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Srd5a3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Summary

Gene Symbol Srd5a3
Entrez Gene ID 305291
Full Name steroid 5 alpha-reductase 3
Synonyms RGD1308828,S5AR 3
General protein information
Preferred Names

steroid 5 alpha-reductase 3

Names

polyprenol reductase
3-oxo-5-alpha-steroid 4-dehydrogenase 3
SR type 3
probable polyprenol reductase
Gene Type protein-coding
Organism Rattus norvegicus(Norway rat)
Genome

14

14p11

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001013990.1 NP_001014012.1 polyprenol reductase
XM_006250897.2 XP_006250959.1 polyprenol reductase isoform X1

Pathways from BioSystems


Homologies


Pan troglodytes (chimpanzee) SRD5A3 XP_526623.1
Canis lupus familiaris (dog) SRD5A3 XP_539274.2
Mus musculus (house mouse) Srd5a3 NP_065636.2
Gallus gallus (chicken) SRD5A3 XP_420703.2
Homo sapiens (human) SRD5A3 NP_078868.1
SRD5A3 XP_001088853.1
Rattus norvegicus (Norway rat) Srd5a3 NP_001014012.1
Caenorhabditis elegans (roundworm) B0024.13 NP_001256209.1
Bos taurus (cattle) SRD5A3 NP_001193019.1
Danio rerio (zebrafish) srd5a3 NP_001038404.1
Drosophila melanogaster (fruit fly) CG7840 NP_609203.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP007493 XP_308382.3
Xenopus tropicalis (tropical clawed frog) srd5a3 NP_001072539.1

Gene Ontology


Bibliography

Related articles in PubMed

SRD5A3 is required for converting polyprenol to dolichol and is mutated in a congenital glycosylation disorder.
Cantagrel V, Lefeber DJ, Ng BG, Guan Z, Silhavy JL, Bielas SL, Lehle L, Hombauer H, Adamowicz M, Swiezewska E, De Brouwer AP, Bl?mel P, Sykut-Cegielska J, Houliston S, Swistun D, Ali BR, Dobyns WB, Babovic-Vuksanovic D, van Bokhoven H, Wevers RA, Raetz CR, Freeze HH, Morava E, Al-Gazali L, Gleeson JG
Cell142(2)203-17(2010 Jul)

Novel 5 alpha-steroid reductase (SRD5A3, type-3) is overexpressed in hormone-refractory prostate cancer.
Uemura M, Tamura K, Chung S, Honma S, Okuyama A, Nakamura Y, Nakagawa H
Cancer science99(1)81-6(2008 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Srd5a3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Srd5a3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa04531 NM_001013990.1
Latest version!		 Rattus norvegicus steroid 5 alpha-reductase 3 (Srd5a3), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $188.30
$269.00
ORa36019 XM_006250897.2
Latest version!		 Rattus norvegicus steroid 5 alpha-reductase 3 (Srd5a3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID ORa04531
Clone ID Related Accession (Same CDS sequence) NM_001013990.1
Accession Version NM_001013990.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 837bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-10-01
Organism Rattus norvegicus(Norway rat)
Product polyprenol reductase
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC086584.1. On Apr 20, 2005 this sequence version replaced XM_223346.3. ##Evidence-Data-START## Transcript exon combination :: BC086584.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCTGGTT GGGCGGGGGC CGAGCTCTCG GTCCTGAACC CGCTGCGTGC GCTGTGGCTG 
TTGCTGGCCG CCGCCTTCCT GCTCGCACTG CTGCTGCAGC TGGCGCCCGC CAGGCTGCTA 
CCGAGCTGCG CGCTCTTCCA GGACCTCATC CGCTACGGGA AGACCAAGCA GTCCGGCTCG 
CGGCGCCCCG CCGTCTGCAG GGCCTTCGAC GTCCCCAAGA GGTACTTTTC TCACTTCTAC 
GTCGTCTCAG TGTTATGGAA TGGCTCCCTG CTCTGGTTCC TGTCTCAGTC TCTGTTCCTG 
GGAGCGCCGT TTCCAAGCTG GCTTTGGGCT TTGCTCAGAA CTCTTGGGGT CACGCAGTTC 
CAAGCCCTGG GGATGGAGTC CAAGGCTTCT CGGATACAAG CAGGCGAGCT GGCTCTGTCT 
ACCTTCTTAG TGTTGGTGTT CCTCTGGGTC CATAGTCTTC GGAGACTCTT CGAGTGCTTC 
TACGTCAGCG TCTTCTCTAA CACGGCCATT CACGTCGTGC AGTACTGTTT CGGGCTGGTC 
TACTATGTCC TTGTTGGCCT GACCGTACTG AGCCAAGTGC CCATGAATGA CAAGAACGTG 
TACGCTCTGG GGAAGAATCT ACTGCTACAA GCTCGGTGGT TCCACATCTT GGGAATGATG 
ATGTTCTTCT GGTCCTCTGC CCATCAGTAT AAGTGCCACG TCATTCTCAG CAATCTCAGG 
AGAAATAAGA AAGGTGTGGT CATCCACTGC CAGCACAGAA TCCCCTTTGG AGACTGGTTC 
GAGCTTTATG GTGAAGAGCG CAGCCCAGGT GACAGGTTCC CTTCCTCGAG ACGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001014012.1
CDS13..849
Misc Feature(1)61..123
Misc Feature(2)163..>798
Misc Feature(3)280..342
Misc Feature(4)421..483
Misc Feature(5)520..582
Misc Feature(6)631..693
Translation

