESISADLLRECGLPEDSLTALPEGVNGQQWSWAGVAELSTSLLKAVDAAVGHALLGPEILMNDKYATAEAYHGLATAYTNVPDLHIMWLLYLCEVHQGNQSYAEAAQCAVAVAGVIMHAIVGKGDHVWGKEHVEALGNIFPMLTGSRFGDAATAEIEGYGSSKLTVESAVKYLQLANKLFVQAELFHFCAGILELIIPVHKSRRAYSQLSKCHTSLTSIYEAILEQESSPIPFTDATYYRVGFYGKSFGSLHCKEYVYREPRDVRLGDIMKNLGNIYEPTVIEGKQNLHIIPDSRQVNPEDLQAGICYLQITSVDPVTEDEDLESRRERQSGRSSSTFSARVFDRFLFDTPFTKNGRSQGGLEDQWKRRTVLWTEGPFPSLVNRLMVVKSESREFSPIENAIGMIETRTAALAGELDDQRINEGDPLPRLQGLQRILQGSVAVQVNSGVLGVCTAFLSGEPSTRLRSQELQQLIAALLEFMAVCKKAIRVHARLIGEEDQDFHSQLVIGFQSLTAELSHYIPAILSDVTILGKHSNVLKL

Target ORF information:

RefSeq Version	XM_024513635.1
Organism	Physcomitrella patens
Definition	Physcomitrella patens guanine nucleotide exchange factor SPIKE 1-like (LOC112279271), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024513635.1

ORF Insert Sequence:

