ano8 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ano8
Entrez Gene ID	100491780
Full Name	anoctamin 8
Synonyms	tmem16h
General protein information	Preferred Names anoctamin 8 Names anoctamin-8
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004911016.3	XP_004911073.1	anoctamin-8 isoform X1
XM_004911017.3	XP_004911074.1	anoctamin-8 isoform X1
XM_004911016.4	XP_004911073.1	anoctamin-8 isoform X1
XM_002936335.5	XP_002936381.1	anoctamin-8 isoform X2
XM_002936335.4	XP_002936381.1	anoctamin-8 isoform X2
XM_004911017.4	XP_004911074.1	anoctamin-8 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ano8	XP_006253027.1
Gallus gallus (chicken)	ANO8	XP_427467.4
Mus musculus (house mouse)	Ano8	NP_001158151.1
Xenopus tropicalis (tropical clawed frog)	ano8	XP_004911073.1
Canis lupus familiaris (dog)	ANO8	XP_003639823.1
Bos taurus (cattle)	ANO8	XP_002688590.1
Caenorhabditis elegans (roundworm)	anoh-2	NP_001255820.1
Homo sapiens (human)	ANO8	NP_066010.1
Pan troglodytes (chimpanzee)	ANO8	XP_003953455.1
Danio rerio (zebrafish)	ano8	XP_691248.6

Gene Ontology

The following ano8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ano8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa04340	XM_004911016.4 Latest version!	Xenopus tropicalis anoctamin 8 (ano8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa04340	XM_004911017.4 Latest version!	Xenopus tropicalis anoctamin 8 (ano8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa04341	XM_002936335.5 Latest version!	Xenopus tropicalis anoctamin 8 (ano8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OXa04340	XM_004911016.3	Xenopus tropicalis anoctamin 8 (ano8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa04341	XM_002936335.4	Xenopus tropicalis anoctamin 8 (ano8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa04340	XM_004911017.3	Xenopus tropicalis anoctamin 8 (ano8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ano8 ( XM_004911016.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911073.1 Xenopus tropicalis anoctamin 8 (ano8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa04340
Clone ID Related Accession (Same CDS sequence)	XM_004911016.3 , XM_004911017.3 , XM_004911016.4 , XM_004911017.4
Accession Version	XM_004911016.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	anoctamin-8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004911016.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGACA CAGAGCCGGA CAGAGGCAAG AAAGCCCAGC CGGAAGGGGA GCGAGCAGAA 
CCGGCTGGCA GCCAGCATGG CACGACTGGA GTACTGGATA AACTTTTTGG GAAGCGTCTT 
CTGCAAGCTG GTCGATATAT CACATCCCAC AAATCCTGGA TGAGGACTGT ACCCACAGAA 
AACTGTGACG TAGTTATGAC GTTCTCTGAT AACACGGATG ACCACACTTT GCTGTGGCTA 
CTGAATCATG TACGCCTGGG GTTACCTGAA CTTATTATGC AGATTCGACA CCACAGACAC 
ACTGGGATCT ACGCCTTCTT TGTAACTGCC ACATATGAAA ATTTGCTCCG CGGTGCGGAG 
GAGATTGGTC TTCGAAAACC AGTTAAAGCA GAGTTTGGAG GTGGAACACG AGTCTTCTCA 
TGCGAAGAGG ATTATATTTA TCAGAACATT GAAAATGAAC TCATTTTTTT CACATCTCAG 
GAACGTCAAA GCATCATCCG CTACTGGATG GAAAACCTCC GAGCCAAACA AGGCGAATCC 
CTGCACAATA TCCACTTCCT TGAGGGCCAG CCAATCATCC CAGAGTTGAC AGCCCGTGGG 
GTCATCCAGC AAGTGTTTCC CATCCATGAA CAAAGAATAT TAAGCCGACT TATGAGGTCC 
TGGGTCCAAG CAATCTGCGA AAAACAACCC CTTGATGAAA TCTGTGACTA TTTTGGGGTG 
AAAGTTGCCA TGTATTTTTC CTGGATGGGT TTCTACACCT CCTCCCTTGT CTATCCAGCT 
GTCTTTGGCA TGATGCTGTG GATGATCACT GAGTCTGACC AGACAAGCCG TGACATTTGC 
TGTGTTGTCT TTGCCCTTTT CAATGTTGTT TGGGCTACAT TATTCTTAGA GGGATGGAAA 
AGGAGAGGGG CAGAGTTGGC GTACAAGTGG GGGACACTGG ACACCCCTGC TGAATTTATT 
GAGGAGCCAA GGCCCCAATT CAGGGGTGTC AAGAGGATCA GTCCAGTCAC TAACTGCGAA 
GAGTTTTATT ACCCGCCGTG GAAACGCCTT TTGTTCCAGT CCTTTGTCAG TCTCCCTGTC 
TGTGTCTCCT GTCTGTGTTT TGTCTTTGTG GTCATGCTTG CCTGCTTTGA GCTTCAGGAG 
TTTATACTTA GTATCAAGGA GCTCCCACGG GTTGTACGTT TCTTTCCCAA GATTCTCCTG 
GCCGTTATAG TTAGCTCATG TGACGAAGTT TACAGGAAGA TTGCCTACTG GCTAAATGAT 
ATGGAAAATT ATCGTTTACA GAGTGCCTAC GAGAAACATC TCATCATTAA AATTGTTTTA 
TTCCAGTTTG TAAATTCCTA CCTGAGTCTT TTCTACATTG GTTTCTACCT CAAGGACATG 
GAGCGACTGA AAGAGCTGTT CCTCGTTTTG TCTCTCCCCC ATAGCTTCAC TCGTCAGGTT 
AAAGATGCCC TCCTCCCTTT CCTCTCACTC CAGTTTCACC TGTTCCTCAT CGTCCTCAGG 
GCACTTCTCA GATCAGCCTG GTGCCTCTTC AAATCTAAAA TGCTTGCCAC GCTCCTGGTC 
ATTCGCCAGT TCCTGCAGAA TGTGAAAGAA GTGTCCCAAC CTCATCTATA CCAGAAACTA 
CAACGGGGAG AGCTGACTCC ACGGTCAGTC TGGCTCTTTG TTCGCTATGC CCTGAAGAGG 
TTGTTTCAAA AATATTCTCT CCCTCAGCAC CCATTAAGGA CTCCTGGGAA CCCAATGGAG 
ACGGGTGCAG GTGGGCGCAA GAAGTGCCTC AATGGAGGGT GTGGTGTACC AGAAGAGGAG 
GAGGGAGTGG TCTCTGAAGA TGAGGAAGAG GAGGAGGAAG AAAGTATTAT GGATTGTGGA 
CTCAAATTAA AAAGGGTTAG TTTTTTAGAG AAAGGGGAGA GGAGAGAGGA TGTTGGTCTA 
GTTCCTCAGC AAGAAGATGA GGGTGCCTTT TTACAGGAGG GTAGTCCTAC TCTTATGGAA 
AGAGGTAGCG ACCCAGCCTC AGTTTTTCAG CTGGAAGATG AAGAGGATGA GGAACAAGAA 
GGAGCAAGTG GTGGAGTACT GCAGGAGGGT TTAGAGCAAG AGTCCGGGGT AAAGCTGAGG 
CAGCACAGAA GGAGGAGAAA GAGGGTAATT CTGGAGGAAG AACAAAGTAG AGAAGATGAG 
GAGGATGATA CAGAGATGAA GCAGAAAAGA GAATCATGGA TTGACCCTCC AGAGGACAAT 
TATCCAACTA AGCTTTCTCA AGCTGAGGTG GAGAGTTGTA TGAAGAAATA TGAGGACACT 
TTCAACGATT ACCAAGAGAT GTTTGTGCAG TTTGGGTATG TGGTCCTCTT TTCCTCGGCC 
TTCCCCTTGG CTGCTGTCTG TGCCCTGATC AATAACATAA TCGAGATCCG CAGTGATGCC 
TTTAAGCTGT GCACTGGGCT TCAGAGACCA TTTGGGCGTC GTGTAGATGG CATAGGACAG 
TGGCAAAATG TAATGGAGGC GATGGGGGTT ATAGCCATTA TTGTAAACTG CTACTTAATG 
GGCCAGTGTG GACAGCTTCA AAGACTGCTA CCCTGGCTGG GGCCCGAAAC AACAATCATA 
TTTATTGTGG TGCTTGAGCA TTTTGCTTTG CTCCTGAAGT ATATAATACA CGTTGTGATC 
CCAGACATCC CTGGATGGGT GGCAGAGGAG ATGGCAAAAC TGGAATATCA AAGAAGAGAA 
GCTTTTAAGA AACATGAGCG ACAAGCTCAG CATCGCTACC AGCAGCAACA GCGCAGGAAG 
CGTGAGGAGG AGGAGAGACA GCGCCATGCT GATTACCATG CGAGGAAAGA GCGAGAATCT 
CGTGGAGGGG ACGATGGAAA AAACTCTGGA CAAGATTCCC AGAATCATGA AAAGAACTCA 
AGCAAAACCA AGCAAGGTGG CCCCCCTGGA GGGAGCAGCC ATGATAAGCC GAAAAGACCC 
AGCTCCCTCT TGGCCAGCAA TAATGTGATG AAACTTAAAC AAATTATTCC CCTGCAAAGC 
AAGTTCTTAT CAGGGACCAA TGCCAAATCT CCACAGTCCC CTCCAGCGAC AGACACCAAA 
ATCCCCAGCT TTCTGAGCTT CAAATTTCTA AAATCCCCTG AAACAAAAAG AGAATCCTCC 
CAAGAGAGAG CCCATTCTCC TACAAAGCCT TTCCACCCAG GCAAGCTCTT TAACTTTGGC 
GGAAAGGGTG AGGGACAGGT GGGTGGTAAT GGTATTGGTC CACCCATATC AACACAGCCA 
AGAGGAGCAA CAGCTGGGAC TGCCTCCTCT GATGGTACAG TGCCAGGTAA GTCCCAGCTA 
AATGGGATTT CAGAGGATAG ACCCACGGAG GAGGGTGAAG AACAAACCAA GGCACAAAAA 
CAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911073.1
CDS	515..3880
Translation	MPDTEPDRGKKAQPEGERAEPAGSQHGTTGVLDKLFGKRLLQAGRYITSHKSWMRTVPTENCDVVMTFSDNTDDHTLLWLLNHVRLGLPELIMQIRHHRHTGIYAFFVTATYENLLRGAEEIGLRKPVKAEFGGGTRVFSCEEDYIYQNIENELIFFTSQERQSIIRYWMENLRAKQGESLHNIHFLEGQPIIPELTARGVIQQVFPIHEQRILSRLMRSWVQAICEKQPLDEICDYFGVKVAMYFSWMGFYTSSLVYPAVFGMMLWMITESDQTSRDICCVVFALFNVVWATLFLEGWKRRGAELAYKWGTLDTPAEFIEEPRPQFRGVKRISPVTNCEEFYYPPWKRLLFQSFVSLPVCVSCLCFVFVVMLACFELQEFILSIKELPRVVRFFPKILLAVIVSSCDEVYRKIAYWLNDMENYRLQSAYEKHLIIKIVLFQFVNSYLSLFYIGFYLKDMERLKELFLVLSLPHSFTRQVKDALLPFLSLQFHLFLIVLRALLRSAWCLFKSKMLATLLVIRQFLQNVKEVSQPHLYQKLQRGELTPRSVWLFVRYALKRLFQKYSLPQHPLRTPGNPMETGAGGRKKCLNGGCGVPEEEEGVVSEDEEEEEEESIMDCGLKLKRVSFLEKGERREDVGLVPQQEDEGAFLQEGSPTLMERGSDPASVFQLEDEEDEEQEGASGGVLQEGLEQESGVKLRQHRRRRKRVILEEEQSREDEEDDTEMKQKRESWIDPPEDNYPTKLSQAEVESCMKKYEDTFNDYQEMFVQFGYVVLFSSAFPLAAVCALINNIIEIRSDAFKLCTGLQRPFGRRVDGIGQWQNVMEAMGVIAIIVNCYLMGQCGQLQRLLPWLGPETTIIFIVVLEHFALLLKYIIHVVIPDIPGWVAEEMAKLEYQRREAFKKHERQAQHRYQQQQRRKREEEERQRHADYHARKERESRGGDDGKNSGQDSQNHEKNSSKTKQGGPPGGSSHDKPKRPSSLLASNNVMKLKQIIPLQSKFLSGTNAKSPQSPPATDTKIPSFLSFKFLKSPETKRESSQERAHSPTKPFHPGKLFNFGGKGEGQVGGNGIGPPISTQPRGATAGTASSDGTVPGKSQLNGISEDRPTEEGEEQTKAQKQ

Target ORF information:

RefSeq Version	XM_004911016.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis anoctamin 8 (ano8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911016.4

ORF Insert Sequence:

ATGCCGGACA CAGAGCCGGA CAGAGGCAAG AAAGCCCAGC CGGAAGGGGA GCGAGCAGAA 
CCGGCTGGCA GCCAGCATGG CACGACTGGA GTACTGGATA AACTTTTTGG GAAGCGTCTT 
CTGCAAGCTG GTCGATATAT CACATCCCAC AAATCCTGGA TGAGGACTGT ACCCACAGAA 
AACTGTGACG TAGTTATGAC GTTCTCTGAT AACACGGATG ACCACACTTT GCTGTGGCTA 
CTGAATCATG TACGCCTGGG GTTACCTGAA CTTATTATGC AGATTCGACA CCACAGACAC 
ACTGGGATCT ACGCCTTCTT TGTAACTGCC ACATATGAAA ATTTGCTCCG CGGTGCGGAG 
GAGATTGGTC TTCGAAAACC AGTTAAAGCA GAGTTTGGAG GTGGAACACG AGTCTTCTCA 
TGCGAAGAGG ATTATATTTA TCAGAACATT GAAAATGAAC TCATTTTTTT CACATCTCAG 
GAACGTCAAA GCATCATCCG CTACTGGATG GAAAACCTCC GAGCCAAACA AGGCGAATCC 
CTGCACAATA TCCACTTCCT TGAGGGCCAG CCAATCATCC CAGAGTTGAC AGCCCGTGGG 
GTCATCCAGC AAGTGTTTCC CATCCATGAA CAAAGAATAT TAAGCCGACT TATGAGGTCC 
TGGGTCCAAG CAATCTGCGA AAAACAACCC CTTGATGAAA TCTGTGACTA TTTTGGGGTG 
AAAGTTGCCA TGTATTTTTC CTGGATGGGT TTCTACACCT CCTCCCTTGT CTATCCAGCT 
GTCTTTGGCA TGATGCTGTG GATGATCACT GAGTCTGACC AGACAAGCCG TGACATTTGC 
TGTGTTGTCT TTGCCCTTTT CAATGTTGTT TGGGCTACAT TATTCTTAGA GGGATGGAAA 
AGGAGAGGGG CAGAGTTGGC GTACAAGTGG GGGACACTGG ACACCCCTGC TGAATTTATT 
GAGGAGCCAA GGCCCCAATT CAGGGGTGTC AAGAGGATCA GTCCAGTCAC TAACTGCGAA 
GAGTTTTATT ACCCGCCGTG GAAACGCCTT TTGTTCCAGT CCTTTGTCAG TCTCCCTGTC 
TGTGTCTCCT GTCTGTGTTT TGTCTTTGTG GTCATGCTTG CCTGCTTTGA GCTTCAGGAG 
TTTATACTTA GTATCAAGGA GCTCCCACGG GTTGTACGTT TCTTTCCCAA GATTCTCCTG 
GCCGTTATAG TTAGCTCATG TGACGAAGTT TACAGGAAGA TTGCCTACTG GCTAAATGAT 
ATGGAAAATT ATCGTTTACA GAGTGCCTAC GAGAAACATC TCATCATTAA AATTGTTTTA 
TTCCAGTTTG TAAATTCCTA CCTGAGTCTT TTCTACATTG GTTTCTACCT CAAGGACATG 
GAGCGACTGA AAGAGCTGTT CCTCGTTTTG TCTCTCCCCC ATAGCTTCAC TCGTCAGGTT 
AAAGATGCCC TCCTCCCTTT CCTCTCACTC CAGTTTCACC TGTTCCTCAT CGTCCTCAGG 
GCACTTCTCA GATCAGCCTG GTGCCTCTTC AAATCTAAAA TGCTTGCCAC GCTCCTGGTC 
ATTCGCCAGT TCCTGCAGAA TGTGAAAGAA GTGTCCCAAC CTCATCTATA CCAGAAACTA 
CAACGGGGAG AGCTGACTCC ACGGTCAGTC TGGCTCTTTG TTCGCTATGC CCTGAAGAGG 
TTGTTTCAAA AATATTCTCT CCCTCAGCAC CCATTAAGGA CTCCTGGGAA CCCAATGGAG 
ACGGGTGCAG GTGGGCGCAA GAAGTGCCTC AATGGAGGGT GTGGTGTACC AGAAGAGGAG 
GAGGGAGTGG TCTCTGAAGA TGAGGAAGAG GAGGAGGAAG AAAGTATTAT GGATTGTGGA 
CTCAAATTAA AAAGGGTTAG TTTTTTAGAG AAAGGGGAGA GGAGAGAGGA TGTTGGTCTA 
GTTCCTCAGC AAGAAGATGA GGGTGCCTTT TTACAGGAGG GTAGTCCTAC TCTTATGGAA 
AGAGGTAGCG ACCCAGCCTC AGTTTTTCAG CTGGAAGATG AAGAGGATGA GGAACAAGAA 
GGAGCAAGTG GTGGAGTACT GCAGGAGGGT TTAGAGCAAG AGTCCGGGGT AAAGCTGAGG 
CAGCACAGAA GGAGGAGAAA GAGGGTAATT CTGGAGGAAG AACAAAGTAG AGAAGATGAG 
GAGGATGATA CAGAGATGAA GCAGAAAAGA GAATCATGGA TTGACCCTCC AGAGGACAAT 
TATCCAACTA AGCTTTCTCA AGCTGAGGTG GAGAGTTGTA TGAAGAAATA TGAGGACACT 
TTCAACGATT ACCAAGAGAT GTTTGTGCAG TTTGGGTATG TGGTCCTCTT TTCCTCGGCC 
TTCCCCTTGG CTGCTGTCTG TGCCCTGATC AATAACATAA TCGAGATCCG CAGTGATGCC 
TTTAAGCTGT GCACTGGGCT TCAGAGACCA TTTGGGCGTC GTGTAGATGG CATAGGACAG 
TGGCAAAATG TAATGGAGGC GATGGGGGTT ATAGCCATTA TTGTAAACTG CTACTTAATG 
GGCCAGTGTG GACAGCTTCA AAGACTGCTA CCCTGGCTGG GGCCCGAAAC AACAATCATA 
TTTATTGTGG TGCTTGAGCA TTTTGCTTTG CTCCTGAAGT ATATAATACA CGTTGTGATC 
CCAGACATCC CTGGATGGGT GGCAGAGGAG ATGGCAAAAC TGGAATATCA AAGAAGAGAA 
GCTTTTAAGA AACATGAGCG ACAAGCTCAG CATCGCTACC AGCAGCAACA GCGCAGGAAG 
CGTGAGGAGG AGGAGAGACA GCGCCATGCT GATTACCATG CGAGGAAAGA GCGAGAATCT 
CGTGGAGGGG ACGATGGAAA AAACTCTGGA CAAGATTCCC AGAATCATGA AAAGAACTCA 
AGCAAAACCA AGCAAGGTGG CCCCCCTGGA GGGAGCAGCC ATGATAAGCC GAAAAGACCC 
AGCTCCCTCT TGGCCAGCAA TAATGTGATG AAACTTAAAC AAATTATTCC CCTGCAAAGC 
AAGTTCTTAT CAGGGACCAA TGCCAAATCT CCACAGTCCC CTCCAGCGAC AGACACCAAA 
ATCCCCAGCT TTCTGAGCTT CAAATTTCTA AAATCCCCTG AAACAAAAAG AGAATCCTCC 
CAAGAGAGAG CCCATTCTCC TACAAAGCCT TTCCACCCAG GCAAGCTCTT TAACTTTGGC 
GGAAAGGGTG AGGGACAGGT GGGTGGTAAT GGTATTGGTC CACCCATATC AACACAGCCA 
AGAGGAGCAA CAGCTGGGAC TGCCTCCTCT GATGGTACAG TGCCAGGTAA GTCCCAGCTA 
AATGGGATTT CAGAGGATAG ACCCACGGAG GAGGGTGAAG AACAAACCAA GGCACAAAAA 
CAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ano8 ( XM_004911017.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911074.1 Xenopus tropicalis anoctamin 8 (ano8), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa04340
Clone ID Related Accession (Same CDS sequence)	XM_004911016.3 , XM_004911017.3 , XM_004911016.4 , XM_004911017.4
Accession Version	XM_004911017.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	anoctamin-8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004911017.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGACA CAGAGCCGGA CAGAGGCAAG AAAGCCCAGC CGGAAGGGGA GCGAGCAGAA 
CCGGCTGGCA GCCAGCATGG CACGACTGGA GTACTGGATA AACTTTTTGG GAAGCGTCTT 
CTGCAAGCTG GTCGATATAT CACATCCCAC AAATCCTGGA TGAGGACTGT ACCCACAGAA 
AACTGTGACG TAGTTATGAC GTTCTCTGAT AACACGGATG ACCACACTTT GCTGTGGCTA 
CTGAATCATG TACGCCTGGG GTTACCTGAA CTTATTATGC AGATTCGACA CCACAGACAC 
ACTGGGATCT ACGCCTTCTT TGTAACTGCC ACATATGAAA ATTTGCTCCG CGGTGCGGAG 
GAGATTGGTC TTCGAAAACC AGTTAAAGCA GAGTTTGGAG GTGGAACACG AGTCTTCTCA 
TGCGAAGAGG ATTATATTTA TCAGAACATT GAAAATGAAC TCATTTTTTT CACATCTCAG 
GAACGTCAAA GCATCATCCG CTACTGGATG GAAAACCTCC GAGCCAAACA AGGCGAATCC 
CTGCACAATA TCCACTTCCT TGAGGGCCAG CCAATCATCC CAGAGTTGAC AGCCCGTGGG 
GTCATCCAGC AAGTGTTTCC CATCCATGAA CAAAGAATAT TAAGCCGACT TATGAGGTCC 
TGGGTCCAAG CAATCTGCGA AAAACAACCC CTTGATGAAA TCTGTGACTA TTTTGGGGTG 
AAAGTTGCCA TGTATTTTTC CTGGATGGGT TTCTACACCT CCTCCCTTGT CTATCCAGCT 
GTCTTTGGCA TGATGCTGTG GATGATCACT GAGTCTGACC AGACAAGCCG TGACATTTGC 
TGTGTTGTCT TTGCCCTTTT CAATGTTGTT TGGGCTACAT TATTCTTAGA GGGATGGAAA 
AGGAGAGGGG CAGAGTTGGC GTACAAGTGG GGGACACTGG ACACCCCTGC TGAATTTATT 
GAGGAGCCAA GGCCCCAATT CAGGGGTGTC AAGAGGATCA GTCCAGTCAC TAACTGCGAA 
GAGTTTTATT ACCCGCCGTG GAAACGCCTT TTGTTCCAGT CCTTTGTCAG TCTCCCTGTC 
TGTGTCTCCT GTCTGTGTTT TGTCTTTGTG GTCATGCTTG CCTGCTTTGA GCTTCAGGAG 
TTTATACTTA GTATCAAGGA GCTCCCACGG GTTGTACGTT TCTTTCCCAA GATTCTCCTG 
GCCGTTATAG TTAGCTCATG TGACGAAGTT TACAGGAAGA TTGCCTACTG GCTAAATGAT 
ATGGAAAATT ATCGTTTACA GAGTGCCTAC GAGAAACATC TCATCATTAA AATTGTTTTA 
TTCCAGTTTG TAAATTCCTA CCTGAGTCTT TTCTACATTG GTTTCTACCT CAAGGACATG 
GAGCGACTGA AAGAGCTGTT CCTCGTTTTG TCTCTCCCCC ATAGCTTCAC TCGTCAGGTT 
AAAGATGCCC TCCTCCCTTT CCTCTCACTC CAGTTTCACC TGTTCCTCAT CGTCCTCAGG 
GCACTTCTCA GATCAGCCTG GTGCCTCTTC AAATCTAAAA TGCTTGCCAC GCTCCTGGTC 
ATTCGCCAGT TCCTGCAGAA TGTGAAAGAA GTGTCCCAAC CTCATCTATA CCAGAAACTA 
CAACGGGGAG AGCTGACTCC ACGGTCAGTC TGGCTCTTTG TTCGCTATGC CCTGAAGAGG 
TTGTTTCAAA AATATTCTCT CCCTCAGCAC CCATTAAGGA CTCCTGGGAA CCCAATGGAG 
ACGGGTGCAG GTGGGCGCAA GAAGTGCCTC AATGGAGGGT GTGGTGTACC AGAAGAGGAG 
GAGGGAGTGG TCTCTGAAGA TGAGGAAGAG GAGGAGGAAG AAAGTATTAT GGATTGTGGA 
CTCAAATTAA AAAGGGTTAG TTTTTTAGAG AAAGGGGAGA GGAGAGAGGA TGTTGGTCTA 
GTTCCTCAGC AAGAAGATGA GGGTGCCTTT TTACAGGAGG GTAGTCCTAC TCTTATGGAA 
AGAGGTAGCG ACCCAGCCTC AGTTTTTCAG CTGGAAGATG AAGAGGATGA GGAACAAGAA 
GGAGCAAGTG GTGGAGTACT GCAGGAGGGT TTAGAGCAAG AGTCCGGGGT AAAGCTGAGG 
CAGCACAGAA GGAGGAGAAA GAGGGTAATT CTGGAGGAAG AACAAAGTAG AGAAGATGAG 
GAGGATGATA CAGAGATGAA GCAGAAAAGA GAATCATGGA TTGACCCTCC AGAGGACAAT 
TATCCAACTA AGCTTTCTCA AGCTGAGGTG GAGAGTTGTA TGAAGAAATA TGAGGACACT 
TTCAACGATT ACCAAGAGAT GTTTGTGCAG TTTGGGTATG TGGTCCTCTT TTCCTCGGCC 
TTCCCCTTGG CTGCTGTCTG TGCCCTGATC AATAACATAA TCGAGATCCG CAGTGATGCC 
TTTAAGCTGT GCACTGGGCT TCAGAGACCA TTTGGGCGTC GTGTAGATGG CATAGGACAG 
TGGCAAAATG TAATGGAGGC GATGGGGGTT ATAGCCATTA TTGTAAACTG CTACTTAATG 
GGCCAGTGTG GACAGCTTCA AAGACTGCTA CCCTGGCTGG GGCCCGAAAC AACAATCATA 
TTTATTGTGG TGCTTGAGCA TTTTGCTTTG CTCCTGAAGT ATATAATACA CGTTGTGATC 
CCAGACATCC CTGGATGGGT GGCAGAGGAG ATGGCAAAAC TGGAATATCA AAGAAGAGAA 
GCTTTTAAGA AACATGAGCG ACAAGCTCAG CATCGCTACC AGCAGCAACA GCGCAGGAAG 
CGTGAGGAGG AGGAGAGACA GCGCCATGCT GATTACCATG CGAGGAAAGA GCGAGAATCT 
CGTGGAGGGG ACGATGGAAA AAACTCTGGA CAAGATTCCC AGAATCATGA AAAGAACTCA 
AGCAAAACCA AGCAAGGTGG CCCCCCTGGA GGGAGCAGCC ATGATAAGCC GAAAAGACCC 
AGCTCCCTCT TGGCCAGCAA TAATGTGATG AAACTTAAAC AAATTATTCC CCTGCAAAGC 
AAGTTCTTAT CAGGGACCAA TGCCAAATCT CCACAGTCCC CTCCAGCGAC AGACACCAAA 
ATCCCCAGCT TTCTGAGCTT CAAATTTCTA AAATCCCCTG AAACAAAAAG AGAATCCTCC 
CAAGAGAGAG CCCATTCTCC TACAAAGCCT TTCCACCCAG GCAAGCTCTT TAACTTTGGC 
GGAAAGGGTG AGGGACAGGT GGGTGGTAAT GGTATTGGTC CACCCATATC AACACAGCCA 
AGAGGAGCAA CAGCTGGGAC TGCCTCCTCT GATGGTACAG TGCCAGGTAA GTCCCAGCTA 
AATGGGATTT CAGAGGATAG ACCCACGGAG GAGGGTGAAG AACAAACCAA GGCACAAAAA 
CAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911074.1
CDS	271..3636
Translation	MPDTEPDRGKKAQPEGERAEPAGSQHGTTGVLDKLFGKRLLQAGRYITSHKSWMRTVPTENCDVVMTFSDNTDDHTLLWLLNHVRLGLPELIMQIRHHRHTGIYAFFVTATYENLLRGAEEIGLRKPVKAEFGGGTRVFSCEEDYIYQNIENELIFFTSQERQSIIRYWMENLRAKQGESLHNIHFLEGQPIIPELTARGVIQQVFPIHEQRILSRLMRSWVQAICEKQPLDEICDYFGVKVAMYFSWMGFYTSSLVYPAVFGMMLWMITESDQTSRDICCVVFALFNVVWATLFLEGWKRRGAELAYKWGTLDTPAEFIEEPRPQFRGVKRISPVTNCEEFYYPPWKRLLFQSFVSLPVCVSCLCFVFVVMLACFELQEFILSIKELPRVVRFFPKILLAVIVSSCDEVYRKIAYWLNDMENYRLQSAYEKHLIIKIVLFQFVNSYLSLFYIGFYLKDMERLKELFLVLSLPHSFTRQVKDALLPFLSLQFHLFLIVLRALLRSAWCLFKSKMLATLLVIRQFLQNVKEVSQPHLYQKLQRGELTPRSVWLFVRYALKRLFQKYSLPQHPLRTPGNPMETGAGGRKKCLNGGCGVPEEEEGVVSEDEEEEEEESIMDCGLKLKRVSFLEKGERREDVGLVPQQEDEGAFLQEGSPTLMERGSDPASVFQLEDEEDEEQEGASGGVLQEGLEQESGVKLRQHRRRRKRVILEEEQSREDEEDDTEMKQKRESWIDPPEDNYPTKLSQAEVESCMKKYEDTFNDYQEMFVQFGYVVLFSSAFPLAAVCALINNIIEIRSDAFKLCTGLQRPFGRRVDGIGQWQNVMEAMGVIAIIVNCYLMGQCGQLQRLLPWLGPETTIIFIVVLEHFALLLKYIIHVVIPDIPGWVAEEMAKLEYQRREAFKKHERQAQHRYQQQQRRKREEEERQRHADYHARKERESRGGDDGKNSGQDSQNHEKNSSKTKQGGPPGGSSHDKPKRPSSLLASNNVMKLKQIIPLQSKFLSGTNAKSPQSPPATDTKIPSFLSFKFLKSPETKRESSQERAHSPTKPFHPGKLFNFGGKGEGQVGGNGIGPPISTQPRGATAGTASSDGTVPGKSQLNGISEDRPTEEGEEQTKAQKQ

