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Alox5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Summary

Gene Symbol Alox5
Entrez Gene ID 25290
Full Name arachidonate 5-lipoxygenase
Synonyms LOX5A
General protein information
Preferred Names

arachidonate 5-lipoxygenase

Names

arachidonate 5-lipoxygenase
5 - Lipoxygenase
5-LO
5-lipoxygenase
Gene Type protein-coding
Organism Rattus norvegicus(Norway rat)
Genome

4

4q42
Summary catalyzes the conversion of arachidonate to leukotriene A4 in leukotriene metabolism [RGD, Feb 2006].

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_006237140.2 XP_006237202.1 arachidonate 5-lipoxygenase isoform X1
NM_012822.1 NP_036954.1 arachidonate 5-lipoxygenase
XM_017592481.1 XP_017447970.1 arachidonate 5-lipoxygenase isoform X2

Pathways from BioSystems


Homologies


Homo sapiens (human) ALOX5 NP_000689.1
Oryza sativa (rice) Os03g0738600 NP_001051212.1
Canis lupus familiaris (dog) ALOX5 NP_001192042.1
Bos taurus (cattle) ALOX5 NP_001179721.1
Arabidopsis thaliana (thale cress) LOX5 NP_188879.2
Pan troglodytes (chimpanzee) ALOX5 XP_507763.3
ALOX5 XP_001102354.1
Mus musculus (house mouse) Alox5 NP_033792.1
Rattus norvegicus (Norway rat) Alox5 NP_036954.1
Gallus gallus (chicken) ALOX5 XP_003641560.1
Xenopus tropicalis (tropical clawed frog) alox5 XP_004915982.1
Oryza sativa (rice) Os03g0700400 NP_001050993.1
Danio rerio (zebrafish) alox5a NP_001243676.1
Arabidopsis thaliana (thale cress) LOX1 NP_175900.1
Oryza sativa (rice) Os03g0699700 NP_001050990.1
Oryza sativa (rice) Os03g0700700 NP_001050995.2

Gene Ontology


Bibliography

Related articles in PubMed

Novel mechanism of regulation of the 5-lipoxygenase/leukotriene B
Tsuda S, Shinohara M, Oshita T, Nagao M, Tanaka N, Mori T, Hara T, Irino Y, Toh R, Ishida T, Hirata KI
Scientific reports7(1)12989(2017 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our results indicate that 5-lipoxygenase activation is mechanistically involved in chronic hepatic injury in a rat model of chronic aluminum overload exposure and that the 5-LO signaling pathway, which associated with inflammation and oxidative stress, is a potential therapeutic target for chronic non-infection liver diseases
Title: 5-lipoxygenase activation is involved in the mechanisms of chronic hepatic injury in a rat model of chronic aluminum overload exposure.

Results support 5-lipoxygenase involvement in the effect of glutoxim and molixan on intracellular Ca(2+) concentration in macrophages and indicate the inadvisability of a combined use of drugs glutoxim and molixan and antiasthmatic agent zileuton
Title: 5-Lipoxygenase inhibitor zileuton inhibits Ca(2+)-responses induced by glutoxim and molixan in macrophages.

12/15-LO-derived oxidized lipids regulate histone modifications associated with profibrotic gene expression in MCs, and 12/15-LO can mediate similar actions of TGF-beta1 and diabetes.
Title: Epigenetic Histone Modifications Involved in Profibrotic Gene Regulation by 12/15-Lipoxygenase and Its Oxidized Lipid Products in Diabetic Nephropathy.

Alox5 affects the osteogenic and adipogenic abilities of iPSCs in vivo and the effect of Cox2 inhibition in this system.
Title: Deletion of Alox5 gene decreases osteogenic differentiation but increases adipogenic differentiation of mouse induced pluripotent stem cells.

5-Lipoxygenase and 5-LO activating protein (FLAP) colocalize in the interstitium of the renal medulla following trauma and hemorrhagic shock..
Title: Mesenteric lymph diversion abrogates 5-lipoxygenase activation in the kidney following trauma and hemorrhagic shock.

