Pip5k1c cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Gene Symbol	Pip5k1c
Entrez Gene ID	314641
Full Name	phosphatidylinositol-4-phosphate 5-kinase type 1 gamma
General protein information	Preferred Names phosphatidylinositol-4-phosphate 5-kinase type 1 gamma Names phosphatidylinositol 4-phosphate 5-kinase type-1 gamma PIP5K1-gamma PIP5KIgamma phosphatidylinositol 4-phosphate 5-kinase type I gamma ptdIns(4)P-5-kinase 1 gamma ptdIns(4)P-5-kinase gamma ptdInsPKIgamma
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q11

mRNA and Protein(s)

mRNA	Protein	Name
NM_001009967.2	NP_001009967.2	phosphatidylinositol 4-phosphate 5-kinase type-1 gamma isoform c
XM_008765103.2	XP_008763325.1	phosphatidylinositol 4-phosphate 5-kinase type-1 gamma isoform X2
XM_006240935.3	XP_006240997.1	phosphatidylinositol 4-phosphate 5-kinase type-1 gamma isoform X3
XM_017594818.1	XP_017450307.1	phosphatidylinositol 4-phosphate 5-kinase type-1 gamma isoform X4
XM_008765101.2	XP_008763323.1	phosphatidylinositol 4-phosphate 5-kinase type-1 gamma isoform X1
NM_001033970.1	NP_001029142.1	phosphatidylinositol 4-phosphate 5-kinase type-1 gamma isoform b

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PIP5K1C	XP_542172.5
Danio rerio (zebrafish)	si:ch211-243a15.1	XP_005171520.1
Homo sapiens (human)	PIP5K1C	NP_036530.1
Bos taurus (cattle)	PIP5K1C	NP_001192574.1
Danio rerio (zebrafish)	pip5k1cb	XP_005163702.1
Xenopus tropicalis (tropical clawed frog)	pip5k1c	NP_001120474.1
	LOC721744	XP_002801072.1
Rattus norvegicus (Norway rat)	Pip5k1c	NP_001009967.2
Pan troglodytes (chimpanzee)	PIP5K1C	XP_512274.3
Mus musculus (house mouse)	Pip5k1c	NP_032870.2
Gallus gallus (chicken)	PIP5K1C	XP_418191.4

Gene Ontology
Bibliography

Related articles in PubMed

LAPTM4B is a PtdIns(4,5)P2 effector that regulates EGFR signaling, lysosomal sorting, and degradation.
Tan X, Sun Y, Thapa N, Liao Y, Hedman AC, Anderson RA
The EMBO journal34(4)475-90(2015 Feb)

Platelets lacking PIP5KI? have normal integrin activation but impaired cytoskeletal-membrane integrity and adhesion.
Wang Y, Zhao L, Suzuki A, Lian L, Min SH, Wang Z, Litvinov RI, Stalker TJ, Yago T, Klopocki AG, Schmidtke DW, Yin H, Choi JK, McEver RP, Weisel JW, Hartwig JH, Abrams CS
Blood121(14)2743-52(2013 Apr)

Synaptic vesicle endocytosis.
Saheki Y, De Camilli P
Cold Spring Harbor perspectives in biology4(9)a005645(2012 Sep)

NMDA receptor-mediated PIP5K activation to produce PI(4,5)P? is essential for AMPA receptor endocytosis during LTD.
Unoki T, Matsuda S, Kakegawa W, Van NT, Kohda K, Suzuki A, Funakoshi Y, Hasegawa H, Yuzaki M, Kanaho Y
Neuron73(1)135-48(2012 Jan)

Phosphatidylinositol-4-phosphate 5-kinases and phosphatidylinositol 4,5-bisphosphate synthesis in the brain.
Volpicelli-Daley LA, Lucast L, Gong LW, Liu L, Sasaki J, Sasaki T, Abrams CS, Kanaho Y, De Camilli P
The Journal of biological chemistry285(37)28708-14(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pip5k1c gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pip5k1c cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa08798	NM_001033970.1 Latest version!	Rattus norvegicus phosphatidylinositol-4-phosphate 5-kinase type 1 gamma (Pip5k1c), transcript variant b, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa29870	XM_006240935.3 Latest version!	Rattus norvegicus phosphatidylinositol-4-phosphate 5-kinase type 1 gamma (Pip5k1c), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa01316	NM_001009967.2 Latest version!	Rattus norvegicus phosphatidylinositol-4-phosphate 5-kinase type 1 gamma (Pip5k1c), transcript variant c, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa47339	XM_008765101.2 Latest version!	Rattus norvegicus phosphatidylinositol-4-phosphate 5-kinase type 1 gamma (Pip5k1c), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa47341	XM_008765103.2 Latest version!	Rattus norvegicus phosphatidylinositol-4-phosphate 5-kinase type 1 gamma (Pip5k1c), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa61990	XM_017594818.1 Latest version!	Rattus norvegicus phosphatidylinositol-4-phosphate 5-kinase type 1 gamma (Pip5k1c), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pip5k1c ( NM_001033970.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001029142.1 Rattus norvegicus phosphatidylinositol-4-phosphate 5-kinase type 1 gamma (Pip5k1c), transcript variant b, mRNA.

Price & Availability↑

CloneID	ORa08798
Clone ID Related Accession (Same CDS sequence)	NM_001033970.1
Accession Version	NM_001033970.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	phosphatidylinositol 4-phosphate 5-kinase type-1 gamma isoform b
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CB557931.1, BC101909.1, CK366332.1, AY850259.1 and CB696331.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC101909.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCTAG AGGTGCCGGA CGAGGCAGAG AGCGCCGAAG CAGGGGCCGT GACAGCGGAG 
GCGGCCTGGT CTGCGGAGAG TGGGGCGGCG GCAGGTATGA CCCAGAAGAA GGCTATCCTT 
GCAGAGGCCC CGTTGGTGAC AGGGCAGCCA GGCCCTGGCC ATGGGAAGAA GCTGGGCCAT 
CGAGGCGTGG ATGCGTCTGG AGAGACTACG TATAAGAAGA CCACCTCGTC CACCCTGAAG 
GGTGCCATCC AACTGGGGAT TGGGTACACG GTGGGCAACC TCAGCTCTAA GCCAGAACGC 
GATGTGCTCA TGCAGGACTT CTATGTGGTG GAGAGCATCT TCTTTCCCAG CGAAGGGAGC 
AACCTTACCC CCGCCCACCA CTTCCAGGAT TTCCGTTTCA AGACCTATGC ACCTGTTGCC 
TTCCGTTACT TCCGGGAGCT CTTTGGTATC CGTCCGGACG ATTACTTGTA CTCACTCTGC 
AACGAGCCAC TCATTGAGCT GTCCAACCCG GGTGCCAGTG GCTCCGTCTT CTACGTCACC 
AGCGATGACG AGTTCATCAT TAAGACTGTC ATGCACAAGG AGGCGGAGTT CCTGCAGAAA 
CTGCTGCCTG GCTACTATAT GAACCTCAAC CAGAACCCCC GGACCTTGCT GCCCAAGTTC 
TATGGGCTGT ACTGTGTGCA GTCCGGCGGC AAGAACATTC GAGTGGTGGT CATGAACAAT 
GTGCTGCCCC GTGTCGTCAA GATGCACCTC AAGTTCGACC TCAAGGGCTC CACGTACAAG 
CGCAGGGCCA GCAAGAAGGA GAAGGAGAAG AGCCTGCCCA CCTACAAGGA CCTGGACTTC 
ATGCAGGACA TGCCAGAGGG GCTGCTACTG GACTCTGACA CCTTTGGCGC CCTGGTCAAG 
ACGCTGCAGC GAGACTGCCT GGTGTTGGAG AGCTTCAAGA TAATGGACTA CAGCCTGCTG 
CTGGGTGTGC ACAACATTGA CCAGCAGGAG CGGGAGCGCC AGGCCGAGGG CGCCCAGAGC 
AAGGCTGACG AGAAGCGGCC GGTGGCCCAG AAGGCCCTCT ACTCCACAGC CATGGAGTCC 
ATTCAGGGCG GAGCTGCCCG TGGGGAGGCC ATTGAGACAG ATGACACGAT GGGTGGGATC 
CCAGCCGTGA ATGGGCGTGG GGAGCGCTTG CTCCTACACA TCGGAATCAT TGACATTCTG 
CAGTCCTACA GGTTCATCAA GAAGTTAGAA CACACCTGGA AGGCCCTCGT CCACGATGGG 
GATACCGTCT CAGTCCACCG GCCCAGCTTC TATGCCGAGC GCTTCTTCAA GTTCATGAGC 
AGCACGGTCT TCCGGAAGAG TTCCTCCCTC AAGTCCTCTC CATCTAAGAA AGGGCGTGGC 
GCCCTGCTCG CAGTCAAGCC CCTGGGGCCC ACCGCAGCCT TCTCAGCTAG CCAGATCCCC 
AGTGAGAGAG AGGATGTGCA GTACGACCTG CGGGGGGCCC GCAGCTACCC CACGCTGGAG 
GATGAAGGCC GGCCTGACCT CCTGCCCTGC ACCCCACCGT CCTTTGAGGA AGCCACCACA 
GCCTCTATCG CCACCACCCT GTCATCCACC TCCCTCTCCA TCCCAGAGCG GTCCCCTTCA 
GACACATCAG AGCAGCCCCG GTACAGGCGG CGTACGCAGT CTTCAGGCCA GGATGGCCGG 
CCCCAGGAGG AGCTGCATGC GGAAGACCTG CAGAAGATAA CTGTGCAGGT GGAGCCAGTG 
TGTGGTGTGG GGGTTGTCGT CCCCAAGGAG CAGGGTGCAG GAGTGGAGGT TCCCCCATCT 
GGGGCATCGG CAGCAGCCAC GGTGGAAGTA GACGCTGCCA GCCAGGCCTC AGAGCCCGCC 
AGCCAAGCCT CTGATGAGGA AGATGCGCCT TCCACAGACA TCTATTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001029142.1
CDS	62..1972
Translation	MELEVPDEAESAEAGAVTAEAAWSAESGAAAGMTQKKAILAEAPLVTGQPGPGHGKKLGHRGVDASGETTYKKTTSSTLKGAIQLGIGYTVGNLSSKPERDVLMQDFYVVESIFFPSEGSNLTPAHHFQDFRFKTYAPVAFRYFRELFGIRPDDYLYSLCNEPLIELSNPGASGSVFYVTSDDEFIIKTVMHKEAEFLQKLLPGYYMNLNQNPRTLLPKFYGLYCVQSGGKNIRVVVMNNVLPRVVKMHLKFDLKGSTYKRRASKKEKEKSLPTYKDLDFMQDMPEGLLLDSDTFGALVKTLQRDCLVLESFKIMDYSLLLGVHNIDQQERERQAEGAQSKADEKRPVAQKALYSTAMESIQGGAARGEAIETDDTMGGIPAVNGRGERLLLHIGIIDILQSYRFIKKLEHTWKALVHDGDTVSVHRPSFYAERFFKFMSSTVFRKSSSLKSSPSKKGRGALLAVKPLGPTAAFSASQIPSEREDVQYDLRGARSYPTLEDEGRPDLLPCTPPSFEEATTASIATTLSSTSLSIPERSPSDTSEQPRYRRRTQSSGQDGRPQEELHAEDLQKITVQVEPVCGVGVVVPKEQGAGVEVPPSGASAAATVEVDAASQASEPASQASDEEDAPSTDIYF

