fgd3 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	fgd3
Entrez Gene ID	100490625
Full Name	FYVE, RhoGEF and PH domain containing 3
Synonyms	zfyve5
General protein information	Preferred Names FYVE, RhoGEF and PH domain containing 3 Names FYVE, RhoGEF and PH domain-containing protein 3
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002941737.5	XP_002941783.3	FYVE, RhoGEF and PH domain-containing protein 3 isoform X2
XM_012961791.3	XP_012817245.2	FYVE, RhoGEF and PH domain-containing protein 3 isoform X2
XM_012961791.2	XP_012817245.1	FYVE, RhoGEF and PH domain-containing protein 3 isoform X2
XM_004914180.3	XP_004914237.1	FYVE, RhoGEF and PH domain-containing protein 3 isoform X1
XM_002941737.4	XP_002941783.2	FYVE, RhoGEF and PH domain-containing protein 3 isoform X2
XM_004914180.4	XP_004914237.2	FYVE, RhoGEF and PH domain-containing protein 3 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	FGD3	XP_005616048.1
Bos taurus (cattle)	FGD3	NP_001019698.1
Danio rerio (zebrafish)	fgd	NP_571631.2
Pan troglodytes (chimpanzee)	FGD3	XP_003312224.1
Mus musculus (house mouse)	Fgd3	NP_056574.2
Rattus norvegicus (Norway rat)	Fgd3	NP_001101879.1
Xenopus tropicalis (tropical clawed frog)	fgd3	XP_004914237.1
Homo sapiens (human)	FGD3	NP_001077005.1
Gallus gallus (chicken)	FGD3	XP_414331.4
	FGD3	XP_001104111.1

Gene Ontology

The following fgd3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fgd3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa09805	XM_004914180.3 Latest version!	Xenopus tropicalis FYVE, RhoGEF and PH domain containing 3 (fgd3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	Quote Price
OXa09806	XM_012961791.2 Latest version!	Xenopus tropicalis FYVE, RhoGEF and PH domain containing 3 (fgd3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	Quote Price
OXa09806	XM_002941737.4 Latest version!	Xenopus tropicalis FYVE, RhoGEF and PH domain containing 3 (fgd3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	Quote Price
OXa57664	XM_004914180.4 Latest version!	Xenopus tropicalis FYVE, RhoGEF and PH domain containing 3 (fgd3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	Quote Price
OXa57665	XM_012961791.3 Latest version!	Xenopus tropicalis FYVE, RhoGEF and PH domain containing 3 (fgd3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	Quote Price
OXa57665	XM_002941737.5 Latest version!	Xenopus tropicalis FYVE, RhoGEF and PH domain containing 3 (fgd3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	Quote Price

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

fgd3 ( XM_004914180.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914237.1 Xenopus tropicalis FYVE, RhoGEF and PH domain containing 3 (fgd3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa09805
Clone ID Related Accession (Same CDS sequence)	XM_004914180.3
Accession Version	XM_004914180.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2691bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	FYVE, RhoGEF and PH domain-containing protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683495.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004914180.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAGAA TGACTGGCCC CAGGGCAGAG GGTCGCAGCT GCGCTCCCTC CCAGCTCAGA 
TGTCACATTG AATCGAGTGC GTTCGGGCGG CCGCAGCAAG TTGGCCCCGT ACCATGTATC 
CAGCAAAGCG CAAGGAAGTG GAAGTGCAAA CCTCGGCCAA TCAGAACACT TCCCTCCGAA 
AAACCCCAAG TGCCCCCTAA ACCCGCCCAT CTGAAAGCAT CGGGGAAAGA AATACTAAAC 
CACCGAATTC CTCCTGCCCC CTCAAAGCCA TTGCCCCTGG TACCCAAACC CCCCCGCCGG 
CCTGGGGTTA AAGAGCGGGG CAAGTCTGGA ACCTCCGACA TCAACCTCCT CATCAAGAAG 
TTTGAGAGCC TCAGGCAGCC CGTCCAGTTT GGGAGACACA GTCGGTACCA TTTTAGTGCC 
AGGATGGAGC AAGGGACGAG CCCCCTGTGT TGCCCCCATG TTTCTGCGAG GGACTCGGGG 
TCGGAGAGTG GGGTCCCTTT GGGGGAGCCG GGCAGGACGG CGGAGGAGGA ACCCACTGCA 
GAGCTGCCCA TGGATAATCT CAAAATGAGA GACCTTACAA TAAACGATAT TAACAACCGC 
GGGGGTAACA ACGCCTCTGT GAACAGGACT GAGCGCCCCC CGCAAGAGAA CGGAAACCTG 
ACCGTCGATC ACAACGACAA TGGGAAAATT CCCAACAGGG ACAGCGGCAT CGACAGCCCT 
TCCTGCAGCG TGGTCGGGGA GGGGTTCTCC AACGACGAGA GCGCCGAGCC CAAAAAGCTC 
TCCCTGCCGG TCCACATGGT GGAAATTGGG ATTGTCCGGA AGAACTCGGA CTCGTCGTCT 
GTGGATCAGA AACGGGACTC GACCCAAGAT GAGGACAGCG ACCTGGACGA GGGCAGCAGC 
GAAGAACATG AAGCCATGGA GGTGCCCAAG GCAGAGAGCG TCGATCCCAC CAAATGTTCT 
GAGCAGCAGA GACTGTACAA CATCGCCAGA GAGCTGCTAC AGACGGAGGA GGCGTATGTG 
AAGAGGCTGG ATCTGCTGGA TCAGGTGTTC TGCACAAGGT TGTTATCTGC CGGGGTCCCC 
CAGGAGGTGA TCAATGGAAT ATTCTCCAAT ATATCCTCCA TCTATCGCTT TCACTATCAG 
TTCCTCCTTC CCGAACTAAA GGCGAGAATA ATGCAAGAGT GGGATACGAA CCCGCGGATC 
GGGGATATCC TACAGAAACT CGCCCCGTTC CTGAAGATGT ACGGGGAATA CGTCAAGAAC 
TTCGATAAAG CGATGGATAT GGTCAACAGC TGGACCCAGC GCTCTTCTAC CTTTAAGGAG 
GTAGTTCATA CAATACAGAA GGACCCGGTG TGCGGGAACC TGACCCTGCA GCACCACATG 
TTGGAGCCGG TGCAGAGGAT TCCCCGCTAC GAGCTGCTCC TGAAGGATTA TCTGAAGAAA 
CTCCCAGACG ACTCTCCGGA TCGCAAGGAT TCAGAAAAGT CCCTGGAGTT AATTTCTACT 
GCGGCCAATC ACTCAAACGC AGCCATCCGC AAAATGGAGA AGATGCACAA ACTGCTGGAG 
GTGTATGAGC GACTCGGGGG GGAGGAGGAT ATAGTTAATC CGGCCAATGA GCTCATTAAG 
GAGGGGCACA TACAGAAACT GTCGGCGAAG AACGGGACCA CGCAGGACCG GTACCTGTAC 
CTGTTTAACA GCATGCTCCT GTACTGCGTC CCCAAGCTGC GCCTCATGGG CCAGAAGTTC 
AGCGTCCGGG AGAAGATCGA CATCGCCGGG ATCCAGGTAC AGGAGACCGT GAAGAACAAT 
TCCCCTCATA TGTTCCAGAT AATCGGCAAA AAGAGATCCC TGGAGCTGCA GGCGAGGACA 
GAGGAGGAGA AGAAGGAATG GATACAGGTC ATCCAAGCCA CGATCGAGAA GCACAAACAG 
AACAGCGAGA CCTTCCGGGC GTTTAACAGT TCCTTTTCCA GAGACGATGA GCATTGCCCC 
GATTCCCCGG TGCCGGGCAC GGGCCCCCCG GATTATTCCT CAGGGATGGA CGGCTGCAGC 
TCTCCTGGAG GGGCCGATTC CTTCCGGAAA TCCTGTAAGA TAAAGCGGGA GAAGGAGAAG 
CAAAACTGCA AGGGCTGCGG GGAGAGTTTC AACTCCATAA CGAAGCGCAG GCACCACTGC 
AAGCAATGCG GCGCCGTGAT TTGCGCAAAG TGCTCGGAGT TCAAGCTGCT GGCGGAGAAC 
AGTCGGCATA ACCGGGTCTG TAAGGAGTGT TTCCCTCTCG TCTCCTCTGT CCCGGGGAGT 
CCGGGGGGCC CCACAGAGTC GGGGGACACA AAGAAGAAAG TGGAGAAGCA GAATTCCTTG 
TCTCCGGAAA ACTGTGTGTT CAGCAGTAGC CTCCTGTTCC TGGAGAAGGG GAGAACGTGG 
ACGAAAGTGT GGGTGTCGGT GCCCAGGAAC GAGCCCCTCG TCATGTATCT GCAGGGGGGC 
AGCCAGGATG GGCGGCCCCC CCGGGCAATA CCGTTGCCAG GATACGAAGT GGGTTTGCCA 
AGCGCCGGTG ACAAAGTGGA CTTGAAACAC GTATTTAAAC TGTCGCAGTC GCAGCAAGTT 
CTGTTCTTCG GGGCGGAGGA TGAGGATTTG CTGCAGAAGT GGAAGGGGAT TCTCAGTAAA 
GCCTCTCGGG GGGACCCCCC CATTGTGGCA GAGGATCCAG AAAAGCTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914237.1
CDS	183..2873
Translation	MNRMTGPRAEGRSCAPSQLRCHIESSAFGRPQQVGPVPCIQQSARKWKCKPRPIRTLPSEKPQVPPKPAHLKASGKEILNHRIPPAPSKPLPLVPKPPRRPGVKERGKSGTSDINLLIKKFESLRQPVQFGRHSRYHFSARMEQGTSPLCCPHVSARDSGSESGVPLGEPGRTAEEEPTAELPMDNLKMRDLTINDINNRGGNNASVNRTERPPQENGNLTVDHNDNGKIPNRDSGIDSPSCSVVGEGFSNDESAEPKKLSLPVHMVEIGIVRKNSDSSSVDQKRDSTQDEDSDLDEGSSEEHEAMEVPKAESVDPTKCSEQQRLYNIARELLQTEEAYVKRLDLLDQVFCTRLLSAGVPQEVINGIFSNISSIYRFHYQFLLPELKARIMQEWDTNPRIGDILQKLAPFLKMYGEYVKNFDKAMDMVNSWTQRSSTFKEVVHTIQKDPVCGNLTLQHHMLEPVQRIPRYELLLKDYLKKLPDDSPDRKDSEKSLELISTAANHSNAAIRKMEKMHKLLEVYERLGGEEDIVNPANELIKEGHIQKLSAKNGTTQDRYLYLFNSMLLYCVPKLRLMGQKFSVREKIDIAGIQVQETVKNNSPHMFQIIGKKRSLELQARTEEEKKEWIQVIQATIEKHKQNSETFRAFNSSFSRDDEHCPDSPVPGTGPPDYSSGMDGCSSPGGADSFRKSCKIKREKEKQNCKGCGESFNSITKRRHHCKQCGAVICAKCSEFKLLAENSRHNRVCKECFPLVSSVPGSPGGPTESGDTKKKVEKQNSLSPENCVFSSSLLFLEKGRTWTKVWVSVPRNEPLVMYLQGGSQDGRPPRAIPLPGYEVGLPSAGDKVDLKHVFKLSQSQQVLFFGAEDEDLLQKWKGILSKASRGDPPIVAEDPEKL