The following Alox5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Alox5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa12975 NM_012822.1
Latest version!		 Rattus norvegicus arachidonate 5-lipoxygenase (Alox5), mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $391.30
$559.00
ORa27631 XM_006237140.2
Latest version!		 Rattus norvegicus arachidonate 5-lipoxygenase (Alox5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
ORa60769 XM_017592481.1
Latest version!		 Rattus norvegicus arachidonate 5-lipoxygenase (Alox5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID ORa12975
Clone ID Related Accession (Same CDS sequence) NM_012822.1
Accession Version NM_012822.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2013bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-24
Organism Rattus norvegicus(Norway rat)
Product arachidonate 5-lipoxygenase
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from J03960.1. On Jun 25, 2006 this sequence version replaced XM_001054699.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: J03960.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## 

1
61
121
181
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961
1021
1081
1141
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1261
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1501
1561
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1681
1741
1801
1861
1921
1981
ATGCCTTCCT ACACTGTCAC CGTAGCCACC GGTAGCCAGT GGTTCGCGGG CACCGACGAC 
TACATTTACC TCAGCCTCAT TGGCTCTGCA GGCTGCAGTG AGAAGCATCT TCTTGACAAA 
GCTTTCTACA ATGACTTCGA GCGCGGCGGT CGCGACTCCT ATGACGTCAC TGTGGATGAA 
GAACTGGGTG AGATCTACCT AGTCAAAATC GAGAAGCGCA AATACAGGCT CCATGATGAC 
TGGTACTTGA AATACATCAC ACTGAAGACA CCCCACGACT ACATAGAGTT CCCTTGTTAT 
CGTTGGATCA CAGGCGAGGG CGAGATTGTC CTGAGGGATG GATGTGCAAA ATTGGCCCGA 
GATGACCAAA TCCACATCCT CAAGCAGCAC AGGCGGAAAG AACTGGAAAC ACGTCAGAAA 
CAGTATCGAT GGATGGAGTG GAACCCCGGC TTCCCTTTGA GTATTGATGC TAAATGCCAC 
AAGGATCTGC CCCGAGATAT CCAGTTTGAT AGTGAAAAGG GAGTGGACTT TGTTCTGAAC 
TACTCAAAAG CGATGGAGAA CCTGTTCATC AATCGCTTCA TGCACATGTT CCAGTCTTCC 
TGGCATGACT TTGCTGACTT TGAGAAAATC TTCGTCAAAA TCAGCAACAC TATTTCTGAG 
AGAGTCAAGA ACCACTGGCA AGAGGACCTC ATGTTTGGCT ACCAGTTCCT GAATGGCTGC 
AACCCAGTAC TCATCAAGCG CTGCACAGAG TTGCCTAAGA AGCTCCCAGT GACCACAGAA 
ATGGTGGAGT GCAGCCTAGA GCGGCAGCTC AGTTTAGAAC AGGAAGTACA GGAAGGGAAC 
ATTTTCATCG TTGATTACGA ACTACTGGAT GGCATTGATG CTAACAAAAC TGACCCCTGT 
ACACACCAGT TCCTGGCCGC CCCCATCTGC CTGCTGTATA AGAACCTAGC CAACAAGATT 
GTTCCCATCG CCATCCAGCT CAACCAAACC CCTGGAGAGA AGAACCCAAT TTTCCTCCCT 
ACGGACTCAA AATACGACTG GCTTTTGGCC AAAATCTGGG TGCGTTCAAG TGACTTCCAT 
ATCCATCAAA CAATCACCCA TCTTCTCCGC ACACATCTGG TGTCTGAGGT GTTCGGTATT 
GCCATGTACC GCCAGCTGCC TGCTGTGCAC CCCCTTTTCA AGCTGCTGGT AGCCCATGTG 
AGGTTCACCA TTGCCATCAA CACTAAGGCC CGGGAACAGC TTAACTGTGA GTACGGCCTT 
TTTGACAAGG CCAATGCCAC CGGAGGTGGT GGGCACGTGC AGATGGTGCA GAGAGCTGTC 
CAGGACCTGA CCTATTCCTC CCTGTGCTTC CCGGAGGCCA TCAAGGCCCG GGGCATGGAC 
AACACCGAGG ACATCCCCTA CTACTTCTAT CGTGATGATG GACTGCTCGT GTGGGAAGCT 
ATCCAGTCGT TCACAACTGA GGTGGTAAGC ATCTACTATG AGGATGACCA GGTGGTGGAG 
GAGGACCAGG AACTGCAGGA CTTCGTGAAG GATGTTTACG TTTATGGCAT GCGGGGCAGA 
AAGGCCTCAG GTTTCCCCAA GTCCATCAAG AGCAGGGAGA AATTGTCTGA GTACCTGACG 
GTGGTGATCT TCACAGCCTC GGCCCAGCAT GCAGCTGTAA ACTTTGGCCA GTATGACTGG 
TGCTCCTGGA TCCCCAACGC TCCTCCAACT ATGCGGGCCC CACCACCCAC GGCCAAGGGT 
GTGGTCACCA TCGAGCAGAT TGTGGATACT CTACCAGACC GTGGCCGCTC ATGTTGGCAT 
CTAGGTGCAG TGTGGGCCTT GAGCCAGTTT CAAGAGAATG AGCTGTTTCT GGGCATGTAC 
CCAGAGGAGC ATTTCATCGA GAAGCCAGTG AAAGAAGCCA TGATTCGATT CCGCAAGAAC 
CTGGAGGCCA TCGTCAGCGT GATTGCCGAG CGCAATAAGA ACAAAAAGCT CCCCTACTAC 
TACCTGTCAC CAGACAGATT CCAAACAGTG TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_036954.1
CDS92..2104
Translation