Target ORF information:

RefSeq Version	NM_001033970.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphatidylinositol-4-phosphate 5-kinase type 1 gamma (Pip5k1c), transcript variant b, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001033970.1

ORF Insert Sequence:

ATGGAGCTAG AGGTGCCGGA CGAGGCAGAG AGCGCCGAAG CAGGGGCCGT GACAGCGGAG 
GCGGCCTGGT CTGCGGAGAG TGGGGCGGCG GCAGGTATGA CCCAGAAGAA GGCTATCCTT 
GCAGAGGCCC CGTTGGTGAC AGGGCAGCCA GGCCCTGGCC ATGGGAAGAA GCTGGGCCAT 
CGAGGCGTGG ATGCGTCTGG AGAGACTACG TATAAGAAGA CCACCTCGTC CACCCTGAAG 
GGTGCCATCC AACTGGGGAT TGGGTACACG GTGGGCAACC TCAGCTCTAA GCCAGAACGC 
GATGTGCTCA TGCAGGACTT CTATGTGGTG GAGAGCATCT TCTTTCCCAG CGAAGGGAGC 
AACCTTACCC CCGCCCACCA CTTCCAGGAT TTCCGTTTCA AGACCTATGC ACCTGTTGCC 
TTCCGTTACT TCCGGGAGCT CTTTGGTATC CGTCCGGACG ATTACTTGTA CTCACTCTGC 
AACGAGCCAC TCATTGAGCT GTCCAACCCG GGTGCCAGTG GCTCCGTCTT CTACGTCACC 
AGCGATGACG AGTTCATCAT TAAGACTGTC ATGCACAAGG AGGCGGAGTT CCTGCAGAAA 
CTGCTGCCTG GCTACTATAT GAACCTCAAC CAGAACCCCC GGACCTTGCT GCCCAAGTTC 
TATGGGCTGT ACTGTGTGCA GTCCGGCGGC AAGAACATTC GAGTGGTGGT CATGAACAAT 
GTGCTGCCCC GTGTCGTCAA GATGCACCTC AAGTTCGACC TCAAGGGCTC CACGTACAAG 
CGCAGGGCCA GCAAGAAGGA GAAGGAGAAG AGCCTGCCCA CCTACAAGGA CCTGGACTTC 
ATGCAGGACA TGCCAGAGGG GCTGCTACTG GACTCTGACA CCTTTGGCGC CCTGGTCAAG 
ACGCTGCAGC GAGACTGCCT GGTGTTGGAG AGCTTCAAGA TAATGGACTA CAGCCTGCTG 
CTGGGTGTGC ACAACATTGA CCAGCAGGAG CGGGAGCGCC AGGCCGAGGG CGCCCAGAGC 
AAGGCTGACG AGAAGCGGCC GGTGGCCCAG AAGGCCCTCT ACTCCACAGC CATGGAGTCC 
ATTCAGGGCG GAGCTGCCCG TGGGGAGGCC ATTGAGACAG ATGACACGAT GGGTGGGATC 
CCAGCCGTGA ATGGGCGTGG GGAGCGCTTG CTCCTACACA TCGGAATCAT TGACATTCTG 
CAGTCCTACA GGTTCATCAA GAAGTTAGAA CACACCTGGA AGGCCCTCGT CCACGATGGG 
GATACCGTCT CAGTCCACCG GCCCAGCTTC TATGCCGAGC GCTTCTTCAA GTTCATGAGC 
AGCACGGTCT TCCGGAAGAG TTCCTCCCTC AAGTCCTCTC CATCTAAGAA AGGGCGTGGC 
GCCCTGCTCG CAGTCAAGCC CCTGGGGCCC ACCGCAGCCT TCTCAGCTAG CCAGATCCCC 
AGTGAGAGAG AGGATGTGCA GTACGACCTG CGGGGGGCCC GCAGCTACCC CACGCTGGAG 
GATGAAGGCC GGCCTGACCT CCTGCCCTGC ACCCCACCGT CCTTTGAGGA AGCCACCACA 
GCCTCTATCG CCACCACCCT GTCATCCACC TCCCTCTCCA TCCCAGAGCG GTCCCCTTCA 
GACACATCAG AGCAGCCCCG GTACAGGCGG CGTACGCAGT CTTCAGGCCA GGATGGCCGG 
CCCCAGGAGG AGCTGCATGC GGAAGACCTG CAGAAGATAA CTGTGCAGGT GGAGCCAGTG 
TGTGGTGTGG GGGTTGTCGT CCCCAAGGAG CAGGGTGCAG GAGTGGAGGT TCCCCCATCT 
GGGGCATCGG CAGCAGCCAC GGTGGAAGTA GACGCTGCCA GCCAGGCCTC AGAGCCCGCC 
AGCCAAGCCT CTGATGAGGA AGATGCGCCT TCCACAGACA TCTATTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pip5k1c ( XM_006240935.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006240997.1 Rattus norvegicus phosphatidylinositol-4-phosphate 5-kinase type 1 gamma (Pip5k1c), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa29870
Clone ID Related Accession (Same CDS sequence)	XM_006240935.3
Accession Version	XM_006240935.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	phosphatidylinositol 4-phosphate 5-kinase type-1 gamma isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006240935.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTAG AGGTGCCGGA CGAGGCAGAG AGCGCCGAAG CAGGGGCCGT GACAGCGGAG 
GCGGCCTGGT CTGCGGAGAG TGGGGCGGCG GCAGGTATGA CCCAGAAGAA GGCTATCCTT 
GCAGAGGCCC CGTTGGTGAC AGGGCAGCCA GGCCCTGGCC ATGGGAAGAA GCTGGGCCAT 
CGAGGCGTGG ATGCGTCTGG AGAGACTACG TATAAGAAGA CCACCTCGTC CACCCTGAAG 
GGTGCCATCC AACTGGGGAT TGGGTACACG GTGGGCAACC TCAGCTCTAA GCCAGAACGC 
GATGTGCTCA TGCAGGACTT CTATGTGGTG GAGAGCATCT TCTTTCCCAG CGAAGGGAGC 
AACCTTACCC CCGCCCACCA CTTCCAGGAT TTCCGTTTCA AGACCTATGC ACCTGTTGCC 
TTCCGTTACT TCCGGGAGCT CTTTGGTATC CGTCCGGACG ATTACTTGTA CTCACTCTGC 
AACGAGCCAC TCATTGAGCT GTCCAACCCG GGTGCCAGTG GCTCCGTCTT CTACGTCACC 
AGCGATGACG AGTTCATCAT TAAGACTGTC ATGCACAAGG AGGCGGAGTT CCTGCAGAAA 
CTGCTGCCTG GCTACTATAT GAACCTCAAC CAGAACCCCC GGACCTTGCT GCCCAAGTTC 
TATGGGCTGT ACTGTGTGCA GTCCGGCGGC AAGAACATTC GAGTGGTGGT CATGAACAAT 
GTGCTGCCCC GTGTCGTCAA GATGCACCTC AAGTTCGACC TCAAGGGCTC CACGTACAAG 
CGCAGGGCCA GCAAGAAGGA GAAGGAGAAG AGCCTGCCCA CCTACAAGGA CCTGGACTTC 
ATGCAGGACA TGCCAGAGGG GCTGCTACTG GACTCTGACA CCTTTGGCGC CCTGGTCAAG 
ACGCTGCAGC GAGACTGCCT GGTGTTGGAG AGCTTCAAGA TAATGGACTA CAGCCTGCTG 
CTGGGTGTGC ACAACATTGA CCAGCAGGAG CGGGAGCGCC AGGCCGAGGG CGCCCAGAGC 
AAGGCTGACG AGAAGCGGCC GGTGGCCCAG AAGGCCCTCT ACTCCACAGC CATGGAGTCC 
ATTCAGGGCG GAGCTGCCCG TGGGGAGGCC ATTGAGACAG ATGACACGAT GGGTGGGATC 
CCAGCCGTGA ATGGGCGTGG GGAGCGCTTG CTCCTACACA TCGGAATCAT TGACATTCTG 
CAGTCCTACA GGTTCATCAA GAAGTTAGAA CACACCTGGA AGGCCCTCGT CCACGATGGG 
GATACCGTCT CAGTCCACCG GCCCAGCTTC TATGCCGAGC GCTTCTTCAA GTTCATGAGC 
AGCACGGTCT TCCGGAAGAG TTCCTCCCTC AAGTCCTCTC CATCTAAGAA AGGGCGTGGC 
GCCCTGCTCG CAGTCAAGCC CCTGGGGCCC ACCGCAGCCT TCTCAGCTAG CCAGATCCCC 
AGTGAGAGAG AGGATGTGCA GTACGACCTG CGGGGAGCCC GCAGCTACCC CACGCTGGAG 
GATGAAGGCC GGCCTGACCT CCTGCCCTGC ACCCCACCGT CCTTTGAGGA AGCCACCACA 
GCCTCTATCG CCACCACCCT GTCATCCACC TCCCTCTCCA TCCCAGAGCG GTCCCCTTCA 
GACACATCAG AGCAGCCCCG GTACAGGCGG CGTACGCAGT CTTCAGGCCA GGATGGCCGG 
CCCCAGGAGG AGCTGCATGC GGAAGACCTG CAGAAGATAA CTGTGCAGGT GGAGCCAGTG 
TGTGGTGTGG GGGTTGTCGT CCCCAAGGAG CAGGGTGCAG GAGTGGAGGT TCCCCCATCT 
GGGGCATCGG CAGCAGCCAC GGTGGAAGTA GACGCTGCCA GCCAGGCCTC AGAGCCCGCC 
AGCCAAGCCT CTGATGAGGA AGATGCGCCT TCCACAGACA TCTATTTTCC CACCGACGAG 
AGGAGCTGGG TGTACTCCCC GCTTCACTAT AGCGCACGGC CCGCCTCCGA CGGCGAGAGC 
GACACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006240997.1
CDS	103..2091
Translation	MELEVPDEAESAEAGAVTAEAAWSAESGAAAGMTQKKAILAEAPLVTGQPGPGHGKKLGHRGVDASGETTYKKTTSSTLKGAIQLGIGYTVGNLSSKPERDVLMQDFYVVESIFFPSEGSNLTPAHHFQDFRFKTYAPVAFRYFRELFGIRPDDYLYSLCNEPLIELSNPGASGSVFYVTSDDEFIIKTVMHKEAEFLQKLLPGYYMNLNQNPRTLLPKFYGLYCVQSGGKNIRVVVMNNVLPRVVKMHLKFDLKGSTYKRRASKKEKEKSLPTYKDLDFMQDMPEGLLLDSDTFGALVKTLQRDCLVLESFKIMDYSLLLGVHNIDQQERERQAEGAQSKADEKRPVAQKALYSTAMESIQGGAARGEAIETDDTMGGIPAVNGRGERLLLHIGIIDILQSYRFIKKLEHTWKALVHDGDTVSVHRPSFYAERFFKFMSSTVFRKSSSLKSSPSKKGRGALLAVKPLGPTAAFSASQIPSEREDVQYDLRGARSYPTLEDEGRPDLLPCTPPSFEEATTASIATTLSSTSLSIPERSPSDTSEQPRYRRRTQSSGQDGRPQEELHAEDLQKITVQVEPVCGVGVVVPKEQGAGVEVPPSGASAAATVEVDAASQASEPASQASDEEDAPSTDIYFPTDERSWVYSPLHYSARPASDGESDT

