Pnlip cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pnlip
Entrez Gene ID	25702
Full Name	pancreatic lipase
Synonyms	PANLI
General protein information	Preferred Names pancreatic lipase Names pancreatic triacylglycerol lipase PL pancreatic triglyceride lipase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q55

mRNA and Protein(s)

mRNA	Protein	Name
NM_013161.2	NP_037293.1	pancreatic triacylglycerol lipase precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PNLIP	XP_001151144.1
Canis lupus familiaris (dog)	PNLIP	XP_535023.2
Bos taurus (cattle)	PNLIP	NP_001192749.1
Mus musculus (house mouse)	Pnlip	NP_081201.2
Drosophila melanogaster (fruit fly)	CG5966	NP_572286.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000211	XP_310922.4
	PNLIP	XP_001095070.1
Homo sapiens (human)	PNLIP	NP_000927.1
Rattus norvegicus (Norway rat)	Pnlip	NP_037293.1

Gene Ontology
Bibliography

Related articles in PubMed

A novel mutation in PNLIP causes pancreatic triglyceride lipase deficiency through protein misfolding.
Szab? A, Xiao X, Haughney M, Spector A, Sahin-T?th M, Lowe ME
Biochimica et biophysica acta1852(7)1372-9(2015 Jul)

Identification of a novel mutation in the PNLIP gene in two brothers with congenital pancreatic lipase deficiency.
Behar DM, Basel-Vanagaite L, Glaser F, Kaplan M, Tzur S, Magal N, Eidlitz-Markus T, Haimi-Cohen Y, Sarig G, Bormans C, Shohat M, Zeharia A
Journal of lipid research55(2)307-12(2014 Feb)

Mechanisms involved in the intestinal absorption of dietary vitamin A and provitamin A carotenoids.
Harrison EH
Biochimica et biophysica acta1821(1)70-7(2012 Jan)

Kinetic properties of mouse pancreatic lipase-related protein-2 suggest the mouse may not model human fat digestion.
Xiao X, Ross LE, Miller RA, Lowe ME
Journal of lipid research52(5)982-90(2011 May)

Pnlip encoding pancreatic lipase is a possible candidate for intramuscular fat accumulation QTL in OLETF rat.
Muramatsu Y, Yamada T, Taniguchi Y, Ogino T, Kose H, Matsumoto K, Sasaki Y
Research communications in molecular pathology and pharmacology120-121(1-6)23-31(2007-2008)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Regulated expression of pancreatic triglyceride lipase after rat traumatic brain injury
Title: Regulated expression of pancreatic triglyceride lipase after rat traumatic brain injury.

Pnlip encoding pancreatic lipase is a possible candidate for intramuscular fat accumulation QTL in OLETF rat.
Title: Pnlip encoding pancreatic lipase is a possible candidate for intramuscular fat accumulation QTL in OLETF rat.

Pnlip is possible candidate for the Nidd6/of QTL in the OLETF rat
Title: Pnlip encoding pancreatic lipase is possible candidate for obesity QTL in the OLETF rat.

Data show that pancreatic lipase and pancreatic lipase related protein 2 gene expression is regulated anticoordinately by the amount of dietary polyunsaturated fat starting as early as the preweanling phase of development.
Title: Pancreatic lipase and its related protein 2 are regulated by dietary polyunsaturated fat during the postnatal development of rats.

The following Pnlip gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pnlip cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02166	NM_013161.2 Latest version!	Rattus norvegicus pancreatic lipase (Pnlip), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$272.30 $389.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pnlip ( NM_013161.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037293.1 Rattus norvegicus pancreatic lipase (Pnlip), mRNA.

Price & Availability↑

CloneID	ORa02166
Clone ID Related Accession (Same CDS sequence)	NM_013161.2
Accession Version	NM_013161.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Rattus norvegicus(Norway rat)
Product	pancreatic triacylglycerol lipase precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M58369.1. On Aug 30, 2012 this sequence version replaced NM_013161.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M58369.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMN02642605 [ECO:0000348] ##Evidence-Data-END##