Target ORF information:

RefSeq Version NM_001013990.1
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus steroid 5 alpha-reductase 3 (Srd5a3), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001013990.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCTGGTT GGGCGGGGGC CGAGCTCTCG GTCCTGAACC CGCTGCGTGC GCTGTGGCTG 
TTGCTGGCCG CCGCCTTCCT GCTCGCACTG CTGCTGCAGC TGGCGCCCGC CAGGCTGCTA 
CCGAGCTGCG CGCTCTTCCA GGACCTCATC CGCTACGGGA AGACCAAGCA GTCCGGCTCG 
CGGCGCCCCG CCGTCTGCAG GGCCTTCGAC GTCCCCAAGA GGTACTTTTC TCACTTCTAC 
GTCGTCTCAG TGTTATGGAA TGGCTCCCTG CTCTGGTTCC TGTCTCAGTC TCTGTTCCTG 
GGAGCGCCGT TTCCAAGCTG GCTTTGGGCT TTGCTCAGAA CTCTTGGGGT CACGCAGTTC 
CAAGCCCTGG GGATGGAGTC CAAGGCTTCT CGGATACAAG CAGGCGAGCT GGCTCTGTCT 
ACCTTCTTAG TGTTGGTGTT CCTCTGGGTC CATAGTCTTC GGAGACTCTT CGAGTGCTTC 
TACGTCAGCG TCTTCTCTAA CACGGCCATT CACGTCGTGC AGTACTGTTT CGGGCTGGTC 
TACTATGTCC TTGTTGGCCT GACCGTACTG AGCCAAGTGC CCATGAATGA CAAGAACGTG 
TACGCTCTGG GGAAGAATCT ACTGCTACAA GCTCGGTGGT TCCACATCTT GGGAATGATG 
ATGTTCTTCT GGTCCTCTGC CCATCAGTAT AAGTGCCACG TCATTCTCAG CAATCTCAGG 
AGAAATAAGA AAGGTGTGGT CATCCACTGC CAGCACAGAA TCCCCTTTGG AGACTGGTTC 
GAGCTTTATG GTGAAGAGCG CAGCCCAGGT GACAGGTTCC CTTCCTCGAG ACGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa36019
Clone ID Related Accession (Same CDS sequence) XM_006250897.2
Accession Version XM_006250897.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 993bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-26
Organism Rattus norvegicus(Norway rat)
Product polyprenol reductase isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006250897.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTGGTT GGGCGGGGGC CGAGCTCTCG GTCCTGAACC CGCTGCGTGC GCTGTGGCTG 
TTGCTGGCCG CCGCCTTCCT GCTCGCACTG CTGCTGCAGC TGGCGCCCGC CAGGCTGCTA 
CCGAGCTGCG CGCTCTTCCA GGACCTCATC CGCTACGGGA AGACCAAGCA GTCCGGCTCG 
CGGCGCCCCG CCGTCTGCAG GGCCTTCGAC GTCCCCAAGA GGTACTTTTC TCACTTCTAC 
GTCGTCTCAG TGTTATGGAA TGGCTCCCTG CTCTGGTTCC TGTCTCAGTC TCTGTTCCTG 
GGAGCGCCGT TTCCAAGCTG GCTTTGGGCT TTGCTCAGAA CTCTTGGGGT CACGCAGTTC 
CAAGCCCTGG GGATGGAGTC CAAGGCTTCT CGGATACAAG CAGGCGAGCT GGCTCTGTCT 
ACCTTCTTAG TGTTGGTGTT CCTCTGGGTC CATAGTCTTC GGAGACTCTT CGAGTGCTTC 
TACGTCAGCG TCTTCTCTAA CACGGCCATT CACGTCGTGC AGTACTGTTT CGGGCTGGTC 
TACTATGTCC TTGTTGGCCT GACCGTACTG AGCCAAGTGC CCATGAATGA CAAGAACGTG 
TACGCTCTGG GGAAGAATCT ACTGCTACAA GCTCGGTGGT TCCACATCTT GGGAATGATG 
ATGTTCTTCT GGTCCTCTGC CCATCAGTAT AAGTGCCACG TCATTCTCAG CAATCTCAGG 
AGAAATAAGA AAGGTGTGGT CATCCACTGC CAGCACAGAA TCCCCTTTGG AGACTGGTTC 
GAGTATGTGT CTTCTGCTAA CTACCTAGCA GAGCTGATGA TCTACATCTC CATGGCTGTC 
ACCTTCGGGC TCCACAACGT AACCTGGTGG CTGGTGGTGA CCTATGTCTT CTTCAGCCAA 
GCCTTGTCTG CGTTCTTCAA CCACAGGTTC TACAAAAGCA CATTTGTGTC CTACCCAAAG 
CATAGAAAAG CTTTCCTCCC GTTCTTGTTT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006250959.1
CDS68..1060
Misc Feature(1)218..1057
Translation