Target ORF information:

RefSeq Version	XM_004911017.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis anoctamin 8 (ano8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911017.4

ORF Insert Sequence:

ATGCCGGACA CAGAGCCGGA CAGAGGCAAG AAAGCCCAGC CGGAAGGGGA GCGAGCAGAA 
CCGGCTGGCA GCCAGCATGG CACGACTGGA GTACTGGATA AACTTTTTGG GAAGCGTCTT 
CTGCAAGCTG GTCGATATAT CACATCCCAC AAATCCTGGA TGAGGACTGT ACCCACAGAA 
AACTGTGACG TAGTTATGAC GTTCTCTGAT AACACGGATG ACCACACTTT GCTGTGGCTA 
CTGAATCATG TACGCCTGGG GTTACCTGAA CTTATTATGC AGATTCGACA CCACAGACAC 
ACTGGGATCT ACGCCTTCTT TGTAACTGCC ACATATGAAA ATTTGCTCCG CGGTGCGGAG 
GAGATTGGTC TTCGAAAACC AGTTAAAGCA GAGTTTGGAG GTGGAACACG AGTCTTCTCA 
TGCGAAGAGG ATTATATTTA TCAGAACATT GAAAATGAAC TCATTTTTTT CACATCTCAG 
GAACGTCAAA GCATCATCCG CTACTGGATG GAAAACCTCC GAGCCAAACA AGGCGAATCC 
CTGCACAATA TCCACTTCCT TGAGGGCCAG CCAATCATCC CAGAGTTGAC AGCCCGTGGG 
GTCATCCAGC AAGTGTTTCC CATCCATGAA CAAAGAATAT TAAGCCGACT TATGAGGTCC 
TGGGTCCAAG CAATCTGCGA AAAACAACCC CTTGATGAAA TCTGTGACTA TTTTGGGGTG 
AAAGTTGCCA TGTATTTTTC CTGGATGGGT TTCTACACCT CCTCCCTTGT CTATCCAGCT 
GTCTTTGGCA TGATGCTGTG GATGATCACT GAGTCTGACC AGACAAGCCG TGACATTTGC 
TGTGTTGTCT TTGCCCTTTT CAATGTTGTT TGGGCTACAT TATTCTTAGA GGGATGGAAA 
AGGAGAGGGG CAGAGTTGGC GTACAAGTGG GGGACACTGG ACACCCCTGC TGAATTTATT 
GAGGAGCCAA GGCCCCAATT CAGGGGTGTC AAGAGGATCA GTCCAGTCAC TAACTGCGAA 
GAGTTTTATT ACCCGCCGTG GAAACGCCTT TTGTTCCAGT CCTTTGTCAG TCTCCCTGTC 
TGTGTCTCCT GTCTGTGTTT TGTCTTTGTG GTCATGCTTG CCTGCTTTGA GCTTCAGGAG 
TTTATACTTA GTATCAAGGA GCTCCCACGG GTTGTACGTT TCTTTCCCAA GATTCTCCTG 
GCCGTTATAG TTAGCTCATG TGACGAAGTT TACAGGAAGA TTGCCTACTG GCTAAATGAT 
ATGGAAAATT ATCGTTTACA GAGTGCCTAC GAGAAACATC TCATCATTAA AATTGTTTTA 
TTCCAGTTTG TAAATTCCTA CCTGAGTCTT TTCTACATTG GTTTCTACCT CAAGGACATG 
GAGCGACTGA AAGAGCTGTT CCTCGTTTTG TCTCTCCCCC ATAGCTTCAC TCGTCAGGTT 
AAAGATGCCC TCCTCCCTTT CCTCTCACTC CAGTTTCACC TGTTCCTCAT CGTCCTCAGG 
GCACTTCTCA GATCAGCCTG GTGCCTCTTC AAATCTAAAA TGCTTGCCAC GCTCCTGGTC 
ATTCGCCAGT TCCTGCAGAA TGTGAAAGAA GTGTCCCAAC CTCATCTATA CCAGAAACTA 
CAACGGGGAG AGCTGACTCC ACGGTCAGTC TGGCTCTTTG TTCGCTATGC CCTGAAGAGG 
TTGTTTCAAA AATATTCTCT CCCTCAGCAC CCATTAAGGA CTCCTGGGAA CCCAATGGAG 
ACGGGTGCAG GTGGGCGCAA GAAGTGCCTC AATGGAGGGT GTGGTGTACC AGAAGAGGAG 
GAGGGAGTGG TCTCTGAAGA TGAGGAAGAG GAGGAGGAAG AAAGTATTAT GGATTGTGGA 
CTCAAATTAA AAAGGGTTAG TTTTTTAGAG AAAGGGGAGA GGAGAGAGGA TGTTGGTCTA 
GTTCCTCAGC AAGAAGATGA GGGTGCCTTT TTACAGGAGG GTAGTCCTAC TCTTATGGAA 
AGAGGTAGCG ACCCAGCCTC AGTTTTTCAG CTGGAAGATG AAGAGGATGA GGAACAAGAA 
GGAGCAAGTG GTGGAGTACT GCAGGAGGGT TTAGAGCAAG AGTCCGGGGT AAAGCTGAGG 
CAGCACAGAA GGAGGAGAAA GAGGGTAATT CTGGAGGAAG AACAAAGTAG AGAAGATGAG 
GAGGATGATA CAGAGATGAA GCAGAAAAGA GAATCATGGA TTGACCCTCC AGAGGACAAT 
TATCCAACTA AGCTTTCTCA AGCTGAGGTG GAGAGTTGTA TGAAGAAATA TGAGGACACT 
TTCAACGATT ACCAAGAGAT GTTTGTGCAG TTTGGGTATG TGGTCCTCTT TTCCTCGGCC 
TTCCCCTTGG CTGCTGTCTG TGCCCTGATC AATAACATAA TCGAGATCCG CAGTGATGCC 
TTTAAGCTGT GCACTGGGCT TCAGAGACCA TTTGGGCGTC GTGTAGATGG CATAGGACAG 
TGGCAAAATG TAATGGAGGC GATGGGGGTT ATAGCCATTA TTGTAAACTG CTACTTAATG 
GGCCAGTGTG GACAGCTTCA AAGACTGCTA CCCTGGCTGG GGCCCGAAAC AACAATCATA 
TTTATTGTGG TGCTTGAGCA TTTTGCTTTG CTCCTGAAGT ATATAATACA CGTTGTGATC 
CCAGACATCC CTGGATGGGT GGCAGAGGAG ATGGCAAAAC TGGAATATCA AAGAAGAGAA 
GCTTTTAAGA AACATGAGCG ACAAGCTCAG CATCGCTACC AGCAGCAACA GCGCAGGAAG 
CGTGAGGAGG AGGAGAGACA GCGCCATGCT GATTACCATG CGAGGAAAGA GCGAGAATCT 
CGTGGAGGGG ACGATGGAAA AAACTCTGGA CAAGATTCCC AGAATCATGA AAAGAACTCA 
AGCAAAACCA AGCAAGGTGG CCCCCCTGGA GGGAGCAGCC ATGATAAGCC GAAAAGACCC 
AGCTCCCTCT TGGCCAGCAA TAATGTGATG AAACTTAAAC AAATTATTCC CCTGCAAAGC 
AAGTTCTTAT CAGGGACCAA TGCCAAATCT CCACAGTCCC CTCCAGCGAC AGACACCAAA 
ATCCCCAGCT TTCTGAGCTT CAAATTTCTA AAATCCCCTG AAACAAAAAG AGAATCCTCC 
CAAGAGAGAG CCCATTCTCC TACAAAGCCT TTCCACCCAG GCAAGCTCTT TAACTTTGGC 
GGAAAGGGTG AGGGACAGGT GGGTGGTAAT GGTATTGGTC CACCCATATC AACACAGCCA 
AGAGGAGCAA CAGCTGGGAC TGCCTCCTCT GATGGTACAG TGCCAGGTAA GTCCCAGCTA 
AATGGGATTT CAGAGGATAG ACCCACGGAG GAGGGTGAAG AACAAACCAA GGCACAAAAA 
CAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ano8 ( XM_002936335.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002936381.1 Xenopus tropicalis anoctamin 8 (ano8), transcript variant X3, mRNA.