ATGTACGGTA CCTTGTGTCT CTATCATCGA GAGAAGCGGG AGAAATTATC AGAAAATTTT 
CACTTCCGAT GTTTACCATC AGAGTTCCAA GATGAGGGTG GTAATTGTCT GAGAAGAGCT 
ATATTTTCAC TGGAGGCAGC GTCGCCAGCA ATATGTTTGT TGATCCAGCT TGAAAAGCAT 
GTGACTGAGG AAGGAGGAGT AACGGCTCAT GTTTATTCTC GAAAAGAACC TGTACACTTG 
ACCGAGAGAG AAAAGCAGAA GTTACAGGTC TGGGCGCGCG TGATGCCATT CAGGGAGCCT 
TTCGCGTGGG CAGTAGTTGC TCTGTTTGAT GCAAATATAA CAGGAGGTGT AGGAGGATTC 
TCATCTTCTC CAAGCAGCCC ACTTCCACCA GGACTTTTTG GACCATCTTT GATGGAAGCA 
TCCTTGGATT CTGATGGACG ACTTATAGCT GATGCTAAGA CAGAATCATC TAGTGTGCCA 
GTTTTGGTTG ATGTTCAGAG CCTTCGCCGG GTCAAAGAAA ATTACACGGA GGAAATGCTT 
CAGGATCCCA AGTACAAAGT GCACAAACCC GTGAAGGCCA TCCTTCGTCT CGAGATAGAG 
AGGTTATCTA CTGAAGATCA TGACCAAGAT TCCATATTTG AGTGTGGTAG TTTTGGTCGT 
GGGTCCACTG ATGGTGATTC CCGCTTGACA GTAGGACCCT CTTTCTCAGG GCGTTTCATG 
CAGGGGCTGG GCATGACGAA GGTTACGCAT AATGGGCGGC AGAAATGGAG CCCTGGAAGA 
AAACATCGCT ATCTGCATTC CGGCAGTGTT GGCAGCATTG AATTTGGAAA GTTTGACTTC 
CGGGCTATTG AGTTTCGATC GGGTTCGAAA GTTGAGGTTT TACCTCAGCT ATTGCACTGC 
CTGTATGTAT ATCCTCAATC AGTCAATTTA AGTCGAAAAC GCAATCTTTT TGTGCGAGTG 
GAGCTTCGAA AGGATGATGT AGACATTCGC AAATCTCCTC TCGAGGCCCT TTATCCGAGG 
GATGCTGACA GCTCTATGCA GAAATATGCA CACTCTCAGA TCGATGCAAA CACCAAGACA 
CCTCACTATC ATGATGAGTT CAAAATTCAA CTACCTGCCG TTCTCTCTCC TGAGCATCAC 
CTTCTGTTCA CGTTTTTTCA TGTGGATCTA CAGATGAAAA TTGAGGCTCC AAAGCCGGTG 
GTAGTGGGAT ACTCTGTTCT TCCGCTCATG TTTGTTTGTC AGGTTCAGCG GTTGGACGGC 
AGCCTACCTA TCGCAAAGGA GCTGTATTCC AATTATCTAC AAGAAAATGT GAAGGAAAGG 
TTGGAGTACT TGGATGATGG CAAAACTGTA TTTAAATTGA GGTCACGATT ATGCTCTTCT 
CTTTATCCGG TTAATGAGAG GATCCGAGAT TTCTTTTCAG AATACGATCG ACATGTCTTG 
CGGACCAGTC CACCCTGGGG TACTGAGCTG ATGGAGGCTA TTAATGCTCT GAAGACGGTT 
GAGCCGTCAG CTATGCTCCA GTTTCTTCAA CCTATTCTCA ACATGCTGCT TCGTATGATT 
CGCGATGGAG GAGAAACGTT GCAGGTTGCT GCATTCCGTT CAATGGTTAA CATCATTACC 
AGGGTACAAG CAGAGACATC TGATGGGGCT GAAAGAAATC GTTACCTTGT GCATTATGTG 
GATTATGCAT TTAATGATTT TGGTGGGCGT CATGAGCCTG TGTACCCAGG TCTGTGCAGT 
GTATGGCGCA GCCTTGCTCG AAGCAAGGCG AAAGGCTACC GTGTCGGACC TGTGTATGAT 
GATGTTCTTT CCATGGCTTG GGTGTTCCTG GAGCTAATTG TCAAGTCCAT GGCACTTGAA 
CAATCCCGCA TTTACTCTGA GTCCCTCCCA CCTGGAGAGG AGTTACCACC TTTGCAGCTC 
ATTGATGAGG TCTTTAAAAG TATCGTACAG CTCTATGATT GTTTACTGAC AGAGGTTCAT 
GAGCGCTGCA AGAAGGGCAT GCTTCTCGCA ATGAAGCTGA ATAGCAGTAT TGCATACTTT 
TGCTATGACC TTCTCTCTGT AATTGAGCAT CGACAAGTCT TTGAGCTGGT TGCGCTTTAT 
TTCAACAAGT TTGCTGGGGT ATGTCAATCT CTTGTGCACG AATGTAAGCT TAATTTTCTG 
CGCATCGTCT GTGACCATGA TCTCTTTGTT GAGATGCCCG GTCGAGATCC TACAGAGAGG 
AATTATTTGG CATCACTGTT GATGCAAGAG CTCTTTGTTA CTTGGGATCA TGAAGATCTG 
ACTTTAAGAT CTAAGGCAGC TCGAATCCTT GTGCAACTTA TGTGCAAGCA TGAGTATGAT 
GCACGGTATC AAACATTGGA GGACAAAATG TATATTGCCC AACGTTACTT TCCACTAGTT 
GACCTGATAT TGGACGAAAT GCCAGTGTTC TATGGCCTGA GCACTACAGA GAAGCGTGAA 
GTGCTGGTGT GTGTGCTTCA CATTCTGCGA TATCTAGACG ATGCATCTCT TGTTAAAGCT 
TGGCAGCACA ACATGGATAG AACAAAGCTT TTTTTCAAAC TCCTGGAAGA ATGTCAGGAA 
TTGCTGGAGT ATAAGAAGGC TGGAGCAGAT GGCTTGATGG GAGCTATGCC AGCGAATGAT 