Target ORF information:

RefSeq Version	XM_006240935.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphatidylinositol-4-phosphate 5-kinase type 1 gamma (Pip5k1c), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006240935.3

ORF Insert Sequence:

ATGGAGCTAG AGGTGCCGGA CGAGGCAGAG AGCGCCGAAG CAGGGGCCGT GACAGCGGAG 
GCGGCCTGGT CTGCGGAGAG TGGGGCGGCG GCAGGTATGA CCCAGAAGAA GGCTATCCTT 
GCAGAGGCCC CGTTGGTGAC AGGGCAGCCA GGCCCTGGCC ATGGGAAGAA GCTGGGCCAT 
CGAGGCGTGG ATGCGTCTGG AGAGACTACG TATAAGAAGA CCACCTCGTC CACCCTGAAG 
GGTGCCATCC AACTGGGGAT TGGGTACACG GTGGGCAACC TCAGCTCTAA GCCAGAACGC 
GATGTGCTCA TGCAGGACTT CTATGTGGTG GAGAGCATCT TCTTTCCCAG CGAAGGGAGC 
AACCTTACCC CCGCCCACCA CTTCCAGGAT TTCCGTTTCA AGACCTATGC ACCTGTTGCC 
TTCCGTTACT TCCGGGAGCT CTTTGGTATC CGTCCGGACG ATTACTTGTA CTCACTCTGC 
AACGAGCCAC TCATTGAGCT GTCCAACCCG GGTGCCAGTG GCTCCGTCTT CTACGTCACC 
AGCGATGACG AGTTCATCAT TAAGACTGTC ATGCACAAGG AGGCGGAGTT CCTGCAGAAA 
CTGCTGCCTG GCTACTATAT GAACCTCAAC CAGAACCCCC GGACCTTGCT GCCCAAGTTC 
TATGGGCTGT ACTGTGTGCA GTCCGGCGGC AAGAACATTC GAGTGGTGGT CATGAACAAT 
GTGCTGCCCC GTGTCGTCAA GATGCACCTC AAGTTCGACC TCAAGGGCTC CACGTACAAG 
CGCAGGGCCA GCAAGAAGGA GAAGGAGAAG AGCCTGCCCA CCTACAAGGA CCTGGACTTC 
ATGCAGGACA TGCCAGAGGG GCTGCTACTG GACTCTGACA CCTTTGGCGC CCTGGTCAAG 
ACGCTGCAGC GAGACTGCCT GGTGTTGGAG AGCTTCAAGA TAATGGACTA CAGCCTGCTG 
CTGGGTGTGC ACAACATTGA CCAGCAGGAG CGGGAGCGCC AGGCCGAGGG CGCCCAGAGC 
AAGGCTGACG AGAAGCGGCC GGTGGCCCAG AAGGCCCTCT ACTCCACAGC CATGGAGTCC 
ATTCAGGGCG GAGCTGCCCG TGGGGAGGCC ATTGAGACAG ATGACACGAT GGGTGGGATC 
CCAGCCGTGA ATGGGCGTGG GGAGCGCTTG CTCCTACACA TCGGAATCAT TGACATTCTG 
CAGTCCTACA GGTTCATCAA GAAGTTAGAA CACACCTGGA AGGCCCTCGT CCACGATGGG 
GATACCGTCT CAGTCCACCG GCCCAGCTTC TATGCCGAGC GCTTCTTCAA GTTCATGAGC 
AGCACGGTCT TCCGGAAGAG TTCCTCCCTC AAGTCCTCTC CATCTAAGAA AGGGCGTGGC 
GCCCTGCTCG CAGTCAAGCC CCTGGGGCCC ACCGCAGCCT TCTCAGCTAG CCAGATCCCC 
AGTGAGAGAG AGGATGTGCA GTACGACCTG CGGGGAGCCC GCAGCTACCC CACGCTGGAG 
GATGAAGGCC GGCCTGACCT CCTGCCCTGC ACCCCACCGT CCTTTGAGGA AGCCACCACA 
GCCTCTATCG CCACCACCCT GTCATCCACC TCCCTCTCCA TCCCAGAGCG GTCCCCTTCA 
GACACATCAG AGCAGCCCCG GTACAGGCGG CGTACGCAGT CTTCAGGCCA GGATGGCCGG 
CCCCAGGAGG AGCTGCATGC GGAAGACCTG CAGAAGATAA CTGTGCAGGT GGAGCCAGTG 
TGTGGTGTGG GGGTTGTCGT CCCCAAGGAG CAGGGTGCAG GAGTGGAGGT TCCCCCATCT 
GGGGCATCGG CAGCAGCCAC GGTGGAAGTA GACGCTGCCA GCCAGGCCTC AGAGCCCGCC 
AGCCAAGCCT CTGATGAGGA AGATGCGCCT TCCACAGACA TCTATTTTCC CACCGACGAG 
AGGAGCTGGG TGTACTCCCC GCTTCACTAT AGCGCACGGC CCGCCTCCGA CGGCGAGAGC 
GACACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pip5k1c ( NM_001009967.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001009967.2 Rattus norvegicus phosphatidylinositol-4-phosphate 5-kinase type 1 gamma (Pip5k1c), transcript variant c, mRNA.