Target ORF information:

RefSeq Version XM_006250897.2
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus steroid 5 alpha-reductase 3 (Srd5a3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006250897.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTGGTT GGGCGGGGGC CGAGCTCTCG GTCCTGAACC CGCTGCGTGC GCTGTGGCTG 
TTGCTGGCCG CCGCCTTCCT GCTCGCACTG CTGCTGCAGC TGGCGCCCGC CAGGCTGCTA 
CCGAGCTGCG CGCTCTTCCA GGACCTCATC CGCTACGGGA AGACCAAGCA GTCCGGCTCG 
CGGCGCCCCG CCGTCTGCAG GGCCTTCGAC GTCCCCAAGA GGTACTTTTC TCACTTCTAC 
GTCGTCTCAG TGTTATGGAA TGGCTCCCTG CTCTGGTTCC TGTCTCAGTC TCTGTTCCTG 
GGAGCGCCGT TTCCAAGCTG GCTTTGGGCT TTGCTCAGAA CTCTTGGGGT CACGCAGTTC 
CAAGCCCTGG GGATGGAGTC CAAGGCTTCT CGGATACAAG CAGGCGAGCT GGCTCTGTCT 
ACCTTCTTAG TGTTGGTGTT CCTCTGGGTC CATAGTCTTC GGAGACTCTT CGAGTGCTTC 
TACGTCAGCG TCTTCTCTAA CACGGCCATT CACGTCGTGC AGTACTGTTT CGGGCTGGTC 
TACTATGTCC TTGTTGGCCT GACCGTACTG AGCCAAGTGC CCATGAATGA CAAGAACGTG 
TACGCTCTGG GGAAGAATCT ACTGCTACAA GCTCGGTGGT TCCACATCTT GGGAATGATG 
ATGTTCTTCT GGTCCTCTGC CCATCAGTAT AAGTGCCACG TCATTCTCAG CAATCTCAGG 
AGAAATAAGA AAGGTGTGGT CATCCACTGC CAGCACAGAA TCCCCTTTGG AGACTGGTTC 
GAGTATGTGT CTTCTGCTAA CTACCTAGCA GAGCTGATGA TCTACATCTC CATGGCTGTC 
ACCTTCGGGC TCCACAACGT AACCTGGTGG CTGGTGGTGA CCTATGTCTT CTTCAGCCAA 
GCCTTGTCTG CGTTCTTCAA CCACAGGTTC TACAAAAGCA CATTTGTGTC CTACCCAAAG 
CATAGAAAAG CTTTCCTCCC GTTCTTGTTT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

SRD5A3 is required for converting polyprenol to dolichol and is mutated in a congenital glycosylation disorder.
Cell142(2)203-17(2010 Jul)
Cantagrel V,Lefeber DJ,Ng BG,Guan Z,Silhavy JL,Bielas SL,Lehle L,Hombauer H,Adamowicz M,Swiezewska E,De Brouwer AP,Bl?mel P,Sykut-Cegielska J,Houliston S,Swistun D,Ali BR,Dobyns WB,Babovic-Vuksanovic D,van Bokhoven H,Wevers RA,Raetz CR,Freeze HH,Morava E,Al-Gazali L,Gleeson JG


book

Novel 5 alpha-steroid reductase (SRD5A3, type-3) is overexpressed in hormone-refractory prostate cancer.
Cancer science99(1)81-6(2008 Jan)
Uemura M,Tamura K,Chung S,Honma S,Okuyama A,Nakamura Y,Nakagawa H