Target ORF information:

RefSeq Version	XM_004914180.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis FYVE, RhoGEF and PH domain containing 3 (fgd3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914180.3

ORF Insert Sequence:

ATGAACAGAA TGACTGGCCC CAGGGCAGAG GGTCGCAGCT GCGCTCCCTC CCAGCTCAGA 
TGTCACATTG AATCGAGTGC GTTCGGGCGG CCGCAGCAAG TTGGCCCCGT ACCATGTATC 
CAGCAAAGCG CAAGGAAGTG GAAGTGCAAA CCTCGGCCAA TCAGAACACT TCCCTCCGAA 
AAACCCCAAG TGCCCCCTAA ACCCGCCCAT CTGAAAGCAT CGGGGAAAGA AATACTAAAC 
CACCGAATTC CTCCTGCCCC CTCAAAGCCA TTGCCCCTGG TACCCAAACC CCCCCGCCGG 
CCTGGGGTTA AAGAGCGGGG CAAGTCTGGA ACCTCCGACA TCAACCTCCT CATCAAGAAG 
TTTGAGAGCC TCAGGCAGCC CGTCCAGTTT GGGAGACACA GTCGGTACCA TTTTAGTGCC 
AGGATGGAGC AAGGGACGAG CCCCCTGTGT TGCCCCCATG TTTCTGCGAG GGACTCGGGG 
TCGGAGAGTG GGGTCCCTTT GGGGGAGCCG GGCAGGACGG CGGAGGAGGA ACCCACTGCA 
GAGCTGCCCA TGGATAATCT CAAAATGAGA GACCTTACAA TAAACGATAT TAACAACCGC 
GGGGGTAACA ACGCCTCTGT GAACAGGACT GAGCGCCCCC CGCAAGAGAA CGGAAACCTG 
ACCGTCGATC ACAACGACAA TGGGAAAATT CCCAACAGGG ACAGCGGCAT CGACAGCCCT 
TCCTGCAGCG TGGTCGGGGA GGGGTTCTCC AACGACGAGA GCGCCGAGCC CAAAAAGCTC 
TCCCTGCCGG TCCACATGGT GGAAATTGGG ATTGTCCGGA AGAACTCGGA CTCGTCGTCT 
GTGGATCAGA AACGGGACTC GACCCAAGAT GAGGACAGCG ACCTGGACGA GGGCAGCAGC 
GAAGAACATG AAGCCATGGA GGTGCCCAAG GCAGAGAGCG TCGATCCCAC CAAATGTTCT 
GAGCAGCAGA GACTGTACAA CATCGCCAGA GAGCTGCTAC AGACGGAGGA GGCGTATGTG 
AAGAGGCTGG ATCTGCTGGA TCAGGTGTTC TGCACAAGGT TGTTATCTGC CGGGGTCCCC 
CAGGAGGTGA TCAATGGAAT ATTCTCCAAT ATATCCTCCA TCTATCGCTT TCACTATCAG 
TTCCTCCTTC CCGAACTAAA GGCGAGAATA ATGCAAGAGT GGGATACGAA CCCGCGGATC 
GGGGATATCC TACAGAAACT CGCCCCGTTC CTGAAGATGT ACGGGGAATA CGTCAAGAAC 
TTCGATAAAG CGATGGATAT GGTCAACAGC TGGACCCAGC GCTCTTCTAC CTTTAAGGAG 
GTAGTTCATA CAATACAGAA GGACCCGGTG TGCGGGAACC TGACCCTGCA GCACCACATG 
TTGGAGCCGG TGCAGAGGAT TCCCCGCTAC GAGCTGCTCC TGAAGGATTA TCTGAAGAAA 
CTCCCAGACG ACTCTCCGGA TCGCAAGGAT TCAGAAAAGT CCCTGGAGTT AATTTCTACT 
GCGGCCAATC ACTCAAACGC AGCCATCCGC AAAATGGAGA AGATGCACAA ACTGCTGGAG 
GTGTATGAGC GACTCGGGGG GGAGGAGGAT ATAGTTAATC CGGCCAATGA GCTCATTAAG 
GAGGGGCACA TACAGAAACT GTCGGCGAAG AACGGGACCA CGCAGGACCG GTACCTGTAC 
CTGTTTAACA GCATGCTCCT GTACTGCGTC CCCAAGCTGC GCCTCATGGG CCAGAAGTTC 
AGCGTCCGGG AGAAGATCGA CATCGCCGGG ATCCAGGTAC AGGAGACCGT GAAGAACAAT 
TCCCCTCATA TGTTCCAGAT AATCGGCAAA AAGAGATCCC TGGAGCTGCA GGCGAGGACA 
GAGGAGGAGA AGAAGGAATG GATACAGGTC ATCCAAGCCA CGATCGAGAA GCACAAACAG 
AACAGCGAGA CCTTCCGGGC GTTTAACAGT TCCTTTTCCA GAGACGATGA GCATTGCCCC 
GATTCCCCGG TGCCGGGCAC GGGCCCCCCG GATTATTCCT CAGGGATGGA CGGCTGCAGC 
TCTCCTGGAG GGGCCGATTC CTTCCGGAAA TCCTGTAAGA TAAAGCGGGA GAAGGAGAAG 
CAAAACTGCA AGGGCTGCGG GGAGAGTTTC AACTCCATAA CGAAGCGCAG GCACCACTGC 
AAGCAATGCG GCGCCGTGAT TTGCGCAAAG TGCTCGGAGT TCAAGCTGCT GGCGGAGAAC 
AGTCGGCATA ACCGGGTCTG TAAGGAGTGT TTCCCTCTCG TCTCCTCTGT CCCGGGGAGT 
CCGGGGGGCC CCACAGAGTC GGGGGACACA AAGAAGAAAG TGGAGAAGCA GAATTCCTTG 
TCTCCGGAAA ACTGTGTGTT CAGCAGTAGC CTCCTGTTCC TGGAGAAGGG GAGAACGTGG 
ACGAAAGTGT GGGTGTCGGT GCCCAGGAAC GAGCCCCTCG TCATGTATCT GCAGGGGGGC 
AGCCAGGATG GGCGGCCCCC CCGGGCAATA CCGTTGCCAG GATACGAAGT GGGTTTGCCA 
AGCGCCGGTG ACAAAGTGGA CTTGAAACAC GTATTTAAAC TGTCGCAGTC GCAGCAAGTT 
CTGTTCTTCG GGGCGGAGGA TGAGGATTTG CTGCAGAAGT GGAAGGGGAT TCTCAGTAAA 
GCCTCTCGGG GGGACCCCCC CATTGTGGCA GAGGATCCAG AAAAGCTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fgd3 ( XM_012961791.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817245.1 Xenopus tropicalis FYVE, RhoGEF and PH domain containing 3 (fgd3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa09806
Clone ID Related Accession (Same CDS sequence)	XM_012961791.2 , XM_002941737.4
Accession Version	XM_012961791.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	FYVE, RhoGEF and PH domain-containing protein 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683495.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961791.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAG GGACGAGCCC CCTGTGTTGC CCCCATGTTT CTGCGAGGGA CTCGGGGTCG 
GAGAGTGGGG TCCCTTTGGG GGAGCCGGGC AGGACGGCGG AGGAGGAACC CACTGCAGAG 
CTGCCCATGG ATAATCTCAA AATGAGAGAC CTTACAATAA ACGATATTAA CAACCGCGGG 
GGTAACAACG CCTCTGTGAA CAGGACTGAG CGCCCCCCGC AAGAGAACGG AAACCTGACC 
GTCGATCACA ACGACAATGG GAAAATTCCC AACAGGGACA GCGGCATCGA CAGCCCTTCC 
TGCAGCGTGG TCGGGGAGGG GTTCTCCAAC GACGAGAGCG CCGAGCCCAA AAAGCTCTCC 
CTGCCGGTCC ACATGGTGGA AATTGGGATT GTCCGGAAGA ACTCGGACTC GTCGTCTGTG 
GATCAGAAAC GGGACTCGAC CCAAGATGAG GACAGCGACC TGGACGAGGG CAGCAGCGAA 
GAACATGAAG CCATGGAGGT GCCCAAGGCA GAGAGCGTCG ATCCCACCAA ATGTTCTGAG 
CAGCAGAGAC TGTACAACAT CGCCAGAGAG CTGCTACAGA CGGAGGAGGC GTATGTGAAG 
AGGCTGGATC TGCTGGATCA GGTGTTCTGC ACAAGGTTGT TATCTGCCGG GGTCCCCCAG 
GAGGTGATCA ATGGAATATT CTCCAATATA TCCTCCATCT ATCGCTTTCA CTATCAGTTC 
CTCCTTCCCG AACTAAAGGC GAGAATAATG CAAGAGTGGG ATACGAACCC GCGGATCGGG 
GATATCCTAC AGAAACTCGC CCCGTTCCTG AAGATGTACG GGGAATACGT CAAGAACTTC 
GATAAAGCGA TGGATATGGT CAACAGCTGG ACCCAGCGCT CTTCTACCTT TAAGGAGGTA 
GTTCATACAA TACAGAAGGA CCCGGTGTGC GGGAACCTGA CCCTGCAGCA CCACATGTTG 
GAGCCGGTGC AGAGGATTCC CCGCTACGAG CTGCTCCTGA AGGATTATCT GAAGAAACTC 
CCAGACGACT CTCCGGATCG CAAGGATTCA GAAAAGTCCC TGGAGTTAAT TTCTACTGCG 
GCCAATCACT CAAACGCAGC CATCCGCAAA ATGGAGAAGA TGCACAAACT GCTGGAGGTG 
TATGAGCGAC TCGGGGGGGA GGAGGATATA GTTAATCCGG CCAATGAGCT CATTAAGGAG 
GGGCACATAC AGAAACTGTC GGCGAAGAAC GGGACCACGC AGGACCGGTA CCTGTACCTG 
TTTAACAGCA TGCTCCTGTA CTGCGTCCCC AAGCTGCGCC TCATGGGCCA GAAGTTCAGC 
GTCCGGGAGA AGATCGACAT CGCCGGGATC CAGGTACAGG AGACCGTGAA GAACAATTCC 
CCTCATATGT TCCAGATAAT CGGCAAAAAG AGATCCCTGG AGCTGCAGGC GAGGACAGAG 
GAGGAGAAGA AGGAATGGAT ACAGGTCATC CAAGCCACGA TCGAGAAGCA CAAACAGAAC 
AGCGAGACCT TCCGGGCGTT TAACAGTTCC TTTTCCAGAG ACGATGAGCA TTGCCCCGAT 
TCCCCGGTGC CGGGCACGGG CCCCCCGGAT TATTCCTCAG GGATGGACGG CTGCAGCTCT 
CCTGGAGGGG CCGATTCCTT CCGGAAATCC TGTAAGATAA AGCGGGAGAA GGAGAAGCAA 
AACTGCAAGG GCTGCGGGGA GAGTTTCAAC TCCATAACGA AGCGCAGGCA CCACTGCAAG 
CAATGCGGCG CCGTGATTTG CGCAAAGTGC TCGGAGTTCA AGCTGCTGGC GGAGAACAGT 
CGGCATAACC GGGTCTGTAA GGAGTGTTTC CCTCTCGTCT CCTCTGTCCC GGGGAGTCCG 
GGGGGCCCCA CAGAGTCGGG GGACACAAAG AAGAAAGTGG AGAAGCAGAA TTCCTTGTCT 
CCGGAAAACT GTGTGTTCAG CAGTAGCCTC CTGTTCCTGG AGAAGGGGAG AACGTGGACG 
AAAGTGTGGG TGTCGGTGCC CAGGAACGAG CCCCTCGTCA TGTATCTGCA GGGGGGCAGC 
CAGGATGGGC GGCCCCCCCG GGCAATACCG TTGCCAGGAT ACGAAGTGGG TTTGCCAAGC 
GCCGGTGACA AAGTGGACTT GAAACACGTA TTTAAACTGT CGCAGTCGCA GCAAGTTCTG 
TTCTTCGGGG CGGAGGATGA GGATTTGCTG CAGAAGTGGA AGGGGATTCT CAGTAAAGCC 
TCTCGGGGGG ACCCCCCCAT TGTGGCAGAG GATCCAGAAA AGCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817245.1
CDS	523..2790
Translation	MEQGTSPLCCPHVSARDSGSESGVPLGEPGRTAEEEPTAELPMDNLKMRDLTINDINNRGGNNASVNRTERPPQENGNLTVDHNDNGKIPNRDSGIDSPSCSVVGEGFSNDESAEPKKLSLPVHMVEIGIVRKNSDSSSVDQKRDSTQDEDSDLDEGSSEEHEAMEVPKAESVDPTKCSEQQRLYNIARELLQTEEAYVKRLDLLDQVFCTRLLSAGVPQEVINGIFSNISSIYRFHYQFLLPELKARIMQEWDTNPRIGDILQKLAPFLKMYGEYVKNFDKAMDMVNSWTQRSSTFKEVVHTIQKDPVCGNLTLQHHMLEPVQRIPRYELLLKDYLKKLPDDSPDRKDSEKSLELISTAANHSNAAIRKMEKMHKLLEVYERLGGEEDIVNPANELIKEGHIQKLSAKNGTTQDRYLYLFNSMLLYCVPKLRLMGQKFSVREKIDIAGIQVQETVKNNSPHMFQIIGKKRSLELQARTEEEKKEWIQVIQATIEKHKQNSETFRAFNSSFSRDDEHCPDSPVPGTGPPDYSSGMDGCSSPGGADSFRKSCKIKREKEKQNCKGCGESFNSITKRRHHCKQCGAVICAKCSEFKLLAENSRHNRVCKECFPLVSSVPGSPGGPTESGDTKKKVEKQNSLSPENCVFSSSLLFLEKGRTWTKVWVSVPRNEPLVMYLQGGSQDGRPPRAIPLPGYEVGLPSAGDKVDLKHVFKLSQSQQVLFFGAEDEDLLQKWKGILSKASRGDPPIVAEDPEKL

