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Dffa cDNA ORF clone, Rattus norvegicus(Norway rat)

Summary

Gene Symbol Dffa
Entrez Gene ID 114214
Full Name DNA fragmentation factor subunit alpha
Synonyms ICAD-S
General protein information
Preferred Names

DNA fragmentation factor subunit alpha

Names

DNA fragmentation factor subunit alpha
DNA fragmentation factor, alpha polypeptide
DNA fragmentation factor, alpha subunit
Gene Type protein-coding
Organism Rattus norvegicus(Norway rat)
Genome

5

5q36
Summary inhibitor of DFF40/CAD and caspase-dependent apoptotic DNA fragmentation [RGD, Feb 2006].

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_006239362.3 XP_006239424.1 DNA fragmentation factor subunit alpha isoform X1
NM_053679.2 NP_446131.2 DNA fragmentation factor subunit alpha

Pathways from BioSystems


Homologies


Rattus norvegicus (Norway rat) Dffa NP_446131.2
Gallus gallus (chicken) DFFA XP_417610.1
Bos taurus (cattle) DFFA NP_001068810.1
DFFA XP_001102195.1
Canis lupus familiaris (dog) DFFA XP_544574.2
Danio rerio (zebrafish) dffa NP_001002631.1
Homo sapiens (human) DFFA NP_004392.1
Pan troglodytes (chimpanzee) DFFA XP_001161941.1
Mus musculus (house mouse) Dffa NP_001020467.1
Xenopus tropicalis (tropical clawed frog) dffa XP_002939454.2

Gene Ontology


Bibliography

Related articles in PubMed

Apoptotic DNA degradation into oligonucleosomal fragments, but not apoptotic nuclear morphology, relies on a cytosolic pool of DFF40/CAD endonuclease.
Iglesias-Guimarais V, Gil-Gui?on E, Gabernet G, Garc?a-Belinch?n M, S?nchez-Osuna M, Casanelles E, Comella JX, Yuste VJ
The Journal of biological chemistry287(10)7766-79(2012 Mar)

Histone H1 subtype preferences of DFF40 and possible nuclear localization of DFF40/45 in normal and trichostatin A-treated NB4 leukemic cells.
Ninios YP, Sekeri-Pataryas KE, Sourlingas TG
Apoptosis : an international journal on programmed cell death15(2)128-38(2010 Feb)

Fifty C-terminal amino acid residues are necessary for the chaperone activity of DFF45 but not for the inhibition of DFF40.
Park HH
BMB reports42(11)713-8(2009 Nov)

Interaction of DNA fragmentation factor (DFF) with DNA reveals an unprecedented mechanism for nuclease inhibition and suggests that DFF can be activated in a DNA-bound state.
Korn C, Scholz SR, Gimadutdinow O, Lurz R, Pingoud A, Meiss G
The Journal of biological chemistry280(7)6005-15(2005 Feb)

Endonuclease G is required for early embryogenesis and normal apoptosis in mice.
Zhang J, Dong M, Li L, Fan Y, Pathre P, Dong J, Lou D, Wells JM, Olivares-Villag?mez D, Van Kaer L, Wang X, Xu M
Proceedings of the National Academy of Sciences of the United States of America100(26)15782-7(2003 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dffa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dffa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa10287 NM_053679.2
Latest version!		 Rattus norvegicus DNA fragmentation factor subunit alpha (Dffa), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $139.30
$199.00
ORa28878 XM_006239362.3
Latest version!		 Rattus norvegicus DNA fragmentation factor subunit alpha (Dffa), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID ORa10287
Clone ID Related Accession (Same CDS sequence) NM_053679.2
Accession Version NM_053679.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 798bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-27
Organism Rattus norvegicus(Norway rat)
Product DNA fragmentation factor subunit alpha
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC100067.1. On Aug 7, 2005 this sequence version replaced NM_053679.1. ##Evidence-Data-START## Transcript exon combination :: BC100067.1, AF136601.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGAGCTAT CCCGGGGAGC CAGCGCCCCG GACCCGGACG ATGTCGGGCC TCTCAAACCG 
TGTCTGCTTC GCCGCAACCA CAGCCGTGAG CAGCACGGCG TGGCAGCCTC CAGTCTCGAG 
GAGCTGAGGA GCAAAGCCTG TGAACTTCTG GCCATCGATA AGTCCCTGAC ACCAGTCACC 
CTGGTCCTGG CAGAGGATGG GACCATAGTG GACGATGAGG ACTATTTCCT CTGTCTCCCT 
TCCAACACAA AGTTTGTGGC ACTGGCCTGC AACGAGAAGT GGGCATACAA CGACTCGGAT 
GGAGGAACAG CTTGGCTTTC CCAAGAGTCC TTTGACACAG ATCAAACGGA CAGTGGGGCA 
GGGGTGAAGT GGAAGAATGT GGCCAGGCAG CTGAAAGAGG ATCTGTCCAG CATCATCCTG 
CTGTCAGAAG AGGAGCTCCA AGCACTCATC GACATCCCCT GTGCAGAGCT GGCGCAGGAA 
CTCTGCCAGA GTTGTGCCAC TGTCCAGGGG CTGCAGAGCA CGCTCCAGCA GGTGCTTGAC 
CAGAGAGAGG AAGCACGCCA GTCCAAGCAG CTCCTGGAAC TTTATCTCCA GGCCTTGGAG 
AAAGAGGGCA GCATCTTGTC CAACCAGAAC GAGTCCAAAG CTGCCCTTGG GGAAGAGCTG 
GATGCAGTTG ACACCGGCGT CGGCGGAGAG GTGGCTTCAG AAGTGATGCT GAGAAGCCAG 
ATCCTCGCAG CCCTGAAGGA GAAGCCGGCT CCAGAGCTGA GTCTGTCTAG TCAGGATTTA 
GAGGTGGGCA AGAACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_446131.2
CDS69..866
Translation