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CloneID	ORa01316
Clone ID Related Accession (Same CDS sequence)	NM_001009967.2
Accession Version	NM_001009967.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	phosphatidylinositol 4-phosphate 5-kinase type-1 gamma isoform c
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CB557931.1, AY850259.1, CK366332.1, BC101909.1, CB581409.1 and CB696331.1. On Aug 5, 2006 this sequence version replaced NM_001009967.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCTAG AGGTGCCGGA CGAGGCAGAG AGCGCCGAAG CAGGGGCCGT GACAGCGGAG 
GCGGCCTGGT CTGCGGAGAG TGGGGCGGCG GCAGGTATGA CCCAGAAGAA GGCTATCCTT 
GCAGAGGCCC CGTTGGTGAC AGGGCAGCCA GGCCCTGGCC ATGGGAAGAA GCTGGGCCAT 
CGAGGCGTGG ATGCGTCTGG AGAGACTACG TATAAGAAGA CCACCTCGTC CACCCTGAAG 
GGTGCCATCC AACTGGGGAT TGGGTACACG GTGGGCAACC TCAGCTCTAA GCCAGAACGC 
GATGTGCTCA TGCAGGACTT CTATGTGGTG GAGAGCATCT TCTTTCCCAG CGAAGGGAGC 
AACCTTACCC CCGCCCACCA CTTCCAGGAT TTCCGTTTCA AGACCTATGC ACCTGTTGCC 
TTCCGTTACT TCCGGGAGCT CTTTGGTATC CGTCCGGACG ATTACTTGTA CTCACTCTGC 
AACGAGCCAC TCATTGAGCT GTCCAACCCG GGTGCCAGTG GCTCCGTCTT CTACGTCACC 
AGCGATGACG AGTTCATCAT TAAGACTGTC ATGCACAAGG AGGCGGAGTT CCTGCAGAAA 
CTGCTGCCTG GCTACTATAT GAACCTCAAC CAGAACCCCC GGACCTTGCT GCCCAAGTTC 
TATGGGCTGT ACTGTGTGCA GTCCGGCGGC AAGAACATTC GAGTGGTGGT CATGAACAAT 
GTGCTGCCCC GTGTCGTCAA GATGCACCTC AAGTTCGACC TCAAGGGCTC CACGTACAAG 
CGCAGGGCCA GCAAGAAGGA GAAGGAGAAG AGCCTGCCCA CCTACAAGGA CCTGGACTTC 
ATGCAGGACA TGCCAGAGGG GCTGCTACTG GACTCTGACA CCTTTGGCGC CCTGGTCAAG 
ACGCTGCAGC GAGACTGCCT GGTGTTGGAG AGCTTCAAGA TAATGGACTA CAGCCTGCTG 
CTGGGTGTGC ACAACATTGA CCAGCAGGAG CGGGAGCGCC AGGCCGAGGG CGCCCAGAGC 
AAGGCTGACG AGAAGCGGCC GGTGGCCCAG AAGGCCCTCT ACTCCACAGC CATGGAGTCC 
ATTCAGGGCG GAGCTGCCCG TGGGGAGGCC ATTGAGACAG ATGACACGAT GGGTGGGATC 
CCAGCCGTGA ATGGGCGTGG GGAGCGCTTG CTCCTACACA TCGGAATCAT TGACATTCTG 
CAGTCCTACA GGTTCATCAA GAAGTTAGAA CACACCTGGA AGGCCCTCGT CCACGATGGG 
GATACCGTCT CAGTCCACCG GCCCAGCTTC TATGCCGAGC GCTTCTTCAA GTTCATGAGC 
AGCACGGTCT TCCGGAAGAG TTCCTCCCTC AAGTCCTCTC CATCTAAGAA AGGGCGTGGC 
GCCCTGCTCG CAGTCAAGCC CCTGGGGCCC ACCGCAGCCT TCTCAGCTAG CCAGATCCCC 
AGTGAGAGAG AGGATGTGCA GTACGACCTG CGGGGGGCCC GCAGCTACCC CACGCTGGAG 
GATGAAGGCC GGCCTGACCT CCTGCCCTGC ACCCCACCGT CCTTTGAGGA AGCCACCACA 
GCCTCTATCG CCACCACCCT GTCATCCACC TCCCTCTCCA TCCCAGAGCG GTCCCCTTCA 
GACACATCAG AGCAGCCCCG GTACAGGCGG CGTACGCAGT CTTCAGGCCA GGATGGCCGG 
CCCCAGGAGG AGCTGCATGC GGAAGACCTG CAGAAGATAA CTGTGCAGGT GGAGCCAGTG 
TGTGGTGTGG GGGTTGTCGT CCCCAAGGAG CAGGGTGCAG GAGTGGAGGT TCCCCCATCT 
GGGGCATCGG CAGCAGCCAC GGTGGAAGTA GACGCTGCCA GCCAGGCCTC AGAGCCCGCC 
AGCCAAGCCT CTGATGAGGA AGATGCGCCT TCCACAGACA TCTATTTTTT CGCCCACGGG 
AGATACTGGC TTTTCTCTCC CCGTCGTCGC CGACTGCGGG CCGTGACACC AAGCCACACA 
GGCGCTCCCA CCGACGAGAG GAGCTGGGTG TACTCCCCGC TTCACTATAG CGCACGGCCC 
GCCTCCGACG GCGAGAGCGA CACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001009967.2
CDS	62..2128
Translation	MELEVPDEAESAEAGAVTAEAAWSAESGAAAGMTQKKAILAEAPLVTGQPGPGHGKKLGHRGVDASGETTYKKTTSSTLKGAIQLGIGYTVGNLSSKPERDVLMQDFYVVESIFFPSEGSNLTPAHHFQDFRFKTYAPVAFRYFRELFGIRPDDYLYSLCNEPLIELSNPGASGSVFYVTSDDEFIIKTVMHKEAEFLQKLLPGYYMNLNQNPRTLLPKFYGLYCVQSGGKNIRVVVMNNVLPRVVKMHLKFDLKGSTYKRRASKKEKEKSLPTYKDLDFMQDMPEGLLLDSDTFGALVKTLQRDCLVLESFKIMDYSLLLGVHNIDQQERERQAEGAQSKADEKRPVAQKALYSTAMESIQGGAARGEAIETDDTMGGIPAVNGRGERLLLHIGIIDILQSYRFIKKLEHTWKALVHDGDTVSVHRPSFYAERFFKFMSSTVFRKSSSLKSSPSKKGRGALLAVKPLGPTAAFSASQIPSEREDVQYDLRGARSYPTLEDEGRPDLLPCTPPSFEEATTASIATTLSSTSLSIPERSPSDTSEQPRYRRRTQSSGQDGRPQEELHAEDLQKITVQVEPVCGVGVVVPKEQGAGVEVPPSGASAAATVEVDAASQASEPASQASDEEDAPSTDIYFFAHGRYWLFSPRRRRLRAVTPSHTGAPTDERSWVYSPLHYSARPASDGESDT

Target ORF information:

RefSeq Version	NM_001009967.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphatidylinositol-4-phosphate 5-kinase type 1 gamma (Pip5k1c), transcript variant c, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001009967.2

ORF Insert Sequence:

ATGGAGCTAG AGGTGCCGGA CGAGGCAGAG AGCGCCGAAG CAGGGGCCGT GACAGCGGAG 
GCGGCCTGGT CTGCGGAGAG TGGGGCGGCG GCAGGTATGA CCCAGAAGAA GGCTATCCTT 
GCAGAGGCCC CGTTGGTGAC AGGGCAGCCA GGCCCTGGCC ATGGGAAGAA GCTGGGCCAT 
CGAGGCGTGG ATGCGTCTGG AGAGACTACG TATAAGAAGA CCACCTCGTC CACCCTGAAG 
GGTGCCATCC AACTGGGGAT TGGGTACACG GTGGGCAACC TCAGCTCTAA GCCAGAACGC 
GATGTGCTCA TGCAGGACTT CTATGTGGTG GAGAGCATCT TCTTTCCCAG CGAAGGGAGC 
AACCTTACCC CCGCCCACCA CTTCCAGGAT TTCCGTTTCA AGACCTATGC ACCTGTTGCC 
TTCCGTTACT TCCGGGAGCT CTTTGGTATC CGTCCGGACG ATTACTTGTA CTCACTCTGC 
AACGAGCCAC TCATTGAGCT GTCCAACCCG GGTGCCAGTG GCTCCGTCTT CTACGTCACC 
AGCGATGACG AGTTCATCAT TAAGACTGTC ATGCACAAGG AGGCGGAGTT CCTGCAGAAA 
CTGCTGCCTG GCTACTATAT GAACCTCAAC CAGAACCCCC GGACCTTGCT GCCCAAGTTC 
TATGGGCTGT ACTGTGTGCA GTCCGGCGGC AAGAACATTC GAGTGGTGGT CATGAACAAT 
GTGCTGCCCC GTGTCGTCAA GATGCACCTC AAGTTCGACC TCAAGGGCTC CACGTACAAG 
CGCAGGGCCA GCAAGAAGGA GAAGGAGAAG AGCCTGCCCA CCTACAAGGA CCTGGACTTC 
ATGCAGGACA TGCCAGAGGG GCTGCTACTG GACTCTGACA CCTTTGGCGC CCTGGTCAAG 
ACGCTGCAGC GAGACTGCCT GGTGTTGGAG AGCTTCAAGA TAATGGACTA CAGCCTGCTG 
CTGGGTGTGC ACAACATTGA CCAGCAGGAG CGGGAGCGCC AGGCCGAGGG CGCCCAGAGC 
AAGGCTGACG AGAAGCGGCC GGTGGCCCAG AAGGCCCTCT ACTCCACAGC CATGGAGTCC 
ATTCAGGGCG GAGCTGCCCG TGGGGAGGCC ATTGAGACAG ATGACACGAT GGGTGGGATC 
CCAGCCGTGA ATGGGCGTGG GGAGCGCTTG CTCCTACACA TCGGAATCAT TGACATTCTG 
CAGTCCTACA GGTTCATCAA GAAGTTAGAA CACACCTGGA AGGCCCTCGT CCACGATGGG 
GATACCGTCT CAGTCCACCG GCCCAGCTTC TATGCCGAGC GCTTCTTCAA GTTCATGAGC 
AGCACGGTCT TCCGGAAGAG TTCCTCCCTC AAGTCCTCTC CATCTAAGAA AGGGCGTGGC 
GCCCTGCTCG CAGTCAAGCC CCTGGGGCCC ACCGCAGCCT TCTCAGCTAG CCAGATCCCC 
AGTGAGAGAG AGGATGTGCA GTACGACCTG CGGGGGGCCC GCAGCTACCC CACGCTGGAG 
GATGAAGGCC GGCCTGACCT CCTGCCCTGC ACCCCACCGT CCTTTGAGGA AGCCACCACA 
GCCTCTATCG CCACCACCCT GTCATCCACC TCCCTCTCCA TCCCAGAGCG GTCCCCTTCA 
GACACATCAG AGCAGCCCCG GTACAGGCGG CGTACGCAGT CTTCAGGCCA GGATGGCCGG 
CCCCAGGAGG AGCTGCATGC GGAAGACCTG CAGAAGATAA CTGTGCAGGT GGAGCCAGTG 
TGTGGTGTGG GGGTTGTCGT CCCCAAGGAG CAGGGTGCAG GAGTGGAGGT TCCCCCATCT 
GGGGCATCGG CAGCAGCCAC GGTGGAAGTA GACGCTGCCA GCCAGGCCTC AGAGCCCGCC 
AGCCAAGCCT CTGATGAGGA AGATGCGCCT TCCACAGACA TCTATTTTTT CGCCCACGGG 
AGATACTGGC TTTTCTCTCC CCGTCGTCGC CGACTGCGGG CCGTGACACC AAGCCACACA 
GGCGCTCCCA CCGACGAGAG GAGCTGGGTG TACTCCCCGC TTCACTATAG CGCACGGCCC 
GCCTCCGACG GCGAGAGCGA CACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pip5k1c ( XM_008765101.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008763323.1 Rattus norvegicus phosphatidylinositol-4-phosphate 5-kinase type 1 gamma (Pip5k1c), transcript variant X1, mRNA.