Target ORF information:

RefSeq Version	XM_012961791.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis FYVE, RhoGEF and PH domain containing 3 (fgd3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961791.2

ORF Insert Sequence:

ATGGAGCAAG GGACGAGCCC CCTGTGTTGC CCCCATGTTT CTGCGAGGGA CTCGGGGTCG 
GAGAGTGGGG TCCCTTTGGG GGAGCCGGGC AGGACGGCGG AGGAGGAACC CACTGCAGAG 
CTGCCCATGG ATAATCTCAA AATGAGAGAC CTTACAATAA ACGATATTAA CAACCGCGGG 
GGTAACAACG CCTCTGTGAA CAGGACTGAG CGCCCCCCGC AAGAGAACGG AAACCTGACC 
GTCGATCACA ACGACAATGG GAAAATTCCC AACAGGGACA GCGGCATCGA CAGCCCTTCC 
TGCAGCGTGG TCGGGGAGGG GTTCTCCAAC GACGAGAGCG CCGAGCCCAA AAAGCTCTCC 
CTGCCGGTCC ACATGGTGGA AATTGGGATT GTCCGGAAGA ACTCGGACTC GTCGTCTGTG 
GATCAGAAAC GGGACTCGAC CCAAGATGAG GACAGCGACC TGGACGAGGG CAGCAGCGAA 
GAACATGAAG CCATGGAGGT GCCCAAGGCA GAGAGCGTCG ATCCCACCAA ATGTTCTGAG 
CAGCAGAGAC TGTACAACAT CGCCAGAGAG CTGCTACAGA CGGAGGAGGC GTATGTGAAG 
AGGCTGGATC TGCTGGATCA GGTGTTCTGC ACAAGGTTGT TATCTGCCGG GGTCCCCCAG 
GAGGTGATCA ATGGAATATT CTCCAATATA TCCTCCATCT ATCGCTTTCA CTATCAGTTC 
CTCCTTCCCG AACTAAAGGC GAGAATAATG CAAGAGTGGG ATACGAACCC GCGGATCGGG 
GATATCCTAC AGAAACTCGC CCCGTTCCTG AAGATGTACG GGGAATACGT CAAGAACTTC 
GATAAAGCGA TGGATATGGT CAACAGCTGG ACCCAGCGCT CTTCTACCTT TAAGGAGGTA 
GTTCATACAA TACAGAAGGA CCCGGTGTGC GGGAACCTGA CCCTGCAGCA CCACATGTTG 
GAGCCGGTGC AGAGGATTCC CCGCTACGAG CTGCTCCTGA AGGATTATCT GAAGAAACTC 
CCAGACGACT CTCCGGATCG CAAGGATTCA GAAAAGTCCC TGGAGTTAAT TTCTACTGCG 
GCCAATCACT CAAACGCAGC CATCCGCAAA ATGGAGAAGA TGCACAAACT GCTGGAGGTG 
TATGAGCGAC TCGGGGGGGA GGAGGATATA GTTAATCCGG CCAATGAGCT CATTAAGGAG 
GGGCACATAC AGAAACTGTC GGCGAAGAAC GGGACCACGC AGGACCGGTA CCTGTACCTG 
TTTAACAGCA TGCTCCTGTA CTGCGTCCCC AAGCTGCGCC TCATGGGCCA GAAGTTCAGC 
GTCCGGGAGA AGATCGACAT CGCCGGGATC CAGGTACAGG AGACCGTGAA GAACAATTCC 
CCTCATATGT TCCAGATAAT CGGCAAAAAG AGATCCCTGG AGCTGCAGGC GAGGACAGAG 
GAGGAGAAGA AGGAATGGAT ACAGGTCATC CAAGCCACGA TCGAGAAGCA CAAACAGAAC 
AGCGAGACCT TCCGGGCGTT TAACAGTTCC TTTTCCAGAG ACGATGAGCA TTGCCCCGAT 
TCCCCGGTGC CGGGCACGGG CCCCCCGGAT TATTCCTCAG GGATGGACGG CTGCAGCTCT 
CCTGGAGGGG CCGATTCCTT CCGGAAATCC TGTAAGATAA AGCGGGAGAA GGAGAAGCAA 
AACTGCAAGG GCTGCGGGGA GAGTTTCAAC TCCATAACGA AGCGCAGGCA CCACTGCAAG 
CAATGCGGCG CCGTGATTTG CGCAAAGTGC TCGGAGTTCA AGCTGCTGGC GGAGAACAGT 
CGGCATAACC GGGTCTGTAA GGAGTGTTTC CCTCTCGTCT CCTCTGTCCC GGGGAGTCCG 
GGGGGCCCCA CAGAGTCGGG GGACACAAAG AAGAAAGTGG AGAAGCAGAA TTCCTTGTCT 
CCGGAAAACT GTGTGTTCAG CAGTAGCCTC CTGTTCCTGG AGAAGGGGAG AACGTGGACG 
AAAGTGTGGG TGTCGGTGCC CAGGAACGAG CCCCTCGTCA TGTATCTGCA GGGGGGCAGC 
CAGGATGGGC GGCCCCCCCG GGCAATACCG TTGCCAGGAT ACGAAGTGGG TTTGCCAAGC 
GCCGGTGACA AAGTGGACTT GAAACACGTA TTTAAACTGT CGCAGTCGCA GCAAGTTCTG 
TTCTTCGGGG CGGAGGATGA GGATTTGCTG CAGAAGTGGA AGGGGATTCT CAGTAAAGCC 
TCTCGGGGGG ACCCCCCCAT TGTGGCAGAG GATCCAGAAA AGCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fgd3 ( XM_002941737.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002941783.2 Xenopus tropicalis FYVE, RhoGEF and PH domain containing 3 (fgd3), transcript variant X2, mRNA.