Target ORF information:

RefSeq Version NM_012822.1
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus arachidonate 5-lipoxygenase (Alox5), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_012822.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGCCTTCCT ACACTGTCAC CGTAGCCACC GGTAGCCAGT GGTTCGCGGG CACCGACGAC 
TACATTTACC TCAGCCTCAT TGGCTCTGCA GGCTGCAGTG AGAAGCATCT TCTTGACAAA 
GCTTTCTACA ATGACTTCGA GCGCGGCGGT CGCGACTCCT ATGACGTCAC TGTGGATGAA 
GAACTGGGTG AGATCTACCT AGTCAAAATC GAGAAGCGCA AATACAGGCT CCATGATGAC 
TGGTACTTGA AATACATCAC ACTGAAGACA CCCCACGACT ACATAGAGTT CCCTTGTTAT 
CGTTGGATCA CAGGCGAGGG CGAGATTGTC CTGAGGGATG GATGTGCAAA ATTGGCCCGA 
GATGACCAAA TCCACATCCT CAAGCAGCAC AGGCGGAAAG AACTGGAAAC ACGTCAGAAA 
CAGTATCGAT GGATGGAGTG GAACCCCGGC TTCCCTTTGA GTATTGATGC TAAATGCCAC 
AAGGATCTGC CCCGAGATAT CCAGTTTGAT AGTGAAAAGG GAGTGGACTT TGTTCTGAAC 
TACTCAAAAG CGATGGAGAA CCTGTTCATC AATCGCTTCA TGCACATGTT CCAGTCTTCC 
TGGCATGACT TTGCTGACTT TGAGAAAATC TTCGTCAAAA TCAGCAACAC TATTTCTGAG 
AGAGTCAAGA ACCACTGGCA AGAGGACCTC ATGTTTGGCT ACCAGTTCCT GAATGGCTGC 
AACCCAGTAC TCATCAAGCG CTGCACAGAG TTGCCTAAGA AGCTCCCAGT GACCACAGAA 
ATGGTGGAGT GCAGCCTAGA GCGGCAGCTC AGTTTAGAAC AGGAAGTACA GGAAGGGAAC 
ATTTTCATCG TTGATTACGA ACTACTGGAT GGCATTGATG CTAACAAAAC TGACCCCTGT 
ACACACCAGT TCCTGGCCGC CCCCATCTGC CTGCTGTATA AGAACCTAGC CAACAAGATT 
GTTCCCATCG CCATCCAGCT CAACCAAACC CCTGGAGAGA AGAACCCAAT TTTCCTCCCT 
ACGGACTCAA AATACGACTG GCTTTTGGCC AAAATCTGGG TGCGTTCAAG TGACTTCCAT 
ATCCATCAAA CAATCACCCA TCTTCTCCGC ACACATCTGG TGTCTGAGGT GTTCGGTATT 
GCCATGTACC GCCAGCTGCC TGCTGTGCAC CCCCTTTTCA AGCTGCTGGT AGCCCATGTG 
AGGTTCACCA TTGCCATCAA CACTAAGGCC CGGGAACAGC TTAACTGTGA GTACGGCCTT 
TTTGACAAGG CCAATGCCAC CGGAGGTGGT GGGCACGTGC AGATGGTGCA GAGAGCTGTC 