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CloneID	OXa04341
Clone ID Related Accession (Same CDS sequence)	XM_002936335.5 , XM_002936335.4
Accession Version	XM_002936335.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	anoctamin-8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002936335.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGACA CAGAGCCGGA CAGAGGCAAG AAAGCCCAGC CGGAAGGGGA GCGAGCAGAA 
CCGGCTGGCA GCCAGCATGG CACGACTGGA GTACTGGATA AACTTTTTGG GAAGCGTCTT 
CTGCAAGCTG GTCGATATAT CACATCCCAC AAATCCTGGA TGAGGACTGT ACCCACAGAA 
AACTGTGACG TAGTTATGAC GTTCTCTGAT AACACGGATG ACCACACTTT GCTGTGGCTA 
CTGAATCATG TACGCCTGGG GTTACCTGAA CTTATTATGC AGATTCGACA CCACAGACAC 
ACTGGGATCT ACGCCTTCTT TGTAACTGCC ACATATGAAA ATTTGCTCCG CGGTGCGGAG 
GAGATTGGTC TTCGAAAACC AGTTAAAGCA GAGTTTGGAG GTGGAACACG AGTCTTCTCA 
TGCGAAGAGG ATTATATTTA TCAGAACATT GAAAATGAAC TCATTTTTTT CACATCTCAG 
GAACGTCAAA GCATCATCCG CTACTGGATG GAAAACCTCC GAGCCAAACA AGGCGAATCC 
CTGCACAATA TCCACTTCCT TGAGGGCCAG CCAATCATCC CAGAGTTGAC AGCCCGTGGG 
GTCATCCAGC AAGTGTTTCC CATCCATGAA CAAAGAATAT TAAGCCGACT TATGAGGTCC 
TGGGTCCAAG CAATCTGCGA AAAACAACCC CTTGATGAAA TCTGTGACTA TTTTGGGGTG 
AAAGTTGCCA TGTATTTTTC CTGGATGGGT TTCTACACCT CCTCCCTTGT CTATCCAGCT 
GTCTTTGGCA TGATGCTGTG GATGATCACT GAGTCTGACC AGACAAGCCG TGACATTTGC 
TGTGTTGTCT TTGCCCTTTT CAATGTTGTT TGGGCTACAT TATTCTTAGA GGGATGGAAA 
AGGAGAGGGG CAGAGTTGGC GTACAAGTGG GGGACACTGG ACACCCCTGC TGAATTTATT 
GAGGAGCCAA GGCCCCAATT CAGGGGTGTC AAGAGGATCA GTCCAGTCAC TAACTGCGAA 
GAGTTTTATT ACCCGCCGTG GAAACGCCTT TTGTTCCAGT CCTTTGTCAG TCTCCCTGTC 
TGTGTCTCCT GTCTGTGTTT TGTCTTTGTG GTCATGCTTG CCTGCTTTGA GCTTCAGGAG 
TTTATACTTA GTATCAAGGA GCTCCCACGG GTTGTACGTT TCTTTCCCAA GATTCTCCTG 
GCCGTTATAG TTAGCTCATG TGACGAAGTT TACAGGAAGA TTGCCTACTG GCTAAATGAT 
ATGGAAAATT ATCGTTTACA GAGTGCCTAC GAGAAACATC TCATCATTAA AATTGTTTTA 
TTCCAGTTTG TAAATTCCTA CCTGAGTCTT TTCTACATTG GTTTCTACCT CAAGGACATG 
GAGCGACTGA AAGAGATGCT TGCCACGCTC CTGGTCATTC GCCAGTTCCT GCAGAATGTG 
AAAGAAGTGT CCCAACCTCA TCTATACCAG AAACTACAAC GGGGAGAGCT GACTCCACGG 
TCAGTCTGGC TCTTTGTTCG CTATGCCCTG AAGAGGTTGT TTCAAAAATA TTCTCTCCCT 
CAGCACCCAT TAAGGACTCC TGGGAACCCA ATGGAGACGG GTGCAGGTGG GCGCAAGAAG 
TGCCTCAATG GAGGGTGTGG TGTACCAGAA GAGGAGGAGG GAGTGGTCTC TGAAGATGAG 
GAAGAGGAGG AGGAAGAAAG TATTATGGAT TGTGGACTCA AATTAAAAAG GGTTAGTTTT 
TTAGAGAAAG GGGAGAGGAG AGAGGATGTT GGTCTAGTTC CTCAGCAAGA AGATGAGGGT 
GCCTTTTTAC AGGAGGGTAG TCCTACTCTT ATGGAAAGAG GTAGCGACCC AGCCTCAGTT 
TTTCAGCTGG AAGATGAAGA GGATGAGGAA CAAGAAGGAG CAAGTGGTGG AGTACTGCAG 
GAGGGTTTAG AGCAAGAGTC CGGGGTAAAG CTGAGGCAGC ACAGAAGGAG GAGAAAGAGG 
GTAATTCTGG AGGAAGAACA AAGTAGAGAA GATGAGGAGG ATGATACAGA GATGAAGCAG 
AAAAGAGAAT CATGGATTGA CCCTCCAGAG GACAATTATC CAACTAAGCT TTCTCAAGCT 
GAGGTGGAGA GTTGTATGAA GAAATATGAG GACACTTTCA ACGATTACCA AGAGATGTTT 
GTGCAGTTTG GGTATGTGGT CCTCTTTTCC TCGGCCTTCC CCTTGGCTGC TGTCTGTGCC 
CTGATCAATA ACATAATCGA GATCCGCAGT GATGCCTTTA AGCTGTGCAC TGGGCTTCAG 
AGACCATTTG GGCGTCGTGT AGATGGCATA GGACAGTGGC AAAATGTAAT GGAGGCGATG 
GGGGTTATAG CCATTATTGT AAACTGCTAC TTAATGGGCC AGTGTGGACA GCTTCAAAGA 
CTGCTACCCT GGCTGGGGCC CGAAACAACA ATCATATTTA TTGTGGTGCT TGAGCATTTT 
GCTTTGCTCC TGAAGTATAT AATACACGTT GTGATCCCAG ACATCCCTGG ATGGGTGGCA 
GAGGAGATGG CAAAACTGGA ATATCAAAGA AGAGAAGCTT TTAAGAAACA TGAGCGACAA 
GCTCAGCATC GCTACCAGCA GCAACAGCGC AGGAAGCGTG AGGAGGAGGA GAGACAGCGC 
CATGCTGATT ACCATGCGAG GAAAGAGCGA GAATCTCGTG GAGGGGACGA TGGAAAAAAC 
TCTGGACAAG ATTCCCAGAA TCATGAAAAG AACTCAAGCA AAACCAAGCA AGGTGGCCCC 
CCTGGAGGGA GCAGCCATGA TAAGCCGAAA AGACCCAGCT CCCTCTTGGC CAGCAATAAT 
GTGATGAAAC TTAAACAAAT TATTCCCCTG CAAAGCAAGT TCTTATCAGG GACCAATGCC 
AAATCTCCAC AGTCCCCTCC AGCGACAGAC ACCAAAATCC CCAGCTTTCT GAGCTTCAAA 
TTTCTAAAAT CCCCTGAAAC AAAAAGAGAA TCCTCCCAAG AGAGAGCCCA TTCTCCTACA 
AAGCCTTTCC ACCCAGGCAA GCTCTTTAAC TTTGGCGGAA AGGGTGAGGG ACAGGTGGGT 
GGTAATGGTA TTGGTCCACC CATATCAACA CAGCCAAGAG GAGCAACAGC TGGGACTGCC 
TCCTCTGATG GTACAGTGCC AGGTAAGTCC CAGCTAAATG GGATTTCAGA GGATAGACCC 
ACGGAGGAGG GTGAAGAACA AACCAAGGCA CAAAAACAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002936381.1
CDS	515..3736
Translation	MPDTEPDRGKKAQPEGERAEPAGSQHGTTGVLDKLFGKRLLQAGRYITSHKSWMRTVPTENCDVVMTFSDNTDDHTLLWLLNHVRLGLPELIMQIRHHRHTGIYAFFVTATYENLLRGAEEIGLRKPVKAEFGGGTRVFSCEEDYIYQNIENELIFFTSQERQSIIRYWMENLRAKQGESLHNIHFLEGQPIIPELTARGVIQQVFPIHEQRILSRLMRSWVQAICEKQPLDEICDYFGVKVAMYFSWMGFYTSSLVYPAVFGMMLWMITESDQTSRDICCVVFALFNVVWATLFLEGWKRRGAELAYKWGTLDTPAEFIEEPRPQFRGVKRISPVTNCEEFYYPPWKRLLFQSFVSLPVCVSCLCFVFVVMLACFELQEFILSIKELPRVVRFFPKILLAVIVSSCDEVYRKIAYWLNDMENYRLQSAYEKHLIIKIVLFQFVNSYLSLFYIGFYLKDMERLKEMLATLLVIRQFLQNVKEVSQPHLYQKLQRGELTPRSVWLFVRYALKRLFQKYSLPQHPLRTPGNPMETGAGGRKKCLNGGCGVPEEEEGVVSEDEEEEEEESIMDCGLKLKRVSFLEKGERREDVGLVPQQEDEGAFLQEGSPTLMERGSDPASVFQLEDEEDEEQEGASGGVLQEGLEQESGVKLRQHRRRRKRVILEEEQSREDEEDDTEMKQKRESWIDPPEDNYPTKLSQAEVESCMKKYEDTFNDYQEMFVQFGYVVLFSSAFPLAAVCALINNIIEIRSDAFKLCTGLQRPFGRRVDGIGQWQNVMEAMGVIAIIVNCYLMGQCGQLQRLLPWLGPETTIIFIVVLEHFALLLKYIIHVVIPDIPGWVAEEMAKLEYQRREAFKKHERQAQHRYQQQQRRKREEEERQRHADYHARKERESRGGDDGKNSGQDSQNHEKNSSKTKQGGPPGGSSHDKPKRPSSLLASNNVMKLKQIIPLQSKFLSGTNAKSPQSPPATDTKIPSFLSFKFLKSPETKRESSQERAHSPTKPFHPGKLFNFGGKGEGQVGGNGIGPPISTQPRGATAGTASSDGTVPGKSQLNGISEDRPTEEGEEQTKAQKQ

Target ORF information:

RefSeq Version	XM_002936335.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis anoctamin 8 (ano8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002936335.5

ORF Insert Sequence:

ATGCCGGACA CAGAGCCGGA CAGAGGCAAG AAAGCCCAGC CGGAAGGGGA GCGAGCAGAA 
CCGGCTGGCA GCCAGCATGG CACGACTGGA GTACTGGATA AACTTTTTGG GAAGCGTCTT 
CTGCAAGCTG GTCGATATAT CACATCCCAC AAATCCTGGA TGAGGACTGT ACCCACAGAA 
AACTGTGACG TAGTTATGAC GTTCTCTGAT AACACGGATG ACCACACTTT GCTGTGGCTA 
CTGAATCATG TACGCCTGGG GTTACCTGAA CTTATTATGC AGATTCGACA CCACAGACAC 
ACTGGGATCT ACGCCTTCTT TGTAACTGCC ACATATGAAA ATTTGCTCCG CGGTGCGGAG 
GAGATTGGTC TTCGAAAACC AGTTAAAGCA GAGTTTGGAG GTGGAACACG AGTCTTCTCA 
TGCGAAGAGG ATTATATTTA TCAGAACATT GAAAATGAAC TCATTTTTTT CACATCTCAG 
GAACGTCAAA GCATCATCCG CTACTGGATG GAAAACCTCC GAGCCAAACA AGGCGAATCC 
CTGCACAATA TCCACTTCCT TGAGGGCCAG CCAATCATCC CAGAGTTGAC AGCCCGTGGG 
GTCATCCAGC AAGTGTTTCC CATCCATGAA CAAAGAATAT TAAGCCGACT TATGAGGTCC 
TGGGTCCAAG CAATCTGCGA AAAACAACCC CTTGATGAAA TCTGTGACTA TTTTGGGGTG 
AAAGTTGCCA TGTATTTTTC CTGGATGGGT TTCTACACCT CCTCCCTTGT CTATCCAGCT 
GTCTTTGGCA TGATGCTGTG GATGATCACT GAGTCTGACC AGACAAGCCG TGACATTTGC 
TGTGTTGTCT TTGCCCTTTT CAATGTTGTT TGGGCTACAT TATTCTTAGA GGGATGGAAA 
AGGAGAGGGG CAGAGTTGGC GTACAAGTGG GGGACACTGG ACACCCCTGC TGAATTTATT 
GAGGAGCCAA GGCCCCAATT CAGGGGTGTC AAGAGGATCA GTCCAGTCAC TAACTGCGAA 
GAGTTTTATT ACCCGCCGTG GAAACGCCTT TTGTTCCAGT CCTTTGTCAG TCTCCCTGTC 
TGTGTCTCCT GTCTGTGTTT TGTCTTTGTG GTCATGCTTG CCTGCTTTGA GCTTCAGGAG 
TTTATACTTA GTATCAAGGA GCTCCCACGG GTTGTACGTT TCTTTCCCAA GATTCTCCTG 
GCCGTTATAG TTAGCTCATG TGACGAAGTT TACAGGAAGA TTGCCTACTG GCTAAATGAT 
ATGGAAAATT ATCGTTTACA GAGTGCCTAC GAGAAACATC TCATCATTAA AATTGTTTTA 
TTCCAGTTTG TAAATTCCTA CCTGAGTCTT TTCTACATTG GTTTCTACCT CAAGGACATG 
GAGCGACTGA AAGAGATGCT TGCCACGCTC CTGGTCATTC GCCAGTTCCT GCAGAATGTG 
AAAGAAGTGT CCCAACCTCA TCTATACCAG AAACTACAAC GGGGAGAGCT GACTCCACGG 
TCAGTCTGGC TCTTTGTTCG CTATGCCCTG AAGAGGTTGT TTCAAAAATA TTCTCTCCCT 
CAGCACCCAT TAAGGACTCC TGGGAACCCA ATGGAGACGG GTGCAGGTGG GCGCAAGAAG 
TGCCTCAATG GAGGGTGTGG TGTACCAGAA GAGGAGGAGG GAGTGGTCTC TGAAGATGAG 
GAAGAGGAGG AGGAAGAAAG TATTATGGAT TGTGGACTCA AATTAAAAAG GGTTAGTTTT 
TTAGAGAAAG GGGAGAGGAG AGAGGATGTT GGTCTAGTTC CTCAGCAAGA AGATGAGGGT 
GCCTTTTTAC AGGAGGGTAG TCCTACTCTT ATGGAAAGAG GTAGCGACCC AGCCTCAGTT 
TTTCAGCTGG AAGATGAAGA GGATGAGGAA CAAGAAGGAG CAAGTGGTGG AGTACTGCAG 
GAGGGTTTAG AGCAAGAGTC CGGGGTAAAG CTGAGGCAGC ACAGAAGGAG GAGAAAGAGG 
GTAATTCTGG AGGAAGAACA AAGTAGAGAA GATGAGGAGG ATGATACAGA GATGAAGCAG 
AAAAGAGAAT CATGGATTGA CCCTCCAGAG GACAATTATC CAACTAAGCT TTCTCAAGCT 
GAGGTGGAGA GTTGTATGAA GAAATATGAG GACACTTTCA ACGATTACCA AGAGATGTTT 
GTGCAGTTTG GGTATGTGGT CCTCTTTTCC TCGGCCTTCC CCTTGGCTGC TGTCTGTGCC 
CTGATCAATA ACATAATCGA GATCCGCAGT GATGCCTTTA AGCTGTGCAC TGGGCTTCAG 
AGACCATTTG GGCGTCGTGT AGATGGCATA GGACAGTGGC AAAATGTAAT GGAGGCGATG 
GGGGTTATAG CCATTATTGT AAACTGCTAC TTAATGGGCC AGTGTGGACA GCTTCAAAGA 
CTGCTACCCT GGCTGGGGCC CGAAACAACA ATCATATTTA TTGTGGTGCT TGAGCATTTT 
GCTTTGCTCC TGAAGTATAT AATACACGTT GTGATCCCAG ACATCCCTGG ATGGGTGGCA 
GAGGAGATGG CAAAACTGGA ATATCAAAGA AGAGAAGCTT TTAAGAAACA TGAGCGACAA 
GCTCAGCATC GCTACCAGCA GCAACAGCGC AGGAAGCGTG AGGAGGAGGA GAGACAGCGC 
CATGCTGATT ACCATGCGAG GAAAGAGCGA GAATCTCGTG GAGGGGACGA TGGAAAAAAC 
TCTGGACAAG ATTCCCAGAA TCATGAAAAG AACTCAAGCA AAACCAAGCA AGGTGGCCCC 
CCTGGAGGGA GCAGCCATGA TAAGCCGAAA AGACCCAGCT CCCTCTTGGC CAGCAATAAT 
GTGATGAAAC TTAAACAAAT TATTCCCCTG CAAAGCAAGT TCTTATCAGG GACCAATGCC 
AAATCTCCAC AGTCCCCTCC AGCGACAGAC ACCAAAATCC CCAGCTTTCT GAGCTTCAAA 
TTTCTAAAAT CCCCTGAAAC AAAAAGAGAA TCCTCCCAAG AGAGAGCCCA TTCTCCTACA 
AAGCCTTTCC ACCCAGGCAA GCTCTTTAAC TTTGGCGGAA AGGGTGAGGG ACAGGTGGGT 
GGTAATGGTA TTGGTCCACC CATATCAACA CAGCCAAGAG GAGCAACAGC TGGGACTGCC 
TCCTCTGATG GTACAGTGCC AGGTAAGTCC CAGCTAAATG GGATTTCAGA GGATAGACCC 
ACGGAGGAGG GTGAAGAACA AACCAAGGCA CAAAAACAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ano8 ( XM_004911016.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911073.1 Xenopus tropicalis anoctamin 8 (ano8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa04340
Clone ID Related Accession (Same CDS sequence)	XM_004911016.3 , XM_004911017.3 , XM_004911016.4 , XM_004911017.4
Accession Version	XM_004911016.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	anoctamin-8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911016.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGACA CAGAGCCGGA CAGAGGCAAG AAAGCCCAGC CGGAAGGGGA GCGAGCAGAA 
CCGGCTGGCA GCCAGCATGG CACGACTGGA GTACTGGATA AACTTTTTGG GAAGCGTCTT 
CTGCAAGCTG GTCGATATAT CACATCCCAC AAATCCTGGA TGAGGACTGT ACCCACAGAA 
AACTGTGACG TAGTTATGAC GTTCTCTGAT AACACGGATG ACCACACTTT GCTGTGGCTA 
CTGAATCATG TACGCCTGGG GTTACCTGAA CTTATTATGC AGATTCGACA CCACAGACAC 
ACTGGGATCT ACGCCTTCTT TGTAACTGCC ACATATGAAA ATTTGCTCCG CGGTGCGGAG 
GAGATTGGTC TTCGAAAACC AGTTAAAGCA GAGTTTGGAG GTGGAACACG AGTCTTCTCA 
TGCGAAGAGG ATTATATTTA TCAGAACATT GAAAATGAAC TCATTTTTTT CACATCTCAG 
GAACGTCAAA GCATCATCCG CTACTGGATG GAAAACCTCC GAGCCAAACA AGGCGAATCC 
CTGCACAATA TCCACTTCCT TGAGGGCCAG CCAATCATCC CAGAGTTGAC AGCCCGTGGG 
GTCATCCAGC AAGTGTTTCC CATCCATGAA CAAAGAATAT TAAGCCGACT TATGAGGTCC 
TGGGTCCAAG CAATCTGCGA AAAACAACCC CTTGATGAAA TCTGTGACTA TTTTGGGGTG 
AAAGTTGCCA TGTATTTTTC CTGGATGGGT TTCTACACCT CCTCCCTTGT CTATCCAGCT 
GTCTTTGGCA TGATGCTGTG GATGATCACT GAGTCTGACC AGACAAGCCG TGACATTTGC 
TGTGTTGTCT TTGCCCTTTT CAATGTTGTT TGGGCTACAT TATTCTTAGA GGGATGGAAA 
AGGAGAGGGG CAGAGTTGGC GTACAAGTGG GGGACACTGG ACACCCCTGC TGAATTTATT 
GAGGAGCCAA GGCCCCAATT CAGGGGTGTC AAGAGGATCA GTCCAGTCAC TAACTGCGAA 
GAGTTTTATT ACCCGCCGTG GAAACGCCTT TTGTTCCAGT CCTTTGTCAG TCTCCCTGTC 
TGTGTCTCCT GTCTGTGTTT TGTCTTTGTG GTCATGCTTG CCTGCTTTGA GCTTCAGGAG 
TTTATACTTA GTATCAAGGA GCTCCCACGG GTTGTACGTT TCTTTCCCAA GATTCTCCTG 
GCCGTTATAG TTAGCTCATG TGACGAAGTT TACAGGAAGA TTGCCTACTG GCTAAATGAT 
ATGGAAAATT ATCGTTTACA GAGTGCCTAC GAGAAACATC TCATCATTAA AATTGTTTTA 
TTCCAGTTTG TAAATTCCTA CCTGAGTCTT TTCTACATTG GTTTCTACCT CAAGGACATG 
GAGCGACTGA AAGAGCTGTT CCTCGTTTTG TCTCTCCCCC ATAGCTTCAC TCGTCAGGTT 
AAAGATGCCC TCCTCCCTTT CCTCTCACTC CAGTTTCACC TGTTCCTCAT CGTCCTCAGG 
GCACTTCTCA GATCAGCCTG GTGCCTCTTC AAATCTAAAA TGCTTGCCAC GCTCCTGGTC 
ATTCGCCAGT TCCTGCAGAA TGTGAAAGAA GTGTCCCAAC CTCATCTATA CCAGAAACTA 
CAACGGGGAG AGCTGACTCC ACGGTCAGTC TGGCTCTTTG TTCGCTATGC CCTGAAGAGG 
TTGTTTCAAA AATATTCTCT CCCTCAGCAC CCATTAAGGA CTCCTGGGAA CCCAATGGAG 
ACGGGTGCAG GTGGGCGCAA GAAGTGCCTC AATGGAGGGT GTGGTGTACC AGAAGAGGAG 
GAGGGAGTGG TCTCTGAAGA TGAGGAAGAG GAGGAGGAAG AAAGTATTAT GGATTGTGGA 
CTCAAATTAA AAAGGGTTAG TTTTTTAGAG AAAGGGGAGA GGAGAGAGGA TGTTGGTCTA 
GTTCCTCAGC AAGAAGATGA GGGTGCCTTT TTACAGGAGG GTAGTCCTAC TCTTATGGAA 
AGAGGTAGCG ACCCAGCCTC AGTTTTTCAG CTGGAAGATG AAGAGGATGA GGAACAAGAA 
GGAGCAAGTG GTGGAGTACT GCAGGAGGGT TTAGAGCAAG AGTCCGGGGT AAAGCTGAGG 
CAGCACAGAA GGAGGAGAAA GAGGGTAATT CTGGAGGAAG AACAAAGTAG AGAAGATGAG 
GAGGATGATA CAGAGATGAA GCAGAAAAGA GAATCATGGA TTGACCCTCC AGAGGACAAT 
TATCCAACTA AGCTTTCTCA AGCTGAGGTG GAGAGTTGTA TGAAGAAATA TGAGGACACT 
TTCAACGATT ACCAAGAGAT GTTTGTGCAG TTTGGGTATG TGGTCCTCTT TTCCTCGGCC 
TTCCCCTTGG CTGCTGTCTG TGCCCTGATC AATAACATAA TCGAGATCCG CAGTGATGCC 
TTTAAGCTGT GCACTGGGCT TCAGAGACCA TTTGGGCGTC GTGTAGATGG CATAGGACAG 
TGGCAAAATG TAATGGAGGC GATGGGGGTT ATAGCCATTA TTGTAAACTG CTACTTAATG 
GGCCAGTGTG GACAGCTTCA AAGACTGCTA CCCTGGCTGG GGCCCGAAAC AACAATCATA 
TTTATTGTGG TGCTTGAGCA TTTTGCTTTG CTCCTGAAGT ATATAATACA CGTTGTGATC 
CCAGACATCC CTGGATGGGT GGCAGAGGAG ATGGCAAAAC TGGAATATCA AAGAAGAGAA 
GCTTTTAAGA AACATGAGCG ACAAGCTCAG CATCGCTACC AGCAGCAACA GCGCAGGAAG 
CGTGAGGAGG AGGAGAGACA GCGCCATGCT GATTACCATG CGAGGAAAGA GCGAGAATCT 
CGTGGAGGGG ACGATGGAAA AAACTCTGGA CAAGATTCCC AGAATCATGA AAAGAACTCA 
AGCAAAACCA AGCAAGGTGG CCCCCCTGGA GGGAGCAGCC ATGATAAGCC GAAAAGACCC 
AGCTCCCTCT TGGCCAGCAA TAATGTGATG AAACTTAAAC AAATTATTCC CCTGCAAAGC 
AAGTTCTTAT CAGGGACCAA TGCCAAATCT CCACAGTCCC CTCCAGCGAC AGACACCAAA 
ATCCCCAGCT TTCTGAGCTT CAAATTTCTA AAATCCCCTG AAACAAAAAG AGAATCCTCC 
CAAGAGAGAG CCCATTCTCC TACAAAGCCT TTCCACCCAG GCAAGCTCTT TAACTTTGGC 
GGAAAGGGTG AGGGACAGGT GGGTGGTAAT GGTATTGGTC CACCCATATC AACACAGCCA 
AGAGGAGCAA CAGCTGGGAC TGCCTCCTCT GATGGTACAG TGCCAGGTAA GTCCCAGCTA 
AATGGGATTT CAGAGGATAG ACCCACGGAG GAGGGTGAAG AACAAACCAA GGCACAAAAA 
CAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911073.1
CDS	519..3884
Translation	MPDTEPDRGKKAQPEGERAEPAGSQHGTTGVLDKLFGKRLLQAGRYITSHKSWMRTVPTENCDVVMTFSDNTDDHTLLWLLNHVRLGLPELIMQIRHHRHTGIYAFFVTATYENLLRGAEEIGLRKPVKAEFGGGTRVFSCEEDYIYQNIENELIFFTSQERQSIIRYWMENLRAKQGESLHNIHFLEGQPIIPELTARGVIQQVFPIHEQRILSRLMRSWVQAICEKQPLDEICDYFGVKVAMYFSWMGFYTSSLVYPAVFGMMLWMITESDQTSRDICCVVFALFNVVWATLFLEGWKRRGAELAYKWGTLDTPAEFIEEPRPQFRGVKRISPVTNCEEFYYPPWKRLLFQSFVSLPVCVSCLCFVFVVMLACFELQEFILSIKELPRVVRFFPKILLAVIVSSCDEVYRKIAYWLNDMENYRLQSAYEKHLIIKIVLFQFVNSYLSLFYIGFYLKDMERLKELFLVLSLPHSFTRQVKDALLPFLSLQFHLFLIVLRALLRSAWCLFKSKMLATLLVIRQFLQNVKEVSQPHLYQKLQRGELTPRSVWLFVRYALKRLFQKYSLPQHPLRTPGNPMETGAGGRKKCLNGGCGVPEEEEGVVSEDEEEEEEESIMDCGLKLKRVSFLEKGERREDVGLVPQQEDEGAFLQEGSPTLMERGSDPASVFQLEDEEDEEQEGASGGVLQEGLEQESGVKLRQHRRRRKRVILEEEQSREDEEDDTEMKQKRESWIDPPEDNYPTKLSQAEVESCMKKYEDTFNDYQEMFVQFGYVVLFSSAFPLAAVCALINNIIEIRSDAFKLCTGLQRPFGRRVDGIGQWQNVMEAMGVIAIIVNCYLMGQCGQLQRLLPWLGPETTIIFIVVLEHFALLLKYIIHVVIPDIPGWVAEEMAKLEYQRREAFKKHERQAQHRYQQQQRRKREEEERQRHADYHARKERESRGGDDGKNSGQDSQNHEKNSSKTKQGGPPGGSSHDKPKRPSSLLASNNVMKLKQIIPLQSKFLSGTNAKSPQSPPATDTKIPSFLSFKFLKSPETKRESSQERAHSPTKPFHPGKLFNFGGKGEGQVGGNGIGPPISTQPRGATAGTASSDGTVPGKSQLNGISEDRPTEEGEEQTKAQKQ

Target ORF information:

RefSeq Version	XM_004911016.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis anoctamin 8 (ano8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911016.3

ORF Insert Sequence:

ATGCCGGACA CAGAGCCGGA CAGAGGCAAG AAAGCCCAGC CGGAAGGGGA GCGAGCAGAA 
CCGGCTGGCA GCCAGCATGG CACGACTGGA GTACTGGATA AACTTTTTGG GAAGCGTCTT 
CTGCAAGCTG GTCGATATAT CACATCCCAC AAATCCTGGA TGAGGACTGT ACCCACAGAA 
AACTGTGACG TAGTTATGAC GTTCTCTGAT AACACGGATG ACCACACTTT GCTGTGGCTA 
CTGAATCATG TACGCCTGGG GTTACCTGAA CTTATTATGC AGATTCGACA CCACAGACAC 
ACTGGGATCT ACGCCTTCTT TGTAACTGCC ACATATGAAA ATTTGCTCCG CGGTGCGGAG 
GAGATTGGTC TTCGAAAACC AGTTAAAGCA GAGTTTGGAG GTGGAACACG AGTCTTCTCA 
TGCGAAGAGG ATTATATTTA TCAGAACATT GAAAATGAAC TCATTTTTTT CACATCTCAG 
GAACGTCAAA GCATCATCCG CTACTGGATG GAAAACCTCC GAGCCAAACA AGGCGAATCC 
CTGCACAATA TCCACTTCCT TGAGGGCCAG CCAATCATCC CAGAGTTGAC AGCCCGTGGG 
GTCATCCAGC AAGTGTTTCC CATCCATGAA CAAAGAATAT TAAGCCGACT TATGAGGTCC 
TGGGTCCAAG CAATCTGCGA AAAACAACCC CTTGATGAAA TCTGTGACTA TTTTGGGGTG 
AAAGTTGCCA TGTATTTTTC CTGGATGGGT TTCTACACCT CCTCCCTTGT CTATCCAGCT 
GTCTTTGGCA TGATGCTGTG GATGATCACT GAGTCTGACC AGACAAGCCG TGACATTTGC 
TGTGTTGTCT TTGCCCTTTT CAATGTTGTT TGGGCTACAT TATTCTTAGA GGGATGGAAA 
AGGAGAGGGG CAGAGTTGGC GTACAAGTGG GGGACACTGG ACACCCCTGC TGAATTTATT 
GAGGAGCCAA GGCCCCAATT CAGGGGTGTC AAGAGGATCA GTCCAGTCAC TAACTGCGAA 
GAGTTTTATT ACCCGCCGTG GAAACGCCTT TTGTTCCAGT CCTTTGTCAG TCTCCCTGTC 
TGTGTCTCCT GTCTGTGTTT TGTCTTTGTG GTCATGCTTG CCTGCTTTGA GCTTCAGGAG 
TTTATACTTA GTATCAAGGA GCTCCCACGG GTTGTACGTT TCTTTCCCAA GATTCTCCTG 
GCCGTTATAG TTAGCTCATG TGACGAAGTT TACAGGAAGA TTGCCTACTG GCTAAATGAT 
ATGGAAAATT ATCGTTTACA GAGTGCCTAC GAGAAACATC TCATCATTAA AATTGTTTTA 
TTCCAGTTTG TAAATTCCTA CCTGAGTCTT TTCTACATTG GTTTCTACCT CAAGGACATG 
GAGCGACTGA AAGAGCTGTT CCTCGTTTTG TCTCTCCCCC ATAGCTTCAC TCGTCAGGTT 
AAAGATGCCC TCCTCCCTTT CCTCTCACTC CAGTTTCACC TGTTCCTCAT CGTCCTCAGG 
GCACTTCTCA GATCAGCCTG GTGCCTCTTC AAATCTAAAA TGCTTGCCAC GCTCCTGGTC 
ATTCGCCAGT TCCTGCAGAA TGTGAAAGAA GTGTCCCAAC CTCATCTATA CCAGAAACTA 
CAACGGGGAG AGCTGACTCC ACGGTCAGTC TGGCTCTTTG TTCGCTATGC CCTGAAGAGG 
TTGTTTCAAA AATATTCTCT CCCTCAGCAC CCATTAAGGA CTCCTGGGAA CCCAATGGAG 
ACGGGTGCAG GTGGGCGCAA GAAGTGCCTC AATGGAGGGT GTGGTGTACC AGAAGAGGAG 
GAGGGAGTGG TCTCTGAAGA TGAGGAAGAG GAGGAGGAAG AAAGTATTAT GGATTGTGGA 
CTCAAATTAA AAAGGGTTAG TTTTTTAGAG AAAGGGGAGA GGAGAGAGGA TGTTGGTCTA 
GTTCCTCAGC AAGAAGATGA GGGTGCCTTT TTACAGGAGG GTAGTCCTAC TCTTATGGAA 
AGAGGTAGCG ACCCAGCCTC AGTTTTTCAG CTGGAAGATG AAGAGGATGA GGAACAAGAA 
GGAGCAAGTG GTGGAGTACT GCAGGAGGGT TTAGAGCAAG AGTCCGGGGT AAAGCTGAGG 
CAGCACAGAA GGAGGAGAAA GAGGGTAATT CTGGAGGAAG AACAAAGTAG AGAAGATGAG 
GAGGATGATA CAGAGATGAA GCAGAAAAGA GAATCATGGA TTGACCCTCC AGAGGACAAT 
TATCCAACTA AGCTTTCTCA AGCTGAGGTG GAGAGTTGTA TGAAGAAATA TGAGGACACT 
TTCAACGATT ACCAAGAGAT GTTTGTGCAG TTTGGGTATG TGGTCCTCTT TTCCTCGGCC 
TTCCCCTTGG CTGCTGTCTG TGCCCTGATC AATAACATAA TCGAGATCCG CAGTGATGCC 
TTTAAGCTGT GCACTGGGCT TCAGAGACCA TTTGGGCGTC GTGTAGATGG CATAGGACAG 
TGGCAAAATG TAATGGAGGC GATGGGGGTT ATAGCCATTA TTGTAAACTG CTACTTAATG 
GGCCAGTGTG GACAGCTTCA AAGACTGCTA CCCTGGCTGG GGCCCGAAAC AACAATCATA 
TTTATTGTGG TGCTTGAGCA TTTTGCTTTG CTCCTGAAGT ATATAATACA CGTTGTGATC 
CCAGACATCC CTGGATGGGT GGCAGAGGAG ATGGCAAAAC TGGAATATCA AAGAAGAGAA 
GCTTTTAAGA AACATGAGCG ACAAGCTCAG CATCGCTACC AGCAGCAACA GCGCAGGAAG 
CGTGAGGAGG AGGAGAGACA GCGCCATGCT GATTACCATG CGAGGAAAGA GCGAGAATCT 
CGTGGAGGGG ACGATGGAAA AAACTCTGGA CAAGATTCCC AGAATCATGA AAAGAACTCA 
AGCAAAACCA AGCAAGGTGG CCCCCCTGGA GGGAGCAGCC ATGATAAGCC GAAAAGACCC 
AGCTCCCTCT TGGCCAGCAA TAATGTGATG AAACTTAAAC AAATTATTCC CCTGCAAAGC 
AAGTTCTTAT CAGGGACCAA TGCCAAATCT CCACAGTCCC CTCCAGCGAC AGACACCAAA 
ATCCCCAGCT TTCTGAGCTT CAAATTTCTA AAATCCCCTG AAACAAAAAG AGAATCCTCC 
CAAGAGAGAG CCCATTCTCC TACAAAGCCT TTCCACCCAG GCAAGCTCTT TAACTTTGGC 
GGAAAGGGTG AGGGACAGGT GGGTGGTAAT GGTATTGGTC CACCCATATC AACACAGCCA 
AGAGGAGCAA CAGCTGGGAC TGCCTCCTCT GATGGTACAG TGCCAGGTAA GTCCCAGCTA 
AATGGGATTT CAGAGGATAG ACCCACGGAG GAGGGTGAAG AACAAACCAA GGCACAAAAA 
CAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ano8 ( XM_004911017.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911074.1 Xenopus tropicalis anoctamin 8 (ano8), transcript variant X2, mRNA.

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CloneID	OXa04340
Clone ID Related Accession (Same CDS sequence)	XM_004911016.3 , XM_004911017.3 , XM_004911016.4 , XM_004911017.4
Accession Version	XM_004911017.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	anoctamin-8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911017.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGACA CAGAGCCGGA CAGAGGCAAG AAAGCCCAGC CGGAAGGGGA GCGAGCAGAA 
CCGGCTGGCA GCCAGCATGG CACGACTGGA GTACTGGATA AACTTTTTGG GAAGCGTCTT 
CTGCAAGCTG GTCGATATAT CACATCCCAC AAATCCTGGA TGAGGACTGT ACCCACAGAA 
AACTGTGACG TAGTTATGAC GTTCTCTGAT AACACGGATG ACCACACTTT GCTGTGGCTA 
CTGAATCATG TACGCCTGGG GTTACCTGAA CTTATTATGC AGATTCGACA CCACAGACAC 
ACTGGGATCT ACGCCTTCTT TGTAACTGCC ACATATGAAA ATTTGCTCCG CGGTGCGGAG 
GAGATTGGTC TTCGAAAACC AGTTAAAGCA GAGTTTGGAG GTGGAACACG AGTCTTCTCA 
TGCGAAGAGG ATTATATTTA TCAGAACATT GAAAATGAAC TCATTTTTTT CACATCTCAG 
GAACGTCAAA GCATCATCCG CTACTGGATG GAAAACCTCC GAGCCAAACA AGGCGAATCC 
CTGCACAATA TCCACTTCCT TGAGGGCCAG CCAATCATCC CAGAGTTGAC AGCCCGTGGG 
GTCATCCAGC AAGTGTTTCC CATCCATGAA CAAAGAATAT TAAGCCGACT TATGAGGTCC 
TGGGTCCAAG CAATCTGCGA AAAACAACCC CTTGATGAAA TCTGTGACTA TTTTGGGGTG 
AAAGTTGCCA TGTATTTTTC CTGGATGGGT TTCTACACCT CCTCCCTTGT CTATCCAGCT 
GTCTTTGGCA TGATGCTGTG GATGATCACT GAGTCTGACC AGACAAGCCG TGACATTTGC 
TGTGTTGTCT TTGCCCTTTT CAATGTTGTT TGGGCTACAT TATTCTTAGA GGGATGGAAA 
AGGAGAGGGG CAGAGTTGGC GTACAAGTGG GGGACACTGG ACACCCCTGC TGAATTTATT 
GAGGAGCCAA GGCCCCAATT CAGGGGTGTC AAGAGGATCA GTCCAGTCAC TAACTGCGAA 
GAGTTTTATT ACCCGCCGTG GAAACGCCTT TTGTTCCAGT CCTTTGTCAG TCTCCCTGTC 
TGTGTCTCCT GTCTGTGTTT TGTCTTTGTG GTCATGCTTG CCTGCTTTGA GCTTCAGGAG 
TTTATACTTA GTATCAAGGA GCTCCCACGG GTTGTACGTT TCTTTCCCAA GATTCTCCTG 
GCCGTTATAG TTAGCTCATG TGACGAAGTT TACAGGAAGA TTGCCTACTG GCTAAATGAT 
ATGGAAAATT ATCGTTTACA GAGTGCCTAC GAGAAACATC TCATCATTAA AATTGTTTTA 
TTCCAGTTTG TAAATTCCTA CCTGAGTCTT TTCTACATTG GTTTCTACCT CAAGGACATG 
GAGCGACTGA AAGAGCTGTT CCTCGTTTTG TCTCTCCCCC ATAGCTTCAC TCGTCAGGTT 
AAAGATGCCC TCCTCCCTTT CCTCTCACTC CAGTTTCACC TGTTCCTCAT CGTCCTCAGG 
GCACTTCTCA GATCAGCCTG GTGCCTCTTC AAATCTAAAA TGCTTGCCAC GCTCCTGGTC 
ATTCGCCAGT TCCTGCAGAA TGTGAAAGAA GTGTCCCAAC CTCATCTATA CCAGAAACTA 
CAACGGGGAG AGCTGACTCC ACGGTCAGTC TGGCTCTTTG TTCGCTATGC CCTGAAGAGG 
TTGTTTCAAA AATATTCTCT CCCTCAGCAC CCATTAAGGA CTCCTGGGAA CCCAATGGAG 
ACGGGTGCAG GTGGGCGCAA GAAGTGCCTC AATGGAGGGT GTGGTGTACC AGAAGAGGAG 
GAGGGAGTGG TCTCTGAAGA TGAGGAAGAG GAGGAGGAAG AAAGTATTAT GGATTGTGGA 
CTCAAATTAA AAAGGGTTAG TTTTTTAGAG AAAGGGGAGA GGAGAGAGGA TGTTGGTCTA 
GTTCCTCAGC AAGAAGATGA GGGTGCCTTT TTACAGGAGG GTAGTCCTAC TCTTATGGAA 
AGAGGTAGCG ACCCAGCCTC AGTTTTTCAG CTGGAAGATG AAGAGGATGA GGAACAAGAA 
GGAGCAAGTG GTGGAGTACT GCAGGAGGGT TTAGAGCAAG AGTCCGGGGT AAAGCTGAGG 
CAGCACAGAA GGAGGAGAAA GAGGGTAATT CTGGAGGAAG AACAAAGTAG AGAAGATGAG 
GAGGATGATA CAGAGATGAA GCAGAAAAGA GAATCATGGA TTGACCCTCC AGAGGACAAT 
TATCCAACTA AGCTTTCTCA AGCTGAGGTG GAGAGTTGTA TGAAGAAATA TGAGGACACT 
TTCAACGATT ACCAAGAGAT GTTTGTGCAG TTTGGGTATG TGGTCCTCTT TTCCTCGGCC 
TTCCCCTTGG CTGCTGTCTG TGCCCTGATC AATAACATAA TCGAGATCCG CAGTGATGCC 
TTTAAGCTGT GCACTGGGCT TCAGAGACCA TTTGGGCGTC GTGTAGATGG CATAGGACAG 
TGGCAAAATG TAATGGAGGC GATGGGGGTT ATAGCCATTA TTGTAAACTG CTACTTAATG 
GGCCAGTGTG GACAGCTTCA AAGACTGCTA CCCTGGCTGG GGCCCGAAAC AACAATCATA 
TTTATTGTGG TGCTTGAGCA TTTTGCTTTG CTCCTGAAGT ATATAATACA CGTTGTGATC 
CCAGACATCC CTGGATGGGT GGCAGAGGAG ATGGCAAAAC TGGAATATCA AAGAAGAGAA 
GCTTTTAAGA AACATGAGCG ACAAGCTCAG CATCGCTACC AGCAGCAACA GCGCAGGAAG 
CGTGAGGAGG AGGAGAGACA GCGCCATGCT GATTACCATG CGAGGAAAGA GCGAGAATCT 
CGTGGAGGGG ACGATGGAAA AAACTCTGGA CAAGATTCCC AGAATCATGA AAAGAACTCA 
AGCAAAACCA AGCAAGGTGG CCCCCCTGGA GGGAGCAGCC ATGATAAGCC GAAAAGACCC 
AGCTCCCTCT TGGCCAGCAA TAATGTGATG AAACTTAAAC AAATTATTCC CCTGCAAAGC 
AAGTTCTTAT CAGGGACCAA TGCCAAATCT CCACAGTCCC CTCCAGCGAC AGACACCAAA 
ATCCCCAGCT TTCTGAGCTT CAAATTTCTA AAATCCCCTG AAACAAAAAG AGAATCCTCC 
CAAGAGAGAG CCCATTCTCC TACAAAGCCT TTCCACCCAG GCAAGCTCTT TAACTTTGGC 
GGAAAGGGTG AGGGACAGGT GGGTGGTAAT GGTATTGGTC CACCCATATC AACACAGCCA 
AGAGGAGCAA CAGCTGGGAC TGCCTCCTCT GATGGTACAG TGCCAGGTAA GTCCCAGCTA 
AATGGGATTT CAGAGGATAG ACCCACGGAG GAGGGTGAAG AACAAACCAA GGCACAAAAA 
CAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911074.1
CDS	273..3638
Translation	MPDTEPDRGKKAQPEGERAEPAGSQHGTTGVLDKLFGKRLLQAGRYITSHKSWMRTVPTENCDVVMTFSDNTDDHTLLWLLNHVRLGLPELIMQIRHHRHTGIYAFFVTATYENLLRGAEEIGLRKPVKAEFGGGTRVFSCEEDYIYQNIENELIFFTSQERQSIIRYWMENLRAKQGESLHNIHFLEGQPIIPELTARGVIQQVFPIHEQRILSRLMRSWVQAICEKQPLDEICDYFGVKVAMYFSWMGFYTSSLVYPAVFGMMLWMITESDQTSRDICCVVFALFNVVWATLFLEGWKRRGAELAYKWGTLDTPAEFIEEPRPQFRGVKRISPVTNCEEFYYPPWKRLLFQSFVSLPVCVSCLCFVFVVMLACFELQEFILSIKELPRVVRFFPKILLAVIVSSCDEVYRKIAYWLNDMENYRLQSAYEKHLIIKIVLFQFVNSYLSLFYIGFYLKDMERLKELFLVLSLPHSFTRQVKDALLPFLSLQFHLFLIVLRALLRSAWCLFKSKMLATLLVIRQFLQNVKEVSQPHLYQKLQRGELTPRSVWLFVRYALKRLFQKYSLPQHPLRTPGNPMETGAGGRKKCLNGGCGVPEEEEGVVSEDEEEEEEESIMDCGLKLKRVSFLEKGERREDVGLVPQQEDEGAFLQEGSPTLMERGSDPASVFQLEDEEDEEQEGASGGVLQEGLEQESGVKLRQHRRRRKRVILEEEQSREDEEDDTEMKQKRESWIDPPEDNYPTKLSQAEVESCMKKYEDTFNDYQEMFVQFGYVVLFSSAFPLAAVCALINNIIEIRSDAFKLCTGLQRPFGRRVDGIGQWQNVMEAMGVIAIIVNCYLMGQCGQLQRLLPWLGPETTIIFIVVLEHFALLLKYIIHVVIPDIPGWVAEEMAKLEYQRREAFKKHERQAQHRYQQQQRRKREEEERQRHADYHARKERESRGGDDGKNSGQDSQNHEKNSSKTKQGGPPGGSSHDKPKRPSSLLASNNVMKLKQIIPLQSKFLSGTNAKSPQSPPATDTKIPSFLSFKFLKSPETKRESSQERAHSPTKPFHPGKLFNFGGKGEGQVGGNGIGPPISTQPRGATAGTASSDGTVPGKSQLNGISEDRPTEEGEEQTKAQKQ