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CloneID	OXa09806
Clone ID Related Accession (Same CDS sequence)	XM_012961791.2 , XM_002941737.4
Accession Version	XM_002941737.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	FYVE, RhoGEF and PH domain-containing protein 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683495.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002941737.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAG GGACGAGCCC CCTGTGTTGC CCCCATGTTT CTGCGAGGGA CTCGGGGTCG 
GAGAGTGGGG TCCCTTTGGG GGAGCCGGGC AGGACGGCGG AGGAGGAACC CACTGCAGAG 
CTGCCCATGG ATAATCTCAA AATGAGAGAC CTTACAATAA ACGATATTAA CAACCGCGGG 
GGTAACAACG CCTCTGTGAA CAGGACTGAG CGCCCCCCGC AAGAGAACGG AAACCTGACC 
GTCGATCACA ACGACAATGG GAAAATTCCC AACAGGGACA GCGGCATCGA CAGCCCTTCC 
TGCAGCGTGG TCGGGGAGGG GTTCTCCAAC GACGAGAGCG CCGAGCCCAA AAAGCTCTCC 
CTGCCGGTCC ACATGGTGGA AATTGGGATT GTCCGGAAGA ACTCGGACTC GTCGTCTGTG 
GATCAGAAAC GGGACTCGAC CCAAGATGAG GACAGCGACC TGGACGAGGG CAGCAGCGAA 
GAACATGAAG CCATGGAGGT GCCCAAGGCA GAGAGCGTCG ATCCCACCAA ATGTTCTGAG 
CAGCAGAGAC TGTACAACAT CGCCAGAGAG CTGCTACAGA CGGAGGAGGC GTATGTGAAG 
AGGCTGGATC TGCTGGATCA GGTGTTCTGC ACAAGGTTGT TATCTGCCGG GGTCCCCCAG 
GAGGTGATCA ATGGAATATT CTCCAATATA TCCTCCATCT ATCGCTTTCA CTATCAGTTC 
CTCCTTCCCG AACTAAAGGC GAGAATAATG CAAGAGTGGG ATACGAACCC GCGGATCGGG 
GATATCCTAC AGAAACTCGC CCCGTTCCTG AAGATGTACG GGGAATACGT CAAGAACTTC 
GATAAAGCGA TGGATATGGT CAACAGCTGG ACCCAGCGCT CTTCTACCTT TAAGGAGGTA 
GTTCATACAA TACAGAAGGA CCCGGTGTGC GGGAACCTGA CCCTGCAGCA CCACATGTTG 
GAGCCGGTGC AGAGGATTCC CCGCTACGAG CTGCTCCTGA AGGATTATCT GAAGAAACTC 
CCAGACGACT CTCCGGATCG CAAGGATTCA GAAAAGTCCC TGGAGTTAAT TTCTACTGCG 
GCCAATCACT CAAACGCAGC CATCCGCAAA ATGGAGAAGA TGCACAAACT GCTGGAGGTG 
TATGAGCGAC TCGGGGGGGA GGAGGATATA GTTAATCCGG CCAATGAGCT CATTAAGGAG 
GGGCACATAC AGAAACTGTC GGCGAAGAAC GGGACCACGC AGGACCGGTA CCTGTACCTG 
TTTAACAGCA TGCTCCTGTA CTGCGTCCCC AAGCTGCGCC TCATGGGCCA GAAGTTCAGC 
GTCCGGGAGA AGATCGACAT CGCCGGGATC CAGGTACAGG AGACCGTGAA GAACAATTCC 
CCTCATATGT TCCAGATAAT CGGCAAAAAG AGATCCCTGG AGCTGCAGGC GAGGACAGAG 
GAGGAGAAGA AGGAATGGAT ACAGGTCATC CAAGCCACGA TCGAGAAGCA CAAACAGAAC 
AGCGAGACCT TCCGGGCGTT TAACAGTTCC TTTTCCAGAG ACGATGAGCA TTGCCCCGAT 
TCCCCGGTGC CGGGCACGGG CCCCCCGGAT TATTCCTCAG GGATGGACGG CTGCAGCTCT 
CCTGGAGGGG CCGATTCCTT CCGGAAATCC TGTAAGATAA AGCGGGAGAA GGAGAAGCAA 
AACTGCAAGG GCTGCGGGGA GAGTTTCAAC TCCATAACGA AGCGCAGGCA CCACTGCAAG 
CAATGCGGCG CCGTGATTTG CGCAAAGTGC TCGGAGTTCA AGCTGCTGGC GGAGAACAGT 
CGGCATAACC GGGTCTGTAA GGAGTGTTTC CCTCTCGTCT CCTCTGTCCC GGGGAGTCCG 
GGGGGCCCCA CAGAGTCGGG GGACACAAAG AAGAAAGTGG AGAAGCAGAA TTCCTTGTCT 
CCGGAAAACT GTGTGTTCAG CAGTAGCCTC CTGTTCCTGG AGAAGGGGAG AACGTGGACG 
AAAGTGTGGG TGTCGGTGCC CAGGAACGAG CCCCTCGTCA TGTATCTGCA GGGGGGCAGC 
CAGGATGGGC GGCCCCCCCG GGCAATACCG TTGCCAGGAT ACGAAGTGGG TTTGCCAAGC 
GCCGGTGACA AAGTGGACTT GAAACACGTA TTTAAACTGT CGCAGTCGCA GCAAGTTCTG 
TTCTTCGGGG CGGAGGATGA GGATTTGCTG CAGAAGTGGA AGGGGATTCT CAGTAAAGCC 
TCTCGGGGGG ACCCCCCCAT TGTGGCAGAG GATCCAGAAA AGCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002941783.2
CDS	136..2403
Translation	MEQGTSPLCCPHVSARDSGSESGVPLGEPGRTAEEEPTAELPMDNLKMRDLTINDINNRGGNNASVNRTERPPQENGNLTVDHNDNGKIPNRDSGIDSPSCSVVGEGFSNDESAEPKKLSLPVHMVEIGIVRKNSDSSSVDQKRDSTQDEDSDLDEGSSEEHEAMEVPKAESVDPTKCSEQQRLYNIARELLQTEEAYVKRLDLLDQVFCTRLLSAGVPQEVINGIFSNISSIYRFHYQFLLPELKARIMQEWDTNPRIGDILQKLAPFLKMYGEYVKNFDKAMDMVNSWTQRSSTFKEVVHTIQKDPVCGNLTLQHHMLEPVQRIPRYELLLKDYLKKLPDDSPDRKDSEKSLELISTAANHSNAAIRKMEKMHKLLEVYERLGGEEDIVNPANELIKEGHIQKLSAKNGTTQDRYLYLFNSMLLYCVPKLRLMGQKFSVREKIDIAGIQVQETVKNNSPHMFQIIGKKRSLELQARTEEEKKEWIQVIQATIEKHKQNSETFRAFNSSFSRDDEHCPDSPVPGTGPPDYSSGMDGCSSPGGADSFRKSCKIKREKEKQNCKGCGESFNSITKRRHHCKQCGAVICAKCSEFKLLAENSRHNRVCKECFPLVSSVPGSPGGPTESGDTKKKVEKQNSLSPENCVFSSSLLFLEKGRTWTKVWVSVPRNEPLVMYLQGGSQDGRPPRAIPLPGYEVGLPSAGDKVDLKHVFKLSQSQQVLFFGAEDEDLLQKWKGILSKASRGDPPIVAEDPEKL

Target ORF information:

RefSeq Version	XM_002941737.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis FYVE, RhoGEF and PH domain containing 3 (fgd3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002941737.4

ORF Insert Sequence:

ATGGAGCAAG GGACGAGCCC CCTGTGTTGC CCCCATGTTT CTGCGAGGGA CTCGGGGTCG 
GAGAGTGGGG TCCCTTTGGG GGAGCCGGGC AGGACGGCGG AGGAGGAACC CACTGCAGAG 
CTGCCCATGG ATAATCTCAA AATGAGAGAC CTTACAATAA ACGATATTAA CAACCGCGGG 
GGTAACAACG CCTCTGTGAA CAGGACTGAG CGCCCCCCGC AAGAGAACGG AAACCTGACC 
GTCGATCACA ACGACAATGG GAAAATTCCC AACAGGGACA GCGGCATCGA CAGCCCTTCC 
TGCAGCGTGG TCGGGGAGGG GTTCTCCAAC GACGAGAGCG CCGAGCCCAA AAAGCTCTCC 
CTGCCGGTCC ACATGGTGGA AATTGGGATT GTCCGGAAGA ACTCGGACTC GTCGTCTGTG 
GATCAGAAAC GGGACTCGAC CCAAGATGAG GACAGCGACC TGGACGAGGG CAGCAGCGAA 
GAACATGAAG CCATGGAGGT GCCCAAGGCA GAGAGCGTCG ATCCCACCAA ATGTTCTGAG 
CAGCAGAGAC TGTACAACAT CGCCAGAGAG CTGCTACAGA CGGAGGAGGC GTATGTGAAG 
AGGCTGGATC TGCTGGATCA GGTGTTCTGC ACAAGGTTGT TATCTGCCGG GGTCCCCCAG 
GAGGTGATCA ATGGAATATT CTCCAATATA TCCTCCATCT ATCGCTTTCA CTATCAGTTC 
CTCCTTCCCG AACTAAAGGC GAGAATAATG CAAGAGTGGG ATACGAACCC GCGGATCGGG 
GATATCCTAC AGAAACTCGC CCCGTTCCTG AAGATGTACG GGGAATACGT CAAGAACTTC 
GATAAAGCGA TGGATATGGT CAACAGCTGG ACCCAGCGCT CTTCTACCTT TAAGGAGGTA 
GTTCATACAA TACAGAAGGA CCCGGTGTGC GGGAACCTGA CCCTGCAGCA CCACATGTTG 
GAGCCGGTGC AGAGGATTCC CCGCTACGAG CTGCTCCTGA AGGATTATCT GAAGAAACTC 
CCAGACGACT CTCCGGATCG CAAGGATTCA GAAAAGTCCC TGGAGTTAAT TTCTACTGCG 
GCCAATCACT CAAACGCAGC CATCCGCAAA ATGGAGAAGA TGCACAAACT GCTGGAGGTG 
TATGAGCGAC TCGGGGGGGA GGAGGATATA GTTAATCCGG CCAATGAGCT CATTAAGGAG 
GGGCACATAC AGAAACTGTC GGCGAAGAAC GGGACCACGC AGGACCGGTA CCTGTACCTG 
TTTAACAGCA TGCTCCTGTA CTGCGTCCCC AAGCTGCGCC TCATGGGCCA GAAGTTCAGC 
GTCCGGGAGA AGATCGACAT CGCCGGGATC CAGGTACAGG AGACCGTGAA GAACAATTCC 
CCTCATATGT TCCAGATAAT CGGCAAAAAG AGATCCCTGG AGCTGCAGGC GAGGACAGAG 
GAGGAGAAGA AGGAATGGAT ACAGGTCATC CAAGCCACGA TCGAGAAGCA CAAACAGAAC 
AGCGAGACCT TCCGGGCGTT TAACAGTTCC TTTTCCAGAG ACGATGAGCA TTGCCCCGAT 
TCCCCGGTGC CGGGCACGGG CCCCCCGGAT TATTCCTCAG GGATGGACGG CTGCAGCTCT 
CCTGGAGGGG CCGATTCCTT CCGGAAATCC TGTAAGATAA AGCGGGAGAA GGAGAAGCAA 
AACTGCAAGG GCTGCGGGGA GAGTTTCAAC TCCATAACGA AGCGCAGGCA CCACTGCAAG 
CAATGCGGCG CCGTGATTTG CGCAAAGTGC TCGGAGTTCA AGCTGCTGGC GGAGAACAGT 
CGGCATAACC GGGTCTGTAA GGAGTGTTTC CCTCTCGTCT CCTCTGTCCC GGGGAGTCCG 
GGGGGCCCCA CAGAGTCGGG GGACACAAAG AAGAAAGTGG AGAAGCAGAA TTCCTTGTCT 
CCGGAAAACT GTGTGTTCAG CAGTAGCCTC CTGTTCCTGG AGAAGGGGAG AACGTGGACG 
AAAGTGTGGG TGTCGGTGCC CAGGAACGAG CCCCTCGTCA TGTATCTGCA GGGGGGCAGC 
CAGGATGGGC GGCCCCCCCG GGCAATACCG TTGCCAGGAT ACGAAGTGGG TTTGCCAAGC 
GCCGGTGACA AAGTGGACTT GAAACACGTA TTTAAACTGT CGCAGTCGCA GCAAGTTCTG 
TTCTTCGGGG CGGAGGATGA GGATTTGCTG CAGAAGTGGA AGGGGATTCT CAGTAAAGCC 
TCTCGGGGGG ACCCCCCCAT TGTGGCAGAG GATCCAGAAA AGCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fgd3 ( XM_004914180.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914237.2 Xenopus tropicalis FYVE, RhoGEF and PH domain containing 3 (fgd3), transcript variant X1, mRNA.