CAGGACCTGA CCTATTCCTC CCTGTGCTTC CCGGAGGCCA TCAAGGCCCG GGGCATGGAC 
AACACCGAGG ACATCCCCTA CTACTTCTAT CGTGATGATG GACTGCTCGT GTGGGAAGCT 
ATCCAGTCGT TCACAACTGA GGTGGTAAGC ATCTACTATG AGGATGACCA GGTGGTGGAG 
GAGGACCAGG AACTGCAGGA CTTCGTGAAG GATGTTTACG TTTATGGCAT GCGGGGCAGA 
AAGGCCTCAG GTTTCCCCAA GTCCATCAAG AGCAGGGAGA AATTGTCTGA GTACCTGACG 
GTGGTGATCT TCACAGCCTC GGCCCAGCAT GCAGCTGTAA ACTTTGGCCA GTATGACTGG 
TGCTCCTGGA TCCCCAACGC TCCTCCAACT ATGCGGGCCC CACCACCCAC GGCCAAGGGT 
GTGGTCACCA TCGAGCAGAT TGTGGATACT CTACCAGACC GTGGCCGCTC ATGTTGGCAT 
CTAGGTGCAG TGTGGGCCTT GAGCCAGTTT CAAGAGAATG AGCTGTTTCT GGGCATGTAC 
CCAGAGGAGC ATTTCATCGA GAAGCCAGTG AAAGAAGCCA TGATTCGATT CCGCAAGAAC 
CTGGAGGCCA TCGTCAGCGT GATTGCCGAG CGCAATAAGA ACAAAAAGCT CCCCTACTAC 
TACCTGTCAC CAGACAGATT CCAAACAGTG TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa27631
Clone ID Related Accession (Same CDS sequence) XM_006237140.2
Accession Version XM_006237140.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2025bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product arachidonate 5-lipoxygenase isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006237140.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGCCTTCCT ACACTGTCAC CGTAGCCACC GGTAGCCAGT GGTTCGCGGG CACCGACGAC 
TACATTTACC TCAGCCTCAT TGGCTCTGCG GGCTGCAGTG AGAAGCATCT TCTTGACAAA 
GCTTTCTACA ATGACTTCGA GCGCGGCGCG GTGGACTCCT ATGACGTCAC TGTGGATGAA 
GAACTGGGTG AGATCTACCT AGTCAAAATC GAGAAGCGCA AATACTGGCT CCATGATGAC 
TGGTACTTGA AATACATCAC ACTGAAGACA CCCCACGGGG ACTACATAGA GTTCCCTTGT 
TATCGTTGGA TCACAGGCGA GGGCGAGATT GTCCTGAGGG ATGGATGTGC AAAATTGGCC 
CGAGATGACC AAATCCACAT CCTCAAGCAG CACAGGCGGA AAGAACTGGA AACACGTCAG 
AAACAGTATC GATGGATGGA GTGGAACCCC GGCTTCCCTT TGAGTATTGA TGCTAAATGC 
CACAAGGATC TGCCCCGAGA TATCCAGTTT GATAGTGAAA AGGGAGTGGA CTTTGTTCTG 