Target ORF information:

RefSeq Version	XM_004911017.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis anoctamin 8 (ano8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911017.3

ORF Insert Sequence:

ATGCCGGACA CAGAGCCGGA CAGAGGCAAG AAAGCCCAGC CGGAAGGGGA GCGAGCAGAA 
CCGGCTGGCA GCCAGCATGG CACGACTGGA GTACTGGATA AACTTTTTGG GAAGCGTCTT 
CTGCAAGCTG GTCGATATAT CACATCCCAC AAATCCTGGA TGAGGACTGT ACCCACAGAA 
AACTGTGACG TAGTTATGAC GTTCTCTGAT AACACGGATG ACCACACTTT GCTGTGGCTA 
CTGAATCATG TACGCCTGGG GTTACCTGAA CTTATTATGC AGATTCGACA CCACAGACAC 
ACTGGGATCT ACGCCTTCTT TGTAACTGCC ACATATGAAA ATTTGCTCCG CGGTGCGGAG 
GAGATTGGTC TTCGAAAACC AGTTAAAGCA GAGTTTGGAG GTGGAACACG AGTCTTCTCA 
TGCGAAGAGG ATTATATTTA TCAGAACATT GAAAATGAAC TCATTTTTTT CACATCTCAG 
GAACGTCAAA GCATCATCCG CTACTGGATG GAAAACCTCC GAGCCAAACA AGGCGAATCC 
CTGCACAATA TCCACTTCCT TGAGGGCCAG CCAATCATCC CAGAGTTGAC AGCCCGTGGG 
GTCATCCAGC AAGTGTTTCC CATCCATGAA CAAAGAATAT TAAGCCGACT TATGAGGTCC 
TGGGTCCAAG CAATCTGCGA AAAACAACCC CTTGATGAAA TCTGTGACTA TTTTGGGGTG 
AAAGTTGCCA TGTATTTTTC CTGGATGGGT TTCTACACCT CCTCCCTTGT CTATCCAGCT 
GTCTTTGGCA TGATGCTGTG GATGATCACT GAGTCTGACC AGACAAGCCG TGACATTTGC 
TGTGTTGTCT TTGCCCTTTT CAATGTTGTT TGGGCTACAT TATTCTTAGA GGGATGGAAA 
AGGAGAGGGG CAGAGTTGGC GTACAAGTGG GGGACACTGG ACACCCCTGC TGAATTTATT 
GAGGAGCCAA GGCCCCAATT CAGGGGTGTC AAGAGGATCA GTCCAGTCAC TAACTGCGAA 
GAGTTTTATT ACCCGCCGTG GAAACGCCTT TTGTTCCAGT CCTTTGTCAG TCTCCCTGTC 
TGTGTCTCCT GTCTGTGTTT TGTCTTTGTG GTCATGCTTG CCTGCTTTGA GCTTCAGGAG 
TTTATACTTA GTATCAAGGA GCTCCCACGG GTTGTACGTT TCTTTCCCAA GATTCTCCTG 
GCCGTTATAG TTAGCTCATG TGACGAAGTT TACAGGAAGA TTGCCTACTG GCTAAATGAT 
ATGGAAAATT ATCGTTTACA GAGTGCCTAC GAGAAACATC TCATCATTAA AATTGTTTTA 
TTCCAGTTTG TAAATTCCTA CCTGAGTCTT TTCTACATTG GTTTCTACCT CAAGGACATG 
GAGCGACTGA AAGAGCTGTT CCTCGTTTTG TCTCTCCCCC ATAGCTTCAC TCGTCAGGTT 
AAAGATGCCC TCCTCCCTTT CCTCTCACTC CAGTTTCACC TGTTCCTCAT CGTCCTCAGG 
GCACTTCTCA GATCAGCCTG GTGCCTCTTC AAATCTAAAA TGCTTGCCAC GCTCCTGGTC 
ATTCGCCAGT TCCTGCAGAA TGTGAAAGAA GTGTCCCAAC CTCATCTATA CCAGAAACTA 
CAACGGGGAG AGCTGACTCC ACGGTCAGTC TGGCTCTTTG TTCGCTATGC CCTGAAGAGG 
TTGTTTCAAA AATATTCTCT CCCTCAGCAC CCATTAAGGA CTCCTGGGAA CCCAATGGAG 
ACGGGTGCAG GTGGGCGCAA GAAGTGCCTC AATGGAGGGT GTGGTGTACC AGAAGAGGAG 
GAGGGAGTGG TCTCTGAAGA TGAGGAAGAG GAGGAGGAAG AAAGTATTAT GGATTGTGGA 
CTCAAATTAA AAAGGGTTAG TTTTTTAGAG AAAGGGGAGA GGAGAGAGGA TGTTGGTCTA 
GTTCCTCAGC AAGAAGATGA GGGTGCCTTT TTACAGGAGG GTAGTCCTAC TCTTATGGAA 
AGAGGTAGCG ACCCAGCCTC AGTTTTTCAG CTGGAAGATG AAGAGGATGA GGAACAAGAA 
GGAGCAAGTG GTGGAGTACT GCAGGAGGGT TTAGAGCAAG AGTCCGGGGT AAAGCTGAGG 
CAGCACAGAA GGAGGAGAAA GAGGGTAATT CTGGAGGAAG AACAAAGTAG AGAAGATGAG 
GAGGATGATA CAGAGATGAA GCAGAAAAGA GAATCATGGA TTGACCCTCC AGAGGACAAT 
TATCCAACTA AGCTTTCTCA AGCTGAGGTG GAGAGTTGTA TGAAGAAATA TGAGGACACT 
TTCAACGATT ACCAAGAGAT GTTTGTGCAG TTTGGGTATG TGGTCCTCTT TTCCTCGGCC 
TTCCCCTTGG CTGCTGTCTG TGCCCTGATC AATAACATAA TCGAGATCCG CAGTGATGCC 
TTTAAGCTGT GCACTGGGCT TCAGAGACCA TTTGGGCGTC GTGTAGATGG CATAGGACAG 
TGGCAAAATG TAATGGAGGC GATGGGGGTT ATAGCCATTA TTGTAAACTG CTACTTAATG 
GGCCAGTGTG GACAGCTTCA AAGACTGCTA CCCTGGCTGG GGCCCGAAAC AACAATCATA 
TTTATTGTGG TGCTTGAGCA TTTTGCTTTG CTCCTGAAGT ATATAATACA CGTTGTGATC 
CCAGACATCC CTGGATGGGT GGCAGAGGAG ATGGCAAAAC TGGAATATCA AAGAAGAGAA 
GCTTTTAAGA AACATGAGCG ACAAGCTCAG CATCGCTACC AGCAGCAACA GCGCAGGAAG 
CGTGAGGAGG AGGAGAGACA GCGCCATGCT GATTACCATG CGAGGAAAGA GCGAGAATCT 
CGTGGAGGGG ACGATGGAAA AAACTCTGGA CAAGATTCCC AGAATCATGA AAAGAACTCA 
AGCAAAACCA AGCAAGGTGG CCCCCCTGGA GGGAGCAGCC ATGATAAGCC GAAAAGACCC 
AGCTCCCTCT TGGCCAGCAA TAATGTGATG AAACTTAAAC AAATTATTCC CCTGCAAAGC 
AAGTTCTTAT CAGGGACCAA TGCCAAATCT CCACAGTCCC CTCCAGCGAC AGACACCAAA 
ATCCCCAGCT TTCTGAGCTT CAAATTTCTA AAATCCCCTG AAACAAAAAG AGAATCCTCC 
CAAGAGAGAG CCCATTCTCC TACAAAGCCT TTCCACCCAG GCAAGCTCTT TAACTTTGGC 
GGAAAGGGTG AGGGACAGGT GGGTGGTAAT GGTATTGGTC CACCCATATC AACACAGCCA 
AGAGGAGCAA CAGCTGGGAC TGCCTCCTCT GATGGTACAG TGCCAGGTAA GTCCCAGCTA 
AATGGGATTT CAGAGGATAG ACCCACGGAG GAGGGTGAAG AACAAACCAA GGCACAAAAA 
CAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ano8 ( XM_002936335.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002936381.1 Xenopus tropicalis anoctamin 8 (ano8), transcript variant X3, mRNA.

