Madcam1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Madcam1
Entrez Gene ID	54266
Full Name	mucosal vascular addressin cell adhesion molecule 1
General protein information	Preferred Names mucosal vascular addressin cell adhesion molecule 1 Names mucosal addressin cell adhesion molecule 1 MAdCAM-1 rMAdCAM-1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q11
Summary	mediates the rolling and sticking of lymphocytes to the high endothelial venule (HEV) of the Peyers patch [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_019317.1	NP_062190.1	mucosal addressin cell adhesion molecule 1 precursor
XM_008765179.2	XP_008763401.1	mucosal addressin cell adhesion molecule 1 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MADCAM1	NP_001019810.1
Mus musculus (house mouse)	Madcam1	NP_038619.2
Bos taurus (cattle)	MADCAM1	NP_001032910.1
Gallus gallus (chicken)	MADCAM1	XP_003642914.1
Rattus norvegicus (Norway rat)	Madcam1	NP_062190.1

Gene Ontology
Bibliography

Related articles in PubMed

Disruption of disulfide restriction at integrin knees induces activation and ligand-independent signaling of ????.
Zhang K, Pan Y, Qi J, Yue J, Zhang M, Xu C, Li G, Chen J
Journal of cell science126(Pt 21)5030-41(2013 Nov)

CCL25 and CCL28 promote alpha4 beta7-integrin-dependent adhesion of lymphocytes to MAdCAM-1 under shear flow.
Miles A, Liaskou E, Eksteen B, Lalor PF, Adams DH
American journal of physiology. Gastrointestinal and liver physiology294(5)G1257-67(2008 May)

Aberrant endometrial features of pregnancy in diabetic NOD mice.
Burke SD, Dong H, Hazan AD, Croy BA
Diabetes56(12)2919-26(2007 Dec)

Effect of FTY720 in rat small bowel transplantation: expression of mucosal addressin cell adhesion molecule-1.
Sugito K, Koshinaga T, Inoue M, Ikeda T, Hagiwara N, Fukuzawa M
Transplantation proceedings37(10)4472-4(2005 Dec)

Functional involvement of P-selectin and MAdCAM-1 in the recruitment of alpha4beta7-integrin-expressing monocyte-like cells to the pregnant mouse uterus.
Fernekorn U, Butcher EC, Behrends J, Hartz S, Kruse A
European journal of immunology34(12)3423-33(2004 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

During small bowel allograft rejection., FTY720 was found to prevent the down-regulation of MAdCAM-1 expression in endothelial venules
Title: Effect of FTY720 in rat small bowel transplantation: expression of mucosal addressin cell adhesion molecule-1.

Our model of chronic food allergy revealed that lymphocyte migration was increased with MAdCAM-1 upregulation.
Title: Chronic allergy to dietary ovalbumin induces lymphocyte migration to rat small intestinal mucosa that is inhibited by MAdCAM-1.

Treatment with an antibody to this antigen reduces glomerular and tubulointerstitial scarring in a rat model of crescentic glomerulonephritis
Title: Treatment with an antibody to VLA-1 integrin reduces glomerular and tubulointerstitial scarring in a rat model of crescentic glomerulonephritis.

Immunohistochemical analysis of MAdCAM-1 expression during acute rejection on small bowel grafts in rats
Title: Immunohistochemical analysis of MAdCAM-1 expression during acute rejection on small bowel grafts in rats.

The following Madcam1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Madcam1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04399	NM_019317.1 Latest version!	Rattus norvegicus mucosal vascular addressin cell adhesion molecule 1 (Madcam1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
ORa43413	XM_008765179.2 Latest version!	Rattus norvegicus mucosal vascular addressin cell adhesion molecule 1 (Madcam1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Madcam1 ( NM_019317.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062190.1 Rattus norvegicus mucosal vascular addressin cell adhesion molecule 1 (Madcam1), mRNA.