AACTACTCAA AAGCGATGGA GAACCTGTTC ATCAATCGCT TCATGCACAT GTTCCAGTCT 
TCCTGGCATG ACTTTGCTGA CTTTGAGAAA ATCTTCGTCA AAATCAGCAA CACTATTTCT 
GAGAGAGTCA AGAACCACTG GCAAGAGGAC CTCATGTTTG GCTACCAGTT CCTGAATGGC 
TGCAACCCAG TACTCATCAA GCGCTGCACA GAGTTGCCTA AGAAGCTCCC AGTGACCACA 
GAAATGGTGG AGTGCAGCCT AGAGCGGCAG CTCAGTTTAG AACAGGAAGT ACAGGAAGGG 
AACATTTTCA TCGTTGATTA CGAACTACTG GATGGCATTG ATGCTAACAA AACTGACCCC 
TGTACACACC AGTTCCTGGC CGCCCCCATC TGCCTGCTGT ATAAGAACCT AGCCAACAAG 
ATTGTTCCCA TCGCCATCCA GCTCAACCAA ACCCCTGGAG AGAAGAACCC AATTTTCCTC 
CCTACGGACT CAAAATACGA CTGGCTTTTG GCCAAAATCT GGGTGCGTTC AAGTGACTTC 
CATATCCATC AAACAATCAC CCATCTTCTC CGCACACATC TGGTGTCTGA GGTGTTCGGT 
ATTGCCATGT ACCGCCAGCT GCCTGCTGTG CACCCCCTTT TCAAGCTGCT GGTAGCCCAT 
GTGAGGTTCA CCATTGCCAT CAACACTAAG GCCCGGGAAC AGCTTATCTG TGAGTACGGC 
CTTTTTGACA AGGCCAATGC CACCGGAGGT GGTGGGCACG TGCAGATGGT GCAGAGAGCT 
GTCCAGGACC TGACCTATTC CTCCCTGTGC TTCCCGGAGG CCATCAAGGC CCGGGGCATG 
GACAACACCG AGGACATCCC CTACTACTTC TATCGTGATG ATGGACTGCT CGTGTGGGAA 
GCTATCCAGT CGTTCACAAC TGAGGTGGTA AGCATCTACT ATGAGGATGA CCAGGTGGTG 
GAGGAGGACC AGGAACTGCA GGACTTCGTG AAGGATGTTT ACGTTTATGG CATGCGGGGC 
AGAAAGGCCT CAGGTTTCCC CAAGTCCATC AAGAGCAGGG AGAAATTGTC TGAGTACCTG 
ACGGTGGTGA TCTTCACAGC CTCGGCCCAG CATGCAGCTG TAAACTTTGG CCAGTATGAC 
TGGTGCTCCT GGATCCCCAA CGCTCCTCCA ACTATGCGGG CCCCACCACC CACGGCCAAG 
GGTGTGGTCA CCATCGAGCA GATTGTGGAT ACTCTACCAG ACCGTGGCCG CTCATGTTGG 
CATCTAGGTG CAGTGTGGGC CTTGAGCCAG TTTCAAGAGA ATGAGCTGTT TCTGGGCATG 
TACCCAGAGG AGCATTTCAT CGAGAAGCCA GTGAAAGAAG CCATGATTCG ATTCCGCAAG 
AACCTGGAGG CCATCGTCAG CGTGATTGCC GAGCGCAATA AGAACAAAAA GCTCCCCTAC 
TACTACCTGT CACCAGACAG GATTCCAAAC AGTGTAGCCA TCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006237202.1
CDS126..2150
Translation