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CloneID	ORa47339
Clone ID Related Accession (Same CDS sequence)	XM_008765101.2
Accession Version	XM_008765101.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	phosphatidylinositol 4-phosphate 5-kinase type-1 gamma isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008765101.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTAG AGGTGCCGGA CGAGGCAGAG AGCGCCGAAG CAGGGGCCGT GACAGCGGAG 
GCGGCCTGGT CTGCGGAGAG TGGGGCGGCG GCAGGTATGA CCCAGAAGAA GGCTATCCTT 
GCAGAGGCCC CGTTGGTGAC AGGGCAGCCA GGCCCTGGCC ATGGGAAGAA GCTGGGCCAT 
CGAGGCGTGG ATGCGTCTGG AGAGACTACG TATAAGAAGA CCACCTCGTC CACCCTGAAG 
GGTGCCATCC AACTGGGGAT TGGGTACACG GTGGGCAACC TCAGCTCTAA GCCAGAACGC 
GATGTGCTCA TGCAGGACTT CTATGTGGTG GAGAGCATCT TCTTTCCCAG CGAAGGGAGC 
AACCTTACCC CCGCCCACCA CTTCCAGGAT TTCCGTTTCA AGACCTATGC ACCTGTTGCC 
TTCCGTTACT TCCGGGAGCT CTTTGGTATC CGTCCGGACG ATTACTTGTA CTCACTCTGC 
AACGAGCCAC TCATTGAGCT GTCCAACCCG GGTGCCAGTG GCTCCGTCTT CTACGTCACC 
AGCGATGACG AGTTCATCAT TAAGACTGTC ATGCACAAGG AGGCGGAGTT CCTGCAGAAA 
CTGCTGCCTG GCTACTATAT GAACCTCAAC CAGAACCCCC GGACCTTGCT GCCCAAGTTC 
TATGGGCTGT ACTGTGTGCA GTCCGGCGGC AAGAACATTC GAGTGGTGGT CATGAACAAT 
GTGCTGCCCC GTGTCGTCAA GATGCACCTC AAGTTCGACC TCAAGGGCTC CACGTACAAG 
CGCAGGGCCA GCAAGAAGGA GAAGGAGAAG AGCCTGCCCA CCTACAAGGA CCTGGACTTC 
ATGCAGGACA TGCCAGAGGG GCTGCTACTG GACTCTGACA CCTTTGGCGC CCTGGTCAAG 
ACGCTGCAGC GAGACTGCCT GGTGTTGGAG AGCTTCAAGA TAATGGACTA CAGCCTGCTG 
CTGGGTGTGC ACAACATTGA CCAGCAGGAG CGGGAGCGCC AGGCCGAGGG CGCCCAGAGC 
AAGGCTGACG AGAAGCGGCC GGTGGCCCAG AAGGCCCTCT ACTCCACAGC CATGGAGTCC 
ATTCAGGGCG GAGCTGCCCG TGGGGAGGCC ATTGAGACAG ATGACACGAT GGGTGGGATC 
CCAGCCGTGA ATGGGCGTGG GGAGCGCTTG CTCCTACACA TCGGAATCAT TGACATTCTG 
CAGTCCTACA GGTTCATCAA GAAGTTAGAA CACACCTGGA AGGCCCTCGT CCACGATGGG 
GATACCGTCT CAGTCCACCG GCCCAGCTTC TATGCCGAGC GCTTCTTCAA GTTCATGAGC 
AGCACGGTCT TCCGGAAGAG TTCCTCCCTC AAGTCCTCTC CATCTAAGAA AGGGCGTGGC 
GCCCTGCTCG CAGTCAAGCC CCTGGGGCCC ACCGCAGCCT TCTCAGCTAG CCAGATCCCC 
AGTGAGAGAG AGGATGTGCA GTACGACCTG CGGGGAGCCC GCAGCTACCC CACGCTGGAG 
GATGAAGGCC GGCCTGACCT CCTGCCCTGC ACCCCACCGT CCTTTGAGGA AGCCACCACA 
GCCTCTATCG CCACCACCCT GTCATCCACC TCCCTCTCCA TCCCAGAGCG GTCCCCTTCA 
GACACATCAG AGCAGCCCCG GTACAGGCGG CGTACGCAGT CTTCAGGCCA GGATGGCCGG 
CCCCAGGAGG AGCTGCATGC GGAAGACCTG CAGAAGATAA CTGTGCAGGT GGAGCCAGTG 
TGTGGTGTGG GGGTTGTCGT CCCCAAGGAG CAGGGTGCAG GAGTGGAGGT TCCCCCATCT 
GGGGCATCGG CAGCAGCCAC GGTGGAAGTA GACGCTGCCA GCCAGGCCTC AGAGCCCGCC 
AGCCAAGCCT CTGATGAGGA AGATGCGCCT TCCACAGACA TCTATTTTTT CGCCCACGGG 
AGATACTGGC TTTTCTCTCC CCGTCGTCGC CAACTGCGGG CCGTGACACC AAGCCACACA 
GGCGCTCCCA CCGACGAGAG GAGCTGGGTG TACTCCCCGC TTCACTATAG CGCACGGCCC 
GCCTCCGACG GCGAGAGCGA CACAGTAAGT CCCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008763323.1
CDS	104..2182
Translation	MELEVPDEAESAEAGAVTAEAAWSAESGAAAGMTQKKAILAEAPLVTGQPGPGHGKKLGHRGVDASGETTYKKTTSSTLKGAIQLGIGYTVGNLSSKPERDVLMQDFYVVESIFFPSEGSNLTPAHHFQDFRFKTYAPVAFRYFRELFGIRPDDYLYSLCNEPLIELSNPGASGSVFYVTSDDEFIIKTVMHKEAEFLQKLLPGYYMNLNQNPRTLLPKFYGLYCVQSGGKNIRVVVMNNVLPRVVKMHLKFDLKGSTYKRRASKKEKEKSLPTYKDLDFMQDMPEGLLLDSDTFGALVKTLQRDCLVLESFKIMDYSLLLGVHNIDQQERERQAEGAQSKADEKRPVAQKALYSTAMESIQGGAARGEAIETDDTMGGIPAVNGRGERLLLHIGIIDILQSYRFIKKLEHTWKALVHDGDTVSVHRPSFYAERFFKFMSSTVFRKSSSLKSSPSKKGRGALLAVKPLGPTAAFSASQIPSEREDVQYDLRGARSYPTLEDEGRPDLLPCTPPSFEEATTASIATTLSSTSLSIPERSPSDTSEQPRYRRRTQSSGQDGRPQEELHAEDLQKITVQVEPVCGVGVVVPKEQGAGVEVPPSGASAAATVEVDAASQASEPASQASDEEDAPSTDIYFFAHGRYWLFSPRRRQLRAVTPSHTGAPTDERSWVYSPLHYSARPASDGESDTVSPQ

Target ORF information:

RefSeq Version	XM_008765101.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphatidylinositol-4-phosphate 5-kinase type 1 gamma (Pip5k1c), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765101.2

ORF Insert Sequence:

ATGGAGCTAG AGGTGCCGGA CGAGGCAGAG AGCGCCGAAG CAGGGGCCGT GACAGCGGAG 
GCGGCCTGGT CTGCGGAGAG TGGGGCGGCG GCAGGTATGA CCCAGAAGAA GGCTATCCTT 
GCAGAGGCCC CGTTGGTGAC AGGGCAGCCA GGCCCTGGCC ATGGGAAGAA GCTGGGCCAT 
CGAGGCGTGG ATGCGTCTGG AGAGACTACG TATAAGAAGA CCACCTCGTC CACCCTGAAG 
GGTGCCATCC AACTGGGGAT TGGGTACACG GTGGGCAACC TCAGCTCTAA GCCAGAACGC 
GATGTGCTCA TGCAGGACTT CTATGTGGTG GAGAGCATCT TCTTTCCCAG CGAAGGGAGC 
AACCTTACCC CCGCCCACCA CTTCCAGGAT TTCCGTTTCA AGACCTATGC ACCTGTTGCC 
TTCCGTTACT TCCGGGAGCT CTTTGGTATC CGTCCGGACG ATTACTTGTA CTCACTCTGC 
AACGAGCCAC TCATTGAGCT GTCCAACCCG GGTGCCAGTG GCTCCGTCTT CTACGTCACC 
AGCGATGACG AGTTCATCAT TAAGACTGTC ATGCACAAGG AGGCGGAGTT CCTGCAGAAA 
CTGCTGCCTG GCTACTATAT GAACCTCAAC CAGAACCCCC GGACCTTGCT GCCCAAGTTC 
TATGGGCTGT ACTGTGTGCA GTCCGGCGGC AAGAACATTC GAGTGGTGGT CATGAACAAT 
GTGCTGCCCC GTGTCGTCAA GATGCACCTC AAGTTCGACC TCAAGGGCTC CACGTACAAG 
CGCAGGGCCA GCAAGAAGGA GAAGGAGAAG AGCCTGCCCA CCTACAAGGA CCTGGACTTC 
ATGCAGGACA TGCCAGAGGG GCTGCTACTG GACTCTGACA CCTTTGGCGC CCTGGTCAAG 
ACGCTGCAGC GAGACTGCCT GGTGTTGGAG AGCTTCAAGA TAATGGACTA CAGCCTGCTG 
CTGGGTGTGC ACAACATTGA CCAGCAGGAG CGGGAGCGCC AGGCCGAGGG CGCCCAGAGC 
AAGGCTGACG AGAAGCGGCC GGTGGCCCAG AAGGCCCTCT ACTCCACAGC CATGGAGTCC 
ATTCAGGGCG GAGCTGCCCG TGGGGAGGCC ATTGAGACAG ATGACACGAT GGGTGGGATC 
CCAGCCGTGA ATGGGCGTGG GGAGCGCTTG CTCCTACACA TCGGAATCAT TGACATTCTG 
CAGTCCTACA GGTTCATCAA GAAGTTAGAA CACACCTGGA AGGCCCTCGT CCACGATGGG 
GATACCGTCT CAGTCCACCG GCCCAGCTTC TATGCCGAGC GCTTCTTCAA GTTCATGAGC 
AGCACGGTCT TCCGGAAGAG TTCCTCCCTC AAGTCCTCTC CATCTAAGAA AGGGCGTGGC 
GCCCTGCTCG CAGTCAAGCC CCTGGGGCCC ACCGCAGCCT TCTCAGCTAG CCAGATCCCC 
AGTGAGAGAG AGGATGTGCA GTACGACCTG CGGGGAGCCC GCAGCTACCC CACGCTGGAG 
GATGAAGGCC GGCCTGACCT CCTGCCCTGC ACCCCACCGT CCTTTGAGGA AGCCACCACA 
GCCTCTATCG CCACCACCCT GTCATCCACC TCCCTCTCCA TCCCAGAGCG GTCCCCTTCA 
GACACATCAG AGCAGCCCCG GTACAGGCGG CGTACGCAGT CTTCAGGCCA GGATGGCCGG 
CCCCAGGAGG AGCTGCATGC GGAAGACCTG CAGAAGATAA CTGTGCAGGT GGAGCCAGTG 
TGTGGTGTGG GGGTTGTCGT CCCCAAGGAG CAGGGTGCAG GAGTGGAGGT TCCCCCATCT 
GGGGCATCGG CAGCAGCCAC GGTGGAAGTA GACGCTGCCA GCCAGGCCTC AGAGCCCGCC 
AGCCAAGCCT CTGATGAGGA AGATGCGCCT TCCACAGACA TCTATTTTTT CGCCCACGGG 
AGATACTGGC TTTTCTCTCC CCGTCGTCGC CAACTGCGGG CCGTGACACC AAGCCACACA 
GGCGCTCCCA CCGACGAGAG GAGCTGGGTG TACTCCCCGC TTCACTATAG CGCACGGCCC 
GCCTCCGACG GCGAGAGCGA CACAGTAAGT CCCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pip5k1c ( XM_008765103.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008763325.1 Rattus norvegicus phosphatidylinositol-4-phosphate 5-kinase type 1 gamma (Pip5k1c), transcript variant X2, mRNA.

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CloneID	ORa47341
Clone ID Related Accession (Same CDS sequence)	XM_008765103.2
Accession Version	XM_008765103.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2001bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	phosphatidylinositol 4-phosphate 5-kinase type-1 gamma isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008765103.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTAG AGGTGCCGGA CGAGGCAGAG AGCGCCGAAG CAGGGGCCGT GACAGCGGAG 
GCGGCCTGGT CTGCGGAGAG TGGGGCGGCG GCAGGTATGA CCCAGAAGAA GGCTATCCTT 
GCAGAGGCCC CGTTGGTGAC AGGGCAGCCA GGCCCTGGCC ATGGGAAGAA GCTGGGCCAT 
CGAGGCGTGG ATGCGTCTGG AGAGACTACG TATAAGAAGA CCACCTCGTC CACCCTGAAG 
GGTGCCATCC AACTGGGGAT TGGGTACACG GTGGGCAACC TCAGCTCTAA GCCAGAACGC 
GATGTGCTCA TGCAGGACTT CTATGTGGTG GAGAGCATCT TCTTTCCCAG CGAAGGGAGC 
AACCTTACCC CCGCCCACCA CTTCCAGGAT TTCCGTTTCA AGACCTATGC ACCTGTTGCC 
TTCCGTTACT TCCGGGAGCT CTTTGGTATC CGTCCGGACG ATTACTTGTA CTCACTCTGC 
AACGAGCCAC TCATTGAGCT GTCCAACCCG GGTGCCAGTG GCTCCGTCTT CTACGTCACC 
AGCGATGACG AGTTCATCAT TAAGACTGTC ATGCACAAGG AGGCGGAGTT CCTGCAGAAA 
CTGCTGCCTG GCTACTATAT GAACCTCAAC CAGAACCCCC GGACCTTGCT GCCCAAGTTC 
TATGGGCTGT ACTGTGTGCA GTCCGGCGGC AAGAACATTC GAGTGGTGGT CATGAACAAT 
GTGCTGCCCC GTGTCGTCAA GATGCACCTC AAGTTCGACC TCAAGGGCTC CACGTACAAG 
CGCAGGGCCA GCAAGAAGGA GAAGGAGAAG AGCCTGCCCA CCTACAAGGA CCTGGACTTC 
ATGCAGGACA TGCCAGAGGG GCTGCTACTG GACTCTGACA CCTTTGGCGC CCTGGTCAAG 
ACGCTGCAGC GAGACTGCCT GGTGTTGGAG AGCTTCAAGA TAATGGACTA CAGCCTGCTG 
CTGGGTGTGC ACAACATTGA CCAGCAGGAG CGGGAGCGCC AGGCCGAGGG CGCCCAGAGC 
AAGGCTGACG AGAAGCGGCC GGTGGCCCAG AAGGCCCTCT ACTCCACAGC CATGGAGTCC 
ATTCAGGGCG GAGCTGCCCG TGGGGAGGCC ATTGAGACAG ATGACACGAT GGGTGGGATC 
CCAGCCGTGA ATGGGCGTGG GGAGCGCTTG CTCCTACACA TCGGAATCAT TGACATTCTG 
CAGTCCTACA GGTTCATCAA GAAGTTAGAA CACACCTGGA AGGCCCTCGT CCACGATGGG 
GATACCGTCT CAGTCCACCG GCCCAGCTTC TATGCCGAGC GCTTCTTCAA GTTCATGAGC 
AGCACGGTCT TCCGGAAGAG TTCCTCCCTC AAGTCCTCTC CATCTAAGAA AGGGCGTGGC 
GCCCTGCTCG CAGTCAAGCC CCTGGGGCCC ACCGCAGCCT TCTCAGCTAG CCAGATCCCC 
AGTGAGAGAG AGGATGTGCA GTACGACCTG CGGGGAGCCC GCAGCTACCC CACGCTGGAG 
GATGAAGGCC GGCCTGACCT CCTGCCCTGC ACCCCACCGT CCTTTGAGGA AGCCACCACA 
GCCTCTATCG CCACCACCCT GTCATCCACC TCCCTCTCCA TCCCAGAGCG GTCCCCTTCA 
GACACATCAG AGCAGCCCCG GTACAGGCGG CGTACGCAGT CTTCAGGCCA GGATGGCCGG 
CCCCAGGAGG AGCTGCATGC GGAAGACCTG CAGAAGATAA CTGTGCAGGT GGAGCCAGTG 
TGTGGTGTGG GGGTTGTCGT CCCCAAGGAG CAGGGTGCAG GAGTGGAGGT TCCCCCATCT 
GGGGCATCGG CAGCAGCCAC GGTGGAAGTA GACGCTGCCA GCCAGGCCTC AGAGCCCGCC 
AGCCAAGCCT CTGATGAGGA AGATGCGCCT TCCACAGACA TCTATTTTCC CACCGACGAG 
AGGAGCTGGG TGTACTCCCC GCTTCACTAT AGCGCACGGC CCGCCTCCGA CGGCGAGAGC 
GACACAGTAA GTCCCCAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008763325.1
CDS	103..2103
Translation	MELEVPDEAESAEAGAVTAEAAWSAESGAAAGMTQKKAILAEAPLVTGQPGPGHGKKLGHRGVDASGETTYKKTTSSTLKGAIQLGIGYTVGNLSSKPERDVLMQDFYVVESIFFPSEGSNLTPAHHFQDFRFKTYAPVAFRYFRELFGIRPDDYLYSLCNEPLIELSNPGASGSVFYVTSDDEFIIKTVMHKEAEFLQKLLPGYYMNLNQNPRTLLPKFYGLYCVQSGGKNIRVVVMNNVLPRVVKMHLKFDLKGSTYKRRASKKEKEKSLPTYKDLDFMQDMPEGLLLDSDTFGALVKTLQRDCLVLESFKIMDYSLLLGVHNIDQQERERQAEGAQSKADEKRPVAQKALYSTAMESIQGGAARGEAIETDDTMGGIPAVNGRGERLLLHIGIIDILQSYRFIKKLEHTWKALVHDGDTVSVHRPSFYAERFFKFMSSTVFRKSSSLKSSPSKKGRGALLAVKPLGPTAAFSASQIPSEREDVQYDLRGARSYPTLEDEGRPDLLPCTPPSFEEATTASIATTLSSTSLSIPERSPSDTSEQPRYRRRTQSSGQDGRPQEELHAEDLQKITVQVEPVCGVGVVVPKEQGAGVEVPPSGASAAATVEVDAASQASEPASQASDEEDAPSTDIYFPTDERSWVYSPLHYSARPASDGESDTVSPQ