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CloneID	OXa04341
Clone ID Related Accession (Same CDS sequence)	XM_002936335.5 , XM_002936335.4
Accession Version	XM_002936335.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	anoctamin-8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002936335.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGACA CAGAGCCGGA CAGAGGCAAG AAAGCCCAGC CGGAAGGGGA GCGAGCAGAA 
CCGGCTGGCA GCCAGCATGG CACGACTGGA GTACTGGATA AACTTTTTGG GAAGCGTCTT 
CTGCAAGCTG GTCGATATAT CACATCCCAC AAATCCTGGA TGAGGACTGT ACCCACAGAA 
AACTGTGACG TAGTTATGAC GTTCTCTGAT AACACGGATG ACCACACTTT GCTGTGGCTA 
CTGAATCATG TACGCCTGGG GTTACCTGAA CTTATTATGC AGATTCGACA CCACAGACAC 
ACTGGGATCT ACGCCTTCTT TGTAACTGCC ACATATGAAA ATTTGCTCCG CGGTGCGGAG 
GAGATTGGTC TTCGAAAACC AGTTAAAGCA GAGTTTGGAG GTGGAACACG AGTCTTCTCA 
TGCGAAGAGG ATTATATTTA TCAGAACATT GAAAATGAAC TCATTTTTTT CACATCTCAG 
GAACGTCAAA GCATCATCCG CTACTGGATG GAAAACCTCC GAGCCAAACA AGGCGAATCC 
CTGCACAATA TCCACTTCCT TGAGGGCCAG CCAATCATCC CAGAGTTGAC AGCCCGTGGG 
GTCATCCAGC AAGTGTTTCC CATCCATGAA CAAAGAATAT TAAGCCGACT TATGAGGTCC 
TGGGTCCAAG CAATCTGCGA AAAACAACCC CTTGATGAAA TCTGTGACTA TTTTGGGGTG 
AAAGTTGCCA TGTATTTTTC CTGGATGGGT TTCTACACCT CCTCCCTTGT CTATCCAGCT 
GTCTTTGGCA TGATGCTGTG GATGATCACT GAGTCTGACC AGACAAGCCG TGACATTTGC 
TGTGTTGTCT TTGCCCTTTT CAATGTTGTT TGGGCTACAT TATTCTTAGA GGGATGGAAA 
AGGAGAGGGG CAGAGTTGGC GTACAAGTGG GGGACACTGG ACACCCCTGC TGAATTTATT 
GAGGAGCCAA GGCCCCAATT CAGGGGTGTC AAGAGGATCA GTCCAGTCAC TAACTGCGAA 
GAGTTTTATT ACCCGCCGTG GAAACGCCTT TTGTTCCAGT CCTTTGTCAG TCTCCCTGTC 
TGTGTCTCCT GTCTGTGTTT TGTCTTTGTG GTCATGCTTG CCTGCTTTGA GCTTCAGGAG 
TTTATACTTA GTATCAAGGA GCTCCCACGG GTTGTACGTT TCTTTCCCAA GATTCTCCTG 
GCCGTTATAG TTAGCTCATG TGACGAAGTT TACAGGAAGA TTGCCTACTG GCTAAATGAT 
ATGGAAAATT ATCGTTTACA GAGTGCCTAC GAGAAACATC TCATCATTAA AATTGTTTTA 
TTCCAGTTTG TAAATTCCTA CCTGAGTCTT TTCTACATTG GTTTCTACCT CAAGGACATG 
GAGCGACTGA AAGAGATGCT TGCCACGCTC CTGGTCATTC GCCAGTTCCT GCAGAATGTG 
AAAGAAGTGT CCCAACCTCA TCTATACCAG AAACTACAAC GGGGAGAGCT GACTCCACGG 
TCAGTCTGGC TCTTTGTTCG CTATGCCCTG AAGAGGTTGT TTCAAAAATA TTCTCTCCCT 
CAGCACCCAT TAAGGACTCC TGGGAACCCA ATGGAGACGG GTGCAGGTGG GCGCAAGAAG 
TGCCTCAATG GAGGGTGTGG TGTACCAGAA GAGGAGGAGG GAGTGGTCTC TGAAGATGAG 
GAAGAGGAGG AGGAAGAAAG TATTATGGAT TGTGGACTCA AATTAAAAAG GGTTAGTTTT 
TTAGAGAAAG GGGAGAGGAG AGAGGATGTT GGTCTAGTTC CTCAGCAAGA AGATGAGGGT 
GCCTTTTTAC AGGAGGGTAG TCCTACTCTT ATGGAAAGAG GTAGCGACCC AGCCTCAGTT 
TTTCAGCTGG AAGATGAAGA GGATGAGGAA CAAGAAGGAG CAAGTGGTGG AGTACTGCAG 
GAGGGTTTAG AGCAAGAGTC CGGGGTAAAG CTGAGGCAGC ACAGAAGGAG GAGAAAGAGG 
GTAATTCTGG AGGAAGAACA AAGTAGAGAA GATGAGGAGG ATGATACAGA GATGAAGCAG 
AAAAGAGAAT CATGGATTGA CCCTCCAGAG GACAATTATC CAACTAAGCT TTCTCAAGCT 
GAGGTGGAGA GTTGTATGAA GAAATATGAG GACACTTTCA ACGATTACCA AGAGATGTTT 
GTGCAGTTTG GGTATGTGGT CCTCTTTTCC TCGGCCTTCC CCTTGGCTGC TGTCTGTGCC 
CTGATCAATA ACATAATCGA GATCCGCAGT GATGCCTTTA AGCTGTGCAC TGGGCTTCAG 
AGACCATTTG GGCGTCGTGT AGATGGCATA GGACAGTGGC AAAATGTAAT GGAGGCGATG 
GGGGTTATAG CCATTATTGT AAACTGCTAC TTAATGGGCC AGTGTGGACA GCTTCAAAGA 
CTGCTACCCT GGCTGGGGCC CGAAACAACA ATCATATTTA TTGTGGTGCT TGAGCATTTT 
GCTTTGCTCC TGAAGTATAT AATACACGTT GTGATCCCAG ACATCCCTGG ATGGGTGGCA 
GAGGAGATGG CAAAACTGGA ATATCAAAGA AGAGAAGCTT TTAAGAAACA TGAGCGACAA 
GCTCAGCATC GCTACCAGCA GCAACAGCGC AGGAAGCGTG AGGAGGAGGA GAGACAGCGC 
CATGCTGATT ACCATGCGAG GAAAGAGCGA GAATCTCGTG GAGGGGACGA TGGAAAAAAC 
TCTGGACAAG ATTCCCAGAA TCATGAAAAG AACTCAAGCA AAACCAAGCA AGGTGGCCCC 
CCTGGAGGGA GCAGCCATGA TAAGCCGAAA AGACCCAGCT CCCTCTTGGC CAGCAATAAT 
GTGATGAAAC TTAAACAAAT TATTCCCCTG CAAAGCAAGT TCTTATCAGG GACCAATGCC 
AAATCTCCAC AGTCCCCTCC AGCGACAGAC ACCAAAATCC CCAGCTTTCT GAGCTTCAAA 
TTTCTAAAAT CCCCTGAAAC AAAAAGAGAA TCCTCCCAAG AGAGAGCCCA TTCTCCTACA 
AAGCCTTTCC ACCCAGGCAA GCTCTTTAAC TTTGGCGGAA AGGGTGAGGG ACAGGTGGGT 
GGTAATGGTA TTGGTCCACC CATATCAACA CAGCCAAGAG GAGCAACAGC TGGGACTGCC 
TCCTCTGATG GTACAGTGCC AGGTAAGTCC CAGCTAAATG GGATTTCAGA GGATAGACCC 
ACGGAGGAGG GTGAAGAACA AACCAAGGCA CAAAAACAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002936381.1
CDS	519..3740
Translation	MPDTEPDRGKKAQPEGERAEPAGSQHGTTGVLDKLFGKRLLQAGRYITSHKSWMRTVPTENCDVVMTFSDNTDDHTLLWLLNHVRLGLPELIMQIRHHRHTGIYAFFVTATYENLLRGAEEIGLRKPVKAEFGGGTRVFSCEEDYIYQNIENELIFFTSQERQSIIRYWMENLRAKQGESLHNIHFLEGQPIIPELTARGVIQQVFPIHEQRILSRLMRSWVQAICEKQPLDEICDYFGVKVAMYFSWMGFYTSSLVYPAVFGMMLWMITESDQTSRDICCVVFALFNVVWATLFLEGWKRRGAELAYKWGTLDTPAEFIEEPRPQFRGVKRISPVTNCEEFYYPPWKRLLFQSFVSLPVCVSCLCFVFVVMLACFELQEFILSIKELPRVVRFFPKILLAVIVSSCDEVYRKIAYWLNDMENYRLQSAYEKHLIIKIVLFQFVNSYLSLFYIGFYLKDMERLKEMLATLLVIRQFLQNVKEVSQPHLYQKLQRGELTPRSVWLFVRYALKRLFQKYSLPQHPLRTPGNPMETGAGGRKKCLNGGCGVPEEEEGVVSEDEEEEEEESIMDCGLKLKRVSFLEKGERREDVGLVPQQEDEGAFLQEGSPTLMERGSDPASVFQLEDEEDEEQEGASGGVLQEGLEQESGVKLRQHRRRRKRVILEEEQSREDEEDDTEMKQKRESWIDPPEDNYPTKLSQAEVESCMKKYEDTFNDYQEMFVQFGYVVLFSSAFPLAAVCALINNIIEIRSDAFKLCTGLQRPFGRRVDGIGQWQNVMEAMGVIAIIVNCYLMGQCGQLQRLLPWLGPETTIIFIVVLEHFALLLKYIIHVVIPDIPGWVAEEMAKLEYQRREAFKKHERQAQHRYQQQQRRKREEEERQRHADYHARKERESRGGDDGKNSGQDSQNHEKNSSKTKQGGPPGGSSHDKPKRPSSLLASNNVMKLKQIIPLQSKFLSGTNAKSPQSPPATDTKIPSFLSFKFLKSPETKRESSQERAHSPTKPFHPGKLFNFGGKGEGQVGGNGIGPPISTQPRGATAGTASSDGTVPGKSQLNGISEDRPTEEGEEQTKAQKQ

Target ORF information:

RefSeq Version	XM_002936335.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis anoctamin 8 (ano8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002936335.4

ORF Insert Sequence:

ATGCCGGACA CAGAGCCGGA CAGAGGCAAG AAAGCCCAGC CGGAAGGGGA GCGAGCAGAA 
CCGGCTGGCA GCCAGCATGG CACGACTGGA GTACTGGATA AACTTTTTGG GAAGCGTCTT 
CTGCAAGCTG GTCGATATAT CACATCCCAC AAATCCTGGA TGAGGACTGT ACCCACAGAA 
AACTGTGACG TAGTTATGAC GTTCTCTGAT AACACGGATG ACCACACTTT GCTGTGGCTA 
CTGAATCATG TACGCCTGGG GTTACCTGAA CTTATTATGC AGATTCGACA CCACAGACAC 
ACTGGGATCT ACGCCTTCTT TGTAACTGCC ACATATGAAA ATTTGCTCCG CGGTGCGGAG 
GAGATTGGTC TTCGAAAACC AGTTAAAGCA GAGTTTGGAG GTGGAACACG AGTCTTCTCA 
TGCGAAGAGG ATTATATTTA TCAGAACATT GAAAATGAAC TCATTTTTTT CACATCTCAG 
GAACGTCAAA GCATCATCCG CTACTGGATG GAAAACCTCC GAGCCAAACA AGGCGAATCC 
CTGCACAATA TCCACTTCCT TGAGGGCCAG CCAATCATCC CAGAGTTGAC AGCCCGTGGG 
GTCATCCAGC AAGTGTTTCC CATCCATGAA CAAAGAATAT TAAGCCGACT TATGAGGTCC 
TGGGTCCAAG CAATCTGCGA AAAACAACCC CTTGATGAAA TCTGTGACTA TTTTGGGGTG 
AAAGTTGCCA TGTATTTTTC CTGGATGGGT TTCTACACCT CCTCCCTTGT CTATCCAGCT 
GTCTTTGGCA TGATGCTGTG GATGATCACT GAGTCTGACC AGACAAGCCG TGACATTTGC 
TGTGTTGTCT TTGCCCTTTT CAATGTTGTT TGGGCTACAT TATTCTTAGA GGGATGGAAA 
AGGAGAGGGG CAGAGTTGGC GTACAAGTGG GGGACACTGG ACACCCCTGC TGAATTTATT 
GAGGAGCCAA GGCCCCAATT CAGGGGTGTC AAGAGGATCA GTCCAGTCAC TAACTGCGAA 
GAGTTTTATT ACCCGCCGTG GAAACGCCTT TTGTTCCAGT CCTTTGTCAG TCTCCCTGTC 
TGTGTCTCCT GTCTGTGTTT TGTCTTTGTG GTCATGCTTG CCTGCTTTGA GCTTCAGGAG 
TTTATACTTA GTATCAAGGA GCTCCCACGG GTTGTACGTT TCTTTCCCAA GATTCTCCTG 
GCCGTTATAG TTAGCTCATG TGACGAAGTT TACAGGAAGA TTGCCTACTG GCTAAATGAT 
ATGGAAAATT ATCGTTTACA GAGTGCCTAC GAGAAACATC TCATCATTAA AATTGTTTTA 
TTCCAGTTTG TAAATTCCTA CCTGAGTCTT TTCTACATTG GTTTCTACCT CAAGGACATG 
GAGCGACTGA AAGAGATGCT TGCCACGCTC CTGGTCATTC GCCAGTTCCT GCAGAATGTG 
AAAGAAGTGT CCCAACCTCA TCTATACCAG AAACTACAAC GGGGAGAGCT GACTCCACGG 
TCAGTCTGGC TCTTTGTTCG CTATGCCCTG AAGAGGTTGT TTCAAAAATA TTCTCTCCCT 
CAGCACCCAT TAAGGACTCC TGGGAACCCA ATGGAGACGG GTGCAGGTGG GCGCAAGAAG 
TGCCTCAATG GAGGGTGTGG TGTACCAGAA GAGGAGGAGG GAGTGGTCTC TGAAGATGAG 
GAAGAGGAGG AGGAAGAAAG TATTATGGAT TGTGGACTCA AATTAAAAAG GGTTAGTTTT 
TTAGAGAAAG GGGAGAGGAG AGAGGATGTT GGTCTAGTTC CTCAGCAAGA AGATGAGGGT 
GCCTTTTTAC AGGAGGGTAG TCCTACTCTT ATGGAAAGAG GTAGCGACCC AGCCTCAGTT 
TTTCAGCTGG AAGATGAAGA GGATGAGGAA CAAGAAGGAG CAAGTGGTGG AGTACTGCAG 
GAGGGTTTAG AGCAAGAGTC CGGGGTAAAG CTGAGGCAGC ACAGAAGGAG GAGAAAGAGG 
GTAATTCTGG AGGAAGAACA AAGTAGAGAA GATGAGGAGG ATGATACAGA GATGAAGCAG 
AAAAGAGAAT CATGGATTGA CCCTCCAGAG GACAATTATC CAACTAAGCT TTCTCAAGCT 
GAGGTGGAGA GTTGTATGAA GAAATATGAG GACACTTTCA ACGATTACCA AGAGATGTTT 
GTGCAGTTTG GGTATGTGGT CCTCTTTTCC TCGGCCTTCC CCTTGGCTGC TGTCTGTGCC 
CTGATCAATA ACATAATCGA GATCCGCAGT GATGCCTTTA AGCTGTGCAC TGGGCTTCAG 
AGACCATTTG GGCGTCGTGT AGATGGCATA GGACAGTGGC AAAATGTAAT GGAGGCGATG 
GGGGTTATAG CCATTATTGT AAACTGCTAC TTAATGGGCC AGTGTGGACA GCTTCAAAGA 
CTGCTACCCT GGCTGGGGCC CGAAACAACA ATCATATTTA TTGTGGTGCT TGAGCATTTT 
GCTTTGCTCC TGAAGTATAT AATACACGTT GTGATCCCAG ACATCCCTGG ATGGGTGGCA 
GAGGAGATGG CAAAACTGGA ATATCAAAGA AGAGAAGCTT TTAAGAAACA TGAGCGACAA 
GCTCAGCATC GCTACCAGCA GCAACAGCGC AGGAAGCGTG AGGAGGAGGA GAGACAGCGC 
CATGCTGATT ACCATGCGAG GAAAGAGCGA GAATCTCGTG GAGGGGACGA TGGAAAAAAC 
TCTGGACAAG ATTCCCAGAA TCATGAAAAG AACTCAAGCA AAACCAAGCA AGGTGGCCCC 
CCTGGAGGGA GCAGCCATGA TAAGCCGAAA AGACCCAGCT CCCTCTTGGC CAGCAATAAT 
GTGATGAAAC TTAAACAAAT TATTCCCCTG CAAAGCAAGT TCTTATCAGG GACCAATGCC 
AAATCTCCAC AGTCCCCTCC AGCGACAGAC ACCAAAATCC CCAGCTTTCT GAGCTTCAAA 
TTTCTAAAAT CCCCTGAAAC AAAAAGAGAA TCCTCCCAAG AGAGAGCCCA TTCTCCTACA 
AAGCCTTTCC ACCCAGGCAA GCTCTTTAAC TTTGGCGGAA AGGGTGAGGG ACAGGTGGGT 
GGTAATGGTA TTGGTCCACC CATATCAACA CAGCCAAGAG GAGCAACAGC TGGGACTGCC 
TCCTCTGATG GTACAGTGCC AGGTAAGTCC CAGCTAAATG GGATTTCAGA GGATAGACCC 
ACGGAGGAGG GTGAAGAACA AACCAAGGCA CAAAAACAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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ano8 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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