Target ORF information:

RefSeq Version NM_053679.2
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus DNA fragmentation factor subunit alpha (Dffa), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_053679.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGAGCTAT CCCGGGGAGC CAGCGCCCCG GACCCGGACG ATGTCGGGCC TCTCAAACCG 
TGTCTGCTTC GCCGCAACCA CAGCCGTGAG CAGCACGGCG TGGCAGCCTC CAGTCTCGAG 
GAGCTGAGGA GCAAAGCCTG TGAACTTCTG GCCATCGATA AGTCCCTGAC ACCAGTCACC 
CTGGTCCTGG CAGAGGATGG GACCATAGTG GACGATGAGG ACTATTTCCT CTGTCTCCCT 
TCCAACACAA AGTTTGTGGC ACTGGCCTGC AACGAGAAGT GGGCATACAA CGACTCGGAT 
GGAGGAACAG CTTGGCTTTC CCAAGAGTCC TTTGACACAG ATCAAACGGA CAGTGGGGCA 
GGGGTGAAGT GGAAGAATGT GGCCAGGCAG CTGAAAGAGG ATCTGTCCAG CATCATCCTG 
CTGTCAGAAG AGGAGCTCCA AGCACTCATC GACATCCCCT GTGCAGAGCT GGCGCAGGAA 
CTCTGCCAGA GTTGTGCCAC TGTCCAGGGG CTGCAGAGCA CGCTCCAGCA GGTGCTTGAC 
CAGAGAGAGG AAGCACGCCA GTCCAAGCAG CTCCTGGAAC TTTATCTCCA GGCCTTGGAG 
AAAGAGGGCA GCATCTTGTC CAACCAGAAC GAGTCCAAAG CTGCCCTTGG GGAAGAGCTG 
GATGCAGTTG ACACCGGCGT CGGCGGAGAG GTGGCTTCAG AAGTGATGCT GAGAAGCCAG 
ATCCTCGCAG CCCTGAAGGA GAAGCCGGCT CCAGAGCTGA GTCTGTCTAG TCAGGATTTA 
GAGGTGGGCA AGAACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa28878
Clone ID Related Accession (Same CDS sequence) XM_006239362.3
Accession Version XM_006239362.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 987bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product DNA fragmentation factor subunit alpha isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006239362.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGAGCTAT CCCGGGGAGC CAGCGCCCCG GACCCGGACG ATGTCGGGCC TCTCAAACCG 
TGTCTGCTTC GCCGCAACCA CAGCCGTGAG CAGCACGGCG TGGCAGCCTC CAGTCTCGAG 
GAGCTGAGGA GCAAAGCCTG TGAACTTCTG GCCATCGATA AGTCCCTGAC ACCAGTCACC 
CTGGTCCTGG CAGAGGATGG GACCATAGTG GACGATGAGG ACTATTTCCT CTGTCTCCCT 
TCCAACACAA AGTTTGTGGC ACTGGCCTGC AACGAGAAGT GGGCATACAA CGACTCGGAT 
GGAGGAACAG CTTGGCTTTC CCAAGAGTCC TTTGACACAG ATCAAACGGA CAGTGGGGCA 
GGGGTGAAGT GGAAGAATGT GGCCAGGCAG CTGAAAGAGG ATCTGTCCAG CATCATCCTG 
CTGTCAGAAG AGGAGCTCCA AGCACTCATC GACATCCCCT GTGCAGAGCT GGCGCAGGAA 
CTCTGCCAGA GTTGTGCCAC TGTCCAGGGG CTGCAGAGCA CGCTCCAGCA GGTGCTTGAC 
CAGAGAGAGG AAGCACGCCA GTCCAAGCAG CTCCTGGAAC TTTATCTCCA GGCCTTGGAG 
AAAGAGGGCA GCATCTTGTC CAACCAGAAC GAGTCCAAAG CTGCCCTTGG GGAAGAGCTG 
GATGCAGTTG ACACCGGCGT CGGCGGAGAG GTGGCTTCAG AAGTGATGCT GAGAAGCCAG 
ATCCTCGCAG CCCTGAAGGA GAAGCCGGCT CCAGAGCTGA GTCTGTCTAG TCAGGATTTA 
GAGTCGGTCT CCAAGGAGGA CCCCAAAGCC CTGGCTGTCG CTCTGAGCTG GGACATAAAG 
AAGGCAGAGA CAGTCCAGCA GGCCTGTACC ACGGAGCTCG CCCTGCGCCT GCAGCAAGTG 
CAGAGCTTGC ACTCACTCAG GAATCTTTCT GCAAAGAGGA AGCCACTGCC TGGGGACCCG 
CAGAGTCCCA AACGGGCCAA AGTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006239424.1
CDS99..1085
Translation