Target ORF information:

RefSeq Version XM_006237140.2
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus arachidonate 5-lipoxygenase (Alox5), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006237140.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGCCTTCCT ACACTGTCAC CGTAGCCACC GGTAGCCAGT GGTTCGCGGG CACCGACGAC 
TACATTTACC TCAGCCTCAT TGGCTCTGCG GGCTGCAGTG AGAAGCATCT TCTTGACAAA 
GCTTTCTACA ATGACTTCGA GCGCGGCGCG GTGGACTCCT ATGACGTCAC TGTGGATGAA 
GAACTGGGTG AGATCTACCT AGTCAAAATC GAGAAGCGCA AATACTGGCT CCATGATGAC 
TGGTACTTGA AATACATCAC ACTGAAGACA CCCCACGGGG ACTACATAGA GTTCCCTTGT 
TATCGTTGGA TCACAGGCGA GGGCGAGATT GTCCTGAGGG ATGGATGTGC AAAATTGGCC 
CGAGATGACC AAATCCACAT CCTCAAGCAG CACAGGCGGA AAGAACTGGA AACACGTCAG 
AAACAGTATC GATGGATGGA GTGGAACCCC GGCTTCCCTT TGAGTATTGA TGCTAAATGC 
CACAAGGATC TGCCCCGAGA TATCCAGTTT GATAGTGAAA AGGGAGTGGA CTTTGTTCTG 
AACTACTCAA AAGCGATGGA GAACCTGTTC ATCAATCGCT TCATGCACAT GTTCCAGTCT 
TCCTGGCATG ACTTTGCTGA CTTTGAGAAA ATCTTCGTCA AAATCAGCAA CACTATTTCT 
GAGAGAGTCA AGAACCACTG GCAAGAGGAC CTCATGTTTG GCTACCAGTT CCTGAATGGC 
TGCAACCCAG TACTCATCAA GCGCTGCACA GAGTTGCCTA AGAAGCTCCC AGTGACCACA 
GAAATGGTGG AGTGCAGCCT AGAGCGGCAG CTCAGTTTAG AACAGGAAGT ACAGGAAGGG 
AACATTTTCA TCGTTGATTA CGAACTACTG GATGGCATTG ATGCTAACAA AACTGACCCC 
TGTACACACC AGTTCCTGGC CGCCCCCATC TGCCTGCTGT ATAAGAACCT AGCCAACAAG 
ATTGTTCCCA TCGCCATCCA GCTCAACCAA ACCCCTGGAG AGAAGAACCC AATTTTCCTC 
CCTACGGACT CAAAATACGA CTGGCTTTTG GCCAAAATCT GGGTGCGTTC AAGTGACTTC 
CATATCCATC AAACAATCAC CCATCTTCTC CGCACACATC TGGTGTCTGA GGTGTTCGGT 
ATTGCCATGT ACCGCCAGCT GCCTGCTGTG CACCCCCTTT TCAAGCTGCT GGTAGCCCAT 
GTGAGGTTCA CCATTGCCAT CAACACTAAG GCCCGGGAAC AGCTTATCTG TGAGTACGGC 
CTTTTTGACA AGGCCAATGC CACCGGAGGT GGTGGGCACG TGCAGATGGT GCAGAGAGCT 
GTCCAGGACC TGACCTATTC CTCCCTGTGC TTCCCGGAGG CCATCAAGGC CCGGGGCATG 
GACAACACCG AGGACATCCC CTACTACTTC TATCGTGATG ATGGACTGCT CGTGTGGGAA 
GCTATCCAGT CGTTCACAAC TGAGGTGGTA AGCATCTACT ATGAGGATGA CCAGGTGGTG 
GAGGAGGACC AGGAACTGCA GGACTTCGTG AAGGATGTTT ACGTTTATGG CATGCGGGGC 
AGAAAGGCCT CAGGTTTCCC CAAGTCCATC AAGAGCAGGG AGAAATTGTC TGAGTACCTG 
ACGGTGGTGA TCTTCACAGC CTCGGCCCAG CATGCAGCTG TAAACTTTGG CCAGTATGAC 
TGGTGCTCCT GGATCCCCAA CGCTCCTCCA ACTATGCGGG CCCCACCACC CACGGCCAAG 
GGTGTGGTCA CCATCGAGCA GATTGTGGAT ACTCTACCAG ACCGTGGCCG CTCATGTTGG 
CATCTAGGTG CAGTGTGGGC CTTGAGCCAG TTTCAAGAGA ATGAGCTGTT TCTGGGCATG 