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CloneID	OXa57664
Clone ID Related Accession (Same CDS sequence)	XM_004914180.4
Accession Version	XM_004914180.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2691bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	FYVE, RhoGEF and PH domain-containing protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004914180.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAGAA TGACTGGCCC CAGGGCAGAG GGTCGCAGCT GCGCTCCCTC CCAGCTCAGA 
TGTCACATTG AATCGAGTGC GTTCGGGCGG CCGCAGCAAG TTGGCCCCGT ACCATGTATC 
CAGCAAAGCG CAAGGAAGTG GAAGTGCAAA CCTCGGCCAA TCAGAACACT TCCCTCCGAA 
AAACCCCAAG TGCCCCCTAA ACCCGCCCAT CTGAAAGCAT CGGGGAAAGA AATACTAAAC 
CACCGAATTC CTCCTGCCCC CTCAAAGCCA TTGCCCCTGG TACCCAAACC CCCCCGCCGG 
CCTGGGGTTA AAGAGCGGGG CAAGTCTGGA ACCTCCGACA TCAACCTCCT CATCAAGAAG 
TTTGAGAGCC TCAGGCAGCC CGTCCAGTTT GGGAGACACA GTCGGTACCA TTTTAGTGCC 
AGGATGGAGC AAGGGACGAG CCCCCTGTGT TGCCCCCATG TTTCTGCGAG GGACTCGGGG 
TCGGAGAGTG GGGTCCCTTT GGGGGAGCCG GGCAGGACGG CGGAGGAGGA ACCCACTGCA 
GAGCTGCCCA TGGATAATCT CAAAATGAGA GACCTTACAA TAAACGATAT TAACAACCGC 
GGGGGTAACA ACGCCTCTGT GAACAGGACT GAGCGCCCCC CGCAAGAGAA CGGAAACCTG 
ACCGTCGATC ACAACGACAA TGGGAAAATT CCCAACAGGG ACAGCGGCAT CGACAGCCCT 
TCCTGCAGCG TGGTCGGGGA GGGGTTCTCC AACGACGAGA GCGCCGAGCC CAAAAAGCTC 
TCCCTGCCGG TCCACATGGT GGAAATTGGG ATTGTCCGGA AGAACTCGGA CTCGTCGTCT 
GTGGATCAGA AACGGGACTC GACCCAAGAT GAGGACAGCG ACCTGGACGA GGGCAGCAGC 
GAAGAACATG AAGCCATGGA GGTGCCCAAG GCAGAGAGCG TCGATCCCAC CAAATGTTCT 
GAGCAGCAGA GACTGTACAA CATCGCCAGA GAGCTGCTAC AGACGGAGGA GGCGTATGTG 
AAGAGGCTGG ATCTGCTGGA TCAGGTGTTC TGCACAAGGT TGTTATCTGC CGGGGTCCCC 
CAGGAGGTGA TCAATGGAAT ATTCTCCAAT ATATCCTCCA TCTATCGCTT TCACTATCAG 
TTCCTCCTTC CCGAACTAAA GGCGAGAATA ATGCAAGAGT GGGATACGAA CCCGCGGATC 
GGGGATATCC TACAGAAACT CGCCCCGTTC CTGAAGATGT ACGGGGAATA CGTCAAGAAC 
TTCGATAAAG CGATGGATAT GGTCAACAGC TGGACCCAGC GCTCTTCTAC CTTTAAGGAG 
GTAGTTCATA CAATACAGAA GGACCCGGTG TGCGGGAACC TGACCCTGCA GCACCACATG 
TTGGAGCCGG TGCAGAGGAT TCCCCGCTAC GAGCTGCTCC TGAAGGATTA TCTGAAGAAA 
CTCCCAGACG ACTCTCCGGA TCGCAAGGAT TCAGAAAAGT CCCTGGAGTT AATTTCTACT 
GCGGCCAATC ACTCAAACGC AGCCATCCGC AAAATGGAGA AGATGCACAA ACTGCTGGAG 
GTGTATGAGC GACTCGGGGG GGAGGAGGAT ATAGTTAATC CGGCCAATGA GCTCATTAAG 
GAGGGGCACA TACAGAAACT GTCGGCGAAG AACGGGACCA CGCAGGACCG GTACCTGTAC 
CTGTTTAACA GCATGCTCCT GTACTGCGTC CCCAAGCTGC GCCTCATGGG CCAGAAGTTC 
AGCGTCCGGG AGAAGATCGA CATCGCCGGG ATCCAGGTAC AGGAGACCGT GAAGAACAAT 
TCCCCTCATA TGTTCCAGAT AATCGGCAAA AAGAGATCCC TGGAGCTGCA GGCGAGGACA 
GAGGAGGAGA AGAAGGAATG GATACAGGTC ATCCAAGCCA CGATCGAGAA GCACAAACAG 
AACAGCGAGA CCTTCCGGGC GTTTAACAGT TCCTTTTCCA GAGACGATGA GCATTGCCCC 
GATTCCCCGG TGCCGGGCAC GGGCCCCCCG GATTATTCCT CAGGGATGGA CGGCTGCAGC 
TCTCCTGGAG GGGCCGATTC CTTCCGGAAA TCCTGTAAGA TAAAGCGGGA GAAGGAGAAG 
CAAAACTGCA AGGGCTGCGG GGAGAGTTTC AACTCCATAA CGAAGCGCAG GCACCACTGC 
AAGCAATGCG GCGCCGTGAT TTGCGCAAAG TGCTCGGAGT TCAAGCTGCT GGCGGAGAAC 
AGTCGGCATA ACCGGGTCTG TAAGGAGTGT TTCCCTCTCG TCTCCTCTGT CTCGGGGAGT 
CCGGGGGGCA CCACAGAGTC GGGGGACACA AAGAAGAAAG TGGAGAAGCA GAATTCCTTG 
TCTCCGGAAA ACTGTGTGTT CAGCAGTAGC CTCCTGTTCC TGGAGAAGGG GAGAACGTGG 
ACGAAAGTGT GGGTGTCGGT GCCCAGGAAC GAGCCCCTCG TCATGTATCT GCAGGGGGGC 
AGCCAGGATG GGCGGCCCCC CCGGGCAATA CCGTTGCCAG GATACGAAGT GGGTTTGCCA 
AGCGCCGGTG ACAAAGTGGA CTTGAAACAC GTATTTAAAC TGTCGCAGTC GCAGCAAGTT 
CTGTTCTTCG GGGCGGAGGA TGAGGATTTG CTGCAGAAGT GGAAGGGGAT TCTCAGTAAA 
GCCTCTCGGG GGGACCCCCC CATTGTGGCA GAGGATCCAG AAAAGCTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914237.2
CDS	183..2873
Translation	MNRMTGPRAEGRSCAPSQLRCHIESSAFGRPQQVGPVPCIQQSARKWKCKPRPIRTLPSEKPQVPPKPAHLKASGKEILNHRIPPAPSKPLPLVPKPPRRPGVKERGKSGTSDINLLIKKFESLRQPVQFGRHSRYHFSARMEQGTSPLCCPHVSARDSGSESGVPLGEPGRTAEEEPTAELPMDNLKMRDLTINDINNRGGNNASVNRTERPPQENGNLTVDHNDNGKIPNRDSGIDSPSCSVVGEGFSNDESAEPKKLSLPVHMVEIGIVRKNSDSSSVDQKRDSTQDEDSDLDEGSSEEHEAMEVPKAESVDPTKCSEQQRLYNIARELLQTEEAYVKRLDLLDQVFCTRLLSAGVPQEVINGIFSNISSIYRFHYQFLLPELKARIMQEWDTNPRIGDILQKLAPFLKMYGEYVKNFDKAMDMVNSWTQRSSTFKEVVHTIQKDPVCGNLTLQHHMLEPVQRIPRYELLLKDYLKKLPDDSPDRKDSEKSLELISTAANHSNAAIRKMEKMHKLLEVYERLGGEEDIVNPANELIKEGHIQKLSAKNGTTQDRYLYLFNSMLLYCVPKLRLMGQKFSVREKIDIAGIQVQETVKNNSPHMFQIIGKKRSLELQARTEEEKKEWIQVIQATIEKHKQNSETFRAFNSSFSRDDEHCPDSPVPGTGPPDYSSGMDGCSSPGGADSFRKSCKIKREKEKQNCKGCGESFNSITKRRHHCKQCGAVICAKCSEFKLLAENSRHNRVCKECFPLVSSVSGSPGGTTESGDTKKKVEKQNSLSPENCVFSSSLLFLEKGRTWTKVWVSVPRNEPLVMYLQGGSQDGRPPRAIPLPGYEVGLPSAGDKVDLKHVFKLSQSQQVLFFGAEDEDLLQKWKGILSKASRGDPPIVAEDPEKL

Target ORF information:

RefSeq Version	XM_004914180.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis FYVE, RhoGEF and PH domain containing 3 (fgd3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914180.4

ORF Insert Sequence:

ATGAACAGAA TGACTGGCCC CAGGGCAGAG GGTCGCAGCT GCGCTCCCTC CCAGCTCAGA 
TGTCACATTG AATCGAGTGC GTTCGGGCGG CCGCAGCAAG TTGGCCCCGT ACCATGTATC 
CAGCAAAGCG CAAGGAAGTG GAAGTGCAAA CCTCGGCCAA TCAGAACACT TCCCTCCGAA 
AAACCCCAAG TGCCCCCTAA ACCCGCCCAT CTGAAAGCAT CGGGGAAAGA AATACTAAAC 
CACCGAATTC CTCCTGCCCC CTCAAAGCCA TTGCCCCTGG TACCCAAACC CCCCCGCCGG 
CCTGGGGTTA AAGAGCGGGG CAAGTCTGGA ACCTCCGACA TCAACCTCCT CATCAAGAAG 
TTTGAGAGCC TCAGGCAGCC CGTCCAGTTT GGGAGACACA GTCGGTACCA TTTTAGTGCC 
AGGATGGAGC AAGGGACGAG CCCCCTGTGT TGCCCCCATG TTTCTGCGAG GGACTCGGGG 
TCGGAGAGTG GGGTCCCTTT GGGGGAGCCG GGCAGGACGG CGGAGGAGGA ACCCACTGCA 
GAGCTGCCCA TGGATAATCT CAAAATGAGA GACCTTACAA TAAACGATAT TAACAACCGC 
GGGGGTAACA ACGCCTCTGT GAACAGGACT GAGCGCCCCC CGCAAGAGAA CGGAAACCTG 
ACCGTCGATC ACAACGACAA TGGGAAAATT CCCAACAGGG ACAGCGGCAT CGACAGCCCT 
TCCTGCAGCG TGGTCGGGGA GGGGTTCTCC AACGACGAGA GCGCCGAGCC CAAAAAGCTC 
TCCCTGCCGG TCCACATGGT GGAAATTGGG ATTGTCCGGA AGAACTCGGA CTCGTCGTCT 
GTGGATCAGA AACGGGACTC GACCCAAGAT GAGGACAGCG ACCTGGACGA GGGCAGCAGC 
GAAGAACATG AAGCCATGGA GGTGCCCAAG GCAGAGAGCG TCGATCCCAC CAAATGTTCT 
GAGCAGCAGA GACTGTACAA CATCGCCAGA GAGCTGCTAC AGACGGAGGA GGCGTATGTG 
AAGAGGCTGG ATCTGCTGGA TCAGGTGTTC TGCACAAGGT TGTTATCTGC CGGGGTCCCC 
CAGGAGGTGA TCAATGGAAT ATTCTCCAAT ATATCCTCCA TCTATCGCTT TCACTATCAG 
TTCCTCCTTC CCGAACTAAA GGCGAGAATA ATGCAAGAGT GGGATACGAA CCCGCGGATC 
GGGGATATCC TACAGAAACT CGCCCCGTTC CTGAAGATGT ACGGGGAATA CGTCAAGAAC 
TTCGATAAAG CGATGGATAT GGTCAACAGC TGGACCCAGC GCTCTTCTAC CTTTAAGGAG 
GTAGTTCATA CAATACAGAA GGACCCGGTG TGCGGGAACC TGACCCTGCA GCACCACATG 
TTGGAGCCGG TGCAGAGGAT TCCCCGCTAC GAGCTGCTCC TGAAGGATTA TCTGAAGAAA 
CTCCCAGACG ACTCTCCGGA TCGCAAGGAT TCAGAAAAGT CCCTGGAGTT AATTTCTACT 
GCGGCCAATC ACTCAAACGC AGCCATCCGC AAAATGGAGA AGATGCACAA ACTGCTGGAG 
GTGTATGAGC GACTCGGGGG GGAGGAGGAT ATAGTTAATC CGGCCAATGA GCTCATTAAG 
GAGGGGCACA TACAGAAACT GTCGGCGAAG AACGGGACCA CGCAGGACCG GTACCTGTAC 
CTGTTTAACA GCATGCTCCT GTACTGCGTC CCCAAGCTGC GCCTCATGGG CCAGAAGTTC 
AGCGTCCGGG AGAAGATCGA CATCGCCGGG ATCCAGGTAC AGGAGACCGT GAAGAACAAT 
TCCCCTCATA TGTTCCAGAT AATCGGCAAA AAGAGATCCC TGGAGCTGCA GGCGAGGACA 
GAGGAGGAGA AGAAGGAATG GATACAGGTC ATCCAAGCCA CGATCGAGAA GCACAAACAG 
AACAGCGAGA CCTTCCGGGC GTTTAACAGT TCCTTTTCCA GAGACGATGA GCATTGCCCC 
GATTCCCCGG TGCCGGGCAC GGGCCCCCCG GATTATTCCT CAGGGATGGA CGGCTGCAGC 
TCTCCTGGAG GGGCCGATTC CTTCCGGAAA TCCTGTAAGA TAAAGCGGGA GAAGGAGAAG 
CAAAACTGCA AGGGCTGCGG GGAGAGTTTC AACTCCATAA CGAAGCGCAG GCACCACTGC 
AAGCAATGCG GCGCCGTGAT TTGCGCAAAG TGCTCGGAGT TCAAGCTGCT GGCGGAGAAC 
AGTCGGCATA ACCGGGTCTG TAAGGAGTGT TTCCCTCTCG TCTCCTCTGT CTCGGGGAGT 
CCGGGGGGCA CCACAGAGTC GGGGGACACA AAGAAGAAAG TGGAGAAGCA GAATTCCTTG 
TCTCCGGAAA ACTGTGTGTT CAGCAGTAGC CTCCTGTTCC TGGAGAAGGG GAGAACGTGG 
ACGAAAGTGT GGGTGTCGGT GCCCAGGAAC GAGCCCCTCG TCATGTATCT GCAGGGGGGC 
AGCCAGGATG GGCGGCCCCC CCGGGCAATA CCGTTGCCAG GATACGAAGT GGGTTTGCCA 
AGCGCCGGTG ACAAAGTGGA CTTGAAACAC GTATTTAAAC TGTCGCAGTC GCAGCAAGTT 
CTGTTCTTCG GGGCGGAGGA TGAGGATTTG CTGCAGAAGT GGAAGGGGAT TCTCAGTAAA 
GCCTCTCGGG GGGACCCCCC CATTGTGGCA GAGGATCCAG AAAAGCTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fgd3 ( XM_002941737.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002941783.3 Xenopus tropicalis FYVE, RhoGEF and PH domain containing 3 (fgd3), transcript variant X2, mRNA.

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CloneID	OXa57665
Clone ID Related Accession (Same CDS sequence)	XM_002941737.5 , XM_012961791.3
Accession Version	XM_002941737.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	FYVE, RhoGEF and PH domain-containing protein 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002941737.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAG GGACGAGCCC CCTGTGTTGC CCCCATGTTT CTGCGAGGGA CTCGGGGTCG 
GAGAGTGGGG TCCCTTTGGG GGAGCCGGGC AGGACGGCGG AGGAGGAACC CACTGCAGAG 
CTGCCCATGG ATAATCTCAA AATGAGAGAC CTTACAATAA ACGATATTAA CAACCGCGGG 
GGTAACAACG CCTCTGTGAA CAGGACTGAG CGCCCCCCGC AAGAGAACGG AAACCTGACC 
GTCGATCACA ACGACAATGG GAAAATTCCC AACAGGGACA GCGGCATCGA CAGCCCTTCC 
TGCAGCGTGG TCGGGGAGGG GTTCTCCAAC GACGAGAGCG CCGAGCCCAA AAAGCTCTCC 
CTGCCGGTCC ACATGGTGGA AATTGGGATT GTCCGGAAGA ACTCGGACTC GTCGTCTGTG 
GATCAGAAAC GGGACTCGAC CCAAGATGAG GACAGCGACC TGGACGAGGG CAGCAGCGAA 
GAACATGAAG CCATGGAGGT GCCCAAGGCA GAGAGCGTCG ATCCCACCAA ATGTTCTGAG 
CAGCAGAGAC TGTACAACAT CGCCAGAGAG CTGCTACAGA CGGAGGAGGC GTATGTGAAG 
AGGCTGGATC TGCTGGATCA GGTGTTCTGC ACAAGGTTGT TATCTGCCGG GGTCCCCCAG 
GAGGTGATCA ATGGAATATT CTCCAATATA TCCTCCATCT ATCGCTTTCA CTATCAGTTC 
CTCCTTCCCG AACTAAAGGC GAGAATAATG CAAGAGTGGG ATACGAACCC GCGGATCGGG 
GATATCCTAC AGAAACTCGC CCCGTTCCTG AAGATGTACG GGGAATACGT CAAGAACTTC 
GATAAAGCGA TGGATATGGT CAACAGCTGG ACCCAGCGCT CTTCTACCTT TAAGGAGGTA 
GTTCATACAA TACAGAAGGA CCCGGTGTGC GGGAACCTGA CCCTGCAGCA CCACATGTTG 
GAGCCGGTGC AGAGGATTCC CCGCTACGAG CTGCTCCTGA AGGATTATCT GAAGAAACTC 
CCAGACGACT CTCCGGATCG CAAGGATTCA GAAAAGTCCC TGGAGTTAAT TTCTACTGCG 
GCCAATCACT CAAACGCAGC CATCCGCAAA ATGGAGAAGA TGCACAAACT GCTGGAGGTG 
TATGAGCGAC TCGGGGGGGA GGAGGATATA GTTAATCCGG CCAATGAGCT CATTAAGGAG 
GGGCACATAC AGAAACTGTC GGCGAAGAAC GGGACCACGC AGGACCGGTA CCTGTACCTG 
TTTAACAGCA TGCTCCTGTA CTGCGTCCCC AAGCTGCGCC TCATGGGCCA GAAGTTCAGC 
GTCCGGGAGA AGATCGACAT CGCCGGGATC CAGGTACAGG AGACCGTGAA GAACAATTCC 
CCTCATATGT TCCAGATAAT CGGCAAAAAG AGATCCCTGG AGCTGCAGGC GAGGACAGAG 
GAGGAGAAGA AGGAATGGAT ACAGGTCATC CAAGCCACGA TCGAGAAGCA CAAACAGAAC 
AGCGAGACCT TCCGGGCGTT TAACAGTTCC TTTTCCAGAG ACGATGAGCA TTGCCCCGAT 
TCCCCGGTGC CGGGCACGGG CCCCCCGGAT TATTCCTCAG GGATGGACGG CTGCAGCTCT 
CCTGGAGGGG CCGATTCCTT CCGGAAATCC TGTAAGATAA AGCGGGAGAA GGAGAAGCAA 
AACTGCAAGG GCTGCGGGGA GAGTTTCAAC TCCATAACGA AGCGCAGGCA CCACTGCAAG 
CAATGCGGCG CCGTGATTTG CGCAAAGTGC TCGGAGTTCA AGCTGCTGGC GGAGAACAGT 
CGGCATAACC GGGTCTGTAA GGAGTGTTTC CCTCTCGTCT CCTCTGTCTC GGGGAGTCCG 
GGGGGCACCA CAGAGTCGGG GGACACAAAG AAGAAAGTGG AGAAGCAGAA TTCCTTGTCT 
CCGGAAAACT GTGTGTTCAG CAGTAGCCTC CTGTTCCTGG AGAAGGGGAG AACGTGGACG 
AAAGTGTGGG TGTCGGTGCC CAGGAACGAG CCCCTCGTCA TGTATCTGCA GGGGGGCAGC 
CAGGATGGGC GGCCCCCCCG GGCAATACCG TTGCCAGGAT ACGAAGTGGG TTTGCCAAGC 
GCCGGTGACA AAGTGGACTT GAAACACGTA TTTAAACTGT CGCAGTCGCA GCAAGTTCTG 
TTCTTCGGGG CGGAGGATGA GGATTTGCTG CAGAAGTGGA AGGGGATTCT CAGTAAAGCC 
TCTCGGGGGG ACCCCCCCAT TGTGGCAGAG GATCCAGAAA AGCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002941783.3
CDS	136..2403
Translation	MEQGTSPLCCPHVSARDSGSESGVPLGEPGRTAEEEPTAELPMDNLKMRDLTINDINNRGGNNASVNRTERPPQENGNLTVDHNDNGKIPNRDSGIDSPSCSVVGEGFSNDESAEPKKLSLPVHMVEIGIVRKNSDSSSVDQKRDSTQDEDSDLDEGSSEEHEAMEVPKAESVDPTKCSEQQRLYNIARELLQTEEAYVKRLDLLDQVFCTRLLSAGVPQEVINGIFSNISSIYRFHYQFLLPELKARIMQEWDTNPRIGDILQKLAPFLKMYGEYVKNFDKAMDMVNSWTQRSSTFKEVVHTIQKDPVCGNLTLQHHMLEPVQRIPRYELLLKDYLKKLPDDSPDRKDSEKSLELISTAANHSNAAIRKMEKMHKLLEVYERLGGEEDIVNPANELIKEGHIQKLSAKNGTTQDRYLYLFNSMLLYCVPKLRLMGQKFSVREKIDIAGIQVQETVKNNSPHMFQIIGKKRSLELQARTEEEKKEWIQVIQATIEKHKQNSETFRAFNSSFSRDDEHCPDSPVPGTGPPDYSSGMDGCSSPGGADSFRKSCKIKREKEKQNCKGCGESFNSITKRRHHCKQCGAVICAKCSEFKLLAENSRHNRVCKECFPLVSSVSGSPGGTTESGDTKKKVEKQNSLSPENCVFSSSLLFLEKGRTWTKVWVSVPRNEPLVMYLQGGSQDGRPPRAIPLPGYEVGLPSAGDKVDLKHVFKLSQSQQVLFFGAEDEDLLQKWKGILSKASRGDPPIVAEDPEKL