Price & Availability↑

CloneID	ORa04399
Clone ID Related Accession (Same CDS sequence)	NM_019317.1
Accession Version	NM_019317.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Rattus norvegicus(Norway rat)
Product	mucosal addressin cell adhesion molecule 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D87840.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D87840.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906348 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCCCA TCCTGGCCCT CCTGCTGGCC CTGGGACCCT TCCAGCTCAG CAGAGGCCAG 
TCCTTCCAGG TGAATCCTCC TGAGCCTGAG GTAGCTGTAG CCATGGGCAC ATCCCTCCAG 
ATCAACTGCA GCATGTCCTG TGACAAGGAT ATAGCCCGGG TGCACTGGCA TGGCCTGGAC 
ACCAACCTGG GCAATGTGCA GACCCTCCCA GGCAGCAGGG TCCTCTCCGT ACGAGGCATG 
CTGTCAGACA CGGGCACTCG TGTATGCGTG GGTTCCTGTG GGAGTCGAAG CTTTCAGCAC 
TCTGTGAAGA TCCTTGTGTA TGCCTTTCCA GACCAGCTGG AGGTAACCCC GGAGTTCCTT 
GTACCTGGAC GGGACCAGGT AGTGTCCTGC ACAGCCCACA ACATCTGGCC TGCAGGCCCG 
GACAGTCTTT CCTTTGCCTT GCTCCGAGGA GAGCAGAGCC TGGAGGGTGC TCAGGCCCTG 
GAAACAGAGC AAGAGGAGGA GATGCAAGAG ACTGAGGGCA CTCCACTCTT CCAAGTGACA 
CAACGCTGGT TGCTGCCCTC CCTGGGGACC CCCGCCCTTC CCGCCCTTTA TTGCCAGGTC 
ACCATGCAGC TGCCCAAACT GGTGCTGACA CATAGAAGGA AGATTCCAGT CCTACAGAGC 
CAGACCTCAC CAGAGCCCCC CAGCACCACC TCTGCTAAGC CATACATCCT GACCTCATCA 
CATACTACTA AGGCAGTCTC CACTGGGCTC AGCAGTGTAG CCCTGCCTTC TACGCCTCTG 
AGCTCCGAGG GGCCCTGCTA CCCCGAAATC CACCAGAACC CAGAGGCAGA CTGGGAACTT 
CTCTGCGAAG CCTCCTGTGG GTCTGGAGTG ACGGTGCATT GGACCCTGGC TCCTGGTGAC 
CTGGCAGCCT ACCACAAGAG AGAAGCTGGG GCCCAGGCAT GGCTAAGCGT GCTGCCCCTG 
GGCCCCATTC CTGAGGGCTG GTTCCAGTGT CGCATGGACC CTGGCGGGCA GGTGACCAGT 
CTGTATGTTA CTGGCCAGGT GATCCCAAAC CCCTCCTCCA TGGTCGCCCT GTGGATTGGC 
AGCTTGGTGC TGGGGCTGCT TGCACTCGCC TTCCTTGCCT ACTGCCTGTG GAAACGCTAC 
CGGCCGGGTC CTCTCCCAGA CTCCAGCTCG TGTACGCTCC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062190.1
CDS	14..1198
Translation	MEPILALLLALGPFQLSRGQSFQVNPPEPEVAVAMGTSLQINCSMSCDKDIARVHWHGLDTNLGNVQTLPGSRVLSVRGMLSDTGTRVCVGSCGSRSFQHSVKILVYAFPDQLEVTPEFLVPGRDQVVSCTAHNIWPAGPDSLSFALLRGEQSLEGAQALETEQEEEMQETEGTPLFQVTQRWLLPSLGTPALPALYCQVTMQLPKLVLTHRRKIPVLQSQTSPEPPSTTSAKPYILTSSHTTKAVSTGLSSVALPSTPLSSEGPCYPEIHQNPEADWELLCEASCGSGVTVHWTLAPGDLAAYHKREAGAQAWLSVLPLGPIPEGWFQCRMDPGGQVTSLYVTGQVIPNPSSMVALWIGSLVLGLLALAFLAYCLWKRYRPGPLPDSSSCTLL

Target ORF information:

RefSeq Version	NM_019317.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mucosal vascular addressin cell adhesion molecule 1 (Madcam1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019317.1

ORF Insert Sequence:

ATGGAGCCCA TCCTGGCCCT CCTGCTGGCC CTGGGACCCT TCCAGCTCAG CAGAGGCCAG 
TCCTTCCAGG TGAATCCTCC TGAGCCTGAG GTAGCTGTAG CCATGGGCAC ATCCCTCCAG 
ATCAACTGCA GCATGTCCTG TGACAAGGAT ATAGCCCGGG TGCACTGGCA TGGCCTGGAC 
ACCAACCTGG GCAATGTGCA GACCCTCCCA GGCAGCAGGG TCCTCTCCGT ACGAGGCATG 
CTGTCAGACA CGGGCACTCG TGTATGCGTG GGTTCCTGTG GGAGTCGAAG CTTTCAGCAC 
TCTGTGAAGA TCCTTGTGTA TGCCTTTCCA GACCAGCTGG AGGTAACCCC GGAGTTCCTT 
GTACCTGGAC GGGACCAGGT AGTGTCCTGC ACAGCCCACA ACATCTGGCC TGCAGGCCCG 
GACAGTCTTT CCTTTGCCTT GCTCCGAGGA GAGCAGAGCC TGGAGGGTGC TCAGGCCCTG 
GAAACAGAGC AAGAGGAGGA GATGCAAGAG ACTGAGGGCA CTCCACTCTT CCAAGTGACA 
CAACGCTGGT TGCTGCCCTC CCTGGGGACC CCCGCCCTTC CCGCCCTTTA TTGCCAGGTC 
ACCATGCAGC TGCCCAAACT GGTGCTGACA CATAGAAGGA AGATTCCAGT CCTACAGAGC 
CAGACCTCAC CAGAGCCCCC CAGCACCACC TCTGCTAAGC CATACATCCT GACCTCATCA 
CATACTACTA AGGCAGTCTC CACTGGGCTC AGCAGTGTAG CCCTGCCTTC TACGCCTCTG 
AGCTCCGAGG GGCCCTGCTA CCCCGAAATC CACCAGAACC CAGAGGCAGA CTGGGAACTT 
CTCTGCGAAG CCTCCTGTGG GTCTGGAGTG ACGGTGCATT GGACCCTGGC TCCTGGTGAC 
CTGGCAGCCT ACCACAAGAG AGAAGCTGGG GCCCAGGCAT GGCTAAGCGT GCTGCCCCTG 
GGCCCCATTC CTGAGGGCTG GTTCCAGTGT CGCATGGACC CTGGCGGGCA GGTGACCAGT 
CTGTATGTTA CTGGCCAGGT GATCCCAAAC CCCTCCTCCA TGGTCGCCCT GTGGATTGGC 
AGCTTGGTGC TGGGGCTGCT TGCACTCGCC TTCCTTGCCT ACTGCCTGTG GAAACGCTAC 
CGGCCGGGTC CTCTCCCAGA CTCCAGCTCG TGTACGCTCC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madcam1 ( XM_008765179.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008763401.1 Rattus norvegicus mucosal vascular addressin cell adhesion molecule 1 (Madcam1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa43413
Clone ID Related Accession (Same CDS sequence)	XM_008765179.2
Accession Version	XM_008765179.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mucosal addressin cell adhesion molecule 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008765179.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCA TCCTGGCCCT CCTGCTGGCC CTGGGACCCT TCCAGCTCAG CAGAGGCCAG 
TCCTTCCAGG TGAATCCTCC TGAGCCTGAG GTAGCTGTAG CCATGGGCAC ATCCCTCCAG 
ATCAACTGCA GCATGTCCTG TGACAAGGAT ATAGCCCGGG TGCACTGGCA TGGCCTGGAC 
ACCAACCTGG GCAATGTGCA GACCCTCCCA GGCAGCAGGG TCCTCTCCGT ACGAGGCATG 
CTGTCAGACA CGGGCACTCG TGTATGCGTG GGTTCCTGTG GGAGTCGAAG CTTTCAGCAC 
TCTGTGAAGA TCCTTGTGTA TGCCTTTCCA GACCAGCTGG AGGTAACCCC GGAGTTCCTT 
GTACCTGGAC GGGACCAGGT AGTGTCCTGC ACAGCCCACA ACATCTGGCC TGCAGGCCCG 
GACAGTCTTT CCTTTGCCTT GCTCCGAGGA GAGCAGAGCC TGGAGGGTGC TCAGGCCCTG 
GAAACAGAGC AAGAGGAGGA GATGCAAGAG ACTGAGGGCA CTCCACTCTT CCAAGTGACA 
CAACGCTGGT TGCTGCCCTC CCTGGGGACC CCCGCCCTTC CCGCCCTTTA TTGCCAGGTC 
ACCATGCAGC TGCCCAAACT GGTGCTGACA CATAGAAGGA AGATTCCAGT CCTACAGAGC 
CAGACCTCAC CAGAGCCCCC CAGCACCACC TCTGCTAAGC CATACATCCT GACCTCATCA 
CATACTACTA AGGCAGTCTC CACTGGGCTC AGCAGTGTAG CCCTGCCTTC TACGCCTCTG 
AGCTCCGAGG GGCCCTGCTA CCCCGAAATC CACCAGAACC CAGAGGCAGA CTGGGAACTT 
CTCTGCGAAG CCTCCTGTGG GTCTGGAGTG ACGGTGCATT GGACCCTGGC TCCTGGTGAC 
CTGGCAGCCT ACCACAAGAG AGAAGCTGGG GCCCAGGCAT GGCTAAGCGT GCTGCCCCTG 
GGCCCCATTC CTGAGGGCTG GTTCCAGTGT CGCATGGACC CTGGCGGGCA GCCTCCATGG 
TCGCCCTGTG GATTGGCAGC TTGGTGCTGG GGCTGCTTGC ACTCGCCTTC CTTGCCTACT 
GCCTGTGGAA ACGCTACCGG CCGGGTCCTC TCCCAGACTC CAGCTCGTGT ACGCTCCTAT 
GAAGTTCCAT TATGCCAGAC TAAGGGAGGC AAAGGATTAC CAGATGTAGG TTTGGGGCAT 
CAAGATGATA GTGTGGCCCC TTTCCTTGGC GGTCAGCACA TCGACAAGTT CCTCCCAACT 
ACTGGACTTT CCTGCCCGCT GGCCCAGAGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008763401.1
CDS	411..1703
Translation	MEPILALLLALGPFQLSRGQSFQVNPPEPEVAVAMGTSLQINCSMSCDKDIARVHWHGLDTNLGNVQTLPGSRVLSVRGMLSDTGTRVCVGSCGSRSFQHSVKILVYAFPDQLEVTPEFLVPGRDQVVSCTAHNIWPAGPDSLSFALLRGEQSLEGAQALETEQEEEMQETEGTPLFQVTQRWLLPSLGTPALPALYCQVTMQLPKLVLTHRRKIPVLQSQTSPEPPSTTSAKPYILTSSHTTKAVSTGLSSVALPSTPLSSEGPCYPEIHQNPEADWELLCEASCGSGVTVHWTLAPGDLAAYHKREAGAQAWLSVLPLGPIPEGWFQCRMDPGGQPPWSPCGLAAWCWGCLHSPSLPTACGNATGRVLSQTPARVRSYEVPLCQTKGGKGLPDVGLGHQDDSVAPFLGGQHIDKFLPTTGLSCPLAQS