CAAGGAGAAG GGCCCCTTCG ATACTCCGAG AAGCTCTCAC CTGCAGTCAA TCAGTTTTTG 
ATTGAGGCAT CTCGGCAGGA TGTCAGACTC ACAAAGATTT CTCCAAATGC TCAGTGGATA 
CTTCCACAAA CGAGTCCAGA GATTGGTTCA TTCTGGAAAA GAGCAAGCCC TGTAACAAAT 
AGTTCAACTA GCCCAGGTCA GCCGCATTCG TTGCGAGAGG CACTAGCCCA AGCACAGTCT 
GCGGGAAGAA GTGGAGGCAG GTCTCTTCGT GAGACCTTGC ATCCTATGCT CCGCCAAAAG 
TTGGAGCTAT GGGAGGAGAA CCTTAGTGCC TCTGCTATGC TCCAAATTTT GGAGGTACTT 
GAGAAGTTTA TAAGTGCAGT ATCAAGTCAA GCTGTTCTCA CTGACTACAT GAGGCTTGAT 
TGTATCACAA CTATTTTTAT TGGTTTTCTG GGACGCAGCC AGCCGTTGCC ATTTTGGAAG 
GCCTTTCTAC CTTTCTTCAA TAACGTGTTT CGTCGTCATG GCGCTACCTT GATGAGTAGG 
GAGAATGATA GGTTCTTGAA GCAAGTAGCA TTTCATCTAC TACGGCTCGG AGTTTTCCGG 
AATGAGTCCA TACGGAAACG TTCTGTTGTT GGCCTTCAAA TTCTTGTCCG AAATGCATAC 
CACTACTTTC AAGGCCTTTC AAGACTGCGG GTGATGCTTA CAATCACACT TTCTGAGCTC 
ATGTCAGATG TTCAAGTAAC ACATCCTAAG ATGGATGGAT CCTTGGAAGA GAGTGGTGAA 
TCTCAACGTC TTCGTAGGTC CTTGCAACAG ATTGCACAAG AAAGTATTAG TGCGGATCTT 
CTACGGGAAT GTGGTCTTCC TGAAGATTCT CTTACTGCTT TGCCAGAAGG TGTGAATGGC 
CAACAATGGA GTTGGGCTGG AGTCGCTGAG CTTTCCACCT CTCTGCTGAA AGCTGTGGAT 
GCTGCTGTAG GACATGCTCT TTTGGGTCCT GAGATTTTAA TGAATGACAA GTATGCAACT 
GCAGAGGCCT ACCATGGTTT GGCGACGGCA TATACCAACG TGCCCGATCT GCATATAATG 
TGGCTTCTGT ATCTCTGCGA AGTTCATCAA GGCAACCAGT CTTATGCAGA GGCTGCTCAG 
TGTGCTGTTG CGGTTGCAGG TGTCATCATG CATGCTATAG TAGGAAAAGG TGATCATGTT 
TGGGGAAAAG AGCATGTTGA GGCACTGGGT AATATTTTCC CAATGCTCAC TGGTTCCCGC 
TTTGGGGATG CTGCAACTGC CGAAATTGAA GGCTATGGGT CATCGAAATT GACTGTCGAA 
TCGGCAGTGA AGTACTTGCA GCTCGCCAAT AAACTCTTTG TCCAGGCAGA GCTCTTCCAT 
TTCTGTGCCG GCATATTGGA GCTCATAATT CCCGTCCATA AGTCAAGGCG TGCCTATAGT 
CAGCTGTCTA AATGTCATAC TTCATTGACC AGCATCTACG AAGCTATACT AGAGCAAGAG 
TCGAGCCCTA TCCCTTTCAC GGATGCAACA TATTATCGTG TGGGATTTTA TGGGAAAAGC 
TTTGGTAGTT TGCACTGCAA GGAGTATGTG TACAGGGAAC CACGGGACGT GCGGTTAGGG 
GACATTATGA AAAATTTAGG AAATATTTAT GAGCCTACAG TAATTGAAGG AAAGCAGAAC 
TTGCATATTA TCCCCGATTC TCGTCAAGTG AATCCAGAGG ATCTTCAAGC TGGCATTTGT 
TACTTGCAAA TTACATCTGT CGATCCTGTT ACAGAGGACG AGGATTTGGA AAGCAGGCGA 
GAAAGACAAT CCGGGAGGTC CTCGTCAACA TTCAGTGCAC GAGTCTTCGA TCGATTTTTA 
TTTGACACTC CTTTCACAAA GAATGGAAGA TCCCAAGGAG GTTTGGAAGA TCAGTGGAAG 
AGGCGTACGG TTTTGTGGAC TGAAGGGCCT TTTCCATCTC TTGTAAATCG TTTAATGGTT 
GTGAAGTCTG AGTCGCGAGA ATTTTCGCCA ATCGAAAACG CCATAGGAAT GATTGAGACT 
CGTACAGCAG CTTTAGCAGG AGAGCTCGAT GATCAACGTA TAAATGAGGG AGATCCTCTG 
CCCCGTTTAC AAGGCCTGCA AAGAATCTTG CAAGGAAGTG TTGCAGTCCA GGTAAATAGT 
GGAGTGCTAG GTGTCTGCAC AGCTTTCTTG TCTGGGGAAC CTTCAACACG ATTGCGCTCA 
CAAGAACTAC AGCAGCTGAT AGCAGCACTC TTGGAGTTTA TGGCTGTGTG CAAGAAAGCA 
ATTCGAGTAC ATGCACGACT GATTGGAGAG GAGGACCAAG ACTTCCACTC GCAGCTTGTG 
ATTGGATTCC AATCACTCAC AGCAGAGCTA TCCCACTATA TTCCCGCCAT CTTGTCAGAT 
GTAACCATAC TTGGGAAACA CAGCAATGTG CTCAAGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Gene Symbol	LOC112279271
Entrez Gene ID	112279271

LOC112279271 cDNA ORF clone,

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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