ATGCTGATGC TGTGGACATT TGCAGTGCTG CTGGGAGCAG TAGCTGGAAA AGAAGTTTGC 
TTTGACAAAC TCGGCTGCTT CAGTGATGAT GCCCCGTGGT CAGGAACCAT AGACAGACCC 
CTGAAAGCAC TGCCCTGGAG CCCAGCACAG ATCAACACCC GCTTTCTCCT CTACACCAAC 
GAGAACCAAG ACAACTACCA GAAAATTACT TCAGATGCAT CGAGTATCAG AAACTCCAAT 
TTCAAAACGA ACAGAAAAAC CCGCATCATT ATCCACGGCT TCATTGACAA GGGAGAGGAG 
AACTGGCTAT CTGACATGTG CAAGAACATG TTCAAAGTGG AGAGTGTGAA CTGCATCTGT 
GTGGACTGGA AAGGTGGCTC CCGGGCTACA TACACCCAGG CTACCCAGAA CGTCCGGGTC 
GTGGGTGCAG AAGTGGCATT ATTGGTTAAC GTTCTGAAGT CTGACCTCGG TCATCCACCT 
GACAACGTCC ACCTGATTGG CCACAGCCTG GGTTCCCACG TTGCTGGGGA GGCAGGAAAG 
AGGACATTCG GAGCTATTGG GAGGATCACA GGGTTGGACG CGGCTGAACC TTACTTCCAG 
GGCACACCTG AAGAAGTCCG TCTGGACCCC ACCGATGCCC AGTTTGTGGA TGCAATTCAC 
ACAGATGCTG CACCCATCAT CCCCAACTTA GGATTTGGAA TGAGCCAGAC TGTGGGTCAC 
CTAGATTTCT TTCCTAATGG AGGGATGGAA ATGCCTGGAT GTCAGAAGAA TATCCTTTCC 
CAAATCGTTG ACATCGACGG GATCTGGGAA GGAACTCGCG ACTTTGCTGC CTGTAACCAC 
TTGAGAAGCT ACAAATATTA CACCGACAGT ATCGTCAACC CGACTGGCTT CTCTGGCTTC 
TCCTGTAGCT CTTACAATGT TTTTTCTGCA AATAAGTGCT TCCCATGTGG GAGTGAAGGA 
TGCCCACAGA TGGGTCACTA TGCTGACAAA TACCCTGGGA AGACAAAAGA ACTCTACCAG 
AAATTTTACC TGAACACTGG TGACAAGAGC AACTTCGCAC GTTGGCGGTA CCAGGTGACT 
GTCACCCTCT CTGGACAGAA GGTCACTGGG CACATTCTAG TTTCTTTGTT TGGAAATGGA 
GGAAACTCTA AACAGTATGA AGTTTTCAAG GGCTCTCTGC ATCCAGGTGA CACTCATGTC 
AAGGAATTTG ACTCTGATAT GGATGTTGGA GATTTGCAGA AAGTTAAATT TATTTGGTAC 
AACAATGTGA TCAACCCAAC TCTACCCAAA GTGGGAGCAT CAAGGATTTC AGTGGAACGA 
AATGACGGCA GAGTGTTCAA CTTCTGTAGT CAAGATACAG TGAGGGAGGA TGTCCTGCTT 
ACACTGTCTG CATGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037293.1
CDS	29..1426
Translation	MLMLWTFAVLLGAVAGKEVCFDKLGCFSDDAPWSGTIDRPLKALPWSPAQINTRFLLYTNENQDNYQKITSDASSIRNSNFKTNRKTRIIIHGFIDKGEENWLSDMCKNMFKVESVNCICVDWKGGSRATYTQATQNVRVVGAEVALLVNVLKSDLGHPPDNVHLIGHSLGSHVAGEAGKRTFGAIGRITGLDAAEPYFQGTPEEVRLDPTDAQFVDAIHTDAAPIIPNLGFGMSQTVGHLDFFPNGGMEMPGCQKNILSQIVDIDGIWEGTRDFAACNHLRSYKYYTDSIVNPTGFSGFSCSSYNVFSANKCFPCGSEGCPQMGHYADKYPGKTKELYQKFYLNTGDKSNFARWRYQVTVTLSGQKVTGHILVSLFGNGGNSKQYEVFKGSLHPGDTHVKEFDSDMDVGDLQKVKFIWYNNVINPTLPKVGASRISVERNDGRVFNFCSQDTVREDVLLTLSAC

Target ORF information:

RefSeq Version	NM_013161.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus pancreatic lipase (Pnlip), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013161.2

ORF Insert Sequence:

ATGCTGATGC TGTGGACATT TGCAGTGCTG CTGGGAGCAG TAGCTGGAAA AGAAGTTTGC 
TTTGACAAAC TCGGCTGCTT CAGTGATGAT GCCCCGTGGT CAGGAACCAT AGACAGACCC 
CTGAAAGCAC TGCCCTGGAG CCCAGCACAG ATCAACACCC GCTTTCTCCT CTACACCAAC 
GAGAACCAAG ACAACTACCA GAAAATTACT TCAGATGCAT CGAGTATCAG AAACTCCAAT 
TTCAAAACGA ACAGAAAAAC CCGCATCATT ATCCACGGCT TCATTGACAA GGGAGAGGAG 
AACTGGCTAT CTGACATGTG CAAGAACATG TTCAAAGTGG AGAGTGTGAA CTGCATCTGT 
GTGGACTGGA AAGGTGGCTC CCGGGCTACA TACACCCAGG CTACCCAGAA CGTCCGGGTC 
GTGGGTGCAG AAGTGGCATT ATTGGTTAAC GTTCTGAAGT CTGACCTCGG TCATCCACCT 
GACAACGTCC ACCTGATTGG CCACAGCCTG GGTTCCCACG TTGCTGGGGA GGCAGGAAAG 
AGGACATTCG GAGCTATTGG GAGGATCACA GGGTTGGACG CGGCTGAACC TTACTTCCAG 
GGCACACCTG AAGAAGTCCG TCTGGACCCC ACCGATGCCC AGTTTGTGGA TGCAATTCAC 
ACAGATGCTG CACCCATCAT CCCCAACTTA GGATTTGGAA TGAGCCAGAC TGTGGGTCAC 
CTAGATTTCT TTCCTAATGG AGGGATGGAA ATGCCTGGAT GTCAGAAGAA TATCCTTTCC 
CAAATCGTTG ACATCGACGG GATCTGGGAA GGAACTCGCG ACTTTGCTGC CTGTAACCAC 
TTGAGAAGCT ACAAATATTA CACCGACAGT ATCGTCAACC CGACTGGCTT CTCTGGCTTC 
TCCTGTAGCT CTTACAATGT TTTTTCTGCA AATAAGTGCT TCCCATGTGG GAGTGAAGGA 
TGCCCACAGA TGGGTCACTA TGCTGACAAA TACCCTGGGA AGACAAAAGA ACTCTACCAG 
AAATTTTACC TGAACACTGG TGACAAGAGC AACTTCGCAC GTTGGCGGTA CCAGGTGACT 
GTCACCCTCT CTGGACAGAA GGTCACTGGG CACATTCTAG TTTCTTTGTT TGGAAATGGA 
GGAAACTCTA AACAGTATGA AGTTTTCAAG GGCTCTCTGC ATCCAGGTGA CACTCATGTC 
AAGGAATTTG ACTCTGATAT GGATGTTGGA GATTTGCAGA AAGTTAAATT TATTTGGTAC 
AACAATGTGA TCAACCCAAC TCTACCCAAA GTGGGAGCAT CAAGGATTTC AGTGGAACGA 
AATGACGGCA GAGTGTTCAA CTTCTGTAGT CAAGATACAG TGAGGGAGGA TGTCCTGCTT 
ACACTGTCTG CATGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A novel mutation in PNLIP causes pancreatic triglyceride lipase deficiency through protein misfolding.
Biochimica et biophysica acta1852(7)1372-9(2015 Jul)
Szab? A,Xiao X,Haughney M,Spector A,Sahin-T?th M,Lowe ME

Identification of a novel mutation in the PNLIP gene in two brothers with congenital pancreatic lipase deficiency.
Journal of lipid research55(2)307-12(2014 Feb)
Behar DM,Basel-Vanagaite L,Glaser F,Kaplan M,Tzur S,Magal N,Eidlitz-Markus T,Haimi-Cohen Y,Sarig G,Bormans C,Shohat M,Zeharia A

Mechanisms involved in the intestinal absorption of dietary vitamin A and provitamin A carotenoids.
Biochimica et biophysica acta1821(1)70-7(2012 Jan)
Harrison EH

Kinetic properties of mouse pancreatic lipase-related protein-2 suggest the mouse may not model human fat digestion.
Journal of lipid research52(5)982-90(2011 May)
Xiao X,Ross LE,Miller RA,Lowe ME

Pnlip encoding pancreatic lipase is a possible candidate for intramuscular fat accumulation QTL in OLETF rat.
Research communications in molecular pathology and pharmacology120-121(1-6)23-31(2007-2008)
Muramatsu Y,Yamada T,Taniguchi Y,Ogino T,Kose H,Matsumoto K,Sasaki Y