Target ORF information:

RefSeq Version	XM_008765103.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphatidylinositol-4-phosphate 5-kinase type 1 gamma (Pip5k1c), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765103.2

ORF Insert Sequence:

ATGGAGCTAG AGGTGCCGGA CGAGGCAGAG AGCGCCGAAG CAGGGGCCGT GACAGCGGAG 
GCGGCCTGGT CTGCGGAGAG TGGGGCGGCG GCAGGTATGA CCCAGAAGAA GGCTATCCTT 
GCAGAGGCCC CGTTGGTGAC AGGGCAGCCA GGCCCTGGCC ATGGGAAGAA GCTGGGCCAT 
CGAGGCGTGG ATGCGTCTGG AGAGACTACG TATAAGAAGA CCACCTCGTC CACCCTGAAG 
GGTGCCATCC AACTGGGGAT TGGGTACACG GTGGGCAACC TCAGCTCTAA GCCAGAACGC 
GATGTGCTCA TGCAGGACTT CTATGTGGTG GAGAGCATCT TCTTTCCCAG CGAAGGGAGC 
AACCTTACCC CCGCCCACCA CTTCCAGGAT TTCCGTTTCA AGACCTATGC ACCTGTTGCC 
TTCCGTTACT TCCGGGAGCT CTTTGGTATC CGTCCGGACG ATTACTTGTA CTCACTCTGC 
AACGAGCCAC TCATTGAGCT GTCCAACCCG GGTGCCAGTG GCTCCGTCTT CTACGTCACC 
AGCGATGACG AGTTCATCAT TAAGACTGTC ATGCACAAGG AGGCGGAGTT CCTGCAGAAA 
CTGCTGCCTG GCTACTATAT GAACCTCAAC CAGAACCCCC GGACCTTGCT GCCCAAGTTC 
TATGGGCTGT ACTGTGTGCA GTCCGGCGGC AAGAACATTC GAGTGGTGGT CATGAACAAT 
GTGCTGCCCC GTGTCGTCAA GATGCACCTC AAGTTCGACC TCAAGGGCTC CACGTACAAG 
CGCAGGGCCA GCAAGAAGGA GAAGGAGAAG AGCCTGCCCA CCTACAAGGA CCTGGACTTC 
ATGCAGGACA TGCCAGAGGG GCTGCTACTG GACTCTGACA CCTTTGGCGC CCTGGTCAAG 
ACGCTGCAGC GAGACTGCCT GGTGTTGGAG AGCTTCAAGA TAATGGACTA CAGCCTGCTG 
CTGGGTGTGC ACAACATTGA CCAGCAGGAG CGGGAGCGCC AGGCCGAGGG CGCCCAGAGC 
AAGGCTGACG AGAAGCGGCC GGTGGCCCAG AAGGCCCTCT ACTCCACAGC CATGGAGTCC 
ATTCAGGGCG GAGCTGCCCG TGGGGAGGCC ATTGAGACAG ATGACACGAT GGGTGGGATC 
CCAGCCGTGA ATGGGCGTGG GGAGCGCTTG CTCCTACACA TCGGAATCAT TGACATTCTG 
CAGTCCTACA GGTTCATCAA GAAGTTAGAA CACACCTGGA AGGCCCTCGT CCACGATGGG 
GATACCGTCT CAGTCCACCG GCCCAGCTTC TATGCCGAGC GCTTCTTCAA GTTCATGAGC 
AGCACGGTCT TCCGGAAGAG TTCCTCCCTC AAGTCCTCTC CATCTAAGAA AGGGCGTGGC 
GCCCTGCTCG CAGTCAAGCC CCTGGGGCCC ACCGCAGCCT TCTCAGCTAG CCAGATCCCC 
AGTGAGAGAG AGGATGTGCA GTACGACCTG CGGGGAGCCC GCAGCTACCC CACGCTGGAG 
GATGAAGGCC GGCCTGACCT CCTGCCCTGC ACCCCACCGT CCTTTGAGGA AGCCACCACA 
GCCTCTATCG CCACCACCCT GTCATCCACC TCCCTCTCCA TCCCAGAGCG GTCCCCTTCA 
GACACATCAG AGCAGCCCCG GTACAGGCGG CGTACGCAGT CTTCAGGCCA GGATGGCCGG 
CCCCAGGAGG AGCTGCATGC GGAAGACCTG CAGAAGATAA CTGTGCAGGT GGAGCCAGTG 
TGTGGTGTGG GGGTTGTCGT CCCCAAGGAG CAGGGTGCAG GAGTGGAGGT TCCCCCATCT 
GGGGCATCGG CAGCAGCCAC GGTGGAAGTA GACGCTGCCA GCCAGGCCTC AGAGCCCGCC 
AGCCAAGCCT CTGATGAGGA AGATGCGCCT TCCACAGACA TCTATTTTCC CACCGACGAG 
AGGAGCTGGG TGTACTCCCC GCTTCACTAT AGCGCACGGC CCGCCTCCGA CGGCGAGAGC 
GACACAGTAA GTCCCCAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pip5k1c ( XM_017594818.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450307.1 Rattus norvegicus phosphatidylinositol-4-phosphate 5-kinase type 1 gamma (Pip5k1c), transcript variant X4, mRNA.

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CloneID	ORa61990
Clone ID Related Accession (Same CDS sequence)	XM_017594818.1
Accession Version	XM_017594818.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1983bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	phosphatidylinositol 4-phosphate 5-kinase type-1 gamma isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCAGA AGAAGGCTAT CCTTGCAGAG GCCCCGTTGG TGACAGGGCA GCCAGGCCCT 
GGCCATGGGA AGAAGCTGGG CCATCGAGGC GTGGATGCGT CTGGAGAGAC TACGTATAAG 
AAGACCACCT CGTCCACCCT GAAGGGTGCC ATCCAACTGG GGATTGGGTA CACGGTGGGC 
AACCTCAGCT CTAAGCCAGA ACGCGATGTG CTCATGCAGG ACTTCTATGT GGTGGAGAGC 
ATCTTCTTTC CCAGCGAAGG GAGCAACCTT ACCCCCGCCC ACCACTTCCA GGATTTCCGT 
TTCAAGACCT ATGCACCTGT TGCCTTCCGT TACTTCCGGG AGCTCTTTGG TATCCGTCCG 
GACGATTACT TGTACTCACT CTGCAACGAG CCACTCATTG AGCTGTCCAA CCCGGGTGCC 
AGTGGCTCCG TCTTCTACGT CACCAGCGAT GACGAGTTCA TCATTAAGAC TGTCATGCAC 
AAGGAGGCGG AGTTCCTGCA GAAACTGCTG CCTGGCTACT ATATGAACCT CAACCAGAAC 
CCCCGGACCT TGCTGCCCAA GTTCTATGGG CTGTACTGTG TGCAGTCCGG CGGCAAGAAC 
ATTCGAGTGG TGGTCATGAA CAATGTGCTG CCCCGTGTCG TCAAGATGCA CCTCAAGTTC 
GACCTCAAGG GCTCCACGTA CAAGCGCAGG GCCAGCAAGA AGGAGAAGGA GAAGAGCCTG 
CCCACCTACA AGGACCTGGA CTTCATGCAG GACATGCCAG AGGGGCTGCT ACTGGACTCT 
GACACCTTTG GCGCCCTGGT CAAGACGCTG CAGCGAGACT GCCTGGTGTT GGAGAGCTTC 
AAGATAATGG ACTACAGCCT GCTGCTGGGT GTGCACAACA TTGACCAGCA GGAGCGGGAG 
CGCCAGGCCG AGGGCGCCCA GAGCAAGGCT GACGAGAAGC GGCCGGTGGC CCAGAAGGCC 
CTCTACTCCA CAGCCATGGA GTCCATTCAG GGCGGAGCTG CCCGTGGGGA GGCCATTGAG 
ACAGATGACA CGATGGGTGG GATCCCAGCC GTGAATGGGC GTGGGGAGCG CTTGCTCCTA 
CACATCGGAA TCATTGACAT TCTGCAGTCC TACAGGTTCA TCAAGAAGTT AGAACACACC 
TGGAAGGCCC TCGTCCACGA TGGGGATACC GTCTCAGTCC ACCGGCCCAG CTTCTATGCC 
GAGCGCTTCT TCAAGTTCAT GAGCAGCACG GTCTTCCGGA AGAGTTCCTC CCTCAAGTCC 
TCTCCATCTA AGAAAGGGCG TGGCGCCCTG CTCGCAGTCA AGCCCCTGGG GCCCACCGCA 
GCCTTCTCAG CTAGCCAGAT CCCCAGTGAG AGAGAGGATG TGCAGTACGA CCTGCGGGGA 
GCCCGCAGCT ACCCCACGCT GGAGGATGAA GGCCGGCCTG ACCTCCTGCC CTGCACCCCA 
CCGTCCTTTG AGGAAGCCAC CACAGCCTCT ATCGCCACCA CCCTGTCATC CACCTCCCTC 
TCCATCCCAG AGCGGTCCCC TTCAGACACA TCAGAGCAGC CCCGGTACAG GCGGCGTACG 
CAGTCTTCAG GCCAGGATGG CCGGCCCCAG GAGGAGCTGC ATGCGGAAGA CCTGCAGAAG 
ATAACTGTGC AGGTGGAGCC AGTGTGTGGT GTGGGGGTTG TCGTCCCCAA GGAGCAGGGT 
GCAGGAGTGG AGGTTCCCCC ATCTGGGGCA TCGGCAGCAG CCACGGTGGA AGTAGACGCT 
GCCAGCCAGG CCTCAGAGCC CGCCAGCCAA GCCTCTGATG AGGAAGATGC GCCTTCCACA 
GACATCTATT TTTTCGCCCA CGGGAGATAC TGGCTTTTCT CTCCCCGTCG TCGCCAACTG 
CGGGCCGTGA CACCAAGCCA CACAGGCGCT CCCACCGACG AGAGGAGCTG GGTGTACTCC 
CCGCTTCACT ATAGCGCACG GCCCGCCTCC GACGGCGAGA GCGACACAGT AAGTCCCCAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450307.1
CDS	261..2243
Translation	MTQKKAILAEAPLVTGQPGPGHGKKLGHRGVDASGETTYKKTTSSTLKGAIQLGIGYTVGNLSSKPERDVLMQDFYVVESIFFPSEGSNLTPAHHFQDFRFKTYAPVAFRYFRELFGIRPDDYLYSLCNEPLIELSNPGASGSVFYVTSDDEFIIKTVMHKEAEFLQKLLPGYYMNLNQNPRTLLPKFYGLYCVQSGGKNIRVVVMNNVLPRVVKMHLKFDLKGSTYKRRASKKEKEKSLPTYKDLDFMQDMPEGLLLDSDTFGALVKTLQRDCLVLESFKIMDYSLLLGVHNIDQQERERQAEGAQSKADEKRPVAQKALYSTAMESIQGGAARGEAIETDDTMGGIPAVNGRGERLLLHIGIIDILQSYRFIKKLEHTWKALVHDGDTVSVHRPSFYAERFFKFMSSTVFRKSSSLKSSPSKKGRGALLAVKPLGPTAAFSASQIPSEREDVQYDLRGARSYPTLEDEGRPDLLPCTPPSFEEATTASIATTLSSTSLSIPERSPSDTSEQPRYRRRTQSSGQDGRPQEELHAEDLQKITVQVEPVCGVGVVVPKEQGAGVEVPPSGASAAATVEVDAASQASEPASQASDEEDAPSTDIYFFAHGRYWLFSPRRRQLRAVTPSHTGAPTDERSWVYSPLHYSARPASDGESDTVSPQ