Target ORF information:

RefSeq Version	XM_008765179.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mucosal vascular addressin cell adhesion molecule 1 (Madcam1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765179.2

ORF Insert Sequence:

ATGGAGCCCA TCCTGGCCCT CCTGCTGGCC CTGGGACCCT TCCAGCTCAG CAGAGGCCAG 
TCCTTCCAGG TGAATCCTCC TGAGCCTGAG GTAGCTGTAG CCATGGGCAC ATCCCTCCAG 
ATCAACTGCA GCATGTCCTG TGACAAGGAT ATAGCCCGGG TGCACTGGCA TGGCCTGGAC 
ACCAACCTGG GCAATGTGCA GACCCTCCCA GGCAGCAGGG TCCTCTCCGT ACGAGGCATG 
CTGTCAGACA CGGGCACTCG TGTATGCGTG GGTTCCTGTG GGAGTCGAAG CTTTCAGCAC 
TCTGTGAAGA TCCTTGTGTA TGCCTTTCCA GACCAGCTGG AGGTAACCCC GGAGTTCCTT 
GTACCTGGAC GGGACCAGGT AGTGTCCTGC ACAGCCCACA ACATCTGGCC TGCAGGCCCG 
GACAGTCTTT CCTTTGCCTT GCTCCGAGGA GAGCAGAGCC TGGAGGGTGC TCAGGCCCTG 
GAAACAGAGC AAGAGGAGGA GATGCAAGAG ACTGAGGGCA CTCCACTCTT CCAAGTGACA 
CAACGCTGGT TGCTGCCCTC CCTGGGGACC CCCGCCCTTC CCGCCCTTTA TTGCCAGGTC 
ACCATGCAGC TGCCCAAACT GGTGCTGACA CATAGAAGGA AGATTCCAGT CCTACAGAGC 
CAGACCTCAC CAGAGCCCCC CAGCACCACC TCTGCTAAGC CATACATCCT GACCTCATCA 
CATACTACTA AGGCAGTCTC CACTGGGCTC AGCAGTGTAG CCCTGCCTTC TACGCCTCTG 
AGCTCCGAGG GGCCCTGCTA CCCCGAAATC CACCAGAACC CAGAGGCAGA CTGGGAACTT 
CTCTGCGAAG CCTCCTGTGG GTCTGGAGTG ACGGTGCATT GGACCCTGGC TCCTGGTGAC 
CTGGCAGCCT ACCACAAGAG AGAAGCTGGG GCCCAGGCAT GGCTAAGCGT GCTGCCCCTG 
GGCCCCATTC CTGAGGGCTG GTTCCAGTGT CGCATGGACC CTGGCGGGCA GCCTCCATGG 
TCGCCCTGTG GATTGGCAGC TTGGTGCTGG GGCTGCTTGC ACTCGCCTTC CTTGCCTACT 
GCCTGTGGAA ACGCTACCGG CCGGGTCCTC TCCCAGACTC CAGCTCGTGT ACGCTCCTAT 
GAAGTTCCAT TATGCCAGAC TAAGGGAGGC AAAGGATTAC CAGATGTAGG TTTGGGGCAT 
CAAGATGATA GTGTGGCCCC TTTCCTTGGC GGTCAGCACA TCGACAAGTT CCTCCCAACT 
ACTGGACTTT CCTGCCCGCT GGCCCAGAGC TAA