Target ORF information:

RefSeq Version XM_006239362.3
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus DNA fragmentation factor subunit alpha (Dffa), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006239362.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGAGCTAT CCCGGGGAGC CAGCGCCCCG GACCCGGACG ATGTCGGGCC TCTCAAACCG 
TGTCTGCTTC GCCGCAACCA CAGCCGTGAG CAGCACGGCG TGGCAGCCTC CAGTCTCGAG 
GAGCTGAGGA GCAAAGCCTG TGAACTTCTG GCCATCGATA AGTCCCTGAC ACCAGTCACC 
CTGGTCCTGG CAGAGGATGG GACCATAGTG GACGATGAGG ACTATTTCCT CTGTCTCCCT 
TCCAACACAA AGTTTGTGGC ACTGGCCTGC AACGAGAAGT GGGCATACAA CGACTCGGAT 
GGAGGAACAG CTTGGCTTTC CCAAGAGTCC TTTGACACAG ATCAAACGGA CAGTGGGGCA 
GGGGTGAAGT GGAAGAATGT GGCCAGGCAG CTGAAAGAGG ATCTGTCCAG CATCATCCTG 
CTGTCAGAAG AGGAGCTCCA AGCACTCATC GACATCCCCT GTGCAGAGCT GGCGCAGGAA 
CTCTGCCAGA GTTGTGCCAC TGTCCAGGGG CTGCAGAGCA CGCTCCAGCA GGTGCTTGAC 
CAGAGAGAGG AAGCACGCCA GTCCAAGCAG CTCCTGGAAC TTTATCTCCA GGCCTTGGAG 
AAAGAGGGCA GCATCTTGTC CAACCAGAAC GAGTCCAAAG CTGCCCTTGG GGAAGAGCTG 
GATGCAGTTG ACACCGGCGT CGGCGGAGAG GTGGCTTCAG AAGTGATGCT GAGAAGCCAG 
ATCCTCGCAG CCCTGAAGGA GAAGCCGGCT CCAGAGCTGA GTCTGTCTAG TCAGGATTTA 
GAGTCGGTCT CCAAGGAGGA CCCCAAAGCC CTGGCTGTCG CTCTGAGCTG GGACATAAAG 
AAGGCAGAGA CAGTCCAGCA GGCCTGTACC ACGGAGCTCG CCCTGCGCCT GCAGCAAGTG 
CAGAGCTTGC ACTCACTCAG GAATCTTTCT GCAAAGAGGA AGCCACTGCC TGGGGACCCG 
CAGAGTCCCA AACGGGCCAA AGTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Apoptotic DNA degradation into oligonucleosomal fragments, but not apoptotic nuclear morphology, relies on a cytosolic pool of DFF40/CAD endonuclease.
The Journal of biological chemistry287(10)7766-79(2012 Mar)
Iglesias-Guimarais V,Gil-Gui?on E,Gabernet G,Garc?a-Belinch?n M,S?nchez-Osuna M,Casanelles E,Comella JX,Yuste VJ


book

Histone H1 subtype preferences of DFF40 and possible nuclear localization of DFF40/45 in normal and trichostatin A-treated NB4 leukemic cells.
Apoptosis : an international journal on programmed cell death15(2)128-38(2010 Feb)
Ninios YP,Sekeri-Pataryas KE,Sourlingas TG


book

Fifty C-terminal amino acid residues are necessary for the chaperone activity of DFF45 but not for the inhibition of DFF40.
BMB reports42(11)713-8(2009 Nov)
Park HH


book

Interaction of DNA fragmentation factor (DFF) with DNA reveals an unprecedented mechanism for nuclease inhibition and suggests that DFF can be activated in a DNA-bound state.
The Journal of biological chemistry280(7)6005-15(2005 Feb)
Korn C,Scholz SR,Gimadutdinow O,Lurz R,Pingoud A,Meiss G


book

Endonuclease G is required for early embryogenesis and normal apoptosis in mice.
Proceedings of the National Academy of Sciences of the United States of America100(26)15782-7(2003 Dec)
Zhang J,Dong M,Li L,Fan Y,Pathre P,Dong J,Lou D,Wells JM,Olivares-Villag?mez D,Van Kaer L,Wang X,Xu M