TACCCAGAGG AGCATTTCAT CGAGAAGCCA GTGAAAGAAG CCATGATTCG ATTCCGCAAG 
AACCTGGAGG CCATCGTCAG CGTGATTGCC GAGCGCAATA AGAACAAAAA GCTCCCCTAC 
TACTACCTGT CACCAGACAG GATTCCAAAC AGTGTAGCCA TCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa60769
Clone ID Related Accession (Same CDS sequence) XM_017592481.1
Accession Version XM_017592481.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1590bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product arachidonate 5-lipoxygenase isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGAGTGGA ACCCCGGCTT CCCTTTGAGT ATTGATGCTA AATGCCACAA GGATCTGCCC 
CGAGATATCC AGTTTGATAG TGAAAAGGGA GTGGACTTTG TTCTGAACTA CTCAAAAGCG 
ATGGAGAACC TGTTCATCAA TCGCTTCATG CACATGTTCC AGTCTTCCTG GCATGACTTT 
GCTGACTTTG AGAAAATCTT CGTCAAAATC AGCAACACTA TTTCTGAGAG AGTCAAGAAC 
CACTGGCAAG AGGACCTCAT GTTTGGCTAC CAGTTCCTGA ATGGCTGCAA CCCAGTACTC 
ATCAAGCGCT GCACAGAGTT GCCTAAGAAG CTCCCAGTGA CCACAGAAAT GGTGGAGTGC 
AGCCTAGAGC GGCAGCTCAG TTTAGAACAG GAAGTACAGG AAGGGAACAT TTTCATCGTT 
GATTACGAAC TACTGGATGG CATTGATGCT AACAAAACTG ACCCCTGTAC ACACCAGTTC 
CTGGCCGCCC CCATCTGCCT GCTGTATAAG AACCTAGCCA ACAAGATTGT TCCCATCGCC 
ATCCAGCTCA ACCAAACCCC TGGAGAGAAG AACCCAATTT TCCTCCCTAC GGACTCAAAA 
TACGACTGGC TTTTGGCCAA AATCTGGGTG CGTTCAAGTG ACTTCCATAT CCATCAAACA 
ATCACCCATC TTCTCCGCAC ACATCTGGTG TCTGAGGTGT TCGGTATTGC CATGTACCGC 
CAGCTGCCTG CTGTGCACCC CCTTTTCAAG CTGCTGGTAG CCCATGTGAG GTTCACCATT 
GCCATCAACA CTAAGGCCCG GGAACAGCTT ATCTGTGAGT ACGGCCTTTT TGACAAGGCC 
AATGCCACCG GAGGTGGTGG GCACGTGCAG ATGGTGCAGA GAGCTGTCCA GGACCTGACC 
TATTCCTCCC TGTGCTTCCC GGAGGCCATC AAGGCCCGGG GCATGGACAA CACCGAGGAC 
ATCCCCTACT ACTTCTATCG TGATGATGGA CTGCTCGTGT GGGAAGCTAT CCAGTCGTTC 
ACAACTGAGG TGGTAAGCAT CTACTATGAG GATGACCAGG TGGTGGAGGA GGACCAGGAA 
CTGCAGGACT TCGTGAAGGA TGTTTACGTT TATGGCATGC GGGGCAGAAA GGCCTCAGGT 
TTCCCCAAGT CCATCAAGAG CAGGGAGAAA TTGTCTGAGT ACCTGACGGT GGTGATCTTC 
ACAGCCTCGG CCCAGCATGC AGCTGTAAAC TTTGGCCAGT ATGACTGGTG CTCCTGGATC 
CCCAACGCTC CTCCAACTAT GCGGGCCCCA CCACCCACGG CCAAGGGTGT GGTCACCATC 
GAGCAGATTG TGGATACTCT ACCAGACCGT GGCCGCTCAT GTTGGCATCT AGGTGCAGTG 
TGGGCCTTGA GCCAGTTTCA AGAGAATGAG CTGTTTCTGG GCATGTACCC AGAGGAGCAT 
TTCATCGAGA AGCCAGTGAA AGAAGCCATG ATTCGATTCC GCAAGAACCT GGAGGCCATC 
GTCAGCGTGA TTGCCGAGCG CAATAAGAAC AAAAAGCTCC CCTACTACTA CCTGTCACCA 
GACAGGATTC CAAACAGTGT AGCCATCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_017447970.1
CDS460..2049
Translation