Target ORF information:

RefSeq Version	XM_017594818.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphatidylinositol-4-phosphate 5-kinase type 1 gamma (Pip5k1c), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594818.1

ORF Insert Sequence:

ATGACCCAGA AGAAGGCTAT CCTTGCAGAG GCCCCGTTGG TGACAGGGCA GCCAGGCCCT 
GGCCATGGGA AGAAGCTGGG CCATCGAGGC GTGGATGCGT CTGGAGAGAC TACGTATAAG 
AAGACCACCT CGTCCACCCT GAAGGGTGCC ATCCAACTGG GGATTGGGTA CACGGTGGGC 
AACCTCAGCT CTAAGCCAGA ACGCGATGTG CTCATGCAGG ACTTCTATGT GGTGGAGAGC 
ATCTTCTTTC CCAGCGAAGG GAGCAACCTT ACCCCCGCCC ACCACTTCCA GGATTTCCGT 
TTCAAGACCT ATGCACCTGT TGCCTTCCGT TACTTCCGGG AGCTCTTTGG TATCCGTCCG 
GACGATTACT TGTACTCACT CTGCAACGAG CCACTCATTG AGCTGTCCAA CCCGGGTGCC 
AGTGGCTCCG TCTTCTACGT CACCAGCGAT GACGAGTTCA TCATTAAGAC TGTCATGCAC 
AAGGAGGCGG AGTTCCTGCA GAAACTGCTG CCTGGCTACT ATATGAACCT CAACCAGAAC 
CCCCGGACCT TGCTGCCCAA GTTCTATGGG CTGTACTGTG TGCAGTCCGG CGGCAAGAAC 
ATTCGAGTGG TGGTCATGAA CAATGTGCTG CCCCGTGTCG TCAAGATGCA CCTCAAGTTC 
GACCTCAAGG GCTCCACGTA CAAGCGCAGG GCCAGCAAGA AGGAGAAGGA GAAGAGCCTG 
CCCACCTACA AGGACCTGGA CTTCATGCAG GACATGCCAG AGGGGCTGCT ACTGGACTCT 
GACACCTTTG GCGCCCTGGT CAAGACGCTG CAGCGAGACT GCCTGGTGTT GGAGAGCTTC 
AAGATAATGG ACTACAGCCT GCTGCTGGGT GTGCACAACA TTGACCAGCA GGAGCGGGAG 
CGCCAGGCCG AGGGCGCCCA GAGCAAGGCT GACGAGAAGC GGCCGGTGGC CCAGAAGGCC 
CTCTACTCCA CAGCCATGGA GTCCATTCAG GGCGGAGCTG CCCGTGGGGA GGCCATTGAG 
ACAGATGACA CGATGGGTGG GATCCCAGCC GTGAATGGGC GTGGGGAGCG CTTGCTCCTA 
CACATCGGAA TCATTGACAT TCTGCAGTCC TACAGGTTCA TCAAGAAGTT AGAACACACC 
TGGAAGGCCC TCGTCCACGA TGGGGATACC GTCTCAGTCC ACCGGCCCAG CTTCTATGCC 
GAGCGCTTCT TCAAGTTCAT GAGCAGCACG GTCTTCCGGA AGAGTTCCTC CCTCAAGTCC 
TCTCCATCTA AGAAAGGGCG TGGCGCCCTG CTCGCAGTCA AGCCCCTGGG GCCCACCGCA 
GCCTTCTCAG CTAGCCAGAT CCCCAGTGAG AGAGAGGATG TGCAGTACGA CCTGCGGGGA 
GCCCGCAGCT ACCCCACGCT GGAGGATGAA GGCCGGCCTG ACCTCCTGCC CTGCACCCCA 
CCGTCCTTTG AGGAAGCCAC CACAGCCTCT ATCGCCACCA CCCTGTCATC CACCTCCCTC 
TCCATCCCAG AGCGGTCCCC TTCAGACACA TCAGAGCAGC CCCGGTACAG GCGGCGTACG 
CAGTCTTCAG GCCAGGATGG CCGGCCCCAG GAGGAGCTGC ATGCGGAAGA CCTGCAGAAG 
ATAACTGTGC AGGTGGAGCC AGTGTGTGGT GTGGGGGTTG TCGTCCCCAA GGAGCAGGGT 
GCAGGAGTGG AGGTTCCCCC ATCTGGGGCA TCGGCAGCAG CCACGGTGGA AGTAGACGCT 
GCCAGCCAGG CCTCAGAGCC CGCCAGCCAA GCCTCTGATG AGGAAGATGC GCCTTCCACA 
GACATCTATT TTTTCGCCCA CGGGAGATAC TGGCTTTTCT CTCCCCGTCG TCGCCAACTG 
CGGGCCGTGA CACCAAGCCA CACAGGCGCT CCCACCGACG AGAGGAGCTG GGTGTACTCC 
CCGCTTCACT ATAGCGCACG GCCCGCCTCC GACGGCGAGA GCGACACAGT AAGTCCCCAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

LAPTM4B is a PtdIns(4,5)P2 effector that regulates EGFR signaling, lysosomal sorting, and degradation.
The EMBO journal34(4)475-90(2015 Feb)
Tan X,Sun Y,Thapa N,Liao Y,Hedman AC,Anderson RA

Platelets lacking PIP5KI? have normal integrin activation but impaired cytoskeletal-membrane integrity and adhesion.
Blood121(14)2743-52(2013 Apr)
Wang Y,Zhao L,Suzuki A,Lian L,Min SH,Wang Z,Litvinov RI,Stalker TJ,Yago T,Klopocki AG,Schmidtke DW,Yin H,Choi JK,McEver RP,Weisel JW,Hartwig JH,Abrams CS

Synaptic vesicle endocytosis.
Cold Spring Harbor perspectives in biology4(9)a005645(2012 Sep)
Saheki Y,De Camilli P

NMDA receptor-mediated PIP5K activation to produce PI(4,5)P? is essential for AMPA receptor endocytosis during LTD.
Neuron73(1)135-48(2012 Jan)
Unoki T,Matsuda S,Kakegawa W,Van NT,Kohda K,Suzuki A,Funakoshi Y,Hasegawa H,Yuzaki M,Kanaho Y

Phosphatidylinositol-4-phosphate 5-kinases and phosphatidylinositol 4,5-bisphosphate synthesis in the brain.
The Journal of biological chemistry285(37)28708-14(2010 Sep)
Volpicelli-Daley LA,Lucast L,Gong LW,Liu L,Sasaki J,Sasaki T,Abrams CS,Kanaho Y,De Camilli P

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Pip5k1c cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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