Target ORF information:

RefSeq Version	XM_002941737.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis FYVE, RhoGEF and PH domain containing 3 (fgd3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002941737.5

ORF Insert Sequence:

ATGGAGCAAG GGACGAGCCC CCTGTGTTGC CCCCATGTTT CTGCGAGGGA CTCGGGGTCG 
GAGAGTGGGG TCCCTTTGGG GGAGCCGGGC AGGACGGCGG AGGAGGAACC CACTGCAGAG 
CTGCCCATGG ATAATCTCAA AATGAGAGAC CTTACAATAA ACGATATTAA CAACCGCGGG 
GGTAACAACG CCTCTGTGAA CAGGACTGAG CGCCCCCCGC AAGAGAACGG AAACCTGACC 
GTCGATCACA ACGACAATGG GAAAATTCCC AACAGGGACA GCGGCATCGA CAGCCCTTCC 
TGCAGCGTGG TCGGGGAGGG GTTCTCCAAC GACGAGAGCG CCGAGCCCAA AAAGCTCTCC 
CTGCCGGTCC ACATGGTGGA AATTGGGATT GTCCGGAAGA ACTCGGACTC GTCGTCTGTG 
GATCAGAAAC GGGACTCGAC CCAAGATGAG GACAGCGACC TGGACGAGGG CAGCAGCGAA 
GAACATGAAG CCATGGAGGT GCCCAAGGCA GAGAGCGTCG ATCCCACCAA ATGTTCTGAG 
CAGCAGAGAC TGTACAACAT CGCCAGAGAG CTGCTACAGA CGGAGGAGGC GTATGTGAAG 
AGGCTGGATC TGCTGGATCA GGTGTTCTGC ACAAGGTTGT TATCTGCCGG GGTCCCCCAG 
GAGGTGATCA ATGGAATATT CTCCAATATA TCCTCCATCT ATCGCTTTCA CTATCAGTTC 
CTCCTTCCCG AACTAAAGGC GAGAATAATG CAAGAGTGGG ATACGAACCC GCGGATCGGG 
GATATCCTAC AGAAACTCGC CCCGTTCCTG AAGATGTACG GGGAATACGT CAAGAACTTC 
GATAAAGCGA TGGATATGGT CAACAGCTGG ACCCAGCGCT CTTCTACCTT TAAGGAGGTA 
GTTCATACAA TACAGAAGGA CCCGGTGTGC GGGAACCTGA CCCTGCAGCA CCACATGTTG 
GAGCCGGTGC AGAGGATTCC CCGCTACGAG CTGCTCCTGA AGGATTATCT GAAGAAACTC 
CCAGACGACT CTCCGGATCG CAAGGATTCA GAAAAGTCCC TGGAGTTAAT TTCTACTGCG 
GCCAATCACT CAAACGCAGC CATCCGCAAA ATGGAGAAGA TGCACAAACT GCTGGAGGTG 
TATGAGCGAC TCGGGGGGGA GGAGGATATA GTTAATCCGG CCAATGAGCT CATTAAGGAG 
GGGCACATAC AGAAACTGTC GGCGAAGAAC GGGACCACGC AGGACCGGTA CCTGTACCTG 
TTTAACAGCA TGCTCCTGTA CTGCGTCCCC AAGCTGCGCC TCATGGGCCA GAAGTTCAGC 
GTCCGGGAGA AGATCGACAT CGCCGGGATC CAGGTACAGG AGACCGTGAA GAACAATTCC 
CCTCATATGT TCCAGATAAT CGGCAAAAAG AGATCCCTGG AGCTGCAGGC GAGGACAGAG 
GAGGAGAAGA AGGAATGGAT ACAGGTCATC CAAGCCACGA TCGAGAAGCA CAAACAGAAC 
AGCGAGACCT TCCGGGCGTT TAACAGTTCC TTTTCCAGAG ACGATGAGCA TTGCCCCGAT 
TCCCCGGTGC CGGGCACGGG CCCCCCGGAT TATTCCTCAG GGATGGACGG CTGCAGCTCT 
CCTGGAGGGG CCGATTCCTT CCGGAAATCC TGTAAGATAA AGCGGGAGAA GGAGAAGCAA 
AACTGCAAGG GCTGCGGGGA GAGTTTCAAC TCCATAACGA AGCGCAGGCA CCACTGCAAG 
CAATGCGGCG CCGTGATTTG CGCAAAGTGC TCGGAGTTCA AGCTGCTGGC GGAGAACAGT 
CGGCATAACC GGGTCTGTAA GGAGTGTTTC CCTCTCGTCT CCTCTGTCTC GGGGAGTCCG 
GGGGGCACCA CAGAGTCGGG GGACACAAAG AAGAAAGTGG AGAAGCAGAA TTCCTTGTCT 
CCGGAAAACT GTGTGTTCAG CAGTAGCCTC CTGTTCCTGG AGAAGGGGAG AACGTGGACG 
AAAGTGTGGG TGTCGGTGCC CAGGAACGAG CCCCTCGTCA TGTATCTGCA GGGGGGCAGC 
CAGGATGGGC GGCCCCCCCG GGCAATACCG TTGCCAGGAT ACGAAGTGGG TTTGCCAAGC 
GCCGGTGACA AAGTGGACTT GAAACACGTA TTTAAACTGT CGCAGTCGCA GCAAGTTCTG 
TTCTTCGGGG CGGAGGATGA GGATTTGCTG CAGAAGTGGA AGGGGATTCT CAGTAAAGCC 
TCTCGGGGGG ACCCCCCCAT TGTGGCAGAG GATCCAGAAA AGCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fgd3 ( XM_012961791.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817245.2 Xenopus tropicalis FYVE, RhoGEF and PH domain containing 3 (fgd3), transcript variant X3, mRNA.