Target ORF information:

RefSeq Version XM_017592481.1
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus arachidonate 5-lipoxygenase (Alox5), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017592481.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGAGTGGA ACCCCGGCTT CCCTTTGAGT ATTGATGCTA AATGCCACAA GGATCTGCCC 
CGAGATATCC AGTTTGATAG TGAAAAGGGA GTGGACTTTG TTCTGAACTA CTCAAAAGCG 
ATGGAGAACC TGTTCATCAA TCGCTTCATG CACATGTTCC AGTCTTCCTG GCATGACTTT 
GCTGACTTTG AGAAAATCTT CGTCAAAATC AGCAACACTA TTTCTGAGAG AGTCAAGAAC 
CACTGGCAAG AGGACCTCAT GTTTGGCTAC CAGTTCCTGA ATGGCTGCAA CCCAGTACTC 
ATCAAGCGCT GCACAGAGTT GCCTAAGAAG CTCCCAGTGA CCACAGAAAT GGTGGAGTGC 
AGCCTAGAGC GGCAGCTCAG TTTAGAACAG GAAGTACAGG AAGGGAACAT TTTCATCGTT 
GATTACGAAC TACTGGATGG CATTGATGCT AACAAAACTG ACCCCTGTAC ACACCAGTTC 
CTGGCCGCCC CCATCTGCCT GCTGTATAAG AACCTAGCCA ACAAGATTGT TCCCATCGCC 
ATCCAGCTCA ACCAAACCCC TGGAGAGAAG AACCCAATTT TCCTCCCTAC GGACTCAAAA 
TACGACTGGC TTTTGGCCAA AATCTGGGTG CGTTCAAGTG ACTTCCATAT CCATCAAACA 
ATCACCCATC TTCTCCGCAC ACATCTGGTG TCTGAGGTGT TCGGTATTGC CATGTACCGC 
CAGCTGCCTG CTGTGCACCC CCTTTTCAAG CTGCTGGTAG CCCATGTGAG GTTCACCATT 
GCCATCAACA CTAAGGCCCG GGAACAGCTT ATCTGTGAGT ACGGCCTTTT TGACAAGGCC 
AATGCCACCG GAGGTGGTGG GCACGTGCAG ATGGTGCAGA GAGCTGTCCA GGACCTGACC 
TATTCCTCCC TGTGCTTCCC GGAGGCCATC AAGGCCCGGG GCATGGACAA CACCGAGGAC 
ATCCCCTACT ACTTCTATCG TGATGATGGA CTGCTCGTGT GGGAAGCTAT CCAGTCGTTC 
ACAACTGAGG TGGTAAGCAT CTACTATGAG GATGACCAGG TGGTGGAGGA GGACCAGGAA 
CTGCAGGACT TCGTGAAGGA TGTTTACGTT TATGGCATGC GGGGCAGAAA GGCCTCAGGT 
TTCCCCAAGT CCATCAAGAG CAGGGAGAAA TTGTCTGAGT ACCTGACGGT GGTGATCTTC 
ACAGCCTCGG CCCAGCATGC AGCTGTAAAC TTTGGCCAGT ATGACTGGTG CTCCTGGATC 
CCCAACGCTC CTCCAACTAT GCGGGCCCCA CCACCCACGG CCAAGGGTGT GGTCACCATC 
GAGCAGATTG TGGATACTCT ACCAGACCGT GGCCGCTCAT GTTGGCATCT AGGTGCAGTG 
TGGGCCTTGA GCCAGTTTCA AGAGAATGAG CTGTTTCTGG GCATGTACCC AGAGGAGCAT 
TTCATCGAGA AGCCAGTGAA AGAAGCCATG ATTCGATTCC GCAAGAACCT GGAGGCCATC 
GTCAGCGTGA TTGCCGAGCG CAATAAGAAC AAAAAGCTCC CCTACTACTA CCTGTCACCA 
GACAGGATTC CAAACAGTGT AGCCATCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Novel mechanism of regulation of the 5-lipoxygenase/leukotriene B
Scientific reports7(1)12989(2017 Oct)
Tsuda S,Shinohara M,Oshita T,Nagao M,Tanaka N,Mori T,Hara T,Irino Y,Toh R,Ishida T,Hirata KI