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CloneID	OXa57665
Clone ID Related Accession (Same CDS sequence)	XM_002941737.5 , XM_012961791.3
Accession Version	XM_012961791.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	FYVE, RhoGEF and PH domain-containing protein 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961791.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAG GGACGAGCCC CCTGTGTTGC CCCCATGTTT CTGCGAGGGA CTCGGGGTCG 
GAGAGTGGGG TCCCTTTGGG GGAGCCGGGC AGGACGGCGG AGGAGGAACC CACTGCAGAG 
CTGCCCATGG ATAATCTCAA AATGAGAGAC CTTACAATAA ACGATATTAA CAACCGCGGG 
GGTAACAACG CCTCTGTGAA CAGGACTGAG CGCCCCCCGC AAGAGAACGG AAACCTGACC 
GTCGATCACA ACGACAATGG GAAAATTCCC AACAGGGACA GCGGCATCGA CAGCCCTTCC 
TGCAGCGTGG TCGGGGAGGG GTTCTCCAAC GACGAGAGCG CCGAGCCCAA AAAGCTCTCC 
CTGCCGGTCC ACATGGTGGA AATTGGGATT GTCCGGAAGA ACTCGGACTC GTCGTCTGTG 
GATCAGAAAC GGGACTCGAC CCAAGATGAG GACAGCGACC TGGACGAGGG CAGCAGCGAA 
GAACATGAAG CCATGGAGGT GCCCAAGGCA GAGAGCGTCG ATCCCACCAA ATGTTCTGAG 
CAGCAGAGAC TGTACAACAT CGCCAGAGAG CTGCTACAGA CGGAGGAGGC GTATGTGAAG 
AGGCTGGATC TGCTGGATCA GGTGTTCTGC ACAAGGTTGT TATCTGCCGG GGTCCCCCAG 
GAGGTGATCA ATGGAATATT CTCCAATATA TCCTCCATCT ATCGCTTTCA CTATCAGTTC 
CTCCTTCCCG AACTAAAGGC GAGAATAATG CAAGAGTGGG ATACGAACCC GCGGATCGGG 
GATATCCTAC AGAAACTCGC CCCGTTCCTG AAGATGTACG GGGAATACGT CAAGAACTTC 
GATAAAGCGA TGGATATGGT CAACAGCTGG ACCCAGCGCT CTTCTACCTT TAAGGAGGTA 
GTTCATACAA TACAGAAGGA CCCGGTGTGC GGGAACCTGA CCCTGCAGCA CCACATGTTG 
GAGCCGGTGC AGAGGATTCC CCGCTACGAG CTGCTCCTGA AGGATTATCT GAAGAAACTC 
CCAGACGACT CTCCGGATCG CAAGGATTCA GAAAAGTCCC TGGAGTTAAT TTCTACTGCG 
GCCAATCACT CAAACGCAGC CATCCGCAAA ATGGAGAAGA TGCACAAACT GCTGGAGGTG 
TATGAGCGAC TCGGGGGGGA GGAGGATATA GTTAATCCGG CCAATGAGCT CATTAAGGAG 
GGGCACATAC AGAAACTGTC GGCGAAGAAC GGGACCACGC AGGACCGGTA CCTGTACCTG 
TTTAACAGCA TGCTCCTGTA CTGCGTCCCC AAGCTGCGCC TCATGGGCCA GAAGTTCAGC 
GTCCGGGAGA AGATCGACAT CGCCGGGATC CAGGTACAGG AGACCGTGAA GAACAATTCC 
CCTCATATGT TCCAGATAAT CGGCAAAAAG AGATCCCTGG AGCTGCAGGC GAGGACAGAG 
GAGGAGAAGA AGGAATGGAT ACAGGTCATC CAAGCCACGA TCGAGAAGCA CAAACAGAAC 
AGCGAGACCT TCCGGGCGTT TAACAGTTCC TTTTCCAGAG ACGATGAGCA TTGCCCCGAT 
TCCCCGGTGC CGGGCACGGG CCCCCCGGAT TATTCCTCAG GGATGGACGG CTGCAGCTCT 
CCTGGAGGGG CCGATTCCTT CCGGAAATCC TGTAAGATAA AGCGGGAGAA GGAGAAGCAA 
AACTGCAAGG GCTGCGGGGA GAGTTTCAAC TCCATAACGA AGCGCAGGCA CCACTGCAAG 
CAATGCGGCG CCGTGATTTG CGCAAAGTGC TCGGAGTTCA AGCTGCTGGC GGAGAACAGT 
CGGCATAACC GGGTCTGTAA GGAGTGTTTC CCTCTCGTCT CCTCTGTCTC GGGGAGTCCG 
GGGGGCACCA CAGAGTCGGG GGACACAAAG AAGAAAGTGG AGAAGCAGAA TTCCTTGTCT 
CCGGAAAACT GTGTGTTCAG CAGTAGCCTC CTGTTCCTGG AGAAGGGGAG AACGTGGACG 
AAAGTGTGGG TGTCGGTGCC CAGGAACGAG CCCCTCGTCA TGTATCTGCA GGGGGGCAGC 
CAGGATGGGC GGCCCCCCCG GGCAATACCG TTGCCAGGAT ACGAAGTGGG TTTGCCAAGC 
GCCGGTGACA AAGTGGACTT GAAACACGTA TTTAAACTGT CGCAGTCGCA GCAAGTTCTG 
TTCTTCGGGG CGGAGGATGA GGATTTGCTG CAGAAGTGGA AGGGGATTCT CAGTAAAGCC 
TCTCGGGGGG ACCCCCCCAT TGTGGCAGAG GATCCAGAAA AGCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817245.2
CDS	520..2787
Translation	MEQGTSPLCCPHVSARDSGSESGVPLGEPGRTAEEEPTAELPMDNLKMRDLTINDINNRGGNNASVNRTERPPQENGNLTVDHNDNGKIPNRDSGIDSPSCSVVGEGFSNDESAEPKKLSLPVHMVEIGIVRKNSDSSSVDQKRDSTQDEDSDLDEGSSEEHEAMEVPKAESVDPTKCSEQQRLYNIARELLQTEEAYVKRLDLLDQVFCTRLLSAGVPQEVINGIFSNISSIYRFHYQFLLPELKARIMQEWDTNPRIGDILQKLAPFLKMYGEYVKNFDKAMDMVNSWTQRSSTFKEVVHTIQKDPVCGNLTLQHHMLEPVQRIPRYELLLKDYLKKLPDDSPDRKDSEKSLELISTAANHSNAAIRKMEKMHKLLEVYERLGGEEDIVNPANELIKEGHIQKLSAKNGTTQDRYLYLFNSMLLYCVPKLRLMGQKFSVREKIDIAGIQVQETVKNNSPHMFQIIGKKRSLELQARTEEEKKEWIQVIQATIEKHKQNSETFRAFNSSFSRDDEHCPDSPVPGTGPPDYSSGMDGCSSPGGADSFRKSCKIKREKEKQNCKGCGESFNSITKRRHHCKQCGAVICAKCSEFKLLAENSRHNRVCKECFPLVSSVSGSPGGTTESGDTKKKVEKQNSLSPENCVFSSSLLFLEKGRTWTKVWVSVPRNEPLVMYLQGGSQDGRPPRAIPLPGYEVGLPSAGDKVDLKHVFKLSQSQQVLFFGAEDEDLLQKWKGILSKASRGDPPIVAEDPEKL

Target ORF information:

RefSeq Version	XM_012961791.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis FYVE, RhoGEF and PH domain containing 3 (fgd3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961791.3

ORF Insert Sequence:

ATGGAGCAAG GGACGAGCCC CCTGTGTTGC CCCCATGTTT CTGCGAGGGA CTCGGGGTCG 
GAGAGTGGGG TCCCTTTGGG GGAGCCGGGC AGGACGGCGG AGGAGGAACC CACTGCAGAG 
CTGCCCATGG ATAATCTCAA AATGAGAGAC CTTACAATAA ACGATATTAA CAACCGCGGG 
GGTAACAACG CCTCTGTGAA CAGGACTGAG CGCCCCCCGC AAGAGAACGG AAACCTGACC 
GTCGATCACA ACGACAATGG GAAAATTCCC AACAGGGACA GCGGCATCGA CAGCCCTTCC 
TGCAGCGTGG TCGGGGAGGG GTTCTCCAAC GACGAGAGCG CCGAGCCCAA AAAGCTCTCC 
CTGCCGGTCC ACATGGTGGA AATTGGGATT GTCCGGAAGA ACTCGGACTC GTCGTCTGTG 
GATCAGAAAC GGGACTCGAC CCAAGATGAG GACAGCGACC TGGACGAGGG CAGCAGCGAA 
GAACATGAAG CCATGGAGGT GCCCAAGGCA GAGAGCGTCG ATCCCACCAA ATGTTCTGAG 
CAGCAGAGAC TGTACAACAT CGCCAGAGAG CTGCTACAGA CGGAGGAGGC GTATGTGAAG 
AGGCTGGATC TGCTGGATCA GGTGTTCTGC ACAAGGTTGT TATCTGCCGG GGTCCCCCAG 
GAGGTGATCA ATGGAATATT CTCCAATATA TCCTCCATCT ATCGCTTTCA CTATCAGTTC 
CTCCTTCCCG AACTAAAGGC GAGAATAATG CAAGAGTGGG ATACGAACCC GCGGATCGGG 
GATATCCTAC AGAAACTCGC CCCGTTCCTG AAGATGTACG GGGAATACGT CAAGAACTTC 
GATAAAGCGA TGGATATGGT CAACAGCTGG ACCCAGCGCT CTTCTACCTT TAAGGAGGTA 
GTTCATACAA TACAGAAGGA CCCGGTGTGC GGGAACCTGA CCCTGCAGCA CCACATGTTG 
GAGCCGGTGC AGAGGATTCC CCGCTACGAG CTGCTCCTGA AGGATTATCT GAAGAAACTC 
CCAGACGACT CTCCGGATCG CAAGGATTCA GAAAAGTCCC TGGAGTTAAT TTCTACTGCG 
GCCAATCACT CAAACGCAGC CATCCGCAAA ATGGAGAAGA TGCACAAACT GCTGGAGGTG 
TATGAGCGAC TCGGGGGGGA GGAGGATATA GTTAATCCGG CCAATGAGCT CATTAAGGAG 
GGGCACATAC AGAAACTGTC GGCGAAGAAC GGGACCACGC AGGACCGGTA CCTGTACCTG 
TTTAACAGCA TGCTCCTGTA CTGCGTCCCC AAGCTGCGCC TCATGGGCCA GAAGTTCAGC 
GTCCGGGAGA AGATCGACAT CGCCGGGATC CAGGTACAGG AGACCGTGAA GAACAATTCC 
CCTCATATGT TCCAGATAAT CGGCAAAAAG AGATCCCTGG AGCTGCAGGC GAGGACAGAG 
GAGGAGAAGA AGGAATGGAT ACAGGTCATC CAAGCCACGA TCGAGAAGCA CAAACAGAAC 
AGCGAGACCT TCCGGGCGTT TAACAGTTCC TTTTCCAGAG ACGATGAGCA TTGCCCCGAT 
TCCCCGGTGC CGGGCACGGG CCCCCCGGAT TATTCCTCAG GGATGGACGG CTGCAGCTCT 
CCTGGAGGGG CCGATTCCTT CCGGAAATCC TGTAAGATAA AGCGGGAGAA GGAGAAGCAA 
AACTGCAAGG GCTGCGGGGA GAGTTTCAAC TCCATAACGA AGCGCAGGCA CCACTGCAAG 
CAATGCGGCG CCGTGATTTG CGCAAAGTGC TCGGAGTTCA AGCTGCTGGC GGAGAACAGT 
CGGCATAACC GGGTCTGTAA GGAGTGTTTC CCTCTCGTCT CCTCTGTCTC GGGGAGTCCG 
GGGGGCACCA CAGAGTCGGG GGACACAAAG AAGAAAGTGG AGAAGCAGAA TTCCTTGTCT 
CCGGAAAACT GTGTGTTCAG CAGTAGCCTC CTGTTCCTGG AGAAGGGGAG AACGTGGACG 
AAAGTGTGGG TGTCGGTGCC CAGGAACGAG CCCCTCGTCA TGTATCTGCA GGGGGGCAGC 
CAGGATGGGC GGCCCCCCCG GGCAATACCG TTGCCAGGAT ACGAAGTGGG TTTGCCAAGC 
GCCGGTGACA AAGTGGACTT GAAACACGTA TTTAAACTGT CGCAGTCGCA GCAAGTTCTG 
TTCTTCGGGG CGGAGGATGA GGATTTGCTG CAGAAGTGGA AGGGGATTCT CAGTAAAGCC 
TCTCGGGGGG ACCCCCCCAT TGTGGCAGAG GATCCAGAAA AGCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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fgd3 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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